11,057 results match your criteria conducted porcine


Effects of different dietary protein levels on intestinal aquaporins in weaned piglets.

J Anim Physiol Anim Nutr (Berl) 2022 May 19. Epub 2022 May 19.

College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, P.R. China.

This study was conducted to investigate the relationship between changes in intestinal aquaporins (AQPs) in piglets fed diets with different protein levels and nutritional diarrhoea in piglets. Briefly, 96 weaned piglets were randomly divided into four groups fed diets with crude protein (CP) levels of 18%, 20%, 22% and 24%. The small intestines and colons of the weaned piglets were collected, and several experiments were conducted. Read More

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Frequency and distribution of H1N1 influenza A viruses with oseltamivir-resistant mutations worldwide before and after the 2009 pandemic.

J Med Virol 2022 May 18. Epub 2022 May 18.

College of Global Change and Earth System Science, Beijing Normal University, Beijing, 100875, China.

H1N1 influenza has brought serious threats to people's health and a high socio-economic burden to society. Oseltamivir, a kind of neuraminidase (NA) inhibitor, is the second-generation specific drug that is broadly used currently. However, H1N1 influenza viruses have exhibited oseltamivir resistance in the past decades, which might be a hidden danger. Read More

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Swine coronaviruses (SCoVs) and their emerging threats to swine population, inter-species transmission, exploring the susceptibility of pigs for SARS-CoV-2 and zoonotic concerns.

Vet Q 2022 May 18:1-39. Epub 2022 May 18.

Center of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-10700, Thailand.

Swine coronaviruses (SCoVs) are one of the most devastating pathogens affecting the livelihoods of farmers and swine industry across the world. These include transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine respiratory coronavirus (PRCV), porcine hemagglutinating encephalomyelitis virus (PHEV), swine acute diarrhea syndrome coronavirus (SADS-CoV), and porcine delta coronavirus (PDCoV). Coronaviruses infect a wide variety of animal species and humans because these are having single stranded-RNA that accounts for high mutation rates and thus could break the species barrier. Read More

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Dynamic Distribution of Gut Microbiota in Pigs at Different Growth Stages: Composition and Contribution.

Microbiol Spectr 2022 May 18:e0068821. Epub 2022 May 18.

Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of China, Key Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry of Agriculture of China, Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, and Animal Nutrition Institute, Sichuan Agricultural Universitygrid.80510.3c, Chengdu, People's Republic of China.

Fully understanding the dynamic distribution of the gut microbiota in pigs is essential, as gut microorganisms play a fundamental role in physiological processes, immunity, and the metabolism of nutrients by the host. Here, we first summarize the characteristics and the dynamic shifts in the gut microbial community of pigs at different ages based on the results of 63 peer-review publications. Then a meta-analysis based on the sequences from 16 studies with accession numbers in the GenBank database is conducted to verify the characteristics of the gut microbiota in healthy pigs. Read More

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A diverse English keyword search reveals the value of scriptaid treatment for porcine embryo development following somatic cell nuclear transfer.

Reprod Fertil Dev 2022 May 18. Epub 2022 May 18.

Context: Incomplete epigenetic reprogramming of histone deacetylation (HDAC) is one of the main reasons for the low efficiency of somatic cell nuclear transfer (SCNT). Scriptaid is a synthetic HDAC inhibitor (HDACi) that may improve the efficiency of porcine SCNT.

Aims: This study aimed to determine whether scriptaid increases the number of blastocyst cells or the cleavage rate. Read More

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TPX2 deficiency leads to spindle abnormity and meiotic impairment in porcine oocytes.

Theriogenology 2022 May 8;187:164-172. Epub 2022 May 8.

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

The targeting protein for Xklp2 (TPX2) is a spindle assembly factor, that can stimulate microtubule formation and promote spindle completion during mitosis. However, the role of TPX2 in mammalian oocyte meiotic maturation is still not fully understood. This study was conducted to address the dynamic distribution and potential roles of TPX2 in microtubule nucleation during meiotic maturation in porcine oocytes by microinjecting specific siRNAs. Read More

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Necropsy Procedures and Evaluation of Macroscopic Lesions of Pigs Infected with African Swine Fever Virus.

Methods Mol Biol 2022 ;2503:15-49

Pathology and Animal Sciences Department, Animal and Plant Health Agency (APHA-Weybridge), New Haw, Addlestone, UK.

Pathology complements and provides a fundamental link to other disciplines for disease investigations supporting molecular biology, genetics, immunology, or virology as core basis of scientific research. Necropsies are an essential tool in veterinary pathology for disease investigation and should be conducted in a routine, systematic, and standard approach. An orderly necropsy procedure will allow the prosector (veterinary clinicians or veterinary pathologists) to determine macroscopically normal or altered structures and allow, through experience, to acquire dexterity, speed, and confidence in the technique. Read More

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Enhanced Blood Clotting After Rewarming From Experimental Hypothermia in an Intact Porcine Model.

Front Physiol 2022 29;13:901908. Epub 2022 Apr 29.

Department of Clinical Medicine, Anaesthesia and Critical Care Research Group, UiT The Arctic University of Norway, Tromsø, Norway.

Due to functional alterations of blood platelets and coagulation enzymes at low temperatures, excessive bleeding is a well-recognized complication in victims of accidental hypothermia and may present a great clinical challenge. Still, it remains largely unknown if hemostatic function normalizes upon rewarming. The aim of this study was to investigate effects of hypothermia and rewarming on blood coagulation in an intact porcine model. Read More

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New Porcine Model of Arteriovenous Fistula Documents Increased Coronary Blood Flow at the Cost of Brain Perfusion.

Front Physiol 2022 27;13:881658. Epub 2022 Apr 27.

Institute of Physiology, 1st Faculty of Medicine, Charles University, Prague, Czechia.

Arteriovenous fistulas (AVF) represent a low resistant circuit. It is known that their opening leads to decreased systemic vascular resistance, increased cardiac output and other hemodynamic changes. Possible competition of AVF and perfusion of other organs has been observed before, however the specific impact of AVF has not been elucidated yet. Read More

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CRISPR/Cas9-Mediated Knockout of the and Genes in BHK-21 Cell Promoted Seneca Virus A Replication and Enhanced Autophagy.

Front Cell Infect Microbiol 2022 27;12:865744. Epub 2022 Apr 27.

Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Science, Shandong Normal University, Jinan, China.

RNA interference (RNAi) is a major form of antiviral defense in host cells, and Ago2 and Dicer are the major proteins of RNAi. The Senecavirus A (SVA) is a reemerging virus, resulting in vesicular lesions in sows and a sharp decline in neonatal piglet production. In this study, CRISPR/Cas9 technology was used to knock out Ago2 and Dicer genes in BHK-21 cell lines used for SVA vaccine production. Read More

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The Role of the Wild Boar Spreading African Swine Fever Virus in Asia: Another Underestimated Problem.

Front Vet Sci 2022 27;9:844209. Epub 2022 Apr 27.

VISAVET Health Surveillance Center, Complutense University of Madrid, Madrid, Spain.

African swine fever (ASF) is a highly lethal infectious disease in naive populations of domestic pigs and wild boar. In Asia, from the first outbreak in August 2018 until the end of November 2021, ASF has been reported in 16 Asian countries. The ASF virus (ASFV) circulation in domestic pigs is considered the main problem in Asia. Read More

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Production of Triple-Gene (GGTA1, B2M and CIITA)-Modified Donor Pigs for Xenotransplantation.

Front Vet Sci 2022 28;9:848833. Epub 2022 Apr 28.

Yunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming, China.

Activation of human immune T-cells by swine leukocyte antigens class I (SLA-I) and class II (SLA-II) leads to xenograft destruction. Here, we generated the GGTA1, B2M, and CIITA (GBC) triple-gene-modified miniature pigs, analyzed the transcriptome of GBC-modified peripheral blood mononuclear cells (PBMCs) in the pig's spleen, and investigated their effectiveness in anti-immunological rejection. A total of six cloned piglets were successfully generated using somatic cell nuclear transfer, one of them carrying the heterozygous mutations in triple genes and the other five piglets carrying the homozygous mutations in GGTA1 and CIITA genes, but have the heterozygous mutation in the B2M gene. Read More

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Identification of Homozygous Regions With Adverse Effects on the Five Economic Traits of Duroc Pigs.

Front Vet Sci 2022 28;9:855933. Epub 2022 Apr 28.

College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.

Runs of homozygosity (ROH) are widely used to estimate genomic inbreeding, which is linked to inbreeding depression on phenotypes. However, the adverse effects of specific homozygous regions on phenotypic characteristics are rarely studied in livestock. In this study, the 50 K SNP data of 3,770 S21 Duroc (American origin) and 2,096 S22 Duroc (Canadian origin) pigs were used to investigate the harmful ROH regions on five economic traits. Read More

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Case Report: Severe Anemia Associated With an Abomasal Fibrosarcoma in a Goat.

Front Vet Sci 2022 28;9:869017. Epub 2022 Apr 28.

Clinic for Swine, Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.

A 10-year-old female goat was presented to the clinic with lethargy, emaciation, and pale mucous membranes. Laboratory diagnosis revealed severe anemia with regenerative character as well as melena. Blood transfusions were administered, but the animal's condition continued to deteriorate, so it was euthanized. Read More

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A Novel Immunochromatographic Strip Based on Latex Microspheres for the Rapid Detection of North American-Type Porcine Reproductive and Respiratory Syndrome Virus.

Front Microbiol 2022 29;13:882112. Epub 2022 Apr 29.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.

A latex microsphere-based immunochromatographic strip (ICS) test was successfully developed for the rapid and sensitive detection of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV N protein-specific monoclonal antibody (mAb) 1H4 labeled with latex microspheres was dispensed on a conjugate pad for use as the detector. The same mAb and goat anti-mouse antibody were blotted on a nitrocellulose membrane to generate test and control lines, respectively. Read More

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Gallnut Tannic Acid Exerts Anti-stress Effects on Stress-Induced Inflammatory Response, Dysbiotic Gut Microbiota, and Alterations of Serum Metabolic Profile in Beagle Dogs.

Front Nutr 2022 27;9:847966. Epub 2022 Apr 27.

Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

Stress exposure is a potential threat to humans who live or work in extreme environments, often leading to oxidative stress, inflammatory response, intestinal dysbiosis, and metabolic disorders. Gallnut tannic acid (TA), a naturally occurring polyphenolic compound, has become a compelling source due to its favorable anti-diarrheal, anti-oxidative, anti-inflammatory, and anti-microbial activities. Thus, this study aimed to evaluate the anti-stress effects of gallnut TA on the stress-induced inflammatory response, dysbiotic gut microbiota, and alterations of serum metabolic profile using beagle models. Read More

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Effects of Niacin on Resistance to Enterotoxigenic Infection in Weaned Piglets.

Front Nutr 2022 29;9:865311. Epub 2022 Apr 29.

State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

Nicotinic acid (NA) has been used to treat different inflammatory disease with positive influence, the mechanisms by which NA exerts its anti-inflammatory effects remain largely undefined. Here we proposed a new hypothesis that NA manipulated endogenous antimicrobial peptides (AMPs) which contributed to the elimination of enterotoxigenic (ETEC) K88, and thus affects the alleviation of inflammation. Therefore, an experiment in weaned piglets treated with 40 mg NA for 3 days before ETEC K88 challenge was designed to investigate the effects of NA on resistance to enterotoxigenic infection in weaned piglets. Read More

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Curcumin and Intestinal Oxidative Stress of Pigs With Intrauterine Growth Retardation: A Review.

Front Nutr 2022 28;9:847673. Epub 2022 Apr 28.

CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

Intrauterine growth restriction (IUGR) refers to the slow growth and development of a mammalian embryo/fetus or fetal organs during pregnancy, which is popular in swine production and causes considerable economic losses. Nutritional strategies have been reported to improve the health status and growth performance of IUGR piglets, among which dietary curcumin supplementation is an efficient alternative. Curcumin is a natural lipophilic polyphenol derived from the rhizome of with many biological activities. Read More

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Effect of Baseline Impedance in Radiofrequency Delivery on Lesion Characteristics and the Relationship Between Impedance and Steam Pops.

Front Cardiovasc Med 2022 27;9:872961. Epub 2022 Apr 27.

Department of Cardiology, First Hospital of Shanxi Medical University, Taiyuan, China.

Objective: To explore the effects of baseline impedance (R) and power (P) on radiofrequency ablation (RFA) lesion characteristics and their correlation with steam pops using ThermoCool SmartTouch-SF (STSF) catheters in the porcine heart.

Method: A porcine left ventricle was submerged in 37°C saline , and the experiment was performed with various ( = 30, 40, 50, and 60 W) and multiple loads ( = 80-100, 100-140, 140-180, and 180-220 Ω) to reach the target ablation index (AI; AI = 350, 450, and 500) or reach the target ablation time using a fixed contact force (CF; CF = 10-15 g) and the same saline irrigation (30 W/8 ml/min or 40-60 W/15 ml/min), repeated five times under each condition.

Results: The surface diameter, maximum diameter, depth, and volume of the lesions were strongly correlated with the AI ( = 40 W, = 100-140 Ω, CF = 10-15 g) ( = 0. Read More

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Unveiling the role of dissolved organic matter on phosphorus sorption and availability in a 5-year manure amended paddy soil.

Sci Total Environ 2022 May 12:155892. Epub 2022 May 12.

MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address:

Dissolved organic matter (DOM) is an active component of organic manure that is widely used in agroecosystems to increase nutrient availability and consequently enhance crop yields. However, the ways in which soil DOM characteristics are influenced by organic manure and how it contributes to crop yield and soil P availability remains unclear. Here, we conducted a 5-year field experiment and demonstrated that partial replacement of chemical P fertilizer with swine manure could maintain high rice yield and soil available P levels and increase P fertilizer use efficiency (PUE) in comparison to chemical fertilization, even when the total P input was reduced. Read More

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A DNA Barcode-Based Aptasensor Enables Rapid Testing of Porcine Epidemic Diarrhea Viruses in Swine Saliva Using Electrochemical Readout.

Angew Chem Int Ed Engl 2022 May 13. Epub 2022 May 13.

McMaster University, Biochemistry and Biomedical Sciences, CANADA.

Pen-side testing of farm animals for infectious diseases is critical for preventing transmission in herds and providing timely intervention. However, most existing pathogen tests have to be conducted in centralized labs with sample-to-result times of 2-4 days. Herein we introduce a test that uses a dual-electrode electrochemical chip (DEE-Chip) and a barcode-releasing electroactive aptamer for rapid on-farm detection of porcine epidemic diarrhea viruses (PEDv). Read More

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African Swine Fever (ASF) Trend Analysis in Wild Boar in Poland (2014-2020).

Animals (Basel) 2022 May 3;12(9). Epub 2022 May 3.

Department of Swine Diseases, National Veterinary Research Institute, Partyzantów Avenue 57, 24-100 Puławy, Poland.

African swine fever (ASF) is a lethal hemorrhagic disease of , i.e., domestic pigs and wild boars. Read More

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Potential of Oral Nanoparticles Containing Cytokines as Intestinal Mucosal Immunostimulants in Pigs: A Pilot Study.

Animals (Basel) 2022 Apr 21;12(9). Epub 2022 Apr 21.

Department of Ruminant Production, Institute of Agriculture and Food Research (IRTA), 08140 Caldes de Montbui, Spain.

Antimicrobial resistance is a global threat that is worryingly rising in the livestock sector. Among the proposed strategies, immunostimulant development appears an interesting approach to increase animal resilience at critical production points. The use of nanoparticles based on cytokine aggregates, called inclusion bodies (IBs), has been demonstrated as a new source of immunostimulants in aquaculture. Read More

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Dynamic of Hepatitis E Virus (HEV) Shedding in Pigs.

Animals (Basel) 2022 Apr 20;12(9). Epub 2022 Apr 20.

Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy.

Genotype 3 of hepatitis E virus (HEV-3) is the most common in Europe in both humans and pigs. HEV-3 strains are zoonotic, and foodborne cases associated with consumption of raw and undercooked pork products, mainly liver sausages, have been described. HEV-3 circulates largely in European pig farms, maybe due to its long persistence in the environment. Read More

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Molecular characterization of porcine reproductive and respiratory syndrome virus (PRRSv) identified from slaughtered pigs in northern Uganda.

BMC Vet Res 2022 May 13;18(1):176. Epub 2022 May 13.

College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.

Background: A cross sectional study was conducted to detect and characterize species of porcine reproductive and respiratory syndrome virus (PRRSv) identified from slaughtered pigs in Lira district, northern Uganda. The study was conducted from March to September 2019 in three selected slaughter slabs. Pigs brought for slaughter were randomly sampled. Read More

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Insights into the fates of plasmids and antimicrobial resistance genes during swine manure treatment and related factors based on plasmidome and metagenome analyses.

Environ Sci Pollut Res Int 2022 May 13. Epub 2022 May 13.

Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, 29 Wangjiang Road, Chengdu, 610010, NOSichuan, China.

Swine manure treatment plants are important reservoirs of plasmid-harboring antibiotic resistance genes (ARGs) and physicochemical contaminants, but the changes in the abundances of plasmids and ARGs, and their interactions with the physicochemical properties of manure, are still unclear. Thus, in the present study, plasmidome and metagenome analyses were conducted for samples collected at different stages in the swine manure treatment process. The results indicated that anaerobic digestion and aerobic digestion were the most efficient stages for reducing the abundances of ARGs in swine manure. Read More

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Editorial: African Swine Fever in Smallholder and Traditional Pig Farming Systems: Research, Challenges and Solutions.

Front Vet Sci 2022 26;9:878928. Epub 2022 Apr 26.

Department of Disease Control and Epidemiology, National Veterinary Institute, Uppsala, Sweden.

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Phylogeny, Evolution, and Transmission Dynamics of Canine and Feline Coronaviruses: A Retro-Prospective Study.

Front Microbiol 2022 26;13:850516. Epub 2022 Apr 26.

Swine Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Canine coronavirus (CCoV) and feline coronavirus (FCoV) are endemic in companion animals. Due to their high mutation rates and tendencies of genome recombination, they pose potential threats to public health. The molecular characteristics and genetic variation of both CCoV and FCoV have been thoroughly studied, but their origin and evolutionary dynamics still require further assessment. Read More

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Vaccination With a Gamma Irradiation-Inactivated African Swine Fever Virus Is Safe But Does Not Protect Against a Challenge.

Front Immunol 2022 26;13:832264. Epub 2022 Apr 26.

Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany.

African swine fever (ASF) is among the most devastating viral diseases of pigs and wild boar worldwide. In recent years, the disease has spread alarmingly. Despite intensive research activities, a commercialized vaccine is still not available, and efficacious live attenuated vaccine candidates raise safety concerns. Read More

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cGAS Restricts PRRSV Replication by Sensing the mtDNA to Increase the cGAMP Activity.

Front Immunol 2022 26;13:887054. Epub 2022 Apr 26.

College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.

Porcine reproductive and respiratory syndrome virus (PRRSV) is an RNA virus that causes great economic losses globally to the swine industry. Innate immune RNA receptors mainly sense it during infection. As a DNA sensor, cyclic GMP-AMP synthase (cGAS) plays an important role in sensing cytosolic DNA and activating innate immunity to induce IFN-I and establish an antiviral cellular state. Read More

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