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Pilot study on the decontamination efficacy of an installed 222-nm ultraviolet disinfection device (Care222™), with a motion sensor, in a shared bathroom.

Photodiagnosis Photodyn Ther 2021 May 7:102334. Epub 2021 May 7.

Department of Infectious Diseases, Hiroshima University Hospital, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan.

Background: Toilet surfaces are contaminated with pathogens, and they may be a vector for disease transmission. In this study, we investigated the efficacy of an automated 222-nm ultraviolet C (UVC) disinfection device "Care222™," with a motion sensor, for removing bacterial contamination in a shared bathroom.

Methods: Two automated UVC devices, deactivated by motion sensors, were mounted on the ceiling of two bathrooms; the emission window of the UVC device was covered in the non-treated bathroom. Read More

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Development of Donor Recipient Chimeric Cells of bone marrow origin as a novel approach for tolerance induction in transplantation.

Stem Cell Investig 2021 19;8. Epub 2021 Apr 19.

Department of Orthopaedics, University of Illinois at Chicago, Chicago, IL, USA.

Background: Cell therapies and chimerism-based strategies are currently the most successful approach for tolerance induction in transplantation. This study aimed to establish and characterize novel Donor Recipient Chimeric Ccell (DRCC) therapy of bone marrow (BM) origin presenting donor-recipient phenotype to support tolerance induction.

Methods: fusions of fully MHC-mismatched BM cells from ACI (RT1) and Lewis (RT1) rats were performed using polyethylene-glycol (PEG). Read More

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miRNA-193a-3p Regulates the AKT2 Pathway to Inhibit the Growth and Promote the Apoptosis of Glioma Cells by Targeting ALKBH5.

Front Oncol 2021 23;11:600451. Epub 2021 Apr 23.

Department of Neurosurgery, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Emerging evidence indicates that microRNA (miR)-193a-3p is involved in the tumor progression of various cancers. However, the biological functions and precise molecular mechanisms of miR-193a-3p in gliomas have not been well documented. Accordingly, this study focused on the tumor suppressor role and molecular mechanisms of miR-193a-3p in glioma cells. Read More

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Adrenergic Receptor Stimulation Promotes Reperfusion in Ischemic Limbs in a Murine Diabetic Model.

Front Pharmacol 2021 22;12:666334. Epub 2021 Apr 22.

University of Sydney, Faculty of Medicine and Health, Sydney, NSW, Australia.

Peripheral arterial disease (PAD) is a major burden, resulting in limb claudication, repeated surgical interventions and amputation. There is an unmet need for improved medical management of PAD that improves quality of life, maintains activities of daily life and reduces complications. Nitric oxide (NO)/redox balance is a key regulator of angiogenesis. Read More

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"Face mask contamination during COVID-19 pandemia. Study on patients receiving intravitreal injections".

Retina 2021 Apr 29. Epub 2021 Apr 29.

Department of Ophthalmology, University Hospital Virgen de la Victoria Málaga, Spain. Department of Microbiology, University Hospital Virgen de la Victoria Málaga, Spain. Universidad Internacional de la Rioja (UNIR), Spain.

Purpose: To investigate the bacterial growth in the surgical face masks used by patients who received intravitreal injections and study the effect of povidone-iodine on the periocular area (PA) of masks.

Methods: Forty patients who attended for intravitreal injections were divided in those with less (<4h) and more (>4h) than 4 hours of mask use. Each group was divided depending on the application or not of povidone-iodine in the periocular area (PA) of the mask. Read More

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Recombinant Lactobacillus plantarum NC8 strain expressing porcine rotavirus VP7 induces specific antibodies in BALB/c mice.

Acta Biochim Biophys Sin (Shanghai) 2021 May 8. Epub 2021 May 8.

College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

The major etiologic agent that causes acute gastroenteritis worldwide in young animals and children is Group A rotavirus. Currently, commercially available vaccines do not often prevent porcine rotavirus (PRV) infection. In this study, we evaluated the efficacy of oral recombinant Lactobacillus vaccine against PRV in a mouse model. Read More

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Sox9 and Rbpj differentially regulate endothelial to mesenchymal transition and wound scarring in murine endovascular progenitors.

Nat Commun 2021 May 7;12(1):2564. Epub 2021 May 7.

The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD, Australia.

Endothelial to mesenchymal transition (EndMT) is a leading cause of fibrosis and disease, however its mechanism has yet to be elucidated. The endothelium possesses a profound regenerative capacity to adapt and reorganize that is attributed to a population of vessel-resident endovascular progenitors (EVP) governing an endothelial hierarchy. Here, using fate analysis, we show that two transcription factors SOX9 and RBPJ specifically affect the murine EVP numbers and regulate lineage specification. Read More

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Preliminary study on impacts of polystyrene microplastics on the hematological system and gene expression in bone marrow cells of mice.

Ecotoxicol Environ Saf 2021 May 4;218:112296. Epub 2021 May 4.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China. Electronic address:

Microplastics (MPs) are currently a global environmental pollutants and health hazards that caused by MPs cannot be ignored. However, studies on MP toxicity in mammals are scare. Here, we investigated the effects of two doses (0. Read More

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Quercetin inhibits invasion and angiogenesis of esophageal cancer cells.

Pathol Res Pract 2021 Apr 28;222:153455. Epub 2021 Apr 28.

Department of Gastroenterology, Renmin Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China.

Background: Esophageal carcinoma has poor prognosis and novel therapies for esophageal carcinoma are urgently needed. Quercetin is a natural flavonoid compound that can be found in many foods. In this study, we investigated the effects of quercetin on invasion and angiogenesis of esophageal cancer cells. Read More

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Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures.

Stem Cell Reports 2021 Apr 27. Epub 2021 Apr 27.

Wellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK; York Biomedical Research Institute, Department of Biology, University of York, York YO10 5DD, UK. Electronic address:

Advances in the isolation and gene expression profiling of single hematopoietic stem cells (HSCs) have permitted in-depth resolution of their molecular program. However, long-term HSCs can only be isolated to near purity from adult mouse bone marrow, thereby precluding studies of their molecular program in different physiological states. Here, we describe a powerful 7-day HSC hibernation culture system that maintains HSCs as single cells in the absence of a physical niche. Read More

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Characterization of Blue Light Treatment for Infected Wounds: Antibacterial Efficacy of 420, 455, and 480 nm Light-Emitting Diode Arrays Against Common Skin Pathogens Versus Blue Light-Induced Skin Cell Toxicity.

Photobiomodul Photomed Laser Surg 2021 May;39(5):339-348

Chair, Department of Virology and Microbiology, Centre for Biomedical Education and Research (ZBAF), University Witten/Herdecke, Witten, Germany.

To determine effective treatment strategies against bacterial infections of chronic wounds, we tested different blue light (BL)-emitting light-emitting diode arrays (420, 455, and 480 nm) against wound pathogens and investigated in parallel BL-induced toxic effects on human dermal fibroblasts. Wound infection is a major factor for delayed healing. Infections with and are clinically relevant caused by their ability of biofilm formation and their quickly growing antibiotics resistance. Read More

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Identification and characterisation of maternal perivascular SUSD2 placental mesenchymal stem/stromal cells.

Cell Tissue Res 2021 May 7. Epub 2021 May 7.

The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC, Australia.

Mesenchymal stem cells (MSCs) that meet the International Society for Cellular Therapy (ISCT) criteria are obtained from placental tissue by plastic adherence. Historically, no known single marker was available for isolating placental MSCs (pMSCs) from the decidua basalis. As the decidua basalis is derived from the regenerative endometrium, we hypothesised that SUSD2, an endometrial perivascular MSC marker, would purify maternal perivascular pMSC. Read More

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Analysis of Microbiological and Chemical Hazards in Edible Insects Available to Canadian Consumers.

J Food Prot 2021 May 6. Epub 2021 May 6.

Canadian Food Inspection Agency.

Edible insects are a novel food in most countries; their popularity is growing because of their high protein/low fat content, ease of cultivation, and small environmental impact. To our knowledge, this is the first report that addresses both microbiological and chemical hazards in edible insects. Samples were collected from retail stores or purchase through e-commerce. Read More

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Effects of isoquinoline alkaloids from Macleaya cordata on growth performance, survival, immune response, and resistance to Vibrio parahaemolyticus infection of Pacific white shrimp (Litopenaeus vannamei).

PLoS One 2021 6;16(5):e0251343. Epub 2021 May 6.

Phytobiotics (Thailand) Co., Ltd, Huaykwang, Bangkok, Thailand.

Isoquinoline alkaloids (IQs) from Macleaya cordata are promising natural products for enhancing the growth performance and overall health condition of farmed animals. The present study aimed to investigate the effects of two formulas of IQ extract, provided in either a powdered formula (IQ-E) or a water-soluble, granulated formula (IQ-WS) and containing the main active component sanguinarine at a concentration of 0.5% and 1%, respectively, on the growth, survival, immune response, and resistance to Vibrio parahaemolyticus infection of Pacific white shrimp (Litopenaeus vannamei). Read More

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Long noncoding RNA PP7080 promotes hepatocellular carcinoma development by sponging mir-601 and targeting SIRT1.

Bioengineered 2021 Dec;12(1):1599-1610

Department of Pathophysiology, Fenyang College of Shanxi Medical University, Fenyang, China.

Hepatocellular carcinoma (HCC) is the most common primary liver malignancy in adults, ranking the second leading cause of cancer-related death. To date, the underlying mechanisms of HCC pathogenesis are still unclear. Recently, more and more studies have reported that long noncoding RNAs (lncRNAs) are involved in the occurrence and development of HCC. Read More

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December 2021

Number and Replating Capacity of Endothelial Colony-Forming Cells are Telomere Length Dependent: Implication for Human Atherogenesis.

J Am Heart Assoc 2021 May 6:e020606. Epub 2021 May 6.

Inserm DCAC Université de Lorraine Nancy France.

Background Short leukocyte telomere length (TL) is associated with atherosclerotic cardiovascular disease. Endothelial repair plays a key role in the development of atherosclerosis. The objective was to examine associations between TL and proliferative dynamics of endothelial colony-forming cells (ECFCs), which behave as progenitor cells displaying endothelial repair activity. Read More

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LncRNA CCAT1 downregulation increases the radiosensitivity of non-small cell lung cancer cells.

Authors:
Zhao Wang

Kaohsiung J Med Sci 2021 May 6. Epub 2021 May 6.

Department of Oncology Radiotherapy 2, Yantai Yantaishan Hospital, Yantai, Shandong, China.

This study aims to investigate if the radiosensitivity of non-small cell lung cancer (NSCLC) cells can be regulated by long noncoding RNA (lncRNA) colon cancer associated transcript1 (CCAT1). CCAT1 was detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in NSCLC cells (A549, H1299, SK-MES1, H460, and H647) and human bronchial epithelial cells (16HBE). H460 and A549 cells were then selected for the determination of CCAT1 expression after exposure to radiation (0, 2, 4, 6 Gy) at different time points (0, 6, 12, 24 h). Read More

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TCEB3 is Regulated by Circ-0000212/miR-140-3p Axis to Promote the Progression of Cervical Cancer.

Onco Targets Ther 2021 28;14:2853-2865. Epub 2021 Apr 28.

Department of Geratology, The First Affiliated Hospital of Weifang Medical University (Weifang People's Hospital), Weifang, Shandong, 261041, People's Republic of China.

Background: Cervical cancer is a common female malignancy, which accounts for a large proportion of cancer-related mortality in the world. Therefore, exploring the mechanisms of cervical cancer progression and seeking new therapeutic targets are extraordinarily needful. The aim of this study was to explore the role of TCEB3 in cervical cancer progression. Read More

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Comparative genomics reveals an SNP potentially leading to phenotypic diversity of serovar Enteritidis.

Microb Genom 2021 May;7(5)

National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea.

An SNP is a spontaneous genetic change having a potential to modify the functions of the original genes and to lead to phenotypic diversity of bacteria in nature. In this study, a phylogenetic analysis of serovar Enteritidis, a major food-borne pathogen, showed that eight strains of . Enteritidis isolated in South Korea, including FORC_075 and FORC_078, have almost identical genome sequences. Read More

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Thymidine decreases the DNA damage and apoptosis caused by tumor-treating fields in cancer cell lines.

Genes Genomics 2021 May 5. Epub 2021 May 5.

School of Biosystems and Biomedical Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.

Background: Tumor-treating fields (TTFields) is an emerging non-invasive cancer-treatment modality using alternating electric fields with low intensities and an intermediate range of frequency. TTFields affects an extensive range of charged and polarizable cellular factors known to be involved in cell division. However, it causes side-effects, such as DNA damage and apoptosis, in healthy cells. Read More

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The Safety and Antimicrobial Properties of Stabilized Hypochlorous Acid in Acetic Acid Buffer for the Treatment of Acute Wounds-a Human Pilot Study and In Vitro Data.

Int J Low Extrem Wounds 2021 May 5:15347346211015656. Epub 2021 May 5.

SoftOx Solutions AS, Oslo, Norway.

Acute wounds may require cleansing to reduce the risk of infection. Stabilized hypochlorous acid in acetic buffer (HOCl + buffer) is a novel wound irrigation solution with antimicrobial properties. We performed a first-in-man, prospective, open-label pilot study to document preliminary safety and performance in the treatment of acute wounds. Read More

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Revisiting the inoculum effect for Streptococcus pyogenes with a hollow fibre infection model.

Eur J Clin Microbiol Infect Dis 2021 May 4. Epub 2021 May 4.

Faculty of Health and Medical Sciences, University of Western Australia, Perth, Western Australia, Australia.

Severe, invasive Streptococcus pyogenes (Strep A) infections result in greater than 500,000 deaths annually. First line treatment for such infections is benzylpenicillin, often with the addition of clindamycin, but treatment failure can occur with this regimen. This failure has been partially attributed to the inoculum effect, which presents as reduced antibiotic susceptibility during high bacterial density and plateau-phase growth. Read More

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Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton.

BMC Complement Med Ther 2021 May 4;21(1):136. Epub 2021 May 4.

Department of Oral and Maxillofacial Surgery, Facial Plastic Surgery, Rostock University Medical Center, Rostock, Germany.

Background: Prevention of metastatic invasion is one of the main challenges in the treatment of alveolar rhabdomyosarcoma. Still the therapeutic options are limited. Therefore, an anti-tumor screening was initiated focusing on the anti-metastatic and anti-invasion properties of selected medicinal plant extracts and phytoestrogens, already known to be effective in the prevention and treatment of different cancer entities. Read More

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Enzymatic Time-Temperature Indicator Prototype Developed by Immobilizing Laccase on Electrospun Fibers to Predict Lactic Acid Bacterial Growth in Milk during Storage.

Nanomaterials (Basel) 2021 Apr 29;11(5). Epub 2021 Apr 29.

Department of Food Science, National Ilan University, 1, Sec. 1, Shennong Rd., Yilan County, Yilan City 26047, Taiwan.

Laccase was immobilized on a chitosan/polyvinyl alcohol/tetraethylorthosilicate electrospun film (ceCPTL) and colored with guaiacol to obtain a laccase time-temperature indicator (TTI) prototype. The activation energy () of coloration of the prototype was 50.89-33. Read More

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Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer.

Sensors (Basel) 2021 Apr 30;21(9). Epub 2021 Apr 30.

Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan.

This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Read More

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Pseudomonas aeruginosa induces spatio-temporal secretion of IL-1β, TNFα, proMMP-9, and reduction of epithelial E-cadherin in human alveolar epithelial type II (A549) cells.

Acta Biochim Pol 2021 May 4. Epub 2021 May 4.

Department of Inmunobioquímica, Instituto Nacional de Perinatología "Isidro Espinoza de los Reyes" (INPerIER), Ciudad de México, México.

Pseudomonas aeruginosa, is an opportunistic bacterium with a high prevalence in diverse pulmonary infections. Although several genes are involved in the system of resistance and evasion of the immunological response of the host, little is known about the inflammatory, degradative, and cell-binding response induced by P. aeruginosa in human lung alveolar epithelial cells. Read More

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Polyester Mesh Functionalization with Nitric Oxide-Releasing Silica Nanoparticles Reduces Early Methicillin-Resistant Contamination.

Surg Infect (Larchmt) 2021 Apr 30. Epub 2021 Apr 30.

Houston Methodist Research Institute, Houston, Texas, USA.

Infected hernia mesh is a cause of post-operative morbidity. Nitric oxide (NO) plays a key role in the endogenous immune response to infection. We sought to study the efficacy of a NO-releasing mesh against methicillin-resistant (MRSA). Read More

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First Report of Causing Stem Canker of Eggplant in Fujian Province, China.

Plant Dis 2021 May 4. Epub 2021 May 4.

Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, P. R. China, Fuzhou, Fujian province, China, 350002;

Eggplant ( L.) is one of the most popular vegetable in China. In July 2019, a serious stem canker disease of eggplant cv. Read More

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Evaluation of graded levels of Bacillus coagulans GBI-30, 6086 on apparent nutrient digestibility, stool quality, and intestinal health indicators in healthy adult dogs.

J Anim Sci 2021 May 3. Epub 2021 May 3.

Department of Grain Science and Industry, Kansas State University, Manhattan, KS, USA.

Bacillus coagulans GBI-30, 6086 is a commercially available spore-forming non-toxigenic microorganism approved for use in dog foods with high resiliency to stresses associated with commercial manufacturing. The objectives of this research were to examine the effect of Bacillus coagulans on stool quality, nutrient digestibility, and intestinal health markers in healthy adult dogs. Extruded diets containing graded levels of Bacillus coagulans applied either to the base ration before extrusion or to the exterior of the kibble as a topical coating after extrusion were randomly assigned to ten individually housed adult beagle dogs (7 castrated males, 3 spayed females) of similar age (5. Read More

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Mobilized Peripheral Blood Stem Cells are Pluripotent and Can be Safely Harvested and Stored for Cartilage Repair.

Arthroscopy 2021 Apr 30. Epub 2021 Apr 30.

Kuala Lumpur Sports Medicine Centre, 47, Jalan Dungun, Bukit Damansara Kuala Lumpur, Malaysia.

Purpose: The primary objective of this study was to reproduce and validate the harvest, processing and storage of peripheral blood stem cells for a subsequent cartilage repair trial, evaluating safety, reliability, and potential to produce viable, sterile stem cells.

Methods: Ten healthy subjects (aged 19-44 years) received three consecutive daily doses of filgrastim followed by an apheresis harvest of mononuclear cells on a fourth day. In a clean room, the apheresis product was prepared for cryopreservation and processed into 4mL aliquots. Read More

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