6,972 results match your criteria complement regulatory

Enabling Precision Medicine in Cancer Care Through a Molecular Data Warehouse: The Moffitt Experience.

JCO Clin Cancer Inform 2021 May;5:561-569

Department of Epidemiology, Moffitt Cancer Center, Tampa, FL.

Purpose: The use of genomics within cancer research and clinical oncology practice has become commonplace. Efforts such as The Cancer Genome Atlas have characterized the cancer genome and suggested a wealth of targets for implementing precision medicine strategies for patients with cancer. The data produced from research studies and clinical care have many potential secondary uses beyond their originally intended purpose. Read More

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Yu-Ping-Feng Formula Exerts Antilung Cancer Effects by Remodeling the Tumor Microenvironment through Regulating Myeloid-Derived Suppressor Cells.

Evid Based Complement Alternat Med 2021 20;2021:6624461. Epub 2021 Apr 20.

Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.

Yu-Ping-Feng (YPF) formula is a classical prescription used for enhancing the body's immunity function in traditional Chinese medicine (TCM). In clinical practice, the YPF formula has been reported to exhibit antilung cancer and immunomodulatory effect. However, the relationship between them remains unclear. Read More

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An intact complement system dampens cornea inflammation during acute primary HSV-1 infection.

Sci Rep 2021 May 13;11(1):10247. Epub 2021 May 13.

Departments of Ophthalmology, The University of Oklahoma Health Sciences Center (OUHSC), 608 Stanton L. Young Blvd., DMEI PA415, Oklahoma City, OK. 73104, USA.

Corneal transparency is an essential characteristic necessary for normal vision. In response to microbial infection, the integrity of the cornea can become compromised as a result of the inflammatory response and the ensuing tissue pathology including neovascularization (NV) and collagen lamellae destruction. We have previously found complement activation contributes to cornea pathology-specifically, denervation in response to HSV-1 infection. Read More

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Transcriptional Profiles of Genes Related to Stress and Immune Response in Rainbow Trout () Symptomatically or Asymptomatically Infected With .

Front Immunol 2021 21;12:639489. Epub 2021 Apr 21.

Key Laboratory of Mariculture (Ocean University of China), Ministry of Education (KLMME), Ocean University of China, Qingdao, China.

Rainbow trout () is one of the most common aquaculture fish species worldwide. Vibriosis disease outbreaks cause significant setbacks to aquaculture. The stress and immune responses are bidirectionally modulated in response to the health challenges. Read More

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Monitoring T Cells Responses Mounted by Therapeutic Cancer Vaccines.

Front Mol Biosci 2021 15;8:623475. Epub 2021 Apr 15.

Cancer Immunology and Immunotherapy Research Unit, Cancer Research Malaysia, Subang Jaya, Malaysia.

With the regulatory approval of Provenge and Talimogene laherparepvec (T-VEC) for the treatment of metastatic prostate cancer and advanced melanoma respectively, and other promising clinical trials outcomes, cancer vaccine is gaining prominence as a cancer therapeutic agent. Cancer vaccine works to induce T cell priming, expansion, and infiltration resulting in antigen-specific cytotoxicity. Such an approach that can drive cytotoxicity within the tumor could complement the success of checkpoint inhibitors as tumors shown to have high immune cell infiltration are those that would respond well to these antibodies. Read More

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The First WHO International Standard for Adalimumab: Dual Role in Bioactivity and Therapeutic Drug Monitoring.

Front Immunol 2021 15;12:636420. Epub 2021 Apr 15.

Analytical and Biological Sciences Division, National Institute for Biological Standards and Control, Medicines and Healthcare Products Regulatory Agency, Potters Bar, United Kingdom.

The expanded availability of adalimumab products continues to widen patient access and reduce costs with substantial benefit to healthcare systems. However, the long-term success of these medicines is highly dependent on maintaining consistency in quality, safety and efficacy while minimizing any risk of divergence during life-cycle management. In recognition of this need and demand from global manufacturers, the World Health Organization (WHO) Expert Committee on Biological standardization established the WHO 1 International standard (IS) for Adalimumab (coded 17/236) in October 2019 with a defined unitage ascribed to each of the individual bioactivities evaluated in the study namely, TNF-α binding, TNF-α neutralization, complement dependent cytotoxicity and antibody-dependent cellular cytotoxicity. Read More

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Characterization and distribution of medicine vendors in 2 states in Nigeria: implications for scaling health workforce and family planning services.

Hum Resour Health 2021 May 1;19(1):60. Epub 2021 May 1.

Society for Family Health, Abuja, Nigeria.

Background: In 2014, Nigeria issued the task-shifting/sharing policy for essential health services, which aimed to fill the human resource gap and improve the delivery of health services across the country. This study focuses on the characteristics, spread, and family planning (FP) stocking practices of medicine vendors in Lagos and Kaduna, assessing the influence of medical training on the provision and stocking of FP services and commodities by vendors.

Methods: We conducted a census of all Patent Medicines stores (PMS) followed up with a facility assessment among 10% of the mapped shops, utilizing an interviewer-administered questionnaire. Read More

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Sex-specific DNA methylation differences in Alzheimer's disease pathology.

Acta Neuropathol Commun 2021 Apr 26;9(1):77. Epub 2021 Apr 26.

Division of Biostatistics, Department of Public Health Sciences, University of Miami, Miller School of Medicine, Miami, FL, 33136, USA.

Sex is an important factor that contributes to the clinical and biological heterogeneities in Alzheimer's disease (AD), but the regulatory mechanisms underlying sex disparity in AD are still not well understood. DNA methylation is an important epigenetic modification that regulates gene transcription and is known to be involved in AD. We performed the first large-scale sex-specific meta-analysis of DNA methylation differences in AD neuropathology, by re-analyzing four recent epigenome-wide association studies totaling more than 1000 postmortem prefrontal cortex brain samples using a uniform analytical pipeline. Read More

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Evolution of the Subgroup 6 Genes and Their Contribution to Floral Color in the Perianth-Bearing Piperales.

Front Plant Sci 2021 9;12:633227. Epub 2021 Apr 9.

Facultad de Ciencias Exactas y Naturales, Instituto de Biología, Universidad de Antioquia, Medellín, Colombia.

Flavonoids, carotenoids, betalains, and chlorophylls are the plant pigments responsible for floral color. Anthocyanins, a class of flavonoids, are largely responsible for the red, purple, pink, and blue colors. genes belonging to subgroup 6 (SG6) are the upstream regulatory factors of the anthocyanin biosynthetic pathway. Read More

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Ex-TFRs: A Missing Piece of the SLE Puzzle?

Front Immunol 2021 9;12:662305. Epub 2021 Apr 9.

Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China.

Systemic lupus erythematosus (SLE) is a chronic multi-organ autoimmune disease involving the production of a wide range of autoantibodies and complement activation. The production of these high-affinity autoantibodies requires T cell/B cell collaboration as well as germinal center (GC) formation. T follicular regulatory cells (TFRs) are functional specialized T regulatory cells (Tregs) that safeguard against both self-reactive T and B cells. Read More

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Enhancing trust in AI through industry self-governance.

J Am Med Inform Assoc 2021 Apr 25. Epub 2021 Apr 25.

Departments of Biomedical Informatics, Biostatistics, and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Artificial intelligence (AI) is critical to harnessing value from exponentially growing health and healthcare data. Expectations are high for AI solutions to effectively address current health challenges. However, there have been prior periods of enthusiasm for AI followed by periods of disillusionment, reduced investments, and progress, known as "AI Winters. Read More

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Utilization of traditional medicine and its integration into the healthcare system in Qokolweni, South Africa; prospects for enhanced universal health coverage.

Complement Ther Clin Pract 2021 May 20;43:101386. Epub 2021 Apr 20.

University of Gothenburg, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy, Box 463, 405-30 Gothenburg, Sweden. Electronic address:

Despite the WHO providing a framework for the integration of traditional medicines into the health systems with pandemics like HIV/AIDS and the COVID-19, most countries are yet to adopt the recommendations. This study explored why the integration of traditional medicines with the biomedical healthcare system in Qokolweni in Eastern Cape, South Africa was stalling. The research employed qualitative research methods; participant observation and in-depth ethnographic interviews of community members, traditional healers, and nurses. Read More

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The persistent pain transcriptome: identification of cells and molecules activated by hyperalgesia.

J Pain 2021 Apr 20. Epub 2021 Apr 20.

National Institutes of Health Clinical Center, Department of Perioperative Medicine; Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia, Perelman School of Medicine at the University of Pennsylvania.

During persistent pain, the dorsal spinal cord responds to painful inputs from the site of injury, but the molecular modulatory processes have not been comprehensively examined. Using transcriptomics and multiplex in situ hybridization, we identified the most highly regulated receptors and signaling molecules in rat dorsal spinal cord in peripheral inflammatory and post-surgical incisional pain models. We examined a time course of the response including acute (2 hrs) and longer term (2 day) time points after peripheral injury representing the early onset and instantiation of hyperalgesic processes. Read More

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The thick aleurone1 Gene Encodes a NOT1 Subunit of the CCR4-NOT Complex and Regulates Cell Patterning in Endosperm.

Plant Physiol 2020 Oct;184(2):960-972

Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa 50011.

Maize (Zea mays) thick aleurone1 (thk1-R) mutants form multiple aleurone layers in the endosperm and have arrested embryogenesis. Prior studies suggest that thk1 functions downstream of defective kernel1 (dek1) in a regulatory pathway that controls aleurone cell fate and other endosperm traits. The original thk1-R mutant contained an ∼2-Mb multigene deletion, which precluded identification of the causal gene. Read More

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October 2020

Interferon-α activates interleukin-1 receptor-associated kinase 1 to induce regulatory T-cell apoptosis in patients with systemic lupus erythematosus.

J Dermatol 2021 Apr 21. Epub 2021 Apr 21.

Department of Dermatology, Dermatology Hospital, Southern Medical University, Guangzhou, China.

Impaired regulatory T-cell (Treg) responses and upregulated interleukin-1 receptor-associated kinase 1 (IRAK1) expression are associated with the development of human systemic lupus erythematosus (SLE). Here, we show that the levels of upregulated IRAK1 expression in circulating Tregs are correlated with the percentages of apoptotic Tregs, Systemic Lupus Erythematosus Disease Activity Index scores, and serum complement C3 levels in SLE patients. High levels of plasma interferon (IFN)-α in SLE patients induced IRAK1 phosphorylation to trigger Treg apoptosis, which was mitigated by IRAK1 inhibitor (IRAK-Inh) treatment. Read More

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Telestroke Across the Continuum of Care: Lessons from the COVID-19 Pandemic.

J Stroke Cerebrovasc Dis 2021 Apr 8;30(7):105802. Epub 2021 Apr 8.

Department of Neurology, University of Virginia, Charlottesville, VA, USA; Department of Public Health Sciences, University of Virginia, Charlottesville, VA, USA.

While use of telemedicine to guide emergent treatment of ischemic stroke is well established, the COVID-19 pandemic motivated the rapid expansion of care via telemedicine to provide consistent care while reducing patient and provider exposure and preserving personal protective equipment. Temporary changes in re-imbursement, inclusion of home office and patient home environments, and increased access to telehealth technologies by patients, health care staff and health care facilities were key to provide an environment for creative and consistent high-quality stroke care. The continuum of care via telestroke has broadened to include prehospital, inter-facility and intra-facility hospital-based services, stroke telerehabilitation, and ambulatory telestroke. Read More

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Investigating the Utility of Humanized PXR-CAR-CYP3A4/7 Mouse Model to Assess CYP3A-Mediated Induction.

Drug Metab Dispos 2021 Apr 16. Epub 2021 Apr 16.

Preclinical Development Sciences, ORIC Pharmaceuticals, United States

Clinical induction liability is assessed with human hepatocytes. However, underpredictions in the magnitude of clinical induction have been reported. Unfortunately, in vivo studies in animals do not provide additional insight due to species differences in drug metabolizing enzymes and their regulatory pathways. Read More

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Construction of liver hepatocellular carcinoma-specific lncRNA-miRNA-mRNA network based on bioinformatics analysis.

PLoS One 2021 16;16(4):e0249881. Epub 2021 Apr 16.

Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Liver hepatocellular carcinoma (LIHC) is one of the major causes of cancer-related death worldwide with increasing incidences, however there are very few studies about the underlying mechanisms and pathways in the development of LIHC. We obtained LIHC samples from The Cancer Genome Atlas (TCGA) to screen differentially expressed mRNAs, lncRNAs, miRNAs and driver mutations. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, Gene ontology enrichment analyses and protein-protein interaction (PPI) network were performed. Read More

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Mechanisms of enhancer action: the known and the unknown.

Genome Biol 2021 Apr 15;22(1):108. Epub 2021 Apr 15.

Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.

Differential gene expression mechanisms ensure cellular differentiation and plasticity to shape ontogenetic and phylogenetic diversity of cell types. A key regulator of differential gene expression programs are the enhancers, the gene-distal cis-regulatory sequences that govern spatiotemporal and quantitative expression dynamics of target genes. Enhancers are widely believed to physically contact the target promoters to effect transcriptional activation. Read More

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The dual role of complement in cancers, from destroying tumors to promoting tumor development.

Cytokine 2021 Jul 10;143:155522. Epub 2021 Apr 10.

Department of Medical Oncology, Hubei Cancer Hospital, the Seventh Clinical School Affiliated of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Colorectal Cancer Clinical Research Center of HuBei Province, Wuhan, China; Colorectal Cancer Clinical Research Center of Wuhan, Wuhan, China. Electronic address:

Complement is an important branch of innate immunity; however, its biological significance goes far beyond the scope of simple nonspecific defense and involves a variety of physiological functions, including the adaptive immune response. In this review, to unravel the complex relationship between complement and tumors, we reviewed the high diversity of complement components in cancer and the heterogeneity of their production and activation pathways. In the tumor microenvironment, complement plays a dual regulatory role in the occurrence and development of tumors, affecting the outcomes of the immune response. Read More

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The reduced ARF regulatory system in Giardia intestinalis pre-dates the transition to parasitism in the lineage Fornicata.

Int J Parasitol 2021 Apr 20. Epub 2021 Apr 20.

Division of Infectious Diseases, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada; Institute of Parasitology Biology Centre, CAS v.v.i. Branisovska 31, 370 05 Ceske Budejovice, Czech Republic. Electronic address:

Giardia intestinalis is an enteric pathogen with an extremely modified membrane trafficking system, lacking canonical compartments such as the Golgi, endosomes, and intermediate vesicle carriers. By comparison the fornicate relatives of Giardia possess greater endomembrane system complexity. In eukaryotes, the ADP ribosylation factor (ARF) GTPase regulatory system proteins, which consist of the small GTPase ARF1, and its guanine exchange nucleotide factors (GEFs) and GTPase activating proteins (GAPs), coordinate temporal and directional trafficking of cargo vesicles by recognizing and interacting with heterotetrameric coat complexes at pre-Golgi and post-Golgi interfaces. Read More

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Detection of Genetic Rearrangements in the Regulators of Complement Activation RCA Cluster by High-Throughput Sequencing and MLPA.

Methods Mol Biol 2021 ;2227:159-178

Centro de Investigaciones Biológicas and Ciber de Enfermedades Raras, Madrid, Spain.

The regulators of complement activation (RCA) gene cluster in 1q31-1q32 includes most of the genes encoding complement regulatory proteins. Genetic variability in the RCA gene cluster frequently involve copy number variations (CNVs), a type of chromosome structural variation causing alterations in the number of copies of specific regions of DNA. CNVs in the RCA gene cluster often relate with gene rearrangements that result in the generation of novel genes, carrying internal duplications or deletions, and hybrid genes, resulting from the fusion or exchange of genetic material between two different genes. Read More

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January 2021

Hemolytic Tests Exploring Factor H Functional Activities.

Methods Mol Biol 2021 ;2227:69-81

INSERM, UMRS 1138, "Inflammation, Complement and Cancer" Team, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, France.

Impairment of the complement regulatory protein Factor H (FH) is implicated in the physiopathological mechanisms of different diseases like atypical hemolytic and uremic syndrome and C3 glomerulopathies. It may be due to genetic abnormalities or acquired with the development of autoantibodies. FH has several ligands; therefore, the exploration of its functions requires to perform different tests. Read More

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January 2021

Treatment of Localized Vulvar Pain with Neural Therapy: A Case Series and Literature Review.

Complement Med Res 2021 Apr 12:1-7. Epub 2021 Apr 12.

Campus Docent de Sant Joan de Déu, Barcelona, Spain.

Background: Localized vulvar pain (LVP) is a common condition among fertile women, with physical and psychosexual implications. Treatment is complex with limited benefits. Neural therapy is a regulatory therapy that uses injections of local anesthetics in low concentrations in specific points to treat different conditions. Read More

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Effect of Xingbi Gel Nasal Drops on Fyn-STAT5 Pathway in Nasal Mucosa Fibroblasts of Guinea Pigs with Allergic Rhinitis.

Evid Based Complement Alternat Med 2021 20;2021:6686815. Epub 2021 Mar 20.

People's Hospital, Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China.

Fyn-STAT5 is considered to be the frontier signaling pathway of IgE-mediated allergic reactions related to mast cell activation, but research on allergic rhinitis (AR) has been rarely reported. Xingbi gel nasal drops (XGND) are a compound preparation of traditional Chinese medicine, which has the exact therapeutic efficacy on AR. The current study aimed to observe the effects of XGND on Fyn-STAT5 pathway in AR guinea pig nasal mucosal fibroblasts and further illuminate the possible therapeutic mechanism of XGND on AR. Read More

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Causative role of mast cell and mast cell-regulatory function of disialyllacto-N-tetraose in necrotizing enterocolitis.

Int Immunopharmacol 2021 Mar 31;96:107597. Epub 2021 Mar 31.

School of Pharmacy, School of Medicine, Changzhou University, Changzhou, Jiangsu 213164, China. Electronic address:

Necrotizing enterocolitis (NEC) remains a fatal gastrointestinal disorder in neonates. Disialyllacto-N-tetraose (DSLNT), a function-unclear human milk-derived hexasaccharide, shows anti-NEC potential in previous animal studies. This study is aimed to explore the role of mast cell (MC), a fundamental cell type of mucosal immune system and protective DSLNT in regulating pathological process of NEC. Read More

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Analysis of PI3K Pathway Associated Molecules Reveals Dysregulated Innate and Adaptive Functions of B Cells in Early Diffuse Cutaneous Systemic Sclerosis.

Int J Mol Sci 2021 Mar 12;22(6). Epub 2021 Mar 12.

Clinical Center, Department of Immunology and Biotechnology, University of Pécs Medical School, H-7624 Pécs, Hungary.

B cell activation is an early event in the development of systemic sclerosis (SSc). The classical activation of B cells downstream of the B-cell receptor (BCR) involves the phosphatidylinositol-3 kinase (PI3K) pathway that integrates the effects of multiple co-stimulatory receptors. Our analysis of PI3K pathway associated molecules in peripheral blood B cells of early diffuse cutaneous SSc (dcSSc) patients showed altered mRNA expression of Toll-like receptor (TLR) homolog CD180, TLR4, complement component 3, IL-4 receptor and secreted phosphoprotein 1 (SPP1). Read More

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Behavioral and Gene Expression Analysis of Stxbp6-Knockout Mice.

Brain Sci 2021 Mar 29;11(4). Epub 2021 Mar 29.

Center for Medical Genetics, School of Life Sciences, Central South University, Changsha 410008, China.

Since the first report that Stxbp6, a brain-enriched protein, regulates the assembly of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, little has been discovered about its functions over the past two decades. To determine the effects of Stxbp6 loss on nervous-system-associated phenotypes and underlying mechanisms, we constructed a global Stxbp6-knockout mouse. We found that Stxbp6-null mice survive normally, with normal behavior, but gained less weight relative to age- and sex-matched wildtype mice. Read More

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Complement Regulation in Human Tenocytes under the Influence of Anaphylatoxin C5a.

Int J Mol Sci 2021 Mar 18;22(6). Epub 2021 Mar 18.

Institute of Anatomy and Cell Biology, Paracelsus Medical University, Nuremberg and Salzburg, General Hospital Nuremberg, Prof. Ernst Nathan Str. 1, 90419 Nuremberg, Germany.

A central part of the complement system, the anaphylatoxin C5a was investigated in this study to learn its effects on tenocytes in respect to understanding the potential expression of other crucial complement factors and pro-inflammatory mediators involved in tendinopathy. Human hamstring tendon-derived tenocytes were treated with recombinant C5a protein in concentrations of 25 ng/mL and 100 ng/mL for 0.5 h (early phase), 4 h (intermediate phase), and 24 h (late phase). Read More

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