180,034 results match your criteria characterization method

Hydrated electrons mediated in-situ construction of cubic phase CdS/Cd thin layer on a millimeter-scale support for photocatalytic hydrogen evolution.

J Colloid Interface Sci 2021 Sep 8;607(Pt 1):769-781. Epub 2021 Sep 8.

TJU-NIMS International Collaboration Laboratory, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, People's Republic of China; International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.

In this study, non-noble metal Cd decorated cubic phase CdS (CdS/Cd) thin layer on a millimeter-scale chitosan-Mg(OH) xerogel beads (CMB) were elaborately designed and successfully synthesized via facile hydrated electrons (e) assistant strategy. The in-situ formation of metallic Cd was driven by e generated from UV/NaSO process. Owing to metallic Cd, [email protected]/Cd exhibited better visible-light absorption ability and more efficient separation capability for photo-induced carriers, its hydrogen production efficiency was about threefold improved compared to [email protected] Read More

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September 2021

Clinical Characterization of Epilepsy in Children With Angelman Syndrome.

Pediatr Neurol 2021 Aug 31;124:42-50. Epub 2021 Aug 31.

Roche Pharma Research and Early Development, Neuroscience and Rare Diseases, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., Basel, Switzerland. Electronic address:

Background: Epilepsy is highly prevalent in children with Angelman syndrome (AS), and its detailed characterization and relationship to the genotype (deletion vs nondeletion) is important both for medical practice and for clinical trial design.

Methods And Materials: We retrospectively analyzed the main clinical features of epilepsy in 265 children with AS who were enrolled in the AS Natural History Study, a multicenter, observational study conducted at six centers in the United States. Participants were prospectively followed up and classified by genotype. Read More

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Spectroscopy and second hyperpolarizability of odd spin states of acetonitrile: Theoretical study.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 10;265:120389. Epub 2021 Sep 10.

Department of Physics, The Institute of Science, Dr. Homi Bhabha State University, Mumbai, 400032, India.

The present study reports the spectroscopic characterization and second hyperpolarizability of odd spin states of acetonitrile in gas phase and water solvent. The odd spin states of acetonitrile are singlet, triplet and quintet spin state of acetonitrile have been considered for the study. The spectroscopic characterization viz. Read More

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September 2021

PLS-DA and data fusion of visible Reflectance, XRF and FTIR spectroscopy in the classification of mixed historical pigments.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 10;265:120384. Epub 2021 Sep 10.

Laboratório de Instrumentação e Simulação Computacional. LISCOMP/IFRJ-CPAR, 26600-000, Paracambi, Brazil. Electronic address:

In this work samples of historical pigments of green hue were brushed on a canvas and studied by Visible Reflectance, X-Ray Fluorescence and Fourier Transform Infrared Spectroscopy. One hundred samples were investigated, all with green hue, these prepared from pigments themselves green, such as chromium oxide (CrO) or from a mixture of pigments that result in green, for example, chrome yellow (PbCrO) and Prussian blue (Fe[Fe(CN)]). Because every sample investigated through the spectroscopic techniques were of green hue, the characterization of the pigments present in the mixtures through the visual inspection of spectra has become a complex task in some cases, also, due the large number of recorded spectra. Read More

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September 2021

Generation and characterization of a tractable C. elegans model of tauopathy.

Geroscience 2021 Sep 18. Epub 2021 Sep 18.

Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA.

Alzheimer's disease(AD) is an age-associated neurodegenerative disease that results in deterioration of memory and cognitive function. As a currently untreatable disorder, AD has emerged as one of the defining biomedical challenges of our time. Thus, new approaches that can examine the cellular and molecular mechanisms underlying age-related AD pathology are sorely needed. Read More

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September 2021

Ultrasound of the symptomatic shoulder arthroplasty: Spectrum and prevalence of periarticular soft tissue pathology.

J Clin Ultrasound 2021 Sep 18. Epub 2021 Sep 18.

Department of Radiology, Langone Orthopedic Hospital, New York, New York, USA.

Purpose: To describe our experience using ultrasound (US) to evaluate postoperative complications in the presence of in situ shoulder arthroplasty.

Methods: Review of patients who underwent US evaluation following total shoulder arthroplasty (TSA), reverse total shoulder arthroplasty (RTSA) or hemiarthroplasty from 2007 to 2020. All studies were reviewed independently by two musculoskeletal radiologists to assess for joint effusion, periarticular collection, and characterization of associated rotator cuff tears. Read More

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September 2021

T-Cell Specificity Influences Disease Heterogeneity in Multiple Sclerosis.

Neurol Neuroimmunol Neuroinflamm 2021 Nov 17;8(6). Epub 2021 Sep 17.

From the Neuroimmunology and MS Research (NIMS) (C.C., M.P., P.T.O., I.J., M.J.D., R.P., P.M., C.W., I.J., A.L., R.M., M.S.), Department of Neurology, University Hospital and University Zurich, Switzerland; Department of Neuroscience DNS (M.P.), University-Hospital of Padova, Italy; Jung Diagnostics GmbH (R.O.), HIP - Health Innovation Port, Germany; Department of Health Sciences and Technology (C.W.), ETH Zurich, Switzerland; and Clinical Department of Neurology (M.R.), Medical University of Innsbruck, Austria.

Background And Objectives: Encouraged by the enormous progress that the identification of specific autoantigens added to the understanding of neurologic autoimmune diseases, we undertook here an in-depth study of T-cell specificities in the autoimmune disease multiple sclerosis (MS), for which the spectrum of responsible autoantigens is not fully defined yet. The identification of target antigens in MS is crucial for therapeutic strategies aimed to induce antigen-specific tolerance. In addition, knowledge of relevant T-cell targets can improve our understanding of disease heterogeneity, a hallmark of MS that complicates clinical management. Read More

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November 2021

Characterization of variants of uncertain significance in isovaleryl-CoA dehydrogenase identified through newborn screening: An approach for faster analysis.

Mol Genet Metab 2021 Aug 30. Epub 2021 Aug 30.

Division of Genetic and Genomic Medicine, Department of Pediatrics, University of Pittsburgh School of Medicine, and UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA 15224, USA; Department of Human Genetics, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA 15261, USA. Electronic address:

Introduction: Clinical standard of care for newborn screening (NBS) is acylcarnitine metabolites quantitation by tandem mass spectrometry (MS/MS) from dried blood spots. Follow up sequencing often results in identification of one or more variants of uncertain significance (VUS). Isovaleric acidemia (IVA) is an autosomal recessive inborn error of metabolism caused by deficiency of isovaleryl-CoA dehydrogenase (IVDH) in the Leu catabolism pathway. Read More

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Basic pH reversed-phase liquid chromatography (bRPLC) in combination with tip-based strong cation exchange (SCX-Tip), ReST, an efficient approach for large-scale cross-linked peptide analysis.

Anal Chim Acta 2021 Sep 7;1179:338838. Epub 2021 Jul 7.

State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, 200438, China. Electronic address:

Chemical cross-linking in combination with mass spectrometry (XL-MS) has emerged as a useful method for structural elucidation of proteins and protein complexes. Due to the low stoichiometry of cross-linked peptides, a specific enrichment method is always necessary prior to LC-MS/MS analysis, especially for complex samples. Currently, strong cation exchange chromatography (SCX), size exclusion chromatography (SEC), and affinity tag-based enrichment are among the widely used enrichment strategies. Read More

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September 2021

Dopant-assisted atmospheric pressure photoionization Orbitrap mass spectrometry - An approach to molecular characterization of lignin oligomers.

Anal Chim Acta 2021 Sep 9;1179:338836. Epub 2021 Jul 9.

Core Facility Center "Arktika", M.V. Lomonosov Northern (Arctic) Federal University, Northern Dvina Emb. 17, Arkhangelsk, 163002, Russia.

Lignin is the second most abundant biopolymer in nature and is considered an important renewable source of aromatic compounds. One of the most promising analytical methods for molecular characterization of lignin is Orbitrap high-resolution mass spectrometry with atmospheric pressure photoionization (APPI), proved itself in the study of lignins of various origins and their depolymerization products. In this work, the photoionization of lignin using acetone, 1,4-dioxane, and THF as solvents for the biopolymer and APPI dopants providing the generation of protonated and deprotonated molecules of lignin oligomers has been studied. Read More

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September 2021

Low physical activity is associated with impaired endothelial function in patients with type 2 diabetes and controls after 5 years of follow-up.

BMC Endocr Disord 2021 Sep 18;21(1):189. Epub 2021 Sep 18.

Department of Internal Medicine and Endocrinology and Steno Diabetes Center Aarhus, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, DK-8200, Aarhus N, Denmark.

Background: The long-term association between physical activity and endothelial function has not previously been investigated in patients with type 2 diabetes. Therefore, we aimed to evaluate the relationship between physical activity and endothelial function, assessed by peripheral arterial tonometry, in patients with type 2 diabetes and non-diabetic controls after 5 years of follow-up.

Methods: We included 51 patients with newly diagnosed type 2 diabetes and 53 sex- and age matched controls. Read More

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September 2021

Genetic characterization of Carbapenem-Resistant Escherichia coli from China, 2015-2017.

BMC Microbiol 2021 Sep 17;21(1):248. Epub 2021 Sep 17.

Laboratory Medicine Department, First Medical Center of Chinese PLA General Hospital, Beijing, 100853, China.

Background: The molecular characteristics of carbapenem-resistant Escherichia coli (CREco) remain unclear.

Methods: We conducted a multi-center bacterial resistance monitoring project from 2015 to 2017.The minimum inhibitory concentrations ofCREco were determined bybroth microdilution method. Read More

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September 2021

Histologic and Molecular Characterization of Non-Small Cell Lung Carcinoma With Discordant ROS1 Immunohistochemistry and Fluorescence In Situ Hybridization.

Appl Immunohistochem Mol Morphol 2021 Sep 16. Epub 2021 Sep 16.

University of Utah and ARUP Laboratories, Salt Lake City, UT St. Jude Childrens' Research Hospital, Memphis, TN.

Introduction: ROS1 immunohistochemical (IHC) positivity requires follow-up with confirmatory testing such as fluorescence in situ hybridization (FISH). Identifying predictive characteristics of false positive ROS1 IHC cases could aid in optimizing testing algorithms, decrease testing costs and preserve tissue.

Materials And Methods: Retrospective results were retrieved for 2054 patients with non-small cell lung carcinoma submitted to our laboratory for molecular testing. Read More

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September 2021

Temperature-dependent dielectric properties of human uterine fibroids over microwave frequencies.

Biomed Phys Eng Express 2021 Sep 17. Epub 2021 Sep 17.

Electrical and Computer Engineering, Kansas State University College of Engineering, 3108 Engineering Hall Manhattan, Kansas 66506-5200, Manhattan, Kansas, 66506-5201, UNITED STATES.

Microwave ablation is under investigation as a minimally-invasive treatment for uterine fibroids. Computational models play a vital role in the development, evaluation and characterization of candidate ablation devices. The temperature-dependent dielectric properties of fibroid tissue are essential for accurate computational modeling. Read More

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September 2021

Cross-oceanic distribution and origin of microplastics in the subsurface water of the South China Sea and Eastern Indian Ocean.

Sci Total Environ 2021 Sep 10;805:150243. Epub 2021 Sep 10.

State Key Laboratory of Estuarine and Costal Research, East China Normal University, Shanghai 200062, China; Plastic Marine Debris Research Center, East China Normal University, Shanghai 200241, China; Region Training and Research Center on Plastic Marine Debris and Microplastics, IOC-UNESCO, 200241, China. Electronic address:

Marine microplastic (MP) pollution is a global environmental problem that has received attention from scientific researchers and the public for the past several decades. However, without a suitably large-volume sampling method, the presence of MPs in subsurface water (< 5 m) is poorly understood. Here, MP content in subsurface water was determined using a pump-underway ship intake system along the cross-oceanic transect from the Pearl River Estuary to the Indian Ocean. Read More

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September 2021

Defect engineering in polymeric carbon nitride photocatalyst: Synthesis, properties and characterizations.

Adv Colloid Interface Sci 2021 Sep 9;296:102523. Epub 2021 Sep 9.

Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

Polymer carbon nitride (CN) has unique structure and electronic properties, making it attractive in photocatalysis fields. However, the photocatalytic efficiency of the pristine CN photocatalyst is still unsatisfactory. In this regard, the introduction of vacancy defects can effectively tune photoelectric properties of CN photocatalyst through tailoring the electronic structure and bandgap engineering. Read More

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September 2021

Comparative study of peripheral blood cells in two marine fishes (Synechogobius hasta and Sebastes schlegelii): Morphological and cytochemical characterization.

Tissue Cell 2021 Aug 30;73:101633. Epub 2021 Aug 30.

Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, China; Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, China. Electronic address:

The morphology, cell size and relative number of peripheral blood cells in two teleosts, Synechogobius hasta and Sebastes schlegelii, were compared using different staining methods. The results showed significant differences in cell size. The percentage of monocytes in S. Read More

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Characterization of inflammatory changes in the breast cancer associated adipose tissue and comparison to the unaffected contralateral breast.

Surg Oncol 2021 Sep 9;39:101659. Epub 2021 Sep 9.

The Ohio State University Comprehensive Cancer Center, The Ohio State University, 410 W 12th Avenue, Columbus, OH, 43210, USA; Department of Surgery, The Ohio State University, 410 W 10th Ave, N911 Doan Hall, Columbus, OH, 43210, USA. Electronic address:

Background: Adipose tissue has emerged as an important window into cancer pathophysiology, revealing potential targets for novel therapeutic interventions. The goal of this study was to compare the breast adipose tissue (BrAT) immune milieu surrounding breast carcinoma and contralateral unaffected breast tissue obtained from the same patient.

Materials And Methods: Patients undergoing bilateral mastectomy for unilateral breast cancer were enrolled for bilateral BrAT collection at the time of operation. Read More

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September 2021

Impact of electrode size and spacing on electrograms: Optimal electrode configuration for near-field electrogram characterization.

Heart Rhythm 2021 Sep 14. Epub 2021 Sep 14.

CHU Bordeaux, IHU LIRYC, Univ. Bordeaux, Bordeaux, France.

Background: Detailed effects of electrode size on EGMs have not been systematically examined.

Objectives: We aimed to elucidate the effect of electrode size on EGMs and investigate an optimal configuration of electrode-size and interelectrode-spacing for gap detection and far-field reduction.

Methods: This study included 8 sheep where probes with different electrode-size and interelectrode-spacing were epicardially placed on healthy, fatty, and lesion tissues for measurements. Read More

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September 2021

Reverse-dialysis can be misleading for drug release studies in emulsions as demonstrated by NMR dilution experiments.

Int J Pharm 2021 Sep 14:121093. Epub 2021 Sep 14.

Department of Chemistry, University of Miami, Coral Gables, FL 33146. Electronic address:

Emulsions are an important class of carriers for the delivery of hydrophobic drugs. While knowledge of drug release kinetics is critical to optimizing drug carrying emulsions, there remain many open questions about the validity of standard characterization methods such as the commonly used reverse-dialysis. In this paper, the kinetic parameters of isoflurane release in perfluorotributylamine emulsions determined from both reverse-dialysis and nuclear magnetic resonance (NMR) dilution experiments are compared. Read More

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September 2021

Methods in sulfide and persulfide research.

Nitric Oxide 2021 Sep 14;116:47-64. Epub 2021 Sep 14.

Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, 980-8575, Japan. Electronic address:

Sulfides and persulfides/polysulfides (R-S-R', n > 2; R-S-H, n > 1) are endogenously produced metabolites that are abundant in mammalian and human cells and tissues. The most typical persulfides that are widely distributed among different organisms include various reactive persulfides-low-molecular-weight thiol compounds such as cysteine hydropersulfide, glutathione hydropersulfide, and glutathione trisulfide as well as protein-bound thiols. These species are generally more redox-active than are other simple thiols and disulfides. Read More

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September 2021

Follicular Helper-like T Cells in the Lung Highlight a Novel Role of B Cells in Sarcoidosis.

Am J Respir Crit Care Med 2021 Sep 17. Epub 2021 Sep 17.

University Hospital Schleswig Holstein, 54186, Institute of Immunology, Kiel, Germany;

Rationale: Pulmonary sarcoidosis is generally presumed to be a T helper-1- and macrophage-driven disease. However, mouse models have recently revealed that chronically inflamed lung tissue can also comprise T follicular helper- (Tfh-) like cells and represents a site of active T cell / B cell cooperation.

Objectives: To assess the role of pulmonary Tfh- and germinal center-like lymphocytes in sarcoidosis. Read More

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September 2021

Methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage among patients with diabetes at the Korle Bu Teaching Hospital.

PLoS One 2021 17;16(9):e0257004. Epub 2021 Sep 17.

Department of Medical Microbiology, University of Ghana Medical School, Korle Bu, Accra, Ghana.

Aim: To investigate the epidemiology of S. aureus and MRSA nasal carriage among people with diabetes at the Korle Bu Teaching Hospital in Accra, including the prevalence, predictors of carriage, and antibiotic resistance.

Methodology: This study was cross-sectional, involving 300 diabetes patients and 106 non-diabetic individuals. Read More

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September 2021

Characterization of fecal metabolome changes in patients with obstructive sleep apnea disease.

J Clin Sleep Med 2021 Sep 17. Epub 2021 Sep 17.

Heart Center & Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Study Objectives: Obstructive sleep apnea (OSA) is a clinical syndrome characterized by recurrent episodes of apnea or hypopnea of the upper airway, leading to increased negative intrathoracic pressure, sleep fragmentation, intermittent hypoxia during sleep, and increased risk for morbidity and mortality of affected patients. The gut microbiome plays a key role in OSA pathogenesis, and fecal metabolic profiling reflects the gut microbial functional readout and mediates host-microbiome interactions.

Methods: Herein, we conducted a cohort study to explore fecal metabolic signatures distinguishing OSA (44 patients) from healthy controls (22 healthy controls) by untargeted gas chromatography- time of flight mass spectroscopy. Read More

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September 2021

Exploring the Complexity of Aphasia With Network Analysis.

J Speech Lang Hear Res 2021 Sep 17:1-14. Epub 2021 Sep 17.

Department of Communicative Disorders and Sciences, University at Buffalo, NY.

Purpose Aphasia is a complex, neurogenic language disorder, with different aphasia syndromes hallmarked by impairment in fluency, auditory comprehension, naming, and/or repetition. Broad, standardized assessments of language domains and specific language and cognitive assessments provide a holistic impairment profile of a person with aphasia. While many recognize the correlations between assessments, there remains a need to continue understanding the complexity of relationships between assessments for the purpose of better characterization of language impairment profiles of persons with aphasia. Read More

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September 2021

Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering.

Hum Cell 2021 Sep 17. Epub 2021 Sep 17.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Since polymeric nanofibrous scaffolds have been widely used in tissue regeneration, the risk of bacterial infections should not be neglected. In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration. The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, and water contact angle tests. Read More

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September 2021

Characteristic phenotype of Chinese patients with adult-onset diabetes who are autoantibody positive by 3-Screen ICA™ ELISA.

Acta Diabetol 2021 Sep 17. Epub 2021 Sep 17.

NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China.

Aims: To assess the prevalence of diabetes-associated autoantibodies in Chinese patients recently diagnosed with adult-onset diabetes and to evaluate the potential role of the autoantibody markers for characterization of disease phenotype in the patient population.

Methods: The study included 1273 recent-onset adult patients with phenotypic type 2 diabetes mellitus (T2DM). Serum samples were tested using the 3-Screen ICA™ ELISA (3-Screen) designed for combined measurement of GADAb and/or IA-2Ab and/or ZnT8Ab. Read More

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September 2021

Development and characterization of a unique anti-IgE mouse monoclonal antibody cross-reactive between human and canine IgE.

Immun Inflamm Dis 2021 Sep 17. Epub 2021 Sep 17.

Animal Allergy Clinical Laboratories Inc., Sagamihara, Kanagawa, Japan.

Background: The efficacy assessment of human anti-IgE monoclonal antibodies (mAbs) in animal models before clinical trials is hampered due to the lack of cross-reactivity of anti-IgE mAbs between species.

Objective: We developed CRE-DR (an anti-dog IgE monoclonal antibody), an anti-IgE mouse mAb that recognizes canine and human IgE, and then examined its IgE specificity and cross-reactivity between three animal and human species.

Methods: After mouse immunization with a synthetic peptide derived from canine IgE ( NTNDWIEGETYYC ), we generated a hybridoma producing CRE-DR. Read More

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September 2021

In-plane ferroelectricity in few-layered GeS and its van der Waals ferroelectric diodes.

Nanoscale 2021 Sep 17. Epub 2021 Sep 17.

School of Physics, Henan Key Laboratory of Photovoltaic Materials, Henan Normal University, Henan 453007, China.

Two-dimensional ferroelectric semiconductors (2DFeSs) have been attracting extensive research attention on account of their unique properties and versatile applications in random-access memory, digital signal processors, and neuromorphic computing. Germanium sulfide (GeS) is predicted as a typical 2DFeS with a large spontaneous polarization of 484 pC m. Furthermore, the moderate band gap equivalent to 1. Read More

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September 2021

Localized surface plasmon resonance for enhanced electrocatalysis.

Chem Soc Rev 2021 Sep 17. Epub 2021 Sep 17.

Department of Chemistry, Key Lab of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing 100084, China.

Electrocatalysis plays a vital role in energy conversion and storage in modern society. Localized surface plasmon resonance (LSPR) is a highly attractive approach to enhance the electrocatalytic activity and selectivity with solar energy. LSPR excitation can induce the transfer of hot electrons and holes, electromagnetic field enhancement, lattice heating, resonant energy transfer and scattering, in turn boosting a variety of electrocatalytic reactions. Read More

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September 2021