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"Biomethanation of syngas by enriched mixed anaerobic consortium in pressurized agitated column".

Bioresour Technol 2021 Jul 14;338:125548. Epub 2021 Jul 14.

Univ Lyon, INSA Lyon, DEEP, EA7429, 69621 Villeurbanne, France. Electronic address:

In a circular economy approach, heterogeneous wastes can be upgraded to energy in the form of syngas via pyrogasification, and then to methane via biomethanation. Working at high pressure is a promising approach to intensify the process and to reduce gas-liquid transfer limitations. However, raising the pressure could lead to reaching the CO inhibition threshold of the microorganisms involved in syngas-biomethanation. Read More

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Plausible Emergence and Self Assembly of a Primitive Phospholipid from Reduced Phosphorus on the Primordial Earth.

Orig Life Evol Biosph 2021 Jul 19. Epub 2021 Jul 19.

Instituto de Astrobiología de Colombia, Bogotá, Colombia.

How life arose on the primitive Earth is one of the biggest questions in science. Biomolecular emergence scenarios have proliferated in the literature but accounting for the ubiquity of oxidized (+ 5) phosphate (PO) in extant biochemistries has been challenging due to the dearth of phosphate and molecular oxygen on the primordial Earth. A compelling body of work suggests that exogenous schreibersite ((Fe,Ni)P) was delivered to Earth via meteorite impacts during the Heavy Bombardment (ca. Read More

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A method for the expression of fibroblast growth factor 14 and assessment of its neuroprotective effect in an Alzheimer's disease model.

Ann Transl Med 2021 Jun;9(12):994

Department of Dermatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background: Fibroblast growth factor (FGF) 14 is a member of the FGF family that is mainly expressed in the central nervous system. FGF14 has a close association with the occurrence of neurodegenerative conditions; however, its significance in Alzheimer's disease (AD) has yet to be evaluated. Therefore, we sought to obtain a large amount of exogenous FGF14 protein and explore its effect in a cellular model of AD. Read More

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Determination of bisphosphonates anti-resorptive properties based on three forms of ceramic materials: Sorption and release process evaluation.

J Pharm Anal 2021 Jun 15;11(3):364-373. Epub 2020 Aug 15.

Wrocław University of Science and Technology, Faculty of Chemistry, Department of Bioorganic Chemistry, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.

There is a strong need to search for more effective compounds with bone anti-resorptive properties, which will cause fewer complications than commonly used bisphosphonates. To achieve this goal, it is necessary to search for new techniques to characterize the interactions between bone and drug. By studying their interaction with hydroxyapatite (HA), this study used three forms of ceramic materials, two of which are bone-stimulating materials, to assess the suitability of new active substances with anti-resorptive properties. Read More

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A simple and rapid method to simultaneously analyze ciclesonide and its impurities in a ciclesonide metered-dose inhaler using on-line supercritical fluid extraction/supercritical fluid chromatography/quadrupole time-of-flight mass spectrometry.

J Pharm Biomed Anal 2021 Jul 9;204:114253. Epub 2021 Jul 9.

National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 210-9501, Japan.

A simple and rapid on-line SFE/SFC/quadrupole TOF-MS method to simultaneously analyze active pharmaceutical ingredients and impurities from metered-dose inhalers (MDIs) was developed using ciclesonide MDI (CIC-MDI) as an example. CIC-MDI, as drug Alvesco®, has been approved for the treatment of bronchial asthma, and its major impurities are listed in the European Pharmacopoeia and in the supplementary package inserts of Alvesco® (called as "Pharmaceutical interview form" in Japan). In the developed method, CIC-MDI was manually sprayed only once on a glass disc prior to the SFE/SFC/quadrupole TOF-MS. Read More

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Osteological development of a small and fast metamorphic frog, Microhyla fissipes (Anura, Neobatrachia, Microhylidae).

J Anat 2021 Jul 15. Epub 2021 Jul 15.

CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

Describing osteological development is of great importance for understanding vertebrate phenotypic variations, form-functional transitions and ecological adaptations. Anurans exhibit dramatic changes in their morphology, habitat preferences, diet and behaviour between the tadpole and frog stages. However, the anatomical details of their cranial and postcranial development have not been extensively studied, especially in Microhylidae. Read More

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A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker.

Int J Anal Chem 2021 26;2021:5533646. Epub 2021 Jun 26.

Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, China.

In this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists-ondansetron, azasetron, ramosetron, granisetron, and tropisetron-either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. The quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. The quantitative analysis of the 5-HT receptor antagonists was calculated and investigated based on the relative correction factors. Read More

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Does silica addition affect translocation and leaching of cadmium and copper in soil?

Environ Pollut 2021 Jul 9;288:117738. Epub 2021 Jul 9.

Fachgebiet Bodenchemie mit Pedologie, Institut für Bodenkunde und Standortslehre, Universität Hohenheim, 70593, Stuttgart, Germany. Electronic address:

Soil and groundwater contamination with potentially toxic elements (PTEs) including cadmium (Cd) and copper (Cu) has become a serious problem for ecosystem functioning. Silicon (Si) may precipitate these metals as silicates, and may also form, at undersaturation of silicates, 'Si-contaminant compounds', i.e. Read More

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Extensive cervicothoracic posterior arch defects from C1 to T6 leading to myelopathy due to thoracic kyphosis in an adolescent boy.

Spine Deform 2021 Jul 12. Epub 2021 Jul 12.

Department of Orthopaedics, PD Hinduja Hospital and Medical Research Center, Mumbai, Maharashtra, India.

Purpose: Limited dorsal myeloschisis, a form of cervical spinal dysraphism, is a rare anomaly and is typically associated with spinal cord tethering. The objective is to illustrate a rare dysraphic anomaly in the cervicothoracic spine causing myelopathy, not due to tethering but secondary to progressive kyphosis. To our knowledge, such an anomaly has not been described in the literature. Read More

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Spatial representation of in-stream sediment phosphorus release combining channel network approaches and in-situ experiments.

Sci Total Environ 2021 Jun 29;795:148790. Epub 2021 Jun 29.

The James Hutton Institute, Craigiebuckler, Aberdeen AB158QH, UK.

Impairment of rivers by elevated phosphorus (P) concentration is an issue often studied at outlets of mesoscale catchments. Our objective was to evaluate within-catchment spatio-temporal processes along connected reaches to understand processes of internal P loading associated with sediment input, accumulations in channels and sediment-water column P exchange. Our overall hypothesis was that heterogeneous sediment residence within the channel of a 52 km mixed land cover catchment resulted in key zones for sediment-water P exchange. Read More

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Algal migration and nutrient enrichment contribute to patterns in phytoplankton versus epiphyton communities.

Sci Total Environ 2021 Jul 1;795:148747. Epub 2021 Jul 1.

Guangdong Provincial Key Laboratory of Water Quality Improvement and Ecological Restoration for Watersheds, Institute of Environmental and Ecological Engineering, Guangdong University of Technology, Guangzhou 510006, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China. Electronic address:

Algal dominance between phytoplankton and epiphyton plays an essential role in predicting shallow lake shifts between clear-water and turbid-water states. However, compared to resources competition, studies on algal life-form shifts between phytoplankton and epiphyton have traditionally received less interest, as few studies have focused on algal communities in both habitats concurrently. We conducted a 4 × 3 factorial design microcosm experiment to explore the mutual feedback relationship between phytoplankton and epiphyton. Read More

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Synthesis and HPLC enantioseparation of novel derivatives of 3-alkoxy-4-hydroxyphenylalkanones of a potential α/β-blocker type.

Ceska Slov Farm 2021 ;70(2):51-58

The present paper reports the synthesis of a series of seven compounds with a hetero aminopropanol chain. The compounds were prepared by the conversion of 3-alkoxy-4-hydroxyphenyl alkanones with 2-chloromethyl oxirane and subsequent reaction of the products with heterocyclic amines (pyrrolidine, azepane, 4-methylpiperazine and 2-methoxyphenyl piperazine). The target compounds were synthesized in the form of racemates. Read More

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A model of algal growth depending on nutrients and inorganic carbon in a poorly mixed water column.

J Math Biol 2021 Jul 8;83(2):15. Epub 2021 Jul 8.

School of Science, Harbin University of Science and Technology, Harbin, Heilongjiang, 150080, People's Republic of China.

A reaction-diffusion-advection model is proposed to describe the growth of algae depending on both nutrients and inorganic carbon in a poorly mixed water column. Nutrients from the water bottom and inorganic carbon from the water surface form an asymmetric resource supply mechanism for the algal growth. The existence and stability of semi-trivial steady state and positive steady state of the model are proved, and a threshold condition for the regime shift from extinction to survival of algae is established. Read More

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[Research progress on analytical methods for the determination of hexachlorobutadiene].

Se Pu 2021 Jan;39(1):46-56

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Hexachlorobutadiene (HCBD) is one of persistent organic pollutants (POPs) listed in Annex A and Annex C of the Stockholm Convention in 2015 and 2017, respectively. Research on the sources, environmental occurrences, and biological effects of HCBD has a great significance in controlling this newly added POPs. Sensitive and credible methods for the determination of HCBD are preconditions and form the basis for related research work. Read More

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January 2021

[Quantitative analysis of tryptophan and its metabolites in urine by ultra performance liquid chromatography-tandem mass spectrometry].

Se Pu 2021 May;39(5):518-525

Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.

Tryptophan (Trp), also known as α-amino -indolepropionic acid, is an essential amino acid, which is involved in various physiological processes. Studies have shown that tumors, infectious diseases, and neurological diseases are accompanied by Trp-related metabolic disorders. Understanding the excretion of Trp and its metabolites in normal individuals is of great significance for treating Trp-related diseases and monitoring the health. Read More

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[Determination of tetracycline and fluoroquinolone residues in fish by polydopamine nanofiber mat based solid phase extraction combined with ultra performance liquid chromatography-tandem mass spectrometry].

Se Pu 2021 Jun;39(6):624-632

Suzhou Center for Disease Control and Prevention, Suzhou 215100, China.

Tetracyclines and fluoroquinolones are common antibacterial drugs used in aquaculture, and their residues may pose a risk to human health. The low concentration of drug residues and complex matrixes such as fats and proteins in aquatic products necessitate the urgent development of efficient sample pretreatment methods. Solid phase extraction (SPE) is the most common sample pretreatment method, in which the core is an adsorbent. Read More

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Accelerated Stability Study of the Ester Prodrug Remdesivir; Recently FDA Approved Covid-19 Antiviral Using RP-HPLC with Fluorimetric and Diode Array Detection.

Biomed Chromatogr 2021 Jul 5:e5212. Epub 2021 Jul 5.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Damanhour University, Beheira, Egypt.

Remdesivir is the first antiviral drug, approved by the Food and Drug Administration, to treat SARS-CoV-2. Remdesivir is a relatively new chemical entity, "ester prodrug", with no reported stability profile. Due to the urge of its use and thus, fast production, it is important to develop a stability-indicating method for its assay. Read More

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Correlation between Length of Ala and Transverse Diameter of First Sacral Vertebral Body in both Sexes.

Mymensingh Med J 2021 Jul;30(3):589-593

Dr Nishat Sultana, Department of Anatomy, Mymensingh Medical College (MMC), Mymensingh, Bangladesh; E-mail:

Sacrum is a triangular bone formed by the fusion of five sacral vertebrae. It is situated near the lower end of the spinal column, where it joins both hip bones to form the posterio superior wall of the pelvic cavity. It is an important bone for identification of sex in human skeletal system. Read More

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[Determination of dacarbazine in the urine of mice with melanoma by high performance liquid chromatography].

Se Pu 2020 Nov;38(11):1302-1307

College of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.

Dacarbazine (DTIC) is a first-line chemotherapy drug that is widely used in clinical practice for malignant melanoma. DTIC is metabolized by the liver . Some drugs are excreted in urine in the form of a prototype. Read More

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November 2020

A Radionuclide Generator of High-Purity Bi-213 for Instant Labeling.

Pharmaceutics 2021 Jun 21;13(6). Epub 2021 Jun 21.

Institute for Nuclear Research of Russian Academy of Sciences, 60-Letiya Oktyabrya Prospekt 7a, 117312 Moscow-Troitsk, Russia.

A new two-column Ac/Bi generator was developed specifically for using Ac containing an impurity of long lived Ac. The parent Ac was retained on the first Actinide Resin column, while Bi was accumulated on the second column filled with AG MP-50 resin via continuous elution and decay of intermediate Fr. The Bi accumulation was realized in circulation mode which allowed a compact generator design. Read More

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A Molecular Shape Recognitive HPLC Stationary Phase Based on a Highly Ordered Amphiphilic Glutamide Molecular Gel.

Nanomaterials (Basel) 2021 Jun 15;11(6). Epub 2021 Jun 15.

Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.

Chiral glutamide-derived lipids form self-assembled fibrous molecular gels that can be used as HPLC organic phases. In this study, HPLC separation efficiency was improved through the addition of branched amphiphilic glutamide lipids to the side chains of a terminally immobilized flexible polymer backbone. Poly(4-vinylpyridine) with a trimethoxysilyl group at one end was grafted onto the surface of porous silica particles (Sil-VP, polymerization degree = 15), and the pyridyl side chains were quaternized with a glutamide lipid having a bromide group (Br). Read More

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Isocratic reporter-exclusion immunoassay using restricted-access adsorbents.

Analyst 2021 Jul 2. Epub 2021 Jul 2.

Department of Biology and Biochemistry, University of Houston, Houston, TX, USA. and Department of Chemical and Biomolecular Engineering, University of Houston, TX, USA and Department of Biomedical Engineering, University of Houston, Houston, TX, USA and Escuela de Medicina y Ciencias de las Salud ITESM, Monterrey, Mexico.

We introduce analyte-dependent exclusion of reporter reagents from restricted-access adsorbents as the basis of an isocratic reporter-exclusion immunoassay for viruses, proteins, and other analytes. Capto™ Core 700 and related resins possess a noninteracting size-selective outer layer surrounding a high-capacity nonspecific mixed-mode capture adsorbent core. In the absence of analyte, antibody-enzyme reporter conjugates can enter the adsorbent and be captured, and their signal is lost. Read More

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Economical irregular silica as an effective dispersive solid-phase extraction sorbent for the quantification of calcitriol in soft capsules.

J Pharm Biomed Anal 2021 Sep 24;203:114227. Epub 2021 Jun 24.

Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Pharmacy, China Pharmaceutical University, No.24, Tongjiaxiang, Nanjing, 210009, China; Department of Pharmaceutical Analysis, China Pharmaceutical University, No. 24, Tongjiaxiang, Nanjing, 210009, China. Electronic address:

Calcitriol is an active product of vitamin D produced by the liver and kidney hydroxylase metabolism with strong physiological activity. It is the preferred form of medicine for patients with insufficient bone mineralization due to chronic kidney disease. Calcitriol soft capsule is one of the common preparation forms, the main drug content of which is very low (1. Read More

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September 2021

A column-based deep learning method for the detection and quantification of atrophy associated with AMD in OCT scans.

Med Image Anal 2021 Jun 12;72:102130. Epub 2021 Jun 12.

School of Computer Science and Engineering, The Hebrew University of Jerusalem, Israel. Electronic address:

The objective quantification of retinal atrophy associated with age-related macular degeneration (AMD) is required for clinical diagnosis, follow-up, treatment efficacy evaluation, and clinical research. Spectral Domain Optical Coherence Tomography (OCT) has become an essential imaging technology to evaluate the macula. This paper describes a novel automatic method for the identification and quantification of atrophy associated with AMD in OCT scans and its visualization in the corresponding infrared imaging (IR) image. Read More

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Thoracolumbar Fracture in Disseminated Idiopathic Skeletal Hyperostosis.

Cureus 2021 May 25;13(5):e15222. Epub 2021 May 25.

Department of Orthopedics and Traumatology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Hospital Canselor Tuanku Muhriz, Kuala Lumpur, MYS.

Disseminated idiopathic skeletal hyperostosis (DISH) is a form of ankylosing spinal disorders, which is at high risk of fracture because of the rigidity of the spinal column and reduced bone quality. The patients with DISH are at higher risk of fall because of the poor muscle tone, rigid spine column, and positive sagittal balance. The management of spinal fractures in these patients proves to be challenging because of the altered biomechanics and alignment of the spine. Read More

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Mini-invasive bilateral transfacet screw fixation with reconstruction of the neural arch for lumbar stenosis: A two centre case series.

Surgeon 2021 Jun 26. Epub 2021 Jun 26.

Department of Musculoskeletal Disorders, Faculty of Medicine and Surgery, University of Salerno, 84084 Baronissi, Italy; Clinica Ortopedica, San Giovanni di Dio e Ruggi D'Aragona Hospital, 84131 Salerno, Italy; Queen Mary University of London, Barts and the London School of Medicine and Dentistry, Centre for Sports and Exercise Medicine, Mile End Hospital, 275 Bancroft Road, London, E1 4DG, England; School of Pharmacy and Bioengineering, Keele University Faculty of Medicine, Guy Hilton Research Centre, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, England. Electronic address:

Background And Purpose: Lumbar stenosis and instability frequently coexist. Spinal canal decompression is often combined with fixation of the relevant vertebral segment and can be performed using different techniques and devices, including pedicle screws and interspinous devices and facet screws. The present study evaluates the clinical outcome of laminectomy and single-level fusion using a minimally invasive technique for rigid posterior spinal column fixation with two cross-linked lag screws. Read More

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Seawater stratification vs. plankton for oligotrophic mechanism: A case study of M4 seamount area in the Western Pacific Ocean.

Mar Environ Res 2021 Jul 22;169:105400. Epub 2021 Jun 22.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; University of Chinese Academy of Sciences, Beijing, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China.

Oligotrophic sea area mainly distributes in tropical and subtropical ocean, which have a profound impact on the marine material cycle and the structure of biological community. Based on the comprehensive survey in M4 seamount area of the Tropical Western Pacific Ocean in August 2017, the nutrients characteristics were explained, the formation mechanism of oligotrophic characteristics in this region was explored, and the influence of M4 seamount on oligotrophic sea area was analyzed. The results showed that the M4 seamount area is a typical oligotrophic sea area. Read More

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Applicability of ferric(III) hydroxide as a phosphate-selective adsorbent for sewage treatment.

Water Sci Technol 2021 Jun;83(12):2911-2920

Institute of Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea E-mail:

This research was undertaken to evaluate the usability of ferric(III) hydroxide for phosphate removal from sewage. Batch adsorption experiments, partly fixed bed column experiments, were conducted to study the influence of various factors, competing anions and contact time on the adsorption of phosphate on ferric(III) hydroxide. Processing ferric iron in the form of akaganeite (β-FeOOH) greatly increased the adsorption capacity for phosphate. Read More

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Conformation-specific monoclonal antibodies recognizing the native structure of G protein-coupled receptor (GPCR).

Int Immunopharmacol 2021 Jun 25;98:107872. Epub 2021 Jun 25.

Division of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, Japan. Electronic address:

It is quite difficult to generate monoclonal antibodies that recognize the three-dimensional structures of the antigens of interest. To address this limitation, we developed a new hybridoma technology termed "optimized stereospecific targeting (SST)". Here we aimed at generating stereospecific monoclonal antibodies against a G protein-coupled receptor (GPCR). Read More

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Ann Pharm Fr 2021 Jun 19. Epub 2021 Jun 19.

Department of Pharmaceutical Analysis, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, 530045, Andhra Pradesh, India. Electronic address:

Objective: The objective of the present study is to develop and validate a simple, rapid stability indicating high performance liquid chromatography method for the simultaneous quantification of metformin, empagliflozin, linagliptin in bulk and pharmaceutical dosage form.

Materials & Methods: The chromatographic separation was achieved on C18 X-bridge phenyl column (250×4.6mm, 5µm particle size). Read More

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