780 results match your criteria chlorin method

A biodegradable nano-photosensitizer with photoactivatable singlet oxygen generation for synergistic phototherapy.

J Mater Chem B 2021 Jun 14. Epub 2021 Jun 14.

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.

Photodynamic therapy (PDT) is a promising method for cancer therapy and also may initiate unexpected damages to normal cells and tissues. Herein, we develop a near-infrared (NIR) light-activatable nanophotosensitizer, which shows negligible phototoxicity before photoactivation to improve the specificity of PDT. The nanophotosensitizer is prepared by indocyanine green carboxylic (ICG), Chlorin e6 (Ce6), and biodegradable poly (lactic acid) (PLA) and poly (lactic-co-glycolic acid) (PLGA), and all these materials have been approved by the Food and Drug Administration. Read More

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Determination of Optical Properties and Photodynamic Threshold of Lung Tissue for Treatment Planning of In Vivo Lung Perfusion Assisted Photodynamic Therapy.

Photodiagnosis Photodyn Ther 2021 May 26;35:102353. Epub 2021 May 26.

University of Toronto, Faculty of Medicine, Department of Medical Biophysics, 101 College Street, Toronto M5G 1L7, Ontario, Canada; Princess Margaret Cancer Centre, University Health Network, 101 College St, Toronto M5G 2C4, Ontario, Canada. Electronic address:

Background: Isolated lung metastases in sarcoma and colorectal cancer patients are inadequately treated with current standard therapies. In Vivo Lung Perfusion, a novel platform, could overcome limitations to photodynamic therapy treatment volumes by using low cellular perfusate, removing blood, theoretically allowing greater light penetration. To develop personalized photodynamic therapy protocols requires in silico light propagation simulations based on optical properties and maximal permissible photodynamic threshold dose of lung tissue. Read More

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Nanobubbles Containing sPD-1 and Ce6 Mediate Combination Immunotherapy and Suppress Hepatocellular Carcinoma in Mice.

Int J Nanomedicine 2021 10;16:3241-3254. Epub 2021 May 10.

Medical College of China Three Gorges University, Yichang, Hubei, People's Republic of China.

Purpose: Immune checkpoint inhibitors (ICIs) and sonodynamic therapy (SDT) are types of immunotherapy. In order to combine soluble programmed cell death protein 1 (sPD-1)-mediated immune checkpoint therapy and chlorin e6 (Ce6)-assisted SDT, nanobubbles (NBs) were generated to simultaneously load sPD-1 and Ce6.

Materials And Methods: The sPD-1/Ce6-NBs, which were prepared by thin-film hydration and mechanical oscillation, had a stable physical condition, and delivered sPD-1 and Ce6 in a targeted manner. Read More

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Chlorin e6 conjugated chitosan as an efficient photoantimicrobial agent.

Int J Biol Macromol 2021 May 14;183:1309-1316. Epub 2021 May 14.

State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China; International Joint Laboratory on Food Safety, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China. Electronic address:

The development of antibacterial agents with high bacteria-binding capability and antibacterial efficiency is highly desirable. Herein, cationic polysaccharide chitosan (CS) was combined with photosensitizer Chlorin e6 (Ce6) to construct a novel photodynamic antibacterial agent (CS-Ce6 conjugates) for combating gram-positive bacteria Staphylococcus aureus (S. aureus) and gram-negative bacteria Escherichia coli (E. Read More

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One-pot synthesis of a self-reinforcing cascade bioreactor for combined photodynamic/chemodynamic/starvation therapy.

J Colloid Interface Sci 2021 Oct 2;599:543-555. Epub 2021 Apr 2.

Department of Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region. Electronic address:

The combination of photodynamic therapy (PDT) and chemodynamic therapy (CDT) have attracted a great deal of interest, but tumor hypoxia and glutathione (GSH) overproduction still limit their further applications. Herein, an intelligent reactive oxygen species (ROS) nanogenerator Ce6/[email protected]/[email protected] (denoted as CGZPM; Ce6, GOx, ZIF-8, PDA, MnO are chlorin e6, glucose oxidase, zeolitic imidazolate framework-8, polydopamine and manganese dioxide respectively) with O-generating and GSH-/glucose-depleting abilities was constructed by a facile and green one-pot method. After intake by tumor cells, the outer MnO was rapidly degraded by the acidic pH, and the overexpression of hydrogen peroxide (HO) and GSH with abundant Mn and O produced would eventually achieve multifunctionality. Read More

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October 2021

Meso-Biphenyl-Linked, Near- and Far-Infrared Emitting, Chlorin and Bacteriochlorin Dimers: Synthesis, Excitation Transfer, and Singlet Oxygen Production.

Chempluschem 2021 Apr 1;86(4):674-680. Epub 2021 Apr 1.

Department of Chemistry, University of North Texas, 1155 Union Circle, #305070, Denton, TX 76203-5017, USA.

A series of meso-biphenyl linked chlorin and bacteriochlorin dimers, derived from naturally occurring chlorophyll (Chl-a) and bacteriochlorophyll (BChl-a) were synthesized in 32 % to 44 % yields and characterized, as photosynthetic antenna mimics, and a new class of singlet oxygen producing agents. The dimers are characterized by absorption, fluorescence, electrochemical, spectroelectrochemical and computational methods to evaluate their physico-chemical properties, and to identify ground and excited state interactions. Evidence of excited energy exchange among the chromophores in the dimer is derived from femtosecond transient absorption spectral studies. Read More

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Ultrathin 2D Copper(I) 1,2,4-Triazolate Coordination Polymer Nanosheets for Efficient and Selective Gene Silencing and Photodynamic Therapy.

Adv Mater 2021 May 2;33(18):e2100849. Epub 2021 Apr 2.

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.

Gene silencing holds promise for cancer therapeutics because of its potential to inhibit genes involved in tumor development. However, gene silencing is still restricted by its limited efficacy and safety. Nanoscale coordination polymers (CPs) emerge as promising nanocarriers for gene delivery, but their responsiveness and potential therapeutic properties have rarely been explored simultaneously. Read More

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An effective method to generate controllable levels of ROS for the enhancement of HUVEC proliferation using a chlorin e6-immobilized PET film as a photo-functional biomaterial.

Regen Biomater 2021 Mar 13;8(2):rbab005. Epub 2021 Mar 13.

Cellbiocontrol Laboratory, Department of Medical Engineering.

Reactive oxygen species (ROS) are byproducts of cellular metabolism; they play a significant role as secondary messengers in cell signaling. In cells, high concentrations of ROS induce apoptosis, senescence, and contact inhibition, while low concentrations of ROS result in angiogenesis, proliferation, and cytoskeleton remodeling. Thus, controlling ROS generation is an important factor in cell biology. Read More

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Efficacy of root canal treatment in c-shaped canals with adjunctive photodynamic therapy using micro-CT.

Photodiagnosis Photodyn Ther 2021 Mar 15;34:102257. Epub 2021 Mar 15.

Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, P. O. Box: 10219, Riyadh, 11433, Saudi Arabia.

Aim: To investigate the efficacy of conventional root canal treatment (cRCT) with adjunctive photodynamic therapy (aPDT) against microbial biofilms within infected c-shaped root canals.

Methods: In this in vitro report, the inoculation of 20 freshly extracted human mandibular molar teeth having c-shaped root canal configuration was performed with E. faecalis and P. Read More

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Bioorthogonal Pretargeting Strategy for Anchoring Activatable Photosensitizers on Plasma Membranes for Effective Photodynamic Therapy.

ACS Appl Mater Interfaces 2021 Mar 17;13(12):14004-14014. Epub 2021 Mar 17.

Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou 510180, P.R. China.

Developing novel activatable photosensitizers with excellent plasma membrane targeting ability is urgently needed for smart photodynamic therapy (PDT). Herein, a tumor acidity-activatable photosensitizer combined with a two-step bioorthogonal pretargeting strategy to anchor photosensitizers on the plasma membrane for effective PDT is developed. Briefly, artificial receptors are first anchored on the cell plasma membrane using cell-labeling agents () the enhanced permeability and retention effect to achieve the tumor cell labeling. Read More

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Nanosonosensitizer-Augmented Sonodynamic Therapy Combined with Checkpoint Blockade for Cancer Immunotherapy.

Int J Nanomedicine 2021 5;16:1889-1899. Epub 2021 Mar 5.

Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Zhongshan Hospital Xiamen University, Xiamen, 361004, People's Republic of China.

Introduction: Sonodynamic therapy (SDT) has good targeting and non-invasive advantages in the treatment of solid cancers, and checkpoint blockade immunotherapy is also a promising treatment to cure cancer. However, their antitumor effects are not sufficient due to some inherent factors. Some studies that combined SDT with immunotherapy or nanoparticles have managed to enhance its efficiency to treat cancers. Read More

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[Cytotoxic effect of photodynamic liposome gel combined with trastuzumab on drugresistant breast cancer cells ].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Feb;41(2):164-172

School of Pharmacy, Southern Medical University, Guangzhou 510515, China.

Objective: To evaluate the cytotoxic effect of photodynamic therapy (PDT) combined with targeted therapy using cross-linked liposomes and gels ([email protected]) loaded with photosensitizer Chlorin (Ce6) and the tumor-targeting drug Trastuzumab (Tmab) in drug-resistant HER2+ breast cancer cells.

Objective: Ce6-PC-Tmab liposomes were prepared using the thin-film hydration method. The general properties, encapsulation efficiency and near-infrared responsivity of the nanoparticles were evaluated. Read More

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February 2021

Efficacy of Photodynamic Anti-Microbial Chemotherapy for Acanthamoeba Keratitis In Vivo.

Lasers Surg Med 2021 Jul 8;53(5):695-702. Epub 2020 Dec 8.

Department of Virology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, 734-8551, Japan.

Background And Objectives: Acanthamoeba keratitis is a sight-threatening infectious disease that is difficult to treat. The aim of this study was to evaluate TONS504 (cationic chlorin derivative photosensitizer)-mediated photodynamic antimicrobial chemotherapy (PACT) in vivo as a potential treatment for Acanthamoeba keratitis.

Study Design/materials And Methods: Acanthamoeba keratitis was induced by soft contact lenses incubated with 1 × 10 /ml Acanthamoeba castellanii, which were placed over debrided corneas with temporary tarsorrhaphy. Read More

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Nucleolin-Targeted Ratiometric Fluorescent Carbon Dots with a Remarkably Large Emission Wavelength Shift for Precise Imaging of Cathepsin B in Living Cancer Cells.

Anal Chem 2021 03 15;93(8):4042-4050. Epub 2021 Feb 15.

State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

As one of the most promising biomarkers for numerous malignant tumors, accurate and reliable reporting of Cathepsin B (CTSB) activity is of great significance to achieve efficient diagnosis of cancers at an early stage and predicting metastasis. Here, we report a vigorous ratiometric fluorescent method integrating a cancer-targeting recognition moiety with a remarkably large emission wavelength shift into a single matrix to report CTSB activity sensitively and specifically. As a proof of concept, we synthesized amine-rich carbon quantum dots (CQDs) with a blue fluorescence, which offered an efficient scaffolding to covalently assemble the nucleolin-targeting recognition nucleic acid aptamer AS1411 and a CTSB-cleavable peptide substrate Gly-Arg-Arg-Gly-Lys-Gly-Gly-Cys-COOH that tethered with a near-infrared (NIR) fluorophore chlorin e6 (Ce6-GRRGKGGC, Ce6-Pep), enabling a cancer-targeting and CTSB stimulus-responsive ratiometric nanoprobe AS1411-Ce6-CQDs. Read More

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Integrating Near-Infrared Visual Fluorescence with a Photoelectrochemical Sensing System for Dual Readout Detection of Biomolecules.

Anal Chem 2021 02 5;93(7):3486-3492. Epub 2021 Feb 5.

Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China.

Compared with traditional visible light-driven fluorescence visualization (FV), near-infrared (NIR)-induced FV is an interesting and promising method, while photoelectrochemical (PEC) immunoassay sensing possesses the advantages of high sensitivity, low cost, and simple instrumentation. We combined PEC sensing with NIR-induced FV together and developed a dual readout sensing platform. In this protocol, based on the antibody-analyte (i. Read More

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February 2021

2D LDH-MoS clay nanosheets: synthesis, catalase-mimic capacity, and imaging-guided tumor photo-therapy.

J Nanobiotechnology 2021 Feb 3;19(1):36. Epub 2021 Feb 3.

Department of Gastroenterology, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai, 200433, People's Republic of China.

Owing to the hypoxia status of the tumor, the reactive oxygen species (ROS) production during photodynamic therapy (PDT) of the tumor is less efficient. Herein, a facile method which involves the synthesis of Mg-Mn-Al layered double hydroxides (LDH) clay with MoS doping in the surface and anionic layer space of LDH was presented, to integrate the photo-thermal effect of MoS and imaging and catalytic functions of Mg-Mn-Al LDH. The designed LDH-MoS (LMM) clay composite was further surface-coated with bovine serum albumin (BSA) to maintain the colloidal stability of LMM in physiological environment. Read More

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February 2021

Photodynamic theranostics of central lung cancer: Present state and future prospects.

Photodiagnosis Photodyn Ther 2021 Mar 30;33:102203. Epub 2021 Jan 30.

Department of Thoracic Oncology, Institute of Surgery and Emergency Medicine, Pavlov First State Medical University, Saint-Petersburg, Russia; Institute of Experimental Medicine, Almazov Federal Medical Research Center, Saint-Petersburg, Russia.

Endobronchial photodynamic therapy (PDT) in central lung cancer (CLC) shows feasibility even in late stage disease. Our experience with chlorin e6 based photosensitizers (PS), including in combination with medical cancer treatment, demonstrated regression of tumor lesions of the trachea and bronchi in 94 % of patients with central NSCLC. It is possible to increase the efficiency of the treatment and achieve its personalization by using fluorescent bronchoscopes, which provide fluorescence guided PDT - photodynamic theranostics (PT). Read More

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Photodynamic effect of chlorin e6 on cytoskeleton protein of human colon cancer SW480 cells.

Photodiagnosis Photodyn Ther 2021 Mar 30;33:102201. Epub 2021 Jan 30.

College of Public Health, Xinjiang Medical University, Urumqi 830000, China. Electronic address:

Background: Photodynamic therapy (PDT) is based on photochemical and photobiological reactions mediated by photosensitizers to achieve a killing effect on diseased cells. It is used in the treatment of malignant tumors, precancerous lesions and infections.

Objective: In order to provide theoretical data for further study of the mechanism of PDT for colorectal cancer, SW480 cells were treated with Ce6-PDT and effect of photodynamic therapy (Ce6-PDT) on cytoskeleton and E-cadherin protein were observed. Read More

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Chlorin e6-1,3-diphenylisobenzofuran polymer hybrid nanoparticles for singlet oxygen-detection photodynamic abaltion.

Methods Appl Fluoresc 2021 Feb 13;9(2):025003. Epub 2021 Feb 13.

Beijing Key Laboratory of Work Safety Intelligent Monitoring, School of Electronic Engineering, Beijing University of Posts and Telecommunications, Haidian District, Beijing, 100876, People's Republic of China.

A dual-functional nanosysterm is developed by means of Chlorin e6 (Ce6) as photosensitizer and 1,3-Diphenylisobenzofuran (DPBF) as fluorescent singlet oxygen (O) probe. Under 660 nm laser irradiation, Ce6 exhibites efficient O generation, and subsequently the production of O is assessed by the ratiometric fluorescence of PFO and DPBF under one-photon and two-photon excitation mode. The nanoparticles with excellent biocompatibility can be internalized into Hela cells and applied for tumor treatment. Read More

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February 2021

Chlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer.

Photodiagnosis Photodyn Ther 2021 Mar 23;33:102186. Epub 2021 Jan 23.

Laboratório de Nanossensores, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, UNIVAP, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo 12244-000, Brazil. Electronic address:

To develop a treatment modality for triple-negative breast cancer, we investigated the efficacy of a bifunctional theranostic nanoprobes (BN) during Photodynamic Therapy (PDT) on human breast carcinoma and normal human cells. The BN is a 21 nm gold nanoparticles functionalized with Chlorin e6 (Ce6) and Epidermal Growth Factor (EGF). Attachment to gold nanoparticle stabilizes Ce6 while EGF acts as a cancer cell targeting agent. Read More

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Immune Reprogramming Precision Photodynamic Therapy of Peritoneal Metastasis by Scalable Stem-Cell-Derived Extracellular Vesicles.

ACS Nano 2021 02 22;15(2):3251-3263. Epub 2021 Jan 22.

Laboratoire MSC Matière et Systèmes Complexes, CNRS UMR 7057, Université de Paris, 10 Rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13, France.

The dissemination of tumor metastasis in the peritoneal cavity, also called peritoneal metastasis (PM) or carcinomatosis, represents a late stage of gastrointestinal and gynecological cancer with very poor prognosis, even when cytoreductive surgery is effective, due to residual microscopic disease. Photodynamic therapy (PDT) in the management of peritoneal metastasis has been clinically limited by the low tumor selectivity of photosensitizers (PS) and important adverse effects. Here, we propose extracellular nanovesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) as the fourth generation of immune active PS vectors that are able to target peritoneal metastasis with superior selectivity, potentiate PDT cytotoxicity at the tumor site without affecting healthy tissues, modulate the tumor microenvironment of immunocompetent colorectal and ovarian carcinomatosis models, and promote an antitumor immune response. Read More

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February 2021

Investigating the photodynamic efficacy of chlorin e6 by millisecond pulses in metastatic melanoma cells.

Bioelectrochemistry 2021 Apr 29;138:107728. Epub 2020 Dec 29.

Department of Physical and Quantum Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology, Poland.

Melanoma is considered the most aggressive type of skin cancer, still without effective treatment. Thus, alternative therapeutic methods are still in demand, and electroporation-supported photodynamic therapy (EP-PDT) of cancer cells seems a promising approach. New developments in EP-PDT aim at enhanced tumor selectivity and biocompatibility by applying a second-generation photosensitizer, i. Read More

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Multifunctional nanoparticle PEG‑Ce6‑Gd for MRI‑guided photodynamic therapy.

Oncol Rep 2021 02 27;45(2):547-556. Epub 2020 Nov 27.

Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430071, P.R. China.

Gliomas are one of the most common types of primary brain tumors. Despite recent advances in the combination of surgery, radiotherapy, systemic therapy (chemotherapy, targeted therapy) and supportive therapy in the multimodal treatment of gliomas, the overall prognosis remains poor and the long‑term survival rate is low. Thus, it is crucial to develop a novel glioma management method. Read More

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February 2021

Increased photodynamic therapy sensitization in tumors using a nitric oxide-based nanoplatform with ATP-production blocking capability.

Theranostics 2021 1;11(4):1953-1969. Epub 2021 Jan 1.

Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, P. R. China.

Photodynamic therapy (PDT) efficacy in cancer cells is affected by sub-physiological hypoxia caused by dysregulated and "chaotic" tumor microvasculature. However, current traditional O-replenishing strategies are undergoing their own intrinsic deficiencies. In addition, resistance mechanisms activated during PDT also lead the present situation far from satisfactory. Read More

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January 2021

Chlorin e6 embedded in phospholipid nanoparticles equipped with specific peptides: Interaction with tumor cells with different aminopeptidase N expression.

Biomed Pharmacother 2021 Feb 25;134:111154. Epub 2020 Dec 25.

MNII Them. P.A. Herzen - Branch of FSBI «NIIRTS» Ministry of Health of Russia, 2nd Botkinsky Passage, 3, Moscow, 125284, Russia. Electronic address:

A promising direction in Biopharmaceuticals is the development of specific peptide-based systems to improve drug delivery. This approach may increase tumor specificity and drug penetration into the target cell. Similar systems have been designed for several antitumor drugs. Read More

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February 2021

Downregulation of Rac1/PAK1/LIMK1/cofilin signaling pathway in colon cancer SW620 cells treated with Chlorin e6 photodynamic therapy.

Photodiagnosis Photodyn Ther 2021 Mar 8;33:102143. Epub 2020 Dec 8.

School of Public Health, Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang Uygur Autonomous Region, 10760, China. Electronic address:

Background: Colorectal cancer is one of the most common gastrointestinal malignancies. Photodynamic therapy (PDT) is a novel and non-invasive treatment for tumors as PDT features small trauma, good applicability, andaccurate targeting. PDT may also be a potential treatment for colon cancer as itmay may induce suppressive effects on metastatic potential. Read More

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The Effect of Anti-Amoebic Agents and Ce6-PDT on Trophozoites and Cysts, In Vitro.

Transl Vis Sci Technol 2020 11 23;9(12):29. Epub 2020 Nov 23.

Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Aniridia Research, Saarland University, Homburg/Saar, Germany.

Purpose: The purpose of this study was to analyze the concentration-dependent effects of biguanides (polyhexamethylene biguanide [PHMB], chlorhexidine [CH]); diamidines (hexamidine-diisethionate [HD], propamidine-isethionate [PD], dibromopropamidine-diisethionate [DD]); natamycin (NM); miltefosine (MF); povidone iodine (PVPI), and chlorin e6 PDT on trophozoites and cysts, in vitro.

Methods: Strain 1BU was cultured in peptone-yeast extract-glucose medium. Trophozoites or cysts were cultured in PYG medium containing each agent at 100%, 50%, and 25% of maximum concentration for 2 hours. Read More

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November 2020

In situ oxygenating and 808 nm light-sensitized nanocomposite for multimodal imaging and mitochondria-assisted cancer therapy.

J Mater Chem B 2021 01 23;9(1):131-146. Epub 2020 Nov 23.

Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin, 150001, P. R. China.

The efficiency of photodynamic therapy (PDT) is severely constrained due to the innate hypoxic environment, besides the elevated level of glutathione (GSH). To get rid of the hypoxic environment and higher concentrations of GSH in the solid tumors, we propose an approach of oxygen self-sufficient multimodal imaging-guided nanocomposite CaO-MnO-UCNPs-Ce6/DOX (abbreviated as CaMn-NUC), in which CaO nanoparticles in the hydrophobic layer were seated on the hydrophilic MnO sheet and conjugated with chlorin e6 (Ce6) loaded upconversion nanoparticles (UCNPs-Ce6) via the click chemistry approach. CaMn-NUC was presented to overcome hypoxia and GSH-associated photodynamic resistance due to in situ oxygen generation and GSH reduction of MnO upon endocytosis, and a bulk amount of Mn ions generated in the process under acidic tumor environment acts as the MRI contrast agent. Read More

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January 2021

Carbon Nanocage/FeO/DNA-Based Magnetically Targeted Intracellular Imaging of Telomerase via Catalyzed Hairpin Assembly and Photodynamic-Photothermal Combination Therapy of Tumor Cells.

ACS Appl Mater Interfaces 2020 Nov 19. Epub 2020 Nov 19.

Jiangsu Key Laboratory of Brain Disease and Bioinformation, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China.

Human telomerase has been identified as a potential tumor biomarker for early cancer diagnosis and cancer progression monitoring. We construct a novel magnetic targeting carbon nanocage/FeO/DNA (CNC/FeO/DNA) nanoprobe for intracellular imaging of telomerase via the signal amplification strategy catalyzed hairpin assembly (CHA) and for photodynamic-photothermal therapy of tumor cells. Telomerase primer DNA, trigger DNA, hairpin DNA1 (H1), and hairpin DNA2 (H2) were adsorbed to the surface of CNC/FeO nanoparticles (CNC/FeO NPs), and the fluorescence of (chlorin e6) Ce6 was quenched by CNC/FeO NPs. Read More

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November 2020

Innate immune activation by conditioned medium of cancer cells following combined phototherapy with photosensitizer-loaded gold nanorods.

J Mater Chem B 2020 12 11;8(47):10812-10824. Epub 2020 Nov 11.

NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, University Hall, Tan Chin Tuan Wing, Level 04, #04-02, 21 Lower Kent Ridge, Singapore 119077, Singapore.

Nanoparticle-based phototherapy has evolved to include immunotherapy as an effective treatment combination for cancers through inducing anti-cancer immune activation leading to downstream adaptive responses and immune protection. However, most cancer phototherapy studies that claimed anti-cancer immunogenic effects often included exogenous immunostimulants to potentiate immune responses and did not clearly establish their effects on immune cells. In this study, we showed that combined photodynamic (PDT) and photothermal therapy (PTT) using gold nanorods (NRs) loaded with the photosensitizer chlorin e6 (Ce6) on endogenously formed mouse serum (MS) protein coronas (i. Read More

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December 2020