76,743 results match your criteria confirmed rna


Gene and Protein Expression of Epithelial to Mesenchymal Transition for Intestinal and Anal Fistula: A Systematic Review.

Ann Coloproctol 2021 Dec 3. Epub 2021 Dec 3.

Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Nilai, Malaysia.

Purpose: Intestinal fibrosis is a common complication of inflammatory bowel diseases. However, the possible involvement of epithelial-mesenchymal transition (EMT) has been scarcely investigated. This systematic review aims to search through research papers that are focusing on messenger RNA (mRNA) and protein expression profile in EMT in fistula or in intestinal fibrosis. Read More

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December 2021

Lnc-hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR-497.

Biofactors 2021 Dec 2. Epub 2021 Dec 2.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.

Noncoding RNAs (ncRNAs) such as microRNAs (miRNAs), long noncoding RNAs (lncRNA), and circular RNAs are closely related to the biological processes related to obesity. As a miRNA that widely present in different cell types, miR497 is proved to be involved in cell development. However, research on the role of miR-497 as a key factor in regulating the development of adipocytes is still in gap. Read More

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December 2021

The Alaska Native/American Indian experience of hepatitis C treatment with sofosbuvir-based direct-acting antivirals.

PLoS One 2021 2;16(12):e0260970. Epub 2021 Dec 2.

Alaska Native Tribal Health Consortium, Liver Disease and Hepatitis Program, Anchorage, Alaska, United States of America.

Background: Direct-acting antiviral (DAA) drugs have been effective in the treatment of chronic hepatitis C virus (HCV) infection. Limited data are available on safety, tolerability, and efficacy in American Indian or Alaska Native people. We aim to evaluate the treatment outcomes of sofosbuvir- based regimens for treatment of HCV in a real life setting in Alaska Native/American Indian (AN/AI) people. Read More

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December 2021

DEAD-box Helicase 27 Promotes Hepatocellular Carcinoma Progression Through ERK Signaling.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211055953

12476The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, China.

DEAD-box helicase 27 (DDX27) belongs to DEAD-Box nucleic acid helicase family. The function of DDX27 in hepatocellular carcinoma (HCC) remain enigmatic. In light of this, we tried to investigate the regulatory role and underlying mechanism of DDX27 in HCC. Read More

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December 2021

Liver Disease: Hepatitis C.

FP Essent 2021 Dec;511:23-28

Department of Family Medicine - Tufts University School of Medicine, 136 Harrison Ave, Boston, MA 02111.

Approximately 4.1 million individuals in the United States have a history of hepatitis C virus (HCV) exposure, including 2.5 million with chronic infection. Read More

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December 2021

Exosomal lnc-AFTR as a novel translation regulator of FAS ameliorates Staphylococcus aureus-induced mastitis.

Biofactors 2021 Dec 2. Epub 2021 Dec 2.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Although the specific expression of long noncoding RNA (lncRNA) in mastitis tissue has been reported, few studies have involved the differential expression of lncRNA in mastitis exosomes (Exo) and its mechanism and function. We screened an lncRNA associated with FAS translational regulation (lnc-AFTR) through exosomal RNA sequencing, and clarified its function and molecular mechanism. Lnc-AFTR is markedly downregulated in Staphylococcus aureus-Exo and S. Read More

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December 2021

Ropivacaine Retards the Viability, Migration, and Invasion of Choriocarcinoma Cells by Regulating the Long Noncoding RNA OGFRP1/MicroRNA-4731-5p/HIF3A Axis.

Mol Biotechnol 2021 Dec 2. Epub 2021 Dec 2.

Department of Anaesthesiology, Fudan University Shanghai Cancer Center, No. 270, Dong'an Road, Shanghai City, 200032, China.

Choriocarcinoma is an aggressive gestational trophoblastic neoplasm. This study attempted to explore the biological functions and underlying mechanisms by which ropivacaine restrains the progression of choriocarcinoma. The expression of long noncoding RNA OGFRP1, microRNA-4731-5p (miR-4731-5p), and HIF3A in choriocarcinoma cells was assessed by qRT-PCR. Read More

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December 2021

Single-cell RNA-seq profiling of individual Biomphalaria glabrata immune cells with a focus on immunologically relevant transcripts.

Immunogenetics 2021 Dec 2. Epub 2021 Dec 2.

School of Public Health, University of Alberta, Edmonton, AB, Canada.

The immune cells of the snail Biomphalaria glabrata are classified into hyalinocyte and granulocyte subtypes. Both subtypes are essential for the proper functioning of the snail immune response, which we understand best within the context of how it responds to challenge with the human parasite Schistosoma mansoni. Granulocytes are adherent phagocytic cells that possess conspicuous granules within the cell cytoplasm. Read More

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December 2021

Live imaging of transcription sites using an elongating RNA polymerase II-specific probe.

J Cell Biol 2022 Feb 2;221(2). Epub 2021 Dec 2.

School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.

In eukaryotic nuclei, most genes are transcribed by RNA polymerase II (RNAP2), whose regulation is a key to understanding the genome and cell function. RNAP2 has a long heptapeptide repeat (Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7), and Ser2 is phosphorylated on an elongation form. To detect RNAP2 Ser2 phosphorylation (RNAP2 Ser2ph) in living cells, we developed a genetically encoded modification-specific intracellular antibody (mintbody) probe. Read More

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February 2022

First Report of Penicillium olsonii Bainier & Sartory Causing Postharvest Fruit Rot of Grape (Vitis vinifera L.) in China.

Plant Dis 2021 Dec 2. Epub 2021 Dec 2.

China West Normal University, 56714, Shida Road, Shunqing District, Nanchong, Sichuan, China., Nanchong, China, 637002;

Grapes (Vitis vinifera L.) are very popular in China as fresh fruit. Due to its storability, some grape varieties can be kept fresh until winter, increasing the popularity of fruit grapes. Read More

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December 2021

Upregulated SLC27A2/FATP2 in differentiated thyroid carcinoma promotes tumor proliferation and migration.

J Clin Lab Anal 2021 Dec 2:e24148. Epub 2021 Dec 2.

Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Differentiated thyroid carcinoma (DTC) accounts for the vast majority of thyroid cancer (TC) cases. The rapidly increasing incidence of TC requires the urgent identification of new diagnostic and therapeutic targets. Solute carrier family 27 member 2 (SLC27A2/FATP2) plays an essential role in lipid biosynthesis and fatty acid transport. Read More

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December 2021

A Case Series on Enteroviral Meningitis in Pakistan.

Cureus 2021 Oct 26;13(10):e19048. Epub 2021 Oct 26.

Neurology, Aga Khan University Hospital, Karachi, PAK.

Enteroviruses (EVs) are the most common cause of viral meningitis with a peak incidence between late summer and fall. The onset of symptoms is characteristically abrupt and typically includes headache, fever, nausea or vomiting, malaise, photophobia, and meningismus. In addition, diarrhea, upper respiratory symptoms, and a rash may also be present. Read More

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October 2021

Super-enhancer-driven lncRNA-DAW promotes liver cancer cell proliferation through activation of Wnt/β-catenin pathway.

Mol Ther Nucleic Acids 2021 Dec 3;26:1351-1363. Epub 2021 Nov 3.

Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou, P.R. China.

Aberrant expression of long non-coding RNAs (lncRNAs) has been reported in multiple cancers. However, the underlying mechanisms mediated by super-enhancers remain elusive. Here we sought to define the role of a novel lncRNA termed lncRNA-DAW in tumorigenesis. Read More

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December 2021

The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1.

Int J Gen Med 2021 24;14:8785-8795. Epub 2021 Nov 24.

Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Objective: In this study, we aimed to investigate the role of RNA N6-methyladenosine demethylase fat mass and obesity-associated protein () in head and neck squamous cell carcinoma (HNSCC).

Methods: Clinical data downloaded from The Cancer Genome Atlas (TCGA) database were used to analyze the relationship between mRNA levels of , and , and the overall survival in cancer and para-cancer datasets. expression in tumor and normal tissues was compared using immunohistochemistry, and its relationship with overall survival was analyzed based on the Kaplan-Meier method. Read More

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November 2021

ALKBH5-mediated mA demethylation of KCNK15-AS1 inhibits pancreatic cancer progression via regulating KCNK15 and PTEN/AKT signaling.

Cell Death Dis 2021 Dec 1;12(12):1121. Epub 2021 Dec 1.

Department of Hepatopancreatobiliary Surgery, Huai'an Hospital Affiliated to Xuzhou Medical University (Second People's Hospital of Huai'an City), Huai'an, Jiangsu, 223002, China.

Long noncoding RNAs (lncRNAs) are regarded as crucial regulators in tumor progression. Potassium two pore domain channel subfamily K member 15 and WISP2 antisense RNA 1 (KCNK15-AS1) has been confirmed to inhibit the migration and invasion of pancreatic cancer (PC) cells. However, its downstream mechanism and effect on other cellular functions in PC remain unknown. Read More

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December 2021

Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study.

BMC Oral Health 2021 12 1;21(1):614. Epub 2021 Dec 1.

Department of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310006, China.

Background: Hypha essential genes (HEGs) of Candida Albicans have been emerging into scholar's attention, little known about their functions in oral lichen planus (OLP) with an uncovered etiology. This research aimed to observe necessary genes in biphasic C. albicans from OLP and study their relevance in pathogenesis, so as to evaluate possible roles of morphologic switching in etiology of OLP. Read More

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December 2021

Circular RNA circFLNA inhibits the development of bladder carcinoma through microRNA miR-216a-3p/BTG2 axis.

Bioengineered 2021 Dec;12(2):11376-11389

Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.

Recent studies have shown that circular RNA circFLNA is abnormally expressed in a variety of malignant tumors, but its role and mechanism in bladder carcinoma (BCa) are still unclear. The present paper aims to contribute to research on the effects and mechanism of circFLNA on the malignant phenotype of BCa. In this study, the expressions of circFLNA, miR-216a-3p and BTG2 in BCa and BCa cells (EJ, T24, 5637, TCC-SUP) were detected by qRT-PCR. Read More

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December 2021

Downregulated circular RNA hsa_circ_0005797 inhibits endometrial cancer by modulating microRNA-298/Catenin delta 1 signaling.

Bioengineered 2021 Dec 1. Epub 2021 Dec 1.

Department of Gynecology, New District Hospital,The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang 471003, China.

Abnormal expression of circular RNA (circRNA) expression has been implicated in endometrial cancer (EC) progression. Thus, investigation of the mechanism of hsa_circ_0005797 during EC etiology may provide new insight into the treatment of EC. In the present study, we found that hsa_circ_0005797 expression was significantly increased in EC biological samples and cell lines, whereas its downregulation inhibited tumor cells proliferation and invasion phenotypes and suppressed tumor formation in nude mice. Read More

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December 2021

Hsa_circ_0030586 promotes epithelial-mesenchymal transition in prostate cancer via PI3K-AKT signaling.

Bioengineered 2021 Dec;12(2):11089-11107

Department of Urology, Zhongshan Hospital Xiamen University, Xiamen, Fujian, China.

Circular RNAs (CircRNAs) gain importance as regulatory molecules in prostate cancer (PCa), but molecular mechanism of most circRNAs in pathogenesis of PCa remains to be studied. This study aimed to explore the role of hsa_circ_0030586 in PCa. Gene Expression Omnibus database (GSE77661) was used to screen out candidate circRNAs. Read More

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December 2021

Long non‑coding RNA MBI‑52 inhibits the development of liver fibrosis by regulating the microRNA‑466g/SMAD4 signaling pathway.

Mol Med Rep 2022 Jan 1;25(1). Epub 2021 Dec 1.

Department of Interventional Radiology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710038, P.R. China.

Liver fibrosis is a wound healing response triggered by liver injury. In severe cases, it may develop into liver cirrhosis, liver cancer and liver failure. Long non‑coding RNAs (lncRNAs) play key roles in the development of liver fibrosis. Read More

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January 2022

Inhibition of lncRNA NEAT1 protects endothelial cells against hypoxia/reoxygenation‑induced NLRP3 inflammasome activation by targeting the miR‑204/BRCC3 axis.

Mol Med Rep 2022 Jan 1;25(1). Epub 2021 Dec 1.

Department of Anesthesiology, Shenzhen Bao'an Maternity and Child Health Hospital, Shenzhen, Guangdong 518100, P.R. China.

Cardiovascular ischemia/reperfusion (I/R) injury is primarily caused by oxygen recovery after prolonged hypoxia. Previous studies found that the long non coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) was involved in cardiovascular pathology, and that NOD‑like receptor protein 3 (NLRP3) inflammasome activation‑dependent pyroptosis played a key role in cardiovascular I/R injury. The present study aimed to explore the molecular mechanism of I/R pathogenesis in order to provide novel insights for potential future therapies. Read More

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January 2022

Gene Expression Risk Scores for COVID-19 Illness Severity.

J Infect Dis 2021 Nov 30. Epub 2021 Nov 30.

Division of Neonatology and Pediatric Molecular and Personalized Medicine Program, Department of Pediatrics.

Background: The correlates of COVID-19 illness severity following infection with SARS-Coronavirus 2 (SARS-CoV-2) are incompletely understood.

Methods: We assessed peripheral blood gene expression in 53 adults with confirmed SARS-CoV-2-infection clinically adjudicated as having mild, moderate or severe disease. Supervised principal components analysis was used to build a weighted gene expression risk score (WGERS) to discriminate between severe and non-severe COVID. Read More

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November 2021

Shoot and root single cell sequencing reveals tissue- and daytime-specific transcriptome profiles.

Plant Physiol 2021 Nov 19. Epub 2021 Nov 19.

Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm, Am MĂĽhlenberg 1, 14476 Potsdam, Germany.

Although several large-scale single-cell RNA sequencing (scRNAseq) studies addressing the root of Arabidopsis (Arabidopsis thaliana) have been published, there is still need for a de novo reference map for both root and especially above-ground cell types. As the plants' transcriptome substantially changes throughout the day, shaped by the circadian clock, we performed scRNAseq on both Arabidopsis root and above-ground tissues at defined times of the day. For the root scRNAseq analysis, we used tissue-specific reporter lines grown on plates and harvested at the end of the day (ED). Read More

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November 2021

Novel RAF Fusions in Pediatric Low-Grade Gliomas Demonstrate MAPK Pathway Activation.

J Neuropathol Exp Neurol 2021 Nov 28. Epub 2021 Nov 28.

From the Department of Pediatrics, University of Colorado School of Medicine, Children's Hospital Colorado, Aurora, Colorado, USA (KTL); Department of Pediatrics, University of Colorado School of Medicine, Morgan Adams Foundation Pediatric Brain Tumor Research Program, Aurora, Colorado, USA (HVC, JD, PC, BS, AD, CAE, AJK, ALG); and Department of Pathology, University of Colorado School of Medicine, Aurora, Colorado, USA (KD, NW, AG).

Brain tumors are the most common solid tumor in children, and low-grade gliomas (LGGs) are the most common childhood brain tumor. Here, we report on 3 patients with LGG harboring previously unreported or rarely reported RAF fusions: FYCO1-RAF1, CTTNBP2-BRAF, and SLC44A1-BRAF. We hypothesized that these tumors would show molecular similarity to the canonical KIAA1549-BRAF fusion that is the most widely seen alteration in pilocytic astrocytoma (PA), the most common pediatric LGG variant, and that this similarity would include mitogen-activated protein kinase (MAPK) pathway activation. Read More

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November 2021

Novel role of prostate cancer risk variant rs7247241 on PPP1R14A isoform transition through allelic TF binding and CpG methylation.

Hum Mol Genet 2021 Nov 29. Epub 2021 Nov 29.

Department of Tumor Biology, H. Lee Moffitt Cancer Center & Research Institute, 12902 Magnolia Drive, Tampa, FL, 33612, United States.

Although previous studies identified numerous single nucleotide polymorphisms (SNPs) and their target genes predisposed to prostate cancer (PrCa) risks, SNP-related splicing associations are rarely reported. In this study, we applied distance-based sQTL analysis (sQTLseekeR) using RNA-seq and SNP genotype data from benign prostate tissue (n = 467), and identified significant associations in 3344 SNP-transcript pairs (P ≤ 0.05) at PrCa risk loci. Read More

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November 2021

Identification of Unique Transcriptomic Signatures and Hub Genes Through RNA Sequencing and Integrated WGCNA and PPI Network Analysis in Nonerosive Reflux Disease.

J Inflamm Res 2021 23;14:6143-6156. Epub 2021 Nov 23.

Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People's Republic of China.

Purpose: Transcriptomic studies on gastroesophageal reflux disease are scarce, and gene expression signatures in nonerosive reflux disease (NERD) remain elusive. The aim of the study was to identify gene expression profiles and potential hub genes in NERD.

Patients And Methods: We performed RNA sequencing on biopsy samples from nine consecutive patients with NERD and six healthy controls. Read More

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November 2021

Silencing TXNIP ameliorates high uric acid-induced insulin resistance via the IRS2/AKT and Nrf2/HO-1 pathways in macrophages.

Free Radic Biol Med 2021 Nov 27. Epub 2021 Nov 27.

Department of Internal Medicine, Xiang'an Hospital of Xiamen University, School of Medicine Xiamen University, Xiamen, China; Department of Diabetes, Endocrinology and Clinical Immunology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan; Xiamen Key Laboratory of Translational Medicine for Nucleic Acid Metabolism and Regulation, Xiamen, Fujian, China. Electronic address:

Insulin resistance (IR) promotes atherosclerosis and increases the risk of diabetes and cardiovascular diseases. Our previous studies have demonstrated that high uric acid (HUA) increased oxidative stress, leading to IR in cardiomyocytes and pancreatic β cells. However, whether HUA can induce IR in monocytes/macrophages, which play critical roles in all stages of atherosclerosis, is unclear. Read More

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November 2021

CircCRIM1 promotes ovarian cancer progression by working as ceRNAs of CRIM1 and targeting miR-383-5p/ZEB2 axis.

Reprod Biol Endocrinol 2021 Nov 30;19(1):176. Epub 2021 Nov 30.

Department of Obstetrics and Gynecology, Department of Gynecologic Oncology Research Office, Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.

Background: Ovarian cancer is the leading cause of death in patients with gynecologic cancer, and circular RNAs (circRNAs) are involved in cancer progression. However, there are limited studies on the roles of circRNAs in ovarian cancer.

Methods: We designed divergent and convergent primers, used sanger sequencing and RNase R digestion to verify the source of circCRIM1. Read More

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November 2021

Screening of Severe Acute Respiratory Syndrome Coronavirus 2 RNA-Dependent RNA Polymerase Inhibitors Using Computational Approach.

J Comput Biol 2021 Nov 29. Epub 2021 Nov 29.

Department of Biotechnology, Indian Institute of Technology, Roorkee, India.

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November 2021

Trichinella spiralis: Knockdown of gamma interferon inducible lysosomal thiol reductase (GILT) results in the reduction of worm burden.

PLoS Negl Trop Dis 2021 Nov 30;15(11):e0009958. Epub 2021 Nov 30.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Trichinella spiralis is mammalian skeletal muscles parasite which may cause trichinellosis in animals and humans. Gamma interferon inducible lysosomal thiol reductase (GILT) is a widespread superfamily which plays key role in processing and presentation of MHC class II restricted antigen by catalyzing disulfide bond reduction. There are no reports about GILT in T. Read More

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November 2021