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Baseline Characteristics and Survival of an Australian Interstitial Pneumonia with Autoimmune Features Cohort.

Respiration 2021 Apr 19:1-12. Epub 2021 Apr 19.

Department of Respiratory, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.

Background And Objective: The research term "interstitial pneumonia with autoimmune features" (IPAF) encompasses interstitial lung disease (ILD) patients with autoimmune features not meeting diagnostic criteria for a defined connective tissue disease (CTD). It remains unclear if IPAF is a distinct disease entity with implications for management and prognosis. We describe an Australian IPAF population and compare their baseline characteristics and outcomes with distinct cohorts of idiopathic interstitial pneumonia (IIP), CTD-ILD, and unclassifiable ILD. Read More

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Hydrogel from acellular porcine adipose tissue promotes survival of adipose tissue transplantation.

Biomed Mater 2021 Apr 19. Epub 2021 Apr 19.

Department of Breast Surgery, Clinical Research Center for Breast, Sichuan University West China Hospital, No.37 Guoxuexiang, Chengdu, Sichuan, China, Chengdu, Sichuan, 610041, CHINA.

Lipofilling is a popular technique for soft tissue augmentation, limited by unpredictable graft survival. This study aimed at exploring the effect of hydrogel from acellular porcine adipose tissue (HAPA) on angiogenesis and survival of adipose tissue used for lipofilling. The effect of HAPA on adipose-derived stem cells (ADSCs) proliferation, adipogenic differentiation, and vascular endothelial growth factor (VEGF) secretion were evaluated in hypoxia and normoxia in vitro. Read More

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The role of carbohydrate antigen 125 in COVID-19.

Authors:
Esra Dökümcü

Med Hypotheses 2021 Mar 29;151:110590. Epub 2021 Mar 29.

Medical Biochemistry Laboratory, Edirne Public Health Institution, Turkey. Electronic address:

Coronavirus disease 2019 (COVID-19) is an inflammatory process with complex pathophysiology and by affecting the cardiovascular system directly or indirectly that causes life threatening cardiac injuries. Therefore, clarifying the effects of this infection on the cardiovascular system is of importance in terms of the clinical course of the disease. The increases in cardiac and inflammatory biomarkers in COVID-19 have been associated with poor prognosis and mortality. Read More

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Anti-seizure medication correlated changes of cortical morphology in childhood epilepsy with centrotemporal spikes.

Epilepsy Res 2021 Mar 23;173:106621. Epub 2021 Mar 23.

Department of Medical Imaging, Jinling Hospital, The First School of Clinical Medicine, Southern Medical University, Nanjing, 210002, China; Department of Medical Imaging, Jinling Hospital, Nanjing University School of Medicine, Nanjing, 210002, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, 210093, China; Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, 149 Thirteenth Street, Suite 2301, Charlestown, MA, 02129, USA. Electronic address:

To investigate the morphological changes of cerebral cortex correlating with anti-seizure medication in Childhood Epilepsy with Centrotemporal Spikes (CECTS), and their relationships with seizure control. This study included a total of 188 children, including 62 patients with CECTS taking anti-seizure drugs, 56 patients with drug-naive, and 70 healthy controls. A portion of cases were also followed-up for longitudinal analysis. Read More

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Identification and application of a neutralizing epitope within alpha-hemolysin using human serum antibodies elicited by vaccination.

Mol Immunol 2021 Apr 16;135:45-52. Epub 2021 Apr 16.

National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing, 400038, PR China. Electronic address:

Staphylococcus aureus (SA), especially the methicillin-resistant variant (MRSA), is becoming a serious threat to human health in hospitals and communities, making the development of an effective vaccine urgent. Alpha-hemolysin (Hla) is a key virulence factor and also a good target for the development of SA vaccines. However, the epitopes in Hla recognized by human immunity are not characterized in detail, which hinders the design of epitope-based human vaccines against SA. Read More

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Obstructed hemivagina and ipsilateral renal anomaly (OHVIRA): Early diagnosis, treatment and outcomes.

Eur J Obstet Gynecol Reprod Biol 2021 Apr 16;261:12-16. Epub 2021 Apr 16.

Department of Urology, Children's Medicine Affiliated to Chongqing Medical University, Chongqing, PR China; Paediatrics Research Institute, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Centre for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, PR China; Chongqing Key Laboratory of Paediatrics, Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, PR China. Electronic address:

Objective: To describe and summarize the early diagnosis, treatment and outcomes of obstructed hemivagina and ipsilateral renal anomaly (OHVIRA).

Methods: Data from patients with OHVIRA treated at the study centre over the last decade were analysed retrospectively, including demographic characteristics, symptoms, diagnosis, treatment and outcomes. Relevant published literature was also reviewed in this study. Read More

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Clinical features and prognostic factors of pediatric idiopathic sudden hearing loss from moderate to profound degree.

Am J Otolaryngol 2021 Apr 16;42(5):103027. Epub 2021 Apr 16.

ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai 200031, China; NHC Key Laboratory of Hearing Medicine, Fudan University, Shanghai 200031, China. Electronic address:

Background: The pediatric idiopathic sudden sensorineural hearing loss (PISSNHL) is not rare in the clinics, however, the prognostic factors of PISSNHL are still unclear.

Objectives: To investigate the clinical and audiologic characteristics associated with prognosis in PISSNHL.

Material And Methods: Clinical and audiological characteristics and possible prognostic factors were retrospectively evaluated in 76 PISSNHL patients aged less than 19 years. Read More

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Data sharing in PredRet for accurate prediction of retention time: Application to plant food bioactive compounds.

Food Chem 2021 Apr 9;357:129757. Epub 2021 Apr 9.

Université Clermont Auvergne, INRAE, UNH, F-63000 Clermont Ferrand, France.

Prediction of retention times (RTs) is increasingly considered in untargeted metabolomics to complement MS/MS matching for annotation of unidentified peaks. We tested the performance of PredRet (http://predret.org/) to predict RTs for plant food bioactive metabolites in a data sharing initiative containing entry sets of 29-103 compounds (totalling 467 compounds, >30 families) across 24 chromatographic systems (CSs). Read More

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Targeting Cul3-scaffold E3 ligase complex via KLHL substrate adaptors for cancer therapy.

Pharmacol Res 2021 Apr 16:105616. Epub 2021 Apr 16.

Institute of Pharmacology and Toxicology, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China; Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, China; Cancer Center of Zhejiang University, Hangzhou, 310058, China. Electronic address:

Targeted therapy has become increasingly important and indispensable in cancer therapy. Cullin3-RING ligases (CRL3) serve as essential executors for regulating protein homeostasis in cancer development, highlighting that CRL3 might be promising targets in various cancer treatments. However, how to design new targeted therapies by disrupting the function of CRL3 are poorly understood. Read More

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The intervertebral disc from embryonic development to disc degeneration: insights into spatial cellular organization.

Spine J 2021 Apr 16. Epub 2021 Apr 16.

Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, D-72072 Tübingen, Germany; Department of Orthopaedic Surgery, University Hospital of Tübingen, D-72076 Tübingen, Germany. Electronic address:

Background Context: Low back pain is commonly attributed to intervertebral disc (IVD) degeneration. IVD resembles articular cartilage in its biochemical and cellular composition in many ways. For articular cartilage, degeneration stage-specific characteristic spatial chondrocyte patterns have recently been described. Read More

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Recombinase polymerase amplification with lateral flow strip for detecting Babesia microti infections.

Parasitol Int 2021 Apr 16:102351. Epub 2021 Apr 16.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Animal Epidemical Disease and Infectious Zoonoses, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei 430070, China. Electronic address:

Babesia microti is one of the most important pathogens causing humans and rodents babesiosis-an emerging tick-borne disease that occurs worldwide. At present, the gold standard for the detection of Babesia is the microscopic examination of blood smears, but this diagnostic test has several limitations. The recombinase polymerase amplification with lateral flow (LF-RPA) assay targeting the mitochondrial cytochrome oxidase subunit I (cox I) gene of B. Read More

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PET/CT helps to determine treatment duration in patients with resected as well as inoperable alveolar echinococcosis.

Parasitol Int 2021 Apr 16:102356. Epub 2021 Apr 16.

Department of Nuclear Medicine, University Hospital Zurich, University of Zurich, Switzerland.

Purpose: The aim of the study was to determine the role of F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) at the end of benzimidazole therapy in alveolar echinococcosis.

Methods: A total of 22 patients undergoing PET/CT at the end of benzimidazole therapy were retrospectively registered. Maximum standardized uptake values (SUV) were measured in remaining echinococcus manifestations and compared to normal liver tissue. Read More

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Genotyping atypical porcine pestivirus using NS5a.

Infect Genet Evol 2021 Apr 16:104866. Epub 2021 Apr 16.

Veterinary Diagnostic Laboratory and Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL, USA. Electronic address:

Atypical porcine pestivirus (APPV) is an emerging virus discovered in 2014 and it can cause congenital tremors in pigs. Molecular epidemiology serves as an essential tool in monitoring and controlling the disease. Virus epidemiology mainly relies on genome sequencing and phylogenetic characterization. Read More

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A GDF5 frameshift mutation segregating with Grebe type chondrodysplasia and Brachydactyly Type C+ in a 6 generations family: Clinical report and mini review.

Eur J Med Genet 2021 Apr 16:104226. Epub 2021 Apr 16.

Human Molecular Genetics Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE) College, PIEAS, Faisalabad, Pakistan. Electronic address:

Different mutations in the Growth/Differentiation Factor 5 gene (GDF5) have been associated with varying types of skeletal dysplasia, including Grebe type chondrodysplasia (GTC), Hunter-Thompson syndrome, Du Pan Syndrome and Brachydactyly type C (BDC). Heterozygous pathogenic mutations exert milder effects, whereas homozygous mutations are known to manifest more severe phenotypes. In this study, we report a GDF5 frameshift mutation (c. Read More

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Short- and Long-term Probiotic Effects of Enterococcus hirae Isolated from Fermented Vegetable Wastes on the Growth, Immune Responses, and Disease Resistance of Hybrid Catfish (Clarias gariepinus × Clarias macrocephalus).

Fish Shellfish Immunol 2021 Apr 16. Epub 2021 Apr 16.

Laboratory of Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok, Thailand; Center of Excellence in Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok, Thailand. Electronic address:

This study evaluated the short- and long-term effects of dietary supplementation with Enterococcus hirae strain UPM02 on the growth performance, immunity, and disease resistance of hybrid catfish (Clarias gariepinus × Clarias macrocephalus) against Aeromonas hydrophila infection. In the long-term trial, fingerling fish were fed diets containing 0 (control), 2×10, or 2×10 CFU/g E. hirae UPM02 for 120 days. Read More

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Investigation of in vitro odonto/osteogenic capacity of cannabidiol on human dental pulp cell.

J Dent 2021 Apr 16:103673. Epub 2021 Apr 16.

Graduate Periodontics, School of Dentistry, University of Detroit Mercy, Detroit, MI, 48208, USA. Electronic address:

Introduction: Vital pulp treatment (VPT) maintains tooth vitality with certain dental materials by protecting pulp from noxious stimulation and promoting repair through enhancing cell proliferation/differentiation, migration, and inducing odontogenesis. As a non-psychotropic cannabis constituent, cannabidiol (CBD) process the properties of analgesic, anti-inflammation, and osteogenesis. Therefore, we hypothesize that CBD may induce the odonto/osteogenesis of human dental pulp cells (HDPCs), a critical feature using as effective pulp capping agent for VPT. Read More

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Lianhuaqingwen capsule inhibits influenza-induced bacterial adhesion to respiratory epithelial cells through down-regulation of cell adhesion molecules.

J Ethnopharmacol 2021 Apr 16:114128. Epub 2021 Apr 16.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, PR China; Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macau SAR, PR China. Electronic address:

Ethnopharmacological Relevance: Influenza virus infection is widely believed to cause mild symptoms, but can lead to high mortality and severe disease complicated by secondary bacterial pneumonia. Traditional Chinese medicine (TCM) has been proposed as a promising agent to treat respiratory viral infections. An herbal formula Lianhuaqingwen capsule (LHQW) comprising two prescriptions: Maxing Shigan decoction and Yinqiao San, has been used clinically to treat respiratory infection with immune regulatory effects. Read More

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Qigong exercise enhances cognitive functions in the elderly via an interleukin-6-hippocampus pathway: a randomized active-controlled trial.

Brain Behav Immun 2021 Apr 16. Epub 2021 Apr 16.

State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong; Laboratory of Neuropsychology and Human Neuroscience, The University of Hong Kong, Hong Kong; Institute of Clinical Neuropsychology, The University of Hong Kong, Hong Kong. Electronic address:

Background: Evidence has suggested that exercise protects against cognitive decline in aging, but the recent lockdown measures associated with the COVID-19 pandemic have limited the opportunity for outdoor exercise. Herein we tested the effects of an indoor exercise, Qigong, on neurocognitive functioning as well as its potential neuro-immune pathway.

Methods: We conducted a 12-week randomized active-controlled trial with two study arms in cognitively healthy older people. Read More

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Paricalcitol inhibits oxidative stress-induced cell senescence of the bile duct epithelium dependent on modulating Sirt1 pathway in cholestatic mice.

Free Radic Biol Med 2021 Apr 16. Epub 2021 Apr 16.

Department of Cell Biology, School of Medicine, Taizhou University, Taizhou, PR China. Electronic address:

Background: Clinical studies indicate that vitamin D receptor (VDR) expression is reduced in primary biliary cirrhosis patient livers. However, the mechanism by which activated VDR effect cholestatic liver injury remains unclear.

Methods: Mice were injected intraperitoneally with the VDR agonist paricalcitol or a vehicle 3 days prior to bile duct ligation (BDL) and for 5 or 28 days after surgery. Read More

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Downregulation of TGR5 (GPBAR1) in biliary epithelial cells contributes to the pathogenesis of sclerosing cholangitis.

J Hepatol 2021 Apr 16. Epub 2021 Apr 16.

Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital Düsseldorf, Medical Faculty of Heinrich Heine University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address:

Background&aims: Primary sclerosing cholangitis (PSC) is characterized by chronic inflammation and progressive fibrosis of the biliary tree. The bile acid receptor TGR5 is found on biliary epithelial cells (BECs), where it promotes secretion, proliferation and tight junction integrity. Thus, we speculated that changes in TGR5-expression in BECs may contribute to PSC pathogenesis. Read More

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Collapsing Glomerulopathy as a Complication of Type I Interferon-Mediated Glomerulopathy in a Patient With RNASEH2B-Related Aicardi-Goutières Syndrome.

Am J Kidney Dis 2021 Apr 16. Epub 2021 Apr 16.

Department of Biomedical, Experimental and Clinical Sciences "Mario Serio", University of Firenze, Firenze;; Nephrology and Dialysis Unit, Meyer Children's Hospital, Firenze, Italy. Electronic address:

Aicardi-Goutières syndrome (AGS) is a well-characterised monogenic type I interferonopathy presenting with prominent neurological manifestations. Among extra-neurological features, renal involvement has only been described in one IFIH1-mutated patient who developed membranous nephropathy. The pathogenic role of augmented interferon signalling in tissues other than the central nervous system remains to be elucidated. Read More

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Targeting the NS2B-NS3 protease of tick-borne encephalitis virus with pan-flaviviral protease inhibitors.

Antiviral Res 2021 Apr 16:105074. Epub 2021 Apr 16.

Department of Medical Sciences, Clinical Microbiology, Uppsala University, Uppsala, Sweden. Electronic address:

Tick-borne encephalitis (TBE) is a severe neurological disorder caused by tick-borne encephalitis virus (TBEV), a member of the Flavivirus genus. Currently, two vaccines are available in Europe against TBEV. However, TBE cases have been rising in Sweden for the past twenty years, and thousands of cases are reported in Europe, emphasizing the need for antiviral treatments against this virus. Read More

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Epigenetic modulation and understanding of HDAC inhibitors in cancer therapy.

Life Sci 2021 Apr 16:119504. Epub 2021 Apr 16.

Department of Computer Science and Engineering, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India.

The role of genetic and epigenetic factors in tumor initiation and progression is well documented. Histone deacetylases (HDACs), histone methyl transferases (HMTs), and DNA methyl transferases. (DNMTs) are the main proteins that are involved in regulating the chromatin conformation. Read More

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Development and performance characteristics evaluation of a new Bioelectric Recognition Assay (BERA) method for rapid Sars-CoV-2 detection in clinical samples.

J Virol Methods 2021 Apr 16:114166. Epub 2021 Apr 16.

Laboratory of Hygiene and Epidemiology, University of Thessaly, School of Health Sciences, Faculty of Medicine, Papakyriazi 22, Larissa, Greece. Electronic address:

Introduction: As the second wave of COVID-19 pandemic is in progress the development of fast and cost-effective approaches for diagnosis is essential. The aim of the present study was to develop and evaluate the performance characteristics of a new Bioelectric Recognition Assay (BERA) regarding Sars-CoV-2 detection in clinical samples and its potential to be used as a point of care test.

Materials And Methods: All tests were performed using a custom portable hardware device developed by EMBIO DIAGNOSTICS (EMBIO DIAGNOSTICS Ltd, Cyprus). Read More

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Comparison of four PCR and two point of care assays used in the laboratory detection of SARS-CoV-2.

J Virol Methods 2021 Apr 16:114165. Epub 2021 Apr 16.

Military Medical Centre of the Hungarian Defence Forces, H-1134, Budapest, Róbert Károly krt. 44, Hungary; Semmelweis University - Faculty of Medicine, H-1085, Budapest, Üllői út 26., Hungary. Electronic address:

Seeing the global emergence and the lack of a definitive cure for COVID-19, it is essential to find the most sensitive and specific detection method to identify infected patients in a timely manner. Our paper aims to compare the clinical sensitivity of different commercial RT-qPCR (Genesig, 1copy, DNA-Techonolgy and Charité primer-probe sets), isothermal PCR (Ustar Isothermal Amplification-Real Time Fluorescent Assay) and immunochromatographic antigen detection (BOCREDIT COVID-19 Ag) assays developed to use in laboratory diagnosis of COVID-19. A total of 119 nasopharyngeal swab specimens were collected from symptomatic patients. Read More

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Fast and automated detection of common carbapenemase genes using multiplex real-time PCR on the BD MAX™ system.

J Microbiol Methods 2021 Apr 16:106224. Epub 2021 Apr 16.

Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg, Heidelberg, Germany; Institute of Medical Microbiology and Virology, Medical Faculty, Technische Universität Dresden, Dresden, Germany.

Fast detection of carbapenemases in Gram-negative bacilli is necessary for accurate antibiotic treatment, prevention of further spreading and surveillance purposes. We analyzed the current occurrence of gene variants and designed two multiplex PCRs with hydrolysis probes. The assay was developed for the BD MAX™ system that combines DNA extraction and PCR in a fully automated procedure providing results within 3 h and was evaluated for detection of carbapenemases from bacterial isolates and directly from rectal swabs. Read More

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Development and validation of real-time PCR assays for the detection of Ehrlichia species and E. chaffeensis in clinical specimens.

J Microbiol Methods 2021 Apr 16:106225. Epub 2021 Apr 16.

Rickettsial Zoonoses Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States of America. Electronic address:

Ehrlichiosis, caused by Gram-negative bacteria of the genus Ehrlichia, is considered an emerging infectious disease due to the increasing number of reported cases. Symptoms are non-specific and occur within 1 to 2 weeks following the bite of an infected tick. Confirmatory laboratory diagnostic methods vary in sensitivity and specimen requirements, which can lead to delayed diagnosis. Read More

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Mepolizumab for chronic rhinosinusitis with nasal polyps (SYNAPSE): a randomised, double-blind, placebo-controlled, phase 3 trial.

Lancet Respir Med 2021 Apr 16. Epub 2021 Apr 16.

Department of ENT, Guy's Hospital, London, UK; St Thomas' Hospital, King's College London, London, UK.

Background: Chronic rhinosinusitis with nasal polyps affects approximately 2-4% of the general population, and long-term use of systemic corticosteroids is associated with adverse effects. The aim of this study was to assess the efficacy and safety of mepolizumab in adults with recurrent, refractory severe bilateral chronic rhinosinusitis with nasal polyps.

Methods: SYNAPSE was a randomised, double-blind, placebo-controlled, parallel-group, phase 3 trial done at 93 centres, mainly hospitals, in 11 countries. Read More

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Haemoglobin thresholds to define anaemia in a national sample of healthy children and adolescents aged 1-19 years in India: a population-based study.

Lancet Glob Health 2021 Apr 16. Epub 2021 Apr 16.

Population Council, India Habitat Centre, New Delhi, India.

Background: WHO's haemoglobin cutoffs to define anemia were based on five studies of predominantly White adult populations, done over 50 years ago. Therefore, a general re-examination of the existing haemoglobin cutoffs is warranted for global application, in representative healthy populations of children and adults. Such data are scarce in low-income and middle-income countries; however, a 2019, large-scale, nationally representative survey of children and adolescents aged 0-19 years in India (Comprehensive National Nutrition Survey [CNNS]) offered an opportunity for this re-examination. Read More

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