120 results match your criteria collagen-based dermal


Poly(lactide-co-ε-caprolactone) scaffold promotes equivalent tissue integration and supports skin grafts compared to a predicate collagen scaffold.

Wound Repair Regen 2021 Jun 15. Epub 2021 Jun 15.

Division of Plastic and Reconstructive Surgery, Department of Surgery, School of Medicine, Duke University, Durham, North Carolina, USA.

Dermal scarring from motor vehicle accidents, severe burns, military blasts, etc. is a major problem affecting over 80 million people worldwide annually, many of whom suffer from debilitating hypertrophic scar contractures. These stiff, shrunken scars limit mobility, impact quality of life, and cost millions of dollars each year in surgical treatment and physical therapy. Read More

View Article and Full-Text PDF

Highly stable collagen scaffolds crosslinked with an epoxidized natural polysaccharide for wound healing.

Int J Biol Macromol 2021 Jul 29;182:1994-2002. Epub 2021 May 29.

National Engineering Research Center for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China; The Research Center of Biomedicine Engineering of Sichuan University, Chengdu 610065, China.

As a biocompatible and bioactive natural tissue engineering collagen scaffold, porcine acellular dermal matrix (pADM) has limitations for the application in tissue regeneration due to its low strength and rapid biodegradation. Herein, to get a good wound dressing, the epoxy group was added to N-(2-hydroxypropyl)-3-trimethylammonium chitosan chloride (HTCC) to synthesize the epoxidized N-(2-hydroxypropyl)-3-trimethylammonium chitosan chloride (EHTCC), and the porcine acellular dermal matrix was modified with EHTCC at different dosage of 0, 4, 8, 12, 16 and 20%. The properties of the EHTCC-pADM were evaluated. Read More

View Article and Full-Text PDF

Association of Systemic or Intravitreal Antivascular Endothelial Growth Factor (Anti-VEGF) and Impaired Wound Healing in Pediatric Patients: Collagen to the Rescue.

Authors:
Vita Boyar

J Wound Ostomy Continence Nurs 2021 May-Jun 01;48(3):256-261

Vita Boyar, MD, Steven & Alexandra Cohen Children's Medical Center, New Hyde Park, New York; and Zucker School of Medicine at Hofstra/Northwell, New Hyde Park, New York.

Background: Bevacizumab is a humanized monoclonal antibody to vascular endothelial growth factor (VEGF) that has been used as a systemic chemotherapy treatment of various malignancies in adults since 2000. It has been used for pediatric patients over the last decade. In addition, bevacizumab is used for neonatal intravitreal administration for retinopathy of prematurity, a major complication of preterm birth, characterized by incomplete and abnormal vascularization of the retina that can lead to retinal detachment and blindness without treatment. Read More

View Article and Full-Text PDF

Use of photosynthetic transgenic cyanobacteria to promote lymphangiogenesis in scaffolds for dermal regeneration.

Acta Biomater 2021 05 20;126:132-143. Epub 2021 Mar 20.

Division of Hand, Plastic and Aesthetic Surgery, University Hospital, LMU Munich, Munich, Germany; Frauenklinik Dr. Geisenhofer, Munich, Germany. Electronic address:

Impaired wound healing represents an unsolved medical need with a high impact on patients´ quality of life and global health care. Even though its causes are diverse, ischemic-hypoxic conditions and exacerbated inflammation are shared pathological features responsible for obstructing tissue restoration. In line with this, it has been suggested that promoting a normoxic pro-regenerative environment and accelerating inflammation resolution, by reinstating the lymphatic fluid transport, could allow the wound healing process to be resumed. Read More

View Article and Full-Text PDF

Biological testing of chitosan-collagen-based porous scaffolds loaded with PLGA/Triamcinolone microspheres for ameliorating endoscopic dissection-related stenosis in oesophagus.

Cell Prolif 2021 Mar 4;54(3):e13004. Epub 2021 Feb 4.

Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, China.

Objectives: Endoscopic submucosal dissection (ESD), a preferential approach for early oesophageal neoplasms, inevitably results in oesophageal strictures in patients. Clinical use of glucocorticoids through submucosal injection is beneficial for inhibiting oesophageal stricture following injury; however, it also has limitations, such as dose loss and perforation. Hence, alternatives to glucocorticoid therapy should be developed. Read More

View Article and Full-Text PDF

Skin regeneration is accelerated by a lower dose of multipotent mesenchymal stromal/stem cells-a paradigm change.

Stem Cell Res Ther 2021 01 25;12(1):82. Epub 2021 Jan 25.

Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, Canada.

Background: Multipotent mesenchymal stromal/stem cell (MSC) therapy is under investigation in promising (pre-)clinical trials for wound healing, which is crucial for survival; however, the optimal cell dosage remains unknown. The aim was to investigate the efficacy of different low-to-high MSC dosages incorporated in a biodegradable collagen-based dermal regeneration template (DRT) Integra®.

Methods: We conducted a porcine study (N = 8 Yorkshire pigs) and seeded between 200 and 2,000,000 cells/cm of umbilical cord mesenchymal stromal/stem cells on the DRT and grafted it onto full-thickness burn excised wounds. Read More

View Article and Full-Text PDF
January 2021

The influence of animal species, gender and tissue on the structural, biophysical, biochemical and biological properties of collagen sponges.

J Mater Sci Mater Med 2021 Jan 21;32(1):12. Epub 2021 Jan 21.

Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.

Although collagen type I is extensively used in biomedicine, no study to-date has assessed how the properties of the produced scaffolds are affected as a function of species, gender and tissue from which the collagen was extracted. Herein, we extracted and characterised collagen from porcine and bovine, male and female and skin and tendon tissues and we subsequently fabricated and assessed the structural, biophysical, biochemical and biological properties of collagen sponges. All collagen preparations were of similar purity and free-amine content (p > 0. Read More

View Article and Full-Text PDF
January 2021

Alginate Affects Bioactivity of Chimeric Collagen-Binding LL37 Antimicrobial Peptides Adsorbed to Collagen-Alginate Wound Dressings.

ACS Biomater Sci Eng 2020 06 21;6(6):3398-3410. Epub 2020 May 21.

Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, United States.

Chronic infected wounds cause more than 23,000 deaths annually. Antibiotics and antiseptics are conventionally used to treat infected wounds; however, they can be toxic to mammalian cells, and their use can contribute to antimicrobial resistance. Antimicrobial peptides (AMPs) have been utilized to address the limitations of antiseptics and antibiotics. Read More

View Article and Full-Text PDF

3D printing of bioreactors in tissue engineering: A generalised approach.

PLoS One 2020 30;15(11):e0242615. Epub 2020 Nov 30.

Department Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, Würzburg, Germany.

3D printing is a rapidly evolving field for biological (bioprinting) and non-biological applications. Due to a high degree of freedom for geometrical parameters in 3D printing, prototype printing of bioreactors is a promising approach in the field of Tissue Engineering. The variety of printers, materials, printing parameters and device settings is difficult to overview both for beginners as well as for most professionals. Read More

View Article and Full-Text PDF
January 2021

Processed Eggshell Membrane Powder Is a Promising Biomaterial for Use in Tissue Engineering.

Int J Mol Sci 2020 Oct 30;21(21). Epub 2020 Oct 30.

Nofima AS, Pb 210, NO-1431 Ås, Norway.

The purpose of this study was to investigate the tissue regenerating and biomechanical properties of processed eggshell membrane powder (PEP) for use in 3D-scaffolds. PEP is a low-cost, natural biomaterial with beneficial bioactive properties. Most importantly, this material is available as a by-product of the chicken egg processing (breaking) industry on a large scale, and it could have potential as a low-cost ingredient for therapeutic scaffolds. Read More

View Article and Full-Text PDF
October 2020

Engineered PLGA-PVP/VA based formulations to produce electro-drawn fast biodegradable microneedles for labile biomolecule delivery.

Prog Biomater 2020 Dec 3;9(4):203-217. Epub 2020 Nov 3.

Center for Advanced Biomaterials for Health Care, CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125, Naples, Italy.

Biodegradable polymer microneedles (MNs) are recognized as non-toxic, safe and stable systems for advanced drug delivery and cutaneous treatments, allowing a direct intradermal delivery and in some cases a controlled release. Most of the microneedles found in the literature are fabricated by micromolding, which is a multistep thus typically costly process. Due to industrial needs, mold-free methods represent a very intriguing approach in microneedle fabrication. Read More

View Article and Full-Text PDF
December 2020

Full-thickness tissue engineered oral mucosa for genitourinary reconstruction: A comparison of different collagen-based biodegradable membranes.

J Biomed Mater Res B Appl Biomater 2021 Apr 10;109(4):572-583. Epub 2020 Sep 10.

Department of Gynecology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.

Tissue engineering is a method of growing importance regarding clinical application in the genitourinary region. One of the key factors in successfully development of an artificially tissue engineered mucosa equivalent (TEOM) is the optimal choice of the scaffold. Collagen scaffolds are regarded as gold standard in dermal tissue reconstruction. Read More

View Article and Full-Text PDF

Preparation and Characterization of Electrospun Collagen Based Composites for Biomedical Applications.

Materials (Basel) 2020 Sep 7;13(18). Epub 2020 Sep 7.

Physics of Polymers and Polymeric Materials, "Petru Poni" Institute of Macromolecular Chemistry, Aleea Grigore Ghica Voda, 41A, 700487 Iasi, Romania.

Electrospinning is a widely used technology for obtaining nanofibers from synthetic and natural polymers. In this study, electrospun mats from collagen (C), polyethylene terephthalate (PET) and a blend of the two (C-PET) were prepared and stabilized through a cross-linking process. The aim of this research was to prepare and characterize the nanofiber structure by Fourier-transform infrared with attenuated total reflectance spectroscopy (FTIR-ATR) in close correlation with dynamic vapor sorption (DVS). Read More

View Article and Full-Text PDF
September 2020

A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing.

Stem Cell Res Ther 2020 09 7;11(1):383. Epub 2020 Sep 7.

MOE Key Laboratory of Laser Life Science, College of Biophotonics & Institute of Laser Life Science, South China Normal University, Guangzhou, 510631, China.

Background: Stem cell therapies have gained great attention for providing novel solutions for treatment of various injuries and diseases due to stem cells' self-renewal, ability to differentiate into various cell types, and favorite paracrine function. Nevertheless, the low retention of transplanted stem cell still limits their clinical applications such as in wound healing in view of an induced harsh microenvironment rich in reactive oxygen species (ROS) during inflammatory reactions.

Methods: Herein, a novel chitosan/acellular dermal matrix (CHS/ADM) stem cell delivery system is developed, which is of great ROS scavenging activity and significantly attenuates inflammatory response. Read More

View Article and Full-Text PDF
September 2020

Enhanced Wound Healing Potential of Primary Human Oral Fibroblasts and Periodontal Ligament Cells Cultured on Four Different Porcine-Derived Collagen Matrices.

Materials (Basel) 2020 Aug 29;13(17). Epub 2020 Aug 29.

Laboratory of Oral Cell Biology, Dental Research Center, School of Dental Medicine, University of Bern, Freiburgstrasse 3, 3010 Bern, Switzerland.

Xenogenic collagen-based matrices represent an alternative to subepithelial palatal connective tissue autografts in periodontal and peri-implant soft tissue reconstructions. In the present study, we aimed to investigate the migratory, adhesive, proliferative, and wound-healing potential of primary human oral fibroblasts (hOF) and periodontal ligament cells (hPDL) in response to four commercially available collagen matrices. Non-crosslinked collagen matrix (NCM), crosslinked collagen matrix (CCM), dried acellular dermal matrix (DADM), and hydrated acellular dermal matrix (HADM) were all able to significantly enhance the ability of hPDL and hOF cells to directionally migrate toward the matrices as well as to efficiently repopulate an artificially generated wound gap covered by the matrices. Read More

View Article and Full-Text PDF

From Food Waste to Innovative Biomaterial: Sea Urchin-Derived Collagen for Applications in Skin Regenerative Medicine.

Mar Drugs 2020 Aug 6;18(8). Epub 2020 Aug 6.

Department of Environmental Science and Policy, University of Milan, Via Celoria, 2, 20133 Milan, Italy.

Collagen-based skin-like scaffolds (CBSS) are promising alternatives to skin grafts to repair wounds and injuries. In this work, we propose that the common marine invertebrate sea urchin represents a promising and eco-friendly source of native collagen to develop innovative CBSS for skin injury treatment. Sea urchin food waste after gonad removal was here used to extract fibrillar glycosaminoglycan (GAG)-rich collagen to produce bilayer (2D + 3D) CBSS. Read More

View Article and Full-Text PDF

Adipose-Derived Stromal Cells Seeded on Integra Dermal Regeneration Template Improve Post-Burn Wound Reconstruction.

Bioengineering (Basel) 2020 Jul 2;7(3). Epub 2020 Jul 2.

Cell Bank, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.

Fibrosis of burn-related wounds remains an unresolved clinical issue that leads to patient disability. The aim of this study was to assess the efficacy of the transplantation of adipose-derived stromal cells seeded onto a collagen-based matrix in the reconstruction of burn-related scars. Here, we characterized an in vitro interaction between adipose-derived stromal cells and a collagen-based matrix, IntegraDRT. Read More

View Article and Full-Text PDF

A Comparative Study of Engineered Dermal Templates for Skin Wound Repair in a Mouse Model.

Int J Mol Sci 2020 Jun 25;21(12). Epub 2020 Jun 25.

Skin Bioengineering Laboratory, Victorian Adult Burns Service, Alfred Health, 89 Commercial Road, Melbourne VIC 3004, Australia.

Engineered dermal templates have revolutionised the repair and reconstruction of skin defects. Their interaction with the wound microenvironment and linked molecular mediators of wound repair is still not clear. This study investigated the wound bed and acellular "off the shelf" dermal template interaction in a mouse model. Read More

View Article and Full-Text PDF

Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers.

Biomaterials 2020 09 22;254:120109. Epub 2020 May 22.

Cambridge Centre for Medical Materials, Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge, CB3 0FS, United Kingdom. Electronic address:

Due to its ubiquity and versatility in the human body, collagen is an ideal base material for tissue-engineering constructs. Chemical crosslinking treatments allow precise control of the biochemical and mechanical properties through macromolecular modifications to the structure of collagen. In this work, three key facets regarding the collagen crosslinking process are explored. Read More

View Article and Full-Text PDF
September 2020

Mesenchymal stromal cells from dermal and adipose tissues induce macrophage polarization to a pro-repair phenotype and improve skin wound healing.

Cytotherapy 2020 05 29;22(5):247-260. Epub 2020 Mar 29.

Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil; National Institute of Science and Technology for Regenerative Medicine, Rio de Janeiro, Brazil. Electronic address:

The process of wound healing restores skin homeostasis but not full functionality; thus, novel therapeutic strategies are needed to accelerate wound closure and improve the quality of healing. In this context, tissue engineering and cellular therapies are promising approaches. Although sharing essential characteristics, mesenchymal stromal cells (MSCs) isolated from different tissues might have distinct properties. Read More

View Article and Full-Text PDF

Application of Dermal Skin Substitutes for Hand and Finger Palmar Soft Tissue Loss.

Plast Reconstr Surg Glob Open 2019 Nov 27;7(11):e2551. Epub 2019 Nov 27.

Plastic and Reconstructive Surgery Department, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

Restoring function after traumatic defects of the palm is a reconstructive challenge, considering the need for flexible, elastic, and resistant skin. Dermal skin substitutes are biologically engineered materials composed of collagen and glycosaminoglycan, devoid of cellular structures. These biodegradable materials act as artificial dermis and stimulate neovascularization: they have been used for many years, mainly on the dorsal side of the hand and fingers, whereas the palmar side of the hand has been generally addressed by local flaps. Read More

View Article and Full-Text PDF
November 2019

Functionalising Collagen-Based Scaffolds With Platelet-Rich Plasma for Enhanced Skin Wound Healing Potential.

Front Bioeng Biotechnol 2019 3;7:371. Epub 2019 Dec 3.

Kearney Lab, Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland.

Porous collagen-glycosaminoglycan (collagen-GAG) scaffolds have shown promising clinical results for wound healing; however, these scaffolds do not replace the dermal and epidermal layer simultaneously and rely on local endogenous signaling to direct healing. Functionalizing collagen-GAG scaffolds with signaling factors, and/or additional matrix molecules, could help overcome these challenges. An ideal candidate for this is platelet-rich plasma (PRP) as it is a natural reservoir of growth factors, can be activated to form a fibrin gel, and is available intraoperatively. Read More

View Article and Full-Text PDF
December 2019

Comparison of two soft tissue substitutes for the treatment of gingival recession defects: an animal histological study.

J Appl Oral Sci 2019 7;27:e20180584. Epub 2019 Oct 7.

University of Michigan School of Dentistry, Department of Periodontics and Oral Medicine, Ann Arbor, MI, USA.

Objectives: This study aimed to compare two different soft tissue replacement grafts in their ability to treat gingival recession defects and successfully integrate with the surrounding tissues.

Methodology: Nine beagle dogs were included and followed up to 10 weeks. Sites for intervention were allocated to one of the grafting materials investigated. Read More

View Article and Full-Text PDF
October 2019

An iterative finite element-based method for solving inverse problems in traction force microscopy.

Comput Methods Programs Biomed 2019 Dec 30;182:105056. Epub 2019 Aug 30.

Aragón Institute of Engineering Research, Department of Mechanical Engineering, University of Zaragoza, Zaragoza, Spain.

Background And Objective: During the last years different model solutions were proposed for solving cell forces under different conditions. The solution relies on a deformation field that is obtained under cell relaxation with a chemical cocktail. Once the deformation field of the matrix is determined, cell forces can be computed by an inverse algorithm, given the mechanical properties of the matrix. Read More

View Article and Full-Text PDF
December 2019

Chitosan-collagen based film for controlled delivery of a combination of short life anesthetics.

Int J Biol Macromol 2019 Nov 28;140:1183-1193. Epub 2019 Aug 28.

Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 760 01 Zlin, Czech Republic.

The present research was undertaken to develop a chitosan-collagen film for controlled delivery of combinations of local anesthetics. The film has been prepared by casting which is a versatile, rapid and low-cost approach distinguished by high reproducibility. The mechanical, morphological, and physicochemical properties of the films and the impact of the drug loading were evaluated. Read More

View Article and Full-Text PDF
November 2019

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture.

ACS Appl Mater Interfaces 2019 Apr 12;11(16):14672-14683. Epub 2019 Apr 12.

Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, Pierre and Marie Curie Campus , 4 place Jussieu , 75252 Paris Cedex 05 , France.

Type I collagen is the main component of the extracellular matrix (ECM). In vitro, under a narrow window of physicochemical conditions, type I collagen self-assembles to form complex supramolecular architectures reminiscent of those found in native ECM. Presently, a major challenge in collagen-based biomaterials is to couple the delicate collagen fibrillogenesis events with a controlled shaping process in non-denaturating conditions. Read More

View Article and Full-Text PDF

Production and Characterization of Chemically Cross-Linked Collagen Scaffolds.

Methods Mol Biol 2019 ;1944:23-38

Regenerative, Modular and Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland.

Chemical cross-linking of collagen-based devices is used as a means of increasing the mechanical stability and control the degradation rate upon implantation. Herein, we describe techniques to produce cross-linked with glutaraldehyde (GTA; amine terminal cross-linker), 4-arm polyethylene glycol succinimidyl glutarate (4SP; amine terminal cross-linker), diphenyl phosphoryl azide (DPPA; carboxyl terminal cross-linker), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; carboxyl terminal cross-linker) collagen films. In addition, we provide protocols to characterize the biophysical (swelling), biomechanical (tensile), and biological (metabolic activity, proliferation and viability using human dermal fibroblasts and THP-1 macrophages) properties of the cross-linked collagen scaffolds. Read More

View Article and Full-Text PDF

Oral Collagen Supplementation: A Systematic Review of Dermatological Applications

J Drugs Dermatol 2019 01;18(1):9-16

Importance: The use of nutraceuticals such as collagen for skincare has been rising, but regulations are lacking on quality, absorption, and efficacy. To address this knowledge gap, clinical studies regarding the potential effects of collagen-based dietary supplements on skin are being completed. Objective: To review the literature and assess available randomized-controlled trials using collagen supplementation for treatment efficacy regarding skin quality, anti-aging benefits, and potential application in medical dermatology. Read More

View Article and Full-Text PDF
January 2019

Hyaluronan/collagen hydrogels containing sulfated hyaluronan improve wound healing by sustained release of heparin-binding EGF-like growth factor.

Acta Biomater 2019 03 17;86:135-147. Epub 2019 Jan 17.

Department of Dermatology, Venereology and Allergology, Leipzig University, Johannisallee 30, 04103 Leipzig, Germany. Electronic address:

Functional biomaterials that are able to bind, stabilize and release bioactive proteins in a defined manner are required for the controlled delivery of such to the desired place of action, stimulating wound healing in health-compromised patients. Glycosaminoglycans (GAG) represent a very promising group of components since they may be functionally engineered and are well tolerated by the recipient tissues due to their relative immunological inertness. Ligands of the Epidermal Growth Factor (EGF) receptor (EGFR) activate keratinocytes and dermal fibroblasts and, thus, contribute to skin wound healing. Read More

View Article and Full-Text PDF

Engineering the migration and attachment behaviour of primary dermal fibroblasts.

Biotechnol Bioeng 2019 05 28;116(5):1102-1115. Epub 2019 Jan 28.

Smart Matrix Limited, Mount Vernon Hospital, Northwood, UK.

The availability of primary cells present in pathological conditions is often very limited due to stringent ethical regulation and patient consent. One such condition is chronic wounds, where dermal fibroblasts show a deficient migration. In vitro models with cellular tools that mimic the in vivo scenario would be advantageous to test new therapies for these challenging wounds. Read More

View Article and Full-Text PDF