43,340 results match your criteria channels expressed

Inhibition of TRPV1 and TRPA1 by mosquito and mouse saliva.

Pain 2021 May 10. Epub 2021 May 10.

Division of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural Sciences (NIPS), Okazaki, 444-8787 Japan Thermal Biology Group, Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, 444-8787 Japan Department of Physiological Sciences, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, 444-8787 Japan Faculty of Engineering Science, Kansai University, Osaka 564-8680, Japan Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, University of Toyama, Toyama, 930-0194 Japan.

Abstract: Arthropods are the largest group of living organisms and among them, mosquitoes spread parasites and viruses causing deathly diseases. They can spread easily these pathogens because of their painless skin piercing. Although the lack of pain is mainly due to the thinness of their fascicle, it is possible that mosquito saliva, which is discharged during their piercing, might contribute. Read More

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SET knockdown attenuated phenotype modulation and calcium channel associated markers of airway smooth muscle cells in asthmatic mice.

Ann Transl Med 2021 Apr;9(8):657

Key Laboratory of Shenzhen Respiratory Disease, Shenzhen Institute of Respiratory Disease, Shenzhen People's Hospital (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University), Shenzhen, China.

Background: Dysfunctional phenotype modulation and calcium channels in airway smooth muscle cells (ASMCs) are important characteristics of airway remodeling in chronic asthma. However, the mechanisms underlying these pathological processes remain unclear. SET (I2PP2A, inhibitor-2 of protein phosphatase 2A) has many significant functions and is involved in various physiological and pathological processes. Read More

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Homocysteine alters vasoreactivity of human internal mammary artery by affecting the K channel family.

Ann Transl Med 2021 Apr;9(8):625

Center for Basic Medical Research & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Background: Hyperhomocysteinemia is an independent risk factor for atherosclerotic heart disease. We previously demonstrated that disruption of calcium-activated potassium (K) channel activity is involved in homocysteine-induced dilatory dysfunction of porcine coronary arteries. Recently we reported that the K channel family, including large-, intermediate-, and small-conductance K (BK, IK, and SK) subtypes, are abundantly expressed in human internal mammary artery (IMA). Read More

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Rab11-dependent recycling of calcium channels is mediated by auxiliary subunit αδ-1 but not αδ-3.

Sci Rep 2021 May 13;11(1):10256. Epub 2021 May 13.

Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK.

N-type voltage-gated calcium channels (Ca2.2) are predominantly expressed at presynaptic terminals, and their function is regulated by auxiliary αδ and β subunits. All four mammalian αδ subunits enhance calcium currents through Ca1 and Ca2 channels, and this increase is attributed, in part, to increased Ca expression at the plasma membrane. Read More

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Clinical and genetic analysis of KATP variants with heart failure risk in patients with decreased serum ApoA-I levels.

J Clin Endocrinol Metab 2021 May 13. Epub 2021 May 13.

Department of Cardiology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou 510180, China.

Context: Lower serum concentration of apolipoprotein A-I (ApoA-I) is causally associated with heart failure (HF) risk. ATP-sensitive potassium channels (KATP), as a gating channel coupling vascular reactivity and metabolism with ischemic protection, become a new potential target of management for HF. The KATP gene sequence is highly polymorphic and high degree of genetic heterogeneity. Read More

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Autoimmune regulation of chronic pain.

Pain Rep 2021 9;6(1):e905. Epub 2021 Mar 9.

Laboratories of Neuroimmunology, Department of Symptom Research, University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Chronic pain is an unpleasant and debilitating condition that is often poorly managed by existing therapeutics. Reciprocal interactions between the nervous system and the immune system have been recognized as playing an essential role in the initiation and maintenance of pain. In this review, we discuss how neuroimmune signaling can contribute to peripheral and central sensitization and promote chronic pain through various autoimmune mechanisms. Read More

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Role of Transient Receptor Potential Vanilloid 4 in Vascular Function.

Front Mol Biosci 2021 26;8:677661. Epub 2021 Apr 26.

Wuxi School of Medicine, Jiangnan University, Wuxi, China.

Transient receptor potential vanilloid 4 (TRPV4) channels are widely expressed in systemic tissues and can be activated by many stimuli. TRPV4, a Ca-permeable cation channel, plays an important role in the vasculature and is implicated in the regulation of cardiovascular homeostasis processes such as blood pressure, vascular remodeling, and pulmonary hypertension and edema. Within the vasculature, TRPV4 channels are expressed in smooth muscle cells, endothelial cells, and perivascular nerves. Read More

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PIEZO ion channel is required for root mechanotransduction in .

Proc Natl Acad Sci U S A 2021 May;118(20)

Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037;

Plant roots adapt to the mechanical constraints of the soil to grow and absorb water and nutrients. As in animal species, mechanosensitive ion channels in plants are proposed to transduce external mechanical forces into biological signals. However, the identity of these plant root ion channels remains unknown. Read More

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The anchor domain is critical for Piezo1 channel mechanosensitivity.

Channels (Austin) 2021 Dec;15(1):438-446

Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, Sydney, Australia.

The mechanosensitive channel Piezo1 is a crucial membrane mechanosensor ubiquitously expressed in mammalian cell types. Critical to its function in mechanosensory transduction is its ability to change conformation in response to applied mechanical force. Here, we interrogate the role of the anchor domain in the mechanically induced gating of human Piezo1 channels. Read More

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December 2021

Characterization and expression analysis of tandemly duplicated nicotinic acetylcholine receptors in pearl oysters after stimulation of pathogen-related molecular patterns.

Comp Biochem Physiol B Biochem Mol Biol 2021 May 8:110615. Epub 2021 May 8.

Fishery College, Guangdong Ocean University, Zhanjiang 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China. Electronic address:

Nicotinic acetylcholine receptors (nAChRs) are a class of ligand-gated ion channels that participate in signal transduction and are reported to play an important role in the immunomodulation of vertebrates and invertebrates. Previous studies have shown that the nAChRs in mollusks have undergone large-scale expansion after tandem repeats and retrotransposition, with the most expansion observed in bivalves. This study characterized the sequence of a tandem repeat nAChR unique to several bivalve mollusks and investigated its functions in Pinctada fucata martensii. Read More

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Transcriptome of Cenostigma pyramidale roots, a woody legume, under different salt stress times.

Physiol Plant 2021 May 10. Epub 2021 May 10.

Departamento de Botânica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.

Salinity stress has a significant impact on the gain of plant biomass. Our study provides the first root transcriptome of Cenostigma pyramidale, a tolerant woody legume from a tropical dry forest, under three different salt stress times (30 min, 2 h, and 11 days). The transcriptome was assembled using the RNA sequencing (RNA-Seq) de novo pipeline from GenPipes. Read More

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Microfabricated Device to Record Axonal Conduction under Pharmacological Treatment for Functional Evaluation of Axon Ion Channel.

IEEE Trans Biomed Eng 2021 May 10;PP. Epub 2021 May 10.

Objective: Neuronal networks are fundamental structures for information processing in the central nervous system. This processing function is severely impaired by abnormal axonal conduction from changes in functional ion channel expression. The evaluation of axonal conduction properties can be effective in the early diagnosis of information-processing abnormalities. Read More

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Inward calcium current through the polycystin-2-dependent channels of renal primary cilia.

Am J Physiol Renal Physiol 2021 May 10. Epub 2021 May 10.

Pharmacology & Systems Physiology, University of Cincinnati, United Statesgrid.24827.3b.

In 15% of cases, autosomal dominant polycystic kidney disease (ADPKD) arises from defects in polycystin-2 (PC2). PC2 is a member of the TRPP subfamily of cation-conducting channels and is expressed in the endoplasmic reticulum and primary cilium of renal epithelial cells. PC2 opposes a pro-cystogenic influence of the cilium, and it has been proposed that this beneficial effect is mediated in part by a flow of Ca through PC2 channels into the primary cilium. Read More

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Inhibition of Small-Conductance, Ca-Activated K Current by Ondansetron.

Front Pharmacol 2021 22;12:651267. Epub 2021 Apr 22.

Division of Cardiology, Department of Medicine, The Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, IN, United States.

Small-conductance Ca-activated K channels (SK channels) have been proposed as antiarrhythmic targets for the treatment of atrial fibrillation. We previously demonstrated that the 5-HT receptor antagonist ondansetron inhibits heterologously expressed, human SK2 (hSK2) currents as well as native cardiac SK currents in a physiological extra-/intracellular [K] gradient at therapeutic (i.e. Read More

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Developmental Profile of Psychiatric Risk Associated With Voltage-Gated Cation Channel Activity.

Biol Psychiatry 2021 Mar 13. Epub 2021 Mar 13.

Neuroscience and Mental Health Research Institute, Cardiff University, Cardiff, United Kingdom; MRC Centre for Neuropsychiatric Genetics and Genomics, Division of Psychological Medicine and Clinical Neurosciences, Cardiff University, Cardiff, United Kingdom.

Background: Recent breakthroughs in psychiatric genetics have implicated biological pathways onto which genetic risk for psychiatric disorders converges. However, these studies do not reveal the developmental time point(s) at which these pathways are relevant.

Methods: We aimed to determine the relationship between psychiatric risk and developmental gene expression relating to discrete biological pathways. Read More

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Dual role of miR-1 in the development and function of sinoatrial cells.

J Mol Cell Cardiol 2021 May 6;157:104-112. Epub 2021 May 6.

The Cell Physiology MiLAb; Department of Biosciences, Università degli Studi di Milano, Milan, Italy. Electronic address:

miR-1, the most abundant miRNA in the heart, modulates expression of several transcription factors and ion channels. Conditions affecting the heart rate, such as endurance training and cardiac diseases, show a concomitant miR-1 up- or down-regulation. Here, we investigated the role of miR-1 overexpression in the development and function of sinoatrial (SAN) cells using murine embryonic stem cells (mESC). Read More

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Exploring the oxygenase function of Form II Rubisco for production of glycolate from CO.

AMB Express 2021 May 8;11(1):65. Epub 2021 May 8.

CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

The oxygenase activity of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) converts ribulose-1,5-bisphosphate (RuBP) into 2-phosphoglycolate, which in turn channels into photorespiration, resulting in carbon and energy loss in higher plants. We observed that glycolate can be accumulated extracellularly when two genes encoding the glycolate dehydrogenase of cyanobacteria Synechocystis sp. PCC 6803 were inactivated. Read More

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LncRNA SAMD12-AS1 promotes the progression of gastric cancer via DNMT1/p53 axis.

Arch Med Res 2021 May 4. Epub 2021 May 4.

Department of General Surgery, The No.967 Hospital of PLA Joint Logistics Support Force, Postgraduate Culture Base of Jinzhou Medical University, Dalian, China; Department of General Surgery, The No.967 Hospital of PLA Joint Logistics Support Force, Postgraduate Culture Base of Dalian Medical University, Dalian, China. Electronic address:

Background: Long noncoding RNAs (lncRNAs) are essential modulators of cancers initiation and progression via regulating gene expression and biological behaviors. LncRNA SAMD12-AS1 has been validated to promote the progression of several cancers, while its role in gastric cancer (GC) remains unclear. This study aims to explore the role of LncRNA SAMD12-AS1 in GC. Read More

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To hum or not to hum: Neural transcriptome signature of male courtship vocalization in a teleost fish.

Genes Brain Behav 2021 May 7:e12740. Epub 2021 May 7.

Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States.

For many animal species, vocal communication is a critical social behavior and often a necessary component of reproductive success. Additionally, vocalizations are often demanding motor acts. Wanting to know whether a specific molecular toolkit might be required for vocalization, we used RNA-sequencing to investigate neural gene expression underlying the performance of an extreme vocal behavior, the courtship hum of the plainfin midshipman fish (Porichthys notatus). Read More

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Large-Conductance Calcium-Activated Potassium Channels and Voltage-Dependent Sodium Channels in Human Cementoblasts.

Front Physiol 2021 20;12:634846. Epub 2021 Apr 20.

Department of Physiology, Tokyo Dental College, Tokyo, Japan.

Cementum, which is excreted by cementoblasts, provides an attachment site for collagen fibers that connect to the alveolar bone and fix the teeth into the alveolar sockets. Transmembrane ionic signaling, associated with ionic transporters, regulate various physiological processes in a wide variety of cells. However, the properties of the signals generated by plasma membrane ionic channels in cementoblasts have not yet been described in detail. Read More

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Protocol for a prospective, observational, deep phenotyping study on adipose epigenetic and lipidomic determinants of metabolic homoeostasis in South Asian Indians: the Indian Diabetes and Metabolic Health (InDiMeT) study.

BMJ Open 2021 May 6;11(5):e043644. Epub 2021 May 6.

Division of Nutrition, St John's National Academy of Health Sciences, Bangalore, Karnataka, India

Introduction: We describe the rationale and broad study design of the Indian Diabetes and Metabolic Health (InDiMeT) study, a new prospective, observational study incorporating extensive epigenetic (DNA methylation) and lipidomic signatures to examine their association with the dysregulation of adipose de novo lipogenesis (DNL) in South Asian Indians. The InDiMeT study aims to use a case-control design to identify genetic and modifiable-environmental-lifestyle associated determinants of (1) epigenomic (DNA methylome) dysregulation of adipose DNL in type 2 diabetes mellitus (T2DM) adipose tissue, (2) identify correlates of epigenomic (DNA methylome) dysregulation of adipose DNL in peripheral blood mononuclear cells (PBMCs) from T2DM subjects and (3) elucidate plasma lipidomic correlates of adipose DNL in T2DM that can be used as biomarkers of adipose tissue dysfunction.

Methods And Analysis: The InDiMeT study will involve recruitment of 176 normoglycaemic and T2DM individuals who will be undergoing laparoscopic surgery for clinical conditions. Read More

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Characterization of the HCN Interaction Partner TRIP8b/PEX5R in the Intracardiac Nervous System of TRIP8b-Deficient and Wild-Type Mice.

Int J Mol Sci 2021 Apr 30;22(9). Epub 2021 Apr 30.

Division of Cardiology, EVK Düsseldorf, cNEP, Cardiac Neuro- and Electrophysiology Research Consortium, Kirchfeldstrasse 40, 40217 Düsseldorf, Germany.

The tetratricopeptide repeat-containing Rab8b-interacting protein (TRIP8b/PEX5R) is an interaction partner and auxiliary subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, which are key for rhythm generation in the brain and in the heart. Since TRIP8b is expressed in central neurons but not in cardiomyocytes, the TRIP8b-HCN interaction has been studied intensely in the brain, but is deemed irrelevant in the cardiac conduction system. Still, to date, TRIP8b has not been studied in the intrinsic cardiac nervous system (ICNS), a neuronal network located within epicardial fat pads. Read More

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Effects of electromagnetic fields on neuronal ion channels: a systematic review.

Ann N Y Acad Sci 2021 May 4. Epub 2021 May 4.

Leverhulme Quantum Biology Doctoral Training Centre, University of Surrey, Guildford, Surrey, UK.

Many aspects of chemistry and biology are mediated by electromagnetic field (EMF) interactions. The central nervous system (CNS) is particularly sensitive to EMF stimuli. Studies have explored the direct effect of different EMFs on the electrical properties of neurons in the last two decades, particularly focusing on the role of voltage-gated ion channels (VGCs). Read More

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The N terminus of Orai1 couples to the AKAP79 signaling complex to drive NFAT1 activation by local Ca entry.

Proc Natl Acad Sci U S A 2021 May;118(19)

Department of Physiology, Anatomy and Genetics, Oxford University, Oxford OX1 3PT, United Kingdom;

To avoid conflicting and deleterious outcomes, eukaryotic cells often confine second messengers to spatially restricted subcompartments. The smallest signaling unit is the Ca nanodomain, which forms when Ca channels open. Ca nanodomains arising from store-operated Orai1 Ca channels stimulate the protein phosphatase calcineurin to activate the transcription factor nuclear factor of activated T cells (NFAT). Read More

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Kcns3 deficiency disrupts Parvalbumin neuron physiology in mouse prefrontal cortex: Implications for the pathophysiology of schizophrenia.

Neurobiol Dis 2021 Apr 30:105382. Epub 2021 Apr 30.

Translational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA. Electronic address:

The unique fast spiking (FS) phenotype of cortical parvalbumin-positive (PV) neurons depends on the expression of multiple subtypes of voltage-gated potassium channels (Kv). PV neurons selectively express Kcns3, the gene encoding Kv9.3 subunits, suggesting that Kcns3 expression is critical for the FS phenotype. Read More

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C-bouton components on rat extensor digitorum longus motoneurons are resistant to chronic functional overload.

J Anat 2021 May 3. Epub 2021 May 3.

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology, University College London, London, UK.

Mammalian motor systems adapt to the demands of their environment. For example, muscle fibre types change in response to increased load or endurance demands. However, for adaptations to be effective, motoneurons must adapt such that their properties match those of the innervated muscle fibres. Read More

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5-Hydroxytryptamine, Glutamate, and ATP: Much More Than Neurotransmitters.

Front Cell Dev Biol 2021 15;9:667815. Epub 2021 Apr 15.

Department Biochemistry and Molecular Biomedicine, School of Biology, University of Barcelona, Barcelona, Spain.

5-hydroxytryptamine (5-HT) is derived from the essential amino acid L-tryptophan. Although the compound has been studied extensively for its neuronal handling and synaptic actions, serotonin 5-HT receptors can be found extra-synaptically and not only in neurons but in many types of mammalian cells, inside and outside the central nervous system (CNS). In sharp contrast, glutamate (Glu) and ATP are better known as metabolism-related molecules, but they also are neurotransmitters, and their receptors are expressed on almost any type of cell inside and outside the nervous system. Read More

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Pannexin 1 Influences Lineage Specification of Human iPSCs.

Front Cell Dev Biol 2021 16;9:659397. Epub 2021 Apr 16.

Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, NL, Canada.

Every single cell in the body communicates with nearby cells to locally organize activities with their neighbors and dysfunctional cell-cell communication can be detrimental during cell lineage commitment, tissue patterning and organ development. Pannexin channels (PANX1, PANX2, and PANX3) facilitate purinergic paracrine signaling through the passage of messenger molecules out of cells. PANX1 is widely expressed throughout the body and has recently been identified in human oocytes as well as 2 and 4-cell stage human embryos. Read More

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Comprehensive RNA-Seq Analysis of Potential Therapeutic Targets of Gan-Dou-Fu-Mu Decoction for Treatment of Wilson Disease Using a Toxic Milk Mouse Model.

Front Pharmacol 2021 15;12:622268. Epub 2021 Apr 15.

Department of Pharmacy, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China.

Gan-Dou-Fu-Mu decoction (GDFMD) improves liver fibrosis in experimental and clinical studies including those on toxic mouse model of Wilson disease (Model). However, the mechanisms underlying the effect of GDFMD have not been characterized. Herein, we deciphered the potential therapeutic targets of GDFMD using transcriptome analysis. Read More

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PIP regulation of TRPC5 channel activation and desensitization.

J Biol Chem 2021 Apr 29:100726. Epub 2021 Apr 29.

Department of Pharmaceutical Sciences, School of Pharmacy, Bouvé College of Health Sciences; Center for Drug Discovery; Department of Chemistry and Chemical Biology, College of Science, Northeastern University, Boston, MA. Electronic address:

Transient receptor potential canonical type 5 (TRPC5) ion channels are expressed in the brain and kidney, and have been identified as promising therapeutic targets whose selective inhibition can protect against diseases driven by a leaky kidney filter, such as Focal Segmental Glomerular Sclerosis (FSGS). TRPC5 channels are activated by elevated levels of extracellular Caor lanthanide ions, but also by G protein (G) stimulation. Phosphatidylinositol bisphosphate (PIP) hydrolysis by phospholipase C (PLC) enzymes leads to protein kinase C (PKC)-mediated phosphorylation of TRPC5 channels and their subsequent desensitization. Read More

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