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Phthalic acid esters degradation by a novel marine bacterial strain Mycolicibacterium phocaicum RL-HY01: Characterization, metabolic pathway and bioaugmentation.

Sci Total Environ 2021 Jun 5;791:148303. Epub 2021 Jun 5.

Shenzhen Research Institute of Guangdong Ocean University, Shenzhen 518108, China; College of Coastal Agricultural Sciences, School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China. Electronic address:

Phthalic acid esters (PAEs) are one of the most widely used plasticizers and the well-studied environmental pollutants with endocrine disrupting properties. Investigation about PAEs in terrestrial ecosystem has been extensively conducted while the fate of PAEs in marine environment remains underexplored. In this study, a novel di-(2-ethylhexyl) phthalate (DEHP) degrading marine bacterial strain, Mycolicibacterium phocaicum RL-HY01, was isolated and characterized from intertidal sediments. Read More

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In vitro generation of oxidized standards for lipidomics. Application to major membrane lipid components.

J Chromatogr A 2021 May 19;1651:462254. Epub 2021 May 19.

Centre for Metabolomics and Bioanalysis (CEMBIO), Department of Chemistry and Biochemistry, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Madrid. Spain. Electronic address:

Membrane lipids (sphingolipids, glycerophospholipids, cardiolipins, and cholesteryl esters) are critical in cellular functions. Alterations in the levels of oxidized counterparts of some of these lipids have been linked to the onset and development of many pathologies. Unfortunately, the scarce commercial availability of chemically defined oxidized lipids is a limitation for accurate quantitative analysis, characterization of oxidized composition, or testing their biological effects in lipidomic studies. Read More

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Peripheral intravenous catheter management in childbirth (PICMIC): A multi-centre, prospective cohort study.

J Adv Nurs 2021 Jun 12. Epub 2021 Jun 12.

School of Nursing, Midwifery and Social Work, University of Queensland, Brisbane, QLD, Australia.

Background: Childbirth is a normal, physiological process, yet intervention is common. Arguably the most common intervention is the insertion of a peripheral intravenous catheter; however, there are few studies guiding best practice. This study aimed to describe current intravenous catheter insertion practice, explore clinician decision-making during insertion and perceptions of women. Read More

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Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography -Tandem Mass Spectrometry.

Methods Mol Biol 2021 ;2275:329-339

Laboratory division HUSLAB, Helsinki University Central Hospital, Helsinki, Finland.

Coenzyme Q10 (CoQ) is an essential part of the mitochondrial respiratory chain . Here, we describe an accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of mitochondrial CoQ in isolated mitochondria . In the assay, mitochondrial suspensions are spiked with CoQ-[H] internal standard (IS), extracted with organic solvents and CoQ quantified by LC-MS/MS using multiple reaction monitoring (MRM). Read More

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January 2021

Outwelling of total alkalinity and dissolved inorganic carbon from the Hooghly River to the adjacent coastal Bay of Bengal.

Environ Monit Assess 2021 Jun 12;193(7):415. Epub 2021 Jun 12.

School of Marine Science, University of Maine, Orono, ME, USA.

The seasonal variability of the lateral flux of total alkalinity (TAlk) and dissolved inorganic carbon (DIC) of the tropical Hooghly estuary is analyzed in this work. In situ observations of water temperature, salinity, dissolved oxygen, TAlk, and pH were measured in four different stations of the Hooghly estuary. It was measured once every month during 2015-2016, and subsequently, DIC was estimated. Read More

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Optimising breast cancer screening reading: blinding the second reader to the first reader's decisions.

Eur Radiol 2021 Jun 12. Epub 2021 Jun 12.

Department of Health Sciences, Warwick Medical School, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK.

Objectives: In breast cancer screening, two readers separately examine each woman's mammograms for signs of cancer. We examined whether preventing the two readers from seeing each other's decisions (blinding) affects behaviour and outcomes.

Methods: This cohort study used data from the CO-OPS breast-screening trial (1,119,191 women from 43 screening centres in England) where all discrepant readings were arbitrated. Read More

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The associations between bitter and fat taste sensitivity, and dietary fat intake: Are they impacted by genetic predisposition?

Chem Senses 2021 Jun 12. Epub 2021 Jun 12.

Faculty of Sport, Allied Health and Performance Sciences, Department of Health Science, St Mary's University Twickenham, UK.

A relationship between bitter and fat taste sensitivity, CD36 rs1761667 and TAS2R38 has been demonstrated. However, research is scarce and does not take diet into account. This study aimed to explore associations between genetics, fat and bitter taste sensitivity and dietary fat intake in healthy UK adults. Read More

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Nicotinic acid supplementation at a supraphysiological dose increases the bioavailability of NAD precursors in mares.

J Anim Physiol Anim Nutr (Berl) 2021 Jun 12. Epub 2021 Jun 12.

Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia.

NAD deficiency has recently been linked with increased occurrences of congenital abnormalities and embryonic death in human and animal subjects. Early embryonic death is a major component of pregnancy loss in mares and very little is known regarding the requirement for NAD in horses. The aim of this study was to quantify NAD and its metabolites in the plasma and urine of mares after orally administering an acute dose of nicotinic acid and determine the absorption, metabolism and excretion of this essential precursor for NAD biosynthesis. Read More

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Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds.

Nat Protoc 2021 Jun 11. Epub 2021 Jun 11.

Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia.

The L-tryptophan-derived tricyclic hydroperoxide cis-WOOH was recently identified as a novel and biologically important factor for regulating vascular tone and blood pressure under inflammatory conditions and potentially other cellular redox signaling events. cis-WOOH is highly labile and currently not available commercially. In this protocol, we provide procedures for the synthesis, purification, quantification and characterization of cis-WOOH, its epimer trans-WOOH and their respective alcohols (cis-WOH and trans-WOH). Read More

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Reliable identification of protein-protein interactions by crosslinking mass spectrometry.

Nat Commun 2021 Jun 11;12(1):3564. Epub 2021 Jun 11.

Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany.

Protein-protein interactions govern most cellular pathways and processes, and multiple technologies have emerged to systematically map them. Assessing the error of interaction networks has been a challenge. Crosslinking mass spectrometry is currently widening its scope from structural analyses of purified multi-protein complexes towards systems-wide analyses of protein-protein interactions (PPIs). Read More

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Chronic Disease, Allergies, and Increased Years of Running Are Risk Factors Predicting Gradual Onset Running-Related Injuries in Ultramarathon Runners-SAFER XIX Study in 29 585 Race Entrants.

Clin J Sport Med 2021 Jun 9. Epub 2021 Jun 9.

Sport Exercise Medicine and Lifestyle Institute (SEMLI) and Section Sports Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa; Sport Exercise Medicine and Lifestyle Institute (SEMLI), Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa; Emeritus Professor of Sport and Exercise Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa; International Olympic Committee (IOC) Research Centre, Cape Town, South Africa; Biostatistics Unit, Medical Research Council, Cape Town, South Africa; Department of Human Movement Science, CPUT, Cape Town, South Africa; and Statistics and Population Studies Department, University of the Western Cape, Cape Town, South Africa.

Objectives: To identify risk factors that predict gradual onset running-related injuries (GORRIs) in ultramarathon runners entering a mass community-based event.

Design: Descriptive cross-sectional study.

Setting: Two Oceans 56 km ultramarathon 2012 to 2015. Read More

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Metabolic adaptation is associated with less weight and fat mass loss in response to low-energy diets.

Nutr Metab (Lond) 2021 Jun 11;18(1):60. Epub 2021 Jun 11.

Department of Nutrition Sciences, University of Alabama At Birmingham, Birmingham, USA.

Background: The practical relevance of metabolic adaptation remains a controversial issue. To the best of our knowledge, no study has properly evaluated the role of metabolic adaptation in modulating weight loss outcomes. Therefore, the aim of this study was to determine the association between metabolic adaptation, at the level of resting metabolic rate (RMR), and weight and fat mass (FM) loss after low-energy diets (LED), after adjusting for dietary adherence and other confounders. Read More

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Environmental risks related to organic compounds from the combustion of paper briquettes in domestic boilers.

J Hazard Mater 2021 Jun 2;418:126291. Epub 2021 Jun 2.

Centre ENET - Energy Units for Utilization of Non-Traditional Energy Sources, VŠB - Technical University of Ostrava, 17. listopadu 15/2172, 708 00 Ostrava-Poruba, Czech Republic. Electronic address:

Environmental risks connected with the combustion of paper/cardboard briquettes are still not sufficiently known. This paper aims to bring attention to the risks related to the utilisation of paper briquettes in local boilers and to characterise these risks by means of the identification of organic compounds in deposits from exhaust flues. The identification of the chemical compounds was performed by pyrolysis gas chromatography with mass spectrometric detection. Read More

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Adiabatic dynamic causal modelling.

Neuroimage 2021 Jun 8:118243. Epub 2021 Jun 8.

The Wellcome Centre for Human Neuroimaging, UCL Queen Square Institute of Neurology, United Kingdom.

This technical note introduces adiabatic dynamic causal modelling, a method for inferring slow changes in biophysical parameters that control fluctuations of fast neuronal states. The application domain we have in mind is inferring slow changes in variables (e.g. Read More

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Cytomegalovirus subverts macrophage identity.

Cell 2021 Jun 5. Epub 2021 Jun 5.

Institute for Immunodeficiency, Center for Chronic Immunodeficiency (CCI), University Medical Center, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany; Center for Pediatrics and Adolescent Medicine, University Medical Center, 79106 Freiburg, Germany. Electronic address:

Cytomegaloviruses (CMVs) have co-evolved with their mammalian hosts for millions of years, leading to remarkable host specificity and high infection prevalence. Macrophages, which already populate barrier tissues in the embryo, are the predominant immune cells at potential CMV entry sites. Here we show that, upon CMV infection, macrophages undergo a morphological, immunophenotypic, and metabolic transformation process with features of stemness, altered migration, enhanced invasiveness, and provision of the cell cycle machinery for viral proliferation. Read More

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Oxylipin profiles and levels vary by skeletal muscle type, dietary fat and sex in young rats.

Appl Physiol Nutr Metab 2021 Jun 11. Epub 2021 Jun 11.

University of Manitoba Faculty of Agricultural and Food Sciences, 98691, Human Nutritional Sciences, Winnipeg, Manitoba, Canada.

PUFA-derived bioactive lipid mediators called oxylipins have been shown to influence muscle growth, inflammation and repair in select muscles. Since individual oxylipins have varying effects and potencies, broad profiling in differing muscle types is required to further understand their overall effects. In addition, diet and sex are key determinants of oxylipin levels. Read More

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CSNK2A1-mediated phosphorylation of HMGA2 modulates cisplatin resistance in cervical cancer.

FEBS Open Bio 2021 Jun 11. Epub 2021 Jun 11.

Department of Urology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221002, China.

The development of chemoresistance reduces the efficacy of anti-cancer drugs. Cervical cancer is still one of the most common cancer types in developing countries. The oncogene HMGA2 is involved in the development and progression of tumors, but its role in chemoresistance of cervical cancer remain unclear. Read More

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Should nutritional therapy be modified to account for mitochondrial dysfunction in critical illness?

JPEN J Parenter Enteral Nutr 2021 Jun 11. Epub 2021 Jun 11.

William Harvey Research Institute, Queen Mary University of London, London.

Metabolic dysfunction, and its associated muscle atrophy, remains the most common complication of critical care. At the centre of this is mitochondrial dysfunction, secondary to hypoxia and systemic inflammation. This leads to a bioenergetic crisis, with decreased intramuscular adenosine tri-phosphate content and a reduction in the highly energy dependent process of protein synthesis. Read More

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Genetic sensitivity analysis: Adjusting for genetic confounding in epidemiological associations.

PLoS Genet 2021 Jun 11;17(6):e1009590. Epub 2021 Jun 11.

Department of Health Sciences, University of Leicester, Leicester, United Kingdom.

Associations between exposures and outcomes reported in epidemiological studies are typically unadjusted for genetic confounding. We propose a two-stage approach for estimating the degree to which such observed associations can be explained by genetic confounding. First, we assess attenuation of exposure effects in regressions controlling for increasingly powerful polygenic scores. Read More

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Ayurveda botanicals in COVID-19 management: An in silico multi-target approach.

PLoS One 2021 11;16(6):e0248479. Epub 2021 Jun 11.

AYUSH-Center of Excellence, Center for Complementary and Integrative Health, Interdisciplinary School of Health Sciences, Savitribai Phule Pune University, Pune, India.

The Coronavirus disease (COVID-19) caused by the virus SARS-CoV-2 has become a global pandemic in a very short time span. Currently, there is no specific treatment or vaccine to counter this highly contagious disease. There is an urgent need to find a specific cure for the disease and global efforts are directed at developing SARS-CoV-2 specific antivirals and immunomodulators. Read More

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Effects of additional load at different heights on gait initiation: A statistical parametric mapping of center of pressure and center of mass behavior.

PLoS One 2021 11;16(6):e0242892. Epub 2021 Jun 11.

Physical Education Department, Federal University of São Carlos, São Carlos, Brazil.

The purpose of this study was to investigate the effects of different vertical positions of an asymmetrical load on the anticipatory postural adjustments phase of gait initiation. Sixty-eight college students (32 males, 36 females; age: 23.65 ± 3. Read More

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High risk of early sub-therapeutic penicillin concentrations after intramuscular benzathine penicillin G injections in Ethiopian children and adults with rheumatic heart disease.

PLoS Negl Trop Dis 2021 Jun 11;15(6):e0009399. Epub 2021 Jun 11.

Faculty of Health and Medical Sciences, University of Western Australia, Perth, Western Australia, Australia.

Introduction: Intramuscular benzathine penicillin G (BPG) injections are a cornerstone of secondary prophylaxis to prevent acute rheumatic fever (ARF) and rheumatic heart disease (RHD). Uncertainties regarding inter-ethnic and preparation variability, and target exposure profiles of BPG injection are key knowledge gaps for RHD control.

Methods: To evaluate BPG pharmacokinetics (PK) in patients receiving 4-weekly doses in Ethiopia, we conducted a prospective cohort study of ARF/RHD patients attending cardiology outpatient clinics. Read More

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A Case Report of Subclinical Myasthenia Gravis Associated with Castleman's Disease.

Am J Case Rep 2021 Jun 11;22:e930948. Epub 2021 Jun 11.

Department of Thoracic Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

BACKGROUND Castleman's disease is defined as a benign lymphoproliferative disorder of uncertain origin. It is most commonly found in the area of mediastinum. Castleman's disease is classified based on pathological features into hyaline-vascular, plasma cell, and mixed variants, which the hyaline-vascular variant is the commonest in association with Myasthenia Gravis. Read More

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Associations between efavirenz concentrations, pharmacogenetics, and neurocognitive performance in people living with HIV in Nigeria.

AIDS 2021 Jun 9. Epub 2021 Jun 9.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ibadan, Nigeria Centre for Drug Discovery, Development and Production (CDDDP), Faculty of Pharmacy, University of Ibadan Division of HIV, Infectious Diseases, and Global Medicine, University of California, San Francisco, USA Department of Pharmacology and Therapeutics, University of Liverpool, UK Division of Infectious Diseases and Center for Global Health, Northwestern University, Chicago, USA Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria Department of Neurology, University of North Carolina, Chapel Hill, NC, USA.

Objective: Efavirenz use is associated with neuropsychiatric side effects, which may include poor neurocognitive performance. We evaluated single nucleotide polymorphisms (SNPs) in genes that contribute to efavirenz pharmacokinetics and examined them in association with EFV concentrations in plasma and hair, as well as neurocognitive performance.

Design: Cross-sectional study where adults with HIV (PWH) receiving 600 mg efavirenz for at least two months were recruited and paired hair and dried blood spots (DBS) samples collected. Read More

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MS2Compound: A User-Friendly Compound Identification Tool for LC-MS/MS-Based Metabolomics Data.

OMICS 2021 Jun;25(6):389-399

Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, India.

Metabolomics is a leading frontier of systems science and biomedical innovation. However, metabolite identification in mass spectrometry (MS)-based global metabolomics investigations remains a formidable challenge. Moreover, lack of comprehensive spectral databases hinders accurate identification of compounds in global MS-based metabolomics. Read More

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Testicular torsion-detorsion causes dysfunction of mitochondrial oxidative phosphorylation.

Andrology 2021 Jun 11. Epub 2021 Jun 11.

Integrative Research Centre for Critical Care, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan.

Background: Semen quality impairment is a serious consequence of testicular torsion-detorsion (TD). Adequate germ-cell mitochondrial oxidative phosphorylation (OXPHOS) plays a crucial role in male fertility. Changes in cellular OXPHOS in testicular tissues after testicular TD remain unclear. Read More

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The Number of Pills, Rather Than the Type of Renin-Angiotensin System Inhibitor, Predicts Ambulatory Blood Pressure Control in Essential Hypertensives on Triple Therapy: A Real-Life Cross-Sectional Study.

Adv Ther 2021 Jun 11. Epub 2021 Jun 11.

Internal Medicine and Geriatrics, "Hypertension Excellence Centre" of the European Society of Hypertension, IRCCS INRCA, Ancona, Italy.

Introduction: We evaluated the prevalence and predictors of ambulatory blood pressure (BP) control in patients taking a triple antihypertensive therapy (renin-angiotensin system inhibitor + calcium channel blocker + thiazide/thiazide-like diuretic, in either free or fixed-dose combinations) containing an angiotensin-converting enzyme inhibitor (ACEi) or an angiotensin receptor blocker (ARB).

Methods: We performed an observational cross-sectional study on 520 consecutive patients with essential hypertension taking a stable triple therapy in whom 24-h ambulatory BP was evaluated. Both number of pills and antihypertensive treatment intensity (ATI), as possible pharmacological predictors of ambulatory BP control, were taken into account. Read More

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Consistent scaling of whole-shoot respiration between Moso bamboo (Phyllostachys pubescens) and trees.

J Plant Res 2021 Jun 11. Epub 2021 Jun 11.

Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.

Both Moso bamboo (Phyllostachys pubescens) and tree forests have a large biomass; they are considered to play an important role in ecosystem carbon budgets. The scaling relationship between individual whole-shoot (i.e. Read More

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Identification and Evaluation of Serum Protein Biomarkers Which Differentiate Psoriatic from Rheumatoid Arthritis.

Arthritis Rheumatol 2021 Jun 11. Epub 2021 Jun 11.

UCD Conway Institute of Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Ireland.

Objectives: To identify serum protein biomarkers which might separate early inflammatory arthritis (EIA) patients with psoriatic arthritis (PsA) from those with rheumatoid arthritis (RA) and may be used to support appropriate early intervention.

Methods: The serum proteome of patients with PsA and RA was interrogated using nano-flow liquid chromatography mass spectrometry (nLC-MS/MS) (n=64 patients), an aptamer-based assay (SOMAscan) targeting 1,129 proteins (n=36 patients) and a multiplexed antibody assay (Luminex) for 48 proteins (n=64 patients). Multiple reaction monitoring assays (MRM) were developed to evaluate the performance of putative markers using the discovery cohort (n=60) and subsequently an independent cohort of PsA and RA patients (n=167). Read More

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Spatial relationship between the pulmonary trunk and the left coronaries: Systematic risk assessment based on automated three-dimensional distance measurements.

Heart Rhythm O2 2020 Apr 27;1(1):14-20. Epub 2020 Apr 27.

Informatics for Life, Heidelberg, Germany.

Background: Catheter ablation of right ventricular outflow tract ventricular arrhythmias from above the pulmonary valve is being increasingly reported.

Objective: The purpose of this study was to systematically analyze the spatial relationship between the pulmonary trunk and the left coronaries.

Methods: Contrast-enhanced computed tomographic scans from 58 patients were analyzed. Read More

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