16,186 results match your criteria concentration assayed

Cytocompatible cellulose nanofibers from invasive plant species Agave americana L. and Ricinus communis L.: a renewable green source of highly crystalline nanocellulose.

J Zhejiang Univ Sci B 2021 Jun;22(6):450-461

CQM‒Centro de Química da Madeira, MMRG, Universidade da Madeira, Campus Universitário da Penteada, 9020-105 Funchal, Portugal.

In this study, the fibers of invasive species L. and L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers. Read More

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High risk of early sub-therapeutic penicillin concentrations after intramuscular benzathine penicillin G injections in Ethiopian children and adults with rheumatic heart disease.

PLoS Negl Trop Dis 2021 Jun 11;15(6):e0009399. Epub 2021 Jun 11.

Faculty of Health and Medical Sciences, University of Western Australia, Perth, Western Australia, Australia.

Introduction: Intramuscular benzathine penicillin G (BPG) injections are a cornerstone of secondary prophylaxis to prevent acute rheumatic fever (ARF) and rheumatic heart disease (RHD). Uncertainties regarding inter-ethnic and preparation variability, and target exposure profiles of BPG injection are key knowledge gaps for RHD control.

Methods: To evaluate BPG pharmacokinetics (PK) in patients receiving 4-weekly doses in Ethiopia, we conducted a prospective cohort study of ARF/RHD patients attending cardiology outpatient clinics. Read More

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Beam pen lithography as a new tool for spatially controlled photochemistry, and its utilization in the synthesis of multivalent glycan arrays.

Chem Sci 2014 May 4;5(5):2023-2030. Epub 2014 Feb 4.

Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA.

Herein, we describe how cantilever-free scanning probes can be used to deposit precursor material and subsequently irradiate the precursor to initiate polymerization, resulting in a 3D lithographic method wherein the position, height and diameter of each feature can be tuned independently. Specifically, acrylate and methacrylate monomers were patterned onto thiol terminated glass and subsequently exposed to UV light produced brush polymers by a photoinduced radical acrylate polymerization reaction. Here, we report the first examples of glycan arrays, comprised of methacrylate brush polymers that are side-chain functionalized with α-glucose, by this new lithographic approach. Read More

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Multivariable analysis of the influence of cross-reactive carbohydrate determinant inhibition and other factors on intradermal and serological allergen test results: a prospective, multicentre study.

Vet Dermatol 2021 Jun 9. Epub 2021 Jun 9.

Department of Clinical Sciences and Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, 3900 Spruce Street, Philadelphia, PA, 19104, USA.

Background: Serological allergen testing (SAT) is used widely to formulate allergen-specific immunotherapy for atopic dogs. Serum immunoglobulin (Ig)E specific for cross-reactive carbohydrate determinants (CCD) can produce false-positive reactions, creating discrepancy between SAT and intradermal allergen test (IDAT) results.

Objectives: The primary objective was to determine if inhibition of anti-CCD IgE in a commercial assay improved correlation with IDAT. Read More

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MiR-613 Promotes Cell Death in Breast Cancer Cells by Downregulation of Nicotinamide Phosphoribosyltransferase and Reduction of NAD.

DNA Cell Biol 2021 Jun 8. Epub 2021 Jun 8.

Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.

NAD is mainly biosynthesized by the enzymatic action of nicotinamide phosphoribosyltransferase (NAMPT) through the salvage pathway. NAD is indispensable for the proper function and metabolism of all living cells, including cancer cells. Our previous researches revealed that inhibition of by miRNA (miR) could suppress NAD levels and thereby hinder the growth and promotion of breast cancer (BC). Read More

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Multi-organ protective effect of on low concentration toxic metal mixture in albino rats.

Int J Physiol Pathophysiol Pharmacol 2021 15;13(2):52-68. Epub 2021 Apr 15.

African Centre of Excellence for Public Health and Toxicological Research (ACE-PUTOR), University of Port Harcourt PMB, 5323 Port Harcourt, Choba, Nigeria.

Heavy metal mixture can induce multiple organ damage through oxidative stress and inflammatory processes. Dietary intervention using natural antidotes in resource poor countries where classical metal chelators are either not affordable or available can be explored as an alternative means of management of public health effects of chronic heavy metal exposure. The search for natural antidote against the deleterious effects of heavy metals gives the thrust for this study. Read More

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Circulating microRNA-762 upregulates colorectal cancer may be accompanied by Wnt-1/β-catenin signaling.

Cancer Biomark 2021 May 31. Epub 2021 May 31.

Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan.

Colorectal cancer (CRC) has become the third most common cause of cancer-related deaths. CRC occurs because of abnormal growth of cells that can invade other tissues and cause distant metastases. Researchers have suggested that aberrant microRNA (miRNA) expression is involved in the initiation and progression of cancers. Read More

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Removal of overpainting from an historical painting of the XVIII Century: A yeast enzymatic approach.

J Biotechnol 2021 Jun 4;335:55-64. Epub 2021 Jun 4.

Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovakia; Caravella, s.r.o., Tupolevova 2, 851 01 Bratislava, Slovakia. Electronic address:

Biocleaning of cultural heritage items is mainly performed using living microorganisms. Approaches utilizing the enzymes of isolated microorganisms have not been frequently investigated. To find an enzymatic alternative for the removal of an oil-based overpainting, we focused on the characterization and use of a yeast Extracellular Enzymatic Mixture (EEM). Read More

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The endocrine disruptor 17β-trenbolone alters the relationship between pre- and post-copulatory sexual traits in male mosquitofish (Gambusia holbrooki).

Sci Total Environ 2021 May 26;790:148028. Epub 2021 May 26.

School of Biological Sciences, Monash University, Victoria, Australia.

It is now well-established that reproduction in wildlife can be disrupted by anthropogenic environmental changes, such as chemical pollution. However, very little is known about how these pollutants might affect the interplay between pre- and post-copulatory mechanisms of sexual selection. Here, we investigated the impacts of 21-day exposure of male eastern mosquitofish (Gambusia holbrooki) to a field-realistic level (average measured concentration: 11 ng/L) of the endocrine-disrupting chemical 17β-trenbolone (17β-TB) on pre- and post-copulatory reproductive traits. Read More

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Efficient extra-mitochondrial aerobic ATP synthesis in neuronal membrane systems.

J Neurosci Res 2021 Jun 3. Epub 2021 Jun 3.

Department of Pharmacy, Biochemistry Lab., University of Genoa, Genoa, Italy.

The nervous system displays high energy consumption, apparently not fulfilled by mitochondria, which are underrepresented therein. The oxidative phosphorylation (OxPhos) activity, a mitochondrial process that aerobically provides ATP, has also been reported also in the myelin sheath and the rod outer segment (OS) disks. Thus, commonalities and differences between the extra-mitochondrial and mitochondrial aerobic metabolism were evaluated in bovine isolated myelin (IM), rod OS, and mitochondria-enriched fractions (MIT). Read More

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Comparative efficacy of commercially available deoxynivalenol detoxifying feed additives on growth performance, total tract digestibility of components, and physiological responses in nursery pigs fed diets formulated with naturally contaminated corn.

Transl Anim Sci 2021 Apr 10;5(2):txab050. Epub 2021 Mar 10.

Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.

Comparative efficacy of deoxynivalenol (DON) detoxifying feed additives (FA) was evaluated in growth performance (exp. 1) and apparent total tract digestibility (ATTD; exp. 2) nursery pig studies. Read More

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Scopolamine analysis in beverages: Bicolorimetric device vs portable nano liquid chromatography.

Talanta 2021 Sep 20;232:122406. Epub 2021 Apr 20.

MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain. Electronic address:

Scopolamine (SCP) is often involved in sexual assaults and robberies, particularly in recreational environments. Therefore, analytical tools are required for the analysis of this compound amenable for the field. In this work, a sensor for SCP is described based on the entrapment of KMnO into polydimethylsiloxane (PDMS). Read More

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September 2021

A Red-Berry Mixture as a Nutraceutical: Detailed Composition and Neuronal Protective Effect.

Molecules 2021 May 27;26(11). Epub 2021 May 27.

Departamento de Metabolismo y Nutrición, Instituto de Ciencia y Tecnología de Alimentos y Nutrición (ICTAN-CSIC), C/José Antonio Nováis, 10, 28040 Madrid, Spain.

Recommendations towards increased consumption of fresh fruit and vegetables are well supported by epidemiological and clinical trials. However, in some specific cases, it is difficult to follow these recommendations and the use of nutraceuticals or, in the present work, a freeze-dried fruits mixture can be recommended in order to afford the optimal consumption of dietary polyphenols naturally present in fruits and vegetables. In this work we have carefully characterized a red-berry mixture in terms of polyphenol composition, encountering mainly anthocyanins, which account for a total of 2. Read More

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Diclofenac N-Derivatives as Therapeutic Agents with Anti-Inflammatory and Anti-Cancer Effect.

Int J Mol Sci 2021 May 11;22(10). Epub 2021 May 11.

Department of Organic Chemistry, Institute of Biotechnology, University of Granada, 18071 Granada, Spain.

A series of diclofenac N-derivatives (, , , , , -) were synthesized in order to test their anti-cancer and anti-inflammatory effects. The anticarcinogen activity has been assayed against three cancer cell lines: HT29, human colon cancer cells; Hep-G2, human hepatic cells; and B16-F10, murine melanoma cells. First, we determined the cytotoxicity of the different compounds, finding that the most effective compound was compound against all cell lines and both compounds and in human Hep-G2 and HT29 cell lines. Read More

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Melatonin Treatment to Pomegranate Trees Enhances Fruit Bioactive Compounds and Quality Traits at Harvest and during Postharvest Storage.

Antioxidants (Basel) 2021 May 21;10(6). Epub 2021 May 21.

Department of Applied Biology, EPSO, University Miguel Hernández. Ctra. Beniel km. 3.2, 03312 Orihuela, Alicante, Spain.

The effect of melatonin pomegranate tree treatments on fruit quality and bioactive compounds with antioxidant activity at harvest and during storage at 10 °C for 60 days was assayed in two consecutive years, 2019 and 2020. In the first year, trees were treated with 0.1, 0. Read More

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Folic acid alleviates the blood brain barrier permeability and oxidative stress and prevents cognitive decline in sepsis-surviving rats.

Microvasc Res 2021 Sep 29;137:104193. Epub 2021 May 29.

Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Graduate Program in Health Sciences, University of South Santa Catarina, Tubarão, SC, Brazil. Electronic address:

Sepsis is a complication of an infection which imbalance the normal regulation of several organ systems, including the central nervous system (CNS). Evidence points towards inflammation and oxidative stress as major steps associated with brain dysfunction in sepsis. Thus, we investigated the folic acid (FA) effect as an important antioxidant compound on acute brain dysfunction in rats and long term cognitive impairment and survival. Read More

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September 2021

Optimization of culture conditions for enhanced productions in mixotrophic culture.

Prep Biochem Biotechnol 2021 May 31:1-9. Epub 2021 May 31.

Department of Food Science and Technology, Islamic Azad University, Sabzevar Branch, Sabzevar, Iran.

() is a green microalga known for its tendency to produce lipid and β-carotene. Fatty acid profile, lipid and β-carotene productions of the microalga cultivated under different mixotrophic conditions were assayed. Notably, in spite of a broad spectrum of substrates served, mixotrophic cultivations slightly affected the fatty acid composition, and as a result C16:0 and C18:0, C18:1, C18:2 and C18:3 were identified as main fatty acids. Read More

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Facile Cloud Point Extraction for the Separation and Determination of Phenolic Acids from Dandelion.

ACS Omega 2021 May 17;6(20):13508-13515. Epub 2021 May 17.

Academy of Chinese Medicine Science, Henan University of Chinese Medicine, Zhengzhou 450046, China.

It is significantly crucial to develop a robust pretreatment for the quantitative analysis of herbs. However, the traditional strategies are time-consuming, tedious, and not eco-friendly. In this work, cloud point extraction (CPE) is engineered for the simultaneous separation and enrichment of ferulic acid (FA), chlorogenic acid (CLA), and caffeic acid (CA) from dandelion prior to its determination by high-performance liquid chromatography (HPLC). Read More

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Screening for patients with Gaucher's disease using routine pathology results: PATHFINDER (ferritin, alkaline phosphatase, platelets) study.

Int J Clin Pract 2021 May 30:e14422. Epub 2021 May 30.

Centogene GmbH, Rostock, Germany.

Aims: Lysosomal β-glucocerebrosidase A (GBA) deficiency causes Gaucher disease (GD), a recessive disorder caused by bi-allelic mutations in GBA. The prevalence of GD is associated with ethnicity but largely unknown and potentially underestimated in many countries. GD may manifest with organomegaly, bone involvement, and neurological symptoms as well as abnormal laboratory biomarkers. Read More

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Genetic toxicity testing using human in vitro organotypic airway cultures: Assessing DNA damage with the CometChip and mutagenesis by Duplex Sequencing.

Environ Mol Mutagen 2021 Jun 14;62(5):306-318. Epub 2021 Jun 14.

U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, Arkansas, USA.

The organotypic human air-liquid-interface (ALI) airway tissue model has been used as an in vitro cell culture system for evaluating the toxicity of inhaled substances. ALI airway cultures are highly differentiated, which has made it challenging to evaluate genetic toxicology endpoints. In the current study, we assayed DNA damage with the high-throughput CometChip assay and quantified mutagenesis with Duplex Sequencing, an error-corrected next-generation sequencing method capable of detecting a single mutation per 10 base pairs. Read More

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Factors affecting the accuracy and reliability of the measurement of anti-Müllerian hormone concentration in the clinic.

J Int Med Res 2021 May;49(5):3000605211016161

Reproductive Medicine Center, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.

Objective: We aimed to identify the factors that influence serum anti-Müllerian hormone (AMH) concentration measurements.

Methods: We collected serum samples between May and September 2018 and compared the effect on AMH concentration measured by ELISA of conditions including venepuncture, storage time, storage temperature, locations of the reaction microplate, and the use of the oral contraceptive pill and gonadotrophin-releasing hormone (GnRH).

Results: AMH concentration was not affected by food intake but was affected by haemolysis. Read More

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Bile acid synthesis, modulation, and dementia: A metabolomic, transcriptomic, and pharmacoepidemiologic study.

PLoS Med 2021 May 27;18(5):e1003615. Epub 2021 May 27.

Clinical and Translational Neuroscience Section, Laboratory of Behavioral Neuroscience, National Institute on Aging (NIA), National Institutes of Health (NIH), Baltimore, Maryland, United States of America.

Background: While Alzheimer disease (AD) and vascular dementia (VaD) may be accelerated by hypercholesterolemia, the mechanisms underlying this association are unclear. We tested whether dysregulation of cholesterol catabolism, through its conversion to primary bile acids (BAs), was associated with dementia pathogenesis.

Methods And Findings: We used a 3-step study design to examine the role of the primary BAs, cholic acid (CA), and chenodeoxycholic acid (CDCA) as well as their principal biosynthetic precursor, 7α-hydroxycholesterol (7α-OHC), in dementia. Read More

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Assessment of combining biosynthesized silver nanoparticles using and gamma irradiation for controlling (saunders) (lepidoptera: Gelechiidae).

Int J Radiat Biol 2021 Jun 10:1-17. Epub 2021 Jun 10.

Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Cairo, Egypt.

Purpose: Combining gamma irradiation and nanotechnology has become one of the most promising new approaches for area-wide (AW) pest management in recent years. The laboratory trials were conducted to determine the combining effects of -AgNPs and gamma irradiation for controlling Radio-sensitivity of male pupae at different doses of gamma radiation and the effectiveness of biosynthesized silver nanoparticles using on larval instar were assayed. Additionally, the ultrastructure changes on the alimentary canal of 4 instar larvae were studied to evaluate the impact of the combined approach at a cellular level. Read More

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In silico and in vitro inhibitory potential of an organometallic Cu (II) complex on Leishmania major stages.

Ann Parasitol 2021 ;67(1):45-54

Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Leishmaniosis results in a serious complication, principally in the tropical and subtropical areas. Metalcored complexes, like meglumine antimoniate (MA) have proven antileishmanial activity. Similarly, in this research, we investigated the effects of Cu (II) dimethoxy bipyridine (CuDMOBP) against Leishmania major stages in silico and in vitro. Read More

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Key Aroma Compounds in Roasted White Kołuda Goose.

J Agric Food Chem 2021 Jun 21;69(21):5986-5996. Epub 2021 May 21.

Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland.

Aroma-active compounds in the roasted leg meat of White Kołuda goose were assayed by gas chromatography-olfactometry, using aroma extract dilution analysis and solvent-assisted flavor evaporation. Quantitation, recombination-omission tests, and sensory evaluation were carried out. Thirty aroma compounds, for which odor activity values (OAVs) were calculated and for which the flavor dilution factors were greater than or equal to 1, were identified. Read More

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Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads.

Appl Biochem Biotechnol 2021 May 21. Epub 2021 May 21.

Departamento de Biocatálisis, Instituto de Catálisis-CSIC, C/ Marie Curie 2, Campus UAM-CSI, Cantoblanco, 28049, Madrid, Spain.

Tris is an extensively used buffer that presents a primary amine group on its structure. In the present work trypsin, chymotrypsin and penicillin G acylase (PGA) were immobilized/stabilized on glyoxyl agarose in presence of different concentrations of Tris (from 0 to 20 mM). The effects of the presence of Tris during immobilization were studied analyzing the thermal stability of the obtained immobilized biocatalysts. Read More

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Socioeconomic status, financial stress, and glucocorticoid resistance among youth with asthma: Testing the moderation effects of maternal involvement and warmth.

Brain Behav Immun 2021 May 17. Epub 2021 May 17.

Department of Psychology, Wayne State University, United States; Department of Family Medicine and Public Health Sciences, Wayne State University, United States. Electronic address:

Objectives: Children who grow up in more socioeconomically disadvantaged homes experience greater levels of inflammation and worse asthma symptoms than children from more advantaged families. However, recent evidence suggests that certain family-level factors can mitigate health disparities associated with socioeconomic status (SES). In a sample of youth with asthma, we investigated the potential buffering effects of maternal involvement and warmth on SES disparities in asthma-related immune responses, assessed via glucocorticoid resistance (GR) of immune cells. Read More

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Comparison of different preparation techniques of dried blood spot quality controls in newborn screening for congenital adrenal hyperplasia.

PLoS One 2021 20;16(5):e0252091. Epub 2021 May 20.

Metabolic and Newborn Screening Laboratory, Department of Pediatrics, University of Szeged, Szeged, Hungary.

In newborn screening, samples suspected for congenital adrenal hyperplasia (CAH), a potentially lethal inborn error of steroid biosynthesis, need to be confirmed using liquid chromatography-tandem mass spectrometry. Daily quality controls (QCs) for the 2nd-tier CAH assay are not commercially available and are therefore generally prepared within the laboratory. For the first time, we aimed to compare five different QC preparation approaches used in routine diagnostics for CAH on the concentrations of cortisol, 21-deoxycortisol, 11-deoxycortisol, 4-androstenedione and 17-hydroxyprogesterone in dried blood spots. Read More

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Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid Reactions.

Int J Nanomedicine 2021 12;16:3317-3327. Epub 2021 May 12.

Department of Pharmacology, Health Science Center, Xi'an Jiaotong University, Xi'an, People's Republic of China.

Objective: Multivitamins containing Tween 80 can cause anaphylactoid reactions. The objective of this study was to develop a new lipid emulsion containing 13 fat- and water-soluble vitamins for injection (13V-LE) that were simultaneously dissolved in one bottle and to evaluate the stability of and anaphylactoid reactions to 13V-LE.

Methods: Particle size, ζ-potential, and polydispersity of 13V-LE were assayed with a Zetasizer Nano ZS. Read More

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Predictive Performance of Population Pharmacokinetic Models of Levetiracetam in Children and Evaluation of Dosing Regimen.

J Clin Pharmacol 2021 May 16. Epub 2021 May 16.

Service de Pharmacologie Clinique, Hôpital Cochin, APHP, Paris, France.

Levetiracetam is a broad-spectrum antiepileptic drug that exhibits high interindividual variability in serum concentrations in children. A population pharmacokinetic approach can be used to explain this variability and optimize dosing schemes. The objectives are to identify the best predictive population pharmacokinetic model for children and to evaluate recommended doses using simulations and Bayesian forecasting. Read More

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