933 results match your criteria challenging non-specific


High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion.

Elife 2021 Jun 8;10. Epub 2021 Jun 8.

Vollum Institute, Oregon Health and Science University, Portland, United States.

Precise and efficient insertion of large DNA fragments into somatic cells using gene editing technologies to label or modify endogenous proteins remains challenging. Non-specific insertions/deletions (INDELs) resulting from the non-homologous end joining pathway make the process error-prone. Further, the insert is not readily removable. Read More

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Treatment-resistant hypertension in a post-transplant patient with cystic fibrosis: a rare case of phaeochromocytoma.

Endocrinol Diabetes Metab Case Rep 2021 Jun 1;2021. Epub 2021 Jun 1.

Department of EndocrinologyMater Misericordiae University Hospital, Dublin, Ireland.

Summary: Phaeochromocytoma is a rare catecholamine-producing tumour. We present the case of phaeochromocytoma in a young man with a background history of a double-lung transplant for cystic fibrosis (CF). Clinical case: A 25-year-old man, with a background history of CF, CF-related diabetes (CFRD) and a double-lung transplant in 2012 was presented to the emergency department with crampy abdominal pain, nausea and vomiting. Read More

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Pneumopericardium secondary to oesophageal cancer presenting as myocardial infarction: a case report.

Respirol Case Rep 2021 Jul 26;9(7):e00791. Epub 2021 May 26.

Division of Thoracic Surgery, Department of Surgery National Taiwan University Hospital Yun-Lin Branch Douliu Taiwan.

The diagnosis of pneumopericardium can be challenging, as the symptoms are sometimes non-specific and can mimic those of other diseases. Especially, focal pneumopericardium presenting with regional ST-segment elevation on the electrocardiogram can be difficult to diagnose. Here, we present the case of a patient with pneumopericardium secondary to oesophageal cancer that was first diagnosed with community-acquired pneumonia and subsequently classified with acute coronary syndrome after an episode of cardiac arrest. Read More

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Unusual clinical manifestation and challenging serological interpretation of syphilis: insights from a case report.

BMC Infect Dis 2021 Jun 2;21(1):521. Epub 2021 Jun 2.

STI/HIV Unit, San Gallicano Dermatological Institute IRCCS, Rome, Italy.

Background: The clinical manifestations of recent syphilis can be variable, with typical and atypical patterns. Several conditions may cause atypical clinical aspects, including human immunodeficiency virus (HIV) co-infection. Besides the clinical features, co-infections may completely alter syphilis serological tests, causing interpretative difficulties and diagnostic delays. Read More

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Early-Onset Alcohol Dependence and Multiple Sclerosis: Diagnostic Challenges.

Int J Environ Res Public Health 2021 May 24;18(11). Epub 2021 May 24.

Section of Neurosciences, Department "GF. Ingrassia", University of Catania, Via S. Sofia, 78, 95123 Catania, Italy.

Multiple sclerosis (MS) is an inflammatory demyelinating disorder characterized by the progressive disruption of the myelin sheath around the nerve fibres. The early initiation of disease-modifying treatments is crucial for preventing disease progression and neurological damage. Unfortunately, a diagnostic delay of several years is not uncommon, particularly in the presence of physical and mental comorbidities. Read More

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An Editing-Site-Specific PCR Method for Detection and Quantification of -Edited Rice.

Foods 2021 May 27;10(6). Epub 2021 May 27.

Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.

Genome-edited plants created by genome editing technology have been approved for commercialization. Due to molecular characteristics that differ from classic genetically modified organisms (GMOs), establishing regulation-compliant analytical methods for identification and quantification of genome-edited plants has always been regarded as a challenging task. An editing-site-specific PCR method was developed based on the unique edited sequence in -edited rice plants. Read More

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Latest update on chemokine receptors as therapeutic targets.

Biochem Soc Trans 2021 Jun 1. Epub 2021 Jun 1.

School of Pharmacy, University of East Anglia, Norwich, U.K.

The chemokine system plays a fundamental role in a diverse range of physiological processes, such as homeostasis and immune responses. Dysregulation in the chemokine system has been linked to inflammatory diseases and cancer, which renders chemokine receptors to be considered as therapeutic targets. In the past two decades, around 45 drugs targeting chemokine receptors have been developed, yet only three are clinically approved. Read More

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Adrenal schwannoma: A case report of an unusual incidentaloma.

Int J Surg Case Rep 2021 May 26;83:106018. Epub 2021 May 26.

Department of Urology and Kidney Transplant Surgery, Tribhuvan University Teaching Hospital, Kathmandu, Nepal. Electronic address:

Introduction And Importance: Adrenal schwannomas are extremely rare tumors often misdiagnosed. The patients are usually asymptomatic while some present with non-specific abdominal pain. Only a few cases are reported to date. Read More

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F-FDG PET/CT Manifestations of IgG4-related Disease.

Br J Radiol 2021 May 28:20210105. Epub 2021 May 28.

Department of Nuclear Medicine and Molecular Imaging, Singapore General Hospital, Singapore, Singapore.

Immunoglobulin G4-related disease (IgG4-RD) was not recognised as a systemic condition until 2003, when extra pancreatic manifestations were identified in patients with autoimmune pancreatitis. Since then, IgG4-RD has been described to involve virtually every organ system. It is highly responsive to immunosuppressants but can have detrimental effects if left untreated. Read More

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Management of abdominal tuberculosis in a community-based hospital in a high-income developing country.

World J Emerg Surg 2021 May 26;16(1):25. Epub 2021 May 26.

Department of Surgery, College of Medicine and Health Sciences, UAE University, Al-Ain, UAE.

Background: The delayed diagnosis and management of abdominal tuberculosis increases its mortality. We aimed to study the clinical presentation, management, and outcome of patients who had abdominal tuberculosis and were treated at Al-Ain Hospital, Al-Ain City, United Arab Emirates.

Methods: All patients who had abdominal tuberculosis and were treated at Al-Ain Hospital between January 2011 and December 2018 were studied. Read More

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CRISPR/Cas12a-Assisted Ligation-Initiated Loop-Mediated Isothermal Amplification (CAL-LAMP) for Highly Specific Detection of microRNAs.

Anal Chem 2021 Jun 26;93(22):7942-7948. Epub 2021 May 26.

Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China.

Loop-mediated isothermal amplification (LAMP) has been increasingly applied in nucleic acid detection for clinical diagnosis and monitoring pathogenic microorganisms due to its isothermal nature and high sensitivity. However, the false-positive signal resulting from the non-specific amplification and the complexity of primer design are still technically challenging for wide applications. In this paper, we developed the CRISPR/Cas12a-assisted sequence-specific detection of LAMP products to eliminate the effect of non-specific amplification from primer dimers and spurious amplicons. Read More

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Chronic thromboembolic pulmonary hypertension following pulmonary embolism with a right ventricular thrombus: A report of two cases.

Pulm Circ 2021 Apr-Jun;11(2):20458940211013668. Epub 2021 May 13.

Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, PR China.

Right heart thrombus accompanied by chronic thromboembolic pulmonary hypertension is a rare entity. Right heart thrombus may develop in the peripheral veins or in situ within the right heart chambers. The diagnosis of right heart thrombus is challenging, since its symptoms are typically non-specific and its imaging features resemble those of cardiac masses. Read More

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[Thoracic Outlet Syndrome: Rare, Often Missed or Over-Diagnosed?]

Praxis (Bern 1994) 2021 ;110(7):391-396

Klinik für Thoraxchirurgie, Inselspital, Universitätsspital Bern, Bern.

Thoracic Outlet Syndrome: Rare, Often Missed or Over-Diagnosed? The thoracic outlet syndrome (TOS) presents with various symptoms caused by compression of the neurovascular bundle in the region of the upper thoracic aperture. Since the pathogenesis also determines the therapy of TOS, the classification according to the affected structure into neurogenic, venous and arterial TOS (nTOS, vTOS and aTOS) is useful. However, mixed forms are often to be assumed, which are then usually also classified under the term 'non-specific or disputed TOS' in the group of nTOS. Read More

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Gastrocolonic fistula: a rare complication of upper gastrointestinal surgery.

J Surg Case Rep 2021 May 14;2021(5):rjab181. Epub 2021 May 14.

Department of General Surgery, Monash Health, Clayton, Victoria, Australia.

A gastrocolonic fistula is a rare surgical presentation, typically in the setting of gastric or colonic malignancy. This report presents the first reported Australian case of a gastrocolonic fistula following upper gastrointestinal surgery. A middle-aged woman presented to emergency with a short history of severe abdominal pain, faeculent vomiting, profuse diarrhoea and weight loss. Read More

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Dietary supplementation of Bacillus velezensis B8 enhances immune response and resistance against Aeromonas veronii in grass carp.

Fish Shellfish Immunol 2021 May 17;115:14-21. Epub 2021 May 17.

College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi, 712100, China. Electronic address:

The heavy use of prophylactic antibiotics in aquaculture leads to elevated antibiotic residues, posing a huge hidden danger in aquaculture products and other natural aquatic environments. Therefore, this study aims to isolate probiotics that can replace antibiotics from the gut of grass carp for disease control. Bacillus velezensis B8 was isolated from the gut of grass carp and showed broad-spectrum antimicrobial activity against several fish pathogenic bacteria, including Aeromonas hydrophilis, Aeromonas veronii, Vibrio parahaemolyticus, Escherichia coli, Edwardsiella tarda and Vibrio mimicus. Read More

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Asymptomatic peritoneal tuberculosis in a patient with a non-remitting fever and isolated pulmonary symptoms: a case report and literature review.

Radiol Case Rep 2021 Jul 30;16(7):1679-1684. Epub 2021 Apr 30.

Royal Free London NHS Foundation Trust, Pond St, Hampstead, London NW3 2QG, UK.

Tuberculosis (TB) remains one of the leading causes of death globally. Although abdominal or peritoneal TB is a recognised site for extrapulmonary TB to manifest, the diagnosis is often delayed due to the non-specific nature of the presenting clinical features. We present the diagnostically challenging case of a 32-year-old patient with recurrent episodes of fever and a non-productive cough that was initially treated as community-acquired pneumonia with oral antibiotics. Read More

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Early diagnosis of brain tumours using a novel spectroscopic liquid biopsy.

Brain Commun 2021 30;3(2):fcab056. Epub 2021 Mar 30.

ClinSpec Diagnostics Limited, Royal College Building, Glasgow G1 1XW, UK.

Early diagnosis of brain tumours is challenging and a major unmet need. Patients with brain tumours most often present with non-specific symptoms more commonly associated with less serious diagnoses, making it difficult to determine which patients to prioritize for brain imaging. Delays in diagnosis affect timely access to treatment, with potential impacts on quality of life and survival. Read More

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Diagnostic Delay of Pulmonary Embolism in COVID-19 Patients.

Front Med (Lausanne) 2021 30;8:637375. Epub 2021 Apr 30.

Department of Internal Medicine, San Matteo Hospital Foundation, University of Pavia, Pavia, Italy.

Pulmonary embolism (PE) is a frequent, life-threatening COVID-19 complication, whose diagnosis can be challenging because of its non-specific symptoms. There are no studies assessing the impact of diagnostic delay on COVID-19 related PE. The aim of our exploratory study was to assess the diagnostic delay of PE in COVID-19 patients, and to identify potential associations between patient- or physician-related variables and the delay. Read More

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Relevant Journals for Identifying Implementation Science Articles: Results of an International Implementation Science Expert Survey.

Front Public Health 2021 30;9:639192. Epub 2021 Apr 30.

Department Public Health, Institute of Nursing Science, University of Basel, Basel, Switzerland.

In implementation science (IS), conducting well-targeted and reproducible literature searches is challenging due to non-specific and varying terminology that is fragmented over multiple disciplines. A list of journals that publish IS-relevant content for use in search strings can support this process. We conducted a cross-sectional online survey of 56 Australian, European, and North American IS experts to identify and prioritize relevant journals that publish IS articles. Read More

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Reliability of postural control during double-leg standing in subjects with nonspecific chronic low back pain: Dual-task paradigm and manipulated visual and somatosensory inputs.

J Bodyw Mov Ther 2021 Apr 17;26:49-56. Epub 2020 Sep 17.

Department of Physical Therapy, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Background: The aim of this study is to evaluate the test-retest reliability of the Center of Pressure (COP) parameters in quiet double-leg standing in subjects with Non-Specific Chronic Low Back Pain (NSCLBP) during dual taking associated with manipulated visual and somatosensory inputs.

Materials And Methods: In this observational cross-sectional study, the static balance of thirty NSCLBP patients were assessed during a double-leg stance by using the force platform. Subjects were accosted by manipulated somatosensory and visual inputs during dual taking in eight different conditions (with and without vibration, eyes-open and eyes-closed, and with and without auditory Stroop test). Read More

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A 25-Year-Old Man with Refractory Schizophrenia and Clozapine-Induced Myocarditis Diagnosed by Non-Invasive Cardiovascular Magnetic Resonance.

Am J Case Rep 2021 May 15;22:e930103. Epub 2021 May 15.

Department of Cardiology, Heart and Vascular Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.

BACKGROUND Clozapine, a second-generation antipsychotic, is often prescribed for refractory schizophrenia; however, it can cause life-threatening adverse events including agranulocytosis and myocarditis. Making the diagnosis of clozapine-induced myocarditis can be challenging given the non-specific presentation as well as risk involved in obtaining an endomyocardial biopsy. As clozapine-induced myocarditis carries a mortality risk of up to 30%, timely recognition, diagnosis, and management are vital. Read More

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PhyloPrimer: a taxon-specific oligonucleotide design platform.

PeerJ 2021 29;9:e11120. Epub 2021 Apr 29.

School of Biological Sciences, University of Bristol, Bristol, UK.

Many environmental and biomedical biomonitoring and detection studies aim to explore the presence of specific organisms or gene functionalities in microbiome samples. In such cases, when the study hypotheses can be answered with the exploration of a small number of genes, a targeted PCR-approach is appropriate. However, due to the complexity of environmental microbial communities, the design of specific primers is challenging and can lead to non-specific results. Read More

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Anterior Segment Findings in Ocular Myocysticercosis in Children: Is it non- specific?

Nepal J Ophthalmol 2021 Jan;13(25):141-145

Nirwana Netralaya, Athkhambhwa, Sasaram- 821115, Bihar, India.

Ocular cysticercosis is a preventable cause of blindness. Medical therapy has been recommended for the retro-orbital and extraocular muscle form. Surgical management is largely done in cases with conjunctival or lid affliction. Read More

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January 2021

Emergomycosis, an Emerging Systemic Mycosis in Immunocompromised Patients: Current Trends and Future Prospects.

Front Med (Lausanne) 2021 23;8:670731. Epub 2021 Apr 23.

Department of Microbiology, All India Institute of Medical Sciences, Jodhpur, India.

Recently, the global emergence of emergomycosis, a systemic fungal infection caused by a novel dimorphic fungus species has been observed among immunocompromised individuals. Though initially classified under the genus , a taxonomic revision in 2017 based on DNA sequence analyses placed five -like fungi under a separate genus . These include , and . Read More

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A Five-Year Review on Pediatric Foreign Body Aspiration.

Int Arch Otorhinolaryngol 2021 Apr 23;25(2):e193-e199. Epub 2020 Jun 23.

Department of Surgery, Otorhinolaryngology - Head and Neck Surgery Unit, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.

 Foreign body aspiration is a leading cause of accidental death in children. Clinical presentation varies from non-specific respiratory symptoms to respiratory failure making diagnosis challenging.  To review pediatric patients who underwent bronchoscopy due to suspicion of foreign body aspiration at a tertiary center in Malaysia. Read More

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Distinguishing coronavirus disease 2019 from influenza in children remains challenging.

New Microbes New Infect 2021 May 24;41:100888. Epub 2021 Apr 24.

University Tunis El Manar, Faculty of Medicine of Tunis, Tunisia.

Clinical descriptions about influenza-like illness in children seem non-specific during the co-circulation of SARS-CoV-2 and influenza. This paper aimed to summarize recent studies comparing clinical features and outcome, laboratory and radiological findings of COVID-19 patients with influenza patients in the paediatric population. Read More

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A Detailed Analysis of Clinical Features and Outcomes of Patients with Pyogenic Spondylodiscitis Presenting without Axial Back Pain.

Trop Med Infect Dis 2021 Apr 20;6(2). Epub 2021 Apr 20.

Department of Orthopaedics, A.O.U. "Vanvitelli" University Hospital, "Luigi Vanvitelli" University, Via del Sole 10, 80138 Naples, Italy.

Study Design: Retrospective analysis of a single institution prospective, longitudinal database of spinal pyogenic infections. Diagnosis of pyogenic spondylodiscitis (PS) can be challenging. Although presenting symptoms are often non-specific, acute non-remitting axial back pain is the most striking feature. Read More

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Diversity of Pathological Conditions Affecting Pituitary Stalk.

J Clin Med 2021 Apr 14;10(8). Epub 2021 Apr 14.

Department of Endocrinology, Faculty of Medicine, Medical College, Jagiellonian University, 31-008 Krakow, Poland.

Pituitary stalk lesions (PSL) are a very rare pathology. The majority of conditions affecting the infundibulum do not present with clinically apparent symptoms, what makes the diagnosis difficult. The recognition might be also complicated by the non-specific and transient characteristics of hormonal insufficiencies. Read More

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Primary Mucosal Melanoma Presenting with a Unilateral Nasal Obstruction of the Left Inferior Turbinate.

Medicina (Kaunas) 2021 Apr 8;57(4). Epub 2021 Apr 8.

Department of Health Sciences, University "Magna Graecia" of Catanzaro, 88100 Catanzaro, Italy.

We report the case of a primitive nasal melanoma in an 82-year-old patient, showing how this rare malignancy, with non-specific signs and symptoms, can represent a challenging diagnosis for the physician. A 82-year-old Caucasian patient presented for unilateral nasal obstruction and occasional epistaxis. Computerized tomography (CT) and magnetic resonance imaging (MRI) of the facial massif revealed turbinate hypertrophy and a polypoid phlogistic tissue isointense in T1 with an intermediate signal in T2 and Short-TI Inversion Recovery (STIR)-T2, occupying the middle meatus and the anterior upper and lower left meatus with partial obliteration of the ostium and the infundibulum of the maxillary sinus. Read More

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Splenic artery pseudoaneurysm: Challenges of non-invasive and endovascular diagnosis and management.

Radiol Case Rep 2021 Jun 10;16(6):1395-1399. Epub 2021 Apr 10.

Department of Radiology, Royal Free Hospital, Royal Free London NHS Foundation Trust, Pond Street, Hampstead, London, NW3 2QG, UK.

Splenic artery pseudoaneurysms (PAs) are uncommon and often occur as a complication of pancreatitis or trauma. Unlike true aneurysms, PAs are symptomatic in a majority of cases and patients can present with a constellation of non-specific symptoms. Diagnosis can be challenging due to variation in presenting features and mimicking pathologies. Read More

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