286,117 results match your criteria condition functional

Identifying molecular insight of synergistic complexities for SARS-CoV-2 infection with pre-existing type 2 diabetes.

Comput Biol Med 2021 Jul 23;136:104668. Epub 2021 Jul 23.

Healthy Ageing Theme, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia; WHO Collaborating Centre on eHealth, UNSW Digital Health, School of Public Health and Community Medicine, Faculty of Medicine, UNSW Sydney, NSW, 2052, Australia. Electronic address:

The ongoing COVID-19 outbreak, caused by SARS-CoV-2, has posed a massive threat to global public health, especially to people with underlying health conditions. Type 2 diabetes (T2D) is lethal comorbidity of COVID-19. However, its pathogenetic link remains unclear. Read More

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Hyper- and hypo-connectivity in sensorimotor network of drug-naïve patients with cervical dystonia.

Parkinsonism Relat Disord 2021 Jul 22;90:15-20. Epub 2021 Jul 22.

Center for Movement Disorders, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; China National Clinical Research Center for Neurological Diseases, Beijing, China; Parkinson's Disease Center, Beijing Institute for Brain Disorders, Beijing, China. Electronic address:

Background: Cervical dystonia (CD) is the most common form of focal dystonia with involuntary movements and postures of the head. The pathogenesis and neural mechanisms underlying CD have not been fully elucidated.

Methods: Twenty-seven newly drug-naïve patients with CD and 21 healthy controls (HCs) were recruited with clinical assessment and resting-state functional magnetic resonance imaging (rs-fMRI) scanning. Read More

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Evaluation of S-RBD and high specificity ACE-2-binding antibodies on SARS-CoV-2 patients after six months from infection.

Int Immunopharmacol 2021 Jul 27;99:108013. Epub 2021 Jul 27.

Department of Laboratory Medicine, Tor Vergata University Hospital, Rome, Italy; Department of Experimental Medicine, University of Tor Vergata, Rome, Italy.

The antibody response to SARS-CoV-2 has not yet fully defined, but the availability of sensitive and specific serological assays is crucial to observe the presence of specific antibodies against the human receptor binding domain (S-RBD) and high specificity ACE-2-binding antibodies or neutralizing antibodies (NT) in response to vaccines. Indeed, these peculiar antibodies should prevent viral interaction between RBD and Angiotensin-Converting Enzyme 2 (ACE2) receptor, located on surface of host cells. In this study, 72 samples from 37 hospitalized COVID-19 patients and 35 not-hospitalized patients were analyzed longitudinally. Read More

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Iron status, fibroblast growth factor 23 and cardiovascular and kidney outcomes in chronic kidney disease.

Kidney Int 2021 Jul 30. Epub 2021 Jul 30.

Division of Nephrology, Department of Medicine, and Duke Clinical Research Institute, Duke University School of Medicine, Durham, NC USA.

Disordered iron and mineral homeostasis are interrelated complications of chronic kidney disease that may influence cardiovascular and kidney outcomes. In a prospective analysis of 3747 participants in the Chronic Renal Insufficiency Cohort Study, we investigated risks of mortality, heart failure, end-stage kidney disease (ESKD), and atherosclerotic cardiovascular disease according to iron status, and tested for mediation by C-terminal fibroblast growth factor 23 (FGF23), hemoglobin and parathyroid hormone. Study participants were agnostically categorized based on quartiles of transferrin saturation and ferritin as: "Iron Replete" (27. Read More

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Abnormality in the NK cell population is prolonged in severe COVID-19 patients.

J Allergy Clin Immunol 2021 Jul 30. Epub 2021 Jul 30.

Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea. Electronic address:

Background: Our understanding of adaptive immune responses in COVID-19 patients is rapidly evolving, but information on the innate immune responses by natural killer (NK) cells is still insufficient.

Objective: We aimed to examine the phenotypic and functional status of NK cells and their changes during the course of mild and severe COVID-19.

Methods: We performed RNA sequencing (RNA-seq) and flow cytometric analysis of NK cells from patients with mild and severe COVID-19 at multiple time points in the course of the disease using cryopreserved peripheral blood mononuclear cells (PBMCs). Read More

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Efficiency and microbial diversity of aeration solid-phase denitrification process bioaugmented with HN-AD bacteria for the treatment of low C/N wastewater.

Environ Res 2021 Jul 30:111786. Epub 2021 Jul 30.

School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China. Electronic address:

To evaluate the simultaneous nitrification and denitrification (SND) performance of the aeration solid-phase denitrification (SPD) process and improve the operating efficiency, aeration SPD process using polybutanediol succinate as carbon source was optimized and the process was bioaugmented with heterotrophic nitrification-aerobic denitrification bacteria for the treatment of real wastewater. The results showed that after bioaugmentation, the total nitrogen removal efficiency of the aeration SPD process increased by 50.46 % under condition of dissolved oxygen (DO) 3 mg/L. Read More

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Epidemiology of anal human papillomavirus infection and high-grade squamous intraepithelial lesions in 29 900 men according to HIV status, sexuality, and age: a collaborative pooled analysis of 64 studies.

Lancet HIV 2021 Jul 30. Epub 2021 Jul 30.

Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Background: Robust age-specific estimates of anal human papillomavirus (HPV) and high-grade squamous intraepithelial lesions (HSIL) in men can inform anal cancer prevention efforts. We aimed to evaluate the age-specific prevalence of anal HPV, HSIL, and their combination, in men, stratified by HIV status and sexuality.

Methods: We did a systematic review for studies on anal HPV infection in men and a pooled analysis of individual-level data from eligible studies across four groups: HIV-positive men who have sex with men (MSM), HIV-negative MSM, HIV-positive men who have sex with women (MSW), and HIV-negative MSW. Read More

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The ALPK1 pathway drives the inflammatory response to Campylobacter jejuni in human intestinal epithelial cells.

PLoS Pathog 2021 Aug 2;17(8):e1009787. Epub 2021 Aug 2.

Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.

The Gram-negative bacterium Campylobacter jejuni is a major cause of foodborne disease in humans. After infection, C. jejuni rapidly colonizes the mucus layer of the small and large intestine and induces a potent pro-inflammatory response characterized by the production of a large repertoire of cytokines, chemokines, and innate effector molecules, resulting in (bloody) diarrhea. Read More

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Topology-preserving smoothing of retinotopic maps.

PLoS Comput Biol 2021 Aug 2;17(8):e1009216. Epub 2021 Aug 2.

School of Computing, Informatics, and Decision Systems Engineering, Arizona State University, Tempe, Arizona, United States of America.

Retinotopic mapping, i.e., the mapping between visual inputs on the retina and neuronal activations in cortical visual areas, is one of the central topics in visual neuroscience. Read More

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Categorical interoception: the role of disease context and individual differences in habitual symptom reporting.

Psychol Health 2021 Aug 2:1-19. Epub 2021 Aug 2.

Research Group on Health Psychology, University of Leuven, Leuven, Belgium.

Objective: Symptom reports correspond less to physiological dysfunction in persons with high levels of symptoms in daily life and in patients with functional somatic symptoms, suggesting poor symptom perception. In this study, we investigated whether interoception was impacted by the meaning of the context and by habitual symptom reporting.

Methods: Eight inspiratory resistances that were equidistant in intensity were administered to healthy women (N = 124) varying in habitual symptom reporting. Read More

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Developmental lineage of human pluripotent stem cell-derived cardiac fibroblasts affects their functional phenotype.

FASEB J 2021 Sep;35(9):e21799

Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA.

Cardiac fibroblasts (CFBs) support heart function by secreting extracellular matrix (ECM) and paracrine factors, respond to stress associated with injury and disease, and therefore are an increasingly important therapeutic target. We describe how developmental lineage of human pluripotent stem cell-derived CFBs, epicardial (EpiC-FB), and second heart field (SHF-FB) impacts transcriptional and functional properties. Both EpiC-FBs and SHF-FBs exhibited CFB transcriptional programs and improved calcium handling in human pluripotent stem cell-derived cardiac tissues. Read More

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September 2021

MiR-96-5p is an oncogene in lung adenocarcinoma and facilitates tumor progression through ARHGAP6 downregulation.

J Appl Genet 2021 Aug 2. Epub 2021 Aug 2.

Department of Cardiothoracic Surgery, Zhejiang Provincial People's Hospital (People's Hospital of Hangzhou Medical College), NO. 158 Shangtang Road, Xiacheng District, Hangzhou, 310014, Zhejiang, China.

Accumulating investigations illustrated that miRNA acts as a key regulator in tumor progression, whereas regulatory role of miR-96-5p in lung adenocarcinoma (LUAD) is warranted. Thus, we sought to probe mechanism of miR-96-5p in this disease. Through bioinformatics analysis, miR-96-5p level in normal tissue and LUAD tissue in TCGA database were obtained. Read More

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Linking genome variants to disease: Scalable approaches to test the functional impact of human mutations.

Hum Mol Genet 2021 Aug 2. Epub 2021 Aug 2.

The Francis Crick Institute, The Genome Function Laboratory, 1 Midland Rd, London, NW1 1AT, United Kingdom.

The application of genomics to medicine has accelerated the discovery of mutations underlying disease and enhanced our knowledge of the molecular underpinnings of diverse pathologies. As the amount of human genetic material queried via sequencing has grown exponentially in recent years, so too has the number of rare variants observed. Despite progress, our ability to distinguish which rare variants have clinical significance remains limited. Read More

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CTRP13 Protects H9c2 Cells Against Hypoxia/Reoxygenation (H/R)-Induced Injury Via Regulating the AMPK/Nrf2/ARE Signaling Pathway.

Cell Transplant 2021 Jan-Dec;30:9636897211033275

Department of Cardiology, Kaifeng People's Hospital, Kaifeng 475000, China.

Myocardial infarction (MI) is identified as the myocardial necrosis due to myocardial ischemia/reperfusion (I/R) injury and remains a leading cause of mortality. C1q/TNF-related protein 13 (CTRP13) is a member of CTRP family that has been found to be involved in coronary artery disease (CAD). However, the role of CTRP13 in MI remains unclear. Read More

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Biomechanics of adjacent segment after three-level lumbar fusion, hybrid single-level semi-rigid fixation with two-level lumbar fusion.

Comput Methods Biomech Biomed Engin 2021 Aug 2:1-9. Epub 2021 Aug 2.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, P. R. China.

Multi-level spinal fusion has been reported in some cases to lead to adjacent segment disease (ASD) and proximal junctional kyphosis (PJK). The purpose of this study was to demonstrate a polyether-ether-ketone (PEEK) rod fixation system implanted adjacent to a two-level lumbar fusion would have a lower risk of PJK than three-level lumbar fusion, which was investigated by comparing the biomechanical effects on the adjacent level after surgical procedures. Four finite element (FE) models of the lumbar-sacral spine (intact model (INT), L4-S1 fusion model (L4-S1 FUS), L3-S1 fusion model (L3-S1 FUS), and single-level PEEK rod semi-rigid fixation adjacent to L4-S1 fusion model (FUSPRF)) were established. Read More

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Single nucleotide polymorphisms within the loci: another potential source of clinically important genetic variation for ?

Jeremy S Brown

Infect Immun 2021 Aug 2:IAI0037421. Epub 2021 Aug 2.

Centre for Inflammation and Tissue Repair, UCL Respiratory, University College London, London, United Kingdom.

The capsule is essential for disease pathogenesis, suggesting that even minor genetic changes within the locus could potentially have important consequences. Arends et al. have identified 79 different non-synonymous SNPs in the locus of 338 19A serotype strains, and shown significant variations between strains in nucleotide sugars content and capsule shedding. Read More

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HLA genetic polymorphism in patients with Coronavirus Disease 2019 in Midwestern United States.

HLA 2021 Aug 2. Epub 2021 Aug 2.

Division of Laboratory and Genomic Medicine, Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.

The experience of individuals with Coronavirus Disease 2019 (COVID-19) ranges from asymptomatic to life threatening multi-organ dysfunction. Specific HLA alleles may affect the predisposition to severe COVID-19 due to their role in presenting viral peptides to launch the adaptive immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this population-based case-control study in the midwestern United States, we performed high-resolution HLA typing of 234 cases hospitalized for COVID-19 in the St. Read More

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Plasma proteins, cognitive decline, and 20-year risk of dementia in the Whitehall II and Atherosclerosis Risk in Communities studies.

Alzheimers Dement 2021 Aug 2. Epub 2021 Aug 2.

Department of Epidemiology and Public Health, University College London, London, UK.

Introduction: Plasma proteins affect biological processes and are common drug targets but their role in the development of Alzheimer's disease and related dementias remains unclear. We examined associations between 4953 plasma proteins and cognitive decline and risk of dementia in two cohort studies with 20-year follow-ups.

Methods: In the Whitehall II prospective cohort study proteins were measured using SOMAscan technology. Read More

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Functional dyspepsia: A critical appraisal of the European consensus from a global perspective.

Neurogastroenterol Motil 2021 Aug 1:e14216. Epub 2021 Aug 1.

Faculty of Health and Medicine, University of Newcastle, Newcastle, NSW, Australia.

The European Society for Neurogastroenterology and Motility's (ESNM) consensus document on functional dyspepsia (FD) has provided a critical review of the evidence for 36 statements highly relevant to the diagnosis and treatment of FD. We provide here our expert opinion on the North American, Australian and Asian assessments of the same topics discussed, and provide evidence where the ESNM consensus may deviate from those in the other continents. New and exciting data about the pathogenesis of FD are emerging including the possible central role of duodenal micro-inflammation (most notably eosinophilic duodenitis and intestinal mast cell disease), but this is a very dynamic field with several gaps remaining in our understanding that are summarized in this review. Read More

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[Driving Cessation and Mental Health in Persons With Dementia: A Preliminary Study].

Hu Li Za Zhi 2021 Aug;68(4):43-52

PhD, RN, Associate Professor, School of Gerontology Health Management, College of Nursing, Taipei Medical University, Taiwan, ROC.

Background: The driving safety of persons with dementia (PwD) is an important public-health issue worldwide. Driving is closely related to personal autonomy, self-esteem, and independence. When PwD lose their driving privileges, this may lead to negative effects on mental health. Read More

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Flow-dependent regulation of endothelial Tie2 by GATA3 in vivo.

Intensive Care Med Exp 2021 Aug 2;9(1):38. Epub 2021 Aug 2.

Department of Nephrology and Hypertension, Hannover Medical School, Hannover, Germany.

Background: Reduced endothelial Tie2 expression occurs in diverse experimental models of critical illness, and experimental Tie2 suppression is sufficient to increase spontaneous vascular permeability. Looking for a common denominator among different critical illnesses that could drive the same Tie2 suppressive (thereby leak inducing) phenotype, we identified "circulatory shock" as a shared feature and postulated a flow-dependency of Tie2 gene expression in a GATA3 dependent manner. Here, we analyzed if this mechanism of flow-regulation of gene expression exists in vivo in the absence of inflammation. Read More

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Degree of Preservation of Neurovascular Bundles in Radical Prostatectomy and Recurrence of Prostate Cancer.

Eur Urol Open Sci 2021 Aug 19;30:25-33. Epub 2021 Jun 19.

Department of Urology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Background: Reports on possible benefits for continence with nerve-sparing (NS) radical prostatectomy have expanded the indications beyond preservation of erectile function. It is unclear whether NS surgery affects oncological outcomes.

Objective: To determine whether the degree of NS during radical prostatectomy influences oncological outcomes. Read More

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WATER versus WATER II 2-Year Update: Comparing Aquablation Therapy for Benign Prostatic Hyperplasia in 30-80-cm and 80-150-cm Prostates.

Eur Urol Open Sci 2021 Mar 31;25:21-28. Epub 2021 Jan 31.

Division of Urology, Centre Hospitalier de l'Université de Montréal, Montreal, Canada.

Background: Surgical options are limited when treating large (>80 cm) prostates for lower urinary tract symptoms (LUTS) due to benign prostatic hyperplasia (BPH). Open simple prostatectomy remains the most common procedure performed for large prostates. There is a need for novel surgical approaches with shorter learning curves and effective treatment. Read More

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Salvage Magnetic Resonance Imaging-guided Transurethral Ultrasound Ablation for Localized Radiorecurrent Prostate Cancer: 12-Month Functional and Oncological Results.

Eur Urol Open Sci 2020 Dec 25;22:79-87. Epub 2020 Nov 25.

Department of Urology, University of Turku and Turku University Hospital, Turku, Finland.

Background: Up to half of all men who undergo primary radiotherapy for localized prostate cancer (PCa) experience local recurrence.

Objective: To evaluate the safety and early functional and oncological outcomes of salvage magnetic resonance imaging-guided transurethral ultrasound ablation (sTULSA) for men with localized radiorecurrent PCa.

Design Setting And Participants: This prospective, single-center phase 1 study (NCT03350529) enrolled men with biopsy-proven localized PCa recurrence after radiotherapy. Read More

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December 2020

A single cell transcriptomics map of paracrine networks in the intrinsic cardiac nervous system.

iScience 2021 Jul 19;24(7):102713. Epub 2021 Jul 19.

Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA.

We developed a spatially-tracked single neuron transcriptomics map of an intrinsic cardiac ganglion, the right atrial ganglionic plexus (RAGP) that is a critical mediator of sinoatrial node (SAN) activity. This 3D representation of RAGP used neuronal tracing to extensively map the spatial distribution of the subset of neurons that project to the SAN. RNA-seq of laser capture microdissected neurons revealed a distinct composition of RAGP neurons compared to the central nervous system and a surprising finding that cholinergic and catecholaminergic markers are coexpressed, suggesting multipotential phenotypes that can drive neuroplasticity within RAGP. Read More

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A comprehensive tool box for large animal studies of intervertebral disc degeneration.

JOR Spine 2021 Jun 14;4(2):e1162. Epub 2021 Jun 14.

Department of Clinical Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands.

Preclinical studies involving large animal models aim to recapitulate the clinical situation as much as possible and bridge the gap from benchtop to bedside. To date, studies investigating intervertebral disc (IVD) degeneration and regeneration in large animal models have utilized a wide spectrum of methodologies for outcome evaluation. This paper aims to consolidate available knowledge, expertise, and experience in large animal preclinical models of IVD degeneration to create a comprehensive tool box of anatomical and functional outcomes. Read More

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Subjective cognitive decline higher among sexual and gender minorities in the United States, 2015-2018.

Alzheimers Dement (N Y) 2021 28;7(1):e12197. Epub 2021 Jul 28.

Division of Population Health National Center for Chronic Disease Prevention and Health Promotion Centers for Disease Control and Prevention Atlanta Georgia USA.

Introduction: Subjective cognitive decline (SCD) represents self-reported problems with memory, a possible early sign of dementia. Little is known about SCD among sexual and gender minority (SGM) adults who identify as lesbian, gay, bisexual, and/or transgender or gender non-binary.

Methods: Data were weighted to represent population estimates from 25 states' 2015-2018 Behavioral Risk Factor Surveillance System to describe SCD in adults ≥45 years by SGM status. Read More

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Study of the G Protein Nucleolar 2 Value in Liver Hepatocellular Carcinoma Treatment and Prognosis.

Biomed Res Int 2021 19;2021:4873678. Epub 2021 Jul 19.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Key Lab of Glycoconjugate Research, Ministry of Public Health, Shanghai 200032, China.

LIHC (liver hepatocellular carcinoma) mostly occurs in patients with chronic liver disease. It is primarily induced by a vicious cycle of liver injury, inflammation, and regeneration that usually last for decades. The G protein nucleolar 2 (GNL2), as a protein-encoding gene, is also known as NGP1, Nog2, Nug2, Ngp-1, and HUMAUANTIG. Read More

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A Genome-Wide Association Study of Age-Related Hearing Impairment in Middle- and Old-Aged Chinese Twins.

Biomed Res Int 2021 17;2021:3629624. Epub 2021 Jul 17.

Department of Epidemiology and Health Statistics, Public Health College, Qingdao University, No. 38 Dengzhou Road, Shibei District, Qingdao, 266021 Shandong Province, China.

Background: Age-related hearing impairment (ARHI) is considered an unpreventable disorder. We aimed to detect specific genetic variants that are potentially related to ARHI via genome-wide association study (GWAS).

Methods: A sample of 131 dizygotic twins was genotyped for single-nucleotide polymorphism- (SNP-) based GWAS. Read More

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Enhanced Detection of -Specific T Cells in Experimentally-Infected Cattle.

Front Vet Sci 2021 14;8:676710. Epub 2021 Jul 14.

Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, United States.

Bovine tuberculosis (bTB), caused by infection with , continues to be a major economic burden associated with production losses and a public health concern due to its zoonotic nature. As with other intracellular pathogens, cell-mediated immunity plays an important role in the control of infection. Characterization of such responses is important for understanding the immune status of the host, and to identify mechanisms of protective immunity or immunopathology. Read More

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