4,198 results match your criteria conjunctival epithelial

Oxidative and antioxidative stress markers in dry eye disease: A systematic review and meta-analysis.

Acta Ophthalmol 2021 May 2. Epub 2021 May 2.

Université Clermont Auvergne, CNRS, LaPSCo, Physiological and Psychosocial Stress, CHU Clermont-Ferrand, University Hospital of Clermont-Ferrand, Preventive and Occupational Medicine, Witty Fit, Clermont-Ferrand, France.

Purpose: To conduct a systematic review and meta-analysis on the levels of oxidative stress markers and antioxidants in dry eye disease (DED) compared with healthy subject.

Method: The PubMed, Cochrane Library, Embase, Science Direct and Google Scholar databases were searched on 10 January 2021 for studies reporting oxidative and antioxidative stress markers in DED and healthy controls. Main meta-analysis was stratified by type of biomarkers, type of samples (tears, conjunctival cells or biopsies), Sjögren's syndrome (SS) (patients with or without SS) and by geographical zones (Asia or Europe). Read More

View Article and Full-Text PDF

Clinical outcomes of modified simple limbal epithelial transplantation for limbal stem cell deficiency in Chinese population: a retrospective case series.

Stem Cell Res Ther 2021 May 1;12(1):259. Epub 2021 May 1.

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing Ophthalmology & Visual Sciences Key Laboratory, Capital Medical University, Beijing, 100730, China.

Objective: To report the clinical outcomes of a novel surgical technique, namely simple limbal epithelial transplantation (SLET), for the treatment of limbal stem cell deficiency (LSCD).

Methods: Thirteen patients (13 eyes) with LSCD who underwent autologous (10 eyes) or allogeneic (3 eyes) modified SLET between 2018 and 2021 were enrolled in this study. Grades of symblepharon, corneal conjunctivalization, vascularization, opacification, and visual acuity (VA) were evaluated preoperatively and postoperatively. Read More

View Article and Full-Text PDF

Efficacy of Modified Amnion-Assisted Conjunctival Epithelial Redirection (ACER) for Partial Limbal Stem Cell Deficiency.

Medicina (Kaunas) 2021 Apr 10;57(4). Epub 2021 Apr 10.

Singapore National Eye Centre, Singapore 168751, Singapore.

: the aim of this study was to analyze the efficacy of a modified "amnion-assisted conjunctival epithelial redirection (ACER)" technique for the treatment of partial limbal stem cell deficiency (LSCD). : the medical records of three patients with partial LSCD who underwent corneal surface reconstruction with modified ACER following superficial keratectomy were retrospectively studied. Briefly, in this technique, an inner amniotic membrane (AM) layer was applied on the corneal surface to promote corneal re-epithelialization. Read More

View Article and Full-Text PDF

Treatment of Capecitabine Corneal Side Effects With Autologous Blood-derived Serum Eye Drops.

In Vivo 2021 May-Jun;35(3):1881-1884

Ophthalmic Clinic, Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.

Background/aim: To describe the clinical progress and management of ocular side effects in a 35-year-old patient with metastatic breast cancer who underwent oral chemotherapy with capecitabine and lapatinib.

Materials And Methods: Slit lamp evaluation revealed bilateral perikeratic hyperemia, perilimbal conjunctival edema associated with corneal marginal infiltrates and epithelial and anterior stromal defects in both eyes. Slit lamp examination, in vivo confocal microscopy and anterior-segment optical coherence tomography were highly suggestive for limbal stem cell deficiency. Read More

View Article and Full-Text PDF
February 2021

Gene therapy in the anterior eye segment.

Curr Gene Ther 2021 Apr 22. Epub 2021 Apr 22.

Eye Program, Board of Governors Regenerative Medicine Institute and Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, United States.

This review provides comprehensive information about the advances in gene therapy in the anterior segment of the eye including cornea, conjunctiva, lacrimal gland, and trabecular meshwork. We discuss gene delivery systems including viral and non-viral vectors as well as gene editing techniques, mainly CRISPR-Cas9, and epigenetic treatments including antisense and siRNA therapeutics. We also provide a detailed analysis of various anterior segment diseases where gene therapy has been tested with corresponding outcomes. Read More

View Article and Full-Text PDF

Herpes simplex keratoconjunctivitis in the immediate postoperative period after strabismus surgery.

Strabismus 2021 Apr 26:1-4. Epub 2021 Apr 26.

Ophthalmology Department, Alder Hey Children's Hospital, Alder Hey Children's NHS Foundation Trust, Liverpool.

The authors describe the case of bilateral herpes simplex keratoconjunctivitis (HSK) following uncomplicated 7 mm bilateral lateral rectus recessions in a 3-year-old child. The recovery was initially unremarkable, and the standard postoperative drops of dexamethasone and chloramphenicol (non-preservative free) were prescribed. The child presented 8 days postoperatively with fever, right upper lid swelling and ptosis. Read More

View Article and Full-Text PDF

A single cell atlas of human cornea that defines its development, limbal progenitor cells and their interactions with the immune cells.

Ocul Surf 2021 Apr 16. Epub 2021 Apr 16.

Biosciences Institute, Faculty of Medical Sciences, Newcastle University, UK. Electronic address:

Purpose: Single cell (sc) analyses of key embryonic, fetal and adult stages were performed to generate a comprehensive single cell atlas of all the corneal and adjacent conjunctival cell types from development to adulthood.

Methods: Four human adult and seventeen embryonic and fetal corneas from 10 to 21 post conception week (PCW) specimens were dissociated to single cells and subjected to scRNA- and/or ATAC-Seq using the 10x Genomics platform. These were embedded using Uniform Manifold Approximation and Projection (UMAP) and clustered using Seurat graph-based clustering. Read More

View Article and Full-Text PDF

Cevimeline-induced anti-inflammatory effect through upregulations of mucins in the ocular surface of a dry eye mouse model.

Biomed Pharmacother 2021 Apr 13;139:111571. Epub 2021 Apr 13.

T2B infrastructure Center for Ocular Disease, Inje University Busan Paik Hospital, Busan 47392, Republic of Korea; Department of Ophthalmology, Inje University College of Medicine, Inje University Busan Paik Hospital, Busan 47392, Republic of Korea. Electronic address:

This study aimed to investigate the effects of various concentrations of cevimelines (CVMs) and compare them with commercial drugs in a murine model of dry eye. The experimental mouse model used male and female NOD.B10. Read More

View Article and Full-Text PDF

The role of cyclin D1, BCL-2, p53 and Ki-67 in epithelial cells in the etiopathogenesis of pterygium.

Indian J Pathol Microbiol 2021 Apr-Jun;64(2):238-242

Department of Pathology, Health Sciences University, Antalya Education and Research Hospital, Antalya, Turkey.

Context: Pterygium is a degenerative disease that consists of conjunctival epithelia and fibrovascular tissue. Some studies suggest that there is a defect in the regulation of apoptosis in the epithelial cell cycle characterized by the development of the disease. But, still this matter being debated. Read More

View Article and Full-Text PDF

Unusual Clinical Presentation of Giant Extragenital Condyloma.

Acta Dermatovenerol Croat 2020 Dec;28(7):240-241

Andrija Jovic, MD, Clinical Center of Nis, Clinic of Dermatovenereology , Bulevar Zorana Djindjica 48, Nis, Serbia;

Dear editor, Condylomata accuminatum (CA) is a human papillomavirus (HPV) related sexually transmitted infection (STI), clinically characterized by solitary or even clustered dark red or pink lesions solely affecting the anogenital area (1). CA involving the extragenital, non-mucosal skin has been sporadically reported (2-4). Diagnosis of CA is usually straightforward when the lesions are located on the anogenital area. Read More

View Article and Full-Text PDF
December 2020

Histopathological Variability and Concomitant Lesions in Pterygium in a Large Case Series.

J Ophthalmol 2021 19;2021:6623794. Epub 2021 Mar 19.

The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, McGill University, Montreal, Quebec, Canada.

Pterygium is a common lesion consisting of fleshy conjunctival growth extending towards the cornea. There is no documented risk of malignant transformation; however, concomitant disease is not rare, and its link to sunlight exposure indicates a risk of other malignancies. The purpose of our study is to describe histopathological features of resected pterygiums and to recognize patients at risk of other conjunctival diseases. Read More

View Article and Full-Text PDF

Pembrolizumab-induced Stevens-Johnson Syndrome with Severe Ocular Complications.

Ocul Immunol Inflamm 2021 Apr 7:1-3. Epub 2021 Apr 7.

The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea.

: To report a unique case of Stevens-Johnson syndrome (SJS) with severe ocular complications induced by pembrolizumab, an immune checkpoint inhibitor.: A 64-year-old man with urothelial cancer presented with bilateral corneal epithelial defect and conjunctival pseudo-membrane formation with erythematous patches on the four extremities, abdomen, and back. He had urothelial cancer with multifocal lesions in the renal pelvis and left ureter, and para-aortic metastatic lymph nodes. Read More

View Article and Full-Text PDF

Lid margin keratinization in Stevens-Johnson syndrome: Review of pathophysiology and histopathology.

Ocul Surf 2021 Apr 3. Epub 2021 Apr 3.

Centre for Ocular Regeneration (CORE), L V Prasad Eye Institute, Hyderabad, Telangana, India; Brien Holden Eye Research Centre (BHERC), L V Prasad Eye Institute, Hyderabad, Telangana, India; The Cornea Institute, L V Prasad Eye Institute, Hyderabad, Telangana, India. Electronic address:

Lid margin keratinization (LMK) is a chronic ocular sequela of Stevens-Johnson syndrome (SJS), which causes lid wiper epitheliopathy and progressive ocular surface damage. The exact etiopathogenesis of LMK, however, remains elusive. This review summarizes the potential pathophysiological mechanisms of LMK and describes its histopathological features. Read More

View Article and Full-Text PDF

Developmental Cysts of the Orbit With Mixed Conjunctival and Cutaneous Epithelial Phenotypes.

Ophthalmic Plast Reconstr Surg 2021 Mar 31. Epub 2021 Mar 31.

Orbital Service, Moorfields Eye Hospital, London, United Kingdom Department of Histopathology, Institute of Ophthalmology, London, United Kingdom.

Aim: To describe the characteristics of patients presenting with congenital orbital cysts containing epithelia of both cutaneous phenotype-that is, epidermis with or without keratin, and at least one dermal structure (sweat gland or pilosebaceous unit)-and conjunctival phenotype (with goblet cells); these cysts with mixed lining are termed "dermo-conjunctival" cysts.

Patients And Methods: Review of clinical records for patients having dermo-conjunctival cysts that were treated between 1997 and 2017; patients with cysts of solely conjunctival or solely cutaneous origin were omitted. Data recorded included gender, age at presentation, laterality, orbital location, ophthalmic features, surgical and radiological data, and light microscopic findings. Read More

View Article and Full-Text PDF

The Ultrastructure of the Nictitating Membrane of the Little Penguin (, Aves).

Integr Org Biol 2020 5;2(1):obaa048. Epub 2021 Jan 5.

Department of Optometry and Vision Science, University of New South Wales, Kensington, NSW 2052, Australia.

The ultrastructure of the nictitating membrane in the little penguin was studied using both scanning and transmission electron microscopy to improve our understanding of the function of ocular adnexa in diving birds. Following euthanasia, eyes were enucleated and immersion fixed in Karnovsky's fixative. The nictitating membrane and conjunctiva were embedded in araldite and semi- or ultra-thin sections were stained and photographed using compound and transmission electron microscopes, respectively. Read More

View Article and Full-Text PDF
January 2021

CLEAR - Contact lens complications.

Cont Lens Anterior Eye 2021 Apr 25;44(2):330-367. Epub 2021 Mar 25.

School of Optometry, Aston University, Birmingham, UK.

Contact lens-related complications are common, affecting around one third of wearers, although most are mild and easily managed. Contact lenses have well-defined anatomical and physiological effects on the ocular surface and can result in other consequences due to the presence of a biologically active material. A contact lens interacts with the tear film, ocular surface, skin, endogenous and environmental microorganisms, components of care solutions and other antigens which may result in disease specific to contact lens wear, such as metabolic or hypersensitivity disorders. Read More

View Article and Full-Text PDF

Stratifin in ocular surface squamous neoplasia and its association with p53.

Acta Ophthalmol 2021 Mar 26. Epub 2021 Mar 26.

Ophthalmoplasty Service, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.

Purpose: Sunlight-induced p53 mutations are known to contribute towards increased risk of ocular surface squamous neoplasia (OSSN). Stratifin (14-3-3σ)/HEM (human epithelial marker) is a p53-mediated inhibitor of cell cycle progression and has been shown to be a target of epigenetic deregulation in various carcinomas. In the present study, Stratifin expression, its promoter methylation status as well as expression of mutant p53 in early and advanced AJCC stages (8th edition) of OSSN, was evaluated. Read More

View Article and Full-Text PDF

Process development and safety evaluation of ABCB5 limbal stem cells as advanced-therapy medicinal product to treat limbal stem cell deficiency.

Stem Cell Res Ther 2021 Mar 19;12(1):194. Epub 2021 Mar 19.

TICEBA GmbH, Im Neuenheimer Feld 517, 69120, Heidelberg, Germany.

Background: While therapeutic success of the limbal tissue or cell transplantation to treat severe cases of limbal stem cell (LSC) deficiency (LSCD) strongly depends on the percentage of LSCs within the transplanted cells, prospective LSC enrichment has been hampered by the intranuclear localization of the previously reported LSC marker p63. The recent identification of the ATP-binding cassette transporter ABCB5 as a plasma membrane-spanning marker of LSCs that are capable of restoring the cornea and the development of an antibody directed against an extracellular loop of the ABCB5 molecule stimulated us to develop a novel treatment strategy based on the utilization of in vitro expanded allogeneic ABCB5 LSCs derived from human cadaveric limbal tissue.

Methods: We developed and validated a Good Manufacturing Practice- and European Pharmacopeia-conform production and quality-control process, by which ABCB5 LSCs are derived from human corneal rims, expanded ex vivo, isolated as homogenous cell population, and manufactured as an advanced-therapy medicinal product (ATMP). Read More

View Article and Full-Text PDF

Benefits of autologous platelet tissue graft in wound healing after corneal refractive surgery: a case report.

J Med Case Rep 2021 Mar 16;15(1):122. Epub 2021 Mar 16.

Takayanagi EYE Clinic, Sapporo S1 Building 3F, Nishi4-20-5, Minami1-jo, Chuo-ku, Sapporo, Hokkaido, 060-0807, Japan.

Background: Corneal refractive surgeries cause epithelial damage and induce wound healing processes. To promote wound healing after photorefractive keratectomy, the effectiveness of an autologous platelet tissue graft was assessed.

Case Presentation: A 45-year-old Asian male with low myopia and/or myopic astigmatism received photorefractive keratectomy in both eyes. Read More

View Article and Full-Text PDF

Review of Approaches for Increasing Ophthalmic Bioavailability for Eye Drop Formulations.

AAPS PharmSciTech 2021 Mar 14;22(3):107. Epub 2021 Mar 14.

Chemical and Biological Engineering Department, Colorado School of Mines, Alderson Hall, 1613 Illinois St., Golden, Colorado, 80401, USA.

Ophthalmic diseases represent a significant problem as over 2 billion people worldwide suffer from vison impairment and blindness. Eye drops account for around 90% of ophthalmic medications but are limited in success due to poor patient compliance and low bioavailability. Low bioavailability can be attributed to short retention times in the eye caused by rapid tear turnover and the difficulty of drug diffusion through the multi-layered structure of the eye that includes lipid-rich endothelial and epithelial layers as well as the stroma which is high in water content. Read More

View Article and Full-Text PDF

Multifunctional nanocomposite eye drops of cyclodextrin complex@layered double hydroxides for relay drug delivery to the posterior segment of the eye.

Carbohydr Polym 2021 May 13;260:117800. Epub 2021 Feb 13.

Department of Pharmaceutical, School of Pharmacy, China Pharmaceutical University, 24 Tongjia Xiang, Nanjing, 210009, China. Electronic address:

Topical drug delivery system to the posterior segment of the eye is facing many challenges, such as rapid drug elimination, low permeability, and low concentration at the targeted sites. To overcome these challenges, Multifunctional nanocomposite eye drops of dexamethasone-carboxymethyl-β-cyclodextrin@layered double hydroxides-glycylsarcosine (DEX-CM-β-CD@LDH-GS) were developed for relay drug delivery. Herein, our studies demonstrated that DEX-CM-β-CD@LDH-GS could penetrate through human conjunctival epithelial cells with an intact structure and exhibited integrity in the sclera of rabbits' eyes with in vivo fluorescence resonance energy transfer imaging. Read More

View Article and Full-Text PDF

Non-malignant conjunctival epithelial masses with ocular surface squamous neoplasia-like optical coherence tomography features.

Int Ophthalmol 2021 May 10;41(5):1827-1834. Epub 2021 Mar 10.

Department of Ophthalmology, Semmelweis University, Budapest, Hungary.

Purpose: To observe and describe the anterior segment optical coherence tomography features of limbally localised non-malignant epithelial mass lesions METHODS: Thirteen patients (age: 66.9 ± 16.3 years) with conjunctival mass suggesting ocular surface squamous neoplasia with biomicroscopic examination were imaged using anterior segment ocular coherence tomography (anterior segment optical coherence tomography)/Cirrus HD-OCT, Model 4000, Carl Zeiss Meditec, Inc. Read More

View Article and Full-Text PDF

[Simple limbal epithelial transplantation (SLET) : A simple technique for the treatment of unilateral complete limbal stem cell deficiency. Video article].

Ophthalmologe 2021 Apr 8;118(4):404-412. Epub 2021 Mar 8.

Klinik für Augenheilkunde, Uniklinik RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Deutschland.

Objective: The aim of simple limbal epithelial transplantation (SLET) is the regeneration of the corneal surface in unilateral complete limbal stem cell deficiency (LSCD).

Indications: SLET is indicated for unilateral complete LSCD.

Contraindications: Contraindications include bilateral LSCD, severe corneal thinning, pronounced keratoconjunctivitis sicca, chronic inflammatory condition of the ocular surface, malposition of the eyelids and pronounced adhesions of the conjunctiva with trichiasis. Read More

View Article and Full-Text PDF

The micronucleus cytome assay - A fast tool for DNA damage screening in human conjunctival epithelial cells.

Ocul Surf 2021 Mar 4;20:195-198. Epub 2021 Mar 4.

Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic.

Purpose: To assess whether the micronucleus cytome assay (MCyt) reliably detects DNA damage occurring in control and pathological superficial epithelial cells from human conjunctiva.

Methods: Impression cytology samples from the bulbar conjunctiva of 33 healthy controls, eight patients with conjunctival intraepithelial neoplasia (CIN) and eight with mucous membrane pemphigoid (MMP) were examined using the MCyt modified for the ocular surface.

Results: The mean number of micronuclei (MNi) in control samples was 0. Read More

View Article and Full-Text PDF

Human limbal epithelial stem cell regulation, bioengineering and function.

Prog Retin Eye Res 2021 Mar 4:100956. Epub 2021 Mar 4.

Cornea Division, Stein Eye Institute, University of California, Los Angeles, CA, 90095, USA. Electronic address:

The corneal epithelium is continuously renewed by limbal stem/progenitor cells (LSCs), a cell population harbored in a highly regulated niche located at the limbus. Dysfunction and/or loss of LSCs and their niche cause limbal stem cell deficiency (LSCD), a disease that is marked by invasion of conjunctival epithelium into the cornea and results in failure of epithelial wound healing. Corneal opacity, pain, loss of vision, and blindness are the consequences of LSCD. Read More

View Article and Full-Text PDF

Ocular surface characteristics and colonization in a burn center: A prospective cohort study.

J Burn Care Res 2021 Mar 1. Epub 2021 Mar 1.

Center for Population Health Research. Instituto Nacional de Salud Pública. Cuernavaca, Morelos, Mexico.

We aimed to evaluate the characteristics and colonization by pathogenic microorganisms of the ocular surface in patients in a burn center and to determine their association with sedation, mechanical ventilation, and periocular burn. We prospectively evaluated 40 patients during an eight-month period. Five evaluations where performed, at baseline and weekly on four more occasions or until hospital discharge or death. Read More

View Article and Full-Text PDF

Detection of Autophagy-Related Gene Expression by Conjunctival Impression Cytology in Age-Related Macular Degeneration.

Diagnostics (Basel) 2021 Feb 12;11(2). Epub 2021 Feb 12.

Department of Ophthalmology, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.

Purpose: To investigate the association of autophagy-related gene expression with age-related macular degeneration (AMD).

Methods: Patients with AMD were recruited for analysis by conjunctival impression cytology. mRNA was assessed by real-time polymerase chain reaction (RT-PCR) to evaluate whether the expression of 26 autophagy-related genes (ATGs) was correlated with AMD. Read More

View Article and Full-Text PDF
February 2021

Material Characterisation and Stratification of Conjunctival Epithelial Cells on Electrospun Poly(ε-Caprolactone) Fibres Loaded with Decellularised Tissue Matrices.

Pharmaceutics 2021 Feb 28;13(3). Epub 2021 Feb 28.

Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, L7 8TX, UK.

The conjunctiva, an under-researched yet incredibly important tissue, plays key roles in providing protection to the eye and maintaining homeostasis of its ocular surface. Multiple diseases can impair conjunctival function leading to severe consequences that require surgical intervention. Small conjunctival defects can be repaired relatively easily, but larger defects rely on tissue grafts which generally do not provide adequate healing. Read More

View Article and Full-Text PDF
February 2021

Blue Light Induces Impaired Autophagy through Nucleotide-Binding Oligomerization Domain 2 Activation on the Mouse Ocular Surface.

Int J Mol Sci 2021 Feb 18;22(4). Epub 2021 Feb 18.

Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju 61469, Korea.

In this study, we investigated the effects of blue light exposure on nucleotide-binding oligomerization domain 2 (NOD2) expression on the mouse ocular surface and evaluated the role of NOD2 activation in light-induced cell death. Mice were divided into wild-type (WT), NOD2-knock out (KO), WT + blue light (WT + BL), and NOD2-KO + blue light (NOD2-KO + BL) groups, and the mice in the WT+BL and NOD2-KO + BL groups were exposed to blue light for 10 days. After 10 days of blue light exposure, increased reactive oxygen species and malondialdehyde were observed in the WT + BL and NOD2-KO + BL groups, and the WT + BL group showed a higher expression of NOD2 and autophagy related 16 like 1. Read More

View Article and Full-Text PDF
February 2021

Co-expression of the SARS-CoV-2 entry receptors ACE2 and TMPRSS2 in healthy human conjunctiva.

Exp Eye Res 2021 04 2;205:108527. Epub 2021 Mar 2.

Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy. Electronic address:

The purpose of this study was to evaluate the expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2 in an immortalized human conjunctival epithelial cell line and in healthy human conjunctiva excised during ocular surgery, using Western blot, confocal microscopy and immunohistochemistry. The Western blot showed that ACE2 and TMPRSS2 proteins were expressed in human immortalized conjunctival cells, and this was confirmed by confocal microscopy images, that demonstrated a marked cellular expression of the viral receptors and their co-localization on the cell membranes. Healthy conjunctival samples from 11 adult patients were excised during retinal detachment surgery. Read More

View Article and Full-Text PDF