13,747 results match your criteria concentration detergent

Good Old-Fashioned Protein Concentration Determination by Amino Acid Analysis.

Methods Mol Biol 2021 ;2228:21-28

Medizinisches Proteom-Center (MPC), Medical Faculty, Ruhr-University Bochum, Bochum, Germany.

Although a lot of new methods for protein concentration determination have been developed and established the last years, the amino acid analysis has still this relevance within proteomics for multiple reasons especially in the quantitative protein analysis. Amino acid analysis enables indirectly both the protein and peptide concentration determination which are essential for using the same amounts for comparative quantitative experiments. Moreover, the quantity and quality of synthetic peptides can be verified. Read More

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January 2021

Effect of Detergents and Osmolytes on Thermal Stability of Native and Mutant Rhodobacter sphaeroides Reaction Centers.

Biochemistry (Mosc) 2021 Apr;86(4):517-524

Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.

Photosynthetic reaction center (RC) of the purple bacterium Rhodobacter sphaeroides is one of the most well-studied transmembrane pigment-protein complexes. It is a relatively stable protein with established conditions for its isolation from membranes, purification, and storage. However, it has been shown that some amino acid substitutions can affect stability of the RC, which results in a decrease of the RCs yield during its isolation and purification, disturbs spectral properties of the RCs during storage, and can lead to sample heterogeneity. Read More

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Replacing Synperonic® N in the Physical Developer fingermark visualisation process: Reformulation.

Forensic Sci Int 2021 Apr 10;323:110786. Epub 2021 Apr 10.

Home Office Centre for Applied Science and Technology (CAST), Sandridge AL4 9HQ, UK.

The Physical Developer solution currently recommended for use in the United Kingdom for fingermark visualisation uses two surfactants: n-dodecylamine acetate (nDDAA) and Synperonic® N. Synperonic® N is covered by the EU directive 82/242/EEC, which sought to phase out chemicals with degradation products more harmful than their precursor. This study explores the replacement of Synperonic® N with alternative detergents and examines their ability to produce clear, stable solutions that are effective at developing fingermarks. Read More

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Development and Validation of Equations for Predicting the Metabolizable Energy Value of Double-Low Rapeseed Cake for Growing Pigs.

Animals (Basel) 2021 Apr 19;11(4). Epub 2021 Apr 19.

Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China.

The study was conducted to develop and validate an equation to predict the metabolizable energy (ME) of double-low rapeseed cakes (DLRSC) for growing pigs based on their chemical compositions. In Experiment 1, 66 growing pigs (initial body weight 36.6 ± 4. Read More

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Effects of feeding a live yeast on rumen fermentation and fiber degradability of tropical and subtropical forages.

J Sci Food Agric 2021 Apr 28. Epub 2021 Apr 28.

State University of Maringá, Maringá, Brazil, 87020900.

Background: The effect of live yeast Saccharomyces cerevisiae strain CNCM I-1077 (SC) on the ruminal degradability of different forages commonly found in dairy diets in South America was evaluated. We also assessed if SC supplementation interacts with forage group to affect ruminal fiber degradability. Four non-lactating rumen-cannulated Holstein cows were randomly assigned to two treatment sequences: Control-SC-Control or SC-Control-SC, in a switchback design, with three 30-d periods. Read More

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Detection of the residual concentration of sodium dodecyl sulfate in the decellularized whole rabbit kidney extracellular matrix.

Cell Tissue Bank 2021 Apr 28. Epub 2021 Apr 28.

Pediatric Urology and Regenerative Medicine Research Center, Pediatric Center of Excellence, Department of Pediatric Urology, Children's Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharibs Street, Keshavarz Boulevard, 1419733151, Tehran, Iran.

To optimize rabbit kidney decellularization protocol, using sodium dodecyl sulfate (SDS) as a commonly used detergent, a methylene blue based assay was employed for detecting the minimum nontoxic SDS level for future cell seeding. The rabbit kidney tissues were decellularized with the perfusion-based method and underwent several investigations to determine the efficacy of decellularization in preserving the extracellular matrix (ECM) and cell removal. SDS detection was performed by incubating with methylene blue and subsequent extraction with chloroform. Read More

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Self-Organized Amphiphiles Are Poor Hydroxyl Radical Scavengers in Fast Photochemical Oxidation of Proteins Experiments.

J Am Soc Mass Spectrom 2021 May 21;32(5):1155-1161. Epub 2021 Apr 21.

Department of Biomolecular Sciences, University of Mississippi, University, Mississippi 38677, United States.

Analysis of membrane protein topography using fast photochemical oxidation of proteins (FPOP) has been reported in recent years but is still underrepresented in literature. Based on the hydroxyl radical reactivity of lipids and other amphiphiles, it is believed that the membrane environment acts as a hydroxyl radical scavenger decreasing effective hydroxyl radical doses and resulting in less observed oxidation of proteins. We found no significant change in bulk solvent radical scavenging activity upon the addition of disrupted cellular membranes up to 25600 cells/μL using an inline radical dosimeter. Read More

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Effects of different detergent-containing children's toothpastes on the viability, osteogenic and chondrogenic differentiation of human dental periodontal ligament stem cells and gingival stem cells in vitro.

Tissue Cell 2021 Mar 26;72:101538. Epub 2021 Mar 26.

Istanbul University, Faculty of Dentistry, Department of Pedodontics, Istanbul, Turkey. Electronic address:

Background: Detergents are the most commonly used compounds in toothpastes due to their foaming and cleaning peoperties. This study aimed to investigate the effects of children's toothpastes with different detergent content on the viability, the osteogenic and chondrogenic differentiation potentials of human mesenchymal stem cells.

Methods: The necessary tissues for human periodontal ligament mesenchymal stem cells (hPDLMSCs) and human gingival mesenchymal stem cells (hGMSCs) isolation were obtained during extraction of 10 impacted third molar teeth. Read More

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Aqueous Phase Behavior of a NaLAS-Polycarboxylate Polymer System.

Langmuir 2021 May 20;37(17):5099-5108. Epub 2021 Apr 20.

School of Chemical and Process Engineering, University of Leeds, Leeds LS2 9JT, United Kingdom.

Linear alkylbenzene sulfonate (NaLAS) surfactant is often combined with polycarboxylate polymers in detergent formulations. However, the behavior of these aqueous surfactant-polymer systems in the absence of an added electrolyte is unreported. This work investigates the behavior of such systems using polarized light microscopy, small-angle X-ray scattering (SAXS), centrifugation, and H NMR techniques. Read More

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Micro-mechanical properties of corneal scaffolds from two different bio-models obtained by an efficient chemical decellularization.

J Mech Behav Biomed Mater 2021 Apr 16;119:104510. Epub 2021 Apr 16.

Rheology, Nanofluids and Microfluidics Laboratory, Autonomous University of the State of Mexico, Faculty of Sciences, Campus Universitario ''El Cerrillo, Piedras Blancas", Carretera Toluca-Ixtlahuaca km 15.5, C.P. 50200 Estado de México, Mexico. Electronic address:

The present study elucidates the impact of detergent-based chemical decellularization on the micro-mechanical properties of porcine and rabbit corneas for the purpose of extracellular matrix (ECM) derived scaffolds. Aiming to optimize the decellularization process, different concentrations of Sodium Dodecyl Sulfate (SDS), Triton X-100 and CHAPS detergents were assessed on their ability to decellularize corneas from both bio-models at incubation periods of 12 and 24h. We evaluated the effect of decellularization on corneal ECM Young's Modulus and various area's roughness parameters (topography features) at a microscale by using Atomic Force Microscopy (AFM). Read More

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Influence of amount and frequency of protein supplementation to ruminants consuming low-quality cool-season forages: Efficiency of nitrogen utilization in lambs and performance of gestating beef cows.

J Anim Sci 2021 Apr 19. Epub 2021 Apr 19.

Department of Animal Science, Texas A&M University, College Station, TX, USA.

We evaluated the influence of amount and crude protein (CP) supplementation frequency (SF) on nitrogen (N) use by wethers and performance of late-gestation beef cows. In Exp. 1, 7 Western whiteface wethers (31. Read More

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Effects of corn grain endosperm type and conservation method on feed intake, feeding behavior, and productive performance of lactating dairy cows.

J Dairy Sci 2021 Apr 14. Epub 2021 Apr 14.

Department of Animal Science, Michigan State University, East Lansing 48824.

Our objective was to evaluate the effects of corn grain varying in endosperm type and conserved as high-moisture or dry ground corn on dry matter intake (DMI), feeding behavior, ruminal fermentation, and yields of milk and milk components of cows in early to mid-lactation. Seven ruminally and duodenally cannulated Holstein cows (73 ± 39 d in milk; mean ± SD) were used in a duplicated 4 × 4 Latin square design with 21-d periods. A 2 × 2 factorial arrangement of treatments was used with main effects of corn grain endosperm type (floury or vitreous) conserved as high-moisture corn (HMC) or dry ground corn (DGC). Read More

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Feeding dairy cows bakery by-products enhanced nutrient digestibility, but affected fecal microbial composition and pH in a dose-dependent manner.

J Dairy Sci 2021 Apr 14. Epub 2021 Apr 14.

Department for Farm Animals and Veterinary Public Health, Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. Electronic address:

We reported recently that adding bakery by-products (BP) to the diets of dairy cows up to 30% improved performance and rumen pH, but caused major shifts in the nutrient profile and availability, likely modifying nutrient degradation patterns throughout the gastrointestinal tract. The aim of this study was to investigate the effects of the gradual replacement of cereals by BP on the apparent total-tract digestibility (ATTD), the fermentation patterns, and the microbial community in feces of dairy cows. Twenty-four mid-lactating Simmental cows (149 ± 22. Read More

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Effects of corn grain endosperm type and fineness of grind on feed intake, feeding behavior, and productive performance of lactating dairy cows.

J Dairy Sci 2021 Apr 14. Epub 2021 Apr 14.

Department of Animal Science, Michigan State University, East Lansing 48824.

Our objective was to evaluate effects of corn grain endosperm type and fineness of grind on feed intake, feeding behavior, ruminal fermentation, and productive performance of lactating cows. Eight ruminally and duodenally cannulated Holstein cows in mid lactation (130 ± 42 d in milk; mean ± standard deviation) were used in a duplicated 4 × 4 Latin square design with 21-d periods. A 2 × 2 factorial arrangement of treatments was used with main effects of corn grain endosperm type (floury or vitreous) and fineness of grind of corn grain (fine or medium). Read More

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Effective decellularisation of human saphenous veins for biocompatible arterial tissue engineering applications: Bench optimisation and feasibility in vivo testing.

J Tissue Eng 2021 Jan-Dec;12:2041731420987529. Epub 2021 Mar 29.

Bristol Heart Insitute and Translational Biomedical Research Centre, Translational Health Sciences, Bristol Medical School, University of Bristol, Bristol Royal Infirmary, Bristol, UK.

Human saphenous vein (hSV) and synthetic grafts are commonly used conduits in vascular grafting, despite high failure rates. Decellularising hSVs (D-hSVs) to produce vascular scaffolds might be an effective alternative. We assessed the effectiveness of a detergent-based method using 0% to 1% sodium dodecyl sulphate (SDS) to decellularise hSV. Read More

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Validation of direct boiling method for simple and efficient genomic DNA extraction and PCR-based macroalgal species determination.

J Phycol 2021 Apr 9. Epub 2021 Apr 9.

Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon, 22012, Korea.

A rapid, simple, and cost-effective total DNA extraction method referred to as a direct boiling method for PCR-based algal species determination was validated using representative species of green, brown and red algae. Each sample was briefly minced in two buffers, Tris-EDTA (TE) or PCR buffer, and transferred to a heat block for boiling at 98°C for 5 min. No detergent was used in this experiment. Read More

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Bio-cleaning Efficiency of Rhamnolipids Produced from Native Pseudomonas aeruginosa Grown on Agro-industrial By-products for Liquid Detergent Formulation.

Appl Biochem Biotechnol 2021 Apr 7. Epub 2021 Apr 7.

Botany Department, Faculty of Science, Suez Canal University, P.O. Box: 41522, Ismailia, Egypt.

The cleaning activity of surface-active agents such as rhamnolipids (RLs) requires utmost effectiveness and is employed abundantly in various industries, particularly laundry cleaning, detergents, and cosmetics. In the current study, RLs were produced from Pseudomonas aeruginosa isolated from oil-contaminated soil using a minimal medium amended with agro-industrial by-products of refinery vegetable oil wastes (comprising of unsaturated types of fatty acids as determined by GC analysis) and dairy whey. The results showed that an amount of 5. Read More

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induction and characterisation of tetraploid drumstick tree ( Lam.).

Open Life Sci 2020 18;15(1):840-847. Epub 2020 Nov 18.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangzhou, 510642, China.

Artificial induction of polyploidy is widely used in breeding programmes to improve the agronomic traits. The drumstick tree ( Lam.) has a range of potential commercial uses, as the vegetative organs have high nutritional, medicinal, and feed values. Read More

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November 2020

Conditions stimulating neutral detergent fiber degradation by dosing branched-chain volatile fatty acids. I: Comparison with branched-chain amino acids and forage source in ruminal batch cultures.

J Dairy Sci 2021 Apr 1. Epub 2021 Apr 1.

Department of Animal Sciences, The Ohio State University, Columbus 43210. Electronic address:

Three experiments assessed branched-chain volatile fatty acid (BCVFA) stimulation of neutral detergent fiber (NDF) disappearance after 24 h of incubation in batch cultures derived from ruminal fluid inocula that were enriched with particulate-phase bacteria. In experiment 1, a control was compared with 3 treatments with isomolar doses of all 3 BCVFA (plus valerate), all 3 branched-chain AA (BCAA), or half of each BCVFA and BCAA mix with either alfalfa or grass hays (50%) and ground corn grain (50%). A portion of the BCAA and BCVFA doses were enriched with C, and valerate (also enriched with C) was added with BCVFA. Read More

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Comparison of the effects of short-term feeding of sodium acetate and sodium bicarbonate on milk fat production.

J Dairy Sci 2021 Apr 1. Epub 2021 Apr 1.

Department of Animal Science, Pennsylvania State University, University Park 16802. Electronic address:

Supplementation with sodium acetate (NaAcet) increases milk fat production through an apparent stimulation of de novo lipogenesis in the mammary gland. Sodium acetate increases acetate supply to the mammary gland, but it also increases dietary cation-anion difference, which can also increase milk fat yield. The objective of this study was to determine if the effect of NaAcet on milk fat production was due to an increase in acetate supply or an increase in dietary cation-anion difference. Read More

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Effects of a xylanase-rich enzyme on intake, milk production, and digestibility of dairy cows fed a diet containing a high proportion of bermudagrass silage.

J Dairy Sci 2021 Apr 1. Epub 2021 Apr 1.

Department of Animal Sciences, University of Florida, Gainesville 32611. Electronic address:

We previously reported that milk production in dairy cows was increased by adding a specific xylanase-rich exogenous fibrolytic enzyme (XYL) to a total mixed ration (TMR) containing 10% bermudagrass silage (BMD). Two follow-up experiments were conducted to examine whether adding XYL would increase the performance of dairy cows consuming a TMR containing a higher (20%) proportion of BMD (Experiment 1) and to evaluate the effects of XYL on in vitro fermentation and degradability of the corn silage, BMD, and TMR (Experiment 2). In Experiment 1, 40 lactating Holstein cows in early lactation (16 multiparous and 24 primiparous; 21 ± 3 d in milk; 589 ± 73 kg of body weight) were blocked by milk yield and parity and randomly assigned to the Control and XYL treatments. Read More

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Water intake and ingestive behavior of sheep fed diets based on silages of cactus pear and tropical forages.

Trop Anim Health Prod 2021 Apr 4;53(2):244. Epub 2021 Apr 4.

Universidade Federal do Vale do São Francisco, Petrolina, PE, 56304-917, Brazil.

The objective was to evaluate the water intake and ingestive behavior of sheep fed diets containing silages of cactus pear combined with tropical forages. Forty sheep without defined breed, intact, with initial average weight of 22.65 ± 1. Read More

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Antenna Protein Clustering In Vitro Unveiled by Fluorescence Correlation Spectroscopy.

Int J Mol Sci 2021 Mar 15;22(6). Epub 2021 Mar 15.

Centre Algatech, Institute of Microbiology of the Czech Academy of Sciences, Opatovický Mlýn, 379 81 Třeboň, Czech Republic.

Antenna protein aggregation is one of the principal mechanisms considered effective in protecting phototrophs against high light damage. Commonly, it is induced, in vitro, by decreasing detergent concentration and pH of a solution of purified antennas; the resulting reduction in fluorescence emission is considered to be representative of non-photochemical quenching in vivo. However, little is known about the actual size and organization of antenna particles formed by this means, and hence the physiological relevance of this experimental approach is questionable. Read More

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Issues of Feeding Strategy for Lactating Cows in Vietnamese Smallholder Dairy Farms.

Animals (Basel) 2021 Mar 8;11(3). Epub 2021 Mar 8.

School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia.

A limited literature suggests relatively simple feeding regimes and diet formulation strategies for dairy cows in Vietnamese smallholder dairy farms (SDFs). This study aimed to classify and compare feeding regimes and nutrient balance for lactating cows between four typical dairy regions (south lowland, south highland, north lowland, and north highland) in Vietnam and evaluate the possibility of systematic dietary imbalance. Eight SDFs from each of the four regions were visited for two adjacent milking periods per farm. Read More

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Comparing Approaches to Normalize, Quantify, and Characterize Urinary Extracellular Vesicles.

J Am Soc Nephrol 2021 May 29;32(5):1210-1226. Epub 2021 Mar 29.

Division of Nephrology and Transplantation, Department of Internal Medicine, Erasmus Medical Center, University Medical Center Rotterdam, Rotterdam, The Netherlands

Background: Urinary extracellular vesicles (uEVs) are a promising source for biomarker discovery, but optimal approaches for normalization, quantification, and characterization in spot urines are unclear.

Methods: Urine samples were analyzed in a water-loading study, from healthy subjects and patients with kidney disease. Urine particles were quantified in whole urine using nanoparticle tracking analysis (NTA), time-resolved fluorescence immunoassay (TR-FIA), and EVQuant, a novel method quantifying particles gel immobilization. Read More

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Effects of ground, steam-flaked, and super-conditioned corn grain on production performance and total-tract digestibility of dairy cows.

J Dairy Sci 2021 Mar 24. Epub 2021 Mar 24.

Innovation Center, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran.

This study aimed to evaluate the effects of feeding ground, steam-flaked, or super-conditioned corn on production performance, rumen fermentation, nutrient digestibility, and milk fatty acid (FA) profile of lactating dairy cows. Twenty-four lactating Holstein cows (130 ± 12 d in milk) in a completely randomized block design experiment were assigned to 1 of 3 treatments that contained 31% of one of the following corn types: (1) ground corn; (2) steam-flaked corn; and (3) super-conditioned corn. Actual milk yield was greater in the super-conditioned corn diet than in the steam-flaked and ground corn diets. Read More

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Effects of milk replacer feeding rate and fat content on Jersey calf nutrient digestion and performance to 4 months of age.

J Dairy Sci 2021 Mar 24. Epub 2021 Mar 24.

Nurture Research Center, Provimi North America, Cargill Animal Nutrition, Brookville, OH 45309.

This study evaluated effects of milk replacer (MR) feeding rate and fat concentration in MR on total-tract digestion (TTD) and growth performance in Jersey calves. Jersey heifer calves (n = 100, 2 blocks of 50; initially 30 ± 3.0 kg of body weight; 4-11 d of age) were randomly assigned to 1 of 4 treatments in a 2 × 2 factorial arrangement of MR feeding rate [454 g for 42 d, and 227 g for 7 d (MOD); or 454 g for 7 d, 681 g for 35 d, and 341 g for 7 d (HI), as-fed basis] and MR fat content (17 or 24% fat as-fed basis). Read More

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Optimization, purification, and biochemical characterization of thermoalkaliphilic lipase from a novel Geobacillus stearothermophilus FMR12 for detergent formulations.

Int J Biol Macromol 2021 Mar 22;181:125-135. Epub 2021 Mar 22.

Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box: 21934, Alexandria, Egypt. Electronic address:

This study was aimed to produce a high compatible thermoalkaliphilic lipase (TA) with detergents from new thermophilic bacterial strains utilizing fish wastes for industrial application. Among bacterial isolates, a new Geobacillus stearothermophilus FMR12 efficiently utilized fish wastes at a concentration of 20% (w/v), exhibiting highly lipolytic activity at extreme thermal and alkaline pH conditions. Optimized fermentation parameters of TA lipase production were ascertained, promoting the productivity of the TA lipase from 424 to 1038 U/ml. Read More

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Comparison between the effects of feeding copper sulphate-treated and untreated rapeseed cake containing high glucosinolates on rumen fermentation, nutrient digestion and nitrogen metabolism in steers.

J Anim Physiol Anim Nutr (Berl) 2021 Mar 25. Epub 2021 Mar 25.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Two trials were carried out to study the effects of copper sulphate (CuSO ) on detoxifying glucosinolates (GLS) in rapeseed cake (RSC) and compare the effects of feeding CuSO -treated and untreated RSC on nutrient digestion and nitrogen (N) metabolism in steers. In Trial 1, different concentrations of CuSO solution (1.6 vs. Read More

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Genotoxicity induced by nerol, an essential oil present in citric plants using human peripheral blood mononuclear cells (PBMC) and HepG2/C3A cells as a model.

J Toxicol Environ Health A 2021 Jun 24;84(12):518-528. Epub 2021 Mar 24.

Speech and Hearing Therapy Department, São Paulo State University - UNESP - Faculty of Philosophy and Sciences, Marília, Brazil.

Nerol (-3,7-dimethyl-2,6-octadien-1-ol) is a monoterpene widely used in cosmetic products, household detergents and cleaners, as well as a flavoring in several food products. Despite the high level of human exposure to nerol, an absence of studies regarding potential genetic toxicity in human cells exists. The aim of this investigation was to examine the cytotoxic and genotoxic potential of this monoterpene on human peripheral blood mononuclear cells as well as hepatic metabolizing HepG2/C3A human cell line. Read More

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