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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component.

Vet Comp Oncol 2021 May 7. Epub 2021 May 7.

University of Nottingham, BioDiscovery Institute, Nottingham, UK.

A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n=41). Read More

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The barley SHN1-type transcription factor HvSHN1 imparts heat, drought and salt tolerances in transgenic tobacco.

Plant Physiol Biochem 2021 May 1;164:44-53. Epub 2021 May 1.

Laboratory of Plant Biotechnology and Improvement, University of Sfax, Center of Biotechnology of Sfax, Route Sidi Mansour, Km 6 B.P' 1177, 3018 Sfax, Tunisia. Electronic address:

The APETAL2/Ethylene Responsive Factor (AP2/ERF) family was the subject of intensive research which led to the identification of several members involved in different stress responses such as salinity, drought and high temperature. The SHN/WIN clade of AP2/ERF participates in many important processes such as cutin and wax biosynthesis, ethylene signaling and gene expression. Here, we report the functional analysis of SHN1-type transcription factor, HvSHN1, from barely. Read More

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Gamma sterilization of collagen/hydroxyapatite composites: Validation and radiation effects.

Appl Radiat Isot 2021 May 1;174:109758. Epub 2021 May 1.

Universidade Federal de Minas Gerais, Escola de Engenharia, Departamento de Engenharia Metalúrgica e de Materiais, Bloco 2, Sala 2233, Avenida Presidente Antônio Carlos 6627, Pampulha, Belo Horizonte, Minas Gerais, CEP, 31270-901, Brazil. Electronic address:

In this work, gamma sterilization was validated, and the impact of this sterilization process on collagen/hydroxyapatite (Col/HAp) composites was investigated. It has been already recognized that the improper sterilization of healthcare products may lead to infection and mortality/morbidity issues in patients. Gamma sterilization has emerged as a promising sterilization method because it shows advantages such as low cost, a small increase in temperature of irradiated materials, and no production of toxic residues. Read More

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Insights into the acquisition of the island and production of colibactin in the population.

Microb Genom 2021 May;7(5)

IRSD, INSERM, Université de Toulouse, INRAE, ENVT, UPS, Toulouse, France.

The island codes for the enzymes necessary for synthesis of the genotoxin colibactin, which contributes to the virulence of strains and is suspected of promoting colorectal cancer. From a collection of 785 human and bovine isolates, we identified 109 strains carrying a highly conserved island, mostly from phylogroup B2, but also from phylogroups A, B1 and D. Different scenarios of acquisition were deduced from whole genome sequence and phylogenetic analysis. Read More

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Specific neuronal subpopulations in the rat basolateral amygdala express high levels of non-phosphorylated neurofilaments.

J Comp Neurol 2021 May 7. Epub 2021 May 7.

Department of Pharmacology, Physiology and Neuroscience, University of South Carolina School of Medicine, Columbia, South Carolina, United States.

Cortical pyramidal neurons (PNs) containing non-phosphorylated neurofilaments (NNFs) localized with the SMI-32 monoclonal antibody have been shown to be especially vulnerable to degeneration in Alzheimer's disease (AD). The present investigation is the first to study the expression of SMI-32+ NNFs in neurons of the basolateral nuclear complex of the amygdala (BNC), which contains cortex-like PNs and nonpyramidal neurons (NPNs). We observed that PNs in the rat basolateral nucleus (BL), but not in the lateral (LAT) or basomedial (BM) nuclei, have significant levels of SMI-32-ir in their somata with antibody diluents that did not contain Triton X-100, but staining in these cells was greatly attenuated when the antibody diluent contained 0. Read More

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Impacts of effluent from different livestock farm types (pig, cow, and poultry) on surrounding water quality: a comprehensive assessment using individual parameter evaluation method and water quality indices.

Environ Sci Pollut Res Int 2021 May 6. Epub 2021 May 6.

Department of Environmental Management, Faculty of Environment, Vietnam National University of Agriculture, Hanoi, Vietnam.

Water pollution within and nearby different livestock farm types was assessed comprehensively for the first time in Vietnam. The samples of wastewater, ground water, and surface water were collected from 130 pig farms, 80 poultry farms, and 40 cow farms. Water quality was first assessed by individual parameter evaluation method in which measured values of water quality parameters were compared with the permissible limits in the national technical regulations on livestock's effluent (QCVN 62), surface water quality, and ground water quality. Read More

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A combination of Lactobacillus buchneri and Pediococcus pentosaceus extended the aerobic stability of conventional and BMR-corn hybrids ensiled at low dry matter concentrations by causing a major shift in their bacterial and fungal community.

J Anim Sci 2021 May 7. Epub 2021 May 7.

Department of Animal Science, North Carolina State University, Raleigh, North Carolina, USA.

We evaluated the effects of applying a combination inoculant to 4 corn hybrids harvested at high moisture on their nutritive value and microbial populations. The treatment design was the factorial combination of corn hybrids ensiled with (INO) and without (CON) inoculant. The hybrids were TMF2R737 (MCN), F2F817 (MBR), P2089YHR (PCN), and PI144XR (PBR), ensiled at dry matter (DM) concentrations of 30. Read More

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Molecular Typing of Community-acquired Methicillin-Resistant Isolated from 2- to 6-year old Children by Staphylococcal Protein A and Typing in Isfahan, Iran.

Adv Biomed Res 2021 27;10. Epub 2021 Jan 27.

Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Methicillin-resistant (MRSA) has become a considerable public health concern in the entire world due to the rapid spread of this bacterium in human community; also the epidemiology of MRSA has changed, as the isolation of MRSA strains from healthy and non-healthy patients. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of community-acquired (CA)-MRSA nasal carriage in the Iranian samples.

Materials And Methods: A total of 25 CA-MRSA were isolated from the anterior nares of 410 healthy preschool children. Read More

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January 2021

Integrating Network Pharmacology and Experimental Validation to Decipher the Mechanism of Action of Huanglian Jiedu Decoction in Treating Atherosclerosis.

Drug Des Devel Ther 2021 30;15:1779-1795. Epub 2021 Apr 30.

The Department of Cardiovascular Disease, Guangzhou University of Traditional Chinese Medicine First Affiliated Hospital, Guangzhou, Guangdong, People's Republic of China.

Background: This study used network pharmacology, molecular docking and experimental validation to assess the effects of Huanglian Jiedu Decoction (HLJDD) on atherosclerosis (AS).

Methods: The components and targets of HLJDD were analyzed using the Traditional Chinese Medicine Systems Pharmacology database, and information on the genes associated with AS was retrieved from the GeneCards and OMIM platforms. Protein-protein interactions were analyzed using the STRING platform. Read More

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Community surveillance of COVID-19 by village health volunteers, Thailand.

Bull World Health Organ 2021 May 2;99(5):393-397. Epub 2021 Mar 2.

Graduate School of Health Innovation, Kanagawa University of Human Services, 3-25-10 Tonomchi, Kawasaki, Kanagawa 210-0821, Japan.

Problem: To control the increasing spread of coronavirus disease 2019 (COVID-19), the government of Thailand enforced the closure of public and business areas in Bangkok on 22 March 2020. As a result, large numbers of unemployed workers returned to their hometowns during April 2020, increasing the risk of spreading the virus across the entire country.

Approach: In anticipation of the large-scale movement of unemployed workers, the Thai government trained existing village health volunteers to recognize the symptoms of COVID-19 and educate members of their communities. Read More

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Evaluating the measures taken to contain a Candida auris outbreak in a tertiary care hospital in South India: an outbreak investigational study.

BMC Infect Dis 2021 May 6;21(1):425. Epub 2021 May 6.

Department of General Medicine and Division of Infectious Diseases, Amrita Institute of Medical Sciences, Kochi, Kerala, India.

Background: Candida auris infections are an emerging global threat with poor clinical outcome, high mortality rate, high transmission rate and outbreak potential. The objective of this work is to describe a multidisciplinary approach towards the investigation and containment of a Candida auris outbreak and the preventive measures adopted in a resource limited setting.

Methods: This outbreak investigational study was conducted at a 1300-bedded tertiary care academic hospital in South India. Read More

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Long-read metagenomics retrieves complete single-contig bacterial genomes from canine feces.

BMC Genomics 2021 May 6;22(1):330. Epub 2021 May 6.

Molecular Genetics Veterinary Service (SVGM), Veterinary School, Universitat Autònoma de Barcelona, Barcelona, Spain.

Background: Long-read sequencing in metagenomics facilitates the assembly of complete genomes out of complex microbial communities. These genomes include essential biologic information such as the ribosomal genes or the mobile genetic elements, which are usually missed with short-reads. We applied long-read metagenomics with Nanopore sequencing to retrieve high-quality metagenome-assembled genomes (HQ MAGs) from a dog fecal sample. Read More

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Microbial and chemical pollutants on the manure-crops pathway in the perspective of "One Health" holistic approach.

Sci Total Environ 2021 Apr 29;785:147411. Epub 2021 Apr 29.

The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-100 Gliwice, Poland; Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland.

This study determined the impact of poultry and bovine manure fertilization on the content of antibiotics, heavy metals (HMs), and the quantitative and qualitative composition of integrase and antibiotic resistance genes in soil, groundwater, and crops cultivated on manure-amended plots. Antibiotic concentration levels were analyzed using the HPLC-MS/MS, heavy metal concentration level were measured by HGAAS and ICP-OES, while the integrase genes and ARGs were quantified using Real-Time PCR (qPCR) method. Manure, soil, and crops samples contained the highest concentration of Zn (10-10 ng g) and Cu (10-10 ng g) of all HMs tested. Read More

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Non-invasive diagnosis of colorectal cancer by Raman spectroscopy: Recent developments in liquid biopsy and endoscopy approaches.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 16;258:119818. Epub 2021 Apr 16.

Raman Project Center for Medical and Biological Applications, Shimane University, Matsue, Japan; Faculty of Life and Environmental Sciences, Shimane University, Matsue, Japan. Electronic address:

Colorectal cancer (CRC) is the third most common cancer diagnosed globally and is also one of the leading causes of cancer deaths in both men and women. The progression of CRC is slow and is often contained in colon but the risk increases with age. Based on the high certainty that the net benefit of screening in an age group is substantial, screening for CRC is recommended beginning at the age of 50. Read More

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A simplified cluster model and a tool adapted for collaborative labeling of lung cancer CT scans.

Comput Methods Programs Biomed 2021 Apr 18;206:106111. Epub 2021 Apr 18.

Research and Practical Clinical Center for Diagnostics and Telemedicine Technologies of the Moscow Health Care Department, Petrovka str., 24, Moscow, 127051, Russia; Federal Research Center "Computer Science and Control" of Russian Academy of Sciences, Vavilova str., 44/2, Moscow, 119333, Russia. Electronic address:

Background And Objective: Lung cancer is the most common type of cancer with a high mortality rate. Early detection using medical imaging is critically important for the long-term survival of the patients. Computer-aided diagnosis (CAD) tools can potentially reduce the number of incorrect interpretations of medical image data by radiologists. Read More

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Analysis of Microbiological and Chemical Hazards in Edible Insects Available to Canadian Consumers.

J Food Prot 2021 May 6. Epub 2021 May 6.

Canadian Food Inspection Agency.

Edible insects are a novel food in most countries; their popularity is growing because of their high protein/low fat content, ease of cultivation, and small environmental impact. To our knowledge, this is the first report that addresses both microbiological and chemical hazards in edible insects. Samples were collected from retail stores or purchase through e-commerce. Read More

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Detection of Species, and -Like and -Like Endosymbionts in and from a Shared Field Site in Georgia, United States of America.

Vector Borne Zoonotic Dis 2021 May 5. Epub 2021 May 5.

Rickettsial Zoonoses Branch, United States Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Two abundant species of aggressive ticks commonly feed on humans in Georgia: the Gulf Coast tick () and the Lone Star tick (). is the primary host of , " Rickettsia andeanae," and a -like endosymbiont (AmacFLE), whereas is the primary host for , , , and a -like endosymbiont (AamCLE). Horizontal transmission of from to by co-feeding has been described, and has been found infrequently in ticks. Read More

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Reporting guidelines for artificial intelligence in healthcare research.

Clin Exp Ophthalmol 2021 May 6. Epub 2021 May 6.

Academic Unit of Ophthalmology, Institute of Inflammation and Ageing, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.

Reporting guidelines are structured tools developed using explicit methodology that specify the minimum information required by researchers when reporting a study. The use of AI reporting guidelines that address potential sources of bias specific to studies involving AI interventions has the potential to improve the quality of AI studies, through improvements in their design and delivery, and the completeness and transparency of their reporting. With a number of guidance documents relating to AI studies emerging from different specialist societies, this Review article provides researchers with some key principles for selecting the most appropriate reporting guidelines for a study involving an AI intervention. Read More

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Identification and quantification of plasticizers, bisphenol, and environmental toxic mineral elements residues in medicines from Tunisian markets.

Environ Sci Pollut Res Int 2021 May 6. Epub 2021 May 6.

Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, University of Messina, Messina, Italy.

In this study, 18 plasticizer (phthalates, adipates, sebacates, and others) residues in sixteen medicines available in Tunisian pharmaceutical markets are evaluated by MSPD combined with GC-MS. In parallel, in this research, UPLC-MS/MS technique was performed to detect bisphenol A, and cadmium, lead, chromium, cobalt, nickel, mercury, selenium, and arsenic levels were determined by ICP-MS. The maximum daily ingested mass of mineral elements was calculated and compared with permissible daily exposure limits published by USP NF 2013. Read More

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The Predation Game: Does dividing attention affect patterns of human foraging?

Cogn Res Princ Implic 2021 May 6;6(1):35. Epub 2021 May 6.

Faculty of Psychology, School of Health Sciences, University of Iceland, Oddi v. Sturlugötu, 101, Reykjavik, Iceland.

Attention is known to play an important role in shaping the behaviour of both human and animal foragers. Here, in three experiments, we built on previous interactive tasks to create an online foraging game for studying divided attention in human participants exposed to the (simulated) risk of predation. Participants used a "sheep" icon to collect items from different target categories randomly distributed across the display. Read More

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[Corrigendum] High PLK4 expression promotes tumor progression and induces epithelial‑mesenchymal transition by regulating the Wnt/β‑catenin signaling pathway in colorectal cancer.

Int J Oncol 2021 Jun 6;58(6). Epub 2021 May 6.

Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.

Subsequently to the publication of the above article, the author realized that Fig. 5 on p. 486 contained some errors on account of the figure having been compiled incorrectly; essentially, the published version of the figure contained incorrect images for the panels presented in Fig. Read More

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MALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening.

Rapid Commun Mass Spectrom 2021 May 5:e9121. Epub 2021 May 5.

Proteome Center Rostock, Medical Faculty and Natural Science Faculty, University of Rostock, Schillingallee 69, 18057, Rostock, Germany.

Rationale: To open new ways for MALDI-MS-based patient screening, blood serum is the most preferred specimen because of its richness in patho-physiological information and due to ease of collection. To overcome deleterious freeze/thaw cycles and to reduce high costs for shipping and storage, we sought to develop a procedure which enables MALDI-MS protein profiling of blood serum proteins without the need for serum freezing.

Methods: Blood sera from patients/donors were divided into portions which after pre-incubation were fast frozen. Read More

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Apolipoprotein B Is Associated With the Microenvironment of Cholangiocarcinoma.

Front Oncol 2021 31;11:654689. Epub 2021 Mar 31.

Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Cholangiocarcinoma (CCA) is a kind of devastating malignancy, which is correlated with the extremely high mortality. Due to the occult pathogenesis of CCA, most patients are diagnosed in the advanced stage. However, the efficacy of chemotherapy and immunotherapy is limited for these patients. Read More

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Bioinformatics analysis of laryngeal squamous cell carcinoma: seeking key candidate genes and pathways.

PeerJ 2021 14;9:e11259. Epub 2021 Apr 14.

Department of Otolaryngology, Cangzhou Central Hospital, Cangzhou, Hebei, China.

Background: Laryngeal squamous cell carcinoma (LSCC) is the second most aggressive head and neck squamous cell carcinoma. Although much work has been done to optimize its treatment, patients with LSCC still have poor prognosis. Therefore, figuring out differentially expressed genes (DEGs) contained in the progression of LSCC and employing them as potential therapeutic targets or biomarkers for LSCC is extremely meaningful. Read More

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Giving women WOICE postpartum: prevalence of maternal morbidity in high-risk pregnancies using the WHO-WOICE instrument.

BMC Pregnancy Childbirth 2021 May 5;21(1):357. Epub 2021 May 5.

Department of Obstetrics and Gynecology, School of Medicine, University of Campinas, Rua Alexander Fleming 101, Campinas, São Paulo, 13083-891, Brazil.

Background: There are no accurate estimates of the prevalence of non-severe maternal morbidities. Given the lack of instruments to fully assess these morbidities, the World Health Organization (WHO) developed an instrument called WOICE. We aimed to evaluate the prevalence of non-severe maternal morbidities in puerperal women and factors associated to impaired clinical, social and mental health conditions. Read More

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Psychoanalysis with adults inspired by parent-infant therapy: Reconstructing infantile trauma.

Int J Psychoanal 2020 Apr;101(2):320-339

Department of Women's and Children's Health, Karolinska Institute, Stockholm, Sweden.

This the first in a series of articles on how Psychodynamic Therapy with Infants and Parents (PTIP) can inspire work with adult therapy. PTIP helps infants and parents improve their relationship and facilitate child development. During sessions, developmental hazards are dramatized by parent and baby, giving the therapist first-hand impressions of how conflictual relationships impact on the well-being of mother and child. Read More

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A highly virulent canine distemper virus strain isolated from vaccinated mink in China.

Virus Genes 2021 May 5. Epub 2021 May 5.

National Research Center for Veterinary Medicine, No.3 Cuiwei Road, High-Tech District, Luoyang, Henan, 471003, PR China.

An outbreak of canine distemper in 2017 in mink breeding farms (Shandong province, China) caused severe pneumonia, hardened footpads, and death in more than 5000 vaccinated animals. Sequencing of the hemagglutinin and fusion protein genes from the WH2 canine distemper virus (CDV) strain we isolated from the infected minks were clustered into the recently isolated CDV Asia-1 genotype group. The WH2 strain was distinct from the current vaccine strains, containing a novel potential N-glycosylation site in its hemagglutinin protein. Read More

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Construction of a high-nuclearity Nd(III) nanoring for the NIR luminescent detection of antibiotics.

Dalton Trans 2021 May;50(17):5865-5870

The University of Texas at Austin, Department of Chemistry and Biochemistry, 1 University Station A5300, Austin, Texas 78712, USA.

One NIR luminescent 14-metal Nd(iii) nanoring (1, molecular size: 1.0 × 2.2 × 2. Read More

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Establishment and verification of prognostic model for gastric cancer based on autophagy-related genes.

Am J Cancer Res 2021 15;11(4):1335-1346. Epub 2021 Apr 15.

Department of Gastroenterology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer Tianjin 300060, P. R. China.

Autophagy played a significant role in the development of cancer. In this study, we explored the value of autophagy-associated genes in gastric cancer. RNA sequencing and clinical information containing 375 gastric cancer and 32 normal tissues were gathered from the TCGA portal. Read More

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Integration of non-communicable disease and HIV/AIDS management: a review of healthcare policies and plans in East Africa.

BMJ Glob Health 2021 May;6(5)

Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, UK

Background: Low-income and middle-income countries are struggling to manage growing numbers of patients with chronic non-communicable diseases (NCDs), while services for patients with HIV infection are well established. There have been calls for integration of HIV and NCD services to increase efficiency and improve coverage of NCD care, although evidence of effectiveness remains unclear. In this review, we assess the extent to which National HIV and NCD policies in East Africa reflect the calls for HIV-NCD service integration. Read More

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