Cell Tissue Bank 2021 Apr 28. Epub 2021 Apr 28.
Pediatric Urology and Regenerative Medicine Research Center, Pediatric Center of Excellence, Department of Pediatric Urology, Children's Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharibs Street, Keshavarz Boulevard, 1419733151, Tehran, Iran.
To optimize rabbit kidney decellularization protocol, using sodium dodecyl sulfate (SDS) as a commonly used detergent, a methylene blue based assay was employed for detecting the minimum nontoxic SDS level for future cell seeding. The rabbit kidney tissues were decellularized with the perfusion-based method and underwent several investigations to determine the efficacy of decellularization in preserving the extracellular matrix (ECM) and cell removal. SDS detection was performed by incubating with methylene blue and subsequent extraction with chloroform. Read More