264,966 results match your criteria combination agents


Lysosomal alkalization to potentiate eradication of intraosteoblastic Staphylococcus aureus in the bone and joint infection setting.

Clin Microbiol Infect 2021 May 4. Epub 2021 May 4.

CIRI - Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Supérieure de Lyon, Univ Lyon, F-69007, Lyon, France; Université Claude Bernard Lyon 1, Lyon, France; Centre de Référence pour la prise en charge des Infections ostéo-articulaires complexes (CRIOAc Lyon; www.crioac-lyon.fr), Hospices Civils de Lyon, Lyon, France; Service des maladies infectieuses et tropicales, Hospices Civils de Lyon, Lyon, France. Electronic address:

Objectives: Beyond intracellular penetration, acidic lysosomal pH might affect the intracellular activity of some antimicrobials. This study evaluated the ability of lysosomotropic alkalizing agents to potentiate antimicrobial eradication of intraosteoblastic S. aureus reservoir in the bone and joint infection (BJI) setting. Read More

View Article and Full-Text PDF

Comparative study of the chemical composition, antibacterial activity and synergic effects of the essential oils of Croton tetradenius baill. And C. pulegiodorus baill. Against Staphylococcus aureus isolates.

Microb Pathog 2021 May 4:104934. Epub 2021 May 4.

Laboratory of Biofilms and Antimicrobial Agents (LaBAM), Federal University of Ceará, Sobral, 62048-280, Ceará, Brazil; Center of Molecular Bioprospecting and Applied Experimentation (NUBEM), University Center INTA - UNINTA, Sobral, 62050-100, Ceará, Brazil. Electronic address:

The aim of this work was to evaluate the chemical composition and antibacterial activity of Croton tetradenius Baill. (CTEO) and C. pulegiodorus Baill. Read More

View Article and Full-Text PDF

Cardiac safety of trabectedin monotherapy or in combination with pegylated liposomal doxorubicin in patients with sarcomas and ovarian cancer.

Cancer Med 2021 May 7. Epub 2021 May 7.

Sarcoma Center, Department of Medical Oncology, Dana-Farber Cancer Institute (DFCI), Harvard Medical School and Ludwig Center at Harvard, Boston, MA, USA.

Background: As with other alkylating agents, cardiac dysfunction can occur with trabectedin therapy for advanced soft tissue sarcomas (STS) or recurrent ovarian cancer (ROC) where treatment options for advanced disease are still limited. Cardiac safety for trabectedin monotherapy (T) for STS or in combination with pegylated liposomal doxorubicin (T+PLD) for ROC was evaluated in this retrospective postmarketing regulatory commitment.

Methods: Patient data for multiple cardiac-related treatment-emergent adverse events (cTEAEs) were evaluated in pooled analyses of ten phase 2 trials, one phase 3 trial in STS (n = 982), and two phase 3 trials in ROC (n = 1231). Read More

View Article and Full-Text PDF

Regional Gas Exchange Measured by Xe Magnetic Resonance Imaging Before and After Combination Bronchodilators Treatment in Chronic Obstructive Pulmonary Disease.

J Magn Reson Imaging 2021 May 7. Epub 2021 May 7.

Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA.

Background: Hyperpolarized Xe magnetic resonance imaging (MRI) provides a non-invasive assessment of regional pulmonary gas exchange function. This technique has demonstrated that chronic obstructive pulmonary disease (COPD) patients exhibit ventilation defects, reduced interstitial barrier tissue uptake, and poor transfer to capillary red blood cells (RBCs). However, the behavior of these measurements following therapeutic intervention is unknown. Read More

View Article and Full-Text PDF

PI3K signalling in chronic obstructive pulmonary disease and opportunities for therapy.

J Pathol 2021 May 7. Epub 2021 May 7.

Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Chronic obstructive pulmonary disease (COPD) is a chronic lung disease characterised by airway inflammation and progressive obstruction of the lung airflow. Current pharmacological treatments include bronchodilators, alone or in combination with steroids or other anti-inflammatory agents, which have only partially contributed to the inhibition of disease progression and mortality. Therefore, further research unravelling the underlying mechanisms is necessary to develop new anti-COPD drugs with both lower toxicity and higher efficacy. Read More

View Article and Full-Text PDF

Isoalantolactone Increases the Sensitivity of Prostate Cancer Cells to Cisplatin Treatment by Inducing Oxidative Stress.

Front Cell Dev Biol 2021 20;9:632779. Epub 2021 Apr 20.

Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Prostate cancer is the most common malignancy among men worldwide. Platinum (II)-based chemotherapy has been used to treat a number of malignancies including prostate cancer. However, the potential of cisplatin for treating prostate cancer is restricted owing to its limited efficacy and toxic side effects. Read More

View Article and Full-Text PDF

In silico and in vitro screening of licensed antimalarial drugs for repurposing as inhibitors of hepatitis E virus.

In Silico Pharmacol 2021 4;9(1):35. Epub 2021 May 4.

Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty ofScience, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.

Abstract: Hepatitis E virus (HEV) infection is emerging in Cameroon and represents one of the most common causes of acute hepatitis and jaundice. Moreover, earlier reports showed evidence of falciparum malaria/HEVcoexistence. Although the Sofosbuvir/Ribavirin combination was recently proposed in the treatment of HEV-infected patients, no specific antiviral drug has been approved so far, thereby urging the search for new therapies. Read More

View Article and Full-Text PDF

Bioaugmented Phytoremediation of Metal-Contaminated Soils and Sediments by Hemp and Giant Reed.

Front Microbiol 2021 20;12:645893. Epub 2021 Apr 20.

Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Piacenza, Italy.

We assessed the effects of EDTA and selected plant growth-promoting rhizobacteria (PGPR) on the phytoremediation of soils and sediments historically contaminated by Cr, Ni, and Cu. A total of 42 bacterial strains resistant to these heavy metals (HMs) were isolated and screened for PGP traits and metal bioaccumulation, and two spp. strains were finally selected. Read More

View Article and Full-Text PDF

The Ototoxicity of Antimalarial Drugs-A State of the Art Review.

Front Neurol 2021 20;12:661740. Epub 2021 Apr 20.

Balance Disorders Unit, Otolaryngology Department, The Norbert Barlicki Memorial Teaching Hospital, Medical University of Lodz, Lodz, Poland.

This review summarizes current knowledge about the occurrence of hearing and balance disorders after antimalarial drugs treatment. It also examines the clinical applications of antimalarials, their mechanisms behind this ototoxicity and how it can be monitored. It includes studies with larger numbers of patients and those in which auditory function was assessed using audiological tests. Read More

View Article and Full-Text PDF

In vitro Antimicrobial Synergy Testing of Extensively Drug-Resistant Clinical Isolates at an Organ Transplant Center in Nepal.

Infect Drug Resist 2021 30;14:1669-1677. Epub 2021 Apr 30.

School of Optometry and Vision Science, Faculty of Medicine and Health, UNSW, Sydney, NSW, Australia.

Purpose: Inappropriate use of broad-spectrum antibiotics contributes to the emergence of multidrug-resistant (MDR) bacteria. Finding novel antimicrobial agents and strategies based on synergistic combinations are essential to combat MDR infections. This study was designed to determine in vitro synergy of different antimicrobials against extensively drug-resistant (XDR) Gram-negative clinical isolates. Read More

View Article and Full-Text PDF

Targeting non-canonical activation of GLI1 by the SOX2-BRD4 transcriptional complex improves the efficacy of HEDGEHOG pathway inhibition in melanoma.

Oncogene 2021 May 6. Epub 2021 May 6.

Core Research Laboratory - Institute for Cancer Research and Prevention (ISPRO), Florence, Italy.

Despite the development of new targeted and immune therapies, the prognosis of metastatic melanoma remains bleak. Therefore, it is critical to better understand the mechanisms controlling advanced melanoma to develop more effective treatment regimens. Hedgehog/GLI (HH/GLI) signaling inhibitors targeting the central pathway transducer Smoothened (SMO) have shown to be clinical efficacious in skin cancer; however, several mechanisms of non-canonical HH/GLI pathway activation limit their efficacy. Read More

View Article and Full-Text PDF

Hypomethylating agents (HMA) for the treatment of acute myeloid leukemia and myelodysplastic syndromes: mechanisms of resistance and novel HMA-based therapies.

Leukemia 2021 May 6. Epub 2021 May 6.

Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Aberrant DNA methylation plays a pivotal role in tumor development and progression. DNA hypomethylating agents (HMA) constitute a class of drugs which are able to reverse DNA methylation, thereby triggering the re-programming of tumor cells. The first-generation HMA azacitidine and decitabine have now been in standard clinical use for some time, offering a valuable alternative to previous treatments in acute myeloid leukemia and myelodysplastic syndromes, so far particularly in older, medically non-fit patients. Read More

View Article and Full-Text PDF

Natural products targeting the synthesis of β(1,3)-D-glucan and chitin of the fungal cell wall. Existing drugs and recent findings.

Phytomedicine 2021 Mar 27:153556. Epub 2021 Mar 27.

Instituto de Biología Funcional y Genómica and Departamento de Microbiología y Genética, Consejo Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca, Salamanca, Spain. Electronic address:

Background: During the last three decades systemic fungal infections associated to immunosuppressive therapies have become a serious healthcare problem. Clinical development of new antifungals is an urgent requirement. Since fungal but not mammalian cells are encased in a carbohydrate-containing cell wall, which is required for the growth and viability of fungi, the inhibition of cell wall synthesizing machinery, such as β(1,3)-D-glucan synthases (GS) and chitin synthases (CS) that catalyze the synthesis of β(1-3)-D-glucan and chitin, respectively, represent an ideal mode of action of antifungal agents. Read More

View Article and Full-Text PDF

Current Therapy and Emerging Intravesical Agents to Treat Non-Muscle Invasive Bladder Cancer.

Hematol Oncol Clin North Am 2021 Jun 18;35(3):513-529. Epub 2021 Apr 18.

Department of Urology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA. Electronic address:

Transurethral resection of bladder tumor remains the cornerstone of non-muscle invasive bladder cancer management, proper risk stratification, and appropriate selection of adjuvant therapy. A single, postoperative dose of intravesical chemotherapy is used for low-risk patients; patients with high-grade, high-risk disease should receive intravesical bacillus Calmette-Guérin (BCG) induction and maintenance therapy. For patients who develop BCG-unresponsive disease, cystectomy remains the standard of care. Read More

View Article and Full-Text PDF

Targeted truncation of the ADAM17 cytoplasmic domain in mice results in protein destabilization and a hypomorphic phenotype.

J Biol Chem 2021 May 3:100733. Epub 2021 May 3.

Physiology, Biophysics and Systems Biology Program, Weill Cornell Medicine, New York, NY, 10021, USA; Arthritis and Tissue Degeneration Program, Hospital for Special Surgery, New York, NY, 10021, USA; Institute for Advanced Study, Technische Universität München, D-85748 Garching, Germany; Department of Medicine, Physiology and Systems Biology, Weill Cornell Medicine, New York, NY, 10021, USA; Department of Biophysics, Physiology and Systems Biology, Weill Cornell Medicine, New York, NY 10021, USA. Electronic address:

A disintegrin and metalloprotease 17 (ADAM17) is a cell-surface metalloprotease that serves as the principle sheddase for Tumor necrosis factor α (TNFα), interleukin-6 receptor (IL-6R) and several ligands of the epidermal growth factor receptor (EGFR), regulating these crucial signaling pathways. ADAM17 activation requires its transmembrane domain, but not its cytoplasmic domain, and little is known about the role of this domain in vivo. To investigate, we used CRISPR-Cas9 to mutate the endogenous Adam17 locus in mice to produce a mutant ADAM17 lacking its cytoplasmic domain (Adam17Δcyto). Read More

View Article and Full-Text PDF

Prescribing patterns for treating common complications of spinal cord injury.

J Spinal Cord Med 2021 May 6:1-9. Epub 2021 May 6.

Rural Clinical School in Family Medicine, University of New South Wales, Sydney, Australia.

Objective: The purpose of this study was to find the most and least commonly prescribed medications for treating secondary health complications associated with spinal cord injury (SCI); and determine overall polypharmacy rates and risk factors associated with it.

Design: Observational design, cross-sectional analysis.

Setting: Community; Canada. Read More

View Article and Full-Text PDF

Repurposing the FDA-approved anticancer agent ponatinib as a fluconazole potentiator by suppression of multidrug efflux and Pma1 expression in a broad spectrum of yeast species.

Microb Biotechnol 2021 May 6. Epub 2021 May 6.

State Key Laboratory of Oncogenes and Related Genes, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

Fungal infections have emerged as a major global threat to human health because of the increasing incidence and mortality rates every year. The emergence of drug resistance and limited arsenal of antifungal agents further aggravates the current situation resulting in a growing challenge in medical mycology. Here, we identified that ponatinib, an FDA-approved antitumour drug, significantly enhanced the activity of the azole fluconazole, the most widely used antifungal drug. Read More

View Article and Full-Text PDF

Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera).

J Arthropod Borne Dis 2020 Dec 31;14(4):416-424. Epub 2020 Dec 31.

Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Background: Flesh flies (Diptera: Sarcophagidae) are considered as myiasis agents and important evidences in forensic investigations. However, their use has been restricted because, at all larval stages and female adults, morphological species identification is difficult or very challenging. This study investigated to test utility of mitochondrial cytochrome oxidase subunit I (mt-COI) sequences for differentiation of six forensically important Iranian flesh flies namely, , , , , and . Read More

View Article and Full-Text PDF
December 2020

Association of Insulin Glargine Treatment with Bone Mineral Density in Patients with Type 2 Diabetes Mellitus.

Diabetes Metab Syndr Obes 2021 29;14:1909-1917. Epub 2021 Apr 29.

Department of Endocrinology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, People's Republic of China.

Purpose: To assess the association of type 2 diabetes mellitus (T2DM) and insulin glargine treatment with bone mineral density (BMD) in Chinese people.

Methods: This retrospective study included 50 subjects with T2DM: 25 received oral glucose-lowering medication (ORL group), and 25 received oral glucose-lowering medication in combination with insulin glargine injection (CGI group). Thirty non-diabetic control subjects were also included. Read More

View Article and Full-Text PDF

Synergistic Effect of Bazedoxifene and PARP Inhibitor in the Treatment of Ovarian Cancer Regardless of BRCA Mutation.

Anticancer Res 2021 May;41(5):2277-2286

Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, U.S.A.

Background/aim: Poly (ADP-ribose) polymerase inhibitors (PARPis) are one of the targeted therapies proven to treat breast cancer gene (BRCA)-mutant ovarian cancer. Because most ovarian cancers are BRCA wild-type, it is necessary to extend the usage of PARPis. In the present study, we combined the PARPi, talazoparib, and the IL-6 inhibitor, bazedoxifene, for the treatment of human ovarian cancer cells. Read More

View Article and Full-Text PDF

Topical Management of Peristomal Pyoderma Gangrenosum: A Report of 3 Case Studies.

J Wound Ostomy Continence Nurs 2021 May 3. Epub 2021 May 3.

Tomohiro Toyoda, MD, Department of Dermatology, Toho University Sakura Medical Center, Sakura, Japan. Shinji Mitsuyama, MD, PhD, Department of Dermatology, Toho University Sakura Medical Center, Sakura, Japan. Eri Nagao, MD, Department of Dermatology, Toho University Sakura Medical Center, Sakura, Japan. Fumihito Abe, MD, Department of Dermatology, Toho University Sakura Medical Center, Sakura, Japan. Masaaki Kimura, MD, Department of Dermatology, Toho University Sakura Medical Center, Sakura, Japan. Yurie Seido, RN, WOC Nurse, Department of Nursing Care, Toho University Sakura Medical Center, Sakura, Japan. Tetsuya Higuchi, MD, PhD, Department of Dermatology, Toho University Sakura Medical Center, Sakura, Japan.

Background: Peristomal pyoderma gangrenosum (PPG) presents multiple challenges for healthcare providers. The diagnosis of PPG may be delayed, and it may be mistaken for an irritant dermatitis or an infection. Patients with ostomies secondary to inflammatory bowel disease (IBD) may experience PPG. Read More

View Article and Full-Text PDF

Advances in Drug Discovery against Neglected Tropical Diseases: Human African and American Trypanosomiasis.

Curr Med Chem 2021 May 3. Epub 2021 May 3.

Medicinal Chemistry Research Laboratory, Department of Chemistry, Jamia Millia Islamia, Jamia Nagar, New Delhi-110025, India.

Human African and American trypanosomiasis are the vector-borne parasitic diseases that have killed millions of people early, and many people are still suffering from these neglected diseases. The causative agents of these infections are parasitic protozoans of the genus Trypanosoma. Current treatment regimens against these endemic diseases have several limitations in terms of safety, efficacy, route of administration, and some of them have lost efficacy due to the emergence of resistance in their respective parasites. Read More

View Article and Full-Text PDF

Mechanisms of Anti-Tumor Activity of (Ashwagandha).

Nutr Cancer 2021 17;73(6):914-926. Epub 2020 Jun 17.

Department of Human Genetics and Molecular Medicine, Central University of Punjab, Bathinda, Punjab, India.

Increasing herbal formulations have been used to treat several diseases including cancer. (Ashwagandha) is one such plant the extracts of which have been tested against a number of ailments including cancer, which remains as one of the most dreadful diseases on the globe. The ever-increasing number of cancer related mortality demands the development of novel chemopreventive agents with minimum side effects. Read More

View Article and Full-Text PDF

Development of novel apoptosis-assisted lung tissue decellularization methods.

Biomater Sci 2021 May;9(9):3485-3498

J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.

Decellularized tissues hold great potential for both regenerative medicine and disease modeling applications. The acellular extracellular matrix (ECM)-enriched scaffolds can be recellularized with patient-derived cells prior to transplantation, or digested to create thermally-gelling ECM hydrogels for 3D cell culture. Current methods of decellularization clear cellular components using detergents, which can result in loss of ECM proteins and tissue architectural integrity. Read More

View Article and Full-Text PDF

Injectable hydrogel-mediated combination of hyperthermia ablation and photo-enhanced chemotherapy in the NIR-II window for tumor eradication.

Biomater Sci 2021 May;9(9):3516-3525

School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, China.

Local administration of therapeutic agents with long-term retention capabilities efficiently avoids nonspecific distribution in normal organs with an increased drug concentration in pathological tissue. Herein, we developed an injectable and degradable alginate-calcium (Ca2+) hydrogel for the local administration of corn-like Au/Ag nanorods (NRs) and doxorubicin hydrochloride (DOX·HCl). The immobilized Au/Ag NRs with strong absorbance in the near-infrared II (NIR-II) window efficiently ablated the majority of tumor cells after 1064 nm laser irradiation and triggered the release of DOX to kill residual tumor cells. Read More

View Article and Full-Text PDF

Natural History of Afferent Baroreflex Failure in Adults.

Neurology 2021 May 4. Epub 2021 May 4.

Department of Neurology, Mayo Clinic, Rochester, MN

Objective: To describe the natural history of afferent baroreflex failure (ABF) based on systematic review of clinical and laboratory data in patients with a diagnosis of ABF at Mayo Clinic Rochester.

Methods: We performed a retrospective chart review of all patients who underwent standardized autonomic reflex testing between 2000 and 2020 and had confirmation of the diagnosis of ABF by an autonomic disorders specialist. Patients were identified using a data repository of medical records. Read More

View Article and Full-Text PDF

The Potency of Seaweed Sulfated Polysaccharides for the Correction of Hemostasis Disorders in COVID-19.

Molecules 2021 Apr 29;26(9). Epub 2021 Apr 29.

G.P. Somov Institute of Epidemiology and Microbiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, 690087 Vladivostok, Russia.

Hemostasis disorders play an important role in the pathogenesis, clinical manifestations, and outcome of COVID-19. First of all, the hemostasis system suffers due to a complicated and severe course of COVID-19. A significant number of COVID-19 patients develop signs of hypercoagulability, thrombocytopenia, and hyperfibrinolysis. Read More

View Article and Full-Text PDF

In Vitro Inhibitory Analysis of Rationally Designed siRNAs against MERS-CoV Replication in Huh7 Cells.

Molecules 2021 Apr 29;26(9). Epub 2021 Apr 29.

Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

MERS-CoV was identified for the first time in Jeddah, Saudi Arabia in 2012 in a hospitalized patient. This virus subsequently spread to 27 countries with a total of 939 deaths and 2586 confirmed cases and now has become a serious concern globally. Camels are well known for the transmission of the virus to the human population. Read More

View Article and Full-Text PDF

Immune Checkpoint Inhibitors: A Promising Treatment Option for Metastatic Castration-Resistant Prostate Cancer?

Int J Mol Sci 2021 Apr 29;22(9). Epub 2021 Apr 29.

Badalona Applied Research Group in Oncology (B·ARGO), Catalan Institute of Oncology, Ctra. Can Ruti-Camí de les Escoles s/n, 08916 Badalona, Spain.

Since 2010, several treatment options have been available for men with metastatic castration-resistant prostate cancer (mCRPC), including immunotherapeutic agents, although the clinical benefit of these agents remains inconclusive in unselected mCRPC patients. In recent years, however, immunotherapy has re-emerged as a promising therapeutic option to stimulate antitumor immunity, particularly with the use of immune checkpoint inhibitors (ICIs), such as PD-1/PD-L1 and CTLA-4 inhibitors. There is increasing evidence that ICIs may be especially beneficial in specific subgroups of patients with high PD-L1 tumor expression, high tumor mutational burden, or tumors with high microsatellite instability/mismatch repair deficiency. Read More

View Article and Full-Text PDF