3,851 results match your criteria collagen fibril


Identification of potential genes related to breast cancer brain metastasis in breast cancer patients.

Biosci Rep 2021 Sep 20. Epub 2021 Sep 20.

Hebei University, Baoding City, China.

Brain metastases (BM) usually develop in breast cancer patients. Thus, the molecular mechanisms of breast cancer brain metastasis (BCBM) are of great importance in designing therapeutic strategies to treat or prevent BCBM. This study attempted to identify novel diagnostic and prognostic biomarkers of BCBM. Read More

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September 2021

Mechanical Stimulation Muscle Activity Is Necessary for the Maturation of Tendon Multiscale Mechanics During Embryonic Development.

Front Cell Dev Biol 2021 3;9:725563. Epub 2021 Sep 3.

Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, United States.

During embryonic development, tendons transform into a hypocellular tissue with robust tensile load-bearing capabilities. Previous work suggests that this mechanical transformation is due to increases in collagen fibril length and is dependent on mechanical stimulation muscle activity. However, the relationship between changes in the microscale tissue structure and changes in macroscale tendon mechanics is still unclear. Read More

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September 2021

Stability and remineralization of proteoglycan-infused dentin substrate.

Dent Mater 2021 Sep 15. Epub 2021 Sep 15.

Division of Comprehensive Oral Health, Adams School of Dentistry, The University of North Carolina at Chapel Hill, NC, USA. Electronic address:

Objective: This study tested the effects of small leucine-rich proteoglycan (SLRP) proteins on phosphoric acid (PA)-treated dentin bonding overtime and the role of such SLRPs in the remineralization potential of demineralized dentin collagen.

Methods: Coronal dentin sections of human molars were used. SLRPs were either decorin (DCN) or biglycan (BGN) in core or proteoglycan form (with glycosaminoglycans, GAGs). Read More

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September 2021

Collagen Fibril Orientation in Tissue Specimens from Atherosclerotic Plaque Explored Using Small Angle X-Ray Scattering.

J Biomech Eng 2021 Sep 16. Epub 2021 Sep 16.

Mechanical Engineering Dept., Stanford University, Stanford, CA 94305.

Atherosclerotic plaques can gradually develop in certain arteries. Disruption of fibrous tissue in plaques can result in plaque rupture and thromboembolism, leading to heart attacks and strokes. Collagen fibrils are important tissue building blocks and tissue strength depends on how fibrils are oriented. Read More

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September 2021

Identification and Integrated Analysis of circRNA and miRNA of Radiation-Induced Lung Injury in a Mouse Model.

J Inflamm Res 2021 2;14:4421-4431. Epub 2021 Sep 2.

Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China.

Background: Radiation-induced lung injury (RILI) is a main threat to patients who received thoracic radiotherapy. Thus, understanding the molecular mechanism of RILI is of great importance. Circular RNAs (circRNAs) have been found to act as a regulator of multiple biological processes, and the circRNA-microRNA (miRNA)-mRNA axis could play an important role in the signaling pathway of many human diseases including radiation injury. Read More

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September 2021

Declines in PDE4B activity promote myopia progression through downregulation of scleral collagen expression.

Exp Eye Res 2021 Sep 8;212:108758. Epub 2021 Sep 8.

School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China; State Key Laboratory of Optometry, Ophthalmology and Vision Science, Wenzhou, Zhejiang, China; Research Unit of Myopia Basic Research and Clinical Prevention and Control, Chinese Academy of Medical Sciences (2019RU025), Wenzhou, Zhejiang, China. Electronic address:

Myopia is the most common cause of a visual refractive error worldwide. Cyclic adenosine monophosphate (cAMP)-linked signaling pathways contribute to the regulation of myopia development, and increases in cAMP accumulation promote myopia progression. To pinpoint the underlying mechanisms by which cAMP modulates myopia progression, we performed scleral transcriptome sequencing analysis in form-deprived mice, a well-established model of myopia development. Read More

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September 2021

Design and Production of Customizable and Highly Aligned Fibrillar Collagen Scaffolds.

ACS Biomater Sci Eng 2021 Sep 10. Epub 2021 Sep 10.

Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States.

The ability to fabricate anisotropic collagenous materials rapidly and reproducibly has remained elusive despite decades of research. Balancing the natural propensity of monomeric collagen (COL) to spontaneously polymerize with the mild processing conditions needed to maintain its native substructure upon polymerization introduces challenges that are not easily amenable with off-the-shelf instrumentation. To overcome these challenges, we have designed a platform that simultaneously aligns type I COL fibrils under mild shear flow and builds up the material through layer-by-layer assembly. Read More

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September 2021

Mechanisms of reducing joint stiffness by blocking collagen fibrillogenesis in a rabbit model of posttraumatic arthrofibrosis.

PLoS One 2021 7;16(9):e0257147. Epub 2021 Sep 7.

Department of Orthopaedic Surgery, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America.

Posttraumatic fibrotic scarring is a significant medical problem that alters the proper functioning of injured tissues. Current methods to reduce posttraumatic fibrosis rely on anti-inflammatory and anti-proliferative agents with broad intracellular targets. As a result, their use is not fully effective and may cause unwanted side effects. Read More

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September 2021

The water soluble zinc based metal-organic frameworks (Zn-MOFs) as potential inhibitors for collagen fibrillogenesis.

Int J Biol Macromol 2021 Sep 1;190:56-60. Epub 2021 Sep 1.

Advanced Materials Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India. Electronic address:

Small molecules ranging from organic to inorganic systems have been reported as stabilizing agents for collagen. Various transition metal complexes have been utilized as tanning agent. However, as per the environmental norms issued by various regulatory agencies, the presence of certain metals such as Cr, Fe, Al, Zr and Ti in leather has been restricted to minimal amount (50 ppm), an unsurmountable task. Read More

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September 2021

Peracetic Acid-Ethanol Processed Human Tendon Allograft: A Morphological, Biochemical, and Biomechanical Study In Vitro.

Orthop Surg 2021 Sep 3. Epub 2021 Sep 3.

Department of Bone and Soft Tissue Oncology, Tianjin Hospital, Tianjin, China.

Objective: To clarify the morphological, biochemical, and biomechanical effects of peracetic acid-ethanol sterilization processing to human hamstring tendon allografts for different time periods.

Methods: Thirty-two fresh-frozen human hamstring tendon allografts obtained from an allograft supplier were prepared and incubated in peracetic acid-ethanol solution (PES) containing 1% v/v peracetic acid and 24% v/v ethanol. Specimens were randomly classified into four groups according to the PES processing time (untreated as the control group, 30 min as the PES30 group, 120 min as the PES120 group, and 240 min as the PES240group). Read More

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September 2021

Proteoglycan and collagen contribution to the strain-rate-dependent mechanical behaviour of knee and shoulder cartilage.

J Mech Behav Biomed Mater 2021 Aug 25;124:104733. Epub 2021 Aug 25.

School of Mechanical, Medical & Process Engineering, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia. Electronic address:

The contribution of the proteoglycan to the strain-rate-dependent mechanical behaviour of cartilage tissues has been suggested to decrease with an increase in the strain-rate. On the other hand, the contribution from the collagen network has been suggested to increase as the strain-rate increases. These conclusions are drawn mainly based on numerical studies conducted on high-load-bearing knee cartilage tissues, while experimental evidence of these behaviours have not been demonstrated previously. Read More

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Synthesis and Assembly of Recombinant Collagen.

Methods Mol Biol 2021 ;2347:83-96

School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.

Collagen represents the major structural protein of the extracellular matrix. The desired mechanical and biological performances of collagen that have led to its broad applications as a building block in a great deal of fields, such as tissue engineering, drug delivery, and nanodevices. The most direct way to obtain collagen is to separate and extract it from biological tissues, but these top-down methods are usually cumbersome, and the structure of collagen is usually destroyed during the preparation process. Read More

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January 2021

Collagen V insufficiency in a mouse model for Ehlers Danlos-syndrome affects viscoelastic biomechanical properties explaining thin and brittle corneas.

Sci Rep 2021 Aug 30;11(1):17362. Epub 2021 Aug 30.

Laboratory of Ocular Cell Biology, CABMM, University of Zurich, Zurich, Switzerland.

Ehlers-Danlos syndrome (EDS) is a genetic disease leading to abnormalities in mechanical properties of different tissues. Here we quantify corneal biomechanical properties in an adult classic EDS mouse model using two different measurement approaches suited for murine corneal mechanical characterization and relate differences to stromal structure using Second Harmonic Generation (SHG) microscopy. Quasi-static Optical Coherence Elastography (OCE) was conducted non-invasively during ambient pressure modulation by - 3 mmHg. Read More

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Functional and structural properties of human patellar articular cartilage in osteoarthritis.

J Biomech 2021 Sep 12;126:110634. Epub 2021 Jul 12.

Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.

Changes in the fibril-reinforced poroelastic (FRPE) mechanical material parameters of human patellar cartilage at different stages of osteoarthritis (OA) are not known. Further, the patellofemoral joint loading is thought to include more sliding and shear compared to other knee joint locations, thus, the relations between structural and functional changes may differ in OA. Thus, our aim was to determine the patellar cartilage FRPE properties followed by associating them with the structure and composition. Read More

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September 2021

Contrasting Local and Macroscopic Effects of Collagen Hydroxylation.

Int J Mol Sci 2021 Aug 23;22(16). Epub 2021 Aug 23.

Department of Chemical and Biological Engineering, Department of Physics, Illinois Institute of Technology, Chicago, IL 60616, USA.

Collagen is heavily hydroxylated. Experiments show that proline hydroxylation is important to triple helix (monomer) stability, fibril assembly, and interaction of fibrils with other molecules. Nevertheless, experiments also show that even without hydroxylation, type I collagen does assemble into its native D-banded fibrillar structure. Read More

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Distinct contributions of meprins to skin regeneration after injury - Meprin α a physiological processer of pro-collagen VII.

Matrix Biol Plus 2021 Aug 13;11:100065. Epub 2021 May 13.

Department of Dermatology, Faculty of Medicine and Medical Center - University of Freiburg, Germany.

Astacin-like proteinases (ALPs) are regulators of tissue and extracellular matrix (ECM) homeostasis. They convey this property through their ability to convert ECM protein pro-forms to functional mature proteins and by regulating the bioavailability of growth factors that stimulate ECM synthesis. The most studied ALPs in this context are the BMP-1/tolloid-like proteinases. Read More

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Procollagen C-proteinase enhancer-1 (PCPE-1), a potential biomarker and therapeutic target for fibrosis.

Matrix Biol Plus 2021 Aug 20;11:100062. Epub 2021 Apr 20.

University of Lyon, CNRS, Tissue Biology and Therapeutic Engineering Laboratory, LBTI, UMR5305, F-69367 Lyon, France.

The correct balance between collagen synthesis and degradation is essential for almost every aspect of life, from development to healthy aging, reproduction and wound healing. When this balance is compromised by external or internal stress signals, it very often leads to disease as is the case in fibrotic conditions. Fibrosis occurs in the context of defective tissue repair and is characterized by the excessive, aberrant and debilitating deposition of fibril-forming collagens. Read More

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Phase-Sensitive Vibrationally Resonant Sum-Frequency Generation Microscopy in Multiplex Configuration at 80 MHz Repetition Rate.

J Phys Chem B 2021 Aug 14;125(33):9507-9516. Epub 2021 Aug 14.

Department of Chemistry, University of California at Irvine, Irvine, California 92697-2025, United States.

Vibrationally resonant sum-frequency generation (VR SFG) microscopy is an advanced imaging technique that can map out the intensity contrast of infrared and Raman active vibrational modes with micron to submicron lateral resolution. To broaden its applications and to obtain a molecular level of understanding, further technical advancement is needed to enable high-speed measurements of VR SFG microspectra at every pixel. In this study, we demonstrate a new VR SFG hyperspectral imaging platform combined with an ultrafast laser system operated at a repetition rate of 80 MHz. Read More

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Anisotropy and inhomogeneity of permeability and fibrous network response in the pars intermedia of the human lateral meniscus.

Acta Biomater 2021 Aug 16. Epub 2021 Aug 16.

Complex Structure of Surgical Sciences and Technologies, IRCCS istituto Ortopedico Rizzoli, Bologna, Italy.

Within the human tibiofemoral joint, meniscus plays a key role due to its peculiar time-dependent mechanical characteristics, inhomogeneous structure and compositional features. To better understand the pathophysiological mechanisms underlying this essential component, it is mandatory to analyze in depth the relationship between its structure and the function it performs in the joint. Accordingly, the aim of this study was to evaluate the behavior of both solid and fluid phases of human meniscus in response to compressive loads, by integrating mechanical assessment and histological analysis. Read More

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New mechanistic insights to PLOD1-mediated human vascular disease.

Transl Res 2021 Aug 13. Epub 2021 Aug 13.

The Dorothy Davis Heart and Lung Research Institute and the Frick Center for Heart Failure and Arrhythmia, The Ohio State University, Columbus, Ohio; The Ohio State University College of Medicine and Wexner Medical Center, Division of Cardiovascular Medicine, Department of Internal Medicine, Columbus, Ohio. Electronic address:

Heritable thoracic aortic disease and familial thoracic aortic aneurysm/dissection are important causes of human morbidity/mortality, most without identifiable genetic cause. In a family with familial thoracic aortic aneurysm/dissection, we identified a missense p. (Ser178Arg) variant in PLOD1 segregating with disease, and evaluated PLOD1 enzymatic activity, collagen characteristics and in human aortic vascular smooth muscle cells, studied the effect on function. Read More

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Sequence-dependent mechanics of collagen reflect its structural and functional organization.

Biophys J 2021 Sep 12;120(18):4013-4028. Epub 2021 Aug 12.

Department of Molecular Biology and Biochemistry, Burnaby, British Columbia, Canada; Department of Physics, Burnaby, British Columbia, Canada; Department of Chemistry, Burnaby, British Columbia, Canada; Centre for Cell Biology, Development and Disease, Simon Fraser University, Burnaby, British Columbia, Canada. Electronic address:

Extracellular matrix mechanics influence diverse cellular functions, yet surprisingly little is known about the mechanical properties of their constituent collagen proteins. In particular, network-forming collagen IV, an integral component of basement membranes, has been far less studied than fibril-forming collagens. A key feature of collagen IV is the presence of interruptions in the triple-helix-defining (Gly-X-Y) sequence along its collagenous domain. Read More

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September 2021

Habitual side-specific loading leads to structural, mechanical and compositional changes in the patellar tendon of young and senior life-long male athletes.

J Appl Physiol (1985) 2021 08 12. Epub 2021 Aug 12.

Institute of Sports Medicine Copenhagen, Bispebjerg-Frederiksberg Hospitals, Denmark.

Effects of life-long physical activity on tendon function have been investigated in cross-sectional studies, but these are at risk of "survivorship" bias. Here, we investigate if life-long side-specific loading is associated with greater cross-sectional area (CSA), mechanical properties, cell density (DNA content) and collagen cross-link composition of the male human patellar tendon (PT), in vivo. Nine seniors and six young male life-long elite badminton players and fencers were included. Read More

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Collagen fibril assembly: New approaches to unanswered questions.

Matrix Biol Plus 2021 Dec 13;12:100079. Epub 2021 Jul 13.

Wellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.

Collagen fibrils are essential for metazoan life. They are the largest, most abundant, and most versatile protein polymers in animals, where they occur in the extracellular matrix to form the structural basis of tissues and organs. Collagen fibrils were first observed at the turn of the 20th century. Read More

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December 2021

A collagen-silica-based biocomposite for potential application in bone tissue engineering.

J Biomed Mater Res A 2021 Aug 10. Epub 2021 Aug 10.

Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Anatomía Patológica, Buenos Aires, Argentina.

Bone is a hierarchical material that has inspired the design of biopolymer-derived biocomposites for tissue engineering purposes. The present study sought to synthesize and perform the physicochemical characterization and biocompatibility of a collagen-silica-based biocomposite for potential application in bone tissue engineering. Ultrastructure, biodegradability, swelling behavior, and biocompatibility properties were analyzed to gain insight into the advantages and limitations to the use of this biomaterial as a bone substitute. Read More

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High glycosyltransferase 8 domain containing two protein levels contribute to poor prognosis in urothelial carcinoma.

Int J Urol 2021 Aug 9. Epub 2021 Aug 9.

Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.

Objective: To examine the expression levels of the glycosyltransferase 8 domain containing protein 2 and its clinical implications in urothelial carcinoma patients.

Methods: Data mining, immunohistochemistry together with H-score calculation was carried out to evaluate the glycosyltransferase 8 domain containing protein 2 levels on tissue specimens from urothelial carcinoma patients, retrospectively. Correlations between glycosyltransferase 8 domain containing protein 2 H-score and imperative clinicopathological factors were measured. Read More

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DEFICIENCY OF PROCOLLAGEN C-PROTEINASE ENHANCER 1 (PCPE1) IN MICE HAS NO MAJOR IMPACT ON CARDIAC COLLAGEN AND FUNCTION UNDER BASAL CONDITIONS.

J Cardiovasc Pharmacol 2021 Aug 5. Epub 2021 Aug 5.

Cardiovascular and Metabolic Diseases Research, Institut de Recherches Servier, Suresnes, France. Non-Clinical Safety, Biologie Servier, Gidy, France. Program of Cardiovascular Diseases, CIMA Universidad de Navarra, IdiSNA and CIBERCV, Pamplona, Spain. Departments of Cardiology and Cardiac Surgery and of Nephrology, Clínica Universidad de Navarra, Pamplona, Spain. Cardiovascular and Metabolic Diseases, Institut de Recherches Internationales Servier, Suresnes, France.

Abstract: Maturation of fibrillar collagen is known to play a crucial role in the pathophysiology of myocardial fibrosis. Procollagen C-Proteinase Enhancer 1 (PCPE1) has a key role in procollagen maturation and collagen fibril formation.The phenotype of both male and female PCPE1 knock-out mice was investigated under basal conditions to explore the potential of PCPE1 as a therapeutic target in heart failure. Read More

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A mouse model of brittle cornea syndrome caused by mutation in Zfp469.

Dis Model Mech 2021 Sep 22;14(9). Epub 2021 Sep 22.

MRC Human Genetics Unit, Institute of Genetics and Cancer, The University of Edinburgh, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.

Brittle cornea syndrome (BCS) is a rare recessive condition characterised by extreme thinning of the cornea and sclera. BCS results from loss-of-function mutations in the poorly understood genes ZNF469 or PRDM5. In order to determine the function of ZNF469 and to elucidate pathogenic mechanisms, we used genome editing to recapitulate a human ZNF469 BCS mutation in the orthologous mouse gene Zfp469. Read More

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September 2021

Hydrous titanium oxide and bayberry tannin co-immobilized nano collagen fibrils for uranium extraction from seawater and recovery from nuclear wastewater.

Chemosphere 2021 Jul 23;286(Pt 1):131626. Epub 2021 Jul 23.

State Key Laboratory of Environment-friendly Energy Materials, School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, PR China; Engineering Research Center of Biomass Materials, Ministry of Education, National Collaborative Innovation Center for Nuclear Waste Disposal and Environmental Safety, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, PR China. Electronic address:

Extraction uranium from complicated aqueous solutions (seawater and nuclear wastewater) has been promoting the development of multi-functional adsorbents with high adsorption capacities and high selectivity. Here, we proposed a co-immobilization approach to preparing uranium adsorbents. Due to specific recognition and binding between functional groups, bayberry tannin (BT) and hydrous titanium oxide (HTO) were co-immobilized onto nano collagen fibrils (NCFs). Read More

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Elastic, Dynamic Viscoelastic and Model-Derived Fibril-Reinforced Poroelastic Mechanical Properties of Normal and Osteoarthritic Human Femoral Condyle Cartilage.

Ann Biomed Eng 2021 Sep 2;49(9):2622-2634. Epub 2021 Aug 2.

Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland.

Osteoarthritis (OA) degrades articular cartilage and weakens its function. Modern fibril-reinforced poroelastic (FRPE) computational models can distinguish the mechanical properties of main cartilage constituents, namely collagen, proteoglycans, and fluid, thus, they can precisely characterize the complex mechanical behavior of the tissue. However, these properties are not known for human femoral condyle cartilage. Read More

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September 2021

The ultrastructure of cartilage tissue and its swelling response in relation to matrix health.

J Anat 2021 Aug 1. Epub 2021 Aug 1.

Department of Chemical and Materials Engineering, University of Auckland, Auckland, New Zealand.

This multi-length scale anatomical study explores the influence of mild cartilage structural degeneration on the tissue swelling response. While the swelling response of cartilage has been studied extensively, this is the first study to reveal and correlate tissue microstructure and ultrastructure, with the swelling induced cartilage tissue strains. Cartilage sample strips (n = 30) were obtained from the distal-lateral quadrant of thirty mildly degenerate bovine patellae and, following excision from the bone, the cartilage strips were allowed to swell freely for 2 h in solutions of physiological saline and distilled water successively. Read More

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