18,625 results match your criteria chromatography processing


Pungent and volatile constituents of dried Australian ginger.

Curr Res Food Sci 2021 4;4:612-618. Epub 2021 Sep 4.

School of Health, Medical & Applied Sciences, CQUniversity Australia, Bruce Hwy, North Rockhampton, Qld, 4701, Australia.

Ginger is well known for its pungent flavour and health-benefitting properties, both of which are imparted by various gingerol derivatives and other volatile constituents. Although there has been a considerable amount of research into the chemical constituents found in fresh ginger, there is little information available on the quality of Australian-grown dried ginger, particularly that intended for processing purposes. Here, we investigate differences in the chemical composition of three samples of processing-grade ginger, ranging from very poor to good quality. Read More

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September 2021

Catalytic pyrolysis of linear low-density polyethylene using recycled coal ash: Kinetic study and environmental evaluation.

Korean J Chem Eng 2021 Sep 10:1-12. Epub 2021 Sep 10.

New Materials Institute, University of Nottingham Ningbo China, Ningbo, 315042 P. R. China.

Catalytic pyrolysis offers a sustainable route to convert plastic wastes into fuel. We investigated the catalytic performance of coal ash (fly and bottom ash) at blending ratio of 5 wt%, and 15 wt% during pyrolysis of linear low-density polyethylene (LLDPE). The influence on activation energy and oil was characterized via thermogravimetric analyzer (TGA) and gas chromatography-mass spectrometry (GC-MS). Read More

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September 2021

Synthesis and evaluation of fosfomycin group end-capped packing materials for hydrophilic interaction liquid chromatography.

J Chromatogr A 2021 Sep 4;1656:462529. Epub 2021 Sep 4.

CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China. Electronic address:

Hydrophilic interaction liquid chromatography (HILIC) plays an important role in the analysis of compounds having high polarity. In this study, fosfomycin (F) was chosen as a new end-capping reagent, owing to the facile hydrolysis reaction of its epoxy group. Zirconia coated silica (ZrO/SiO) materials having good chemical and physical stability were prepared. Read More

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September 2021

Model Studies on the Reaction Products Formed at Roasting Temperatures from either Catechin or Tea Powder in the Presence of Glucose.

J Agric Food Chem 2021 Sep 14. Epub 2021 Sep 14.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.

During tea processing, roasting significantly affects the transformation pathway of catechins. When (-)-epigallocatechin gallate (EGCG) and glucose were roasted at different pH values, the degree of degradation and isomerization of EGCG was the lowest at pH 7 and the highest at pH 8. Thirty-five products were found in the model reaction of EGCG and glucose under high temperatures, of which four EGCG-glucose adducts were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR). Read More

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September 2021

Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody-Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry.

Anal Chem 2021 Sep 14. Epub 2021 Sep 14.

Spectroswiss, 1015 Lausanne, Switzerland.

The therapeutic efficacy and pharmacokinetics of antibody-drug conjugates (ADCs) in general, and antibody-oligonucleotide conjugates (AOCs) in particular, depend on the drug-to-antibody ratio (DAR) distribution and average value. The DAR is considered a critical quality attribute, and information pertaining to it needs to be gathered during ADC/AOC development, production, and storage. However, because of the high structural complexity of ADC/AOC samples, particularly in the initial drug-development stages, the application of the current state-of-the-art mass spectrometric approaches can be limited for DAR analysis. Read More

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September 2021

A Native Mass Spectrometry-Based Assay for Rapid Assessment of the Empty:Full Capsid Ratio in Adeno-Associated Virus Gene Therapy Products.

Anal Chem 2021 Sep 14. Epub 2021 Sep 14.

National Institute for Bioprocessing Research and Training (NIBRT), Foster Avenue, Blackrock, Dublin A94 X099, Ireland.

Adeno-associated virus (AAV)-based gene therapy is a rapidly developing field, requiring analytical methods for detailed product characterization. One important quality attribute of AAV products that requires monitoring is the amount of residual empty capsids following downstream processing. Traditionally, empty and full particles are quantified via analytical ultracentrifugation as well as anion exchange chromatography using ultraviolet or fluorescence detection. Read More

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September 2021

Novel sampling strategy for alive animal volatolome extraction combined with GC-MS based untargeted metabolomics: Identifying mouse pup pheromones.

Talanta 2021 Dec 4;235:122786. Epub 2021 Aug 4.

Laboratory of Functional Neuroanatomy (Unitat Mixta NeuroFun-UV-UJI), Predepartamental Unit of Medicine, Universitat Jaume I, Av. Sos Baynat S/N, 12071, Castellón de la Plana, Spain.

In this study, we identify 11 mouse pup volatiles putatively involved in maternal care induction in adult females. For this purpose, we have adapted the dynamic headspace methodology to extract the volatolome of whole alive animals. Untargeted metabolomic methodology was used to compare the volatolome of neonatal (4-6 days) with elder pups until the age of weaning (21-23 days old). Read More

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December 2021

Production of full-length SARS-CoV-2 nucleocapsid protein from Escherichia coli optimized by native hydrophobic interaction chromatography hyphenated to multi-angle light scattering detection.

Talanta 2021 Dec 16;235:122691. Epub 2021 Jul 16.

Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), 1190 Vienna, Austria; acib - Austrian Centre of Industrial Biotechnology, 1190, Vienna, Austria.

The nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for several steps of the viral life cycle, and is abundantly expressed during infection, making it an ideal diagnostic target protein. This protein has a strong tendency for dimerization and interaction with nucleic acids. For the first time, high titers of NP were expressed in E. Read More

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December 2021

The biochemical fate of Ag ions in Staphylococcus aureus, Escherichia coli, and biological media.

J Inorg Biochem 2021 Sep 3;225:111598. Epub 2021 Sep 3.

Department of Chemistry, The University of Adelaide, South Australia 5005, Australia,. Electronic address:

Silver is commonly included in a range of household and medical items to provide bactericidal action. Despite this, the chemical fate of the metal in both mammalian and bacterial systems remains poorly understood. Here, we applied a metallomics approach using X-ray absorption spectroscopy (XAS) and size-exclusion chromatography hyphenated with inductively coupled plasma mass spectrometry (SEC-ICP-MS) to advance our understanding of the biochemical fate of silver ions in bacterial culture and cells, and the chemistry associated with these interactions. Read More

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September 2021

Histological evaluation of sex differentiation and early sex identification in hatchery-produced greater amberjack (Seriola dumerili) reared in sea cages.

Fish Physiol Biochem 2021 Sep 13. Epub 2021 Sep 13.

Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Center for Marine Research, P.O. Box 2214, 71003, Heraklion, Crete, Greece.

The histological process of gonadal differentiation, together with the endocrine changes of sex steroid hormones and some of their precursors, was studied in hatchery-produced greater amberjack Seriola dumerili from 101 until 408 days post-hatching (dph), with samplings conducted every 50 days. Histological processing showed that sex differentiation began at 101 dph with the formation of the ovarian cavity in females, while the presumptive males did not yet contain any germ cells in their gonad. At 150 dph, we observed the first germ cells in the developing testes. Read More

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September 2021

Screening and heterologous expression of flavone synthase and flavonol synthase to catalyze hesperetin to diosmetin.

Biotechnol Lett 2021 Sep 12. Epub 2021 Sep 12.

Longping Branch Graduate School of Hunan University, Changsha, 410125, People's Republic of China.

Objectives: In this study, 44 flavone synthases (FNS) and flavonol synthases (FLS) from different origins were collected. The instability index and conserved domain of the enzymes were analyzed through bioinformatics analysis, the results of which allowed us to screen suitable enzymes for constructing recombinant Escherichia coli. Defective enzymes were selected as controls. Read More

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September 2021

Dietary Protein From Different Sources Exerted a Great Impact on Lipid Metabolism and Mitochondrial Oxidative Phosphorylation in Rat Liver.

Front Nutr 2021 26;8:719144. Epub 2021 Aug 26.

Key Laboratory of Meat Processing and Quality Control MOE, Nanjing Agricultural University, Nanjing, China.

Associations between meat diets and human health have been widely considered. In this study, we focused on long-term effects of different sources of meat protein on liver metabolic enzymes. For 90 days, rats were fed with semisynthetic diets that differed only with protein source. Read More

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Quantitative Determination of 15 Active Components in with UHPLC-PDA and GC-MS.

J Anal Methods Chem 2021 1;2021:6333989. Epub 2021 Sep 1.

School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563006, China.

In this study, a method using ultrahigh-performance liquid chromatography with photodiode array (UHPLC-PDA) was established and validated for the simultaneous quantification of 10 active components, including eight macamides and two glucosinolates, in (maca). A gas chromatographic mass spectroscopy (GC-MS) method was used to determine the levels of three benzyl isothiocyanates and two sterols in maca. Liquid chromatographic separation was achieved on a Waters Acquity UHPLC HSS T3 column (2. Read More

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September 2021

Untargeted metabolomics coupled with chemometric analysis deducing robust markers for discrimination of processing procedures: wine-processed Angelica sinensis as a case study.

J Sep Sci 2021 Sep 12. Epub 2021 Sep 12.

School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, 210029, China.

Wine-processed Angelica Sinensis is a widely used Chinese medicinal decoction piece in China. However, there are hardly any robust markers indicating the processing procedure of wine-processed Angelica Sinensis, including the amount of rice wine and processing degree. A strategy integrating untargeted metabolomics and chemometric analysis for deducing robust markers was provided and applied to the discrimination of processing procedure. Read More

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September 2021

Analysis of flavor formation during production of Dezhou braised chicken using headspace-gas chromatography-ion mobility spec-trometry (HS-GC-IMS).

Food Chem 2021 Sep 1;370:130989. Epub 2021 Sep 1.

Shandong Hanon Scientific Instrument Co., Ltd., China.

In order to help the poultry industry to generate higher quality products, the headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) technique was used to identify volatile substances formed during the processing of Dezhou braised chicken (DBC). A total of 37 volatile substances including aldehydes, alcohols, ketones, esters, terpenoids, furans and pyrazines were identified during DBC processing across seven sampling stages. The analyses identified 2-ethylhexanol as a key flavor chemical within the chicken carcasses, and found that ethyl acetate, 1-hexanol, 4-methyl-2-pentanone and 1-pentanol were mainly produced during the deep-frying stage of processing. Read More

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September 2021

Quantification and in silico analysis of taste dipeptides generated during dry-cured ham processing.

Food Chem 2021 Aug 30;370:130977. Epub 2021 Aug 30.

Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, 46980 Paterna, Valencia, Spain. Electronic address:

Small peptides such as dipeptides contribute to a great extent to the characteristic taste of dry-cured hams. In this study, hydrophilic interaction liquid chromatography (HILIC) combined to tandem mass spectrometry was used to separate, identify, and quantify seven dipeptides in dry-cured hams sampled at different processing times (6, 12, 18, and 24 months). Results showed an increased concentration of dipeptides DA, DG, EE, ES, and EV with the length of processing, obtaining values up to 23 μg/g of dry-cured ham, which suggests an intense action of muscle enzymes dipeptidyl peptidases during the process. Read More

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Mycotoxigenic and phylogenetic perspective to the yeasts and filamentous moulds in mould-matured Turkish cheese.

Int J Food Microbiol 2021 Sep 6;357:109385. Epub 2021 Sep 6.

Department of Food Processing, Bor Vocational School, Niğde Omer Halisdemir University, Nigde, Turkey.

This study was conducted to determine the diversity of yeasts and filamentous moulds in mould-matured cheese (MMC) consumed in Turkey. Overall, 120 samples were collected from 12 different geographical locations between March 2016 and April 2017. The morphological observation was applied in combination with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and molecular analyses to determine yeasts and filamentous moulds in the cheeses. Read More

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September 2021

Untargeted and targeted metabolomics reveal changes in the chemical constituents of instant dark tea during liquid-state fermentation by Eurotium cristatum.

Food Res Int 2021 Oct 21;148:110623. Epub 2021 Jul 21.

State Key Laboratory of Tea Plant Biology and Utilization, Key Laboratory of Food Nutrition and Safety, Anhui Engineering Laboratory for Agro-products Processing, School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, Anhui, China. Electronic address:

Instant green tea powder was used as raw material to prepare an instant dark tea via liquid-state fermentation by Eurotium cristatum. To understand how the chemical constituents present in fermented green tea develop during fermentation, samples were collected on different days during fermentation for qualitative analyses by ultra-performance liquid chromatography-Q Exactive Orbitrap/Mass spectrometry. Untargeted metabolomics analyses revealed that the levels of original secondary metabolites in the instant green tea changed significantly from day 3 to day 5 during fermentation. Read More

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October 2021

Quantitative changes in monosaccharides of Keemun black tea and qualitative analysis of theaflavins-glucose adducts during processing.

Food Res Int 2021 Oct 3;148:110588. Epub 2021 Jul 3.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China; International Joint Laboratory on Tea Chemistry and Health Effects of Ministry of Education, Anhui Agricultural University, Hefei 230036, China. Electronic address:

Monosaccharides of Keemun black tea were quantitatively determined by high performance liquid chromatography coupled with 3-methyl-1-phenyl-2-pyrazolin-5-one (PMP) pre-column derivatization. The methodology of developed analytical method was established with good linearity, recovery, repeatability and precision. The quantitative results showed that D-mannose, D-glucuronic acid, D-glucose, D-galactose and L-arabinose were detected in Keemun black tea samples. Read More

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October 2021

A high cell density perfusion process for Modified Vaccinia virus Ankara production: process integration with inline DNA digestion and cost analysis.

Biotechnol Bioeng 2021 Sep 10. Epub 2021 Sep 10.

Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, 39106, Magdeburg, Germany.

By integrating continuous cell cultures with continuous purification methods, process yields and product quality attributes were improved over the last 10 years for recombinant protein production. However, for the production of viral vectors such as Modified Vaccinia virus Ankara (MVA), no such studies have been reported although there is an increasing need to meet the requirements for a rising number of clinical trials against infectious or neoplastic diseases. Here, we present for the first time a scalable suspension cell (AGE1. Read More

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September 2021

[Extraction and isolation of histones from paraffin-embedded tissues and quantitative analysis of post-translational modifications].

Se Pu 2021 Oct;39(10):1094-1101

School of Basic Medical Sciences, Tianjin Medical University, Tianjin Key Lab for Medical Epigenetics, Tianjin 300070, China.

Histone post-translational modifications (HPTMs) have been believed to play crucial roles in the regulation of gene transcription. Thus, aberrant modification of histone can contribute to the occurrence and development of diseases such as tumors. To date, formalin fixed paraffin-embedded (FFPE) clinical tissues are believed to be one of the most valuable sample resources in the study of human diseases. Read More

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October 2021

[Recent advances in glycopeptide enrichment and mass spectrometry data interpretation approaches for glycoproteomics analyses].

Se Pu 2021 Oct;39(10):1045-1054

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Protein glycosylation is one of the most important post-translational modifications (PTMs). The glycosylation is crucial in a variety of physiological and pathological processes that include protein stability, intracellular and intercellular signal transduction, hormone activation or inactivation, and immune regulation. Protein glycosylation is generated by complex biosynthetic pathways comprising hundreds of glycosyltransferases, glycosidases, transcriptional factors, transporters, and protein backbones. Read More

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October 2021

Model Composites Based on Poly(lactic acid) and Bioactive Glass Fillers for Bone Regeneration.

Polymers (Basel) 2021 Sep 3;13(17). Epub 2021 Sep 3.

CNRS, UMR 5223, Ingénierie des Matériaux Polymères, INSA Lyon, Université de Lyon, F-69621 Villeurbanne, France.

Poly(l-lactide-co-d,l-lactide) PDLA/45S5 Bioglass (BG) composites for medical devices were developed using an original approach based on a thermal treatment of BG prior to processing. The aim of the present work is to gain a fundamental understanding of the relationships between the morphology, processing conditions and final properties of these biomaterials. A rheological study was performed to evaluate and model the PDLA/BG degradation during processing. Read More

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September 2021

Hydrothermal Processing and In Vitro Simulated Human Digestion Affects the Bioaccessibility and Bioactivity of Phenolic Compounds in African Pumpkin () Leaves.

Molecules 2021 Aug 27;26(17). Epub 2021 Aug 27.

Department of Biotechnology and Food Technology, Faculty of Science, Doornfontein Campus, University of Johannesburg, P.O. Box 17011, Doornfontein, Johannesburg 2028, South Africa.

The African pumpkin () contains bioactive phenolic compounds that may assist in reducing oxidative stress in the human body. The leaves are mainly consumed after boiling in water for a specific time; this hydrothermal process and conditions of the gastrointestinal tract may affect the presence and bioactivity of phenolics either positively or negatively. In this study, the effects of hydrothermal processing (boiling) and in vitro simulated human digestion on the phenolic composition, bioaccessibility and bioactivity in African pumpkin were investigated in comparison with those of spinach (). Read More

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Traceability of VOCs in tire inner liner by chromatography-mass spectrometry.

Environ Sci Pollut Res Int 2021 Sep 9. Epub 2021 Sep 9.

The Key Laboratory of Beijing on Preparation and Processing of Novel Polymer Materials, Beijing University of Chemical Technology, Beijing, 100029, China.

Many parts of the vehicle cabin generate volatile organic compounds (VOCs), and some are hazardous and/or odorous to humans. In this study, VOCs in the inner liner of automobile spare tire, including raw rubbers and resins, were detected by gas chromatography-mass spectrometry (GC-MS) coupled with an extracting method of static headspace sampling (SHS). The results demonstrated that the sources of VOCs can be traced back to raw rubbers and resins: alkylphenol resins can release a large amount of 2,4,4-trimethyl-1-pentene and 2,2,4,6,6-pentamethyl-3-heptene; chlorobutyl rubber (CIIR) contained 3-methyl-pentane, and methyl-cyclopentane, and these VOCs are odorous. Read More

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September 2021

One-step synthesis of nitrogen-doped multi-emission carbon dots and their fluorescent sensing in HClO and cellular imaging.

Mikrochim Acta 2021 Sep 8;188(10):330. Epub 2021 Sep 8.

College of Chemistry, Beijing Normal University, Beijing, 100875, China.

Tunable multicolor carbon dots (CDs) with a quantum yield reach up to 35% were generated directly from rhodamine and urea via one-step hydrothermal approach and purified through silica gel column chromatography. Transmission electron microscopy images reveal that the as-prepared CDs possess a small size distribution below 10 nm with bright blue, green, and yellow color emission, designated as b-CDs, g-CDs, and y-CDs, respectively. The in-depth investigations reveal that the multicolor emission CDs with different fraction displays fluorescence emission wavelength ranges from 398 nm (b-CDs), 525 nm (g-CDs), to 553 nm (y-CDs) which could be well modulated by controlling the amount of heteroatom nitrogen especially amino nitrogen onto their surface structures. Read More

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September 2021

LipidQuant 1.0: automated data processing in lipid class separation-mass spectrometry quantitative workflows.

Bioinformatics 2021 Sep 8. Epub 2021 Sep 8.

Department of Analytical Chemistry, University of Pardubice, Faculty of Chemical Technology, Pardubice, Czech Republic.

Summary: We present the LipidQuant 1.0 tool for automated data processing workflows in lipidomic quantitation based on lipid class separation coupled with high-resolution mass spectrometry. Lipid class separation workflows, such as hydrophilic interaction liquid chromatography or supercritical fluid chromatography, should be preferred in lipidomic quantitation due to the coionization of lipid class internal standards with analytes from the same class. Read More

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September 2021

Quantitative Proteomic Analysis of Synovial Tissue Reveals That Upregulated OLFM4 Aggravates Inflammation in Rheumatoid Arthritis.

J Proteome Res 2021 Sep 9. Epub 2021 Sep 9.

Department of Joint Surgery, Xi'an Hong Hui Hospital, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710054, P. R. China.

Tandem mass tag (TMT)-coupled liquid chromatography coupled with tandem mass spectrometry is a powerful method to investigate synovial tissue protein profiles in patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Protein was isolated from synovial tissue samples of 22 patients and labeled with a TMT kit. Over 500 proteins were identified as the differential expression protein on comparing RA and OA synovial tissue, including 239 upregulated and 271 downregulated proteins. Read More

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September 2021

Novel Broccoli-Derived Peptides Hydrolyzed by Trypsin with Dual-Angiotensin I-Converting Enzymes and Dipeptidyl Peptidase-IV-Inhibitory Activities.

J Agric Food Chem 2021 Sep 8. Epub 2021 Sep 8.

Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, China.

Broccoli-derived peptides show beneficial metabolic effects, and it is necessary to examine their exact functional sequences. First, peptides from the trypsin hydrolysate of broccoli proteins were isolated and identified using column chromatography and quadrupole time-of-flight mass spectrometry. After that, their functions were verified by oral administration. Read More

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September 2021

Hyperbranched anion exchangers prepared from polyethylene polyamine modified polymeric substrates for ion chromatography.

J Chromatogr A 2021 Aug 28;1655:462508. Epub 2021 Aug 28.

Department of Chemistry, Zhejiang University, Xixi Campus, Hangzhou 310028, China. Electronic address:

High hydrophilic anion stationary phases play a crucial role in the separation behavior of ion chromatography. Herein, we report novel polymeric anion exchangers grafted with polyethylene polyamines, including ethylenediamine, diethylenetriamine, triethylenetetramine and tetraethylene pentaamine, via a facile epoxy-amine polymerization method. The anion exchangers were characterized by scanning electron microscopy, thermogravimetry, Fourier transform infrared spectrometry and elemental analysis. Read More

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