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Highly sensitive magnetic relaxation sensing method for aflatoxin B1 detection based on Au NP-assisted triple self-assembly cascade signal amplification.

Biosens Bioelectron 2021 Jul 9;192:113489. Epub 2021 Jul 9.

Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science/Key Laboratory of Agro-Products Quality and Safety of MOA, Beijing, 100081, PR China. Electronic address:

Highly sensitive detection of aflatoxin B (AFB) is of great significance because of its high toxicity and carcinogenesis. We propose a magnetic relaxation sensing method based on gold nanoparticles (Au NPs)-assisted triple self-assembly cascade signal amplification for highly sensitive detection of AFB. Both AFB antibody and initiator DNA (iDNA) are labeled on Au NPs to form Ab-Au-iDNA probe. Read More

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Insights for applying erythrosine B as a green fluorescence probe for estimation of anticancer tamoxifen and its analog; clomiphene in nanogram concentration.

Authors:
M M Tolba M M Salim

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 12;263:120156. Epub 2021 Jul 12.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University - Egypt, New Damietta 34511, Egypt. Electronic address:

The growth of tumor tissue is extremely pervasive among post-menopausal women. Commonly, from the clinical application, adjuvant selective estrogen receptor modulators such as tamoxifen are prescribed for prohibiting metastatic breast cancer, while its analog, clomiphene, is used to treat infertility in women. Lately, the significance of green chemistry on our environment was through reducing the influence of hazardous chemicals. Read More

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FTIR based approach to study EnaC mechanosensory functions.

Prog Biophys Mol Biol 2021 Jul 19. Epub 2021 Jul 19.

Department of Human Genetics & Molecular Medicine, Central University of Punjab, Bathinda, 151401, India.

The pulmonary epithelial sodium ion channel (ENaC) is gaining importance for its sodium gating and mechanosensitive roles. The mechano functional studies on ENaC suggest direct molecular interactions between the ENaC protein with cytoskeleton microtubules and other extracellular matrix components. Also, in few mechanotransduction studies, ENaC was shown to respond both to membrane stretch as well as cell volume changes. Read More

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Effects of Rebamipide on Differences in Power and Axis of Corneal Astigmatism Between Two Intra-patient Keratometric Measurements in Dry Eyes.

Ophthalmol Ther 2021 Jul 22. Epub 2021 Jul 22.

Department of Ophthalmology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa, Yokohama, 236-0004, Kanagawa, Japan.

Introduction: This study investigated the effect of rebamipide on discrepancies in the power and axis of astigmatism between two intra-patient keratometric measurements in patients with dry eyes.

Methods: Fifty-eight dry eyes (with a short tear breakup time [TBUT] of less than 5 s) were analyzed. Patients with dry eye were treated with 2% rebamipide ophthalmic suspension (group R) or Mytear artificial tear ophthalmic solution (group M) for 4 weeks. Read More

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The integrity and barrier function of porcine vocal fold epithelium: its susceptibility to damage by deoxycholic acid compared with pepsin.

Eur Arch Otorhinolaryngol 2021 Jul 22. Epub 2021 Jul 22.

The Department of Otolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China.

Purpose: In this study, we aim to systematically evaluate the damaging role of gastric (pepsin and acid) and duodenal ingredients (bile acids) on vocal fold epithelium in excised porcine larynges.

Methods: Fresh ex vivo porcine larynges were exposed to one of five experimental conditions for 1 h. These conditions will be referred to as alkaline deoxycholic acid, acidic pepsin, acid pH3 only, acid pH5 only, and control, respectively. Read More

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Size-Selective VAILase Proteolysis Provides Dynamic Insights into Protein Structures.

Anal Chem 2021 Jul 22. Epub 2021 Jul 22.

CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Monitoring the dynamic alterations of protein structures within an aqueous solution remains enormously challenging. In this study, we describe a size-selective VAILase proteolysis (SVP)-mass spectrometry (MS) strategy to probe the protein structure changes without strict control of the proteolysis kinetics. The unique conformation selectivity of SVP depends on the uniform nano-sized entrance pores of the VAILase hexameric cage as well as the six inherent molecular rulers in the VAILase-substrate recognition and cleavage. Read More

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Volumetric coronary endothelial function assessment: a feasibility study exploiting stack-of-stars 3D cine MRI and image-based respiratory self-gating.

NMR Biomed 2021 Jul 21:e4589. Epub 2021 Jul 21.

Russel H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University, Baltimore, Maryland, USA.

Abnormal coronary endothelial function (CEF), manifesting as depressed vasoreactive responses to endothelial-specific stressors, occurs early in atherosclerosis, independently predicts cardiovascular events, and responds to cardioprotective interventions. CEF is spatially heterogeneous along a coronary artery in patients with atherosclerosis, and thus recently developed and tested non-invasive 2D MRI techniques to measure CEF may not capture the extent of changes in CEF in a given coronary artery. The purpose of this study was to develop and test the first volumetric coronary 3D MRI cine method for assessing CEF along the proximal and mid-coronary arteries with isotropic spatial resolution and in free-breathing. Read More

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Single-Molecule Techniques to Study Chromatin.

Front Cell Dev Biol 2021 5;9:699771. Epub 2021 Jul 5.

Chromosome Dynamics and Genome Stability, Institute of Epigenetics and Stem Cells, Helmholtz Zentrum München, Munich, Germany.

Besides the basic organization in nucleosome core particles (NCPs), eukaryotic chromatin is further packed through interactions with numerous protein complexes including transcription factors, chromatin remodeling and modifying enzymes. This nucleoprotein complex provides the template for many important biological processes, such as DNA replication, transcription, and DNA repair. Thus, to understand the molecular basis of these DNA transactions, it is critical to define individual changes of the chromatin structure at precise genomic regions where these machineries assemble and drive biological reactions. Read More

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Constructing a Facile Biocomputing Platform Based on Smart Supramolecular Hydrogel Film Electrodes with Immobilized Enzymes and Gold Nanoclusters.

ACS Appl Mater Interfaces 2021 Jul 21. Epub 2021 Jul 21.

Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, People's Republic of China.

Herein, fluorescent gold nanoclusters (AuNCs) and horseradish peroxidase (HRP) were simultaneously embedded into self-assembled dipeptide supramolecular films of -fluorenylmethoxycarbonyl diphenylalanine (Fmoc-FF) on the surface of ITO electrodes (Fmoc-FF/AuNCs/HRP) by using a simple single-step process. In the films, both the fluorescence property of AuNCs and the bioelectrocatalytic property of HRP were well maintained and could be reversibly regulated by pH-sensitive structural changes in the Fmoc-FF hydrogel films. Cu(II)/EDTA in the solution could lead to the aggregation/disaggregation of AuNCs and further quenching/dequenching the fluorescence signal from the films. Read More

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Effects of Maryland's Global Budget Revenue Model on Emergency Department Utilization and Revisits.

Acad Emerg Med 2021 Jul 20. Epub 2021 Jul 20.

US Acute Care Solutions, Canton.

Background: In 2014, Maryland (MD) implemented a "global budget revenue" (GBR) program that prospectively sets hospital budgets. This program introduced incentives for hospitals to tightly control volume and meet budget targets. We examine GBR's effects on emergency department (ED) visits, admissions, and returns. Read More

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ASSESSMENT OF THE INTESTINAL MICROBIOTA IN ADULTS WITH EROSIVE ESOPHAGITIS.

Arq Gastroenterol 2021 Jul 16. Epub 2021 Jul 16.

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brasil.

Background: The intestinal microbiota influences the appropriate function of the gastrointestinal tract. Intestinal dysbiosis may be associated with a higher risk of esophageal lesions, mainly due to changes in gastroesophageal motility patterns, elevation of intra-abdominal pressure, and increased frequency of transient relaxation of the lower esophageal sphincter.

Objective: The aim of this study was to evaluate the intestinal microbiota in individuals with erosive esophagitis and in healthy individuals using metagenomics. Read More

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Complexation of -Functionalized Cyclams with Copper(II) and Zinc(II): Similarities and Changes When Compared to Parent Cyclam Analogues.

Inorg Chem 2021 Jul 21. Epub 2021 Jul 21.

University Brest, UMR CNRS 6521 CEMCA, 6 avenue Victor le Gorgeu, 29238 Brest, France.

Herein, we report a comprehensive coordination study of the previously reported ligands , , , , and and of their -functional analogues, , , , , and . This group of ligands includes , cross-bridged , their di- or tetramethylated derivatives, and the analogues bearing an additional hydroxyethyl group on one β-N position of the ring. The Cu(II) and Zn(II) complexes of these macrocycles have been highlighted previously for the biological interest, but the details of their structures in the solid state and in solution remained largely unexplored. Read More

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Backbone resonance assignments of the A2 domain of mouse von Willebrand factor.

Biomol NMR Assign 2021 Jul 20. Epub 2021 Jul 20.

Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku Katsura, Nishikyo-Ku, Kyoto, 615-8510, Japan.

von Willebrand factor (vWF) is an adhesive plasma protein that is important for platelet adhesion in normal hemostasis in response to vascular injury. Although large vWF multimers are released from storage granules of platelets and (sub-)endothelial cells in response to hemostatic stimuli, for normal physiological function, vWF multimers are required to be cleaved into smaller multimeric forms. The plasma metalloproteinase ADAMTS13 specifically cleaves the peptide bond located in the middle of the A2 domain of vWF (vWF-A2), but the cleavage site is buried inside the structure of vWF and is difficult to access in the absence of elevated flow shear stress. Read More

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Impact of GaAs(100) surface preparation on EQE of AZO/AlO/p-GaAs photovoltaic structures.

Beilstein J Nanotechnol 2021 28;12:578-592. Epub 2021 Jun 28.

Institute of Physics, Polish Academy of Sciences, Aleja Lotników 32/46, PL-02668 Warsaw, Poland.

In order to effectively utilize the photovoltaic properties of gallium arsenide, its surface/interface needs to be properly prepared. In the experiments described here we examined eight different paths of GaAs surface treatment (cleaning, etching, passivation) which resulted in different external quantum efficiency (EQE) values of the tested photovoltaic (PV) cells. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) examinations were conducted to obtain structural details of the devices. Read More

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Short-term high fat diet alters genes associated with metabolic and vascular dysfunction during adolescence in rats: a pilot study.

PeerJ 2021 9;9:e11714. Epub 2021 Jul 9.

College of Health Solutions & School of Life Sciences, Arizona State University, Tempe, AZ, USA.

Background: Diet-induced metabolic dysfunction precedes multiple disease states including diabetes, heart disease, and vascular dysfunction. The critical role of the vasculature in disease progression is established, yet the details of how gene expression changes in early cardiovascular disease remain an enigma. The objective of the current pilot project was to evaluate whether a quantitative assessment of gene expression within the aorta of six-week old healthy male Sprague-Dawley rats compared to those exhibiting symptoms of metabolic dysfunction could reveal potential mediators of vascular dysfunction. Read More

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Liver Protection Mechanism and Absorption Promotion Technology of Silybin Based on Intelligent Medical Analysis.

J Healthc Eng 2021 3;2021:9968016. Epub 2021 Jul 3.

Zhucheng People's Hospital, Zhucheng 262200, Shandong, China.

With the continuous popularization of smart medicine, the protective effect of silibinin in the liver has attracted much attention. This study mainly explores the liver protection mechanism and absorption promotion technology of silybin based on intelligent medical analysis. Refining of silibinin: accurately weigh 1. Read More

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The Effects of Fruit Extract on Glycemic Control Parameters in Patients with Type 2 Diabetes Mellitus: A Randomized Controlled Clinical Trial.

Evid Based Complement Alternat Med 2021 5;2021:5583691. Epub 2021 Jul 5.

Department of Epidemiology and Statistics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Bunge fruits have been utilized in traditional medicine to control diabetes mellitus (DM). However, no clinical survey has been done in this regard. This study was conducted to clinically evaluate the effects of fruit extract of this plant in improving glycemic control indices in patients with type 2 DM (T2DM). Read More

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Reward-specific satiety affects subjective value signals in orbitofrontal cortex during multicomponent economic choice.

Proc Natl Acad Sci U S A 2021 Jul;118(30)

Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom

Sensitivity to satiety constitutes a basic requirement for neuronal coding of subjective reward value. Satiety from natural ongoing consumption affects reward functions in learning and approach behavior. More specifically, satiety reduces the subjective economic value of individual rewards during choice between options that typically contain multiple reward components. Read More

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Effects of Vanadium Inhalation and Sweetened Beverage Ingestion in Mice: Morphological and Biochemical Changes in the Liver.

Int J Toxicol 2021 Jul 20:10915818211030858. Epub 2021 Jul 20.

Departamento de Biología Celular y Tisular, Facultad de Medicina, 61589Universidad Nacional Autonoma de México (UNAM), Mexico City, Mexico.

The aim of this report was to evaluate the morphological and biochemical changes in the liver by the inhalation of vanadium and consumption of sweetened beverages in a subchronic murine model. Forty CD-1 male mice were randomly divided into four groups: control, vanadium (V), sucrose 30% (S), and vanadium-sucrose (V + S). V was inhaled (1. Read More

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G-quadruplex DNAzyme as peroxidase mimetic in a colorimetric biosensor for ultrasensitive and selective detection of trace tetracyclines in foods.

Food Chem 2021 Jul 9;366:130560. Epub 2021 Jul 9.

School of Liquor and Food Engineering, Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy; Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Huaxi District, Guiyang 550025, China.. Electronic address:

The colorimetric method can determine the initial results even by the naked eyes, but its main challenge for antibiotics detection in food at present is the relatively low sensitivity. Herein, an ultrasensitive colorimetric biosensor based on G-quadruplex DNAzyme was firstly proposed for the rapid detection of trace tetracycline antibiotics like tetracycline, oxytetracycline, chlortetracycline and doxycycline. DNAzyme composed of hemin and G-quadruplex has peroxidase-like activity, and tetracyclines can combine with hemin to form a stable complex and reduce catalytic activity, making the color of solution changes from yellow to green. Read More

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Effects of antiepileptic drugs on ovaries of female Wistar rats.

Biotech Histochem 2021 Jul 19:1-8. Epub 2021 Jul 19.

Department of Medical Pharmacology, Faculty of Medicine, Inonu University, Malatya, Turkey.

Valproate (VPA) induced changes in ovarian morphology are observed in humans with epilepsy and in non-epileptic animals. The effects of lamotrigine (LTG) on female reproduction is not well known. We investigated whether LTG might be a safer drug for use with patients of reproductive age. Read More

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Endophytic Microbiome Responses to Sulfur Availability in   (L.).

Int J Mol Sci 2021 Jul 2;22(13). Epub 2021 Jul 2.

Department of Agronomy, Food, Natural Resources, Animals and Environment-DAFNAE, Agripolis, University of Padova, 35020 Legnaro, Italy.

Sulfur is an essential plant macronutrient, and its adequate supply allows an efficient root storage and sugar extractability in sugar beets (  L.). In this study, we investigated the effect of changes in sulfur availability on the endophytic community structure of sugar beets. Read More

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Regenerative Effects of Hypoxia Primed Flowable Placental Formulation in Muscle and Dermal Injury.

Int J Mol Sci 2021 Jul 1;22(13). Epub 2021 Jul 1.

Smith & Nephew Plc., Columbia, MD 21046, USA.

The placental tissue, due to its angiogenic, anti-inflammatory, antioxidative, antimicrobial, and anti-fibrotic properties, has become a compelling source towards a solution for several indications in regenerative medicine. However, methods to enhance and capture the therapeutic properties with formulations that can further the applications of viable placental tissue have not been explored. In this study, we investigated the regenerative effects of a hypoxia primed flowable placental formulation (FPF), composed of amnion/chorion and umbilical tissue, in two in vivo injury models. Read More

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Explanation of the Formation of Complexes between Representatives of Oxazolidinones and HDAS-β-CD Using Molecular Modeling as a Complementary Technique to cEKC and NMR.

Int J Mol Sci 2021 Jul 1;22(13). Epub 2021 Jul 1.

Department of Synthetic Drugs, National Medicines Institute, Chełmska 30/34, 00-725 Warsaw, Poland.

Molecular modeling (MM) results for tedizolid and radezolid with heptakis-(2,3-diacetyl-6-sulfo)-β-cyclodextrin (HDAS-β-CD) are presented and compared with the results previously obtained for linezolid and sutezolid. The mechanism of interaction of chiral oxazolidinone ligands belonging to a new class of antibacterial agents, such as linezolid, tedizolid, radezolid, and sutezolid, with HDAS-β-CD based on capillary electrokinetic chromatography (cEKC), nuclear magnetic resonance (NMR) spectroscopy, and MM methods was described. Principles of chiral separation of oxazolidinone analogues using charged single isomer derivatives of cyclodextrin by the cEKC method were presented, including the selection of the optimal chiral selector and separation conditions, complex stoichiometry, and binding constants, which provided a comprehensive basis for MM studies. Read More

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Optimisation of Fixed-Outlet and Flow-Modulated Pressure Reduction Measures in Looped Water Distribution Networks Constrained by Fire-Fighting Capacity Requirements.

Int J Environ Res Public Health 2021 Jul 2;18(13). Epub 2021 Jul 2.

Department of Civil and Environmental Engineering, Norwegian University of Science and Technology, S.P. Andersens veg 5, 7031 Trondheim, Norway.

Pressure management is a pivotal component when reducing leakages from water distribution networks, and can be achieved by sub-dividing existing networks into partitions where the pressure can be reduced effectively. There is a need to develop methods that aid in the identification of cost-effective partitions for pressure reduction, while simultaneously verifying that the topological changes entailed in these solutions do not compromise reliability and (fire-fighting) capacity requirements, especially in systems where the capacity is ensured through looped networks. This paper presents a method that can be used to this end, in which a novel combination of hydraulic simulations and graph theory is used to determine the maximal potential for (dynamic and static) pressure reduction, and this is used as a constraint for multi-objective optimization of pressure reduction measures. Read More

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Thermoresponsivity of poly(N-isopropylacrylamide) microgels in water-trehalose solution and its relation to protein behavior.

J Colloid Interface Sci 2021 Jul 8;604:705-718. Epub 2021 Jul 8.

Dipartimento di Fisica e Geologia, Università di Perugia, I-06123 Perugia, Italy. Electronic address:

Hypotheses: Additives are commonly used to tune macromolecular conformational transitions. Among additives, trehalose is an excellent bioprotectant and among responsive polymers, PNIPAM is the most studied material. Nevertheless, their interaction mechanism so far has only been hinted without direct investigation, and, crucially, never elucidated in comparison to proteins. Read More

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Calcium mediated DNA binding in non-lamellar structures formed by DOPG/glycerol monooleate.

Chem Phys Lipids 2021 Jul 16;239:105118. Epub 2021 Jul 16.

Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32, Bratislava, Slovakia. Electronic address:

In order to test an encapsulation method of short fragmented DNA (∼ 20-300 bp), we study the solubilisation in 150 mM solution of NaCl of a cubic phase formed by glycerol monooleate (GMO) with negatively charged dioleoylphosphatidylglycerol (DOPG) up to the level of unilamellar vesicles and, subsequently, the restoration of the cubic phase using Ca cations. We performed small angle X-ray and neutron scattering (SAXS and SANS) to follow structural changes in DOPG/GMO mixtures induced by increasing DOPG content. The cubic phase (Pn3m space group) is preserved up to ∼ 11 mol% of DOPG in DOPG/GMO. Read More

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Rheological and NMR Studies of Cellulose Dissolution in the Ionic Liquid BmimAc.

J Phys Chem B 2021 Jul 19. Epub 2021 Jul 19.

School of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, U.K.

Solutions of two types of cellulose in the ionic liquid 1-butyl-3-methyl-imidazolium acetate (BmimAc) have been analyzed using rheology and fast-field cycling nuclear magnetic resonance (NMR) spectroscopy, in order to analyze the macroscopic (bulk) and microscopic environments, respectively. The degree of polymerization (DP) was observed to have a significant effect on both the overlap (*) and entanglement () concentrations and the intrinsic viscosity ([η]). For microcrystalline cellulose (MCC)/BmimAc solutions, [η] = 116 mL g, which is comparable to that of MCC/1-ethyl-3-methyl-imidazolium acetate (EmimAc) solutions, while [η] = 350 mL g for the commercial cellulose (higher DP). Read More

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Morphine Prevents Ischemia/Reperfusion-Induced Myocardial Mitochondrial Damage by Activating δ-opioid Receptor/EGFR/ROS Pathway.

Cardiovasc Drugs Ther 2021 Jul 19. Epub 2021 Jul 19.

School of Public Health, North China University of Science and Technology, 21 Bohai Avenue, Caofeidian District, Tangshan, 063000, Hebei, China.

Objective: The purpose of this study was to determine whether the epidermal growth factor receptor (EGFR), which is a classical receptor tyrosine kinase, is involved in the protective effect of morphine against ischemia/reperfusion (I/R)-induced myocardial mitochondrial damage.

Methods: Isolated rats hearts were subjected to global ischemia followed by reperfusion. Cardiac H9c2 cells were exposed to a simulated ischemia solution followed by Tyrode's solution to induce hypoxia/reoxygenation (H/R) injury. Read More

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Understanding Organismal Capacity to Respond to Anthropogenic Change: Barriers and Solutions.

Integr Comp Biol 2021 Jul 19. Epub 2021 Jul 19.

Biology Department, New Mexico State University, Las Cruces, NM, 88003, USA.

Global environmental changes induced by human activities are forcing organisms to respond at an unprecedented pace. At present we have only a limited understanding of why some species possess the capacity to respond to these changes while others do not. We introduce the concept of multidimensional phenospace as an organizing construct to understanding organismal evolutionary responses to environmental change. Read More

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