4,938 results match your criteria characterization precision


Quantitative characterization of the B cell receptor repertoires of human immunized with commercial rabies virus vaccine.

Hum Vaccin Immunother 2021 Aug;17(8):2538-2546

Laboratory for Diagnosis of Clinical Microbiology and Infection, Yuebei People's Hospital, Shantou University Medical College, Shaoguan, China.

Humoral immunity is crucial for an efficient host immune response against rabies virus (RABV) infection. But the B cell receptor (BCR) repertoire in human after RABV vaccine immunization remained unclear. To study the BCR repertoires in peripheral blood mononuclear cells (PBMCs) of human immunized with rabies virus vaccine. Read More

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Multi-elemental Characterization of Semolina Samples by Inductively Coupled Plasma-Tandem Mass Spectrometry (ICP-MS/MS).

Biol Trace Elem Res 2021 Sep 24. Epub 2021 Sep 24.

Department of Environmental Engineering, Eskisehir Technical University, Eskisehir, Turkey.

The precise characterization of the elemental composition of cereals and cereal products is becoming crucial to assess their nutritional values, geographical labeling, and also toxicological profile. Inductively coupled plasma-mass spectrometry (ICP-MS) has become popular for the elemental analysis of foods in recent years. Analytical performance of the inductively coupled plasma-tandem mass spectrometry (ICP-MS/MS) is improved with a reaction/collision cell that provides an interference-free analysis of most of the elements including phosphorus and sulfur. Read More

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September 2021

Identification and Characterization of a Germline Mutation in CARD11 From a Chinese Case of B Cell Expansion With NF-κB and T Cell Anergy.

Front Immunol 2021 7;12:676386. Epub 2021 Sep 7.

Precision Medical Center, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China.

B cell expansion with NF-κB and T cell anergy (BENTA) is a rare primary immunodeficiency disorder caused by gain-of-function (GOF) mutations in the gene. Affected patients present with persistent B cell lymphocytosis in early childhood paired with lymphadenopathy and splenomegaly. Until now only six activating mutations from 14 patients have been reported in . Read More

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September 2021

Integrative Neuroinformatics for Precision Prognostication and Personalized Therapeutics in Moderate and Severe Traumatic Brain Injury.

Front Neurol 2021 7;12:729184. Epub 2021 Sep 7.

Neuroscience Research Program, Kleysen Institute for Advanced Medicine, Winnipeg, MB, Canada.

Despite changes in guideline-based management of moderate/severe traumatic brain injury (TBI) over the preceding decades, little impact on mortality and morbidity have been seen. This argues against the "one-treatment fits all" approach to such management strategies. With this, some preliminary advances in the area of personalized medicine in TBI care have displayed promising results. Read More

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September 2021

An amine-functionalized olefin-linked covalent organic framework used for the solid-phase microextraction of legacy and emerging per- and polyfluoroalkyl substances in fish.

J Hazard Mater 2021 Sep 16;423(Pt B):127226. Epub 2021 Sep 16.

Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China. Electronic address:

Due to the environmental persistence and various health problems associated with per- and polyfluoroalkyl substances (PFASs), they have come under increased public scrutiny. However, the efficient extraction of PFASs from complex media remains challenging. Herein, an olefin-linked covalent organic framework (COF-CN) has been prepared via a Knoevenagel condensation reaction, followed by reduction using LiAlH to form an amine-functionalized COF (COF-NH). Read More

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September 2021

Quantitative diffusion MRI of the abdomen and pelvis.

Med Phys 2021 Sep 23. Epub 2021 Sep 23.

Departments of Radiology and Medical Physics, University of Wisconsin-Madison.

Diffusion MRI has enormous potential and utility in the evaluation of various abdominal and pelvic disease processes, including cancer and non-cancer imaging of the liver, prostate, and other organs. Quantitative diffusion MRI is based on acquisitions with multiple diffusion encodings followed by quantitative mapping of diffusion parameters that are sensitive to tissue microstructure. Compared to qualitative diffusion-weighted MRI, quantitative diffusion MRI can improve standardization of tissue characterization as needed for disease detection, staging, and treatment monitoring. Read More

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September 2021

Understanding environmental epigenomics in autism spectrum disorder: an interview with Farah R Zahir.

Authors:
Farah R Zahir

Epigenomics 2021 Sep 23. Epub 2021 Sep 23.

Department of Medical Genetics, University of British Colombia, Vancouver, V6H 3N1, BC, Canada.

In this interview, Dr Farah R Zahir speaks with Storm Johnson, Commissioning Editor for , on her work to date in the field of epigenomics, autism and intellectual disability. Dr Farah R Zahir specializes in the identification of novel genetic and epigenetic causes for neurodevelopmental diseases. Her PhD, awarded in 2011 by the University of British Columbia (UBC), resulted in the characterization of new intellectual disability (ID) syndromes, as well as discovery of several new causative genes for the disorder. Read More

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September 2021

Synthesis and characterization of tracers and development of a fluorescence polarization immunoassay for amantadine with high sensitivity in chicken.

J Food Sci 2021 Sep 21. Epub 2021 Sep 21.

College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food, Beijing Laboratory for Food Quality and Safety, Beijing, People's Republic of China.

Fluorescence polarization immunoassay (FPIA) is a homogeneous and rapid analytical method that is suitable for high-throughput screening of large numbers of samples. However, FPIA typically suffers from lower sensitivity than the well-established enzyme-linked immunosorbent assay (ELISA), limiting its wide application as an analytical tool that can be run with trace levels of an analyte. Herein, a highly sensitive FPIA for detecting amantadine (AMD) in chicken is described. Read More

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September 2021

The human microbiome in transplantation: the past, present, and future.

Curr Opin Organ Transplant 2021 Sep 20. Epub 2021 Sep 20.

The Dumont-UCLA Transplantation Center, Department of Surgery, Division of Liver and Pancreas Transplantation Department of Medicine, Vatche & Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.

Purpose Of Review: Over the past 20 years, DNA sequencing technology has transformed human microbiome research from identity characterizations to metagenomics approaches that reveal how microbials correlate with human health and disease. New studies are showing unprecedented opportunity for deep characterization of the human microbial ecosystem, with benefits to the field of organ transplantation.

Recent Findings: In the present review, we focus on past milestones of human-associated microbiota research, paying homage to microbiota pioneers. Read More

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September 2021

Aptamer-functionalized metal-organic framework-based electrospun nanofibrous composite coating fiber for specific recognition of ultratrace microcystin in water.

J Chromatogr A 2021 Sep 10;1656:462542. Epub 2021 Sep 10.

Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou 350108, People's Republic of China; Engineering Technology Research Center on Reagent and Instrument for Rapid Detection of Product Quality and Food Safety, Fuzhou, Fujian 350108, People's Republic of China.

A novel [email protected]@UiO-66-NH electrospun nanofibrous coating fiber for specific recognition of microcystin-LR (MC-LR) was proposed by using electrospinning, metal-organic frameworks (MOF) seed growth and AuNPs bridging aptamer strategies. Characterization of morphology, structure and stability of the obtained affinity nanofibrous coating fiber were investigated. High loading of MOFs and aptamers on the nanofibrous fiber were achieved and successfully applied for accurate identification of MC-LR by solid-phase microextraction (SPME) coupled with LC-MS. Read More

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September 2021

Novel 4DCT Method to Measure Regional Left Ventricular Endocardial Shortening Before and After Transcatheter Mitral Valve Implantation.

Struct Heart 2021 15;5(4):410-419. Epub 2021 Jul 15.

Department of Bioengineering, University of California San Diego, La Jolla, California, United States.

Background: Regional left ventricular (LV) mechanics in mitral regurgitation (MR) patients, and local changes in function after transcatheter mitral valve implantation (TMVI) have yet to be evaluated. Herein, we introduce a method for creating high resolution maps of endocardial function from 4DCT images, leading to detailed characterization of changes in local LV function. These changes are particularly interesting when evaluating the effect of the Tendyne TMVI device in the region of the epicardial pad. Read More

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Fast and exact quantification of motif occurrences in biological sequences.

BMC Bioinformatics 2021 Sep 18;22(1):445. Epub 2021 Sep 18.

Department of Computer and Information Science and Engineering, University of Florida, Gainesville, FL, USA.

Background: Identification of motifs and quantification of their occurrences are important for the study of genetic diseases, gene evolution, transcription sites, and other biological mechanisms. Exact formulae for estimating count distributions of motifs under Markovian assumptions have high computational complexity and are impractical to be used on large motif sets. Approximated formulae, e. Read More

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September 2021

Methods in sulfide and persulfide research.

Nitric Oxide 2021 Sep 14;116:47-64. Epub 2021 Sep 14.

Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, 980-8575, Japan. Electronic address:

Sulfides and persulfides/polysulfides (R-S-R', n > 2; R-S-H, n > 1) are endogenously produced metabolites that are abundant in mammalian and human cells and tissues. The most typical persulfides that are widely distributed among different organisms include various reactive persulfides-low-molecular-weight thiol compounds such as cysteine hydropersulfide, glutathione hydropersulfide, and glutathione trisulfide as well as protein-bound thiols. These species are generally more redox-active than are other simple thiols and disulfides. Read More

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September 2021

Molecular Deficits Relevant to Concussion Are Prevalent in Top-Ranked Football Players Entering the National Football League Draft.

J Strength Cond Res 2021 Sep 16. Epub 2021 Sep 16.

Onegevity Health, New York, New York; Thorne HealthTech, Summerville, South Carolina; EXOS, Phoenix, Arizona; and Advanced Pattern Analysis & Countermeasures Group, Boulder, Colorado.

Abstract: Kunces, LJ, Keenan, J, Schmidt, CM, and Schmidt, MA. Molecular deficits relevant to concussion are prevalent in top-ranked football players entering the National Football League draft. J Strength Cond Res XX(X): 000-000, 2021-Characterization of blood variants in athletes entering the highly competitive contact environment of professional football can help us understand the risk for brain injury. Read More

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September 2021

Multiparticle Quantum Walks and Fisher Information in One-Dimensional Lattices.

Phys Rev Lett 2021 Sep;127(10):100406

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, APM, Chinese Academy of Sciences, Wuhan 430071, China.

Recent experiments on quantum walks (QWs) demonstrated a full control over the statistics-dependent walks of single particles and two particles in one-dimensional lattices. However, little is known about the general characterization of QWs at the many-body level. Here, we rigorously study QWs, Bloch oscillations, and the quantum Fisher information for three indistinguishable bosons and fermions in one-dimensional lattices using a time-evolving block decimation algorithm and many-body perturbation theory. Read More

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September 2021

Pre-treatment risk assessment of women with endometrial cancer: differences in outcomes of molecular and clinical classifications in the Slovenian patient cohort.

Radiol Oncol 2021 Sep 17. Epub 2021 Sep 17.

Divison of Gynecology and Perinatology, University Medical Centre Maribor, Maribor, Slovenia.

Background: The aim of this study was to evaluate changes in prognostic risk profiles of women with endometrial cancer by comparing the clinical risk assessment with the integrated molecular risk assessment profiling.

Patients And Methods: This prospective study recruited patients with biopsy proven endometrial cancer treated at the University Medical Centre Maribor between January 2020 to February 2021. Patient clinical data was assessed and categorized according to the currently valid European Society of Gynaecological Oncology, European SocieTy for Radiotherapy and Oncology, and European Society of Pathology (ESGO/ESTRO/ESP) guidelines on endometrial cancer. Read More

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September 2021

Novel Characterization of Myeloid-Derived Suppressor Cells in Tumor Microenvironment.

Front Cell Dev Biol 2021 30;9:698532. Epub 2021 Aug 30.

Immunotherapy Laboratory, College of Pharmacology, Southwest Minzu University, Chengdu, China.

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous group of cells generated in various pathologic conditions, which have been known to be key components of the tumor microenvironment (TME) involving in tumor immune tolerance. So MDSCs have been extensively researched recently. As its name suggests, immunosuppression is the widely accepted function of MDSCs. Read More

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Terahertz time-domain ellipsometry with high precision for the evaluation of GaN crystals with carrier densities up to 10 cm.

Sci Rep 2021 Sep 15;11(1):18129. Epub 2021 Sep 15.

Institute of Laser Engineering, Osaka University, Suita, Osaka, 565-0871, Japan.

Gallium nitride (GaN) is one of the most technologically important semiconductors and a fundamental component in many optoelectronic and power devices. Low-resistivity GaN wafers are in demand and actively being developed to improve the performance of vertical GaN power devices necessary for high-voltage and high-frequency applications. For the development of GaN devices, nondestructive characterization of electrical properties particularly for carrier densities in the order of 10 cm or higher is highly favorable. Read More

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September 2021

Multi-platform profiling characterizes molecular subgroups and resistance networks in chronic lymphocytic leukemia.

Nat Commun 2021 Sep 13;12(1):5395. Epub 2021 Sep 13.

Department I for Internal Medicine and Centre for Integrated Oncology, University of Cologne, Cologne, Germany.

Knowledge of the genomic landscape of chronic lymphocytic leukemia (CLL) grows increasingly detailed, providing challenges in contextualizing the accumulated information. To define the underlying networks, we here perform a multi-platform molecular characterization. We identify major subgroups characterized by genomic instability (GI) or activation of epithelial-mesenchymal-transition (EMT)-like programs, which subdivide into non-inflammatory and inflammatory subtypes. Read More

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September 2021

Integrative analysis reveals clinically relevant molecular fingerprints in pancreatic cancer.

Mol Ther Nucleic Acids 2021 Dec 2;26:11-21. Epub 2021 Jul 2.

Precision Research Center for Refractory Diseases, Institute for Clinical Research, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 201620, China.

Pancreatic cancer is a highly aggressive cancer with an exceedingly low rate of response to treatments, which calls for comprehensive molecular characterization of pancreatic cancer cell lines (PCCLs). We screened multi-layer molecular data of 36 PCCLs, including gene mutation, gene expression, microRNA (miRNA) expression, and protein profiles. Our comparative analysis of genomic mutations found that PCCLs recapitulated genomic alterations of the primary tumor and suggested potential therapeutic strategies for clinical interventions. Read More

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December 2021

The value of comprehensive genomic sequencing to maximize the identification of clinically actionable alterations in advanced cancer patients: a case series.

Oncotarget 2021 Aug 31;12(18):1836-1847. Epub 2021 Aug 31.

Translational Genomic Research Institute (Tgen), Phoenix, AZ 85004, USA.

Purpose: We present seven cases of advanced cancer patients who initially underwent tumor testing utilizing smaller, panel-based tests, followed by a variety of therapeutic treatments which ultimately resulted in progression of their disease. These cases demonstrate the value of utilizing WES/RNA seq and characterization following disease progression in these patients and the determination of clinically targetable alterations as well as acquired resistance mutations.

Materials And Methods: All patients are part of an IRB approved observational study. Read More

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Preparation of a Novel Resin Based Covalent Framework Material and Its Application in the Determination of Phenolic Endocrine Disruptors in Beverages by SPE-HPLC.

Polymers (Basel) 2021 Aug 31;13(17). Epub 2021 Aug 31.

School of Chemical Engineering, Liaoning Provincial Key Laboratory of Fine Separation Technique, University of Science and Technology, Liaoning, Anshan 114051, China.

A new type of economical covalent organic framework material(COF), namely resin based covalent organic framework material, was prepared by combining resin and covalent organic framework material by hydrothermal synthesis, which was based on the preparation of traditional COF material(TpBD COF). The properties of the material and covalent organic framework material were compared in the way of characterization, and the possible reaction mechanism was analyzed. The solid phase extraction separation (SPE) ability of this material for four kinds of phenolic endocrine disrupting compounds (bisphenol F, bisphenol A, octylphenol and nonylphenol) in beverage samples was investigated. Read More

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Modeling, Simulation Methods and Characterization of Photon Detection Probability in CMOS-SPAD.

Sensors (Basel) 2021 Aug 31;21(17). Epub 2021 Aug 31.

Department of Electronic Optic and Signal, ISAE-SUPAERO, University of Toulouse, 31055 Toulouse, France.

Single-Photon Avalanche Diodes (SPAD) in Complementary Metal-Oxide Semiconductor (CMOS) technology are potential candidates for future "Light Detection and Ranging" (Lidar) space systems. Among the SPAD performance parameters, the Photon Detection Probability (PDP) is one of the principal parameters. Indeed, this parameter is used to evaluate the SPAD sensitivity, which directly affects the laser power or the telescope diameter of space-borne Lidars. Read More

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Thermal Processing Map and Microstructure Evolution of Inconel 625 Alloy Sheet Based on Plane Strain Compression Deformation.

Materials (Basel) 2021 Sep 3;14(17). Epub 2021 Sep 3.

State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi'an 710072, China.

Plane strain compression tests were used to study the deformation behavior of an Inconel 625 alloy sheet at various temperatures and strain rates. The peak stress was selected to establish the constitutive equation, and the processing maps under different strains were drawn. The results show that the effective stress-strain curve of Inconel 625 has typical dynamic recrystallization (DRX) characteristics. Read More

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September 2021

Forces and Specific Energy of Polyamide Grinding.

Materials (Basel) 2021 Sep 3;14(17). Epub 2021 Sep 3.

Bosch Tecnologie Diesel S.p.A., 70026 Modugno, Italy.

This work investigated the grinding process of reinforced and nonreinforced polyamide materials using an AlO grinding wheel. Samples were ground using a custom-made setup of sensors to evaluate in-line temperature, forces, and power. The surface roughness and images were acquired to assess the quality of the final products. Read More

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September 2021

Monogenic Epilepsies: Disease Mechanisms, Clinical Phenotypes, and Targeted Therapies.

Neurology 2021 Sep 7. Epub 2021 Sep 7.

Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology, London, UK.

A monogenic aetiology can be identified in up to 40% of people with severe epilepsy. To address earlier and more appropriate treatment strategies, clinicians are required to know the implications that specific genetic causes might have on pathophysiology, natural history, comorbidities and treatment choices. In this narrative review, we summarise concepts on the genetic epilepsies based on the underlying pathophysiological mechanisms and present the current knowledge on treatment options based on evidence provided by controlled trials or studies with lower classification of evidence. Read More

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September 2021

Precision sampling fuels precision oncology: an evolutionary perspective.

Trends Cancer 2021 Sep 4. Epub 2021 Sep 4.

Department of Pathology, Cruces University Hospital, 48903 Barakaldo, Bizkaia, Spain; Biocruces-Bizkaia Institute, 48903 Barakaldo, Bizkaia, Spain. Electronic address:

Intratumor heterogeneity (ITH) develops in malignant tumors. Precision sampling that captures this tumor variability is essential for the implementation of precision oncology. We highlight the necessity to update current sampling protocols and implement a strategy to ensure ITH detection and characterization. Read More

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September 2021

High-throughput characterization of photocrosslinker-bearing ion channel variants to map residues critical for function and pharmacology.

PLoS Biol 2021 Sep 7;19(9):e3001321. Epub 2021 Sep 7.

Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.

Incorporation of noncanonical amino acids (ncAAs) can endow proteins with novel functionalities, such as crosslinking or fluorescence. In ion channels, the function of these variants can be studied with great precision using standard electrophysiology, but this approach is typically labor intensive and low throughput. Here, we establish a high-throughput protocol to conduct functional and pharmacological investigations of ncAA-containing human acid-sensing ion channel 1a (hASIC1a) variants in transiently transfected mammalian cells. Read More

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September 2021

Fast myocardial T mapping in mice using k-space weighted image contrast and a Bloch simulation-optimized radial sampling pattern.

MAGMA 2021 Sep 7. Epub 2021 Sep 7.

Department of Internal Medicine I, University Hospital Würzburg, Oberdürrbacher Str. 6, 97080, Würzburg, Germany.

Purpose: T dispersion quantification can potentially be used as a cardiac magnetic resonance index for sensitive detection of myocardial fibrosis without the need of contrast agents. However, dispersion quantification is still a major challenge, because T mapping for different spin lock amplitudes is a very time consuming process. This study aims to develop a fast and accurate T mapping sequence, which paves the way to cardiac T dispersion quantification within the limited measurement time of an in vivo study in small animals. Read More

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September 2021

A randomized, double-blind, placebo-controlled trial of ISC 17536, an oral inhibitor of TRPA1, in patients with painful diabetic peripheral neuropathy: impact of preserved small nerve fiber function.

Pain 2021 Sep 2. Epub 2021 Sep 2.

TOTALL Diabetes Hormone Institute, Indore, India; Kovai Diabetes Speciality Centre and Hospital; Coimbatore, India; Glenmark Pharmaceuticals Limited, Mumbai, India; Ichnos Sciences, Inc., New York, NY; Early Clinical Development Consulting Ltd., Macclesfield, United Kingdom; Department of Neurology, Harvard Medical School, Boston, MA; Division of Neurological Pain Research and Therapy, Universitätsklinikum Schleswig-Holstein, Campus-Kiel, Germany Janssen Pharmaceuticals, Inc., Cambridge, MA.

Abstract: Patients with chronic pain syndromes, such as those with painful peripheral neuropathy due to diabetes mellitus, have limited treatment options and suffer ongoing attrition of their quality of life. Safer and more effective treatment options are needed. One therapeutic approach encompasses phenotypic characterization of the neuropathic pain subtype, combined with the selection of agents that act on relevant mechanisms. Read More

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September 2021