1,027 results match your criteria confirm rearrangement

Challenging Diagnosis in NUT Carcinoma.

Int J Surg Pathol 2021 Jun 9:10668969211019532. Epub 2021 Jun 9.

Institute of Pathology, Kepler University Hospital, Linz, Austria.

Nuclear protein in testis (NUT) carcinoma represents a highly aggressive, poorly differentiated carcinoma that is genetically defined by rearrangement of NUT gene. The histomorphological appearance ranges from entirely undifferentiated carcinoma to carcinoma with prominent squamous differentiation. NUT carcinoma can display neuroendocrine features. Read More

View Article and Full-Text PDF

Osteology of the enigmatic threadsnake species Epictia unicolor and Trilepida guayaquilensis (Serpentes, Leptotyphlopidae) with generic insights.

Anat Rec (Hoboken) 2021 Jun 8. Epub 2021 Jun 8.

Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Since the rearrangement of all leptotyphlopid species previously known as "Leptotyphlops" in Adalsteinsson et al. (2009)'s paper, several taxa have remained untested regarding their generic identity and have been assigned to different genera based on phenotypic data or, in some cases, without any clear justification. Most of the difficulties in assigning some leptotyphlopid taxa are due to their conserved external morphology, summed with a relatively small number of available specimens, which complicate the recognition of a unique combination of characters for their reasonable generic allocation. Read More

View Article and Full-Text PDF

Electrosynthesis of 1,4-bis(diphenylphosphanyl) tetrasulfide via sulfur radical addition as cathode material for rechargeable lithium battery.

Nat Commun 2021 May 28;12(1):3220. Epub 2021 May 28.

College of Chemistry, Zhengzhou University, Zhengzhou, P. R. China.

Organic electrodes are promising as next generation energy storage materials originating from their enormous chemical diversity and electrochemical specificity. Although organic synthesis methods have been extended to a broad range, facile and selective methods are still needed to expose the corners of chemical space. Herein, we report the organopolysulfide, 1,4-bis(diphenylphosphanyl)tetrasulfide, which is synthesized by electrochemical oxidation of diphenyl dithiophosphinic acid featuring the cleavage of a P-S single bond and a sulfur radical addition reaction. Read More

View Article and Full-Text PDF

Use of Fatty Acid Metal Salts for Preventing Maillard Reaction-Driven Browning of Lecithins.

J Oleo Sci 2021 Jun 7;70(6):769-775. Epub 2021 May 7.

Department of Agricultural Chemistry, Faculty of Agriculture and Marine Science, Kochi University.

We previously reported that fluid soybean lecithin (SL) undergoes heat deterioration by the newly reported pseudo-Maillard rearrangement reaction. To inhibit heat deterioration, SLs were treated with metal silicates, such as magnesium silicate and calcium silicate. When soybean fatty acid was added to SL before treatment with calcium silicate, the color index after heating improved significantly as the acid value increased from 10 to 35 mg KOH/g. Read More

View Article and Full-Text PDF

Observation and mechanism of cryo N cleavage by a tantalum cluster.

Phys Chem Chem Phys 2021 May;23(19):11345-11354

Fachbereich Chemie and Forschungszentrum OPTIMAS, Technische Universität Kaiserslautern, 67663 Kaiserslautern, Germany.

We explore the cryogenic kinetics of N2 adsorption to Ta4+ and the infrared signatures of [Ta4(N2)m]+ complexes, m = 1-5. This is accomplished by N2 exposure of isolated ions within a cryogenic ion trap. We find stepwise addition of numerous N2 molecules to the Ta4+ cluster. Read More

View Article and Full-Text PDF

MRCKβ links Dasm1 to actin rearrangements to promote dendrite development.

J Biol Chem 2021 Apr 29:100730. Epub 2021 Apr 29.

NHC Key Laboratory of Glycoconjugate Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China;. Electronic address:

Proper dendrite morphogenesis and synapse formation are essential for neuronal development and function. Dasm1, a member of the immunoglobulin superfamily, is known to promote dendrite outgrowth and excitatory synapse maturation in vitro. However, the in vivo function of Dasm1 in neuronal development and the underlying mechanisms are not well understood. Read More

View Article and Full-Text PDF

Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching.

Neural Regen Res 2021 Dec;16(12):2549-2556

Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province, China.

Cytoskeletal microtubule rearrangement and movement are crucial in the repair of spinal cord injury. Spastin plays an important role in the regulation of microtubule severing. Both spastin and collapsin response mediator proteins can regulate neurite growth and branching; however, whether spastin interacts with collapsin response mediator protein 3 (CRMP3) during this process remains unclear, as is the mechanism by which CRMP3 participates in the repair of spinal cord injury. Read More

View Article and Full-Text PDF
December 2021

Mitochondrial analysis of oribatid mites provides insights into their atypical tRNA annotation, genome rearrangement and evolution.

Parasit Vectors 2021 Apr 23;14(1):221. Epub 2021 Apr 23.

Department of Health Inspection and Quarantine, Wannan Medical College, Wuhu, Anhui Province, 241002, People's Republic of China.

Background: The mitochondrial (mt) genomes of Sarcoptiformes mites typically contain 37 genes. Although the loss of genes is rare in Sarcoptiformes mite mitogenomes, two of the six previously reported oribatid mites (Acariforms: Sarcoptiformes) are reported to have lost parts of their tRNA genes. To confirm whether the tRNA genes were indeed lost and whether the loss is universal, we re-annotated the available oribatid mite sequences and sequenced the mitogenome of Oribatula sakamorii. Read More

View Article and Full-Text PDF

Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors.

Elife 2021 Apr 21;10. Epub 2021 Apr 21.

Physiology, Biophysics and Systems Biology Graduate Program, Weill Cornell Graduate School of Medical Sciences, New York, United States.

The metabotropic glutamate receptors (mGluRs) form a family of neuromodulatory G-protein-coupled receptors that contain both a seven-helix transmembrane domain (TMD) and a large extracellular ligand-binding domain (LBD) which enables stable dimerization. Although numerous studies have revealed variability across subtypes in the initial activation steps at the level of LBD dimers, an understanding of inter-TMD interaction and rearrangement remains limited. Here, we use a combination of single molecule fluorescence, molecular dynamics, functional assays, and conformational sensors to reveal that distinct TMD assembly properties drive differences between mGluR subtypes. Read More

View Article and Full-Text PDF

Molecular diagnosis in non-small-cell lung cancer: expert opinion on and testing.

J Clin Pathol 2021 Apr 19. Epub 2021 Apr 19.

Department of Pathology, Hospital Universitario Virgen del Rocío, Sevilla, Spain.

The effectiveness of targeted therapies with tyrosine kinase inhibitors in non-small-cell lung cancer (NSCLC) depends on the accurate determination of the genomic status of the tumour. For this reason, molecular analyses to detect genetic rearrangements in some genes (ie, , , ) have become standard in patients with advanced disease. Since immunohistochemistry is easier to implement and interpret, it is normally used as the screening procedure, while fluorescence in situ hybridisation (FISH) is used to confirm the rearrangement and decide on ambiguous immunostainings. Read More

View Article and Full-Text PDF

DNA Double-Strand Breaks Affect Chromosomal Rearrangements during Methotrexate-Mediated Gene Amplification in Chinese Hamster Ovary Cells.

Pharmaceutics 2021 Mar 12;13(3). Epub 2021 Mar 12.

Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.

Methotrexate (MTX)-mediated gene amplification has been widely used in Chinese hamster ovary (CHO) cells for the biomanufacturing of therapeutic proteins. Although many studies have reported chromosomal instability and extensive chromosomal rearrangements in MTX-mediated gene-amplified cells, which may be associated with cell line instability issues, the mechanisms of chromosomal rearrangement formation remain poorly understood. We tested the impact of DNA double-strand breaks (DSBs) on chromosomal rearrangements using bleomycin, a DSB-inducing reagent. Read More

View Article and Full-Text PDF

Allosteric Kinase Inhibitors Reshape MEK1 Kinase Activity Conformations in Cells and In Silico.

Biomolecules 2021 Mar 30;11(4). Epub 2021 Mar 30.

Institute of Biochemistry and Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria.

Mutations at different stages of the mitogen-activated protein kinase (MAPK) signaling pathway lead to aberrant activation of the involved protein kinase entities. These oncogenic modifications alter signal propagation which converge on the gatekeeper kinases MEK1/2, transmitting the input signal to ERK1/2. Thus, targeted MEK inhibition causes qualitative alterations of carcinogenic MAPK signals. Read More

View Article and Full-Text PDF

Single-Cell Sequencing Confirms Transcripts and VDJ Rearrangements of Immunoglobulin Genes in Human Podocytes.

Genes (Basel) 2021 Mar 25;12(4). Epub 2021 Mar 25.

Department of Nephrology, Peking University Third Hospital, Beijing 100191, China.

Most glomerular diseases are associated with inflammation caused by deposited pathogenic immunoglobulins (Igs), which are believed to be produced by B cells. However, our previous study indicated that the human podocyte cell line can produce IgG. In this study, we aimed to confirm the transcripts and characterize the repertoires of Igs in primary podocytes at single cell level. Read More

View Article and Full-Text PDF

Recent updates in salivary gland tumors of the lung.

Anja C Roden

Semin Diagn Pathol 2021 Mar 11. Epub 2021 Mar 11.

Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, MN, United States. Electronic address:

Salivary gland tumors are uncommon primary lesions in the lung. Their morphologic, immunophenotypic, and molecular characteristics resemble those of their counterparts in the head and neck or elsewhere. Most common primary pulmonary salivary gland tumors include mucoepidermoid carcinoma, adenoid cystic carcinoma, and epithelial-myoepithelial carcinoma. Read More

View Article and Full-Text PDF

Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins.

Microb Biotechnol 2021 05 19;14(3):1107-1119. Epub 2021 Mar 19.

Laboratory of Structural Chemistry and Biology, Institute of Chemistry, Eötvös Loránd University, Pázmány P. stny. 1/A, Budapest, H-1117, Hungary.

We developed a cost sensitive isotope labelling procedure using a fed-batch fermentation method and tested its efficiency producing the N-, C- and N/ C-labelled variants of an amyloidogenic miniprotein (E5: EEEAVRLYIQWLKEGGPSSGRPPPS). E5 is a surface active protein, which forms amyloids in solution. Here, we confirm, using both PM-IRRAS and AFM measurements, that the air-water interface triggers structural rearrangement and promotes the amyloid formation of E5, and thus it is a suitable test protein to work out efficient isotope labelling schemes even for such difficult sequences. Read More

View Article and Full-Text PDF

Involvement of β-catenin in Androgen-induced Mesenchymal Transition of Breast MDA-MB-453 Cancer Cells.

Endocr Res 2021 Mar 11:1-15. Epub 2021 Mar 11.

Department of Physiology and Biochemistry, School of Medicine, the University of Jordan, Amman, Jordan.

The cellular and molecular dynamics of DHT-induced EMT in MDA-MB-453 cells were investigated.:PCR arrays were used to examine the expression of EMT-regulatory genes. Immunoblotting was used to detect protein levels and confirm protein-protein interaction following immunoprecipitation. Read More

View Article and Full-Text PDF

Novel SLC12A2-ROS1 Fusion in Non-Small Cell Lung Cancer with a Significant Response to Crizotinib: The Importance of Choosing the Appropriate Next-Generation Sequencing Assay.

Oncologist 2021 Jun 26;26(6):e908-e912. Epub 2021 Mar 26.

Department of Medical Oncology, Hospital Universitario La Paz, Madrid, Spain.

Identifying the druggable target is crucial for patients with nonsquamous advanced non-small cell lung cancer (NSCLC). This article adds to the spectrum of ROS1 fusion cases described in NSCLC. We describe a novel SLC12A2-ROS1 rearrangement that has not been previously reported in other cancers: a fusion that has clinical and radiological sensitivity to crizotinib. Read More

View Article and Full-Text PDF

Head to head evaluation of second generation ALK inhibitors brigatinib and alectinib as first-line treatment for ALK+ NSCLC using an systems biology-based approach.

Oncotarget 2021 Feb 16;12(4):316-332. Epub 2021 Feb 16.

Anaxomics Biotech, Barcelona, Spain.

Around 3-7% of patients with non-small cell lung cancer (NSCLC), which represent 85% of diagnosed lung cancers, have a rearrangement in the ALK gene that produces an abnormal activity of the ALK protein cell signaling pathway. The developed ALK tyrosine kinase inhibitors (TKIs), such as crizotinib, ceritinib, alectinib, brigatinib and lorlatinb present good performance treating ALK+ NSCLC, although all patients invariably develop resistance due to ALK secondary mutations or bypass mechanisms. In the present study, we compare the potential differences between brigatinib and alectinib's mechanisms of action as first-line treatment for ALK+ NSCLC in a systems biology-based setting. Read More

View Article and Full-Text PDF
February 2021

Oral and oropharyngeal diffuse large B-cell lymphoma and high-grade B-cell lymphoma: A clinicopathologic and prognostic study of 69 cases.

Oral Surg Oral Med Oral Pathol Oral Radiol 2021 Apr 20;131(4):452-462.e4. Epub 2020 Nov 20.

Private Pathology Service, Natal, Rio Grande do Norte, Brazil.

Objective: The objective of this study was to describe the clinicopathological, molecular, and prognostic features of oral/oropharyngeal diffuse large B-cell lymphoma (DLBCL) and high-grade B-cell lymphoma.

Study Design: All cases were retrieved from 7 Brazilian institutions. Immunohistochemical reactions were performed to confirm the diagnoses and to categorize the tumors. Read More

View Article and Full-Text PDF

Identification of tumors with NRG1 rearrangement, including a novel putative pathogenic UNC5D-NRG1 gene fusion in prostate cancer by data-drilling a de-identified tumor database.

Genes Chromosomes Cancer 2021 Jul 24;60(7):474-481. Epub 2021 Feb 24.

Molecular Genetics Department, Bioptická Laboratoř s.r.o., Pilsen, Czech Republic.

The fusion genes containing neuregulin-1 (NRG1) are newly described potentially actionable oncogenic drivers. Initial clinical trials have shown a positive response to targeted treatment in some cases of NRG1 rearranged lung adenocarcinoma, cholangiocarcinoma, and pancreatic carcinoma. The cost-effective large scale identification of NRG1 rearranged tumors is an open question. Read More

View Article and Full-Text PDF

Diagnostic approach in TFE3-rearranged renal cell carcinoma: a multi-institutional international survey.

J Clin Pathol 2021 May 29;74(5):291-299. Epub 2021 Jan 29.

Pathology, University Hospital Center of Martinique, Fort-de-France, Martinique.

Transcription factor E3-rearranged renal cell carcinoma (TFE3-RCC) has heterogenous morphologic and immunohistochemical (IHC) features.131 pathologists with genitourinary expertise were invited in an online survey containing 23 questions assessing their experience on TFE3-RCC diagnostic work-up.Fifty (38%) participants completed the survey. Read More

View Article and Full-Text PDF

Pressure-Induced Formation and Mechanical Properties of 2D Diamond Boron Nitride.

Adv Sci (Weinh) 2021 Jan 11;8(2):2002541. Epub 2020 Dec 11.

Tandon School of Engineering New York University Brooklyn NY 11201 USA.

Understanding phase transformations in 2D materials can unlock unprecedented developments in nanotechnology, since their unique properties can be dramatically modified by external fields that control the phase change. Here, experiments and simulations are used to investigate the mechanical properties of a 2D diamond boron nitride (BN) phase induced by applying local pressure on atomically thin h-BN on a SiO substrate, at room temperature, and without chemical functionalization. Molecular dynamics (MD) simulations show a metastable local rearrangement of the h-BN atoms into diamond crystal clusters when increasing the indentation pressure. Read More

View Article and Full-Text PDF
January 2021

Light-Switchable and Self-Healable Polymer Electrolytes Based on Dynamic Diarylethene and Metal-Ion Coordination.

J Am Chem Soc 2021 Jan 13;143(3):1562-1569. Epub 2021 Jan 13.

Department of Chemistry and Biochemistry, University of California-Santa Barbara, Santa Barbara, California 93106, United States.

Self-healing polymer electrolytes are reported with light-switchable conductivity based on dynamic -donor ligand-containing diarylethene (DAE) and multivalent Ni metal-ion coordination. Specifically, a polystyrene polymer grafted with poly(ethylene glycol--DAE)acrylate copolymer side chains was effectively cross-linked with nickel(II) bis(trifluoromethanesulfonimide) (Ni(TFSI)) salts to form a dynamic network capable of self-healing with fast exchange kinetics under mild conditions. Furthermore, as a photoswitching compound, the DAE undergoes a reversible structural and electronic rearrangement that changes the binding strength of the DAE-Ni complex under irradiation. Read More

View Article and Full-Text PDF
January 2021

Distinctive epigenomic alterations in NF1-deficient cutaneous and plexiform neurofibromas drive differential MKK/p38 signaling.

Epigenetics Chromatin 2021 Jan 13;14(1). Epub 2021 Jan 13.

Center for Cancer and Cell Biology, Van Andel Research Institute, 333 Bostwick Ave. NE, Grand Rapids, MI, 49503, USA.

Benign peripheral nerve sheath tumors are the clinical hallmark of Neurofibromatosis Type 1. They account for substantial morbidity and mortality in NF1. Cutaneous (CNF) and plexiform neurofibromas (PNF) share nearly identical histology, but maintain different growth rates and risk of malignant conversion. Read More

View Article and Full-Text PDF
January 2021

NRG1 fusions in breast cancer.

Breast Cancer Res 2021 Jan 7;23(1). Epub 2021 Jan 7.

Hutchison-MRC Research Centre, University of Cambridge, Cambridge, CB2 0XZ, UK.

Background: NRG1 gene fusions may be clinically actionable, since cancers carrying the fusion transcripts can be sensitive to tyrosine kinase inhibitors. The NRG1 gene encodes ligands for the HER2(ERBB2)-ERBB3 heterodimeric receptor tyrosine kinase, and the gene fusions are thought to lead to autocrine stimulation of the receptor. The NRG1 fusion expressed in the breast cancer cell line MDA-MB-175 serves as a model example of such fusions, showing the proposed autocrine loop and exceptional drug sensitivity. Read More

View Article and Full-Text PDF
January 2021

Evaluation of chromosomal abnormalities from preimplantation genetic testing to the reproductive outcomes: a comparison between three different structural rearrangements based on next-generation sequencing.

J Assist Reprod Genet 2021 Mar 6;38(3):709-718. Epub 2021 Jan 6.

IVF Center, Department of Obstetrics and Gynecology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, Guangdong, China.

Purpose: The aim of this study was to determine factors affecting the chromosome imbalance in blastocysts and reproductive outcomes by a comparison between the reciprocal translocation (REC), inversion (INV), and Robertsonian translocation (ROB) carriers.

Methods: Couples with one partner carrying translocation or inversion underwent preimplantation genetic testing for chromosomal structural rearrangement (PGT-SR) cycles, including 215 PGT-SR cycles performed in subsequent 164 frozen-thawed embryo transfer cycles and 61 prenatal diagnoses of fetuses and 59 normal live birth babies. A total of 899 samples were processed by whole-genome amplification followed by next-generation sequencing (NGS). Read More

View Article and Full-Text PDF

Strong ALK and PD-L1 positive IHC expression related ALK amplification in an advanced lung sarcomatoid carcinoma: A therapeutic trap?

Lung Cancer 2021 02 21;152:94-97. Epub 2020 Dec 21.

Department of Pneumology and Thoracic Oncology, Tenon Hospital, Assistance Publique-Hôpitaux de Paris and GRC 4, Theranoscan, Sorbonne Université, 75970, Paris, France.

Objectives: Immunohistochemistry (IHC) is considered as a screening method for ALK rearrangement thanks to its excellent sensitivity. Strong marking on immunohistochemistry give the go-ahead to start ALK tyrosine kinase inhibitors (ALK TKI). Lack of therapeutic response may then lead to the suspicion of molecular alterations other than ALK rearrangements. Read More

View Article and Full-Text PDF
February 2021

Targeting a conserved pocket (n-octyl-β-D-glucoside) on the dengue virus envelope protein by small bioactive molecule inhibitors.

J Biomol Struct Dyn 2020 Dec 21:1-13. Epub 2020 Dec 21.

Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Tamilnadu, India.

Dengue virus enters the cell by receptor-mediated endocytosis followed by a viral envelope (DENVE) protein-mediated membrane fusion. A small detergent molecule n-octyl-β-D-glucoside (βOG) occupies the hydrophobic pocket which is located in the hinge region plays a major role in the rearrangement. It has been reported that mutations occurred in this binding pocket lead to the alterations of pH threshold for fusion. Read More

View Article and Full-Text PDF
December 2020

Evaluation of NTRK immunohistochemistry as a screening method for NTRK gene fusion detection in non-small cell lung cancer.

Lung Cancer 2021 01 27;151:53-59. Epub 2020 Nov 27.

Dept. of Immunology, Genetics and Pathology, Uppsala, Sweden. Electronic address:

Purpose: The small molecule inhibitors larotrectinib and entrectinib have recently been approved as cancer agnostic drugs in patients with tumours harbouring a rearrangement of the neurotrophic tropomyosin receptor kinase (NTRK). These oncogenic fusions are estimated to occur in 0.1-3 % of non-small cell lung cancers (NSCLC). Read More

View Article and Full-Text PDF
January 2021

Néel Vector Induced Manipulation of Valence States in the Collinear Antiferromagnet MnAu.

ACS Nano 2020 Nov 25. Epub 2020 Nov 25.

Institut für Physik, Johannes Gutenberg-Universität, Staudingerweg 7, D-55099 Mainz, Germany.

The coupling of real and momentum space is utilized to tailor electronic properties of the collinear metallic antiferromagnet MnAu by aligning the real space Néel vector indicating the direction of the staggered magnetization. Pulsed magnetic fields of 60 T were used to orient the sublattice magnetizations of capped epitaxial MnAu(001) thin films perpendicular to the applied field direction by a spin-flop transition. The electronic structure and its corresponding changes were investigated by angular-resolved photoemission spectroscopy with photon energies in the vacuum-ultraviolet, soft, and hard X-ray range. Read More

View Article and Full-Text PDF
November 2020