45,418 results match your criteria channel current

Paper-based mediatorless enzymatic microfluidic biofuel cells.

Biosens Bioelectron 2021 Jun 9;190:113391. Epub 2021 Jun 9.

Dept. of Mechanical Engineering, BK21 FOUR ERICA-ACE Center, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, South Korea. Electronic address:

In this study, eco-friendly and disposable paper-based membraneless microfluidic enzymatic fuel cells (EFCs) were developed without any mediators to reduce the toxicity and cost of EFCs. Glucose oxidase and laccase were immobilized on multi-walled carbon nanotube electrodes to catalyze the redox reaction of glucose and oxygen. Micromachining techniques well-suited for mass production were used to precisely fabricate micro-scale Y-shaped and cross-shaped EFCs. Read More

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Chloride channel accessory 1 integrates chloride channel activity and mTORC1 in aging-related kidney injury.

Aging Cell 2021 Jun 12:e13407. Epub 2021 Jun 12.

Department of Medicine, Center for Renal Precision Medicine, University of Texas Health, San Antonio, TX, USA.

The mechanism of kidney injury in aging are not well understood. In order to identify hitherto unknown pathways of aging-related kidney injury, we performed RNA-Seq on kidney extracts of young and aged mice. Expression of chloride (Cl) channel accessory 1 (CLCA1) mRNA and protein was increased in the kidneys of aged mice. Read More

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Prenatal exercise reprograms the development of hypertension progress and improves vascular health in SHR offspring.

Vascul Pharmacol 2021 Jun 8:106885. Epub 2021 Jun 8.

Department of Exercise Physiology, Beijing Sport University, Beijing 100084, China; Key Laboratory of Physical Fitness and Exercise, Ministry of Education, Beijing Sport University, Beijing, China. Electronic address:

Background: Upregulation of L-type voltage-gated Ca (Ca1.2) channel in the arterial myocytes is a hallmark feature of hypertension. However, whether maternal exercise during pregnancy has a sustained beneficial effect on the offspring of spontaneously hypertensive rats (SHRs) through epigenetic regulation of Ca1. Read More

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Altered spontaneous cortical activity in mild glaucoma: A quantitative EEG study.

Neurosci Lett 2021 Jun 8:136036. Epub 2021 Jun 8.

Stress and cognitive electroimaging laboratory, Department of Physiology, All India Institute of Medical Sciences, New Delhi, India. Electronic address:

Functional neuroimaging studies have reported alterations in cortical activity indicating glaucoma as a progressive neurodegenerative disease. Hence the current study aimed to assess the cortical activity using high-density EEG in patients with mild glaucoma during resting state. Treatment-naive 37 patients with primary open angle glaucoma (POAG), 34 patients with primary angle closure glaucoma (PACG), and 32 healthy controls were included in the study. Read More

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Long QT syndrome - Bench to bedside.

Heart Rhythm O2 2021 Feb 22;2(1):89-106. Epub 2021 Jan 22.

Department of Physiology and Cell Biology, The Ohio State University, Columbus, Ohio.

Long QT syndrome (LQTS) is a cardiovascular disorder characterized by an abnormality in cardiac repolarization leading to a prolonged QT interval and T-wave irregularities on the surface electrocardiogram. It is commonly associated with syncope, seizures, susceptibility to torsades de pointes, and risk for sudden death. LQTS is a rare genetic disorder and a major preventable cause of sudden cardiac death in the young. Read More

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February 2021

A Bioinformatics Analysis of the Potential Roles of Aquaporin 4 in Human Brain Tumors: An Immune-Related Process.

Front Pharmacol 2021 25;12:692175. Epub 2021 May 25.

Department of Neurosurgery, The Third People's Hospital of Dalian, Dalian Medical University, Dalian, China.

Aquaporin 4 (AQP4) is an ubiquitously expressed membrane protein channel found in the central nervous system and mainly on astrocytes. Recent studies on AQP4 has implicated it in tumorigenesis. It is of interest to determine the potential value of AQP4 in identifying, guiding treatment and prognosticating various types of CNS cancers. Read More

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Overexpressed Na 1.7 Channels Confer Hyperexcitability to Trigeminal Sensory Neurons of Ca 2.1 Mutant Hemiplegic Migraine Mice.

Front Cell Neurosci 2021 25;15:640709. Epub 2021 May 25.

Department of Neuroscience, International School for Advanced Studies (SISSA), Trieste, Italy.

Trigeminal sensory neurons of transgenic knock-in (KI) mice expressing the R192Q missense mutation in the α1A subunit of neuronal voltage-gated Ca 2.1 Ca channels, which leads to familial hemiplegic migraine type 1 (FHM1) in patients, exhibit a hyperexcitability phenotype. Here, we show that the expression of Na 1. Read More

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Impaired Binding to Junctophilin2 and Nanostructural Alteration in CPVT Mutation.

Circ Res 2021 Jun 11. Epub 2021 Jun 11.

Inserm UMR-S 1180, FRANCE.

Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a rare disease, manifested by syncope or sudden death in children or young adults under stress conditions. Mutations in the Ca release channel/ryanodine receptor (RyR2) gene account for about 60% of the identified mutations. Recently, we found and described a mutation in RyR2 N-terminal domain, RyR2. Read More

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Advances in Lymphedema.

Circ Res 2021 Jun 10;128(12):2003-2016. Epub 2021 Jun 10.

Allan and Tina Neill Professor of Lymphatic Research and Medicine, Stanford University School of Medicine, Stanford, CA.

Lymphedema is a common, complex, and inexplicably underappreciated human disease. Despite a history of relative neglect by health care providers and by governmental health care agencies, the last decade has seen an explosive growth of insights into, and approaches to, the problem of human lymphedema. The current review highlights the significant advances that have occurred in the investigative and clinical approaches to lymphedema, particularly over the last decade. Read More

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Dexmedetomidine Inhibits ASIC Activity Activation of α Adrenergic Receptors in Rat Dorsal Root Ganglion Neurons.

Front Pharmacol 2021 24;12:685460. Epub 2021 May 24.

Research Center of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Science and Technology, Xianning, China.

Dexmedetomidine (DEX), a selective α adrenergic receptor (α-AR) agonist, has been shown to have peripheral analgesic effects in a variety of pain conditions. However, the precise molecular mechanisms have not yet been fully elucidated. Acid sensing ion channels (ASICs) are the major player in pain associated with tissue acidosis. Read More

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Role of TRPM2 in brain tumours and potential as a drug target.

Acta Pharmacol Sin 2021 Jun 9. Epub 2021 Jun 9.

Department of Surgery, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada.

Ion channels are ubiquitously expressed in almost all living cells, and are the third-largest category of drug targets, following enzymes and receptors. The transient receptor potential melastatin (TRPM) subfamily of ion channels are important to cell function and survival. Studies have shown upregulation of the TRPM family of ion channels in various brain tumours. Read More

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A high-throughput microfluidic bilayer co-culture platform to study endothelial-pericyte interactions.

Sci Rep 2021 Jun 9;11(1):12225. Epub 2021 Jun 9.

The Charles Stark Draper Laboratory Inc., 555 Technology Square, Cambridge, MA, 02139, USA.

Microphysiological organ-on-chip models offer the potential to improve the prediction of drug safety and efficacy through recapitulation of human physiological responses. The importance of including multiple cell types within tissue models has been well documented. However, the study of cell interactions in vitro can be limited by complexity of the tissue model and throughput of current culture systems. Read More

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Molecular Monitoring of Knockdown Resistance in Head Louse (Phthiraptera: Pediculidae) Populations in Iran.

J Med Entomol 2021 Jun 9. Epub 2021 Jun 9.

Cellular and Molecular Research Center, Research Institute on Cellular and Molecular Medicine, Urmia University of Medical Sciences, Urmia, Iran.

Knockdown resistance (kdr) is a common mechanism of insecticide resistance in head lice to the conventionally used pyrethroid pediculosis and can be the result of various amino acid substitutions within the voltage-sensitive sodium channel (VSSC). In this study, 54 sequences from varied specimens were investigated to monitor well-known resistance mutations and probable new mutations. The Pediculus humanus capitis de Geer specimens were collected from 13 provinces in Iran. Read More

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GeSn Gate-All-Around p-Channel Metal-Oxide-Semiconductor Field-Effect Transistors with Sub-3 nm Nanowire Width.

Nano Lett 2021 Jun 9. Epub 2021 Jun 9.

Department of Electrical and Computer Engineering, National University of Singapore, Singapore 117576, Singapore.

We demonstrate GeSn p-channel gate-all-around field-effect transistors (p-GAAFETs) with sub-3 nm nanowire width () on a GeSn-on-insulator (GeSnOI) substrate using a top-down fabrication process. Thanks to the excellent gate control by employing an aggressively scaled nanowire structure, GeSn p-GAAFETs exhibit a small subthreshold swing (SS) of 66 mV/decade, a decent on-current/off-current (/) ratio of ∼1.2 × 10, and a high-field effective hole mobility () of ∼115 cm/(V s). Read More

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Inhibition of Nav1.7 channel by a novel blocker QLS-81 for alleviation of neuropathic pain.

Acta Pharmacol Sin 2021 Jun 8. Epub 2021 Jun 8.

Department of Pharmacology, School of Pharmacy, Qingdao University, Qingdao, 266073, China.

Voltage-gated sodium channel Nav1.7 robustly expressed in peripheral nociceptive neurons has been considered as a therapeutic target for chronic pain, but there is no selective Nav1.7 inhibitor available for therapy of chronic pain. Read More

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Arrhythmogenic and antiarrhythmic actions of late sustained sodium current in the adult human heart.

Sci Rep 2021 Jun 8;11(1):12014. Epub 2021 Jun 8.

AnaBios Corporation, 3030 Bunker Hill St., Suite 312, San Diego, CA, 92109, USA.

Late sodium current (late INa) inhibition has been proposed to suppress the incidence of arrhythmias generated by pathological states or induced by drugs. However, the role of late INa in the human heart is still poorly understood. We therefore investigated the role of this conductance in arrhythmias using adult primary cardiomyocytes and tissues from donor hearts. Read More

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Enhanced responsivity and detectivity of fast WSe phototransistor using electrostatically tunable in-plane lateral p-n homojunction.

Nat Commun 2021 Jun 7;12(1):3336. Epub 2021 Jun 7.

Department of Electrical Engineering, IIT Bombay, Mumbai, India.

Layered transition metal dichalcogenides have shown tremendous potential for photodetection due to their non-zero direct bandgaps, high light absorption coefficients and carrier mobilities, and ability to form atomically sharp and defect-free heterointerfaces. A critical and fundamental bottleneck in the realization of high performance detectors is their trap-dependent photoresponse that trades off responsivity with speed. This work demonstrates a facile method of attenuating this trade-off by nearly 2x through integration of a lateral, in-plane, electrostatically tunable p-n homojunction with a conventional WSe phototransistor. Read More

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Conserved modules required for TRP function .

J Neurosci 2021 Jun 7. Epub 2021 Jun 7.

Department of Molecular, Cellular, and Developmental Biology and the Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA

TRP channels are broadly required in animals for sensory physiology. To provide insights into regulatory mechanisms, the structures of many TRPs have been solved. This has led to new models, some of which have been tested Here, using the classical TRP required for visual transduction, we uncovered structural requirements for channel function in photoreceptor cells. Read More

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Fluorescent toxins as ion channel activity sensors.

Methods Enzymol 2021 12;653:295-318. Epub 2021 Apr 12.

Department of Physiology & Membrane Biology, University of California, Davis, CA, United States; Department of Anesthesiology & Pain Medicine, University of California, Davis, CA, United States. Electronic address:

Voltage gated ion channels (VGICs) shape the electrical character of cells by undergoing structural changes in response to membrane depolarization. High-resolution techniques have provided a wealth of data on individual VGIC structures, but the conformational changes of endogenous channels in live cell membranes have remained unexplored. Here, we describe methods for imaging structural changes of voltage-gated K channels in living cells, using peptidyl toxins labeled with fluorophores that report specific protein conformations. Read More

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Monopolar Biphasic Stimulator with Discharge Function and Negative Level Shifter for Neuromodulation SoC Integration in Low-Voltage CMOS Process.

IEEE Trans Biomed Circuits Syst 2021 Jun 7;PP. Epub 2021 Jun 7.

A 16-channel monopolar biphasic stimulator chip with discharge function for biomedical applications is proposed and designed. To provide monopolar biphasic stimulus currents, the positive (6V) and the negative (-6V) voltage sources are supported to generate the desired current pulses of ±3 mA. The monopolar biphasic stimulator chip was fabricated in a 0. Read More

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Asymmetric Dressing of WSe with (Macro)molecular Switches: Fabrication of Quaternary-Responsive Transistors.

ACS Nano 2021 Jun 7. Epub 2021 Jun 7.

Université de Strasbourg, CNRS, ISIS, 8 allée Gaspard Monge, 67000 Strasbourg, France.

The forthcoming saturation of Moore's law has led to a strong demand for integrating analogue functionalities within semiconductor-based devices. As a step toward this goal, we fabricate quaternary-responsive WSe-based field-effect transistors (FETs) whose output current can be remotely and reversibly controlled by light, heat, and electric field. A photochromic silane-terminated spiropyran (SP) is chemisorbed on SiO forming a self-assembled monolayer (SAM) that can switch from the SP to the merocyanine (MC) form in response to UV illumination and switch back by either heat or visible illumination. Read More

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Promotion of Differentiating Bone Marrow Mesenchymal Stromal Cells (BMSCs) into Cardiomyocytes via HCN2 and HCN4 Cotransfection.

Biomed Res Int 2021 13;2021:5529276. Epub 2021 May 13.

Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, China.

Aim: Investigation of the influences HCN2 and HCN4 has on bone marrow mesenchymal stromal cells (BMSCs) on cardiomyocyte differentiation.

Methods: Miniature adult pigs were used for bone marrow extraction and isolation of BMSCs. The identification of these BMSCs was done by using flow cytometry for the detection of expressed surface antigens CD45, CD11B, CD44, and CD90. Read More

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Dynamic interaction of fluoroquinolones with magnesium ions monitored using bacterial outer membrane nanopores.

Chem Sci 2020 Aug 31;11(38):10344-10353. Epub 2020 Aug 31.

Department of Life Sciences and Chemistry, Jacobs University Bremen 28759 Bremen Germany.

Divalent ions are known to have a severe effect on the translocation of several antibiotic molecules into (pathogenic) bacteria. In the present study we have investigated the effect of divalent ions on the permeability of norfloxacin across the major outer membrane channels from (OmpF and OmpC) and (Omp35 and Omp36) at the single channel level. To understand the rate limiting steps in permeation, we reconstituted single porins into planar lipid bilayers and analyzed the ion current fluctuations caused in the presence of norfloxacin. Read More

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Differential Functional Changes of Nav1.2 Channel Causing -Related Epilepsy and Status Epilepticus During Slow Sleep.

Front Neurol 2021 19;12:653517. Epub 2021 May 19.

Pediatric Department, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Nav1.2 encoded by the gene is a brain-expressed voltage-gated sodium channel known to be associated with neurodevelopment disorders ranging from benign familial neonatal infantile seizures (BFIS) to developmental and epileptic encephalopathy (DEE) and autism spectrum disorder. Interestingly, status epilepticus during slow sleep (ESES), which aggravates cognitive impairment, has been found in -related epilepsy. Read More

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Capsaicin and Proton Differently Modulate Activation Kinetics of Mouse Transient Receptor Potential Vanilloid-1 Channel Induced by Depolarization.

Front Pharmacol 2021 20;12:672157. Epub 2021 May 20.

Division of Molecular Pharmacology and Cell Biophysics, Department of Disease Management Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan.

The transient receptor potential vanilloid type 1 (TRPV1) channel is a non-selective cation channel expressed with transient receptor potential ankyrin type 1 (TRPA1) in small and medial size neurons of the dorsal root ganglions and trigeminal ganglions. TRPV1 is activated by capsaicin, thermal stimuli higher than 43°C, mechanical stress, and protons (H). Although the TRPV1 channel does not have positively charged residues at regular intervals on its transmembrane segments, alterations in membrane potential also affect the state of TRPV1 channel. Read More

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Adaptive convolutional neural networks for accelerating magnetic resonance imaging via k-space data interpolation.

Med Image Anal 2021 May 16;72:102098. Epub 2021 May 16.

Center for Biomedical Image Computing and Analytics (CBICA), Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address:

Deep learning in k-space has demonstrated great potential for image reconstruction from undersampled k-space data in fast magnetic resonance imaging (MRI). However, existing deep learning-based image reconstruction methods typically apply weight-sharing convolutional neural networks (CNNs) to k-space data without taking into consideration the k-space data's spatial frequency properties, leading to ineffective learning of the image reconstruction models. Moreover, complementary information of spatially adjacent slices is often ignored in existing deep learning methods. Read More

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Cystic fibrosis.

Lancet 2021 Jun;397(10290):2195-2211

National Heart and Lung Institute, Imperial College London, London, UK; Royal Brompton and Harefield, Guy's and St Thomas' NHS Foundation Trust, London, UK. Electronic address:

Cystic fibrosis is a monogenic disease considered to affect at least 100 000 people worldwide. Mutations in CFTR, the gene encoding the epithelial ion channel that normally transports chloride and bicarbonate, lead to impaired mucus hydration and clearance. Classical cystic fibrosis is thus characterised by chronic pulmonary infection and inflammation, pancreatic exocrine insufficiency, male infertility, and might include several comorbidities such as cystic fibrosis-related diabetes or cystic fibrosis liver disease. Read More

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Functional expression of TMEM16A in taste bud cells.

J Physiol 2021 Jun 5. Epub 2021 Jun 5.

Neurobiology Group, SISSA, Scuola Internazionale Superiore di Studi Avanzati, Trieste, 34136, Italy.

Key Points: Taste transduction occurs in taste buds in the tongue epithelium The Ca -activated Cl channels TMEM16A and TMEM16B play relevant physiological roles in several sensory systems Here, we report that TMEM16A, but not TMEM16B, is expressed in the apical part of taste buds Large Ca -activated Cl currents blocked by Ani-9, a selective inhibitor of TMEM16A, are measured in type I, but not in type II or III taste cells ATP indirectly activates Ca -activated Cl currents in type I cells through TMEM16A channels These results indicate that TMEM16A is functional in type I taste cells and contribute to understanding the largely unknown physiological roles of these cells ABSTRACT: The Ca -activated Cl channels TMEM16A and TMEM16B have relevant roles in many physiological processes including neuronal excitability and regulation of Cl homeostasis. Here, we examined the presence of Ca -activated Cl channels in taste cells of mouse vallate papillae by using immunohistochemistry and electrophysiological recordings. By immunohistochemistry we showed that only TMEM16A, and not TMEM16B, was expressed in taste bud cells where it largely co-localized with the inwardly rectifying K channel KNCJ1 in the apical part of type I cells. Read More

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Ultrasound-gated computed tomography coronary angiography (CTCA): Development of ultrasound transducers with improved CT compatibility.

Med Phys 2021 Jun 4. Epub 2021 Jun 4.

Department of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Purpose: Cardiovascular disease (CVD) is a leading cause of death worldwide, with coronary artery disease (CAD) accounting for nearly half of all CVD deaths. The current gold standard for CAD diagnosis is catheter coronary angiography (CCA), an invasive, expensive procedure. Computed tomography coronary angiography (CTCA) represents an attractive non-invasive alternative to CCA, however, CTCA requires gated acquisition of CT data during periods of minimal cardiac motion (quiescent periods) to avoid non-diagnostic scans. Read More

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