130,202 results match your criteria coenzymes

Glucose-oxidase like catalytic mechanism of noble metal nanozymes.

Nat Commun 2021 06 7;12(1):3375. Epub 2021 Jun 7.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, PR China.

Au nanoparticles (NPs) have been found to be excellent glucose oxidase mimics, while the catalytic processes have rarely been studied. Here, we reveal that the process of glucose oxidation catalyzed by Au NPs is as the same as that of natural glucose oxidase, namely, a two-step reaction including the dehydrogenation of glucose and the subsequent reduction of O to HO by two electrons. Pt, Pd, Ru, Rh, and Ir NPs can also catalyze the dehydrogenation of glucose, except that O is preferably reduced to HO. Read More

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sp. nov. isolated from refrigerated dry-aged beef.

Int J Syst Evol Microbiol 2021 Jun;71(6)

Department of Safety and Quality of Meat, Max Rubner-Institut, Federal Research Institute for Nutrition and Food, E.-C.-Baumann Straße 20, D-95326 Kulmbach, Germany.

A polyphasic approach was applied to investigate the diversity of microbiota that evolved during cold storage beef ripening. Isolate V4/DAB/S4/2a with a unique BOX-rep-PCR fingerprint profile revealed more than 99 % nucleotide identities upon pairwise comparisons of 16S rDNA sequences from the type strains DSM 101070, DSM 108989, DSM 26521 and DSM 29166, placing it within the / branch of the genus . Additional based comparison revealed DSM 101070 as the nearest relative, with 98. Read More

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Screening of biotin-binding F-antibodies from autodisplayed F-library on E. coli outer membrane.

Anal Chim Acta 2021 Jul 10;1169:338627. Epub 2021 May 10.

Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, South Korea. Electronic address:

This study aimed to isolate F-antibodies with biotin-binding activity from a F-antibody library that was successfully screened on the outer membrane of E. coli. The aims were achieved by (1) preparing a library of F-antibodies on the outer membrane of E. Read More

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Pseudomyxoma retroperitonei masquerading as a psoas abscess.

BMJ Case Rep 2021 Jun 2;14(6). Epub 2021 Jun 2.

Radiology, Seth GS Medical College and KEM Hospital, Mumbai, Maharashtra, India

A 52-year-old woman was referred to our department with multiple discharging sinuses and swelling in the right flank and iliac region for the past year. Ultrasound revealed a large collection in the right psoas muscle with the sinus tract reaching up to the skin in the right iliac region. Despite repeated attempts at drainage, the collection continued to increase in size. Read More

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Flavin-dependent halogenases catalyze enantioselective olefin halocyclization.

Nat Commun 2021 06 1;12(1):3268. Epub 2021 Jun 1.

Department of Chemistry, Indiana University, Bloomington, IN, 47405, USA.

Halocyclization of alkenes is a powerful bond-forming tool in synthetic organic chemistry and a key step in natural product biosynthesis, but catalyzing halocyclization with high enantioselectivity remains a challenging task. Identifying suitable enzymes that catalyze enantioselective halocyclization of simple olefins would therefore have significant synthetic value. Flavin-dependent halogenases (FDHs) catalyze halogenation of arene and enol(ate) substrates. Read More

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Pyrroloquinoline quinone-dependent glucose dehydrogenase bioelectrodes based on one-step electrochemical entrapment over single-wall carbon nanotubes.

Talanta 2021 Sep 16;232:122386. Epub 2021 Apr 16.

Departamento de Química Física and Instituto Universitario de Materiales de Alicante (IUMA), University of Alicante, Ap. 99, 03080, Alicante, Spain. Electronic address:

Development of effective direct electron transfer is considered an interesting platform to obtain high performance bioelectrodes. Therefore, designing of scalable and cost-effective immobilization routes that promotes correct direct electrical contacting between the electrode material and the redox enzyme is still required. As we present here, electrochemical entrapment of pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) on single-wall carbon nanotube (SWCNT)-modified electrodes was carried out in a single step during electrooxidation of para-aminophenyl phosphonic acid (4-APPA) to obtain active bioelectrodes. Read More

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September 2021

Bioenergetic Alterations of Metabolic Redox Coenzymes as NADH, FAD and FMN by Means of Fluorescence Lifetime Imaging Techniques.

Int J Mol Sci 2021 May 31;22(11). Epub 2021 May 31.

Core Facility Confocal and Multiphoton Microscopy, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.

Metabolic FLIM (fluorescence lifetime imaging) is used to image bioenergetic status in cells and tissue. Whereas an attribution of the fluorescence lifetime of coenzymes as an indicator for cell metabolism is mainly accepted, it is debated whether this is valid for the redox state of cells. In this regard, an innovative algorithm using the lifetime characteristics of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) to calculate the fluorescence lifetime induced redox ratio (FLIRR) has been reported so far. Read More

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Increased Cuticle Waxes by Overexpression of WSD1 Improves Osmotic Stress Tolerance in and .

Int J Mol Sci 2021 May 13;22(10). Epub 2021 May 13.

Stockbridge School of Agriculture, University of Massachusetts Amherst, Amherst, MA 01003, USA.

To ensure global food security under the changing climate, there is a strong need for developing 'climate resilient crops' that can thrive and produce better yields under extreme environmental conditions such as drought, salinity, and high temperature. To enhance plant productivity under the adverse conditions, we constitutively overexpressed a bifunctional () gene, which plays a critical role in wax ester synthesis in Arabidopsis stem and leaf tissues. The qRT-PCR analysis showed a strong upregulation of transcripts by mannitol, NaCl, and abscisic acid (ABA) treatments, particularly in shoots. Read More

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Ubiquinone Metabolism and Transcription HIF-1 Targets Pathway Are Toxicity Signature Pathways Present in Extracellular Vesicles of Paraquat-Exposed Human Brain Microvascular Endothelial Cells.

Int J Mol Sci 2021 May 11;22(10). Epub 2021 May 11.

Department of Medicine, University of Geneva, 1206 Geneva, Switzerland.

Over the last decade, the knowledge in extracellular vesicles (EVs) biogenesis and modulation has increasingly grown. As their content reflects the physiological state of their donor cells, these "intercellular messengers" progressively became a potential source of biomarker reflecting the host cell state. However, little is known about EVs released from the human brain microvascular endothelial cells (HBMECs). Read More

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Tissue Imaging and Quantification Relying on Endogenous Contrast.

Adv Exp Med Biol 2021 ;3233:257-288

Department of Biomedical Engineering, Tufts University, Medford, MA, USA.

Cell-matrix interactions play an important role in regulating a variety of essential processes in multicellular organisms, and are closely associated with numerous diseases. Modified interactions have major effects upon key features of both cells and extracellular matrix (ECM), and a thorough understanding of changes in these features can lead to critically important insights of diseases as well as the identification of effective therapeutic targets. Here, we summarize recent advances in quantitative, optical imaging of cellular metabolism and ECM spatial organization using endogenous sources of contrast. Read More

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The evolutionary stages of the complexity of biological catalysts mark and clarify the phases of the origin of the genetic code: A model for the origin of the reading frame with codons from proto-mRNAs with different frames.

Biosystems 2021 May 27:104449. Epub 2021 May 27.

The Ionian School, Genetic Code and tRNA Origin Laboratory, Via Roma 19, 67030, Alfedena, L'Aquila, Italy; Institute of Biosciences and Bioresources, National Research Council, Via P. Castellino, 111, 80131, Naples, Italy. Electronic address:

I analyse the origin of the genetic code in the light of the evolution of biological catalysts. I discuss the rudimentary forms that the genetic code assumed in the presence of a catalysis performed by ions or by low molecular weight molecules, such as nucleotide coenzymes. However, it is only with the advent of a mixed polymer made of RNA and peptides - covalently linked - that the genetic code took on a clearer form. Read More

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[Cloning and functional verification of carboxyl CoA ligases(AeCCLs) in Arnebia euchroma].

Zhongguo Zhong Yao Za Zhi 2021 May;46(9):2182-2189

Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

Carboxyl CoA ligases(CCLs) is an important branch of adenylate synthetase gene family, which mainly has two-step catalytic reactions. Firstly, in the presence of adenosine triphosphate, it can catalyze the pyrophosphorylation of carboxylateswith diffe-rent structures to form corresponding acyl adenosine monophosphate intermediates. Secondly, adenosine monophosphate was replaced by free electrons in the mercaptan group of enzyme A or other acyl receptors by nucleophilic attack to form thioesters. Read More

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Crystal Structure Analysis of Cationic Peroxidase from Proso Millet and Identification of Its Phosphatase Active Sites.

J Agric Food Chem 2021 Jun 28;69(22):6251-6259. Epub 2021 May 28.

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan030006, China.

Proso millet peroxidase (PmPOD) belongs to class III plant peroxidases, which are enzymes typically characterized by their heme coenzymes. PmPOD exhibits not only heme-dependent peroxidase activity but also heme-independent phosphatase activity. Crystal structure analysis and sequence alignment showed that PmPOD contained a phosphatase catalytic loop CXXXXXR in its β-domain that is similar to the active site of a dual-specific phosphatase. Read More

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Effects of alpha-lipoic acid treatment on serum progranulin levels and inflammatory markers in diabetic neuropathy.

J Int Med Res 2021 May;49(5):3000605211012213

Department of Internal Medicine, University of Debrecen Faculty of Medicine, Debrecen, Hungary.

Objectives: Progranulin (PGRN) is a secreted growth factor that helps to regulate neuronal survival by blocking tumor necrosis factor-alpha (TNFα) receptors. The antioxidant alpha-lipoic acid (ALA) is used in diabetic neuropathy to improve nerve conduction and relieve neuropathic pain, but its effects on PGRN levels have not yet been elucidated.

Methods: In this prospective study, 54 patients with type 2 diabetes and peripheral neuropathy received 600 mg of ALA daily for 6 months. Read More

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Metastatic pancreatic cancer with complete response to FOLFIRINOX treatment.

BMJ Case Rep 2021 May 24;14(5). Epub 2021 May 24.

Oncology, Tom Baker Cancer Centre, Calgary, Alberta, Canada

A 59-year-old woman presented with abdominal pain associated with nausea and night sweats. A large mass was found in the pancreatic tail and innumerable liver lesions were identified. Ultrasound-guided biopsy of a liver nodule confirmed moderately differentiated adenocarcinoma consistent with a pancreatobiliary primary. Read More

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[The forensic implications of the relationship between the proteolytic enzymes activity and the changes in NADH and FAD fluorescence intensity in skeletal muscle when determining the time of death (experimental study)].

Sud Med Ekspert 2021 ;64(3):24-28

Medical Institute of the Russian Peoples Friendship University, Moscow, Russia.

Objective: Evaluation of the relationship between the activity of proteolytic enzymes (cathepsin D and calpains) and the dynamics of the fluorescence intensity of the coenzymes nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) in the rats' skeletal muscles in relation to the time of death. The proteolytic activity of enzymes in rat skeletal muscle was determined at the postmortem time points corresponding to the most significant changes in the dynamics of coenzymes NADH and FAD fluorescence intensity. The proteolytic enzymes activity was found to be low during the period of increasing intensity of NADH fluorescence observed within 3 hours after death. Read More

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The effect of corneal crosslinking on the rigidity of the cornea estimated using a modified algorithm for the Schiøtz tonometer.

Indian J Ophthalmol 2021 06;69(6):1531-1536

Kyiv City Clinical Ophthalmological Hospital "Eye Microsurgical Center", Kyiv, Ukraine.

Purpose: The aim of this study was to test a method for estimating corneal rigidity before and after cross-linking (CXL) using a Schiøtz tonometer.

Methods: The study was performed in the Kyiv City Clinical Ophthalmological Hospital "Eye Microsurgical Center", Ukraine. This was a prospective, consecutive, randomized, masked, case-by-case, clinical study. Read More

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sp. nov., isolated from marine sediment.

Int J Syst Evol Microbiol 2021 May;71(5)

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, PR China.

A Gram-stain negative, rod-shaped, facultatively aerobic, pale-beige-coloured bacterial strain, designated F7233, was isolated from coastal sediment sampled at Jingzi Bay, Weihai, PR China. Cells of strain F7233 were 0.3-0. Read More

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gen. nov., sp. nov. isolated from Red Sea mangrove sediments belongs to the recently proposed family within the order Rhizobiales.

Int J Syst Evol Microbiol 2021 May;71(5)

Biological and Environmental Sciences and Engineering Division (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.

We isolated a novel strain, R1DC25, described as gen. nov. sp. Read More

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sp. nov., a cellulolytic bacterium isolated from agricultural soil.

Int J Syst Evol Microbiol 2021 May;71(5)

Research Group for Food Biotechnology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary.

A novel Gram-reaction-negative bacterial strain, designated Ka43, was isolated from agricultural soil and characterised using a polyphasic approach to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, the strain shows highest similarity (97.1 %) to E50. Read More

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sp. nov, associated with leaf spot disease of .

Int J Syst Evol Microbiol 2021 May;71(5)

Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.

Two Gram-negative, aerobic, motile bacteria strains were isolated from leaf spot disease of . Strain hsmgli-8 has 99.86 % 16S rRNA gene sequence similarity to LY10J, and the highest 16S rRNA gene sequence similarity to 58 (97. Read More

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Cell-specific transcriptional control of mitochondrial metabolism by TIF1γ drives erythropoiesis.

Science 2021 05;372(6543):716-721

Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 01238, USA.

Transcription and metabolism both influence cell function, but dedicated transcriptional control of metabolic pathways that regulate cell fate has rarely been defined. We discovered, using a chemical suppressor screen, that inhibition of the pyrimidine biosynthesis enzyme dihydroorotate dehydrogenase (DHODH) rescues erythroid differentiation in bloodless zebrafish mutant embryos defective for transcriptional intermediary factor 1 gamma (). This rescue depends on the functional link of DHODH to mitochondrial respiration. Read More

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Rapid determination of folic acid and riboflavin in urine by polypyrrole magnetic solid-phase extractant combined ultra-performance liquid chromatography.

J Chromatogr A 2021 Jul 27;1648:462192. Epub 2021 Apr 27.

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

Determination of folic acid and riboflavin in biological samples is difficult due to their high polarity, low concentration, chemical instability, and complex matrix. In this study, the polypyrrole-coated magnetic nanocomposite ([email protected]) was synthesized innovatively with the assistance of hexadecyltrimethylammonium bromide. To evaluate the adsorption mechanism and the feasibility of synthesized [email protected] as an adsorbent, the adsorption capacities, kinetics and thermodynamics of folic acid and riboflavin were investigated systemically. Read More

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Lipid Profile and Vascular Remodelling in Young Dyslipidemic Subjects Treated with Nutraceuticals Derived from Red Yeast Rice.

Cardiovasc Ther 2021 22;2021:5546800. Epub 2021 Apr 22.

Department of Medicine (DIMED), University of Padova, Italy.

Background And Aims: A relevant role is emerging for functional foods in cardiovascular prevention. The aim of this study was to assess the effect of a nutraceutical multitargeted approach on lipid profile and inflammatory markers along with vascular remodelling in a cohort of dyslipidemic subjects without history of cardiovascular (CV) disease.

Methods And Results: We enrolled 25 subjects (mean age 48. Read More

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Nicotinamide riboside attenuates age-associated metabolic and functional changes in hematopoietic stem cells.

Nat Commun 2021 05 11;12(1):2665. Epub 2021 May 11.

Biomedical Manufacturing Commonwealth Scientific and Industrial Research Organisation (CSIRO), Melbourne, VIC, Australia.

With age, hematopoietic stem cells (HSC) undergo changes in function, including reduced regenerative potential and loss of quiescence, which is accompanied by a significant expansion of the stem cell pool that can lead to haematological disorders. Elevated metabolic activity has been implicated in driving the HSC ageing phenotype. Here we show that nicotinamide riboside (NR), a form of vitamin B3, restores youthful metabolic capacity by modifying mitochondrial function in multiple ways including reduced expression of nuclear encoded metabolic pathway genes, damping of mitochondrial stress and a decrease in mitochondrial mass and network-size. Read More

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[Subarachnoid Hemorrhage Associated with Bevacizumab during Chemotherapy for Clinical Stage Ⅳ Sigmoid Colon Cancer-A Case Report].

Gan To Kagaku Ryoho 2021 Apr;48(4):596-598

Dept. of Gastroenterological Surgery, Toho University Omori Medical Center.

The case is a 59‒year‒old woman. A detailed examination of fecal occult blood revealed a diagnosis of cStage Ⅳ sigmoid colon cancer cT3N1M1b(liver H2, hilar liver and celiac artery lymph nodes). After excision of the primary lesion, decided to give chemotherapy. Read More

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[A Case of Liver Metastasis of Colorectal Cancer in a Hemodialysis Patient, Underwent Liver Resection and Chemotherapy after Colorectal Resection].

Gan To Kagaku Ryoho 2021 Apr;48(4):552-554

Dept. of Surgery, Ako City Hospital.

A 60 years old man has undergone hemodialysis due to chronic renal failure by diabetic nephropathy. A sigmoid colon cancer and multiple liver metastases(S2, S6)were found, and he had laparoscopic sigmoid colon resection first, it was local resection. The stage was pT3N2aH1, stage Ⅳ, and histopathological examination revealed that the tumor was well‒differentiated adenocarcinoma and RAS, BRAF mutation was negative. Read More

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Optical Redox Imaging Differentiates Triple-Negative Breast Cancer Subtypes.

Adv Exp Med Biol 2021 ;1269:253-258

Britton Chance Laboratory of Redox Imaging, Department of Radiology and Johnson Research Foundation, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Triple-negative breast cancer (TNBC) is a highly diverse group of cancers with limited treatment options, responsible for about 15% of all breast cancers. TNBC cells differ from each other in many ways such as gene expression, metabolic activity, tumorigenicity, and invasiveness. Recently, many research and clinical efforts have focused on metabolically targeted therapy for TNBC. Read More

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Potential Biomarker for Triple-Negative Breast Cancer Invasiveness by Optical Redox Imaging.

Adv Exp Med Biol 2021 ;1269:247-251

Britton Chance Laboratory of Redox Imaging, Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Predicting tumor metastatic potential remains a challenge in cancer research and in clinical diagnosis. Cancer invasion to neighboring tissues is a significant event in cancer progression to metastasis. Optical redox imaging (ORI) is based on detecting the endogenous fluorescence signals of reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavin adenine dinucleotide (FAD). Read More

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Unraveling the mechanism of efficient adsorption of riboflavin onto activated biochar derived from algal blooms.

J Environ Manage 2021 Aug 5;291:112725. Epub 2021 May 5.

School of Geographic Sciences, Nantong University, Nantong, 226007, China. Electronic address:

Riboflavin is commercially produced primarily by bio-fermentation. Nonetheless, purification and separation are particularly complex and costly. Adsorption from the fermentation liquor is an alternative riboflavin separation technology during which a cost-efficient adsorbent is highly desired. Read More

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