10,240 results match your criteria clostridium perfringens

Antimicrobial effects of plant extracts against Clostridium perfringens with respect to food-relevant influencing factors.

J Food Prot 2021 Jun 11. Epub 2021 Jun 11.

LMU München: Ludwig-Maximilians-Universitat Munchen Schoenleutner Strasse 8 GERMANY Oberschleissheim 85764.

The application of plant extracts (PEs) could be a promising option to satisfy consumers' demand for natural additives to inhibit growth of variable pathogenic bacteria. Thus, the aim of this study was to develop a standardized microdilution method to examine the antimicrobial effects of ten hydrophilic plant extracts against two strains of C. perfringens facing various food-relevant influencing factors. Read More

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Mixtures of natural antimicrobials can reduce Campylobacter jejuni, Salmonella enterica and Clostridium perfringens infections and cellular inflammatory response in MDCK cells.

Gut Pathog 2021 Jun 7;13(1):37. Epub 2021 Jun 7.

Food Microbiology, Bacteriology Branch, Veterinary Sciences Division, Agri-Food and Biosciences Institute, 18a Newforge Lane, Belfast, BT9 5PX, Northern Ireland, UK.

Background: The classification of natural antimicrobials as potential antibiotic replacements is still hampered by the absence of clear biological mechanisms behind their mode of action. This study investigated the mechanisms underlying the anti-bacterial effect of a mixture of natural antimicrobials (maltodextrin, citric acid, sodium citrate, malic acid, citrus extract and olive extract) against Campylobacter jejuni RC039, Salmonella enterica SE 10/72 and Clostridium perfringens ATCC® 13124 invasion of Madin-Darby Canine Kidney cells (MDCK).

Results: Minimum sub-inhibitory concentrations were determined for Campylobacter jejuni (0. Read More

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Using surrogate data to assess risks associated with microbial peak events in source water at drinking water treatment plants.

Water Res 2021 May 25;200:117296. Epub 2021 May 25.

Canada Research Chair in Source Water Protection, Department of Civil, Geological, and Mining Engineering, Polytechnique Montreal, Montreal, Quebec, H3C 3A7, Canada.

A monitoring strategy was implemented at two drinking water treatment plants in Quebec, Canada, to evaluate microbial reduction performances of full-scale treatment processes under different source water conditions. β-D-glucuronidase activity in source water was automatically monitored in near-real-time to establish baseline and event conditions at each location. High-volume water samples (50-1,500 L) were collected at the inflow and the outflow of coagulation/flocculation, filtration, and UV disinfection processes and were analysed for two naturally occurring surrogate organisms: Escherichia coli and Clostridium perfringens. Read More

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Densitometric Analysis of rep-PCR Data: Insight into Genetic Variability and Transmission of Clostridium perfringens Typed with an Improved Multiplex PCR.

Anaerobe 2021 Jun 2:102383. Epub 2021 Jun 2.

Department of Biotechnology, Gauhati University, Assam, India, Pin 781014. Electronic address:

An epidemiological study was conducted in North-East India (part of Indo-Burma biodiversity hotspot) to better understand the distribution, diversity, and transmission of Clostridium perfringens among livestock, pets, wild animals (captive), and humans. A total of 160 C. perfringens isolates were recovered from 642 diarrhoeic faecal samples with an isolation rate of 24. Read More

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Research Note: First report on the detection of necrotic enteritis (NE) B-like toxin in biological samples from NE-afflicted chickens using capture enzyme-linked immunosorbent assay.

Poult Sci 2021 Apr 20;100(7):101190. Epub 2021 Apr 20.

Southern Poultry Research Group, Inc., 1061 Hale Road, Watkinsville, GA 30677, USA.

Necrotic enteritis (NE) is a devastating enteric disease caused by Clostridium perfringens type G. One of the pore-forming toxins, NE B-like (NetB) toxin, secreted by pathogenic C. perfringens type G, has been proposed to be the main virulent factor in NE pathogenesis. Read More

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Immunostimulatory Potential of Fruits and Their Extracts in Poultry.

Front Immunol 2021 17;12:641696. Epub 2021 May 17.

Guelph Research and Development Center, Agriculture and Agri-Food Canada, Guelph, ON, Canada.

The impact of antibiotic use for growth promotion in livestock and poultry production on the rise of antimicrobial resistance (AMR) in bacteria led to the ban of this practice in the European Union in 2006 and a restriction of antimicrobial use (AMU) in animal agriculture in Canada and the United States of America. There is a high risk of infectious diseases such as necrotic enteritis due to , and colibacillosis due to avian pathogenic in antimicrobial-free broiler chickens. Thus, efficient and cost-effective methods for reducing AMU, maintaining good poultry health and reducing public health risks (food safety) are urgently needed for poultry production. Read More

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Large Clostridial Toxins: Mechanisms and Roles in Disease.

Microbiol Mol Biol Rev 2021 Jun 2:e0006421. Epub 2021 Jun 2.

Molecular Medicine Program, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada.

Large clostridial toxins (LCTs) are a family of bacterial exotoxins that infiltrate and destroy target cells. Members of the LCT family include Clostridioides difficile toxins TcdA and TcdB, Paeniclostridium sordellii toxins TcsL and TcsH, Clostridium novyi toxin TcnA, and Clostridium perfringens toxin TpeL. Since the 19th century, LCT-secreting bacteria have been isolated from the blood, organs, and wounds of diseased individuals, and LCTs have been implicated as the primary virulence factors in a variety of infections, including C. Read More

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Ca and Mg Concentrations in Spices and Growth of Commonly Sporulated and Non-Sporulated Food-Borne Microorganisms According to Marketing Systems.

Foods 2021 May 19;10(5). Epub 2021 May 19.

Department of Nutrition and Bromatology, School of Pharmacy, University of Granada, 18071 Granada, Spain.

Ca and Mg levels were determined in five spices according to marketing system (in bulk or commercialized in glass or polyethylene terephthalate (PET) containers) and correlated with microbial growth of commonly sporulated ( and ) and non-sporulated (, psychrophilic and mesophilic bacteria, and yeasts and molds) food-borne pathogens present in them, when they were previously added to the microbial culture media. The basil had the highest mean Ca and Mg level and showed the highest microbial growth in the food-borne pathogenic microorganisms studied ( < 0.001). Read More

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Monoglyceride Blend Reduces Mortality, Improves Nutrient Digestibility, and Intestinal Health in Broilers Subjected to Clinical Necrotic Enteritis Challenge.

Animals (Basel) 2021 May 17;11(5). Epub 2021 May 17.

School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.

This study evaluated the potential of monoglyceride blend (MG) and buffered formic acid (FA) as alternatives to antibiotics in the performance and intestinal health of broilers under clinical necrotic enteritis (NE) challenge. A total of 544 as-hatched Ross 308 broiler chicks were randomly distributed to 32-floor pens housing 17 birds per pen. The four treatments were: NC-non-additive control; ZBS-antibiotic group supplemented with zinc bacitracin and salinomycin; MG-additive MG supplementation in the starter phase only; and MGFA-additive MG in starter phase and FA in grower and finisher phases. Read More

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Effective Microorganisms (EM) Improve Internal Organ Morphology, Intestinal Morphometry and Serum Biochemical Activity in Japanese Quails under Challenge.

Molecules 2021 May 8;26(9). Epub 2021 May 8.

Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 13, 10-719 Olsztyn, Poland.

The effect of effective microorganisms (EM) on internal organ morphology, intestinal morphometry, and serum biochemical activity in Japanese quails under challenge was determined. After 30 days of EM addition, one group of quails was orally inoculated with . The second group did not receive EM and was inoculated with . Read More

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Fermented Rapeseed Meal as a Component of the Mink Diet () Modulating the Gastrointestinal Tract Microbiota.

Animals (Basel) 2021 May 8;11(5). Epub 2021 May 8.

InventionBio, Wojska Polskiego 65, 85-825 Bydgoszcz, Poland.

Fermented rapeseed meal (FRSM) was used in the diet of American mink (). An advantage of this product is its prebiotic and functional properties, which can modify the bacterial microbiota of the GIT. A control group and three experimental groups were formed, with 60 animals in each group. Read More

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Protein C Gene Mutation in an Older Adult Patient with Septicemia-Related Visceral Vein Thrombosis.

TH Open 2021 Apr 26;5(2):e171-e173. Epub 2021 May 26.

Department of Laboratory Medicine, Uji-Tokushukai Medical Center, Uji, Japan.

A 78-year-old Japanese male with septicemia and cholecystitis was found to have thrombosis in the left branch of intrahepatic portal vein as well as superior mesenteric vein. Visceral vein thrombosis (VVT) in this case was associated with protein C deficiency, due to a heterozygous mutation, p. Arg185Met. Read More

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Predictive model for growth of Clostridium perfringens during cooling of cooked pork supplemented with sodium chloride and sodium pyrophosphate.

Meat Sci 2021 Oct 14;180:108557. Epub 2021 May 14.

Department of Food Science & Technology, University of Georgia, Athens, GA 30602, United States of America.

A dynamic model was developed to predict growth of Clostridium perfringens in cooked ground pork supplemented with salt (0-3% wt/wt) and sodium pyrophosphate (0-0.3% wt/wt) under varying temperatures. C. Read More

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October 2021

Agricultural Trauma causing Open Fractures: Is Antibiotic Coverage against Anaerobic Organisms Indicated?

J Orthop Trauma 2021 May 26. Epub 2021 May 26.

University of Iowa Hospital & Clinics, Department of Orthopedics & Rehabilitation, Iowa City, IA University of Mississippi Medical Center, Department of Orthopedic Surgery & Rehabilitation, Jackson, MS.

Objective: To compare deep infection rate and causative organisms in open fractures of the lower extremity from agricultural trauma to similar injuries in non-agricultural trauma.

Design: Retrospective.

Setting: Two tertiary-care institutions. Read More

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Enteric organisms detected in feces of dogs with bloody diarrhea: 45 cases.

Top Companion Anim Med 2021 May 24:100549. Epub 2021 May 24.

Veterinary School. Universidade Federal de Minas Gerais (UFMG). Av. Antônio Carlos, 6627. Belo Horizonte, MG, Brazil, CEP 31.270-901. Electronic address:

Bloody diarrhea is a common condition in dogs, but studies evaluating the enteropathogens involved specifically in adult dogs are scarce. In the present study, stool samples from 45 adult dogs with bloody diarrhea were evaluated for the four enteric organisms mainly reported in these cases: canine parvovirus type 2 (CPV-2), Clostridioides difficile, Clostridium perfringens, and Salmonella spp. In addition, the samples were also tested for coronavirus, rotavirus, Giardia spp. Read More

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Antimicrobial susceptibility profiles of invasive isolates of anaerobic bacteria from a large Canadian reference laboratory: 2012-2019.

Anaerobe 2021 May 25;70:102386. Epub 2021 May 25.

Department of Laboratory Medicine and Pathobiology, University of Toronto, Medical Sciences Building Room 6231, 1 King's College Circle, Toronto, Ontario, M5S 1A8, Canada; Public Health Ontario, 661 University Avenue, Suite 1701, Toronto, Ontario, M5G 1M1, Canada. Electronic address:

Anaerobic bacteria can cause severe and life threatening infections. Susceptibility data are relatively limited on anaerobic organisms despite the clinical importance in guiding empiric treatment of infections. To determine antimicrobial susceptibility profiles of clinically significant anaerobic bacteria, isolates obtained from sterile sites submitted to Public Health Ontario Laboratory (2012-2019) were included in this study (N = 5712). Read More

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Necrotizing soft tissue infection: clinical characteristics, diagnosis, and management of 32 cases in Beijing.

J Int Med Res 2021 May;49(5):3000605211018442

Department of Orthopedic Trauma, Peking University Fourth School of Clinical Medicine, Beijing Jishuitan Hospital, Beijing, China.

Objective: Necrotizing soft tissue infection (NSTI) is a rare but life-threatening bacterial infection. This study was performed to analyze the clinical characteristics of patients with NSTI admitted to the intensive care unit of a trauma and burn center in Beijing and to summarize the treatment experience.

Methods: This retrospective study involved patients with NSTI admitted to the intensive care unit from January 2010 to January 2020. Read More

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Septic Shock and Spontaneous Gangrenous Gas Necrosis of the Spleen Secondary to : The Importance of Source Control.

Case Rep Crit Care 2021 6;2021:5563071. Epub 2021 May 6.

Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, Emory University School of Medicine, 615 Michael Street, Suite 205, Atlanta, GA 30322, USA.

is a rare cause of septic shock, occurring most frequently in immunocompromised patients. An uncommon cause of septicemia is spontaneous gangrenous gas necrosis of the spleen, where the primary treatment is splenectomy. We present a case of septic shock caused by spontaneous gangrenous gas necrosis of the spleen secondary to in a patient whose profound pancytopenia made obtaining definitive source control extremely difficult. Read More

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Necrotic enteritis challenge regulates peroxisome proliferator-1 activated receptors signaling and β-oxidation pathways in broiler chickens.

Anim Nutr 2021 Mar 17;7(1):239-251. Epub 2020 Dec 17.

School of Environment and Rural Science, University of New England, Armidale, NSW, 2351, Australia.

Necrotic enteritis (NE) is an important enteric disease in poultry and has become a major concern in poultry production in the post-antibiotic era. The infection with NE can damage the intestinal mucosa of the birds leading to impaired health and, thus, productivity. To gain a better understanding of how NE impacts the gut function of infected broilers, global mRNA sequencing (RNA-seq) was performed in the jejunum tissue of NE challenged and non-challenged broilers to identify the pathways and genes affected by this disease. Read More

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CECT 5940 improves performance and gut function in broilers fed different levels of protein and/or under necrotic enteritis challenge.

Anim Nutr 2021 Mar 23;7(1):185-197. Epub 2020 Aug 23.

School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.

Two studies were conducted to investigate the effect of CECT 5940 (BA) as a probiotic on growth performance, amino acid digestibility and bacteria population in broiler chickens under a subclinical necrotic enteritis (NE) challenge and/or fed diets with different levels of crude protein (CP). Both studies consisted of a 2 × 2 factorial arrangement of treatments with 480 Ross 308 mix-sexed broiler chickens. In study 1, treatments included 1) NE challenge (+/-), and 2) BA (1. Read More

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Over-processed meat and bone meal and phytase effects on broilers challenged with subclinical necrotic enteritis: Part 3. Bone mineralization and litter quality.

Anim Nutr 2021 Mar 4;7(1):142-151. Epub 2021 Jan 4.

School of Environmental and Rural Science, University of New England, Armidale, NSW, 2351, Australia.

This study was conducted to determine the effect of necrotic enteritis (NE), phytase level and meat and bone meal (MBM) processing on bone mineralization of broilers and litter quality. Ross 308 male broiler chicks ( = 768) were allotted to 48 pens with 16 birds each. There were 8 dietary treatments in a 2 × 2 × 2 factorial arrangement. Read More

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Protein Truncating Variants of in Type G Strains.

Front Cell Infect Microbiol 2021 29;11:645248. Epub 2021 Apr 29.

Livestock Gut Health Team Ghent, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

Extracellular matrix (ECM) degrading enzymes produced by may play an important role during the initial phases of avian necrotic enteritis by facilitating toxin entry in the intestinal mucosa and destruction of the tissue.  is known to produce several ECM-degrading proteases, such as kappa toxin, an extracellular collagenase that is encoded by the gene. In this study, the  gene sequence of a collection of 48 strains, including pathogenic (i. Read More

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Clostridium Perfringens Septic Arthritis of the Sternoclavicular Joint.

J Emerg Med 2021 May 12. Epub 2021 May 12.

Department of Emergency Medicine, University Hospital Waterford, Waterford, Republic of Ireland.

Background: Clostridium perfringens septic arthritis of the sternoclavicular joint has not been reported previously.

Case Report: We present the case of a 70-year-old patient with a history of stage IV colon cancer who presented to the emergency department with chest and neck pain for 3 days. After assessment, he was discharged home on analgesics. Read More

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Inhibition of the interaction between fibronectin and dermatopontin by Clostridium perfringens fibronectin-binding proteins.

Microbiol Immunol 2021 May 15. Epub 2021 May 15.

Department of Life Science, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama-shi, Okayama, 700-0005, Japan.

Fibronectin (Fn) is an approximately 450 kDa glycoprotein that is comprised of 12 type I, 2 type II, and 15-17 type III modules. Fibrillation of Fn is important for tissue reconstitution and wound healing. We previously reported that Clostridium perfringens produces several Fn-binding proteins (Fbps), two of which, FbpA and FbpB, bind to III -C (a fragment of Fn derived from the carboxyl-terminal two-thirds of the first type III module). Read More

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MALDI-TOF Mass Spectrometry and 16S rRNA Gene Sequence Analysis for the Identification of Foodborne Clostridium Spp.

J AOAC Int 2021 May 12. Epub 2021 May 12.

U.S. Food and Drug Administration, Microbiological Sciences Branch, Southeast Food and Feed Laboratory, Office of Regulatory Science, Office of Regulatory Affairs, 60 8th Street NE, Atlanta, GA, 30309, USA.

Background: Clostridium is a genus of Gram-positive, spore-forming, anaerobic bacteria comprising approximately 100 species. Some Clostridium spp. (C. Read More

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Effects of miR-204 on apoptosis and inflammatory response of Clostridium perfringens beta2 toxin induced IPEC-J2 cells via targeting BCL2L2.

Microb Pathog 2021 Jul 11;156:104906. Epub 2021 May 11.

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China; Gansu Research Center for Swine Production Engineering and Technology, Lanzhou, 730070, China. Electronic address:

Clostridium perfringens beta2 (CPB2) toxin can cause intestinal damage and inflammatory responses in a variety of animals, which seriously endanger the healthy development of animal husbandry. Increasing evidence has demonstrated that microRNAs (miRNAs) can play an important regulatory role in the process of pathogenic infection. In our previous study, we found that miR-204 was highly expressed in the ileum tissues of the susceptible group diarrhea piglets after infection with Clostridium perfringens (C. Read More

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Epsilon toxin from Clostridium perfringens induces toxic effects on skin tissues and HaCaT and human epidermal keratinocytes.

Toxicon 2021 Jul 7;198:102-110. Epub 2021 May 7.

State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, PR China. Electronic address:

Epsilon toxin (ETX) is a key pathogenic factor of C. perfringens type B and D, causing fatal enterotoxemia in sheep and goats. Excessive production of ETX increases intestinal permeability; its entrance into the bloodstream leads to severe edema in organs such as the brain and kidneys. Read More

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Evaluation of different antigenic preparations against necrotic enteritis in broiler birds using a novel Clostridium perfringens type G strain.

Anaerobe 2021 May 3;70:102377. Epub 2021 May 3.

Department of Microbiology, College of Veterinary Science, AAU., Khanapara, Guwahati, Assam, India. Electronic address:

Objective: Keeping in view, the constraints faced by the Indian broiler industry with lack of a suitable vaccine against Necrotic Enteritis (NE), a study has been proposed to explore the prevalence and detail characterization of C. perfringens type G in NE suspected broiler chicken in the process of suitable vaccine development.

Methods: Intestinal scrapings/faecal contents of NE suspected broiler chickens were screened to establish the prevalence of C. Read More

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The MAL Protein, an Integral Component of Specialized Membranes, in Normal Cells and Cancer.

Cells 2021 Apr 30;10(5). Epub 2021 Apr 30.

Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.

The gene encodes a 17-kDa protein containing four putative transmembrane segments whose expression is restricted to human T cells, polarized epithelial cells and myelin-forming cells. The MAL protein has two unusual biochemical features. First, it has lipid-like properties that qualify it as a member of the group of proteolipid proteins. Read More

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Phenotypic detection and genotyping of associated with enterotoxemia in sheep in the Qassim Region of Saudi Arabia.

Vet World 2021 Mar 6;14(3):578-584. Epub 2021 Mar 6.

Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia.

Background And Aim: Enterotoxemia caused by toxinotypes is an often fatal disease of sheep of all ages, with a substantial economic loss to the sheep industry. This study was conducted to isolate from suspected cases of enterotoxemia in sheep in the central part of the Qassim Region, Saudi Arabia, and to determine the prevalent toxinotype by detecting alpha (), beta (), and epsilon () toxin genes, which might help control this disease locally.

Materials And Methods: A total of 93 rectal swabs and intestinal content samples were collected from diseased and animals suspected of having died of enterotoxemia in early 2020. Read More

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