270,675 results match your criteria clinical samples


Using historical data to facilitate clinical prevention trials in Alzheimer disease? An analysis of longitudinal MCI (mild cognitive impairment) data sets.

Alzheimers Res Ther 2021 May 7;13(1):97. Epub 2021 May 7.

University Department of Geriatric Medicine FELIX PLATTER, Basel, Switzerland.

Background: The Placebo Group Simulation Approach (PGSA) aims at partially replacing randomized placebo-controlled trials (RPCTs), making use of data from historical control groups in order to decrease the needed number of study participants exposed to lengthy placebo treatment. PGSA algorithms to create virtual control groups were originally derived from mild cognitive impairment (MCI) data of the Alzheimer's Disease Neuroimaging Initiative (ADNI) database. To produce more generalizable algorithms, we aimed to compile five different MCI databases in a heuristic manner to create a "standard control algorithm" for use in future clinical trials. Read More

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Monitoring of patients with microdialysis following pancreaticoduodenectomy-the MINIMUM study: study protocol for a randomized controlled trial.

Trials 2021 May 7;22(1):329. Epub 2021 May 7.

Department of Anesthesiology, Division of Emergencies and Critical Care, Oslo University Hospital, Rikshospitalet, Postboks 4950 Nydalen, 0424, Oslo, Norway.

Background: Postoperative pancreatic fistula after pancreatoduodenectomy is a much-feared complication associated with substantial mortality and morbidity. The current standard for diagnosing postoperative pancreatic fistula, besides routine clinical examination, include radiological examinations, analysis of pancreatic drain amylase activity, and routine blood samples. Another promising method is by intraperitoneal microdialysis to monitor intraperitoneal metabolites measured at the pancreaticojejunostomy, thereby detecting what occurs locally, before chemical events can be reflected as measurable changes in systemic blood levels. Read More

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EARN: an ensemble machine learning algorithm to predict driver genes in metastatic breast cancer.

BMC Med Genomics 2021 May 7;14(1):122. Epub 2021 May 7.

Laboratory of Complex Biological Systems and Bioinformatics (CBB), Department of Bioinformatics, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.

Background: Today, there are a lot of markers on the prognosis and diagnosis of complex diseases such as primary breast cancer. However, our understanding of the drivers that influence cancer aggression is limited.

Methods: In this work, we study somatic mutation data consists of 450 metastatic breast tumor samples from cBio Cancer Genomics Portal. Read More

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Identification and validation of a hypoxia-related prognostic and immune microenvironment signature in bladder cancer.

Cancer Cell Int 2021 May 7;21(1):251. Epub 2021 May 7.

Department of Urology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.

Background: Bladder cancer is the leading causes of cancer-associated mortality and seriously affects population health. Hypoxia plays a key role in tumor development and immune escape, which contributes to malignant behaviors.

Methods: In this study, we analyzed the RNA-seq and clinical information of bladder cancer patients from The Cancer Genome Atlas (TCGA) database. Read More

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Checkpoint inhibition in combination with an immunoboost of external beam radiotherapy in solid tumors (CHEERS): study protocol for a phase 2, open-label, randomized controlled trial.

BMC Cancer 2021 May 7;21(1):514. Epub 2021 May 7.

Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium.

Background: While the introduction of checkpoint inhibitors (CPIs) as standard of care treatment for various tumor types has led to considerable improvements in clinical outcome, the majority of patients still fail to respond. Preclinical data suggest that stereotactic body radiotherapy (SBRT) could work synergistically with CPIs by acting as an in situ cancer vaccine, thus potentially increasing response rates and prolonging disease control. Though SBRT administered concurrently with CPIs has been shown to be safe, evidence of its efficacy from large randomized trials is still lacking. Read More

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Clinical and prognostic features of patients with detailed RAS/BRAF-mutant colorectal cancer in Japan.

BMC Cancer 2021 May 7;21(1):518. Epub 2021 May 7.

Cancer Treatment Center, Kansai Medical University Hospital, 2-3-1, Shinmachi, Hirakata, Osaka, 573-1191, Japan.

Background: RAS/BRAF mutations are the most remarkable oncogenic driver mutations in colorectal cancer (CRC) and play an important role in treatment selection. No data are available regarding the clinical and prognostic features of patients with detailed RAS/BRAF-mutant metastatic CRC (mCRC) in Japan.

Methods: A total of 152 chemotherapy-naïve patients with mCRC were included in this study between August 2018 and July 2019. Read More

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Time-of-day effects of consumption of fish oil-enriched sausages on serum lipid parameters and fatty acid composition in normolipidemic adults: A randomized, double-blind, placebo-controlled, and parallel-group pilot study.

Nutrition 2021 Mar 24;90:111247. Epub 2021 Mar 24.

Healthy Food Science Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan; Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan; School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Ibaraki, Japan. Electronic address:

Objectives: The body clock controls diurnal rhythms of nutrient digestion, absorption, and metabolism. Fish oil (FO) contains abundant ω-3 polyunsaturated fatty acids (PUFA), including docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), that are thought to lower triglyceride (TG) levels. This randomized, placebo-controlled, double-blind, parallel-group trial aimed to confirm the effects of the time of FO intake on TG in healthy Japanese adults. Read More

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PM2.5 aggravates NQO1-induced mucus hyper-secretion through release of neutrophil extracellular traps in an asthma model.

Ecotoxicol Environ Saf 2021 May 4;218:112272. Epub 2021 May 4.

Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, the Third People's Hospital of Chengdu, Chengdu 610031, China; Department of Pulmonary and Critical Care Medicine, Chengdu third people's hospital branch of National Clinical Research Center for Respiratory Disease, Affiliated Hospital of ChongQing Medical University, Chengdu 610031, China. Electronic address:

Background: Particulate matter of 2.5 µm or less in diameter (PM2.5) is one of the most complex pollutants in the atmospheric environment and harmful to human health. Read More

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Cargo encapsulated hepatitis E virus-like particles for anti-HEV antibody detection.

Biosens Bioelectron 2021 Apr 22;185:113261. Epub 2021 Apr 22.

Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka, 422-8529, Japan; Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka, 422-8529, Japan. Electronic address:

Viral capsid-nanoparticle hybrid structures incorporating quantum dots (QDs) into virus-like particles (VLPs) constitute an emerging bioinspired type of nanoarchitecture paradigm used for various applications. In the present study, we packed inorganic QDs in vitro into the hepatitis E virus-like particle (HEV-LP) and developed a fluorometric biosensor for HEV antibody detection. Firstly, for the preparation of QDs-encapsulated HEV-LPs (QDs@HEV-LP), the HEV-LPs produced by a recombinant baculovirus expression system were disassembled and reassembled in the presence of QDs using the self-assembly approach. Read More

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Prenatal exposure to pyrethroid and organophosphate insecticides and language development at age 20-36 months among children in the Odense Child Cohort.

Int J Hyg Environ Health 2021 May 4;235:113755. Epub 2021 May 4.

Department of Public Health, University of Southern Denmark, Odense, J.B. Winsløws Vej 17A, 5000, Odense, Denmark; Hans Christian Andersen Children's Hospital, Odense University Hospital, Sdr. Boulevard 29, 5000, Odense C, Denmark.

Background: Prenatal exposure to organophosphate and pyrethroid insecticides has been associated with impaired neurodevelopment. Few longitudinal studies have investigated associations with early language development in populations with mainly low dietary exposure.

Objective: To investigate associations between biomarkers of maternal gestational exposure to organophosphate and pyrethroid insecticides and the child's language development at age 20-36 months in the prospective Odense Child Cohort. Read More

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Evaluation of two commercial kits on the automated ELITe InGenius PCR platform for molecular diagnosis of toxoplasmosis.

J Mol Diagn 2021 May 4. Epub 2021 May 4.

Univ. Montpellier, CNRS, IRD, CHU Montpellier, "MiVEGEC", Department of Parasitology-Mycology & Molecular Biology Pole of the National Reference Center for Toxoplasmosis, , Montpellier, France.

Molecular diagnosis of toxoplasmosis is essential for establishing the diagnosis of congenital contaminations and for primary infection or reactivation of immunocompromised patients. An integrated extraction and real-time PCR-based system is of particular interest in this context. Commercial kits for automated extraction and amplification steps are now available. Read More

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The sputum microbiome and clinical outcomes in patients with bronchiectasis: a prospective observational study.

Lancet Respir Med 2021 May 4. Epub 2021 May 4.

Division of Molecular and Clinical Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK. Electronic address:

Background: Infection is a key component of bronchiectasis pathophysiology. Characterisation of the microbiome offers a higher degree of sensitivity and resolution than does traditional culture methods. We aimed to evaluate the role of the microbiome in determining the risk of exacerbation and long-term outcomes, including all-cause mortality, in bronchiectasis. Read More

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Recovering Individual Genomes from Metagenomes Using MaxBin 2.0.

Curr Protoc 2021 May;1(5):e128

Joint BioEnergy Institute, Emeryville, California.

It is critical to identify individual genomes from microbiomic samples in order to carry out analysis of the microbes. Methods based on existing databases, however, may have limited capabilities in elucidating and quantifying the microbes due to the largely unidentified microbial species in natural or human-associated environments. We thus developed a database-free method, MaxBin 2. Read More

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Repetitive mild traumatic brain injury affects inflammation and excitotoxic mRNA expression at acute and chronic time-points.

PLoS One 2021 7;16(5):e0251315. Epub 2021 May 7.

School of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, Queensland, Australia.

The cumulative effect of mild traumatic brain injuries (mTBI) can result in chronic neurological damage, however the molecular mechanisms underpinning this detriment require further investigation. A closed head weight drop model that replicates the biomechanics and head acceleration forces of human mTBI was used to provide an exploration of the acute and chronic outcomes following single and repeated impacts. Adult male C57BL/6J mice were randomly assigned into one of four impact groups (control; one, five and 15 impacts) which were delivered over 23 days. Read More

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Agonistic autoantibodies against ß2-adrenergic receptor influence retinal microcirculation in glaucoma suspects and patients.

PLoS One 2021 7;16(5):e0249202. Epub 2021 May 7.

Department of Ophthalmology, University of Erlangen-Nürnberg, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany.

Purpose: Agonistic β2-adrenergic receptor autoantibodies (β2-agAAb) have been observed in sera of patients with ocular hypertension and open-angle glaucoma (OAG). They target the β2-receptors on trabecular meshwork, ciliary body and pericytes (Junemann et al. 2018; Hohberger et al. Read More

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Metabolomics study on the Periplocin-induced cardiotoxicity and the compatibility of Periplocin and Panax notoginseng saponins in reducing cardiotoxicity in rats by GC-MS.

J Sep Sci 2021 May 7. Epub 2021 May 7.

State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.

Periplocin, as one of the components of cardiac glycosides in Cortex Periplocae, exhibited cardiotonic effects. Orally ingesting periplocin in high doses or over prolonged periods would cause serious adverse reactions, especially cardiotoxicity, which limits the applications of periplocin in clinical therapy. It has been reported that panax notoginseng saponins could be used in compatibility with periplocin, to reduce the cardiotoxicity of periplocin. Read More

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Role of calcium phosphate and bioactive glass coating on in vivo bone healing of new Mg-Zn-Ca implant.

J Mater Sci Mater Med 2021 May 7;32(5):55. Epub 2021 May 7.

Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, India.

Present investigation focuses on development and detailed characterization of a new Mg alloy sample (BM) with and without coating of hydroxyapatite (BMH) and bioactive glass (BMG) by air plasma spray method. After detailed mechano-physico-chemical characterization of powders and coated samples, electrochemical corrosion and SBF immersion tests were carried out. Detailed in vitro characterizations for cell viability were undertaken using MG-63 cell line followed by in vivo tests in rabbit model for studying bone healing up to 60 days. Read More

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Effects of LI05 on immunity and metabolism in germ-free rats.

Food Funct 2021 May 7. Epub 2021 May 7.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Many Pediococcus spp. have health-promoting benefits, and Pediococcus pentosaceus LI05 is one such species that was proved to be beneficial in previous studies. Our research aimed to determine the immune and metabolic effects of P. Read More

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Investigation of Cytomegalovirus in Intestinal Tissue in a Country With High CMV Seroprevalence.

Turk J Gastroenterol 2021 Feb;32(2):123-132

Department of Medical Microbiology, Dokuz Eylül University School of Medicine, İzmir, Turkey.

Background: In Turkey, cytomegalovirus (CMV) seropositivity has been reported to be high, between 85 and 100%. CMV has been responsible for disease exacerbation in inflammatory bowel disease (IBD). We aimed to evaluate the presence of CMV in intestinal tissue by immunohistochemical staining in IBD and non-IBD patient groups, in a country with high CMV seroprevalence. Read More

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February 2021

Acute phenthoate self-poisoning: a prospective case series.

Clin Toxicol (Phila) 2021 May 7:1-7. Epub 2021 May 7.

Pharmacology, Toxicology, & Therapeutics, University/BHF Centre for Cardiovascular Science, Edinburgh, UK.

Background: The clinical characteristics following self-poisoning with organophosphorus (OP) insecticides differs according to the insecticide ingested. Phenthoate is a dimethoxy WHO Hazard Class II OP pesticide with limited literature on its clinical characteristics and outcome. We aimed to better understand its clinical characteristics by studying patients with phenthoate self-poisoning in Sri Lanka. Read More

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Real-world utility of next-generation sequencing for targeted gene analysis and its application to treatment in lung adenocarcinoma.

Cancer Med 2021 May 7. Epub 2021 May 7.

Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

Purpose: This study investigated the clinical utility of next-generation sequencing (NGS) for detection of genetic alterations and its implications on treatment of lung adenocarcinoma in real-world practice.

Patients And Methods: Data were reviewed for 391 patients with lung adenocarcinoma who underwent NGS between March 2017 and October 2018. Formalin-fixed, paraffin-embedded archival samples were used for performing NGS targeting 382 genes, including all exons of 199 genes, 184 hotspots, and the partial introns of 8 genes often rearranged in cancer. Read More

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The debatable presence of PIWI-interacting RNAs in invasive breast cancer.

Cancer Med 2021 May 7. Epub 2021 May 7.

School of Medicine, Institute of Clinical Medicine, Pathology and Forensic Medicine, and Translational Cancer Research Area, University of Eastern Finland, Kuopio, Finland.

Numerous factors influence breast cancer (BC) prognosis, thus complicating the prediction of outcome. By identifying biomarkers that would distinguish the cases with poorer response to therapy already at the time of diagnosis, the rate of survival could be improved. Lately, Piwi-interacting RNAs (piRNAs) have been introduced as potential cancer biomarkers, however, due to the recently raised challenges in piRNA annotations, further evaluation of piRNAs' involvement in cancer is required. Read More

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Capillary blood sampling increases the risk of preanalytical errors in pediatric hospital care: Observational clinical study.

J Spec Pediatr Nurs 2021 May 7:e12337. Epub 2021 May 7.

Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden.

Purpose: The blood sampling procedure is complex and prone to failure, as reflected by preanalytical errors in pediatric hospital care. The primary aim was to evaluate if the risk of preanalytical errors was higher with capillary blood sampling than with venous blood sampling, and secondary, explore specific factors associated with preanalytical errors, both overall and stratified by capillary and venous blood sampling.

Design And Methods: This observational pediatric hospital study collected outcomes from medical records and blood sampling surveys from year 2014 to 2016. Read More

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Validation of the Munich Actimetry Sleep Detection Algorithm for estimating sleep-wake patterns from activity recordings.

J Sleep Res 2021 May 7:e13371. Epub 2021 May 7.

Institute of Medical Psychology, Ludwig Maximilian University Munich, Munich, Germany.

Periods of sleep and wakefulness can be estimated from wrist-locomotor activity recordings via algorithms that identify periods of relative activity and inactivity. Here, we evaluated the performance of our Munich Actimetry Sleep Detection Algorithm. The Munich Actimetry Sleep Detection Algorithm uses a moving 24-h threshold and correlation procedure estimating relatively consolidated periods of sleep and wake. Read More

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Whole exome sequencing identifies functional classes of gene mutations associated with bone marrow failure in pediatric Fanconi Anemia patients.

Eur J Haematol 2021 May 7. Epub 2021 May 7.

The Norris Cotton Cancer Center, Department of Pediatrics, Geisel School of Medicine at Dartmouth College, Lebanon, NH, USA.

Fanconi anemia (FA) is an inherited bone marrow failure syndrome characterized by biallelic germline mutations in FA genes in the DNA damage repair pathway. Defective DNA damage repair leading to genomic instability suggests a role of acquired somatic mutations in disease progression to bone marrow failure (BMF). We hypothesize that the somatic mutational landscape of FA patients can predict the development of BMF, the most common cause of morbidity and mortality in this patient population. Read More

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Accelerating Medicines Partnership: Parkinson's Disease. Genetic Resource.

Mov Disord 2021 May 7. Epub 2021 May 7.

Center for Alzheimer's and Related Dementias, National Institute on Aging, Bethesda, Maryland, USA.

Background: Whole-genome sequencing data are available from several large studies across a variety of diseases and traits. However, massive storage and computation resources are required to use these data, and to achieve sufficient power for discoveries, harmonization of multiple cohorts is critical.

Objectives: The Accelerating Medicines Partnership Parkinson's Disease program has developed a research platform for Parkinson's disease (PD) that integrates the storage and analysis of whole-genome sequencing data, RNA expression data, and clinical data, harmonized across multiple cohort studies. Read More

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A graphene oxide coated tapered microfiber acting as a super-sensor for rapid detection of SARS-CoV-2.

Lab Chip 2021 May 7. Epub 2021 May 7.

Shanghai Key Laboratory of Modern Optical System, Engineering Research Center of Optical Instrument and System (Ministry of Education), School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

COVID-19 is a new strain of highly contagious coronavirus, and at present, more than 221.4 million people have been infected with this virus, and the death toll exceeds 2793398. Early and fast detection of COVID-19 from infected individuals is critical to limit its spreading. Read More

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The commensal consortium of the gut microbiome is associated with favorable responses to anti-programmed death protein 1 (PD-1) therapy in thoracic neoplasms.

Cancer Biol Med 2021 May 7. Epub 2021 May 7.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Objective: Immune checkpoint inhibitors have revolutionized cancer therapy for multiple types of solid tumors, but as expected, a large percentage of patients do not show durable responses. Biomarkers that can predict clinical responses to immunotherapies at diagnosis are therefore urgently needed. Herein, we determined the associations between baseline gut commensal microbes and the clinical treatment efficiencies of patients with thoracic neoplasms during anti-programmed death protein 1 (PD-1) therapy. Read More

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Discordant SARS-CoV-2 Detection in the Nasopharynx versus Trachea for Patients with Tracheostomies.

Laryngoscope 2021 May 6. Epub 2021 May 6.

Department of Otolaryngology - Head and Neck Surgery, University of Michigan, 1500 E Medical Center Dr, Ann Arbor, Michigan, 48109.

Objective: Patients with tracheostomies have an anatomically altered connection between their upper and lower airways that could impact SARS-CoV-2 testing. Our goal was to evaluate for discordance in SARS-CoV-2 detection in hospitalized patients with COVID-19 and tracheostomies based on the site analyzed.

Methods: This single-institution study evaluated hospitalized patients with COVID-19 who had tracheostomies placed during their treatment. Read More

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Diagnosis of Fluorosis by Analysis of Fluoride Content in Body Fluids Using Ion Selective Electrode Method.

Authors:
N K Mondal

Adv Exp Med Biol 2021 ;1306:121-127

Fluorosis Foundation of India (Camping & Working for JICA Funded 'Fluorosis Mitigation Programme' in Nagaur District, Rajasthan, India), New Delhi, India.

Analysis of fluoride in body fluids (urine and serum) is essential for fluorosis diagnosis. Although 24-h urine collection is adopted to assess community defluoridation/fluoride supplementation program/research studies, but not feasible for Clinical/Pathological laboratories. Patients are reluctant to bring 24-h urine samples. Read More

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January 2021