26,832 results match your criteria content strain


Enhanced matrix production by cocultivated human stem cells and chondrocytes under concurrent mechanical strain.

In Vitro Cell Dev Biol Anim 2021 Jun 15. Epub 2021 Jun 15.

Voiland School of Chemical Engineering and Bioengineering, Washington State University, 1505 NE Stadium Way, Pullman, WA, 99164-6515, USA.

Conventional treatments of osteoarthritis have failed to re-build functional articular cartilage. Tissue engineering clinical treatments for osteoarthritis, including autologous chondrocyte implantation, provides an alternative approach by injecting a cell suspension to fill lesions within the cartilage in osteoarthritic knees. The success of chondrocyte implantation relies on the availability of chondrogenic cell lines, and their resilience to high mechanical loading. Read More

View Article and Full-Text PDF

sp. nov. a novel actinobacterium isolated from the coral .

Int J Syst Evol Microbiol 2021 Jun;71(6)

Key Laboratory of Tropical Biological Resources of the Ministry of Education of China, Hainan University, Haikou 570228, PR China.

Strain HNM0947, representing a novel actinobacterium, was isolated from the coral collected from the coast of Wenchang, Hainan, China. The strain was found to have morphological and chemotaxonomic characteristics consistent with the genus . The organism formed abundant fragmented substrate mycelia and aerial mycelia which differentiated into non-motile, rod-shaped spores. Read More

View Article and Full-Text PDF

Description of sp. nov., based on phylogenomic analysis.

Int J Syst Evol Microbiol 2021 Jun;71(6)

Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar - 751023, India.

A novel strain of a member of the genus strain PS-1, was isolated from the skin of fresh water pufferfish () collected from Mahanadi River, India. Cells were Gram-stain-negative, aerobic, coccoid and non-motile. The predominant polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phospholipid (PL) and the cell wall sugars were glucose, galactose and ribose. Read More

View Article and Full-Text PDF

Interaction Between the Intestinal Microbial Community and Transcriptome Profile in Common Carp ( L.).

Front Microbiol 2021 28;12:659602. Epub 2021 May 28.

Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.

In a previous study, we found that the growth performance of the new strain of Huanghe carp is related to gene expression and bacterial community in the gut. In order to better understand the relationship between the gene expression level and bacterial abundance in the gut, we studied the growth performance, gut bacterial structure, and transcriptome profile in the 4th generation of the new carp strain (selection group) at harvesting time, and compared them with the control line (traditional Huanghe carp). Body weight, depth, width, and length increased 14. Read More

View Article and Full-Text PDF

Fermentation of Saccharomyces cerevisiae in a 7.5 L ultrasound-enhanced fermenter: Effect of sonication conditions on ethanol production, intracellular Ca concentration and key regulating enzyme activity in glycolysis.

Ultrason Sonochem 2021 Jun 8;76:105624. Epub 2021 Jun 8.

School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China; Institute of Food Physical Processing, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.

In this study, the effect of sonication on the fermentation process of a single-celled fungus was examined. During the experiment, Saccharomyces cerevisiae (S. cerevisiae) was used as the starting strain for ethanol fermentation (batch fermentation) in a 7. Read More

View Article and Full-Text PDF

Selenium-oxidizing Agrobacterium sp. T3F4 steadily colonizes in soil promoting selenium uptake by pak choi (Brassica campestris).

Sci Total Environ 2021 Jun 4;791:148294. Epub 2021 Jun 4.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address:

Selenium (Se) deficiency in soil is linked to its low content in edible crops, resulting in adverse impacts on the health of 15% of the global population. The crop mainly absorbs oxidized selenate and selenite from soil, then converts them into organic Se. However, the role of Se-oxidizing bacteria in soil Se oxidation, Se bioavailability and Se absorption into plants remains unclear. Read More

View Article and Full-Text PDF

sp. nov., isolated from swine faeces.

Int J Syst Evol Microbiol 2021 Jun;71(6)

Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.

An obligately anaerobic, Gram-positive, non-motile, coccus-shaped bacterial strain designated AGMB00490 was isolated from swine faeces. 16S rRNA gene sequence-based phylogenetic analysis indicated that the isolate belongs to the genus and that the most closely related species is WAL 10418 (=KCTC 5947, 97.22 % 16S rRNA gene sequence similarity). Read More

View Article and Full-Text PDF

Pseudoxanthomonas beigongshangi sp. nov., a novel bacteria with predicted nitrite and nitrate reduce ability isolated from pit mud of Baijiu.

Antonie Van Leeuwenhoek 2021 Jun 11. Epub 2021 Jun 11.

Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing, 100048, China.

A Gram-negative and rod-shape bacterium designated REN9, was isolated from pit mud of Baijiu in Sichuan, China. The 16S rRNA sequence of strain REN9 had a high similarity to Pseudoxanthomonas indica P15 (99.21%), P. Read More

View Article and Full-Text PDF

Citrobacter arsenatis sp. nov., an arsenate-reducing bacterium isolated from freshwater sediment.

Antonie Van Leeuwenhoek 2021 Jun 11. Epub 2021 Jun 11.

School of Environmental Science and Engineering, Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation, Hubei Polytechnic University, Huangshi, China.

A novel arsenate-reducing bacterium, LY-1, was isolated from freshwater sediment in Huangshi, China. Morphological analysis indicated that the cells were shaped like rods and were gram-negative. The major fatty acids (> 10%) were C, summed feature 3 (C ω7c, C ω6c) and summed feature 8 (C ω7c, C ω6c). Read More

View Article and Full-Text PDF

Genome sequence of the epiphytic bacteria strain 19_A, isolated from the marine macroalga .

Biotechnol Rep (Amst) 2021 Jun 23;30:e00634. Epub 2021 May 23.

Unidad de Investigaciones Agropecuarias (UNIDIA), Departamento de Microbiología, Pontificia Universidad Javeriana, Bogotá, Colombia.

Microorganisms living on marine macroalgal surfaces require enzyme repertoires to metabolize macroalgal-synthesized compounds. These enzymes are biological catalysts which have specific functional properties for biotechnological applications. Here, we raise awareness on the set of enzyme categories produced by the strain 19_A, isolated from the marine macroalga as revealed by the analysis of its complete genome sequence. Read More

View Article and Full-Text PDF

Direct Comparison of Three Buckling-Based Methods to Measure the Elastic Modulus of Nanobiocomposite Thin Films.

ACS Appl Mater Interfaces 2021 Jun 10. Epub 2021 Jun 10.

Department of Chemistry and Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada.

To engineer tunable thin-film materials, the accurate measurement of their mechanical properties is crucial. However, characterizing the elastic modulus with current methods is particularly challenging for sub-micrometer thick films and hygroscopic materials because they are highly sensitive to environmental conditions and most methods require free-standing films which are difficult to prepare. In this work, we directly compared three buckling-based methods to determine the elastic moduli of supported thin films: (1) biaxial thermal shrinking, (2) uniaxial thermal shrinking, and (3) the mechanically compressed, strain-induced elastic buckling instability for mechanical measurements (SIEBIMM) method. Read More

View Article and Full-Text PDF

Whole-Genome Sequencing of Micrococcus luteus MT1691313, Isolated from the Mariana Trench.

Microbiol Resour Announc 2021 Jun 10;10(23):e0036921. Epub 2021 Jun 10.

School of Ocean, Yantai University, Yantai, China.

Micrococcus luteus MT1691313 is a Gram-positive bacterium isolated from the deep-sea sediment located at a 4,448-m depth in the Mariana Trench. Here, we report the complete genome sequence of this strain, which has a genome size of 2.32 Mb with a GC content of 72. Read More

View Article and Full-Text PDF

sp. nov., an endophytic actinomycete isolated from the root of (L.).

Int J Syst Evol Microbiol 2021 Jun;71(6)

Actinobacterial Research Unit, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.

An endophytic actinobacterium, designated strain CA1R205, was isolated from the surface-sterilized root of L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. Read More

View Article and Full-Text PDF

sp. nov., a novel actinobacterium isolated from the root of pumpkin ().

Int J Syst Evol Microbiol 2021 Jun;71(6)

Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.

A novel acidophilic actinobacterium, designated strain NEAU-YB345, was isolated from a pumpkin root collected from Mudanjiang, Heilongjiang Province, northeast PR China. Based on 16S rRNA gene sequence similarity and chemotaxonomic and morphological properties, the isolate was assigned to the genus , with the high 16S rRNA gene sequence similarities to JCM 16224 (99.2 %), JCM 16223 (99. Read More

View Article and Full-Text PDF

3D printed architected conducting polymer hydrogels.

J Mater Chem B 2021 Jun 8. Epub 2021 Jun 8.

Department of Materials Science and Engineering, University of California, Merced, USA. and Department of Chemistry and Chemical Biology, University of California, Merced, USA.

Conducting polymer hydrogels combine electrical conductivity and tunable water content, rendering them strong candidates for a range of applications including biosensors, cell culture platforms, and energy storage devices. However, these hydrogels are mechanically brittle and prone to damage, prohibiting their use in emerging applications involving dynamic movement and large mechanical deformation. Here, we demonstrate that applying the concept of architecture to conducting polymer hydrogels can circumvent these impediments. Read More

View Article and Full-Text PDF

sp. nov., isolated from rhizosphere soil of .

Int J Syst Evol Microbiol 2021 Jun;71(6)

Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.

A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621, was isolated from rhizosphere soil of in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach. GY 10621 was positive for catalase and oxidase. Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5. Read More

View Article and Full-Text PDF

Complete genome sequence of subsp. strain LLRS-1, isolated from wilted tobacco in China.

Phytopathology 2021 Jun 8. Epub 2021 Jun 8.

Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China;

Here, we present the complete genome sequence and annotation of subsp. strain LLRS-1, which caused bacterial wilt on flue-cured tobacco in Yunnan province, southwest China. Strain LLRS-1 is the first strain identified to be pathogenic to tobacco in China. Read More

View Article and Full-Text PDF

gen. nov., sp. nov. (Chroococcales, Cyanobacteria), isolated from paddy fields in Iran.

Int J Syst Evol Microbiol 2021 Jun;71(6)

Department of Life Sciences, Centre for Functional Ecology, University of Coimbra, Coimbra 3000-456, Portugal.

In Iran, polyphasic studies of unicellular cyanobacteria are still scarce, with more emphasis being placed on filamentous cyanobacteria in paddy fields and fresh water regions. In an effort to increase the knowledge of the diversity of unicellular cyanobacteria from paddy fields in Iran, we have isolated and characterized a new unicellular cyanobacterium strain. The strain was studied using a polyphasic approach based on morphological, ecological and phylogenetic analyses of the 16S-23S ITS rRNA gene region. Read More

View Article and Full-Text PDF

sp. nov. isolated from refrigerated dry-aged beef.

Int J Syst Evol Microbiol 2021 Jun;71(6)

Department of Safety and Quality of Meat, Max Rubner-Institut, Federal Research Institute for Nutrition and Food, E.-C.-Baumann Straße 20, D-95326 Kulmbach, Germany.

A polyphasic approach was applied to investigate the diversity of microbiota that evolved during cold storage beef ripening. Isolate V4/DAB/S4/2a with a unique BOX-rep-PCR fingerprint profile revealed more than 99 % nucleotide identities upon pairwise comparisons of 16S rDNA sequences from the type strains DSM 101070, DSM 108989, DSM 26521 and DSM 29166, placing it within the / branch of the genus . Additional based comparison revealed DSM 101070 as the nearest relative, with 98. Read More

View Article and Full-Text PDF

β-lactam-induced cell envelope adaptations, not solely enhanced daptomycin binding, underlies daptomycin-β-lactam synergy in methicillin-resistant .

Antimicrob Agents Chemother 2021 Jun 7:AAC0035621. Epub 2021 Jun 7.

School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.

Methicillin-resistant () is a serious clinical threat due to innate virulence properties, high infection rates and the ability to develop resistance to multiple antibiotics, including the lipopeptide, daptomycin (). Acquisition of DAP resistance () in MRSA has been linked with several characteristic alterations in the cell envelope. Clinical treatment of DAP-R MRSA infections has generally involved DAP plus β-lactam combinations, although definable synergy of such combinations varies in a strain-dependent, as well as a β-lactam-dependent manner. Read More

View Article and Full-Text PDF

Swi6B, an alternative splicing isoform of Swi6, mediates the cell wall integrity of Ganoderma lucidum.

Environ Microbiol 2021 Jun 7. Epub 2021 Jun 7.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.

The cell wall integrity (CWI) signaling activates the transcription factor Swi6 through a MAPK signaling cascade in response to cell wall stresses. In this study, we observed two different mRNA variants of swi6 (GlSwi6A and GlSwi6B) existed, due to alternative splicing. Besides, the expression level of GlSwi6B was higher than that of the GlSwi6A mRNA variant. Read More

View Article and Full-Text PDF

Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO] Show an Impaired C and N Metabolism When Grown at Ambient [CO].

Front Plant Sci 2021 20;12:656961. Epub 2021 May 20.

Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, United States.

Soybean ( L.) future response to elevated [CO] has been shown to differ when inoculated with strains isolated at ambient or elevated [CO]. Plants, inoculated with three strains isolated at different [CO], were grown in chambers at current and elevated [CO] (400 vs. Read More

View Article and Full-Text PDF

Complete Genome Sequence of and Comparative Analysis of Virulence Gene Content With .

Front Microbiol 2021 21;12:684092. Epub 2021 May 21.

Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.

The bacterial genus contains plant pathogens that are major threats to agriculture in America and Europe. Although extensive research was conducted to characterize different subspecies of (), comparative analysis at above-species levels was lacking due to the unavailability of appropriate data sets. Recently, a bacterium that causes pear leaf scorch (PLS) in Taiwan was described as the second species (i. Read More

View Article and Full-Text PDF

sp. nov., a Nitrogen-Fixing Bacterium Isolated From Forest Soil by Using Optimized Culture Method.

Front Microbiol 2021 21;12:647132. Epub 2021 May 21.

Department of Life Sciences, College of Natural Sciences, Kyonggi University, Suwon-si, South Korea.

A bacterial strain, designated strain G-1-1-14, was isolated from Kyonggi University forest soil during a study of previously uncultured bacterium. The cells of strain G-1-1-14 were motile by means of peritrichous flagella, Gram-stain-negative, rod-shaped, and able to grow autotrophically with hydrogen and fix nitrogen. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain G-1-1-14 belonged to the genus . Read More

View Article and Full-Text PDF

Cold-shock gene cspC in the genome of Massilia polaris sp. nov. revealed cold-adaptation.

Antonie Van Leeuwenhoek 2021 Jun 6. Epub 2021 Jun 6.

Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do, 16227, Republic of Korea.

A straw coloured, motile and Gram-stain-negative bacterium, designated RP-1-19 was isolated from soil of Arctic station, Svalbard, Norway. Based on the phylogenetic analysis of its 16S rRNA gene sequence, strain RP-1-19 formed a lineage within the family Oxalobacteraceae and clustered together within the genus Massilia. The closest members were M. Read More

View Article and Full-Text PDF

Fatty acid composition of the methylotrophic yeast Komagataella phaffii grown under low- and high-methanol conditions.

Yeast 2021 Jun 5. Epub 2021 Jun 5.

The Graduate School of Natural Sciences and Technologies, Gifu University, Gifu, Japan.

In this study, we analysed the intracellular fatty acid profiles of Komagataella phaffii during methylotrophic growth. K. phaffii grown on methanol had significantly lower total fatty acid contents in the cells compared with glucose-grown cells. Read More

View Article and Full-Text PDF

Complete genome of pv. strain A7 isolated from southern Chile.

Mol Plant Microbe Interact 2021 Jun 4. Epub 2021 Jun 4.

Agroadvance SpA, Santiago , Chile;

The complete genome sequence of pv. A7 was obtained by a hybrid approach combining Pacbio and Illumina HiSeq sequencing data. A single circular chromosome of 5. Read More

View Article and Full-Text PDF

Characterization of allelochemicals from Pistia stratiotes extracts and their effects on the growth and physiology of Microcystis aeruginosa.

Environ Sci Pollut Res Int 2021 Jun 4. Epub 2021 Jun 4.

Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.

Due to the public and environmental health impact of cyanotoxins, investigations have been focused on finding environmental friendly algaecides from aquatic plants. The present study had the objective to evaluate the population control and physiological response of Microcystis aeruginosa (Kützing) Kützing (strain BCCUSP232) exposed to Pistia stratiotes L. extracts. Read More

View Article and Full-Text PDF

Fluviispira sanaruensis sp., nov., Isolated from a Brackish Lake in Hamamatsu, Japan.

Curr Microbiol 2021 Jun 4. Epub 2021 Jun 4.

Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, Shizuoka, 432-8561, Japan.

Strain RF1110005, which was isolated from brackish lake water sampled at Lake Sanaru in Japan as a "filterable" bacterial strain, was characterized as a novel species in the genus Fluviispira, family Silvanigrellaceae, order Silvanigrellales, the class Oligoflexia and the phylum Bdellovibrionota. Cells of RF1110005 were aerobic, Gram stain negative, and show a pleomorphic morphology of spiral, filamentous and rod shapes. Catalase reaction was positive. Read More

View Article and Full-Text PDF

Flavobacterium agrisoli sp. nov., a novel bacterium isolated from soil.

Arch Microbiol 2021 Jun 3. Epub 2021 Jun 3.

School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.

A Gram-stain negative, rod shaped, motile by gliding, yellow-pigmented, aerobic bacterium, designated SE-1-e, was isolated from a soil sample collected on Dokdo Island, South Korea. The isolate was characterized taxonomically using a polyphasic approach based on the phenotypic and genomic analyses. The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SE-1-e belonged to the genus Flavobacterium in the family Flavobacteriaceae and had the highest sequence similarity with Flavobacterium cheongpyeongense IMCC34759 (97. Read More

View Article and Full-Text PDF