1,185 results match your criteria cl-6b chromatography

Kirkiin: A New Toxic Type 2 Ribosome-Inactivating Protein from the Caudex of .

Toxins (Basel) 2021 Jan 22;13(2). Epub 2021 Jan 22.

Department of Experimental, Diagnostic and Specialty Medicine-DIMES, General Pathology Section, Alma Mater Studiorum-University of Bologna, 40126 Bologna, Italy.

Ribosome-inactivating proteins (RIPs) are plant toxins that irreversibly damage ribosomes and other substrates, thus causing cell death. RIPs are classified in type 1 RIPs, single-chain enzymatic proteins, and type 2 RIPs, consisting of active A chains, similar to type 1 RIPs, linked to lectin B chains, which enable the rapid internalization of the toxin into the cell. For this reason, many type 2 RIPs are very cytotoxic, ricin, volkensin and stenodactylin being the most toxic ones. Read More

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January 2021

Immunomodulatory effect of polysaccharides from the extraction of (Blume) Hook. f. et Thomson (Campanulaceae) roots in female rats.

Nat Prod Res 2020 Aug 3:1-5. Epub 2020 Aug 3.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medcial University, Guiyang, PR China.

In this study, the crude polysaccharides of (CJP) was obtained from (Blume) Hook. f. et Thomson using hot water extraction method, which was separated and purified by DEAE-cellulose column and Sepharose CL-6B column. Read More

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Novel thermostable lipase produced by a thermo-halophilic bacterium that catalyses hydrolytic and transesterification reactions.

Heliyon 2020 Jul 24;6(7):e04520. Epub 2020 Jul 24.

Biomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, Indonesia.

Lipase belongs to the class of hydrolytic enzymes that are widely used in the biotechnology industries. The goal of this research was to purify and characterize lipase produced from a thermo-halophilic bacterium, namely Pria Laot Sabang 80 (PLS 80). Purification was performed using ammonium sulphate fractionation, followed by gel filtration chromatography using Sepharose Cl-6B. Read More

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Effect of tween 40 and ethanol on the secretion, structure and antioxidant activities of exopolysaccharides from Inonotus rickii.

An Acad Bras Cienc 2020 27;92 Suppl 2:e20180838. Epub 2020 Jul 27.

School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, China.

In this study, the effects of Tween 40 and ethanol supplementation on the secretion, structure and antioxidant activities of exopolysaccharide (EPS) from Inonotus rickii were investigated. It was observed that Tween 40 and ethanol displayed a stimulatory effect on EPS secretion. The EPSs obtained by the addition of Tween 40 (EPS-T), ethanol (EPS-E) and control (EPS-C) were purified by Sepharose CL-6B gel chromatography and molecular weights of EPS-T, EPS-E and EPS-C were estimated to be 22. Read More

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September 2020

Structural characterization and immunomodulatory activity of a polysaccharide from Eurotium cristatum.

Int J Biol Macromol 2020 Nov 13;162:609-617. Epub 2020 Jun 13.

Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China; Biotech & Biomed Research Institute, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China. Electronic address:

By combining DEAE-cellulose-52 and Sepharose-CL-6B chromatography, a heteropolysaccharide ECIP-1A with an average molecular weight of 24,336 Da was isolated from the hyphae of Eurotium cristatum. The main components of the monosaccharides of ECIP-1A were mannose, glucose and galactose, and their molar ratio is 4.003: 0. Read More

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November 2020

Characterization and comparison of two peptide-tag specific nanobodies for immunoaffinity chromatography.

J Chromatogr A 2020 Aug 25;1624:461227. Epub 2020 May 25.

Liaoning Key Laboratory of Molecular Recognition and Imaging, School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Dalian 116023, PR China. Electronic address:

Affinity chromatography is generally regarded as a powerful tool allowing the single step purification of recombinant proteins with high purity and yields. However, for most protein products, affinity purification methods for industrial applications are not readily available, mainly due to the lack of specific and robust natural counterparts that could function as affinity ligands. In this study, we explored the applicability of nanobody-based peptide-tag immunorecognition systems as a platform for affinity chromatography. Read More

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Purification and Assays of Tachylectin-2.

Methods Mol Biol 2020 ;2132:309-316

Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.

Tachylectin-2, a 27-kDa protein consisting of a five-bladed β-propeller structure, is purified by three steps of chromatography, including dextran sulfate-Sepharose CL-6B, CM-Sepharose CL-6B, and Mono S. Three isolectins of tachylectin-2 including tachylectin-2a, -2b, and -2c are purified. These isolectins exhibit hemagglutinating activity against human A-type erythrocytes in a Ca-independent manner with tachylectin-2b showing the highest activity. Read More

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Characterization of carbaryl-degrading strain Bacillus licheniformis B-1 and its hydrolase identification.

Biodegradation 2020 04 18;31(1-2):139-152. Epub 2020 Apr 18.

College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, 625014, People's Republic of China.

Pesticides introduced inadvertently or deliberately into environment by anthropogenic activity have caused growing global public concern, therefore the search of approaches for elimination of such xenobiotics should be encouraged. A cypermethrin-degrading bacterial strain Bacillus licheniformis B-1 was found to efficiently degrade carbaryl in LB medium at concentrations of 50-300 mg L within 48 h, during which temperature and pH played important roles as reflected by increase in pollutant depletion. A stimulatory effect of Fe and Mn on microbial growth was observed, whereas Cu caused inhibition of degradation. Read More

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Study of imidazole performance as pseudo-affinity ligand in the purification of IgG from bovine milk.

Anal Biochem 2020 05 19;597:113693. Epub 2020 Mar 19.

Department of Organic Chemistry and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.

The spherical sepharose CL-6B beads were activated by epichlorohydrin in different epoxy contents (80, 120 and 160 μmol/mL) and, l-histidine and imidazole as pseudo-affinity ligands were covalently immobilized to them. Some linkers with different length, (1,2-ethanediol diglycidyl ether and 1,4-butanediol diglycidyl ether) were synthesized for activation of sepharose and the activated sepharose beads modified with imidazole and the performance of these adsorbents in the purification of immunoglobulin G from bovine milk were evaluated. Among the l-histidine bearing adsorbents, higher adsorption of IgG (0. Read More

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Evaluating the ion exchange chromatography for matrix-assisted PEGylation and purification of consensus interferon.

Biotechnol Appl Biochem 2020 Mar 3;67(2):196-205. Epub 2019 Nov 3.

National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.

Scientists have implemented protein-PEGylation technology for boosting-up the pharmacokinetics and stability of recombinant therapeutic proteins. In the present study, (a) matrix-assisted PEGylation was compared with solution-phase PEGylation and (b) matrix-assisted PEGylation was performed with different ion exchange resins for impact of chromatography medium on yield and purity of PEGylated product. DEAE Sepharose CL 6B, DEAE Fracto gel, and Macro cap Q ion exchange chromatography medium were compared for on column PEGylation and purification of cIFN. Read More

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Synthesis and characterization of crustin capped titanium dioxide nanoparticles: Photocatalytic, antibacterial, antifungal and insecticidal activities.

J Photochem Photobiol B 2019 Oct 3;199:111620. Epub 2019 Sep 3.

Biomaterials and Biotechnology in Animal Health Lab, Nanobiosciences and Nanopharmacology Division, Department of Animal Health and Management, Alagappa University, Karaikudi 630004, Tamil Nadu, India. Electronic address:

Current scenario of bio-nanotechnology, successfully fabrication of ultrafine titanium dioxide nanoparticles (TiONPs) using various biological protein sources for the multipurpose targets. The present research report involves synthesis of TiONPs using antimicrobial peptide (AMP) crustin (Cr). Crustin previously purified from the blue crab, Portunus pelagicus haemolymph, by blue Sepharose CL-6B matrix assisted affinity column chromatography. Read More

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October 2019

Optimization of ultrasonic-assisted enzymatic extraction of polysaccharides from thick-shell mussel (Mytilus coruscus) and their antioxidant activities.

Int J Biol Macromol 2019 Nov 16;140:1116-1125. Epub 2019 Aug 16.

Zhejiang Marine Development Research Institute, Zhoushan, China.

This study aimed to obtain the purified fractions of Mytilus coruscus polysaccharides (MCPs) and investigate their antioxidant activities. MCPs were prepared through ultrasonic-assisted enzymatic extraction optimized by employing the response surface methodology. A single-factor experiment was conducted using the Box-Behnken design to determine the optimum extraction conditions of MCPs. Read More

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November 2019

Bci528I, a new isoschizomer of EcoRI isolated from Bacillus circulans 528.

Folia Microbiol (Praha) 2019 Nov 1;64(6):803-808. Epub 2019 Apr 1.

Korean Center for Culture Collection, Pyongyang, Democratic People's Republic of Korea.

Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Read More

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November 2019

Optimization, purification, and characterization of hydroxylamine oxidoreductase from Acinetobacter sp. Y1.

Biotechnol Appl Biochem 2019 Jul 1;66(4):494-501. Epub 2019 Apr 1.

Key Laboratory of Coal Science and Technology of Shanxi Province and Ministry of Education, Taiyuan University of Technology, Taiyuan, Shanxi, People's Republic of China.

Hydroxylamine oxidoreductase (HAO) is a key enzyme involved in ammonium removal pathway. To further study the enzyme, HAO was purified from heterotrophic nitrifier Acinetobacter sp. Y1 and its property was investigated. Read More

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Isolation of a putative virulence agent, cytotoxic serine-elastase, from a newly isolated ZuhP13.

J Biosci 2019 Mar;44(1)

Department of Microbiology and Botany, Faculty of Science, Zagazig University, Zagazig 44519, Egypt,

A 48 kDa ZuhP13 elastase from P. aeruginosa isolated from a urine sample was successfully purified to 8.8-fold and 39% recovery by DEAE-Sepharose CL-6B and Sephadex G-100 chromatography. Read More

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Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115.

BMC Pharmacol Toxicol 2018 Aug 23;19(1):51. Epub 2018 Aug 23.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.

Background: L-asparaginase is a potential therapeutic enzyme widely used in the chemotherapy protocols of pediatric and adult patients with acute lymphoblastic leukemia. However, its use has been limited by a high rate of hypersensitivity in the long-term used. Hence, there is a continuing need to search for other L-asparaginase sources capable of producing an enzyme with less adverse effects. Read More

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Searching for crab-borne antimicrobial peptides: Crustin from Portunus pelagicus triggers biofilm inhibition and immune responses of Artemia salina against GFP tagged Vibrio parahaemolyticus Dahv2.

Mol Immunol 2018 09 30;101:396-408. Epub 2018 Jul 30.

Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India; Department of Zoology, Government College for Women, Kumbakonam, 612 001, Tamil Nadu, India.

Marine organisms represent a huge source of novel compounds for the development of effective antimicrobial drugs. The present study focus on the purification of the antimicrobial peptide crustin from the haemolymph of the blue swimmer crab, Portunus pelagicus, by blue Sepharose CL-6B matrix assisted affinity column chromatography. Crustin showed a single band with a molecular mass of 17 kDa in SDS-PAGE analysis. Read More

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September 2018

Effects of bed compression on protein separation on gel filtration chromatography at bench and pilot scale.

J Chem Technol Biotechnol 2018 Jul 9;93(7):1959-1965. Epub 2017 Oct 9.

Ipsen Limited Wrexham UK.

Background: Poorly packed chromatography columns are known to reduce drastically the column efficiency and produce broader peaks. Controlled bed compression has been suggested to be a useful approach for solving this problem. Here the relationship between column efficiency and resolution of protein separation are examined when preparative chromatography media were compressed using mechanical and hydrodynamic methods. Read More

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Characterization and Antioxidant Activity of an Exopolysaccharide Produced by Rigidoporus microporus (Agaricomycetes).

Int J Med Mushrooms 2018 ;20(4):311-320

College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, China; Collaborative Innovation Center of Food Production and Safety, Zhengzhou, Henan, China.

In this investigation we developed an optimal fermentation medium to produce an exopolysaccharide (EPS) with antioxidant activity from Rigidoporus microporus. Lactose and tryptone were chosen as the optimal carbon and nitrogen sources, respectively, for EPS production, with a 6-day cultivation cycle. After removing proteins through the use of the Sevag method, one EPS fraction was purified from the culture filtrates by gel filtration chromatography on a Sepharose CL-6B column. Read More

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November 2018

Rhizoctonia solani fucomannogalactan: Chemical characterization and antiproliferative activity.

Int J Biol Macromol 2018 Aug 10;115:106-113. Epub 2018 Apr 10.

São Paulo State University (UNESP), Department of Microbiology and Immunology, Institute of Biosciences, Botucatu 18618-689, Brazil.

A fucomannogalactan from Rhizoctonia solani biomass was obtained after hot aqueous extraction and purified by freeze-thaw cycles and gel filtration chromatography on Sepharose CL-6B. The polysaccharide was homogeneous after HPSEC/RID analysis (M/M~1.1), displaying an average molecular weight of 15. Read More

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Optimization of ultrasound-assisted extraction of okra (Abelmoschus esculentus (L.) Moench) polysaccharides based on response surface methodology and antioxidant activity.

Int J Biol Macromol 2018 Jul 4;114:1056-1063. Epub 2018 Apr 4.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; National Engineering Research Center for Fruits and Vegetables Processing, Beijing 100083, China; Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Beijing 100083, China. Electronic address:

This study determined the optimal conditions for ultrasound-assisted extraction of a water-soluble polysaccharide, Raw Okra Polysaccharide, from the fruit of okra using response surface methodology. The optimal extraction temperature, extraction time and ultrasonic power were 59°C, 30min and 522W, respectively, giving a yield of 10.35±0. Read More

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Identification, characterization and immune response of prophenoloxidase from the blue swimmer crab Portunus pelagicus and its antibiofilm activity.

Int J Biol Macromol 2018 Jul 7;113:996-1007. Epub 2018 Mar 7.

Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Kozep Fasor 52, H-6726 Szeged, Hungary.

Prophenoloxidase is a conserved Cu-containing enzyme acting as a major defense molecule in the immune response of crustaceans. In the present research, we purified prophenoloxidase from the haemolymph of Portunus pelagicus (Pp-proPO) by Blue Sepharose CL-6B chromatography. Pp-proPO exhibited only one band with molecular weight of 75kDa on SDS-PAGE. Read More

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Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.

Molecules 2018 Mar 6;23(3). Epub 2018 Mar 6.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China.

Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 ± 0. Read More

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[PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications].

Prikl Biokhim Mikrobiol 2017 Mar-Apr;53(2):164-72

N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. Read More

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Total fractionation and characterization of the water-soluble polysaccharides isolated from Enteromorpha intestinalis.

Int J Biol Macromol 2018 May 8;111:319-325. Epub 2018 Jan 8.

Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, Guangxi 541004, China. Electronic address:

Water-soluble crude polysaccharides (WE) were extracted from the Enteromorpha intestinalis with hot water and further fractionated on a DEAE-52 Cellulose chromatography column and Sepharose CL-6B gel-permeation chromatography to afford one neutral fraction (WE-11) and five acidic fractions (WE-21, WE-31, WE-32, WE-41 and WE-42). Monosaccharide analysis showed that WE-32, WE-41 and WE-42 were all composed of Rha and GlcA, WE-21 and WE-31 contained Man and Rha, and WE-11 was composed of GlcA and Xyl. The molecular weight of WE-11, WE-21, WE-31 WE-32, WE-41 and WE-42 were 2. Read More

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Structural Characterization and In Vitro Antitumor Activity of a Novel Exopolysaccharide from Lachnum YM130.

Appl Biochem Biotechnol 2018 Jun 8;185(2):541-554. Epub 2017 Dec 8.

Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.

Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 10 Da, which was consisted of mannose and galactose in a molar ratio of 3. Read More

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Preparation and Bioactivity of Exopolysaccharide from an Endophytic Fungus sp. of the Medicinal Plant Gynostemma Pentaphylla.

Pharmacogn Mag 2017 Jul-Sep;13(51):477-482. Epub 2017 Jul 19.

College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P. R. China.

Background: Many exopolysaccharides from the endophytes in medicinal plants possess various potential bioactivities.

Materials And Methods: The endophytic fungus JY25 was isolated from the leave of the Chinese medicinal plant and identified as sp. by its phylogenetic and physiological analysis. Read More

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Purification, characterization, and bioactivities of a polysaccharide from mycelial fermentation of Bjerkandera fumosa.

Carbohydr Polym 2017 Jul 10;167:115-122. Epub 2017 Mar 10.

School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address:

In this work, a novel polysaccharide (named DBFM3) was isolated from mycelia of Bjerkandera fumosa by DEAE-32 and Sepharose CL-6B column chromatography. High-performance gel permeation chromatography showed that DBFM3 was homogeneous, with an average molecular weight of 1.8×10Da. Read More

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Purification, characterization and amino acid content of cholesterol oxidase produced by Streptomyces aegyptia NEAE 102.

BMC Microbiol 2017 03 29;17(1):76. Epub 2017 Mar 29.

Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt.

Background: There is an increasing demand on cholesterol oxidase for its various industrial and clinical applications. The current research was focused on extracellular cholesterol oxidase production under submerged fermentation by a local isolate previously identified as Streptomyces aegyptia NEAE 102. The crude enzyme extract was purified by two purification steps, protein precipitation using ammonium sulfate followed by ion exchange chromatography using DEAE Sepharose CL-6B. Read More

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A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix.

Sci Rep 2017 02 21;7:42849. Epub 2017 Feb 21.

Department of Biological Sciences, University of Calgary, 2500 University Dr., N.W. Calgary, Alberta, T2N 1N4, Canada.

SAVSBPM18 is an engineered streptavidin for affinity purification of both biotinylated biomolecules and recombinant proteins tagged with streptavidin binding peptide (SBP) tags. To develop a user-friendly approach for the preparation of the SAVSBPM18-based affinity matrices, a designer fusion protein containing SAVSBPM18 and a galactose binding domain was engineered. The galactose binding domain derived from the earthworm lectin EW29 was genetically modified to eliminate a proteolytic cleavage site located at the beginning of the domain. Read More

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February 2017