1,936 results match your criteria collagen denaturation

Myosin sensitivity to thermal denaturation explains differences in water loss and shrinkage during cooking in muscles of distinct fibre types.

Meat Sci 2021 Apr 10;179:108521. Epub 2021 Apr 10.

Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, Australia.

The effect of thermal protein denaturation on the structure and quality of muscles of different fibre types is not well understood. Unaged masseter (100% type I fibres) and cutaneous trunci (93% type II fibres) muscles (N = 10) were assessed for their characteristics, protein denaturation, cooking loss, Warner- Bratzler shear force (WBSF) and shrinkage after heating at 50 °C - 85 °C with a rate of 5 °C/ min. Raw masseter had a higher pH, collagen and water content, shorter sarcomere, comparable fibre diameter, and shorter and wider fragments upon homogenization, than cutaneous trunci. Read More

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Influence of Lipidation on the Folding and Stability of Collagen Triple Helices-An Experimental and Theoretical Study.

J Am Chem Soc 2021 Apr 8;143(15):5937-5942. Epub 2021 Apr 8.

Laboratory of Organic Chemistry, ETH Zurich, D-CHAB, Vladimir-Prelog-Weg 3, 8093 Zurich, Switzerland.

The folding of triple-helical collagen, the most abundant protein in nature, relies on the nucleation and propagation along the strands. Hydrophobic moieties are crucial for the folding and stability of numerous proteins. Instead, nature uses for collagen a trimerization domain and - prolyl isomerases to facilitate and accelerate triple helix formation. Read More

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Fabrication and Properties of Electrospun Collagen Tubular Scaffold Crosslinked by Physical and Chemical Treatments.

Polymers (Basel) 2021 Feb 28;13(5). Epub 2021 Feb 28.

Deptartment of Biobased Materials Science, Kyoto Institute of Technology, Kyoto 606-8585, Japan.

Tissue engineered scaffold was regarded as a promising approach instead of the autograft. In this study, small diameter electrospun collagen tubular scaffold with random continuous smooth nanofibers was successfully fabricated. However, the dissolution of collagen in concentrated aqueous (conc. Read More

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February 2021

Evaluation of a novel nano-size collagenous matrix film cross-linked with gallotannins catalyzed by laccase.

Food Chem 2021 Jul 20;351:129335. Epub 2021 Feb 20.

College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address:

The effect of hydrolysis degree of gallotannins (GT, 1 mg/g) on cross-linking of nano-size collagen catalyzed by laccase (12 U/g) was studied, and the antibacterial properties of GT hydrolysates (HGT)-laccase (Lac) collagen films on minced cod were also investigated. The results showed that the tensile strength of HGT-Lac films (87.23-100. Read More

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Effect of Heat Level and Expose Time on Denaturation of Collagen Tissues.

Cell Mol Bioeng 2021 Feb 28;14(1):113-119. Epub 2020 Sep 28.

Biomedical Engineering Department, School of Engineering and Natural Sciences, Istanbul Medeniyet University, 34700 Istanbul, Turkey.

Introduction: The applied heat level and expose time are main issues in certain operations/applications, such as a laser assisted tissue welding, preparation of collagen-based biomaterials (films, implants). Therefore, the precise investigation of these parameters is crucial. The results can serve as a guideline to assess potential effects while maintaining the functionality of the collagen structures. Read More

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February 2021

Binding of collagen gene products with titanium oxide.

J Biochem 2021 Feb 25. Epub 2021 Feb 25.

Faculty of Environmental Earth Science, Hokkaido University, Sapporo Japan 060-0810.

Titanium is the only metal to which osteoblasts can adhere and on which they can grow and form bone tissue in vivo, resulting in a strong bond between the implant and living bone. This discovery provides the basis for the universal medical application of Ti. However, the biochemical mechanism of bond formation is still unknown. Read More

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February 2021

Proteomic fingerprints of damage in extracellular matrix assemblies.

Matrix Biol Plus 2020 Feb 30;5:100027. Epub 2020 Jan 30.

Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK.

In contrast to the dynamic intracellular environment, structural extracellular matrix (ECM) proteins with half-lives measured in decades, are susceptible to accumulating damage. Whilst conventional approaches such as histology, immunohistochemistry and mass spectrometry are able to identify age- and disease-related changes in protein abundance or distribution, these techniques are poorly suited to characterising molecular damage. We have previously shown that mass spectrometry can detect tissue-specific differences in the proteolytic susceptibility of protein regions within fibrillin-1 and collagen VI alpha-3. Read More

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February 2020

Characterization of Collagen from Sakhalin Taimen Skin as Useful Biomass.

Food Technol Biotechnol 2020 Dec;58(4):445-454

Nagoya Research Institute, Toyoake, 4701131 Aichi, Japan.

Research Background: Animal collagen has been widely utilized in foods, cosmetics and biomedical fields. The non-edible portion, such as fish skin and bones, are obtained during cooking. Most of them are currently discarded as wastes, although the nutritional value of the skin and bones is high. Read More

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December 2020

The influence of animal species, gender and tissue on the structural, biophysical, biochemical and biological properties of collagen sponges.

J Mater Sci Mater Med 2021 Jan 21;32(1):12. Epub 2021 Jan 21.

Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.

Although collagen type I is extensively used in biomedicine, no study to-date has assessed how the properties of the produced scaffolds are affected as a function of species, gender and tissue from which the collagen was extracted. Herein, we extracted and characterised collagen from porcine and bovine, male and female and skin and tendon tissues and we subsequently fabricated and assessed the structural, biophysical, biochemical and biological properties of collagen sponges. All collagen preparations were of similar purity and free-amine content (p > 0. Read More

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January 2021

Sutureless energy-based wound closure: a step in the quest for trocar site hernia prevention.

Minim Invasive Ther Allied Technol 2021 Jan 17:1-6. Epub 2021 Jan 17.

Department of General Surgery, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.

Objective: Easy and safe methods of fascia closure are needed in order to reduce the risk for trocar site hernias without affecting procedure time significantly. Here we present a method for port site closure using heat induced collagen denaturation.

Material And Methods: Controlled heat-induced collagen denaturation was applied to laparoscopic trocar sites in living porcine animal models. Read More

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January 2021

A new method for determining the denaturation temperature of collagen.

Food Chem 2021 May 16;343:128393. Epub 2020 Oct 16.

Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China; College of Marine Life and Fisheries, Jiangsu Ocean University, Lianyungang, China. Electronic address:

The denaturation temperature of collagen has been determined using several methods, such as circular dichroism, fourier transform infrared spectroscopy, and differential scanning calorimetry, etc. Such methods need specific equipment or mass samples (more than 75 mg samples), which means higher inspection costs. In this study, Sirius red was employed to determine the Tds of collagen from calf tendon, silver carp skin, frog and salmon skins (38. Read More

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Extraction and Characterization of Collagen from Elasmobranch Byproducts for Potential Biomaterial Use.

Mar Drugs 2020 Dec 4;18(12). Epub 2020 Dec 4.

3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal.

With the worldwide increase of fisheries, fish wastes have had a similar increase, alternatively they can be seen as a source of novel substances for the improvement of society's wellbeing. Elasmobranchs are a subclass fished in high amounts, with some species being mainly bycatch. They possess an endoskeleton composed mainly by cartilage, from which chondroitin sulfate is currently obtained. Read More

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December 2020

Modeling early thermal injury using an ex vivo human skin model of contact burns.

Burns 2020 Aug 29. Epub 2020 Aug 29.

Department of Surgery, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA. Electronic address:

Background: Early mechanisms underlying the progressive tissue death and the regenerative capability of burn wounds are understudied in human skin. A clinically relevant, reproducible model for human burn wound healing is needed to elucidate the early changes in the human burn wound environment. This study reports a reproducible contact burn model on human skin that explores the extent of tissue injury and healing over time, and defines the inter-individual variability in human skin to enable use in mechanistic studies on burn wound progression and healing. Read More

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Relationship between meat quality and intramuscular collagen characteristics of muscles from calf-fed, yearling-fed and mature crossbred beef cattle.

Meat Sci 2021 Mar 19;173:108375. Epub 2020 Nov 19.

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. Electronic address:

Intramuscular Ehrlich Chromogen (EC) and pyridinoline (Pyr) concentrations in the gluteus medius (GM) and semitendinosus (ST) from crossbred Angus calf- (n = 14) and yearling-fed (n = 14) steer and mature cow (MC, n = 12) carcasses were related to collagen and intramuscular connective tissue (IMCT) thermal stability and peak Warner-Bratzler shear force (WBSF). In both muscles, Pyr density was greater in MC, while EC concentrations were comparable in calf- and yearling-fed steer muscles and lowest in MC muscles. Thermal denaturation temperature and enthalpy of IMCT were highest in both muscles when from MC, although only total collagen was correlated with WBSF in calf fed-yearling fed steer data. Read More

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Vein wall shrinkage induced by thermal coagulation with high-intensity-focused ultrasound: numerical modeling and experiments in sheep.

Int J Hyperthermia 2020 ;37(1):1238-1247

Physics for Medicine Paris, Inserm, ESPCI Paris, CNRS, PSL Research University, Paris, France.

Background: Varicose veins are a common disease that may significantly affect quality of life. Different approaches are currently used in clinical practice to treat this pathology.

Materials And Methods: In thermal therapy (radiofrequency or laser therapy), the vein is directly heated to a high temperature to induce vein wall coagulation, and the heat induces denaturation of the intramural collagen, which results macroscopically in vein shrinkage. Read More

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January 2020

Effect of magnesium ascorbyl phosphate on collagen stabilization for wound healing application.

Int J Biol Macromol 2021 Jan 26;166:333-341. Epub 2020 Oct 26.

Biological Materials Laboratory, CSIR - Central Leather Research Institute, Adyar, Chennai 600 020, India. Electronic address:

Wound healing is a complex process which requires appropriate structural support for restoration of tissue continuity and function. Collagen can act as a template for cellular activities but poor physico-chemical properties necessitates the stabilization of collagen without impairing its structure and function. This study investigates the effect of magnesium ascorbyl phosphate (MAP) on collagen with reference to physico-chemical properties. Read More

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January 2021

Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution.

Materials (Basel) 2020 Oct 22;13(21). Epub 2020 Oct 22.

Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.

Collagen, thanks to its biocompatibility, biodegradability and weak antigenicity, is widely used in dressings and scaffolds, also as electrospun fibers. Its mechanical stability can be improved by adding polycaprolactone (PCL), a synthetic and biodegradable aliphatic polyester. While previously collagen/PCL combinations were electrospun in solvents such as hexafluoroisopropanol (HFIP) or trifluoroethanol (TFE), more recently literature describes collagen/PCL nanofibers obtained in acidic aqueous solutions. Read More

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October 2020

Type II Collagen from Cartilage of Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin.

Mar Drugs 2020 Oct 11;18(10). Epub 2020 Oct 11.

Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan.

Type II collagen is an important component of cartilage; however, little is known about its effect on skin wound healing. In this study, type II collagen was extracted from the cartilage of and its effect on in vitro and in vivo wound healing was compared to type I collagen derived from tilapia skin. Sturgeon cartilage collagen (SCC) was composed of α1 chains and with a thermal denaturation (T) at 22. Read More

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October 2020

The Influence of UV Light on Rheological Properties of Collagen Extracted from Silver Carp Skin.

Materials (Basel) 2020 Oct 8;13(19). Epub 2020 Oct 8.

Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarin 7 street, 87-100 Torun, Poland.

Acid soluble collagen (ASC) was extracted from fish skin. Collagen was dissolved in acetic acid at varying concentrations and its rheological properties were studied. Steady shear flow properties of collagen solutions at concentrations of 5 and 10 mg/mL were characterized using rheometry at 20 °C. Read More

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October 2020

Collagen denaturation is initiated upon tissue yield in both positional and energy-storing tendons.

Acta Biomater 2020 12 6;118:153-160. Epub 2020 Oct 6.

Department of Biomedical Engineering, University of Utah, United States; Scientific Computing and Imaging Institute, University of Utah, United States; Department of Orthopaedics, University of Utah, United States. Electronic address:

Tendons are collagenous soft tissues that transmit loads between muscles and bones. Depending on their anatomical function, tendons are classified as positional or energy-storing with differing biomechanical and biochemical properties. We recently demonstrated that during monotonic stretch of positional tendons, permanent denatured collagen begins accumulating upon departing the linear region of the stress-strain curve. Read More

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December 2020

Highly specific recognition of denatured collagen by fluorescent peptide probes with the repetitive Gly-Pro-Pro and Gly-Hyp-Hyp sequences.

J Mater Chem B 2020 11;8(44):10093-10100

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.

Denatured collagen is a key biomarker for various critical diseases such as cancer. Peptide probes with the repetitive (Gly-Pro-Hyp)n sequences have recently been found to selectively target denatured collagen; however, thermal or UV pretreatment is required to drive the peptides into the monomer conformation, which poses a substantial challenge for clinical applications. We herein construct two peptide probes, FAM-GOO and FAM-GPP, consisting of the repetitive (Gly-Hyp-Hyp)8 and (Gly-Pro-Pro)8 sequences, respectively. Read More

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November 2020

Accumulation of collagen molecular unfolding is the mechanism of cyclic fatigue damage and failure in collagenous tissues.

Sci Adv 2020 Aug 28;6(35):eaba2795. Epub 2020 Aug 28.

Department of Biomedical Engineering, University of Utah, Salt Lake City, UT 84112, USA.

Overuse injuries to dense collagenous tissues are common, but their etiology is poorly understood. The predominant hypothesis that micro-damage accumulation exceeds the rate of biological repair is missing a mechanistic explanation. Here, we used collagen hybridizing peptides to measure collagen molecular damage during tendon cyclic fatigue loading and computational simulations to identify potential explanations for our findings. Read More

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Use of collagen and auricular cartilage in bioengineering: scaffolds for tissue regeneration.

Cell Tissue Bank 2020 Sep 2. Epub 2020 Sep 2.

Interunit Graduate Program in Bioengineering, University of São Paulo, São Carlos, SP, Brazil.

The aim of this study was the development of collagen and collagen/auricular cartilage scaffolds for application in dermal regeneration. Collagen was obtained from bovine tendon by a 72 h-long treatment, while bovine auricular cartilage was treated for 24 h and divided into two parts, external (perichondrium, E) and internal (elastic cartilage, I). The scaffolds were prepared by mixing collagen (C) with the internal part (CI) or the external part (CE) in a 3:1 ratio. Read More

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September 2020

In situ structural studies during denaturation of natural and synthetically crosslinked collagen using synchrotron SAXS.

J Synchrotron Radiat 2020 Sep 19;27(Pt 5):1376-1381. Epub 2020 Aug 19.

Leather and Shoe Research Association of New Zealand, PO Box 8094, Palmerston North 4446, New Zealand.

Collagen is an important biomacromolecule, making up the majority of the extracellular matrix in animal tissues. Naturally occurring crosslinks in collagen stabilize its intermolecular structure in vivo, whereas chemical treatments for introducing synthetic crosslinks are often carried out ex vivo to improve the physical properties or heat stability of the collagen fibres for applications in biomaterials or leather production. Effective protection of intrinsic natural crosslinks as well as allowing them to contribute to collagen stability together with synthetic crosslinks can reduce the need for chemical treatments. Read More

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September 2020

The effect of various pre-treatment methods of chromium leather shavings in continuous biogas production.

Eng Life Sci 2020 Mar 24;20(3-4):79-89. Epub 2019 Nov 24.

Forschungsinstitut für Leder und Kunststoffbahnen (FILK) gGmbH Freiberg Germany.

During leather manufacture, high amounts of chromium shavings, wet by-products of the leather industry, are produced worldwide. They are stable towards temperatures of up to 110°C and enzymatic degradation, preventing anaerobic digestion in a biogas plant. Hitherto, chromium shavings are not utilized industrially to produce biogas. Read More

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Thermal stable characteristics of acid- and pepsin-soluble collagens from the carapace tissue of Chinese soft-shelled turtle (Pelodiscus sinensis).

Tissue Cell 2020 Dec 6;67:101424. Epub 2020 Aug 6.

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China. Electronic address:

The carapace from the Chinese soft-shelled turtle (Pelodiscus sinensis) is used as a traditional Chinese medicine. Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from turtle carapace were isolated and characterized to screen novel collagen material in this study. Yields of 1. Read More

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December 2020

Exploring the potential of TEM analysis for understanding cooking at prehistoric feasting sites.

Sci Rep 2020 08 12;10(1):13635. Epub 2020 Aug 12.

School of History, Archaeology, and Religion, Cardiff University, Colum Drive, Cardiff, CF10 3EU, UK.

This study explores the utility of transmission electron microscopy (TEM) analysis of bone collagen for investigating prehistoric cooking. Approaches to cooking practices have relied principally on artefactual evidence, macroscopic bone modification, and organic residue analysis. However, direct evidence for cooking of bone has been limited. Read More

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Optical imaging of collagen fiber damage to assess thermally injured human skin.

Wound Repair Regen 2020 11 21;28(6):848-855. Epub 2020 Aug 21.

Department of Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Surgery is the definitive treatment for burn patients who sustain full-thickness burn injuries. Visual assessment of burn depth is made by the clinician early after injury but is accurate only up to 70% of the time among experienced surgeons. Collagen undergoes denaturation as a result of thermal injury; however, the association of collagen denaturation and cellular death in response to thermal injury is unknown. Read More

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November 2020

Comparison of acid-soluble collagen characteristic from three important freshwater fish skins in Mekong Delta Region, Vietnam.

J Food Biochem 2020 09 26;44(9):e13397. Epub 2020 Jul 26.

Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.

The utilization of three important freshwater fish skins including tra catfish (Pangasianodon hypophthalmus), clown knifefish (Chitala ornata), and tilapia (Oreochromis niloticus) in Mekong Delta Region, Vietnam for acid-soluble collagen (ASC) extraction was investigated. From the SDS-PAGE profile, ASCs can be identified as type I collagen (consisting of two different α- chains and β component in protein pattern).The solubility among individual collagens was highest at pH 1-2, depending on fish species. Read More

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September 2020

Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles.

Materials (Basel) 2020 Jul 13;13(14). Epub 2020 Jul 13.

National Laboratory of Nanotechnology (LANOTEC), National Center of High Technology (LANOTEC-CeNAT-CONARE), Pavas, 1174-1200 San José, Costa Rica.

Propolis natural extracts have been used since ancient times due to their antioxidant, anti-inflammatory, antiviral, and antimicrobial activities. In this study, we produced scaffolds of type I collagen, extracted from Wistar Hanover rat tail tendons, and impregnated them with propolis nanoparticles (NPs) for applications in regenerative medicine. Our results show that the impregnation of propolis NPs to collagen scaffolds affected the collagen denaturation temperature and tensile strength. Read More

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