80,850 results match your criteria colony formation


Impacts of Magnetic Immobilization on the Growth and Metabolic Status of Recombinant Pichia pastoris.

Mol Biotechnol 2021 Oct 13. Epub 2021 Oct 13.

Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Downstream processing is an expensive step for industrial production of recombinant proteins. Cell immobilization is known as one of the ideal solutions in regard to process intensification. In recent years, magnetic immobilization was introduced as a new technique for cell immobilization. Read More

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October 2021

RFWD2 Knockdown as a Blocker to Reverse the Oncogenic Role of TRIB2 in Lung Adenocarcinoma.

Front Oncol 2021 27;11:733175. Epub 2021 Sep 27.

Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, China.

RFWD2, an E3 ubiquitin ligase, is overexpressed in numerous human cancers, including leukemia, lung cancer, breast cancer, renal cell carcinoma, and colorectal cancer. The roles of RFWD2 in cancer are related to the targeting of its substrates for ubiquitination and degradation. This study aimed to investigate the role of TRIB2 in relation to the regulation of protein degradation through RFWD2. Read More

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September 2021

Novel mutation causing propionic acidemia associated with unexplained autoimmune thyrotoxicosis.

Mol Genet Metab Rep 2021 Dec 4;29:100806. Epub 2021 Oct 4.

Division of Metabolic and Genetics, Department of Pediatrics, Sheikh Khalifa Medical City, Abu Dhabi, United Arab Emirates.

Propionic acidemia (PA) is a rare autosomal recessive inborn error of metabolism (IEM) with relatively higher prevalence in the United Arab Emirates (UAE). Absence of propionyl-CoA carboxylase (PCC) enzyme classically leads to acute decompensation in the early neonatal period. We report a novel homozygous frameshift variant Glu720Valfs*14 (NM_000282. Read More

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December 2021

Fascin-1 is Highly Expressed Specifically in Microglia After Spinal Cord Injury and Regulates Microglial Migration.

Front Pharmacol 2021 27;12:729524. Epub 2021 Sep 27.

Department of Orthopaedics, The Second Hospital of Anhui Medical University, Hefei, China.

Recent research indicates that after spinal cord injury (SCI), microglia accumulate at the borders of lesions between astrocytic and fibrotic scars and perform inflammation-limiting and neuroprotective functions, however, the mechanism of microglial migration remains unclear. Fascin-1 is a key actin-bundling protein that regulates cell migration, invasion and adhesion, but its role during SCI has not been reported. Here, we found that at 7-14 days after SCI in mice, Fascin-1 is significantly upregulated, mainly distributed around the lesion, and specifically expressed in CX3CR1-positive microglia. Read More

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September 2021

Antiproliferative Effect of Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells.

ScientificWorldJournal 2021 4;2021:3752169. Epub 2021 Oct 4.

Department of Pharmacy Practice, College of Pharmacy, Rangsit University, Lak Hok, Pathum Thani 12000, Thailand.

/. Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. Read More

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October 2021

DRAIC promotes growth of breast cancer by sponging miR-432-5p to upregulate SLBP.

Cancer Gene Ther 2021 Oct 13. Epub 2021 Oct 13.

Department of Breast Surgery, the First Hospital of Jilin University, Changchun, 130021, Jilin, China.

Mounting evidence suggests that lncRNAs can exert functions in cancer progression in multiple manners. In recent years, competing endogenous RNA (ceRNA) has been widely reported in human cancers as a lncRNA-dominant molecular pathway. The current study aimed at proving the role of lncRNA downregulated RNA in cancer (DRAIC) in breast cancer (BRCA) progression. Read More

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October 2021

Tryptophan plays an important role in yeast's tolerance to isobutanol.

Biotechnol Biofuels 2021 Oct 13;14(1):200. Epub 2021 Oct 13.

Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taipei, Taiwan.

Background: Isobutanol is considered a potential biofuel, thanks to its high-energy content and octane value, limited water solubility, and compatibility with gasoline. As its biosynthesis pathway is known, a microorganism, such as Saccharomyces cerevisiae, that inherently produces isobutanol, can serve as a good engineering host. Isobutanol's toxicity, however, is a major obstacle for bioproduction. Read More

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October 2021

An SETD1A/Wnt/β-catenin feedback loop promotes NSCLC development.

J Exp Clin Cancer Res 2021 Oct 13;40(1):318. Epub 2021 Oct 13.

Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 277 Yanta West Road, Xi'an, 710061, Shaanxi Province, China.

Background: SETD1A, a member of SET1/MLL family H3K4 methyltransferases, is involved in the tumorigenesis of numerous cancers. However, the biological role and mechanism of SETD1A in non-small cell lung cancer (NSCLC) remain to be elucidated.

Methods: The expression of SETD1A, NEAT1, EZH2, and β-catenin in NSCLC tissues and cell lines was detected by qRT-PCR, immunohistochemistry and western blotting. Read More

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October 2021

Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p.

World J Surg Oncol 2021 Oct 14;19(1):299. Epub 2021 Oct 14.

Cancer Center, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No.1 Jingba Road, Shizhong District, Jinan City, 250001, Shandong Province, China.

Background: Emerging evidence reveals that the initiation and development of human cancers, including colorectal cancer (CRC), are associated with the deregulation of circular RNAs (circRNAs). Our study intended to disclose the role of circ_0026416 in the malignant behaviors of CRC.

Methods: The detection for circ_0026416 expression, miR-545-3p expression, and myosin VI (MYO6) mRNA expression was performed using quantitative real-time PCR (qPCR). Read More

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October 2021

Anticancer effects of 6-shogaol via the AKT signaling pathway in oral squamous cell carcinoma.

J Appl Oral Sci 2021 11;29:e20210209. Epub 2021 Oct 11.

Kyungpook National University, Department of Dental Hygiene, Sangju, Republic of Korea.

Objective: Oral squamous cell carcinoma (OSCC) is one of the common type of cancer that leads to death; and is becoming a global concern. Due to the lack of efficient chemotherapeutic agents for patients with oral cancer, the prognosis remains poor. 6-shogaol, a bioactive compound of ginger, has a broad spectrum of bioactivities and has been widely used to relieve many diseases. Read More

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October 2021

Pevonedistat targets malignant cells in myeloproliferative neoplasms in vitro and in vivo via NFkB pathway inhibition.

Blood Adv 2021 Oct 13. Epub 2021 Oct 13.

Washington University School of Medicine, St. Louis, Missouri, United States.

Targeted inhibitors of JAK2 (e.g. ruxolitinib) often provide symptomatic relief for myeloproliferative neoplasm (MPN) patients, but the malignant clone persists and remains susceptible to disease transformation. Read More

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October 2021

Doxycycline sensitizes renal cell carcinoma to chemotherapy by preferentially inhibiting mitochondrial translation.

J Int Med Res 2021 Oct;49(10):3000605211044368

Department of Urology, The First People's Hospital of Jiangxia District Wuhan City, Wuhan, China.

Objectives: The anti-cancer activity of doxycycline has been reported in many cancers but not renal cell carcinoma (RCC). This study aimed to determine the efficacy of doxycycline alone and in combination with paclitaxel and analyze the underlying mechanism in RCC.

Methods: Proliferation, colony formation and apoptosis assays were performed in RCC cell lines after drug treatments. Read More

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October 2021

G-CSF and IL-6 may be involved in formation of endometriosis lesions by increasing the expression of angiogenic factors in neutrophils.

Mol Hum Reprod 2021 Oct 13. Epub 2021 Oct 13.

Department of Obstetrics and Gynaecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, The People's Republic of China.

Evidence accumulated in recent years has revealed that neutrophils are involved in the initial establishment of endometriosis, which is well known as a chronic inflammatory disease. So far, why and how neutrophils promote the formation of early endometriosis are still unclear. In this study, using a mouse model of endometriosis, we demonstrated that endometriosis mice (EMs mice) had a significantly increased number of neutrophils in peritoneal fluids and lesions, and increased levels of granulocyte colony stimulating factor (G-CSF) and IL-6 in serum and peritoneal fluids compared to the control group. Read More

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October 2021

Schizandrin A can inhibit non‑small cell lung cancer cell proliferation by inducing cell cycle arrest, apoptosis and autophagy.

Int J Mol Med 2021 Dec 13;48(6). Epub 2021 Oct 13.

Department of Thoracic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.

Schizandrin A (SchA) can be extracted from the vine plant and has been reported to confer various biologically active properties. However, its potential biological effects on non‑small cell lung cancer (NSCLC) remain unknown. Therefore, the present study aims to address this issue. Read More

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December 2021

Berberine exerts its antineoplastic effects by reversing the Warburg effect via downregulation of the Akt/mTOR/GLUT1 signaling pathway.

Oncol Rep 2021 Dec 13;46(6). Epub 2021 Oct 13.

Department of Plant Sciences, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, P.R. China.

Glucose transporter 1 (GLUT1) plays a primary role in the glucose metabolism of cancer cells. However, to the best of our knowledge, there are currently no anticancer drugs that inhibit GLUT1 function. The present study aimed to investigate the antineoplastic activity of berberine (BBR), the main active ingredient in numerous Traditional Chinese medicinal herbs, on HepG2 and MCF7 cells. Read More

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December 2021

Tissue-specific transcriptional patterns underlie seasonal phenotypes in honey bees (Apis mellifera).

Mol Ecol 2021 Oct 12. Epub 2021 Oct 12.

Department of Entomology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University.

Faced with adverse conditions, such as winter in temperate regions or hot and dry conditions in tropical regions, many insect species enter a state of diapause, a period of dormancy associated with a reduction or arrest of physical activity, development, and reproduction. Changes in common physiological pathways underlie diapause phenotypes in different insect species. However, most transcriptomic studies of diapause have not simultaneously evaluated and compared expression patterns in different tissues. Read More

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October 2021

Podophyllotoxin Induces ROS-Mediated Apoptosis and Cell Cycle Arrest in Human Colorectal Cancer Cells via p38 MAPK Signaling.

Biomol Ther (Seoul) 2021 Oct 13. Epub 2021 Oct 13.

Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan 58554, Republic of Korea.

Podophyllotoxin (PT), a lignan compound from the roots and rhizomes of , has diverse pharmacological activities including anticancer effect in several types of cancer. The molecular mechanism of the anticancer effects of PT on colorectal cancer cells has not been reported yet. In this study, we sought to evaluate the anticancer effect of PT on human colorectal cancer HCT116 cells and identify the detailed molecular mechanism. Read More

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October 2021

Exploring microwave irradiation as a method to disinfect contact lens cases.

Cont Lens Anterior Eye 2021 Oct 9:101522. Epub 2021 Oct 9.

Department of Mechanical Engineering, College of Engineering, University of Kentucky, 506 Administration Drive, Lexington, KY, 40506, United States. Electronic address:

Purpose: Biofilm formation caused by infrequent contact lens case replacement and the ineffectiveness of multi-purpose solutions (MPS) on biofilm removal is associated with high rates of bacterial keratitis infections. This study demonstrated biofilm elimination from the contact lens case by microwave irradiation.

Methods: Staphylococcus aureus biofilms indicative of 3-9 months of contact wear were cultured in contact lens cases and visualized with crystal violet (CV) staining. Read More

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October 2021

Circular RNA hsa_circ_0002483 promotes growth and invasion of lung adenocarcinoma by sponging miR-125a-3p.

Cancer Cell Int 2021 Oct 12;21(1):533. Epub 2021 Oct 12.

Department of Thoracic Surgery, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, 518020, China.

Background: Increasing evidence indicates that the aberrant expression of circular RNAs (circRNAs) is involved in the pathogenesis and progression of lung adenocarcinoma (LUAC). However, the function and molecular mechanisms of hsa_circ_0002483 (circ_0002483) in LUAC remain unclear.

Methods: The association between circ_0002483 expression and clinicopathological characteristics and prognosis in patients with LUAC was analyzed by fluorescence in situ hybridization. Read More

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October 2021

Analyzing the whole-transcriptome profiles of ncRNAs and predicting the competing endogenous RNA networks in cervical cancer cell lines with cisplatin resistance.

Cancer Cell Int 2021 Oct 12;21(1):532. Epub 2021 Oct 12.

Department of Obstetrics and Gynecology, Third Hospital of Shanxi Medical University (Shanxi Bethune Hospital), Shanxi Academy of Medical Sciences, TaiYuan, 030032, China.

Background: Cervical cancer (CC) is one of the most common malignant tumors in women. In order to identify the functional roles and the interaction between mRNA and non-coding RNA (ncRNA, including lncRNA, circRNA and miRNA) in CC cisplatin (DDP) resistance, the transcription profile analysis was performed and a RNA regulatory model of CC DDP resistance was proposed.

Methods: In this study, whole-transcriptome sequencing analysis was conducted to study the ncRNA and mRNA profiles of parental SiHa cells and DDP resistant SiHa/DDP cells. Read More

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October 2021

EF24 exerts cytotoxicity against NSCLC via inducing ROS accumulation.

Cancer Cell Int 2021 Oct 12;21(1):531. Epub 2021 Oct 12.

Physical and Chemical Laboratory, Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, 250000, People's Republic of China.

Background: The role of Diphenyldifluoroketone (EF24), a synthetic analogue of curcumin with noteworthy antitumor potential, remains unclear in non-small cell lung cancer (NSCLC). Herein, the inhibitory effect of EF24 on NSCLC and its mechanism were studied.

Methods: Cytotoxicity was measured by MTT assay, colony formation assay and xenograft model. Read More

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October 2021

The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation.

Molecules 2021 Oct 2;26(19). Epub 2021 Oct 2.

Biophysical Research Group, Vytautas Magnus University, Vileikos st. 844404, LT-44001 Kaunas, Lithuania.

One of current applications of electroporation is electrochemotherapy and electroablation for local cancer treatment. Both of these electroporation modalities share some similarities with radiation therapy, one of which could be the bystander effect. In this study, we aimed to investigate the role of the bystander effect following these electroporation-based treatments. Read More

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October 2021

Juglone Inactivates through Cell Membrane Damage, Biofilm Blockage, and Inhibition of Gene Expression.

Molecules 2021 Sep 27;26(19). Epub 2021 Sep 27.

College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an 710119, China.

Due to the strong drug resistance of (), the inhibition effects of conventional disinfectants and antibiotics are not obvious. Juglone extracted from discarded walnut husk, as a kind of plant-derived antimicrobial agent, has the advantages of naturalness, high efficiency, and low residue, with a potential role in the inhibition of . This study elucidated the inhibitory effect of juglone on the growth of plankton and the formation of biofilm. Read More

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September 2021

Time Evolution of Microbial Composition and Metabolic Profile for Biogenic Amines and Free Amino Acids in a Model Cucumber Fermentation System Brined with 0.5% to 5.0% Sodium Chloride.

Molecules 2021 Sep 24;26(19). Epub 2021 Sep 24.

Department of Food Safety and Chemical Analysis, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology-State Research Institute, Rakowiecka 36 St., 02-532 Warsaw, Poland.

Salt concentrations in brine and temperature are the major environmental factors that affect activity of microorganisms and, thus may affect formation of biogenic amines (BAs) during the fermentation process. A model system to ferment cucumbers with low salt (0.5%, 1. Read More

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September 2021

Synthesis, Anti-Proliferative Evaluation and Mechanism of 4-Trifluoro Methoxy Proguanil Derivatives with Various Carbon Chain Length.

Molecules 2021 Sep 24;26(19). Epub 2021 Sep 24.

Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, Key Laboratory of Protein Chemistry and Developmental Biology of Fish of Ministry of Education, Department of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410205, China.

Among the known biguanide drugs, proguanil has the best antiproliferative activity. In contrast, newly synthesized biguanide derivatives containing fluorine atoms have excellent biological activity, among which trifluoromethoxy compounds show the strongest ability. Preliminary work in our laboratory exhibited that n-heptyl containing proguanil derivatives on one alkyl chain side have better biological activity than those with a shorter carbon chain. Read More

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September 2021

Effects of Modified Clay on Growth and Colony Formation.

Int J Environ Res Public Health 2021 Sep 27;18(19). Epub 2021 Sep 27.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

is a globally distributed harmful algal blooms (HABs) species dominated by the colonial morphotype, which presents dramatic environmental hazards and poses a threat to human health. Modified clay (MC) can effectively flocculate HAB organisms and prevent their subsequent growth, but the effects of MC on colony-dominated blooms remain uncertain. In this paper, a series of removal and incubation experiments were conducted to investigate the growth, colony formation and colony development of cells after treatment with MC. Read More

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September 2021

MS-275 (Entinostat) Promotes Radio-Sensitivity in PAX3-FOXO1 Rhabdomyosarcoma Cells.

Int J Mol Sci 2021 Oct 1;22(19). Epub 2021 Oct 1.

Department of Radiotherapy, Policlinico Umberto I, Sapienza University of Rome, 00161 Rome, Italy.

Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood. About 25% of RMS expresses fusion oncoproteins such as PAX3/PAX7-FOXO1 (fusion-positive, FP) while fusion-negative (FN)-RMS harbors RAS mutations. Radiotherapy (RT) plays a crucial role in local control but metastatic RMS is often radio-resistant. Read More

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October 2021

Metformin Affects Olaparib Sensitivity through Induction of Apoptosis in Epithelial Ovarian Cancer Cell Lines.

Int J Mol Sci 2021 Sep 29;22(19). Epub 2021 Sep 29.

Department of Medical Biophysics, Faculty of Biology and Environmental Protection, Institute of Biophysics, University of Lodz, 90-236 Lodz, Poland.

This study examined the effect of combination treatment with the poly (ADP-ribose) polymerase inhibitor olaparib and metformin on homologous recombination (HR)-proficient epithelial ovarian cancer (EOC). Ovarian cancer cell lines (OV-90 and SKOV-3) were treated with olaparib, metformin, or a combination of both. Cell viability was assessed by MTT and colony formation assays. Read More

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September 2021

Interleukin-9 Facilitates Osteoclastogenesis in Rheumatoid Arthritis.

Int J Mol Sci 2021 Sep 27;22(19). Epub 2021 Sep 27.

Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi 110029, India.

In rheumatoid arthritis (RA), inflammatory cytokines play a pivotal role in triggering abnormal osteoclastogenesis leading to articular destruction. Recent studies have demonstrated enhanced levels of interleukin-9 (IL-9) in the serum and synovial fluid of patients with RA. In RA, strong correlation has been observed between tissue inflammation and IL-9 expression in synovial tissue. Read More

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September 2021

Delineating the Switch between Senescence and Apoptosis in Cervical Cancer Cells under Ciclopirox Treatment.

Cancers (Basel) 2021 Oct 5;13(19). Epub 2021 Oct 5.

Molecular Therapy of Virus-Associated Cancers, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.

The iron-chelating drug ciclopirox (CPX) may possess therapeutic potential for cancer treatment, including cervical cancer. As is observed for other chemotherapeutic drugs, CPX can induce senescence or apoptosis in cervical cancer cells which could differently affect their therapy response. The present study aims to gain insights into the determinants which govern the switch between senescence and apoptosis in cervical cancer cells. Read More

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October 2021