10,676 results match your criteria complexes apoptosis

Synthesis, characterization of ruthenium(II), nickel(II), palladium(II), and platinum(II) triphenylphosphine-based complexes bearing an ONS-donor chelating agent: Interaction with biomolecules, antioxidant, in vitro cytotoxic, apoptotic activity and cell cycle analysis.

J Inorg Biochem 2021 Jul 21;223:111549. Epub 2021 Jul 21.

Chemistry Department, Faculty of Science, Damietta University, New Damietta 34517, Egypt. Electronic address:

Four new transition metal complexes, [M(PPh)(L)]CHOH (M = Ni(II) (1), Pd(II) (2)) [Pt (PPh)(HL)]Cl (3) and [Ru(CO)(PPh)(L)] (4) (HL = 2,4-dihydroxybenzaldehyde-S-methyldithiocarbazate, PPh = triphenylphosphine) have been synthesized and characterized by elemental analyses (C, H, N), FTIR, NMR (H, P), ESI-MS and UV-visible spectroscopy. The molecular structure of (1) and (2) complexes was confirmed by single-crystal X-ray crystallography. It showed a distorted square planar geometry for both complexes around the metal center, and the HL adopt a bi-negative tridentate chelating mode. Read More

View Article and Full-Text PDF

Development of a multi-target anticancer Sn(II) pyridine-2-carboxaldehyde thiosemicarbazone complex.

Dalton Trans 2021 Jul 27. Epub 2021 Jul 27.

State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, Guangxi, China.

In this study, we proposed to design effective multi-target anticancer agents based on the chelation of nontoxic metals with ligands that possess anticancer activity. In total, five Sn(ii) pyridine-2-carboxaldehyde thiosemicarbazone complexes are synthesized and their activities are tested. Among these complexes, C5 is found to show the highest cytotoxicity on investigating their structure-activity relationships. Read More

View Article and Full-Text PDF

Ligands and DNA in the allosteric control of retinoid receptors function.

Essays Biochem 2021 Jul 23. Epub 2021 Jul 23.

Centre de Biologie Structurale (CBS), Inserm, CNRS, Univ Montpellier, Montpellier, France.

Retinoids are a family of compounds that include both vitamin A (all-trans retinol) and its naturally occurring metabolites such as retinoic acids (e.g. all-trans retinoic acid) as well as synthetic analogs. Read More

View Article and Full-Text PDF

VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, induces cell cycle arrest and apoptosis in breast cancer cells by inhibiting the Wnt/β-catenin pathway.

Sci Rep 2021 Jul 22;11(1):14985. Epub 2021 Jul 22.

Division of Oncology, Gansu Provincial Hospital, 204 Donggang West road, Chengguan district, Lanzhou City, 730000, Gansu Province, China.

Schiff base compounds and their metal complexes have become important synthetic organic drugs due to their extensive biological activities, which include anticancer, antibacterial and antiviral effects. In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced by VALD-3. Read More

View Article and Full-Text PDF

Iridium(III)-BBIP complexes induce apoptosis via PI3K/AKT/mTOR pathway and inhibit A549 lung tumor growth in vivo.

J Inorg Biochem 2021 Jul 21;223:111550. Epub 2021 Jul 21.

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China. Electronic address:

The new ligand BBIP (BBIP = 2-(7-bromo-2H-benzo[d]imidazole-4-yl)-1H-imidazo[4,5-f][1,10]phenanthroline) with its iridium(III) complexes: [Ir(ppy)(BBIP)](PF) (ppy = 2-phenylpyridine, Ir1), [Ir(bzq)(BBIP)](PF) (bzq = benzo[h]quinolone, Ir2) and [Ir(piq)(BBIP)](PF) (piq = 1-phenylisoquinoline, Ir3) were synthesized and characterized by elemental analysis, High Resolution Mass Spectrometer (HRMS), H NMR and C{1H} NMR. The cytotoxicity of the complexes against A549, HepG2, SGC-7901, BEL-7402, HeLa and normal LO2 was evaluated through 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) method. The results show that Ir1 exhibits high cytotoxic activity against A549 cells with a low IC value of 4. Read More

View Article and Full-Text PDF

Mitotropic Triphenylphosphonium Doxorubicin-Loaded Core-Shell Nanoparticles for Cellular and Mitochondrial Sequential Targeting of Breast Cancer.

Int J Pharm 2021 Jul 23:120936. Epub 2021 Jul 23.

Nanomedicine Labs, Center for Materials Science (CMS), Zewail City of Science and Technology, 6 of October City, 12578, Giza, Egypt. Electronic address:

Hypotheses Targeted therapy exploits cancerous niches' properties including acidic extracellular environment, hypoxic tumor core, and tumor-specific surface antigens. The present study aims to develop and evaluate a sequential targeted core-shell nanoparticulate (NPs) system for treatment of breast cancer. Sequential (double-stage) targeting was achieved at the cellular-level employing the selective CD44- receptor binding hyaluronic acid (HA), followed by subcellular mitochondrial drug-delivery using a synthesized triphenylphosphonium-conjugated doxorubicin (DOX-TPP). Read More

View Article and Full-Text PDF

Copper(II) Complexes Containing Natural Flavonoid Pomiferin Show Considerable In Vitro Cytotoxicity and Anti-inflammatory Effects.

Int J Mol Sci 2021 Jul 16;22(14). Epub 2021 Jul 16.

Department of Cell Biology and Genetics, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic.

A series of new heteroleptic copper(II) complexes of the composition [Cu(L)(bpy)]NO·2MeOH (), [Cu(L)(dimebpy)]NO·2HO (), [Cu(L)(phen)]NO·2MeOH (), [Cu(L)(bphen)]NO·MeOH (), [Cu(L)(dppz)]NO·MeOH () was prepared, where HL = 3-(3,4-dihydroxyphenyl)-5-hydroxy-8,8-dimethyl-6-(3-methylbut-2-ene-1-yl)-4,8-benzo[1,2-:3,4-']dipyran-4-one, (pomiferin) and bpy = 2,2'-bipyridine, dimebpy = 4,4'-dimethyl-2,2'-bipyridine, phen = 1,10-phenanthroline, bphen = 4,7-diphenyl-1,10-phenanthroline, and dppz = dipyrido[3,2-:2',3'-]phenazine. The complexes were characterized using elemental analysis, infrared and Uis spectroscopies, mass spectrometry, thermal analysis and conductivity measurements. The in vitro cytotoxicity, screened against eight human cancer cell lines (breast adenocarcinoma (MCF-7), osteosarcoma (HOS), lung adenocarcinoma (A549), prostate adenocarcinoma (PC-3), ovarian carcinoma (A2780), cisplatin-resistant ovarian carcinoma (A2780R), colorectal adenocarcinoma (Caco-2) and monocytic leukemia (THP-1), revealed the complexes as effective antiproliferative agents, with the IC values of 2. Read More

View Article and Full-Text PDF

Ruthenium(II) and Platinum(II) Complexes with Biologically Active Aminoflavone Ligands Exhibit In Vitro Anticancer Activity.

Int J Mol Sci 2021 Jul 15;22(14). Epub 2021 Jul 15.

Departament of Bioinorganic Chemistry, Medical University of Lodz, Muszyńskiego 1, 90-151 Lodz, Poland.

Continuing our studies on the mechanisms underlying the cytotoxicity of potential drugs, we have described several aspects of the in vitro anticancer activity of ruthenium(II) and platinum(II) complexes with bioactive, synthetic aminoflavone ligands. We examined the mechanism of proapoptotic activity of -dichlorobis(3-imino-2-methoxyflavanone)ruthenium(II), -dichlorobis(3-imino-2-ethoxyflavanone)ruthenium(II), and -dichlorobis(3-aminoflavone)platinum(II). Cisplatin was used as a reference compound. Read More

View Article and Full-Text PDF

A Comparative Analysis of the In Vitro Anticancer Activity of Iridium(III) {η-CMeR} Complexes with Variable R Groups.

Int J Mol Sci 2021 Jul 10;22(14). Epub 2021 Jul 10.

Department of Chemistry and Industrial Chemistry, University of Pisa, Via Moruzzi 13, I-56124 Pisa, Italy.

Piano-stool iridium complexes based on the pentamethylcyclopentadienyl ligand (Cp*) have been intensively investigated as anticancer drug candidates and hold much promise in this setting. A systematic study aimed at outlining the effect of Cp* mono-derivatization on the antiproliferative activity is presented here. Thus, the dinuclear complexes [Ir(η-CMeR)Cl(μ-Cl)] (R = Me, ; R = H, ; R = Pr, ; R = 4-CHF, ; R = 4-CHOH, ), their 2-phenylpyridyl mononuclear derivatives [Ir(η-CMeR)(k,kPhPy)Cl] (), and the dimethylsulfoxide complex [Ir{η-CMe(4-CHOH)}Cl(κ-MeS=O)] () were synthesized, structurally characterized, and assessed for their cytotoxicity towards a panel of six human and rodent cancer cell lines (mouse melanoma, B16; rat glioma, C6; breast adenocarcinoma, MCF-7; colorectal carcinoma, SW620 and HCT116; ovarian carcinoma, A2780) and one primary, human fetal lung fibroblast cell line (MRC5). Read More

View Article and Full-Text PDF

Design, Hemiysnthesis, crystal structure and anticancer activity of 1, 2, 3-triazoles derivatives of totarol.

Bioorg Chem 2021 Jul 14;115:105165. Epub 2021 Jul 14.

Laboratoire de Synthèse Organique et Physico-Chimie Moléculaire, Département de Chimie, Faculté des Sciences, Semlalia, Université Cadi-Ayyad, B.P 2390, Marrakech 40001, Morocco. Electronic address:

A new series of diverse triazoles linked to the hydroxyl group of totarol were synthesized using click chemistry approach. The structures of these compounds were elucidated by HRMS, IR and NMR spectroscopy. The structure of compound 3 g was also confirmed by x-ray single crystal diffraction. Read More

View Article and Full-Text PDF

Developmental competence of heat stressed oocytes from Holstein and Limousine cows matured in vitro.

Reprod Domest Anim 2021 Jul 21. Epub 2021 Jul 21.

Department of Obstetrics and Reproduction, Veterinary Faculty, University of Thessaly, Karditsa, Greece.

The negative effects of heat stress on dairy cattle's fertility have been extensively studied, but the relevant knowledge for beef cattle is rather limited. The aims of this study were to investigate the effects of HS during in vitro maturation on the developmental potential of oocytes derived from Limousine and Holstein cows and to estimate the effect of the differential gene expression of important genes in oocytes, cumulus cells, and blastocysts in the growth competence between the breeds. In 7 replicates, cumulus oocyte complexes from Holstein and Limousine cows were matured for 24 hours at 39°C (controls C; Hol_39, Lim_39) or at 41°C from hour 2 to hour 8 of IVM (treated T; Hol_41, Lim_41), fertilized, and presumptive zygotes were cultured for 9 days at 39°C. Read More

View Article and Full-Text PDF

A novel microphysiological colon platform to decipher mechanisms driving human intestinal permeability.

Cell Mol Gastroenterol Hepatol 2021 Jul 17. Epub 2021 Jul 17.

Emulate Inc., 27 Drydock Avenue, Boston, MA, 02210, USA. Electronic address:

Background & Aims: The limited availability of organoid systems that mimic the molecular signatures and architecture of human intestinal epithelium has been an impediment to allowing them to be harnessed for the development of therapeutics as well as physiological insights. We developed a microphysiological Organ-on-Chip platform designed to mimic properties of human intestinal epithelium leading to insights into barrier integrity.

Methods: We combined the human biopsy-derived LGR5+ organoids and Organ-on-Chip technologies to establish a micro-engineered human Colon Intestine-Chip. Read More

View Article and Full-Text PDF

Synthesis, Characterization, DNA/HSA Interactions, and Anticancer Activity of Two Novel Copper(II) Complexes with 4-Chloro-3-Nitrobenzoic Acid Ligand.

Molecules 2021 Jul 1;26(13). Epub 2021 Jul 1.

School of Chemical and Biological Engineering, Guangxi Normal University for Nationalities, 23 Fozi Road, Chongzuo 532200, China.

The purpose of this study was to identify new metal-based anticancer drugs; to this end, we synthesized two new copper(II) complexes, namely [Cu(ncba)(phen)] () and [Cu(ncba)(bpy)] (), comprised 4-chloro-3-nitrobenzoic acid as the main ligand. The single-crystal XRD approach was employed to determine the copper(II) complex structures. Binding between these complexes and calf thymus DNA (CT-DNA) and human serum albumin (HSA) was explored by electronic absorption, fluorescence spectroscopy, and viscometry. Read More

View Article and Full-Text PDF

MicroRNAs Involved in Oxidative Stress Processes Regulating Physiological and Pathological Responses.

Microrna 2021 Jul 16. Epub 2021 Jul 16.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science/ Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China.

Oxidative stress influences several physiological and pathological cellular events, including cell differentiation, excessive growth, proliferation, apoptosis, and the inflammatory response. Therefore, oxidative stress is involved in the pathogenesis of various diseases, including pulmonary fibrosis, epilepsy, hypertension, atherosclerosis, Parkinson's disease, cardiovascular disease, and Alzheimer's disease. Recent studies have shown that several microRNAs (miRNAs) are involved in developing various diseases caused by oxidative stress and that miRNAs may be helpful to determine the inflammatory characteristics of immune responses during infection and disease. Read More

View Article and Full-Text PDF

[Corrigendum] Cryptotanshinone inhibits IgE‑mediated degranulation through inhibition of spleen tyrosine kinase and tyrosine‑protein kinase phosphorylation in mast cells.

Mol Med Rep 2021 Sep 19;24(3). Epub 2021 Jul 19.

Department of Biological Science, Sookmyung Women's University, Seoul 04310, Republic of Korea.

Following the publication of the above article, an interested reader drew to the authors' attention that they had mentioned that activated PKCδ phosphorylates IKKβ in order that IKKβ is relocated to the plasma membrane, resulting in the induction of mast cell degranulation; however, four references the authors had included did not seem to support this statement. The authors have re-examined their paper, and realized that the four references the reader mentioned were indeed cited incorrectly, and wish to rectify this error through revising the third paragraph in the Discussion section, the References section, and an associated figure (Fig. 6C) in order to avoid any further misunderstandings on the part of the readership. Read More

View Article and Full-Text PDF
September 2021

Direct embryotoxicity of chromium (III) exposure during preimplantation development.

J Reprod Dev 2021 Jul 18. Epub 2021 Jul 18.

Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs; National Engineering Laboratory for Animal Breeding; College of Animal Science and Technology, China Agricultural University, Beijing, China.

Chromium in its trivalent form (chromium (III)) is an essential component of a balanced diet, and its deficiency disturbs glucose and lipid metabolism in humans and animals. The prevailing view is that chromium (III) is notably less toxic than chromium (VI), which is genotoxic and carcinogenic. Thus, the biotransformation of environmental chromium (VI) to chromium (III) is a promising and environmentally friendly detoxification method. Read More

View Article and Full-Text PDF

Phosphorescent rhenium(I) complexes conjugated with artesunate: Mitochondrial targeting and apoptosis-ferroptosis dual induction.

J Inorg Biochem 2021 Jul 9;223:111537. Epub 2021 Jul 9.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China. Electronic address:

Cell death is essential for cancer, which can be induced through multiple mechanisms. Ferroptosis, a newly emerging form of non-apoptotic cell death, involves the generation of iron-dependent reactive oxygen species (ROS). In this study, we designed and synthesized two artesunate (ART) conjugated phosphorescent rhenium(I) complexes (Re(I)-ART conjugates), [Re(N^N)(CO)(PyCHOART)](PF) (Re-ART-1 and Re-ART-2) (Py = pyridine, N^N = 1,10-phenanthroline (phen, in Re-ART-1) and 4,7-diphenyl-1,10-phenanthroline (DIP, in Re-ART-2)) that can specifically locate in the mitochondria of human cervical carcinoma (HeLa). Read More

View Article and Full-Text PDF

Osteosarcoma stem cell potent gallium(III)-polypyridyl complexes bearing diflunisal.

Chemistry 2021 Jul 16. Epub 2021 Jul 16.

University of Leicester, Chemistry, University Road, LE1 7RH, Leicester, UNITED KINGDOM.

We report the anti-osteosarcoma stem cell (OSC) properties of a series of gallium(III)-polypyridyl complexes (5-7) containing diflunisal, a non-steroidal anti-inflammatory drug. The most effective complex within the series, 6 (containing 3,4,7,8-tetramethyl-1,10-phenanthroline) displayed similar potency towards bulk osteosarcoma cells and OSCs, in the nanomolar range. Remarkably, 6 exhibited significantly higher monolayer and sarcosphere OSC potency (up to three orders of magnitude) than clinically approved drugs used in frontline (cisplatin and doxorubicin) and secondary (etoposide, ifosfamide, and carboplatin) osteosarcoma treatments. Read More

View Article and Full-Text PDF

Aqueous extracts of induce S-phase arrest and apoptosis in human melanoma A375 cells.

Oncol Lett 2021 Aug 30;22(2):628. Epub 2021 Jun 30.

Institute of Sericultural and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, P.R. China.

, also called 'Sanghuang' mushroom in Chinese, has various medicinal uses, but its effects on human melanoma cells have not been reported. The present study investigated the inhibitory ability and potential anticancer mechanism of the aqueous extracts of (SH). The results revealed that SH inhibited the proliferation of A375 human melanoma cells in a dose-dependent manner, and flow cytometry analysis suggested that SH induced A375 cell cycle arrest at S phase and apoptosis. Read More

View Article and Full-Text PDF

Deletion of RBMX RGG/RG motif in Shashi-XLID syndrome leads to aberrant p53 activation and neuronal differentiation defects.

Cell Rep 2021 Jul;36(2):109337

Segal Cancer Center, Lady Davis Institute for Medical Research and Gerald Bronfman Department of Oncology, and Departments of Biochemistry, Human Genetics, and Medicine, McGill University, Montréal, QC H3T 1E2, Canada; Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada. Electronic address:

RNA-binding proteins play important roles in X-linked intellectual disability (XLID). In this study, we investigate the contribution of the XLID-associated RBMX in neuronal differentiation. We show that RBMX-depleted cells exhibit aberrant activation of the p53 pathway. Read More

View Article and Full-Text PDF

Half-lantern Cyclometalated Platinum(II) Complexes as Anticancer Agents: Molecular docking, Apoptosis, Cell Cycle Analysis and Cytotoxic Activity Evaluations.

Anticancer Agents Med Chem 2021 Jul 12. Epub 2021 Jul 12.

Toxicology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Background And Objective: In the design of modern metal-based anticancer drugs, platinum-based complexes have gained growing interest. In this study, the anticancer activity of half-lantern cyclometalated Pt(II)‒Pt(II) complexes were was evaluated using MTT, apoptosis, cell cycle analysis, and DNA binding studies.

Materials And Methods: The cytotoxicity of Pt(II)‒Pt(II) complexes were evaluated against different cancer cell lines such as human lung (A549), breast (MCF-7, and MDA-MB-231), ovarian (SKOV-3), and colon (HT-29) as well as normal breast (MCF-10A), and human lung fibroblast MRC-5 cells using MTT assay. Read More

View Article and Full-Text PDF

[Proteomic analysis of lacrimal gland adenoid cystic carcinoma with high-grade transformation].

Zhonghua Yan Ke Za Zhi 2021 Jul;57(7):531-539

Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin Branch of National Clinical Research Center for Ocular Disease, Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin 300384, China.

To analyze the protein expression differences of lacrimal gland adenoid cystic carcinoma (LACC) with high-grade transformation (HGT). Experimental study. A total of 8 paraffin tissue samples were collected in Tianjin Medical University Eye Hospital from December 2012 to January 2019. Read More

View Article and Full-Text PDF

N-acetyl-cysteine and the control of oxidative stress during in vitro ovarian follicle growth, oocyte maturation, embryo development and cryopreservation.

Anim Reprod Sci 2021 Jul 6;231:106801. Epub 2021 Jul 6.

Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Comandante Maurocélio Rocha Ponte 100, CEP 62041-040, Sobral, CE, Brazil. Electronic address:

Oxidative stress is generated by an imbalance between reactive oxygen species (ROS) formation and cellular defense mechanisms. To reduce cellular damage caused by ROS in vivo or in vitro, N-acetyl-cysteine (NAC) is converted into metabolites that have the capacity of stimulating synthesis of glutathione (GSH) which functions directly as free radical scavengers. The NAC antioxidant potential evaluated to the greatest extent is the indirect action of NAC, as a precursor of GSH, with glutathione being the primary antioxidant in cells. Read More

View Article and Full-Text PDF

The BRD4 inhibitor JQ1 promotes melanoma cell apoptosis by regulating mitochondrial dynamics.

Cancer Sci 2021 Jul 12. Epub 2021 Jul 12.

Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun, China.

Although the role of bromodomain-containing protein 4 (BRD4) in ovarian cancer, pancreatic cancer, multiple lymphoma and many other diseases is well known, its function in cutaneous melanoma is only partially understood. Here, our results show that the BRD4 inhibitor JQ1 promotes the apoptosis of B16 melanoma cells by altering mitochondrial dynamics, thereby inducing mitochondrial dysfunction and increasing oxidative stress. We found that treatment of B16 cells with different concentrations of JQ1 (125 nM or 250 nM), significantly downregulated the expression of protein subunits involved in mitochondrial respiratory chain complexes I, III, IV, and V, increased reactive oxygen species (ROS), induced energy metabolism dysfunction, significantly enhanced apoptosis and activated the mitochondrial apoptosis pathway. Read More

View Article and Full-Text PDF

Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves and Fertility.

Front Cell Dev Biol 2021 24;9:660165. Epub 2021 Jun 24.

Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina.

Pregnancy rates in ewes are markedly low after cervical insemination with frozen-thawed sperm. Sensitivity of ram sperm to freeze-thawing is related to the lipid composition of the membrane, particularly to its low sterol content. Recently, we proved that sterol content of ram sperm can be increased by treatment with methyl-β-cyclodextrin-sterol complexes and we provided mechanistic based evidence on the differential behavior of cholesterol and desmosterol in the ram sperm membrane. Read More

View Article and Full-Text PDF

A Novel Ruthenium(II) Complex With Lapachol Induces G2/M Phase Arrest Through Aurora-B Kinase Down-Regulation and ROS-Mediated Apoptosis in Human Prostate Adenocarcinoma Cells.

Front Oncol 2021 24;11:682968. Epub 2021 Jun 24.

School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Brazil.

Lapachol is a well-studied natural product that has been receiving great interest due to its anticancer properties that target oxidative stress. In the present work, two novel lapachol-containing ruthenium(II) complexes [Ru(Lap)(dppm)(bipy)]PF () and [Ru(Lap)(dppm)(phen)]PF () [Lap = lapachol, dppm = 1,1'-bis(diphosphino)methane, bipy = 2,2'-bipyridine, phen = 1,10-phenantroline] were synthesized, fully characterized, and investigated for their cellular and molecular responses on cancer cell lines. We found that both complexes exhibited a potent cytotoxic effect in a panel of cancer cell lines in monolayer cultures, as well as in a 3D model of multicellular spheroids formed from DU-145 human prostate adenocarcinoma cells. Read More

View Article and Full-Text PDF

Endothelial Immunity Trained by Coronavirus Infections, DAMP Stimulations and Regulated by Anti-Oxidant NRF2 May Contribute to Inflammations, Myelopoiesis, COVID-19 Cytokine Storms and Thromboembolism.

Front Immunol 2021 25;12:653110. Epub 2021 Jun 25.

Centers of Cardiovascular Research, Inflammation, Translational & Clinical Lung Research, Temple University Lewis Katz School of Medicine, Philadelphia, PA, United States.

To characterize transcriptomic changes in endothelial cells (ECs) infected by coronaviruses, and stimulated by DAMPs, the expressions of 1311 innate immune regulatomic genes (IGs) were examined in 28 EC microarray datasets with 7 monocyte datasets as controls. We made the following findings: The majority of IGs are upregulated in the first 12 hours post-infection (PI), and maintained until 48 hours PI in human microvascular EC infected by middle east respiratory syndrome-coronavirus (MERS-CoV) (an EC model for COVID-19). The expressions of IGs are modulated in 21 human EC transcriptomic datasets by various PAMPs/DAMPs, including LPS, LPC, shear stress, hyperlipidemia and oxLDL. Read More

View Article and Full-Text PDF

Therapeutic Role of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Female Reproductive Diseases.

Front Endocrinol (Lausanne) 2021 23;12:665645. Epub 2021 Jun 23.

Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Reproductive disorders, including intrauterine adhesion (IUA), premature ovarian insufficiency (POI), and polycystic ovary syndrome (PCOS), are great threats to female reproduction. Recently, mesenchymal stem cells derived-extracellular vesicles (MSC-EVs) have presented their potentials to cure these diseases, not only for the propensity ability they stemmed from the parent cells, but also for the higher biology stability and lower immunogenicity, compared to MSCs. EVs are lipid bilayer complexes, functional as mediators by transferring multiple molecules to recipient cells, such as proteins, microRNAs, lipids, and cytokines. Read More

View Article and Full-Text PDF

Gold(I) Complexes with a Quinazoline Carboxamide Alkynyl Ligand: Synthesis, Cytotoxicity, and Mechanistic Studies.

Eur J Inorg Chem 2021 May 11;2021(20):1921-1928. Epub 2021 May 11.

School of Chemistry National University of Ireland Galway Galway Ireland.

A series of gold(I) complexes with the general formula [Au()()] (=4-phenyl--(prop-2-yn-1-yl)quinazoline-2-carboxamide, =PPh (triphenylphosphine), ; TPA (1,3,5-triaza-7-phosphaadamantane), , and Me-imy (1,3-dimethylimidazol-2-ylidene), ) were synthesized and fully characterized by spectroscopic methods. The alkynyl ligand belongs to the quinazoline carboxamide class of ligands that are known to bind to the translocator protein (TSPO) at the outer mitochondrial membrane. and exert cytotoxic effects in bladder cancer cells with IC values in the low micromolar range. Read More

View Article and Full-Text PDF

A systematic review and meta-analysis of studies that have evaluated the role of mitochondrial function and iron metabolism in frailty.

Clin Transl Sci 2021 Jul 9. Epub 2021 Jul 9.

Department of Cardiovascular Sciences and National Institute for Health Research Leicester Biomedical Research Unit in Cardiovascular Medicine, University of Leicester, Leicester, UK.

Frailty is a condition of global impairment due to depletion of physiological reserves. However, the underlying biological mechanisms are poorly understood. The aims of the current study were to identify the differences in mitochondrial function and iron metabolism between frail and nonfrail populations, and to investigate the contribution of different methodological approaches to the results. Read More

View Article and Full-Text PDF