Microb Biotechnol 2021 05 19;14(3):1107-1119. Epub 2021 Mar 19.
Laboratory of Structural Chemistry and Biology, Institute of Chemistry, Eötvös Loránd University, Pázmány P. stny. 1/A, Budapest, H-1117, Hungary.
We developed a cost sensitive isotope labelling procedure using a fed-batch fermentation method and tested its efficiency producing the N-, C- and N/ C-labelled variants of an amyloidogenic miniprotein (E5: EEEAVRLYIQWLKEGGPSSGRPPPS). E5 is a surface active protein, which forms amyloids in solution. Here, we confirm, using both PM-IRRAS and AFM measurements, that the air-water interface triggers structural rearrangement and promotes the amyloid formation of E5, and thus it is a suitable test protein to work out efficient isotope labelling schemes even for such difficult sequences. Read More