1,559 results match your criteria contact osteogenesis

Osteoinduction Evaluation of Fluorinated Hydroxyapatite and Tantalum Composite Coatings on Magnesium Alloys.

Front Chem 2021 7;9:727356. Epub 2021 Sep 7.

Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC and Academic Center for Dentistry Amsterdam (ACTA), Amsterdam Movement Science (AMS), Vrije Universiteit Amsterdam (VU), Amsterdam, Netherlands.

Magnesium (Mg) alloys have a wide range of biomaterial applications, but their lack of biocompatibility and osteoinduction property impedes osteointegration. In order to enhance the bioactivity of Mg alloy, a composite coating of fluorinated hydroxyapatite (FHA) and tantalum (Ta) was first developed on the surface of the alloy through thermal synthesis and magnetron sputtering technologies in this study. The samples were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive spectroscopy (EDS) mapping, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and water contact angle measurement (WCA), which characterized the surface alternation and confirmed the deposition of the target FHA/Ta coating. Read More

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September 2021

[Surface modification of titanium implant with hBMP-2/hIGF-1 for promoting biocompatibility and osteogenesis].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Aug;41(8):1277-1282

Department of Implantology, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China.

Objective: To prepare the human bone morphogenetic protein-2(hBMP-2)/human insulin-like growth factor-1(hIGF-1)coating titanium(Ti)and assess its performance as a dental implant material.

Methods: hBMP-2 and hIGF-1 were coated to the smooth surface of a Ti plate, and its efficacy for promoting bone formation and bone integration was compared with a pristine Ti plate.The surface characteristics of the metal samples were evaluated using scanning electron microscope (SEM) and by contact angle measurement. Read More

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Study on the effect of surface characteristics of short-pulse laser patterned titanium alloy on cell proliferation and osteogenic differentiation.

Mater Sci Eng C Mater Biol Appl 2021 Sep 30;128:112349. Epub 2021 Jul 30.

Institute of Artificial Intelligence, Donghua University, Shanghai 201620, China. Electronic address:

Concise, low-cost preparation of titanium alloy implants with high cell proliferation and osteogenic differentiation is urgently needed. Nanosecond laser ablation of titanium alloy has the advantages of short processing time, less pollution, and non-contact. In this research, we adopt a nanosecond UV laser to process the closed groove and cross groove titanium alloys with length to width ratio of 1:1, 2. Read More

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September 2021

Hydrothermally treated titanium surfaces for enhanced osteogenic differentiation of adipose derived stem cells.

Mater Sci Eng C Mater Biol Appl 2021 Sep 14;128:112315. Epub 2021 Jul 14.

Department of Mechanical Engineering, Colorado State University, Fort Collins, CO 80523, USA; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO 80523, USA. Electronic address:

Implant surface plays a crucial role in improving osseointegration and long-term implant life. When the implant comes in contact with the bone tissue, the bone marrow mesenchymal cells interact with the implant surface and the surface properties such as morphology, wettability, mechanical properties and chemistry influences cell migration, proliferation and differentiation. Different surface modification strategies such as ceramic coatings, surface dealloying, and surface topography modifications for improving osteointegration have been investigated. Read More

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September 2021

Direct-Deposited Graphene Oxide on Dental Implants for Antimicrobial Activities and Osteogenesis.

Int J Nanomedicine 2021 24;16:5745-5754. Epub 2021 Aug 24.

Department of Prosthodontics, School of Dentistry, Chonnam National University, Gwangju, Korea.

Objective: To determine the effects of graphene oxide (GO) deposition (on a zirconia surface) on bacterial adhesion and osteoblast activation.

Methods: An atmospheric pressure plasma generator (PGS-300) was used to coat Ar/CH mixed gas onto zirconia specimens (15-mm diameter × 2.5-mm thick disks) at a rate of 10 L/min and 240 V. Read More

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Coating Polyelectrolyte Multilayers Loaded with Quercetin on Titanium Surfaces by Layer-By-Layer Assembly Technique to Improve Surface Osteogenesis Under Osteoporotic Condition.

J Biomed Nanotechnol 2021 Jul;17(7):1392-1403

Department of Orthopaedics Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Lucheng District, Wenzhou 325000, Zhejiang Province, People's Republic of China.

Titanium (Ti) and its alloy implants are widely used in the field of orthopedics, and osteoporosis is an important reason for implantation failure. This study aimed to establish a quercetin (QTN) controlled release system on the surface of titanium implants and to study its effects on osteogenesis and osseointegration on the surface of implants. Polyethylenimine (PEI) was first immobilized on a titanium substrate as the base layer, and then, hyaluronic acid/chitosan-quercetin (HA/CS-QTN) multilayer films were assembled on the PEI layer by a self-assembly technique. Read More

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CSBD Healing in Rats after Application of Bovine Xenogeneic Biomaterial Enriched with Magnesium Alloy.

Int J Mol Sci 2021 Aug 23;22(16). Epub 2021 Aug 23.

Department of Anatomy, Medical Faculty, University of Rijeka, 51 000 Rijeka, Croatia.

Xenogeneic biomaterials Cerbone and OsteoBiol are widely used in oral implantology. In dental practice, xenogeneic biomaterial is usually combined with autologous bone to provide bone volume stability needed for long-term dental implants. Magnesium alloy implants dissolve and form mineral corrosion layer that is directly in contact with bone tissue, allowing deposition of the newly formed bone. Read More

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PGS/HAp Microporous Composite Scaffold Obtained in the TIPS-TCL-SL Method: An Innovation for Bone Tissue Engineering.

Int J Mol Sci 2021 Aug 10;22(16). Epub 2021 Aug 10.

Department of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology (WUST), Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland.

In this research, we synthesize and characterize poly(glycerol sebacate) pre-polymer (pPGS) (H NMR, FTiR, GPC, and TGA). Nano-hydroxyapatite (HAp) is synthesized using the wet precipitation method. Next, the materials are used to prepare a PGS-based composite with a 25 wt. Read More

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Efficacy of platelet-rich fibrin on bone formation, part 2: Guided bone regeneration, sinus elevation and implant therapy.

Int J Oral Implantol (Berl) 2021 08;14(3):285-302

Purpose: To investigate the effect of platelet-rich fibrin on bone formation by investigating its use in guided bone regeneration, sinus elevation and implant therapy.

Materials And Methods: This systematic review and meta-analysis were conducted and reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The eligibility criteria comprised human controlled clinical trials comparing the clinical outcomes of platelet-rich fibrin with those of other treatment modalities. Read More

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Zeolite Socony Mobil-Five Coating on Ti-24 Nb-4 Zr-7.9 Sn Promotes Biocompatibility and Osteogenesis In Vitro and In Vivo.

Biomed Res Int 2021 29;2021:5529368. Epub 2021 Jul 29.

Department of Prosthodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang 110002, China.

The aim of this study was to evaluate the biocompatibility and osteogenic potential of a Zeolite Socony Mobil-5 (ZSM-5) coating on a Ti-24 Nb-4 Zr-7.9 Sn (Ti-2448) surface. ZSM-5-modified Ti-2448 (ZSM-5/Ti-2448) and Ti-2448 (control) groups were employed. Read More

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September 2021

Semaphorin3A promotes osseointegration of titanium implants in osteoporotic rabbits.

Clin Oral Investig 2021 Aug 6. Epub 2021 Aug 6.

Department of Stomatology, Nanjing Integrated Traditional Chinese and Western Medicine Hospital, No.179, Xiaolingwei Road, Xuanwu District, Nanjing, 210014, Jiangsu, China.

Objective: In the present study, we intend to assess the function of Sema3A in osteointegration of titanium implants both in vivo and in vitro.

Material And Methods: Briefly, Sema3A was transfected in HBMSCs cells to detect its effect on osteogenesis. Subsequently, an in vivo rabbit model was established. Read More

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Effect of Argon-Based Atmospheric Pressure Plasma Treatment on Hard Tissue Formation on Titanium Surface.

Int J Mol Sci 2021 Jul 16;22(14). Epub 2021 Jul 16.

Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, Japan.

In this paper, we suggest that the atmospheric pressure plasma treatment of pure titanium metal may be useful for improving the ability of rat bone marrow cells (RBMCs) to induce hard tissue differentiation. Previous studies have reported that the use of argon gas induces a higher degree of hard tissue formation. Therefore, this study compares the effects of plasma treatment with argon gas on the initial adhesion ability and hard tissue differentiation-inducing ability of RBMCs. Read More

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Nano/micro implant debris affect osteogenesis by chondrocytes: Comparison between ceramic and UHMWPE from hip walking simulator.

J Biomed Mater Res B Appl Biomater 2021 Jul 21. Epub 2021 Jul 21.

University of Carthage, National Institute of Applied Sciences and Technology, UR17ES22, Tunis, Tunisia.

A new generation of ceramic on ceramic (BIOLOX ®delta) bearings has emerged more than 10 years ago proving a high resistance to wear and good clinical results. However, biological reactions to wear debris, particularly the nanoparticles, need to be evaluated. The first originality of this study is to start from real wear particles obtained by the hip walking simulator (CERsim). Read More

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3D-Printed Submicron Patterns Reveal the Interrelation between Cell Adhesion, Cell Mechanics, and Osteogenesis.

ACS Appl Mater Interfaces 2021 Jul 12;13(29):33767-33781. Epub 2021 Jul 12.

Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology (TU Delft), Mekelweg 2, 2628 CD Delft, The Netherlands.

The surface topography of implantable devices is of crucial importance for guiding the cascade of events that starts from the initial contact of the cells with the surface and continues until the complete integration of the device in its immediate environment. There is, however, limited quantitative information available regarding the relationships between the different stages of such cascade(s) and how the design of surface topography influences them. We, therefore, used direct laser writing to 3D-print submicron pillars with precisely controlled dimensions and spatial arrangements to perform a systematic study of such relationships. Read More

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Sclerostin-Mediated Impaired Osteogenesis by Fibroblast-Like Synoviocytes in the Particle-Induced Osteolysis Model.

Front Mol Biosci 2021 23;8:666295. Epub 2021 Jun 23.

Institute for Skeletal Aging and Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon, South Korea.

Engineered biomaterials are envisioned to replace, augment, or interact with living tissues for improving the functional deformities associated with end-stage joint pathologies. Unfortunately, wear debris from implant interfaces is the major factor leading to periprosthetic osteolysis. Fibroblast-like synoviocytes (FLSs) populate the intimal lining of the synovium and are in direct contact with wear debris. Read More

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Multifunctional polyplex micelles for efficient microRNA delivery and accelerated osteogenesis.

Nanoscale 2021 Jul 7;13(28):12198-12211. Epub 2021 Jul 7.

Department of Biomedical Sciences, Texas A&M University College of Dentistry, Dallas, TX 75246, USA.

MicroRNAs (miRNAs) are emerging as a novel class of molecular targets and therapeutics to control gene expression for tissue repair and regeneration. However, a safe and effective transfection of miRNAs to cells has been a major barrier to their applications. In this work, a multifunctional polyplex micelle named PPP-RGI was developed as a non-viral gene vector for the efficient transfection of miR-218 (an osteogenic miRNA regulator) to bone marrow-derived mesenchymal stem cells (BMSCs) for accelerated osteogenic differentiation. Read More

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Magnetron sputtering of strontium nanolayer on zirconia implant to enhance osteogenesis.

Mater Sci Eng C Mater Biol Appl 2021 Aug 19;127:112191. Epub 2021 May 19.

Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC and Academic Center for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam (VU), Amsterdam Movement Science (AMS), Amsterdam, the Netherlands; Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), Amsterdam, the Netherlands.

The zirconia implants have a wide range of clinical applications, however, the biological inertness and lack of osteoinductive properties limit these applications. Strontium possesses superior biocompatibility and excellent osteogenic properties. To take advantage of these, the strontium titanate-coated zirconia implants were prepared in this study by sandblasting, acid etching, and magnetron sputtering, followed by the analysis of the biological behavior. Read More

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Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application.

Int J Nanomedicine 2021 25;16:4321-4332. Epub 2021 Jun 25.

Department of Stomatology, Binzhou Medical University Hospital, Binzhou, 256603, People's Republic of China.

Background: As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.

Purpose: A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.

Methods: In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release. Read More

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Polydopamine-assisted immobilization of silk fibroin and its derived peptide on chemically oxidized titanium to enhance biological activity in vitro.

Int J Biol Macromol 2021 Aug 29;185:1022-1035. Epub 2021 Jun 29.

Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan, Shanxi, China. Electronic address:

Biochemical modification can endow the surface of implants with superior biological activity. Herein, silk fibroin (SF) protein and its anionic derivative peptides (Cs) were covalently immobilized onto a titanium implant surface via a polydopamine layer. The successful conjugation of SF and Cs was revealed by X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), and contact angle measurements. Read More

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Bone screws of porous silicon carbide coated with tantalum improve osseointegration and osteogenesis in goat femoral neck fractures.

Biomed Mater 2021 Aug 18;16(5). Epub 2021 Aug 18.

Orthopaedic of Department, Affiliated ZhongShan Hospital of Dalian University, Dalian 116001, People's Republic of China.

Traditional metal materials, such as stainless steel and titanium (Ti) alloys, are still the gold standards for fracture fixation. However, the elastic moduli of these materials differ from that of human cortical bone, and the stress shielding effect affects fracture healing, leading to secondary fractures. Herein, a new porous Ta coated SiC (pTa-SiC) scaffold using in internal fixation devices with good mechanical and biological properties was prepared based on porous silicon carbide (SiC) scaffold and tantalum (Ta) metal. Read More

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De novo bone formation around implants with a surface based on a monolayer of multi-phosphonate molecules. An experimental in vivo investigation.

Clin Oral Implants Res 2021 Sep 5;32(9):1085-1096. Epub 2021 Jul 5.

Postgraduate Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.

Objectives: The purpose of this experimental in vivo investigation was to evaluate the influence of modifying the implant surface by adding a monolayer of multi-phosphonate molecules on the de novo bone formation and osseointegration.

Material And Methods: The study was designed as an animal preclinical trial with intra-animal control and two healing periods, 2 and 8 weeks, to compare implants with an identical macro-design but with two different surfaces. Eight female Beagle dogs participated in the study. Read More

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September 2021

BMP2 immune complexes promote new bone formation by facilitating the direct contact between osteoclasts and osteoblasts.

Biomaterials 2021 08 16;275:120890. Epub 2021 May 16.

Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, 401147, China; Department of Oral Implantology, Stomatological Hospital of Chongqing Medical University, Chongqing, 401147, China. Electronic address:

BMP2 antibody is proposed as a promising replacement for rhBMP2 in bone tissue engineering. Although studies have demonstrated its osteoinductive efficacy, the underlying osteogenic mechanism and adverse reactions of specific BMP2 antibody are not clarified yet, making it difficult to optimize the antibody for future application. By establishing BMP2 immune complexes (BMP2-ICs) ex vivo, we were able to introduce BMP2-ICs directly in vivo and found that BMP2-ICs promoted bone formation while suppressing osteoclastogenesis. Read More

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In vivo evaluation of deer antler trabecular bone as a reconstruction material for bone defects.

Res Vet Sci 2021 Sep 11;138:116-124. Epub 2021 Jun 11.

Department of Clinical Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, Quartier Vallée 2, Avenue de Cureghem 1 - B67, Liège, Belgium.

Availability of graft materials to fill up osseous defects has always been a concern in orthopaedic surgeries. Deer antler material is a primary bone structure that is easy to collect and could serve as a xenograft. This study examines the behaviour of red deer antler trabecular cylinders in critical size distal femoral epiphyseal defects in 11 rabbits, and evaluates the effect of the decellularization protocols. Read More

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September 2021

A stochastic micro to macro mechanical model for the evolution of bone-implant interface stiffness.

Acta Biomater 2021 09 12;131:415-423. Epub 2021 Jun 12.

Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.

Upon placement of an implant into living bone, an interface is formed through which various biochemical, biological, physical, and mechanical interactions take place. This interface evolves over time as the mechanical properties of peri-implant bone increase. Owing to the multifactorial nature of interfacial processes, it is challenging to devise a comprehensive model for predicting the mechanical behavior of the bone-implant interface. Read More

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September 2021

The Osteogenic Assessment of Mineral Trioxide Aggregate-based Endodontic Sealers in an Organotypic Ex Vivo Bone Development Model.

J Endod 2021 Sep 11;47(9):1461-1466. Epub 2021 Jun 11.

BoneLab-Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, Porto, Portugal; Associated Laboratory for Green Chemistry/Network of Chemistry and Technology (LAQV/REQUIMTE), University of Porto, Porto, Portugal.

Introduction: Mineral trioxide aggregate (MTA)-based sealers are endodontic materials with widespread success in distinct clinical applications, potentially embracing direct contact with the bone tissue. Bone response to these materials has been traditionally addressed in vitro. Nonetheless, translational data are limited by the absence of native cell-to-cell and cell-to-matrix interactions that hinder the representativeness of the analysis. Read More

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September 2021

Polyelectrolyte multilayer composite coating on 316 L stainless steel for controlled release of dual growth factors accelerating restoration of bone defects.

Mater Sci Eng C Mater Biol Appl 2021 Jul 14;126:112187. Epub 2021 May 14.

Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei, Taiwan. Electronic address:

A composite coating of polyelectrolyte multilayers (PEMs) consisting of collagen, a chitosan barrier, and poly-γ-glutamic acid was fabricated using a spin coating technique to investigate and overcome the limited osseointegration capacity of 316 L stainless steel (316 L SS). To further enhance the biocompatibility, bone morphogenetic protein 2 (BMP-2) and basic fibroblast growth factor-2 (FGF-2) were loaded separately as dual growth factors, allowing for progressive drug release following the natural process of bone regeneration. The first burst release of FGF-2 triggered the proliferation of surrounding cells, and the subsequent release of BMP-2 stimulated their differentiation. Read More

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Cross-Talk Between Mesenchymal Stromal Cells (MSCs) and Endothelial Progenitor Cells (EPCs) in Bone Regeneration.

Front Cell Dev Biol 2021 13;9:674084. Epub 2021 May 13.

Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium.

Bone regeneration is a complex, well-orchestrated process based on the interactions between osteogenesis and angiogenesis, observed in both physiological and pathological situations. However, specific conditions (e.g. Read More

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The hypertrophic cartilage induction influences the building-block capacity of human adipose stem/stromal cell spheroids for biofabrication.

Artif Organs 2021 Oct 15;45(10):1208-1218. Epub 2021 Jun 15.

Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, Duque de Caxias, Brazil.

As an alternative to the classical tissue engineering approach, bottom-up tissue engineering emerges using building blocks in bioassembly technologies. Spheroids can be used as building blocks to reach a highly complex ordered tissue by their fusion (bioassembly), representing the foundation of biofabrication. In this study, we analyzed the biomechanical properties and the fusion capacity of human adipose stem/stromal cell (ASC) we spheroids during an in vitro model of hypertrophic cartilage established by our research group. Read More

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October 2021

In Vivo and In Vitro Analyses of Titanium-Hydroxyapatite Functionally Graded Material for Dental Implants.

Biomed Res Int 2021 30;2021:8859945. Epub 2021 Apr 30.

The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, China.

Purpose: The stress shielding effect caused due to the mechanical mismatch between the solid titanium and the surrounding bone tissue warrants the utilization of a mechanically and biologically compatible material such as the titanium-hydroxyapatite (Ti-HA) functionally graded material (FGM) for dental implants. This study is aimed at fabricating a Ti-HA FGM with superior mechanical and biological properties for dental implantation.

Materials And Methods: We fabricated a Ti-HA FGM with different Ti volume fractions (VFs) using HA and Ti powders. Read More

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September 2021

Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration.

Int J Nanomedicine 2021 13;16:3329-3342. Epub 2021 May 13.

Department of Oral Implantology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, 510055, People's Republic of China.

Background And Purpose: Micro-/nano-tubes (TNTs) and micro-/nano-nets (TNNs) are the common and sensible choice in the first step of combined modifications of titanium surface for further functionalization in the purpose of extended indications and therapeutic effect. It is important to recognize the respective biologic reactions of these two substrates for guiding a biologically based first-step selection.

Materials And Methods: TNTs were produced by anodic oxidation and TNNs were formed by alkali-heat treatment. Read More

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