14,353 results match your criteria centrifugation time


OPTIMIZING A PLATELET-RICH PLASMA CONCENTRATION PROTOCOL FOR SOUTH AMERICAN SEA LIONS ().

J Zoo Wildl Med 2021 Sep;52(3):956-965

Veterinary Services, Oceanogràfic, Ciudad de las Artes y las Ciencias, C/Eduardo Primo Yúfera (Científic)1B, 46013 Valencia, Spain.

Accelerated healing in wild or captive South American sea lions () is a key tool to help minimize infection and complications associated with open wounds, dental disease, and ocular pathology. Platelet-rich plasma (PRP) is an autogenous source for growth factors based on platelet concentration, which can be obtained by centrifuging whole blood collected in sodium citrate anticoagulant. Currently, there are well-defined PRP concentration protocols for humans and most domestic companion animal species. Read More

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September 2021

Characterization of a New and Efficient Polyvalent Phage Infecting O157:H7, spp., and .

Microorganisms 2021 Oct 6;9(10). Epub 2021 Oct 6.

School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea.

Ongoing outbreaks of foodborne diseases remain a significant public health concern. Lytic phages provide promising attributes as biocontrol agents. This study characterized KFS-EC3, a polyvalent and lytic phage, which was isolated from slaughterhouse sewage and purified by cesium chloride density centrifugation. Read More

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October 2021

The Effect of a Single Freeze-Thaw Cycle on Matrix Metalloproteinases in Different Human Platelet-Rich Plasma Formulations.

Biomedicines 2021 Oct 6;9(10). Epub 2021 Oct 6.

Steadman Philippon Research Institute, Vail, CO 81657, USA.

Storing platelet-rich plasma (PRP) for future use is a compelling approach, presuming the retention of biological properties is maintained. However, certain factors in PRP preparations have deleterious effects for the treatment of certain musculoskeletal conditions. The purpose of this study was to measure and compare matrix metalloproteinase protein (MMP) concentrations between fresh and freeze-thawed leukocyte-rich PRP (LR-PRP) inactivated (LR-I) and activated (LR-A) preparations, and leukocyte-poor PRP (LP-PRP) inactivated (LP-I) and activated (LP-A) preparations. Read More

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October 2021

Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis.

Animals (Basel) 2021 Oct 9;11(10). Epub 2021 Oct 9.

Department de Medicina i Cirurgia Animal, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain.

The aim of this study was to standardize a simple, manual platelet-rich plasma (PRP) protocol in Catalonian donkeys using single-spin tube centrifugation as a treatment for jenny endometritis. The objective was to obtain a blood product with a moderate concentration of platelets (2 or 3 times baseline physiologic values) and a low WBC (White Blood Cells) concentration. Blood was drawn from six Catalonian donkeys using acid citrate dextrose (ACD) as an anticoagulant, and then processed by single centrifugation at 133× for two different centrifugation times (10 and 15 min). Read More

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October 2021

Acoustofluidic medium exchange for preparation of electrocompetent bacteria using channel wall trapping.

Lab Chip 2021 Oct 20. Epub 2021 Oct 20.

Mechanics and Experimental Dynamics, Department of Mechanical and Process Engineering, Swiss Federal Institute of Technology (ETH Zurich), Tannenstrasse 3, CH-8092 Zurich, Switzerland.

Comprehensive integration of process steps into a miniaturised version of synthetic biology workflows remains a crucial task in automating the design of biosystems. However, each of these process steps has specific demands with respect to the environmental conditions, including in particular the composition of the surrounding fluid, which makes integration cumbersome. As a case in point, transformation, reprogramming of bacteria by delivering exogenous genetic material (such as DNA) into the cytoplasm, is a key process in molecular engineering and modern biotechnology in general. Read More

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October 2021

Celecoxib decreases mitochondrial complex IV activity and induces oxidative stress in isolated rat heart mitochondria: An analysis for its cardiotoxic adverse effect.

J Biochem Mol Toxicol 2021 Oct 19:e22934. Epub 2021 Oct 19.

Traditional Medicine and Hydrotherapy Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.

In spite of the cardiotoxic effect of selective cyclooxygenase-2 inhibitors, they are most widely used as anti-inflammatory and analgesic drugs. Today, valdecoxib and rofecoxib have been withdrawn in the market but celecoxib remains. In this study, we focused on an analysis of celecoxib toxic effects on isolated mitochondria. Read More

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October 2021

Are isoforms of capacitating Na K -ATPase localized to sperm head rafts?

Mol Reprod Dev 2021 Oct 17. Epub 2021 Oct 17.

Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.

Capacitation begins in the sperm head plasma membrane (HPM). Membrane rafts could house signaling molecules, but although these specialized microdomains have been microscopically visualized in sperm heads, rafts have been isolated for study only from homogenized whole sperm or tails, never purified HPM. Sodium/potassium ATPase (Na K -ATPase) is a membrane-bound signaling protein that induces capacitation in bull sperm in response to the steroid hormone ouabain, and its subunit isoforms α1, α3, β1, β2, and β3 are known in HPM. Read More

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October 2021

promotes heterotopic ossification through upregulation of LncRNA MANCR.

Bone Joint Res 2021 Oct;10(10):668-676

Department of Spine Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Aims: Acquired heterotopic ossification (HO) is a debilitating disease characterized by abnormal extraskeletal bone formation within soft-tissues after injury. The exact pathogenesis of HO remains unknown. It was reported that may contribute to osteoblastic differentiation. Read More

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October 2021

A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts.

STAR Protoc 2021 Dec 30;2(4):100866. Epub 2021 Sep 30.

Baker Heart and Diabetes Institute, Melbourne, VIC 3004, Australia.

This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to perfuse the heart with enzymes to dissociate cells. Read More

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December 2021

Impact of centrifugation time and pneumatic tube transport on plasma concentrations of direct oral anticoagulants.

Int J Lab Hematol 2021 Oct 12. Epub 2021 Oct 12.

Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.

Introduction: Rapid results are needed when plasma concentrations of direct oral anticoagulants (DOACs) are required in acute clinical settings. We evaluated the impact of centrifugation time and pneumatic tube transport on DOAC plasma concentrations with the overall aim of reducing turnaround time.

Methods: Blood samples were spiked with rivaroxaban, apixaban or dabigatran in a low and a high concentration prior to centrifugation for 25 minutes (3163 g) or 5 minutes (3000 g) (n = 20 for each DOAC). Read More

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October 2021

Feasibility of using digital confocal microscopy for cytopathological examination in clinical practice.

Mod Pathol 2021 Oct 9. Epub 2021 Oct 9.

Department of Neurosurgery Research, The University of Texas, MD Anderson Cancer Center Houston, Houston, TX, USA.

Optical imaging modalities are emerging as digital microscopy tools for tissue examination. The investigation of these techniques for potential applications in anatomic pathology practice has focused primarily on surgical pathology and has not included cytopathological specimens. We evaluated the feasibility of using digital confocal microscopy (CM) to examine cytopathological specimens. Read More

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October 2021

SARS-CoV-2 RNA in urban wastewater samples to monitor the COVID-19 pandemic in Lombardy, Italy (March-June 2020).

Sci Total Environ 2021 Oct 8:150816. Epub 2021 Oct 8.

Department of Biomedical Sciences of Health, University of Milan, Via Pascal 36, 20133 Milan, Italy.

Wastewater-based viral surveillance was proposed as a promising approach to monitor the circulation of SARS-CoV-2 in the general population. The aim of this study was to develop an analytical method to detect SARS-CoV-2 RNA in urban wastewater, and apply it to follow the trends of epidemic in the framework of a surveillance network in the Lombardy region (Northern Italy). This area was the first hotspot of COVID-19 in Europe and was severely affected. Read More

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October 2021

Multiple heart-cutting two dimensional liquid chromatography and isotope dilution tandem mass spectrometry for the absolute quantification of proteins in human serum.

Anal Chim Acta 2021 Nov 3;1184:339022. Epub 2021 Sep 3.

Department of Physical and Analytical Chemistry, University of Oviedo, Calle Julián Clavería 8, 33006, Oviedo, Spain.

We evaluate here the combination of two-dimensional liquid chromatography (2D-LC) in the multiple heart cutting mode and isotope dilution tandem mass spectrometry for the direct analysis of tryptic digests of serum samples. As a proof of concept, we attempt the quantification of proteotypic peptides of Apolipoprotein AIV (APOA4), Complement C3 (C3) and Vitronectin (VTN) which have been previously identified as potential candidate biomarkers of glaucoma. Using this 2D-LC strategy, analyte enrichment steps are avoided and the sample preparation involved after enzymatic digestion amounted to a simple centrifugation, evaporation of the supernatant and reconstitution in the 1D mobile phase. Read More

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November 2021

Early and Rapid Detection of Typhoid Fever by Nested PCR in Blood.

Mymensingh Med J 2021 Oct;30(4):986-990

Dr Jobyda Khanam, Lecturer (Virology), Department of Microbiology, Mymensingh Medical College, (MMC), Mymensingh, Bangladesh; E-mail:

Typhoid fever caused by Salmonella typhi is one of the major health problems in developing countries including Bangladesh. Still now blood culture is gold standard method for diagnosing typhoid fever, but this method is laborious, requires several days and detection rate is low. Failure of early laboratory diagnosis often leads to increased morbidity and mortality. Read More

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October 2021

Prediction and evaluation of the effect of pre-centrifugation sample management on the measurable untargeted LC-MS plasma metabolome.

Anal Chim Acta 2021 Oct 19;1182:338968. Epub 2021 Aug 19.

Biobank West, Sahlgrenska University Hospital, Region Västra Götaland, Sweden; Institute of Biomedicine, Biobank Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Optimal handling is the most important means to ensure adequate sample quality. We aimed to investigate whether pre-centrifugation delay time and temperature could be accurately predicted and to what extent variability induced by pre-centrifugation management can be adjusted for. We used untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics to predict and evaluate the influence of pre-centrifugation temperature and delayed time on plasma samples. Read More

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October 2021

How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation.

J Biotechnol 2021 Sep 29;341:121-128. Epub 2021 Sep 29.

Department of Materials and Bioprocesses Engineering, School of Chemical Engineering, University of Campinas, P.O. Box 6066, 13083-852, Campinas, SP, Brazil.

Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas. Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components. Read More

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September 2021

Extracellular vesicles are the primary source of blood-borne tumour-derived mutant KRAS DNA early in pancreatic cancer.

J Extracell Vesicles 2021 Oct;10(12):e12142

Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.

Up to now, the field of liquid biopsies has focused on circulating tumour DNA and cells, though extracellular vesicles (EVs) have been of increasing interest in recent years. Thus, reported sources of tumour-derived nucleic acids include leukocytes, platelets and apoptotic bodies (AB), as well as large (LEV) and small (SEV) EVs. Despite these competing claims, there has yet to be a standardized comparison of the tumour-derived DNA associated with different components of blood. Read More

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October 2021

Alterations in Peripheral B Cell Subsets Correlate with the Disease Severity of Human Glaucoma.

J Inflamm Res 2021 21;14:4827-4838. Epub 2021 Sep 21.

Clinical Immunology Translational Medicine Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.

Background: Glaucoma is a group of retinal neurodegenerative diseases causing irreversible visual impairment. The pathogenesis of this disease is complicated. Studies have shown that the immune system is involved in the neurodegenerative process of glaucoma. Read More

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September 2021

Assessment of a Multiplex LAMP Assay (Eazyplex CSF Direct M) for Rapid Molecular Diagnosis of Bacterial Meningitis: Accuracy and Pitfalls.

Microorganisms 2021 Sep 1;9(9). Epub 2021 Sep 1.

Department of Microbiology, University Hospital of Nantes, 44093 Nantes, France.

Automated molecular panels are attractive tools for improving early meningitis diagnosis. This study assessed the Eazyplex CSF direct M panel (EP), a multiplex real-time Loop-Mediated Isothermal Amplification assay. From December 2016 to December 2019, cerebrospinal fluid (CSF) samples were routinely tested with the EP V1. Read More

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September 2021

An Integrated Approach for the Efficient Extraction and Solubilization of Rice Microsomal Membrane Proteins for High-Throughput Proteomics.

Front Plant Sci 2021 9;12:723369. Epub 2021 Sep 9.

Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, South Korea.

The preparation of microsomal membrane proteins (MPs) is critically important to microsomal proteomics. To date most research studies have utilized an ultracentrifugation-based approach for the isolation and solubilization of plant MPs. However, these approaches are labor-intensive, time-consuming, and unaffordable in certain cases. Read More

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September 2021

Comparison of two methods for isolation and culture of human foreskin fibroblasts.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Aug;46(8):800-808

Central Laboratory, Affiliated Haikou Hospital of Xiangya School of Medicine, Central South University, Haikou 570208.

Objectives: The efficient acquisition and purification of fibroblasts as ideal seed cells are very important. For optimization of the isolation and culture of human foreskin fibroblasts (HFF), we compared the improved tissue culture method (ITCM) and the enzyme digestion method (EDM).

Methods: In ITCM, the skin tissue was digested with 0. Read More

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Metabolite Ratios as Quality Indicators for Pre-Analytical Variation in Serum and EDTA Plasma.

Metabolites 2021 Sep 18;11(9). Epub 2021 Sep 18.

Institute of Clinical Chemistry and Laboratory Diagnostics and Integrated Biobank Jena (IBBJ), University Hospital Jena, Am Klinikum 1, 07747 Jena, Germany.

In clinical diagnostics and research, blood samples are one of the most frequently used materials. Nevertheless, exploring the chemical composition of human plasma and serum is challenging due to the highly dynamic influence of pre-analytical variation. A prominent example is the variability in pre-centrifugation delay (time-to-centrifugation; TTC). Read More

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September 2021

Effect of genus Clostridium abundance on mixed-culture fermentation converting food waste into biohydrogen.

Bioresour Technol 2021 Sep 20;342:125942. Epub 2021 Sep 20.

School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea. Electronic address:

This study examined the effect of various inocula on mixed-culture dark fermentative H production from food waste. Heat-treated and frozen H-producing granular sludge (HPG) grown with monomeric sugars showed a higher H yield, production rate, and acidogenic efficiency along with a shorter lag phase than heat-treated methanogenic sludge. Among three different methods of methanogenic sludge inoculation, inoculation after centrifugation showed better H production performance. Read More

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September 2021

Laboratory diagnosis of mange-causing mites in dogs using a modified centrifugation-flotation technique in sucrose solution.

Exp Appl Acarol 2021 Sep 24;85(1):41-48. Epub 2021 Sep 24.

Departamento de Medicina Veterinária do Instituto Federal de Educação, Ciência e Tecnologia da Paraíba (IFPB), Sousa, Paraíba, Brazil.

Among the skin disorders of dogs, scabies is notable for its high occurrence rate and the need for veterinary interventions. There are two obstacles to making this diagnosis through direct investigation under a microscope (DIM): the continual need to make new slides when the results are negative and the long time needed for reading these slides. Thus, the objective of the present study was to compare efficacy between DIM and the technique of centrifugation-flotation in sucrose solution (CFSS) in samples from dogs in the semiarid region of the state of Paraíba, Brazil. Read More

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September 2021

A standardized g-force allows the preparation of similar PRF qualities regardless of rotor angle.

Tissue Eng Part A 2021 Sep 24. Epub 2021 Sep 24.

Goethe-Universitat Frankfurt am Main, 9173, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University, Frankfurt Am Main, Germany, Frankfurt am Main, Hessen, Germany;

Platelet rich fibrin (PRF) is an autologous blood concentrate, that supports tissue regeneration. The effect of the centrifuge rotor angle in the fabrication of PRF is still not fully elucidated. The hypothesis of this study is: When applying the same g-force {relative centrifugal force (RCF)} and centrifugation time, PRF components and bioactivity are not modified using either a swing-out or fixed angle rotor. Read More

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September 2021

Matrix and Sampling Effects on Quantification of Protein Biomarkers of Drug-Induced Liver Injury.

J Proteome Res 2021 Sep 23. Epub 2021 Sep 23.

SIGNATOPE GmbH, Reutlingen 72770, Germany.

Macrophage colony stimulating factor 1 receptor (MCSF1R), osteopontin (OPN), high-mobility group protein B1 (HMGB1), glutamate dehydrogenase (GLDH), keratin 18 (K18), and caspase-cleaved keratin 18 (ccK18) are considered promising mechanistic biomarkers for the diagnosis of drug-induced liver injury. Here, we aim to elucidate the impact of the sample matrix and handling on the quantification of these emerging protein biomarkers. We investigated effects such as time from collection to centrifugation during serum (± gel) or EDTA plasma preparation on two assay platforms: immunoaffinity liquid chromatography mass spectrometric assays and sandwich immunoassays. Read More

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September 2021

adsorbent formation based dispersive micro-solid phase extraction using a deep eutectic solvent as an elution solvent for the extraction of some pesticides from honey samples prior to GC-MS analysis.

Anal Methods 2021 Oct 21;13(40):4724-4731. Epub 2021 Oct 21.

Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

In this work, a simple, inexpensive, green, and fast dispersive micro-solid phase extraction method has been developed for the extraction of several pesticides from honey samples. In this approach, a solution of curcumin was prepared in ethanol and it was dispersed into a sample solution with the aid of a syringe. Curcumin was precipitated in the sample solution as tiny particles and the analytes were adsorbed onto them. Read More

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October 2021

Host and geographic differences in prevalence and diversity of gastrointestinal helminths of foxes (), coyotes () and wolves () in Québec, Canada.

Int J Parasitol Parasites Wildl 2021 Dec 8;16:126-137. Epub 2021 Sep 8.

University of Saskatchewan, Department of Veterinary Microbiology, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada.

Wild canids are hosts to a wide range of parasites and can play a role in transmission of zoonoses. As many parasites are transmitted through food webs, and wild canids are at high trophic levels, parasite prevalence and diversity in wild canids can serve as excellent indicators of ecosystem health. Our main objectives were to update knowledge on the composition of gastrointestinal helminths in wild canids from Québec, Canada, and to describe differences in parasite prevalence and diversity among canid species and regions. Read More

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December 2021

Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient.

Methods Mol Biol 2022 ;2363:39-50

Department of Biology, Lund University, Lund, Sweden.

The isolation of mitochondria from potato tubers (Solanum tuberosum L.) is described, but the methodology can easily be adapted to other storage tissues. After homogenization of the tissue, filtration and differential centrifugation, the key step is a Percoll density gradient centrifugation. Read More

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January 2022

Difference in temporal and spatial distribution pattern of cyanobacteria between the sediment and water column in Lake Chaohu.

Environ Pollut 2021 Sep 11;291:118163. Epub 2021 Sep 11.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, China. Electronic address:

The evaluation about the relative distribution of cyanobacterial biomass between the sediment and water column would be indispensable to understand if benthic cyanobacteria are important to cyanobacterial biomass in the water column. A separation method for the rapid quantification of benthic cyanobacteria in Lake Chaohu was developed by density-gradient centrifugation. A 2 full factorial design and response surface methodology was employed to optimize the extraction protocol. Read More

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September 2021