17,453 results match your criteria contamination chromatography

Formation of a protein corona on the surface of extracellular vesicles in blood plasma.

J Extracell Vesicles 2021 Sep;10(11):e12140

Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.

In this study we tested whether a protein corona is formed around extracellular vesicles (EVs) in blood plasma. We isolated medium-sized nascent EVs of THP1 cells as well as of Optiprep-purified platelets, and incubated them in EV-depleted blood plasma from healthy subjects and from patients with rheumatoid arthritis. EVs were subjected to differential centrifugation, size exclusion chromatography, or density gradient ultracentrifugation followed by mass spectrometry. Read More

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September 2021

Occurrence and distribution of endocrine disrupting chemicals and pharmaceuticals in the river Bouregreg (Rabat, Morocco).

Chemosphere 2021 Sep 8;287(Pt 2):132202. Epub 2021 Sep 8.

Department of Physical and Analytical Chemistry, E.P.S of Linares, University of Jaén, 23700, Linares, Jaén, Spain. Electronic address:

In this work, Moroccan surface waters were analysed for 27 endocrine disrupting chemicals and pharmaceutically active compounds. The study area was selected on the grounds of the scarcity of information about the contamination status of rivers in the Rabat region, which receive micropollutants from municipal and industrial wastewater, and runoff from agricultural fields. In fact, animal feed residues, urban water runoff and untreated waste discharges into old landfills reach river water in an area with a population of ca. Read More

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September 2021

Metabolomic profiles in relation to benchmark polycyclic aromatic compounds (PACs) and trace elements in two seabird species from Arctic Canada.

Environ Res 2021 Sep 7:112022. Epub 2021 Sep 7.

Science and Technology Branch, Environment and Climate Change Canada, Ottawa, ON, Canada. Electronic address:

While exposure of birds to oil-related contaminants has been documented, the related adverse effects this exposure has on Arctic marine birds remain unexplored. Metabolomics can play an important role to explore biologically relevant metabolite biomarkers in relation to different stressors, even at benchmark levels of contamination. The aim of this study was to characterize the metabolomics profiles in relation to polycyclic aromatic compounds (PACs) and trace elements in the liver of two seabird species in the Canadian Arctic. Read More

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September 2021

[Preparation of multi-functional magnetic nanoparticles for harvesting low-molecular-weight glycoproteins].

Se Pu 2021 Oct;39(10):1102-1110

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Low-molecular-weight glycoproteins (LMW-GPs) are considered promising candidates for disease biomarker discovery. Selective sorbents are essential for the extraction and enrichment of this class of compounds. Boronate affinity chromatography is a unique separation mode in liquid chromatography. Read More

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October 2021

Heterologous production of hyaluronic acid in Nicotiana tabacum hairy roots expressing a human hyaluronan synthase 2.

Sci Rep 2021 Sep 9;11(1):17966. Epub 2021 Sep 9.

Institute of Biotechnology, Shiraz University, Shiraz, Iran.

Hyaluronic acid (HA), a unique polysaccharide with excellent Physico-chemical properties, is broadly used in pharmaceutical, biomedical, and cosmetic fields. It is widely present in all vertebrates, certain bacterial strains, and even viruses while it is not found in plants, fungi, and insects. HA is naturally synthesized by a class of integral membrane proteins called Hyaluronic acid synthase (HAS). Read More

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September 2021

Fate of sulfamethoxazole in compost, manure and soil amended with previously stored organic wastes.

Sci Total Environ 2021 Aug 30;803:150023. Epub 2021 Aug 30.

Université Paris-Saclay, INRAE, AgroParisTech, UMR ECOSYS, 78850 Thiverval-Grignon, France.

Application of organic wastes as soil fertilizers represents an important route of agricultural soil contamination by antibiotics such as sulfamethoxazole (SMX). Soil contamination may be influenced by the storage time of organic wastes before soil spreading. The objective of this work was to study the fate of SMX in two organic wastes, a co-compost of green waste and sewage sludge and a bovine manure, which were stored between 0 and 28 days, then incorporated in an agricultural soil that has never received organic waste and monitored for 28 days under laboratory conditions. Read More

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1 An HPLC-MS/MS Method Using a Multitoxin Clean-up Column for Analysis of Seven Mycotoxins in Aquafeeds.

J AOAC Int 2021 Sep 8. Epub 2021 Sep 8.

Hebei Province Key Laboratory of Environment and Human Health, Hebei Medical University, Shijiazhuang, 050017, China.

Background: In Guangdong Province of China, the climate here is very wet, so there are many different fungus living in the aquatic feeds, which produce mycotoxins. These compounds contaminate agriculture products world-wide and represent a great threat to human health. It is necessary to determine their contamination level in aquatic feeds. Read More

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September 2021

Organochlorine pesticide residues in plants and their possible ecotoxicological and agri food impacts.

Sci Rep 2021 Sep 8;11(1):17841. Epub 2021 Sep 8.

Hydrogeochemistry Group, CSIR - National Geophysical Research Institute (NGRI), Uppal Road, Hyderabad, 500007, Telangana, India.

Scientific investigations on levels of Organochlorine Pesticide (OCP) residues in plants largely consider the edible parts (crops, vegetables, and fruit plants). Though the non-edible parts of plants are not eaten by human beings directly, these parts are consumed by livestock and other animals, thereby facilitating the flow of chemical residues through the food chain. The objective of the present investigation was to evaluate the concentration of OCP residues in non-edible plant parts to provide insights on their potential ecotoxicological impacts. Read More

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September 2021

Effect of gamma irradiation on the caffeoylquinic acid derivatives content, antioxidant activity, and microbial contamination of leaves.

Heliyon 2021 Aug 19;7(8):e07825. Epub 2021 Aug 19.

Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, 16424, West Java, Indonesia.

(L.) Less. leaf has a long history of being used as a food and in traditional medicines. Read More

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Screening and elucidation of fragmentations of 23 diuretics in dietary supplements using UHPLC-Q-Orbitrap.

Sci Justice 2021 Sep 3;61(5):451-458. Epub 2021 Jun 3.

Center of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety Osongseangmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28159, Republic of Korea. Electronic address:

Diuretics are used to treat the edematous state in cases of renal insufficiency, nephrotic syndrome, liver cirrhosis, and heart failure. These compounds are used by athletes to lose weight and are included in the list of prohibited substances by the World Anti-Doping Agency. They are also used by obese and overweight people for losing weight, and there are a number of recent reports on the contamination of dietary supplements with diuretics. Read More

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September 2021

Multiple pesticides occurrence, fate, and environmental risk assessment in a small horticultural stream of Argentina.

Sci Total Environ 2021 Aug 28;802:149893. Epub 2021 Aug 28.

Centro de Investigaciones del Medioambiente (CIM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. Electronic address:

Peri-urban horticulture is crucial to local populations, but a global paucity of information exists regarding the contamination of the associated waterways because of this activity. The aim of this study was to assess pesticide pollution of surface water, suspended particulate matter and bottom sediments from the Carnaval Creek Basin (La Plata, Buenos Aires, Argentina) - a representative system of waterways surrounded by horticultural production - by over 40 selected herbicides, insecticides, and fungicides by gas-chromatography-time-of-flight mass spectrometry and ultraperformance liquid chromatography tandem mass spectrometry. Six sampling campaigns were conducted biannually from 2015 to 2017. Read More

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Analysis of cannabis seizures by non-targeted liquid chromatography-tandem mass spectrometry.

J Pharm Biomed Anal 2021 Aug 8;205:114313. Epub 2021 Aug 8.

Institute of Legal Medicine and Core Facility Metabolomics, Medical University of Innsbruck, Mullerstrasse 44, 6020, Innsbruck, Austria. Electronic address:

Due to the popularity of recreational cannabis use, contamination of this drug with diverse classes of chemicals, including pesticides, mycotoxins, and synthetic cannabinoids, has been identified as major threat for public health. For the detection of these compounds in seized cannabis, a screening workflow involving non-targeted liquid chromatography-tandem mass spectrometry (LCMS/MS) was developed. A Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method was used for the extraction of small bioorganic molecules from ground dried material. Read More

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Column-based Technology for CD9-HPLC Immunoaffinity Isolation of Serum Extracellular Vesicles.

J Proteome Res 2021 Sep 2. Epub 2021 Sep 2.

Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109, United States.

Serum-derived extracellular vesicles (EVs) are a promising source of biomarkers; however, major challenges in EV separation and proteomic profiling remain for isolating EVs from a small amount, that is, on the microliter scale, of human serum while minimizing the contamination of blood proteins and lipoprotein particles coeluting in EV preparations. Herein we have developed a column-based CD9-antibody-immobilized high-performance liquid chromatography immunoaffinity chromatography(CD9-HPLC-IAC) technology for EV isolation from a microliter scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8. Read More

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September 2021

Surface enhanced Raman scattering of extracellular vesicles for cancer diagnostics despite isolation dependent lipoprotein contamination.

Nanoscale 2021 Sep 2. Epub 2021 Sep 2.

Department of Biomedical Engineering, University of California, Davis, Davis, CA 95616, USA.

Given the emerging diagnostic utility of extracellular vesicles (EVs), it is important to account for non-EV contaminants. Lipoprotein present in EV-enriched isolates may inflate particle counts and decrease sensitivity to biomarkers of interest, skewing chemical analyses and perpetuating downstream issues in labeling or functional analysis. Using label free surface enhanced Raman scattering (SERS), we confirm that three common EV isolation methods (differential ultracentrifugation, density gradient ultracentrifugation, and size exclusion chromatography) yield variable lipoprotein content. Read More

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September 2021

A sensitive microextraction by packed sorbent-gas chromatography-mass spectrometry method for the assessment of polycyclic aromatic hydrocarbons contamination in Antarctic surface snow.

Chemosphere 2021 Nov 4;282:131082. Epub 2021 Jun 4.

University of Parma, Department of Chemistry, Life Sciences and Environmental Sustainability, Parco Area Delle Scienze 17/A, 43124 Parma, Italy. Electronic address:

For the first time an eco-friendly method involving microextraction by packed sorbent (MEPS) coupled to gas chromatography-mass spectrometry (GC-MS) was developed for the determination of the 16 US-EPA priority pollutant polycyclic aromatic hydrocarbons (PAHs) as indicators of anthropogenic contamination in snow samples collected in polar regions. MEPS was carried out by using C8 sorbent material packed in a barrel insert and needle (BIN) and integrated in the eVol® semi-automatic device. For optimization purposes a Face Centred Design and the multicriteria method of the desirability functions were performed to investigate the effect of some parameters affecting the MEPS extraction efficiency, i. Read More

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November 2021

A modified density gradient proteomic-based method to analyze endolysosomal proteins in cardiac tissue.

iScience 2021 Sep 4;24(9):102949. Epub 2021 Aug 4.

University of Oxford, Department of Pharmacology, Oxford, OX1 3QT UK.

The importance of lysosomes in cardiac physiology and pathology is well established, and evidence for roles in calcium signaling is emerging. We describe a label-free proteomics method suitable for small cardiac tissue biopsies based on density-separated fractionation, which allows study of endolysosomal (EL) proteins. Density gradient fractions corresponding to tissue lysate; sarcoplasmic reticulum (SR), mitochondria (Mito) (1. Read More

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September 2021

A Review on Biosensors and Nanosensors Application in Agroecosystems.

Nanoscale Res Lett 2021 Aug 30;16(1):136. Epub 2021 Aug 30.

Wolaita Sodo University, P.O. Box: 138, Wolaita, Ethiopia.

Previous decades have witnessed a lot of challenges that have provoked a dire need of ensuring global food security. The process of augmenting food production has made the agricultural ecosystems to face a lot of challenges like the persistence of residual particles of different pesticides, accretion of heavy metals, and contamination with toxic elemental particles which have negatively influenced the agricultural environment. The entry of such toxic elements into the human body via agricultural products engenders numerous health effects such as nerve and bone marrow disorders, metabolic disorders, infertility, disruption of biological functions at the cellular level, and respiratory and immunological diseases. Read More

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A Dilute-and-Shoot UHPLC-MS/MS Isotope Dilution Method for Simultaneous Determination and Confirmation of Eleven Mycotoxins in Dried Distillers Grains with Solubles.

J AOAC Int 2021 Aug 30. Epub 2021 Aug 30.

U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Rd, Laurel, Maryland 20708.

Background: Natural contamination of mycotoxins in dried distiller's grains with solubles (DDGS) as a mainstream animal feed ingredient poses risk to animal health.

Objective: A regulatory method was needed for the agency to simultaneously detect eleven mycotoxins of high regulatory priority in DDGS.

Methods: Ten grams of DDGS sample were extracted twice with acetonitrile/water under mildly acidic condition. Read More

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Genetic fingerprinting and aflatoxin production of Aspergillus section Flavi associated with groundnut in eastern Ethiopia.

BMC Microbiol 2021 Aug 28;21(1):239. Epub 2021 Aug 28.

United States Department of Agriculture-Agricultural Research Service-National Peanut Research Laboratory, Dawson, GA, 39842-0509, USA.

Background: Aspergillus species cause aflatoxin contamination in groundnut kernels, being a health threat in agricultural products and leading to commodity rejection by domestic and international markets. Presence of Aspergillus flavus and A. parasiticus colonizing groundnut in eastern Ethiopia, as well as presence of aflatoxins have been reported, though in this region, no genetic studies have been done of these species in relation to their aflatoxin production. Read More

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Irrigation of soil with reclaimed wastewater acts as a buffer of microbial taxonomic and functional biodiversity.

Sci Total Environ 2021 Aug 14;802:149671. Epub 2021 Aug 14.

Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal. Electronic address:

The usage of reclaimed wastewater (RWW) for irrigation of agricultural soils is increasingly being acknowledged for reducing water consumption by promoting reuse of treated wastewater, and for the delivery of extant nutrients in the soil. The downside is that RWW may be a vector for contamination of soils with contaminants of emerging concern (CECs), if left uncontrolled. Its usage is anticipated to alter the soil properties, consequently also the soil microbial community. Read More

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An Improved LC-MS/MS Method for the Analysis of Thirteen Cytostatics on Workplace Surfaces.

Pharmaceuticals (Basel) 2021 Jul 31;14(8). Epub 2021 Jul 31.

LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal.

Cytostatics are drugs used in cancer treatment, which pose serious risks to healthcare workers. Dermal absorption via surface contamination is the key exposure route; thus, rapid, reliable, and validated analytical methods for multicomponent detection are crucial to identify the exposure risk. A surface-wipe-sampling technique compatible with hospitals' safety requirements (gauze, 1 mL isopropanol) and a fast and simple extraction method (1 mL acetonitrile, 20 min ultrasonic bath, evaporation, reconstitution in 200 µL acetonitrile), coupled with liquid chromatography-tandem mass spectrometry analysis, were developed. Read More

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Occurrence of pyrethroids in the atmosphere of urban areas of Southeastern Brazil: Inhalation exposure and health risk assessment.

Environ Pollut 2021 Aug 20;290:118020. Epub 2021 Aug 20.

Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Laboratório de Micropoluentes Jan Japenga, Av. Carlos Chagas Filho, 373, 21941-902, Rio de Janeiro, RJ, Brazil; Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Laboratório de Radioisótopos Eduardo Penna Franca, Av. Carlos Chagas Filho, 373, 21941-902, Rio de Janeiro, RJ, Brazil.

The occurrence of organochlorine pesticides (OCPs) used decades ago for vector control in urban areas is still reported as a threat to human health. Pyrethroids emerged as a replacement for OCPs in sanitary campaigns and are currently the main insecticides used for vector control worldwide, with prominent use as agricultural and household insecticides, for veterinary and gardening purposes, and as wood preservative. This study aimed to assess the occurrence, seasonal variation, and potential sources of pyrethroids in ambient air of two urban regions of Southeastern Brazil, along with the potential health risks to local populations via inhalation exposure. Read More

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Identification of Tomato Infecting Viruses That Co-Isolate with Nanovesicles Using a Combined Proteomics and Electron-Microscopic Approach.

Nanomaterials (Basel) 2021 Jul 26;11(8). Epub 2021 Jul 26.

Extracellular Vesicles and Mass Spectrometry Laboratory, Institute of Biosciences and BioResources, National Research Council of Italy, 80131 Naples, Italy.

Plant-derived nanovesicles (NVs) have attracted interest due to their anti-inflammatory, anticancer and antioxidative properties and their efficient uptake by human intestinal epithelial cells. Previously we showed that tomato ( L.) fruit is one of the interesting plant resources from which NVs can be obtained at a high yield. Read More

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Design of Nanostructured Lipid Carriers Containing (Palmarosa) Essential Oil against .

Molecules 2021 Aug 10;26(16). Epub 2021 Aug 10.

Department of Health Basic Sciences, State University of Maringá, Maringá 87020-900, PR, Brazil.

Palmarosa essential oil (PEO) is an alternative to synthetic fungicides to control the contamination by food-deteriorating fungi, such as . Nonetheless, the low long-term stability and volatility hamper its utilization. Thus, this study aimed to develop nanostructured lipid carriers (NLCs) containing PEO to improve its stability and consequently prolong the activity against . Read More

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Pyrrolizidine Alkaloids in the Food Chain: Is Horizontal Transfer of Natural Products of Relevance?

Foods 2021 Aug 7;10(8). Epub 2021 Aug 7.

Institute of Pharmaceutical Biology, Technical University of Braunschweig, Mendelssohnstr. 1, 38106 Braunschweig, Germany.

Recent studies have raised the question whether there is a potential threat by a horizontal transfer of toxic plant constituents such as pyrrolizidine alkaloids (PAs) between donor-PA-plants and acceptor non-PA-plants. This topic raised concerns about food and feed safety in the recent years. The purpose of the study described here was to investigate and evaluate horizontal transfer of PAs between donor and acceptor-plants by conducting a series of field trials using the PA-plant as model and realistic agricultural conditions. Read More

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In Vitro Antibacterial Effect of the Methanolic Extract of the Korean Soybean Fermented Product Doenjang against .

Animals (Basel) 2021 Aug 5;11(8). Epub 2021 Aug 5.

Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, 165 00 Prague, Czech Republic.

Ultra-high performance liquid chromatography/mass spectrometry showed soyasaponin I and the isoflavones daidzein, genistein, and glycitein to be the main components of the methanolic extract of the Korean soybean fermented product doenjang, which is known to be a rich source of naturally occurring bioactive substances, at average contents of 515.40, 236.30, 131. Read More

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Study of the occurrence of toxic alkaloids in forage grass by liquid chromatography tandem mass spectrometry.

J Chromatogr A 2021 Sep 12;1654:462463. Epub 2021 Aug 12.

Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China; Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, P.R. China. Electronic address:

The toxic alkaloids in forage grass present a serious health hazard to humans and livestock, especially ergot alkaloids (EAs), pyrrolizidine alkaloids (PAs) and tropane alkaloids (TAs). Hence, there is a need for a simultaneous method that allows these dangerous plant toxins to be determined. A simple and effective method was developed to determine fifteen toxic alkaloids (EAs, PAs and TAs) in forage grass using the QuEChERS method and liquid chromatography tandem mass spectrometry (LC-MS/MS). Read More

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September 2021

Changes of Milk Metabolomic Profiles Resulting from a Mycotoxins-Contaminated Corn Silage Intake by Dairy Cows.

Metabolites 2021 Jul 23;11(8). Epub 2021 Jul 23.

Department of Animal Science, Food and Nutrition, Faculty of Agricultural, Food and Environmental Sciences, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy.

In this study, an untargeted metabolomics approach based on ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) was used for investigating changes in chemical profiles of cow milk considering diets based on mycotoxins-contaminated corn silages. For this purpose, 45 milk samples were classified into five clusters according to the corn silage contamination profile, namely (1) low levels of - and -mycotoxins; (2) low levels of fumonisins and other -mycotoxins; (3) high levels of -mycotoxins; (4) high levels of non-regulated -mycotoxins; (5) high levels of fumonisins and their metabolites, and subsequently analyzed by UHPLC-HRMS followed by a multivariate statistical analysis (both unsupervised and supervised statistical approaches). Overall, the milk metabolomic profile highlighted potential correlations between the quality of contaminated corn silages (as part of the total mixed ration) and milk composition. Read More

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Innovation in Strategies for Sensitivity Improvement of Chromatography and Mass Spectrometry Based Analytical Techniques.

Crit Rev Anal Chem 2021 Aug 26:1-17. Epub 2021 Aug 26.

National Institute of Pharmaceutical Education and Research-Ahmedabad (NIPER-A), An Institute of National Importance, Government of India, Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, Gandhinagar, Gujarat, India.

Chromatography and mass spectrometry based techniques are the most commonly employed procedures to quantitate the analytes in pharmaceutical research. However, sensitivity of analytical methods significantly varies due to the difference in physicochemical characteristics of analytes. Sensitivity of methods greatly affects the quality of analytical results. Read More

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Optimization of Crude Oil Biodegradation by sp. Isolated from the Native Sponges of the Persian Gulf.

Iran J Biotechnol 2021 Apr 1;19(2):e2690. Epub 2021 Apr 1.

Department of Environmental Science, Persian Gulf Research Institute, Persian Gulf University, Bushehr, Iran.

Background: The native sponges of Persian Gulf are unique species facing difficult climate conditions and environmental contamination. It is necessary to investigate these native sponges because global warming most probably destroyed many of these creatures. Therefore, the study of the microorganisms associated with sponges will introduce new bacterial strains with various industrial and environmental applications and, in this way, a part of the Persian Gulf biodiversity will be preserved for posterity. Read More

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