12,670 results match your criteria chains report


The accuracy of NMR protein structures in the Protein Data Bank.

Structure 2021 Jul 28. Epub 2021 Jul 28.

Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK. Electronic address:

The program ANSURR measures the accuracy of NMR structures by comparing rigidity obtained from experimental backbone chemical shifts and from structures. We report on ANSURR analysis of 7,000 PDB NMR ensembles within the Protein Data Bank, which can be found at ansurr.com. Read More

View Article and Full-Text PDF

First Report of Powdery Mildew Caused by Golovinomyces ambrosiae on Bidens pilosa in China.

Plant Dis 2021 Jul 30. Epub 2021 Jul 30.

Minjiang University, 26465, Institute of Oceanography, Fuzhou, Fujian, China;

Bidens pilosa L., (spanish needle), is a wild, flowering plant of Asteraceae, that is grown in gardens, fields, roadsides, and riverbanks in Fuzhou, China. It is also used in traditional folk medicine for a broad range of ailments in China. Read More

View Article and Full-Text PDF

Rapid Assembly of Infection-Resistant Coatings: Screening and Identification of Antimicrobial Peptides Works in Cooperation with an Antifouling Background.

ACS Appl Mater Interfaces 2021 Jul 30. Epub 2021 Jul 30.

Centre for Blood Research and Department of Pathology & Laboratory Medicine, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.

Bacterial adhesion and the succeeding biofilm formation onto surfaces are responsible for implant- and device-associated infections. Bifunctional coatings integrating both nonfouling components and antimicrobial peptides (AMPs) are a promising approach to develop potent antibiofilm coatings. However, the current approaches and chemistry for such coatings are time-consuming and dependent on substrates and involve a multistep process. Read More

View Article and Full-Text PDF

Aerogelation of Polymer-Coated Photoluminescent, Plasmonic, and Magnetic Nanoparticles for Biosensing Applications.

ACS Appl Nano Mater 2021 Jul 8;4(7):6678-6688. Epub 2021 Jul 8.

Institute of Physical Chemistry and Electrochemistry, Leibniz Universität Hannover, Callinstr. 3a, Hanover 30167, Germany.

Macroscopic materials with nanoscopic properties have recently been synthesized by self-assembling defined nanoparticles to form self-supported networks, so-called aerogels. Motivated by the promising properties of this class of materials, the search for versatile routes toward the controlled assembly of presynthesized nanoparticles into such ultralight macroscopic materials has become a great interest. Overcoating procedures of colloidal nanoparticles with polymers offer versatile means to produce aerogels from nanoparticles, regardless of their size, shape, or properties while retaining their original characteristics. Read More

View Article and Full-Text PDF

Light Chain Deposition Disease presenting as an Atrial Mass: A Case Report and Review of Literature.

Cardiovasc Pathol 2021 Jul 26:107368. Epub 2021 Jul 26.

Department of Pathology, Fairfield Medical Center, Lancaster, Ohio. Electronic address:

Light chain deposition disease (LCDD) also known as Non-Amyloidotic Immunoglobulin Deposition Disease (NIDD) is a rare systemic disorder due to the abnormal deposition of immunoglobulin in multiple organs caused by the clonal proliferation of B lymphocytes and plasma cells. Renal involvement is the most common with cardiac manifestations being the most common extra renal presentation of the disease. Renal involvement is not always associated with LCDD. Read More

View Article and Full-Text PDF

First Report of Fruit Rot of Grapes (Vitis vinifera) Caused by Cladosporium cladosporioides in Xinjiang, China.

Plant Dis 2021 Jul 28. Epub 2021 Jul 28.

Shihezi University, Plant protection, Shihezi, Xinjiang, China;

Xinjiang Province accounts for nearly 20% of the total grape (Vitis Vinifera L.), proles orientalis) (wine, table and raisin combined) production, being the largest production area in China. Fruit rot is the most common disease that impacts grape quality and yield. Read More

View Article and Full-Text PDF

Humoral response to neurofilaments and dipeptide repeats in ALS progression.

Ann Clin Transl Neurol 2021 Jul 27. Epub 2021 Jul 27.

Neurodegeneration Group, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, E1 2AT, United Kingdom.

Objective: To appraise the utility as biomarkers of blood antibodies and immune complexes to neurofilaments and dipeptide repeat proteins, the products of translation of the most common genetic mutation in amyotrophic lateral sclerosis (ALS).

Methods: Antibodies and immune complexes against neurofilament light, medium, heavy chains as well as poly-(GP)-(GR) dipeptide repeats were measured in blood samples from the ALS Biomarkers (n = 107) and the phenotype-genotype biomarker (n = 129) studies and in 140 healthy controls. Target analyte levels were studied longitudinally in 37 ALS cases. Read More

View Article and Full-Text PDF

An aptamer highly specific to cellulose enables the analysis of the association of cellulose with matrix cell wall polymers in vitro and in muro.

Plant J 2021 Jul 27. Epub 2021 Jul 27.

Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, DK-1871, Denmark.

The current toolbox of cell wall-directed molecular probes has been pivotal for advancing basic and application-oriented plant carbohydrate research; however, it still exhibits limitations regarding target diversity and specificity. Scarcity of probes targeting intramolecular associations between cell wall polymers especially hinders our understanding of the cell wall microstructure and affects the development of effective means for its efficient deconstruction for bioconversion. Here we report a detailed characterization of a cellulose-binding DNA aptamer CELAPT MINI using a combination of various in vitro biochemical, biophysical, and molecular biology techniques. Read More

View Article and Full-Text PDF

Introducing the Rapid Alert Supply Network Extractor (RASNEX) tool to mine supply chain information from food and feed contamination notifications in Europe.

PLoS One 2021 27;16(7):e0254301. Epub 2021 Jul 27.

German Federal Institute for Risk Assessment, Berlin, Germany.

Background: During food or feed contamination events, it is of utmost importance to ensure their rapid resolution to minimize impact on human health, animal health and finances. The existing Rapid Alert System for Food and Feed (RASFF) is used by the European Commission, national competent authorities of member countries and the European Food Safety Authority to report information on any direct or indirect human health risk arising from food or feed, or serious risks to animal health or the environment in relation to feed. Nevertheless, no methods exist to to collectively evaluate this vast source of supply chain information. Read More

View Article and Full-Text PDF

Amphipathic Barbiturates as Mimics of Antimicrobial Peptides and the Marine Natural Products Eusynstyelamides with Activity against Multi-resistant Clinical Isolates.

J Med Chem 2021 Jul 27. Epub 2021 Jul 27.

Department of Pharmacy, Faculty of Health Sciences, UiT The Arctic University of Norway, NO-9037 Tromsø, Norway.

We report a series of synthetic cationic amphipathic barbiturates inspired by the pharmacophore model of small antimicrobial peptides (AMPs) and the marine antimicrobials eusynstyelamides. These ,'-dialkylated-5,5-disubstituted barbiturates consist of an achiral barbiturate scaffold with two cationic groups and two lipophilic side chains. Minimum inhibitory concentrations of 2-8 μg/mL were achieved against 30 multi-resistant clinical isolates of Gram-positive and Gram-negative bacteria, including isolates with extended spectrum β-lactamase-carbapenemase production. Read More

View Article and Full-Text PDF

Polymer Network Editing of Elastomers for Robust Underwater Adhesion and Tough Bonding to Diverse Surfaces.

ACS Appl Mater Interfaces 2021 Jul 27. Epub 2021 Jul 27.

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3th Section, South Renmin Road, Wuhou District, Chengdu 610041, China.

Tough adhesives with robust adhesion are desperately needed for biomedical and technological applications. However, it is extremely challenging to engineer tough and durable adhesives that are simple to make yet also exhibit strong underwater adhesion as well as tough bonding to diverse surfaces. Here, we report bioinspired elastomers based on water-immiscible polydiolcitrates, where their tough mechanical properties, robust underwater adhesion (80 kPa), and tough bonding performance (with an interfacial toughness >1000 J m and a shear and tensile strength >0. Read More

View Article and Full-Text PDF

Ionotronic Tough Adhesives with Intrinsic Multifunctionality.

ACS Appl Mater Interfaces 2021 Jul 27. Epub 2021 Jul 27.

Department of Mechanical Engineering, McGill University, Montreal, Quebec H3A 0C3, Canada.

Ionotronic hydrogels find wide applications in flexible electronics, wearable/implantable devices, soft robotics, and human-machine interfaces. Their performance and practical translation have been bottlenecked by poor adhesiveness, limited mechanical properties, and the lack of biological functions. The remedies are often associated with complex formulations and sophisticated processing. Read More

View Article and Full-Text PDF

Three-dimensional label-free visualization and quantification of polyhydroxyalkanoates in individual bacterial cell in its native state.

Proc Natl Acad Sci U S A 2021 Aug;118(31)

Metabolic and Biomolecular Engineering National Research Laboratory, Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea;

Polyhydroxyalkanoates (PHAs) are biodegradable polyesters that are intracellularly accumulated as distinct insoluble granules by various microorganisms. PHAs have attracted much attention as sustainable substitutes for petroleum-based plastics. However, the formation of PHA granules and their characteristics, such as localization, volume, weight, and density of granules, in an individual live bacterial cell are not well understood. Read More

View Article and Full-Text PDF

Galectin-3 promotes noncanonical inflammasome activation through intracellular binding to lipopolysaccharide glycans.

Proc Natl Acad Sci U S A 2021 Jul;118(30)

Taiwan International Graduate Program in Molecular Medicine, National Yang-Ming University, Taipei 11221, Taiwan;

Cytosolic lipopolysaccharides (LPSs) bind directly to caspase-4/5/11 through their lipid A moiety, inducing inflammatory caspase oligomerization and activation, which is identified as the noncanonical inflammasome pathway. Galectins, β-galactoside-binding proteins, bind to various gram-negative bacterial LPS, which display β-galactoside-containing polysaccharide chains. Galectins are mainly present intracellularly, but their interactions with cytosolic microbial glycans have not been investigated. Read More

View Article and Full-Text PDF

Metal Cation Triggered Peptide Hydrogels and Their Application in Food Freshness Monitoring and Dye Adsorption.

Gels 2021 Jul 7;7(3). Epub 2021 Jul 7.

Dipartimento di Scienze Chimiche, Università degli Studi di Padova, via Marzolo 1, 35131 Padova, Italy.

Metal-ligand interactions have emerged as an important tool to trigger and modulate self-assembly, and to tune the properties of the final supramolecular materials. Herein, we report the metal-cation induced self-assembly of a pyrene-peptide conjugate to form hydrogels. The peptide has been rationally designed to favor the formation of β-sheet 1D assemblies and metal coordination through the Glu side chains. Read More

View Article and Full-Text PDF

Brain aggregoma with clonal B-cell perivascular proliferation detected by next-generation sequencing. A case report and review of the literature.

Folia Neuropathol 2021 ;59(2):205-211

Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

Light-chain deposition disease (LCDD), a rare type of monoclonal immunoglobulin deposition disease, can be presented as systemic or localized, very rarely affecting central nervous system (CNS). Only 10 cases of CNS-LCDD have been described so far. We present an eleventh case of cerebral tumour-like LCDD, called aggregoma, and compare it with previously reported cases. Read More

View Article and Full-Text PDF
January 2021

Structural Characterization of EnpA D,L-Endopeptidase from Prophage Provides Insights into Substrate Specificity of M23 Peptidases.

Int J Mol Sci 2021 Jul 1;22(13). Epub 2021 Jul 1.

International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland.

The best-characterized members of the M23 family are glycyl-glycine hydrolases, such as lysostaphin (Lss) from or LytM from . Recently, enzymes with broad specificities were reported, such as EnpA from , that cleaves D,L peptide bond between the stem peptide and a cross-bridge. Previously, the activity of EnpA was demonstrated only on isolated peptidoglycan fragments. Read More

View Article and Full-Text PDF

Additive Effects of Lithium Salts with Various Anionic Species in Poly (Methyl Methacrylate).

Molecules 2021 Jul 5;26(13). Epub 2021 Jul 5.

Polymer Physics Laboratory, Institute of Science and Engineering, Kakuma Campus, Kanazawa University, Kanazawa 920-1192, Ishikawa, Japan.

We report that lithium salts in lithium-ion batteries effectively modify the physical properties of poly (methyl methacrylate) (PMMA). The glass transition temperature () is an indicator of the heat resistance of amorphous polymers. The anionic species of the salts strongly affected the glass transition behavior of PMMA. Read More

View Article and Full-Text PDF

Induced permittivity increment of electrorheological fluids in an applied electric field in association with chain formation: A Brownian dynamics simulation study.

Phys Rev E 2021 Jun;103(6-1):062608

Research Centre for Engineering Sciences, University of Pannonia, Egyetem u. 10, Veszprém, 8200, Hungary and Institute of Mechatronics Engineering and Research, University of Pannonia, Gasparich Márk u. 18/A, Zalaegerszeg, 8900, Hungary.

We report Brownian dynamics simulation results for the relative permittivity of electrorheological (ER) fluids in an applied electric field. The relative permittivity of an ER fluid can be calculated from the Clausius-Mosotti (CM) equation in the small applied field limit. When a strong field is applied, however, the ER spheres are organized into chains and assemblies of chains in which case the ER spheres are polarized not only by the external field but by each other. Read More

View Article and Full-Text PDF

Antiresonant driven systems for particle manipulation.

Phys Rev E 2021 Jun;103(6-1):062102

Departament de Física de la Materia Condensada, Universitat de Barcelona, Av. Diagonal 647, 08028 Barcelona, Spain.

We report on the onset of antiresonant behavior of mass transport systems driven by time-dependent forces. Antiresonances arise from the coupling of a sufficiently high number of space-time modes of the force. The presence of forces having a wide space-time spectrum, a necessary condition for the formation of an antiresonance, is typical of confined systems with uneven and deformable walls that induce entropic forces dependent on space and time. Read More

View Article and Full-Text PDF

TEMPO Containing Radical Polymonothicarbonate Polymers with Regio- and Stereo-Regularities: Synthesis, Characterization, and Electrical Conductivity Studies.

Angew Chem Int Ed Engl 2021 Jul 16. Epub 2021 Jul 16.

Texas A&M University College Station: Texas A&M University, Chemistry, UNITED STATES.

Polymers bearing pendant radical functionalities have attracted much attention in recent years due to their wide spread applications, which span the areas of flexible magnetic, electronic and energy storage devices. Herein, we report the synthesis of a novel (2,2,6,6-tetramethylpiperidin-1-yl)oxidanyl) (TEMPO) appended polymonothiocarbonates through the ring-opening copolymerization of (4-glycidyloxy-2,2,6,6-tetramethylpiperidine-1-oxyl) (GTEMPO) in the presence of carbonyl sulfide under ambient conditions. We have prepared the atactic and isotactic versions of this polymer, using enantiopure R or S forms of the GTEMPO monomer in the latter instances. Read More

View Article and Full-Text PDF

Benzyltrimethylammonium cadmium dicyanamide with polar order in multiple phases and prospects for linear and nonlinear optical temperature sensing.

Dalton Trans 2021 Jul 16. Epub 2021 Jul 16.

Department of Experimental Physics, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.

Coordination polymers with multiple non-centrosymmetric phases have sparked substantial research efforts in the materials community. We report the synthesis and properties of a hitherto unknown cadmium dicyanamide coordination polymer comprising benzyltrimethylammonium cations (BeTriMe+). The room-temperature (RT) crystal structure of [BeTriMe][Cd(N(CN)2)3] (BeTriMeCd) is composed of Cd centers linked together by triple dca-bridges to form one-dimensional chains with BeTriMe+ cations located in void spaces between the chains. Read More

View Article and Full-Text PDF

Noncovalently functionalized commodity polymers as "tailor-made" additives for stereoselective crystallization.

Angew Chem Int Ed Engl 2021 Jul 15. Epub 2021 Jul 15.

Peking University, Chemistry College, Chengfu road 202, Haidian district, 100871, Haidian, CHINA.

Stereoselectively inhibiting the nucleation and crystal growth of one enantiomer aided by "tailor-made" polymeric additives is an efficient method to obtain enantiopure compounds. However, the conventional preparation of polymeric additives from chiral monomer is laborious and limited in structures, which impedes their rapid optimization and applicability. Herein, we report a "plug-and-play" strategy to facilitate synthesis by using commercially available achiral polymers as the platform to attach various chiral small molecules as the recognition side-chains through non-covalent interactions. Read More

View Article and Full-Text PDF

Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

medRxiv 2021 Jul 7. Epub 2021 Jul 7.

Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS , a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multi-parametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells. Read More

View Article and Full-Text PDF

Organocatalyzed Photoredox Radical Ring-Opening Polymerization of Functionalized Vinylcyclopropanes.

Macromolecules 2020 Oct 1;53(19):8352-8359. Epub 2020 Sep 1.

Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States.

Organocatalyzed photoredox radical ring-opening polymerization (rROP) of vinylcyclopropanes (VCPs) is employed for the synthesis of polymers with controlled molecular weight (MW), dispersity, and composition. Herein, we report the study on the rROP of a variety of VCP monomers bearing diverse functional groups (such as amide, alkene, ketal, urea, hemiaminal ether, and so on) under organocatalyzed conditions with varying light sources and temperature. Notably, VCP monomers bearing natural product functionality or their derivatives can be polymerized in a controlled manner to produce poly(VCPs) with predictable MW, low dispersity, tunable composition, high thermal stability, and tailored glass transition temperature ( ), ranging 39 to 107 °C. Read More

View Article and Full-Text PDF
October 2020

Vapor phase synthesis of ferroelectric microislands on PVDF thin films.

Nanotechnology 2021 Jul 15. Epub 2021 Jul 15.

Faculty of Engineering and Natural Sciences, Sabanci University, Orta Mahalle, Tuzla, Istanbul, 34956, TURKEY.

The interest in patterned PVDF surfaces has grown significantly in the recent years due to ability to control the ferroelectric behavior through the size and shape of the surface structures. However, forming micron sized structures on the PVDF surface generally requires laborious lithography based methods or use of templates which complicates the process. In this study, we report spontaneous formation of microislands with ferroelectric response during PVDF growth via initiated chemical vapor deposition. Read More

View Article and Full-Text PDF

Methemoglobin formation in mutant Hemoglobin α-chains: electron transfer parameters and rates.

Biophys J 2021 Jul 12. Epub 2021 Jul 12.

Department of Pharmacy, Birla Institute of Technology and Sciences Pilani (BITS-Pilani), Vidya Vihar Campus 41, Pilani, 333031, Rajasthan (India).

Hemoglobin mediated transport of dioxygen (O) critically depends on the stability of the reduced (Fe) form of the heme cofactors. Some protein mutations stabilize oxidized (Fe) state (Methemoglobin, Hb M) causing methemoglobinemia and can be lethal above 30 %. Majority of the analyses of factors influencing Hb oxidation are retrospective and give insights only for inner sphere mutations of heme (His58, His87). Read More

View Article and Full-Text PDF

A Crystalline BN Dewar Benzene as a Building Block for Conjugated B,N-Chains.

Authors:
Kei Ota Rei Kinjo

J Am Chem Soc 2021 Jul 15;143(29):11152-11159. Epub 2021 Jul 15.

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore.

Dewar benzene, one of the isolable valence isomers of CH, has been extensively studied since its first synthesis in 1962. By contrast, the chemistry of inorganic congeners of Dewar benzene, which can be formally gained by replacing the skeletal carbon atoms with heteroatoms, has been less developed despite their peculiar structural and electronic features. Among them, the extant B,N-Dewar benzenes are limited to the BN system. Read More

View Article and Full-Text PDF

First Report of Alternaria alternata Causing Leaf Spot on Chili (Capsicum annuum L.) in Pakistan.

Plant Dis 2021 Jul 14. Epub 2021 Jul 14.

Department of Agricultural Science, Okara, Punjab, Pakistan;

Chili (Capsicum annuum L.) is an important vegetable crop in Pakistan. During summer of 2019, chili leaf spot symptoms were observed on 3-month-old plants in the fields, with 30 to 40% of disease incidence, in District Faisalabad, Punjab, Pakistan. Read More

View Article and Full-Text PDF

Astral hydrogels mimic tissue mechanics by aster-aster interpenetration.

Nat Commun 2021 07 13;12(1):4277. Epub 2021 Jul 13.

School of Molecular Science and Engineering, South China University of Technology, Guangzhou, China.

Many soft tissues are compression-stiffening and extension-softening in response to axial strains, but common hydrogels are either inert (for ideal chains) or tissue-opposite (for semiflexible polymers). Herein, we report a class of astral hydrogels that are structurally distinct from tissues but mechanically tissue-like. Specifically, hierarchical self-assembly of amphiphilic gemini molecules produces radial asters with a common core and divergently growing, semiflexible ribbons; adjacent asters moderately interpenetrate each other via interlacement of their peripheral ribbons to form a gel network. Read More

View Article and Full-Text PDF