8,234 results match your criteria chromatography resin

Multistage fractionation of pine bark by liquid and supercritical carbon dioxide.

Bioresour Technol 2021 Aug 30;341:125862. Epub 2021 Aug 30.

University of Natural Resources and Life Sciences (BOKU, Vienna), Department of Chemistry, Institute of Chemistry of Renewable Resources, Konrad-Lorenz-Straße 24, A-3430 Tulln, Austria. Electronic address:

Multistage fractionation of pine bark was performed using subcritical and supercritical CO at increasing pressures and temperatures. In total, seven fractions were collected, which demonstrated different enrichments of families of compounds. In particular, subcritical CO yielded 41% of the total extract in which unsaturated fatty acids represented the most abundant family. Read More

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Characterization of a macroporous epoxy-polymer based resin for the ion-exchange chromatography of therapeutic proteins.

J Chromatogr A 2021 Sep 1;1656:462503. Epub 2021 Sep 1.

Department of Material Chemistry, Kyoto Institute of Technology, 1 Hashigami-Cho, Matsugasaki, Sakyo-ku, Kyoto, Other, 606-8585, Japan. Electronic address:

This study investigated the adsorption capacity and mass transfer properties of a novel macroporous epoxy-polymer-based anion-exchanger, MPR Q, for the efficient separation of therapeutic proteins. MPR Q resin was prepared by phase separation based on spinodal decomposition followed by dextran grafting and ligand conjugation. Under static conditions, MPR Q exhibited a binding capacity of 49. Read More

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September 2021

Miniaturized multiresidue method for determination of 267 pesticides, their metabolites and polychlorinated biphenyls in low mass beebread samples by liquid and gas chromatography coupled with tandem mass spectrometry.

Talanta 2021 Dec 20;235:122721. Epub 2021 Jul 20.

Department of Pharmacology and Toxicology, National Veterinary Research Institute, Aleja Partyzantów 57, 24-100, Puławy, Poland.

Current work presents developed and validated miniaturized method for residue analysis of 261 pesticides and their metabolites as well as 6 congeners of non-dioxin like polychlorinated biphenyls (ndl-PCB) in a very low mass beebread sample. Sample preparation is based on modified QuEChERS protocol with all steps miniaturized to enable multiresidue analysis of sample with extremely low weight. Sample of beebread (0. Read More

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December 2021

Traceability of VOCs in tire inner liner by chromatography-mass spectrometry.

Environ Sci Pollut Res Int 2021 Sep 9. Epub 2021 Sep 9.

The Key Laboratory of Beijing on Preparation and Processing of Novel Polymer Materials, Beijing University of Chemical Technology, Beijing, 100029, China.

Many parts of the vehicle cabin generate volatile organic compounds (VOCs), and some are hazardous and/or odorous to humans. In this study, VOCs in the inner liner of automobile spare tire, including raw rubbers and resins, were detected by gas chromatography-mass spectrometry (GC-MS) coupled with an extracting method of static headspace sampling (SHS). The results demonstrated that the sources of VOCs can be traced back to raw rubbers and resins: alkylphenol resins can release a large amount of 2,4,4-trimethyl-1-pentene and 2,2,4,6,6-pentamethyl-3-heptene; chlorobutyl rubber (CIIR) contained 3-methyl-pentane, and methyl-cyclopentane, and these VOCs are odorous. Read More

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September 2021

Quantification of phytic acid in baby foods by derivatization with (trimethylsilyl)diazomethane and liquid chromatography-mass spectrometry analysis.

Rapid Commun Mass Spectrom 2021 Sep 8:e9194. Epub 2021 Sep 8.

College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, Hangzhou.

Rationale: Phytic acid (PA) is both a naturally occurring nutrient and a widely-used food additive for conferring antioxidant properties to food. PA can be found in baby foods and it is essential to monitor PA content due to its antinutritional properties when present in excess. Current methods for determining PA content are unsatisfactory because interference from inositol phosphates and inorganic phosphates complicate PA quantification. Read More

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September 2021

Characterization and determination of the quality of rosins and turpentines extracted from and resin.

Heliyon 2021 Aug 20;7(8):e07834. Epub 2021 Aug 20.

University of Cauca, Faculty of Exact Natural Sciences and Education, Department of Chemistry, QPN Group, PhD, Popayán, Colombia.

Rosins and turpentines present in pine resins have been widely used both industrially (coatings, electronics and paint) and pharmaceuticals (ointments). Among the main components of these matrices is abietic acid. This study focuses on the characterization and evaluation of the quality of rosins and turpentines in addition to the quantification of abietic acid in rosins. Read More

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Long-term elution of bisphenol A from dental composites.

Dent Mater 2021 Sep 2. Epub 2021 Sep 2.

KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven (UZ Leuven), Dentistry, Leuven, Belgium. Electronic address:

Objectives: BPA release from composites on the short term has been reported in several in-vitro and in-vivo studies. However, it remains unclear whether these materials also leach BPA on the long term. Even though composites may release various (BPA-based) methacrylate monomers up to one year, quantitative data about BPA have not been reported due to the lack of a sensitive method to accurately quantify low levels of BPA. Read More

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September 2021

Structure-Indicated LC-MS/MS Bioanalysis of Therapeutic Antibodies.

Methods Mol Biol 2022 ;2313:187-205

Shimadzu Bioscience Research Partnership, Shimadzu Scientific Instruments, Bothell, WA, USA.

Monoclonal antibodies bind to Protein A/G resin with 100 nm-diameter pores, which orients the Fab toward the reaction solution. Then, they can be proteolyzed using trypsin immobilized on the surface of 200 nm-diameter nanoparticles. The difference between the two particle diameters allows Fab-selective proteolysis by limiting trypsin access to the antibody substrate. Read More

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January 2022

Purification of Therapeutic Antibodies by Protein A Affinity Chromatography.

Methods Mol Biol 2022 ;2313:169-177

Department of Chemical Engineering, Indian Institute of Technology Delhi, New Delhi, India.

Protein A affinity chromatography is widely used for capturing therapeutic antibodies. It offers high binding capacity, selectivity, and resin reusability while delivering high yield and product purity. The Protein A step is ubiquitous in its presence in purification platforms for production of antibody products due to the effective clearance it offers of impurities, high and low molecular weight species (HMW and LMW), host cell proteins (HCP), and DNA. Read More

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January 2022

[Bibenzyls from Dendrobium officinale].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(15):3853-3858

The Ministry of Education Key Laboratory for Standardization of Chinese Medicines, The State Administration of Traditional Chinese Medicine Key Laboratory for New Resources and Quality Evaluation of Chinese Medicine, Shanghai R&D Center for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.

Fifteen bibenzyls were isolated and purified from the ethyl acetate extract of the stems of Dendrobium officinale by macroporous resin, MCI, silica gel, Sephadex LH-20, and ODS column chromatographies, as well as preparative thin-layer chromatography and preparative HPLC. The structures of compounds were identified according to the spectra data of ~1H-NMR, ~(13)C-NMR, and MS, and the physical and physiochemical properties: dendrocandin X(1), 3,4'-dihydroxy-4,5-dimethoxybibenzyl(2), 6″-de-O-methyldendrofindlaphenol A(3), 3,4-dihydroxy-4',5-dimethoxybibenzyl(4), dendrosinen B(5), 3,4,4'-trihydroxy-5-methoxybibenzyl(6), 3,3'-dihydroxy-4,5-dimethoxybibenzyl(7), 3,4'-dihydroxy-5-methoxybibenzyl(8), moscatilin(9), gigantol(10), 4,4'-dihydroxy-3,5-dimethoxybibenzyl(11), 3,4',5-trihydroxy-3'-methoxybibenzyl(12), 3-O-methylgigantol(13), dendrocandin U(14), and dendrocandin N(15). Compound 1 was a novel compound. Read More

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[A new labdane-type diterpenoids from Callicarpa nudiflora].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(16):4139-4144

Jiangzhong Pharmaceutical Co., Ltd. Nanchang 330004, China.

The purpose of the research is to study the bioactive constituents of Callicarpa nudiflora. From the 65% ethanol extract of C. nudiflora leaves, ten compounds were isolated by macroporous adsorption resin, Sephadex LH-20, ODS, silica gel, and preparative HPLC. Read More

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[Purification and component identification of total proanthocyanidins in Choerospondias axillaris pericarp].

Zhongguo Zhong Yao Za Zhi 2021 Jun;46(12):2923-2930

Jiangxi Qiyunshan Food Co., Ltd. Ganzhou 341000, China.

The present study determined the quantitative markers of total proanthocyanidins in the purification of the industrial waste Choerospondias axillaris pericarp based on the comparison results of high-performance liquid chromatography(HPLC) and mass spectrometry(MS) and optimized the purification process with two stable procyanidins as markers. The adsorption and desorption of five different macroporous adsorption resins, the static adsorption kinetics curve of NKA-Ⅱ resin, the maximum sample load, and the gradient elution were investigated. The UPLC-Q-TOF-MS/MS was employed for qualitative analysis of the newly-prepared total proanthocyanidins of C. Read More

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Characteristics of experimental resin-modified glass-ionomer cements, containing alternate monomers to HEMA.

Dent Mater 2021 Aug 27. Epub 2021 Aug 27.

Centre for Oral Bioengineering, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, E1 4NS, United Kingdom.

Objective: Resin-modified glass ionomer cements (RMGICs) present several advantages (e.g. fluoride release), but their reported cytotoxicity has been associated with hydroxyethyl methacrylate (HEMA) monomer release. Read More

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A two-step resin based approach to reveal survivin-selective fluorescent probes.

RSC Chem Biol 2021 Feb 27;2(1):181-186. Epub 2020 Nov 27.

Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona Tucson AZ 85721 USA

The identification of modulators for proteins without assayable biochemical activity remains a challenge in chemical biology. The presented approach adapts a high-throughput fluorescence binding assay and functional chromatography, two protein-resin technologies, enabling the discovery and isolation of fluorescent natural product probes that target proteins independently of biochemical function. The resulting probes also suggest targetable pockets for lead discovery. Read More

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February 2021

Direct and indirect monomer elution from an RBC product family.

Dent Mater 2021 Aug 25. Epub 2021 Aug 25.

Department of Conservative Dentistry and Periodontology, University Hospital, LMU Munich Ludwig-Maximilians-University of Munich, Goethestr. 70, 80336 Munich, Germany.

Objective: To develop a model for quantitative comparison of elutable substances by direct elution from resin-bonded composite (RBC) test specimens versus indirect elutability of substances from RBC-restored teeth. Furthermore, it was to be investigated whether the different composites of the Tetric® RBC product family release different types and amounts of substances.

Methods: Four different composite materials from the Tetric® product family were studied. Read More

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Chemical Profile, Antioxidant Properties and Antimicrobial Activities of Malaysian Bee Bread.

Molecules 2021 Aug 15;26(16). Epub 2021 Aug 15.

Nutrition and Dietetics Program, School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.

The aim of the study was to determine the chemical profile, antioxidant properties and antimicrobial activities of bee bread from Malaysia. The pH, presence of phytochemicals, antioxidant properties, total phenolic content (TPC) and total flavonoid content (TFC), as well as antimicrobial activities, were assessed. Results revealed a decrease in the pH of bee bread water extract (BBW) relative to bee bread ethanolic extract (BBE) and bee bread hot water extract (BBH). Read More

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Cytotoxic Evaluation and Determination of Organic and Inorganic Eluates from Restorative Materials.

Molecules 2021 Aug 13;26(16). Epub 2021 Aug 13.

Division of Dental Tissues' Pathology and Therapeutics (Basic Dental Sciences, Endodontology and Operative Dentistry), School of Dentistry, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

Over the last years, diverse commercial resin-based composites have dominated as dental filling materials. The purpose of the present study was to determine organic and inorganic eluates from five restorative materials using GC/MS and ICP-OES and to compare the effect on cell survival of human gingival fibroblasts of a conventional and a bioactive resin. Five commercially available restorative materials were employed for this study: Activa Bioactive Restorative, ENA HRi, Enamel plus HRi Biofunction, Fuji II LC Capsule, and Fuji IX Capsule. Read More

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Medical Plant Extract Purification from Cadmium(II) Using Modified Thermoplastic Starch and Ion Exchangers.

Materials (Basel) 2021 Aug 22;14(16). Epub 2021 Aug 22.

Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Chaowang Road 18, Hangzhou 310014, China.

Pure compounds extracted and purified from medical plants are crucial for preparation of the herbal products applied in many countries as drugs for the treatment of diseases all over the world. Such products should be free from toxic heavy metals; therefore, their elimination or removal in all steps of production is very important. Hence, the purpose of this paper was purification of an extract obtained from et Migo and cadmium removal using thermoplastic starch (S1), modified TPS with poly (butylene succinate); 25% of TPS + 75% PBS (S2); 50% of TPS + 50% PLA (S3); and 50% of TPS + 50% PLA with 5% of hemp fibers (S4), as well as ion exchangers of different types, e. Read More

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Advances in Purification of SARS-CoV-2 Spike Ectodomain Protein Using High-Throughput Screening and Non-Affinity Methods.

Res Sq 2021 Aug 20. Epub 2021 Aug 20.

The spike (S) glycoprotein of the pandemic virus, SARS-CoV-2, is a critically important target of vaccine design and therapeutic development. A high-yield, scalable, cGMP-compliant downstream process for the stabilized, soluble, native-like S protein ectodomain is necessary to meet the extensive material requirements for ongoing research and development. As of June 2021, S proteins have exclusively been purified using difficult-to-scale, low-yield methodologies such as affinity and size-exclusion chromatography. Read More

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Evaluation of Adhesive Bond Strength, and the Sustained Release of Fluoride by Chitosan-infused Resin-modified Glass Ionomer Cement: An Study.

Int J Clin Pediatr Dent 2021 Mar-Apr;14(2):254-257

Department of Pediatric and Preventive Dentistry, Bharati Vidyapeeth Dental College and Hospital, Pune, Maharashtra, India.

Aim And Objective: To evaluate the adhesive bond strength, and sustained release of fluoride in chitosan (CH)-infused RMGIC.

Materials And Methods: Twenty caries-free human permanent premolar teeth, extracted for orthodontic purposes, were cleaned and stored in thymol solution. The crown of each tooth was cut into two halves and RMGIC ( = 10) and CH-infused RMGIC ( = 10) was placed between the two halves of the crown. Read More

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Comparison of Protein A affinity resins for twin-column continuous capture processes: Process performance and resin characteristics.

J Chromatogr A 2021 Sep 9;1654:462454. Epub 2021 Aug 9.

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address:

Continuous chromatography is a promising technology for downstream processing of biopharmaceuticals. The operation of continuous processes is significantly different to batch-mode chromatography and needs comprehensive evaluation. In this work, the performances of four Protein A affinity resins were studied systematically for twin-column continuous capture processes. Read More

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September 2021

Selective ESI-MS detection of carbonyl containing compounds by aminooxyacetic acid immobilized on a resin.

Anal Chim Acta 2021 Sep 16;1176:338767. Epub 2021 Jun 16.

Faculty of Chemistry, University of Wrocław, Joliot-Curie 14, 50-383, Wrocław, Poland.

There are numerous examples of bioactive compounds containing carbonyl groups including modified proteins with oxidation of side chain of amino acid residues to aldehyde/ketone groups which are frequently considered as markers of oxidative stress. The carbonyl unit can be also distinguished as a substructure in many illegal drugs including anabolic steroids as well as cations derivatives. Based on chemoselective formation of oximes by solid phase immobilized hydroxylamine derivatives we proposed the protocol for derivatization and selective detection of carbonylated compounds in human serum albumin hydrolysate as a complex peptide mixture and of testosterone in urine samples. Read More

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September 2021

Exploration of fiber-based cation exchange adsorbents for the removal of monoclonal antibody aggregates.

J Chromatogr A 2021 Sep 8;1654:462451. Epub 2021 Aug 8.

Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany. Electronic address:

Cation exchange chromatography (CEX) is a widely used technique for the removal of monoclonal antibody (mAb) aggregates. At present, resins are mainly used for this purpose, as convective types of adsorbents such as membrane adsorbers (MAs) have often not demonstrated overall comparable performance for this particular application. Fiber-based adsorbents can overcome the current limitations of MAs with respect to permeability, binding capacity, and adsorbent cost, and could therefore be a viable alternative to resins for the removal of mAb aggregates. Read More

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September 2021

[Analysis of spectrum-activity relationship among antioxidant parts of Lycii Fructus using three different chemometrics methods].

Zhongguo Zhong Yao Za Zhi 2021 Jul;46(13):3377-3387

School of Chemistry and Chemical Engineering,North Minzu University Yinchuan 750021,China Key Laboratory of Chemical Engineering and Technology,State Ethnic Affairs Commission Yinchuan 750021,China.

The chemical components of Lycii Fructus were analyzed by liquid chromatography( LC) and mass spectrometry( MS for the establishment of spectrum-activity relationship,on the basis of which its antioxidant active ingredients were determined. In this experiment,Lycii Fructus was extracted with different solvents and then separated into 80 samples by macroporous adsorption resin and reversed-phase chromatography,respectively. The antioxidant components were enriched into 11 samples and their scavenging abilities against DPPH free radical and ferric ion reducing antioxidant power( FRAP) were significantly stronger than those before the treatment( P<0. Read More

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Phenolic extract of Libidibia ferrea inhibits dentin endogenous enzymatic activity depending on the adhesive system strategy.

Microsc Res Tech 2021 Aug 12. Epub 2021 Aug 12.

Faculdade São Leopoldo Mandic, Restorative Dentistry and Dental Materials Division, Campinas, SP, Brazil.

This study evaluated the influence of Libidibia ferrea (Lf) extract used as dentin pretreatment on the resin-dentin bond strength stability and dentin endogenous enzymatic activity. The phytochemical profile (PP) of the Lf extract was evaluated by liquid chromatography; particle size, polydispersity index (PdI), and zeta potential (ZP) were evaluated by dynamic light scattering. The tested groups were ER-Scotchbond Universal (SBU) in the etch-and-rinse (ER) mode; ERLf-SBU in the ER mode + Lf after etching; SE- SBU in the self-etch (SE) mode; and LfSE-Lf before SBU in the SE mode. Read More

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Spectroscopy NIR and MIR toward predicting simultaneous phenolic contents and antioxidant in red propolis by multivariate analysis.

Food Chem 2022 Jan 2;367:130744. Epub 2021 Aug 2.

Departamento de Tecnologia Rural e Animal, Universidade Estadual do Sudoeste da Bahia, Praça Primavera - 40, Bairro Primavera, 45700-000 Itapetinga, BA, Brazil. Electronic address:

Conventional methods for determining phenolics and their bioactive properties are expensive, time-consuming, and laborious. This hinders the quality control of red propolis, recognized for having different types of phenolic constituents with different bioactive properties, for example, its antioxidant properties. In this sense, we present a new application of near and medium infrared spectroscopy to predict phenolic constituents, total flavonoids, gallic acid, kaempferol, pyrocatechin, quercetin, and different antioxidant tests (DPPH radical, reducing power and protection of the β-carotene: linoleic acid system) of red propolis using chemometry. Read More

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January 2022

Expression and purification of recombinant alpha-toxin AnCra1 from the scorpion Androctonus crassicauda and its functional characterization on mammalian sodium channels.

Mol Biol Rep 2021 Sep 11;48(9):6303-6312. Epub 2021 Aug 11.

Biotechnology Research Center, Islamic Azad University, Shahrekord, Iran.

Background: Alpha-scorpion toxins with long-chain peptide and four disulfide bonds represent diverse pharmacological profiles for various subtypes of voltage-gated sodium channels. Obtaining the natural toxins are difficult and time-consuming process, which represents the major difficulty to interpreting analysis of their structural and functional properties.

Methods And Results: This study describes the toxin peptide and plasmid construct containing the gene coding for mammalian toxin AnCra1 from the scorpion Androctonus crassicauda venom. Read More

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September 2021

A single column separation method for barium isotope analysis of geologic and hydrologic materials with complex matrices.

Geochem Trans 2021 Aug 11;22(1). Epub 2021 Aug 11.

National Energy Technology Laboratory, 626 Cochrans Mill Road, P.O. Box 10940, Pittsburgh, PA, 15236-0940, USA.

The increasing significance of barium (Ba) in environmental and geologic research in recent years has led to interest in the application of the Ba isotopic composition as a tracer for natural materials with complex matrices. Most Ba isotope measurement techniques require separation of Ba from the rest of sample prior to analysis. This paper presents a method using readily available materials and disposable columns that effectively separates Ba from a range of geologic and hydrologic materials, including carbonate minerals, silicate rocks, barite, river water, and fluids with high total dissolved solids and organic content such as oil and gas brines, rapidly and without need for an additional cleanup column. Read More

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Water, electricity and ion exchange; how Hamish Small sustained the evolution of ion chromatography.

John M Riviello

Heliyon 2021 Jul 6;7(7):e07495. Epub 2021 Jul 6.

Thermo Fisher Scientific, 1228 Titan Way, Sunnyvale, CA 94086, USA.

Since the development of synthetic ion exchange resins in 1935, the electrochemical properties of these unique materials have intrigued researchers. In 1951, Hamish Small was introduced to synthetic ion exchange resin when he studied the electromigration of cations in these new materials. In 1955, he began working in the ion exchange division of the Physical Research Laboratory at Dow Chemical. Read More

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