6,085,035 results match your criteria cellular


Evidence for Role of Capillaries in Regulation of Skeletal Muscle Oxygen Supply.

Microcirculation 2021 Apr 14:e12699. Epub 2021 Apr 14.

Biomedical Engineering Graduate Program, The University of Western Ontario, London, Ontario, Canada.

How oxygen (O ) supply to capillaries is regulated to match the tissue's demand is unknown. Erythrocytes have been proposed as sensors in this regulatory mechanism since they release ATP, a vasodilator, in an oxygen saturation (SO ) dependent manner. ATP causes hyperpolarization of endothelial cells resulting in conducted vasodilation in arterioles. Read More

View Article and Full-Text PDF

Mechanisms and functions of membrane lipid remodeling in plants.

Plant J 2021 Apr 14. Epub 2021 Apr 14.

Biology Department, Brookhaven National Laboratory, Upton, NY, 11973, USA.

Lipid remodeling, defined herein as post-synthetic structural modifications of membrane lipids, play crucial roles in regulating the physicochemical properties of cellular membranes and hence their many functions. Processes affected by lipid remodeling include lipid metabolism, membrane repair, cellular homeostasis, fatty acid trafficking, cellular signaling and stress tolerance. Glycerolipids are the major structural components of cellular membranes and their composition can be adjusted by modifying their head groups, their acyl chain lengths and the number and position of double bonds. Read More

View Article and Full-Text PDF

Minimal Residual Disease in Neovascular Age-Related Macular Degeneration.

Klin Monbl Augenheilkd 2021 Apr 14. Epub 2021 Apr 14.

Augenklinik der LMU, Klinikum der Universität München, München, Germany.

With the advent of long-acting anti-vascular endothelial growth factor substances, "healing of AMD (age-related macular degeneration)" might be easily assumed due to prolonged treatment intervals. Adoption of the oncological concept of minimal residual disease for quiescent choroidal neovascularization (CNV) lesions might enhance the importance of continued monitoring, and spur research into the core of the disease, i.e. Read More

View Article and Full-Text PDF

[Anti-Aging - healthy aging].

Authors:
Thomas Münzer

Dtsch Med Wochenschr 2021 Apr 14;146(8):543-551. Epub 2021 Apr 14.

Anti-aging products still are a big business worldwide. The most active promoters of anti-aging products currently are internet dealers, the cosmetic and the dairy industry or companies that sell nutritional supplements. Only a few of the advertised substances however have been studied in clinical trials under robust conditions. Read More

View Article and Full-Text PDF

[CpG-ODN instillation boosts ICAM-1 expression in an orthotopic murine UCC model: immunohistochemical evaluation of the local response to immunostimulatory DNA].

Aktuelle Urol 2021 Apr 14. Epub 2021 Apr 14.

Urologisches Zentrum Mittelhessen, DRK-Krankenhaus Biedenkopf und Universitätsklinikum Gießen und Marburg, Klinik für Strahlentherapie, Standort Marburg.

Background: Immunostimulatory CpG oligodeoxynucleotides (CpG-ODN) have been verified as an effective antineoplastic agent for intravesical application in a murine orthotopic C57-BL6 /MB-49 urothelial cell carcinoma (UCC). To date, many details in the mode of action have remained unclear. Preceding studies pointed towards a Th1-weighted response. Read More

View Article and Full-Text PDF

Preventing Brain Damage from Hypoxic-Ischemic Encephalopathy in Neonates: Update on Mesenchymal Stromal Cells and Umbilical Cord Blood Cells.

Am J Perinatol 2021 Apr 14. Epub 2021 Apr 14.

Department of Pediatrics, Faculty of Medicine, Osaka City University, Osaka, Japan.

Objective:  Neonatal hypoxic-ischemic encephalopathy (HIE) causes permanent motor deficit "cerebral palsy (CP)," and may result in significant disability and death. Therapeutic hypothermia (TH) had been established as the first effective therapy for neonates with HIE; however, TH must be initiated within the first 6 hours after birth, and the number needed to treat is from 9 to 11 to prevent brain damage from HIE. Therefore, additional therapies for HIE are highly needed. Read More

View Article and Full-Text PDF

A Withanolide-rich Fraction of Athenaea velutina Induces Apoptosis and Cell Cycle Arrest in Melanoma B16F10 Cells.

Planta Med 2021 Apr 14. Epub 2021 Apr 14.

Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Brazil.

is a promising Brazilian shrub with cytotoxic and antimigratory properties against cancer cells. However, the mechanism of induction of cancer cell death and the compounds involved remain unknown. To ascertain these bioactive compounds, bioassay-guided fractionation was performed, alongside the appropriate tests. Read More

View Article and Full-Text PDF

An Autopsy Case of Spontaneous Coronary Artery Dissection With Eosinophilic Coronary Periarteritis and Degeneration of Medial Smooth Muscles.

Am J Forensic Med Pathol 2021 Apr 13. Epub 2021 Apr 13.

From the Department of Forensic Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya Department of Anatomic Pathology, Hirosaki University Graduate School of Medicine, Hirosaki Department of Legal Medicine, University of Yamanashi Graduate School of Medicine, Chuo, Japan.

Abstract: A 45-year-old woman with no known medical history died suddenly shortly after complaining of anterior chest discomfort. The autopsy revealed a dissection at the anterior descending branch of the left coronary artery, and eosinophilic adventitial inflammation was observed both in the right coronary artery and in the vicinity of the dissection. Furthermore, there was degeneration of the tunica media in the right coronary artery, and this was thought to be a predissection lesion. Read More

View Article and Full-Text PDF

Exosomal circ_0004136 enhances the progression of pediatric acute myeloid leukemia depending on the regulation of miR-570-3p/TSPAN3 axis.

Anticancer Drugs 2021 Apr 12. Epub 2021 Apr 12.

Department of Pediatrics, Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei, China.

Circular RNAs (circRNAs) have been implicated in the progression of pediatric acute myeloid leukemia (AML). Although circ_0004136 has been found to play a crucial role in AML, our understanding of its molecular mechanism remains very limited. The levels of circ_0004136, miR-570-3p and tetraspanin 3 (TSPAN3) were determined by quantitative real-time PCR or western blot. Read More

View Article and Full-Text PDF

MAP17 Expression in Colorectal Cancer Is a Prognostic Factor for Disease Recurrence and Dismal Prognosis Already in Early Stage Disease.

Oncology 2021 Apr 14:1-11. Epub 2021 Apr 14.

2nd Department of Propedeutic Surgery, Athens University Medical School, Laiko General Hospital, Athens, Greece.

Background: Disease recurrence in colorectal cancer constitutes a major cause of significant cancer-associated morbidity and mortality. MAP17 is a small protein, and its overexpression in malignant tumors has been correlated with aggressive tumor phenotypes. The aim of the present study was to investigate the expression patterns of MAP17 in colorectal cancer specimens and to assess its clinical significance. Read More

View Article and Full-Text PDF

Transamniotic Stem Cell Therapy for Experimental Congenital Diaphragmatic Hernia: Structural, Transcriptional, and Cell Kinetics Analyses in the Nitrofen Model.

Fetal Diagn Ther 2021 Apr 14:1-11. Epub 2021 Apr 14.

Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Purpose: We examined select pulmonary effects and donor cell kinetics after transamniotic stem cell therapy (TRASCET) in a model of congenital diaphragmatic hernia (CDH).

Methods: Pregnant dams (n = 58) received nitrofen on gestational day 9.5 (E9) to induce fetal CDH. Read More

View Article and Full-Text PDF

Immune Response of Gamma-Irradiated Inactivated Bivalent Polio Vaccine Prepared plus Trehalose as a Protein Stabilizer in a Mouse Model.

Intervirology 2021 Apr 14:1-7. Epub 2021 Apr 14.

Faculty of Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran.

Introduction: Poliovirus causes paralysis by infecting the nervous system. Currently, 2 types of polio vaccine are given in many countries in polio eradication program including inactivated polio vaccine (IPV) and oral polio vaccine (OPV). Because of OPV-related paralysis, OPV should be replaced by IPV. Read More

View Article and Full-Text PDF

A note on the power per mitochondrion and the number of associated active ATP synthases.

Phys Biol 2021 Apr 14. Epub 2021 Apr 14.

Department of Chemistry and Physics, Mount Saint Vincent University, Halifax, Nova Scotia, B3M 2J6, Halifax, Nova Scotia, B3M 2J6, CANADA.

Recent experiments and thermodynamic arguments suggest that mitochondrial temperatures are higher than those of the cytoplasm. A "hot mitochondrion" calls for a closer examination of the energy balance that endows it with these claimed elevated temperatures. As a first step in this effort, we present here a semi-quantitative bookkeeping whereby, in one stroke, a formula is proposed that yields the rate of heat production in a typical mitochondrion and a formula for estimating the number of active ATP synthase molecules per mitochondrion. Read More

View Article and Full-Text PDF

Mathematical modeling of chemotaxis guided amoeboid cell swimming.

Authors:
Wang Qixuan Wu Hao

Phys Biol 2021 Apr 14. Epub 2021 Apr 14.

Polymer Science and Engineering, University of Massachusetts Amherst, Conte A325, 120 Governors Drive, Amherst, Massachusetts, 01003, UNITED STATES.

Cells and microorganisms adopt various strategies to migrate in response to different environmental stimuli. To date, many modeling research has focused on the crawling-based Dictyostelium discoideum (Dd) cells migration induced by chemotaixs, yet recent experimental results reveal that even without adhesion or contact to a substrate, Dd cells can still swim to follow chemoattractant signals. In this paper, we develop a modeling framework to investigate the chemotaxis induced amoeboid cell swimming dynamics. Read More

View Article and Full-Text PDF

Using a Safe and Effective Fixative to Improve the Immunofluorescence Staining of Bacteria.

Methods Appl Fluoresc 2021 Apr 14. Epub 2021 Apr 14.

Guangxi Medical University, 22 Shuangyong Road, Nanning, Guangxi, China, Nanning, 530021, CHINA.

The emerging and development of green chemistry has once again drawn the researchers' attention to eliminating the use and generation of hazardous materials. Here we report the use of a safe and effective fixative, chlorine dioxide (ClO2), instead of traditional hazardous fixatives for the cross-linking of cellular proteins to improve immunofluorescence staining of bacteria. The concentration of ClO2 needed for 100 % fixation is 50 g/mL, which is much lower than that of traditional fixatives (1000-10000 g/mL). Read More

View Article and Full-Text PDF

Bioprinted nanocomposite hydrogels: A proposed approach to functional restoration of skeletal muscle and vascular tissue following volumetric muscle loss.

Curr Opin Pharmacol 2021 Apr 11;58:35-43. Epub 2021 Apr 11.

Bone Muscle Research Center, College of Nursing & Health Innovation, The University of Texas at Arlington, 701 South Nedderman Drive, Arlington, TX, 76019, USA; Department of Nursing, College of Nursing & Health Innovation, The University of Texas at Arlington, 411 South Nedderman Drive Box 19407, Arlington, TX, 76019, USA. Electronic address:

Musculoskeletal conditions are the highest contributor to global disability, accounting for 16% of all ages lived with disability. Volumetric muscle loss (VML) is classified as significant damage to skeletal muscle compartments and motor units, leading to significant tissue loss, functional deficits, and long-term disability. In this review, the current tissue engineering approaches in terms of fabrication techniques, materials, cell sources, and growth factors for enhanced angiogenesis and neuromuscular junction (NMJ) in VML repair, are discussed. Read More

View Article and Full-Text PDF

Energy metabolism disorder mediated ammonia gas-induced autophagy via AMPK/mTOR/ULK1-Beclin1 pathway in chicken livers.

Ecotoxicol Environ Saf 2021 Apr 11;217:112219. Epub 2021 Apr 11.

College of Animal Science and Technology, Northeast Agricultural University, NO. 600 Chang Jiang Road, Xiang Fang District, Harbin 150030, China. Electronic address:

Ammonia gas is a well-known environmental pollution gas, threatening human health. Ammonia gas is also one of the most harmful gases to livestock and poultry for many years. Many studies have demonstrated toxic effect of ammonia gas on animal health, such as eyes, respiratory system, and digestive system. Read More

View Article and Full-Text PDF

Membrane perturbation, altered morphology and killing of Staphylococcus epidermidis upon contact with a cytocompatible peptide-based antibacterial surface.

Colloids Surf B Biointerfaces 2021 Apr 6;203:111745. Epub 2021 Apr 6.

Department of Medicine (DAME), University of Udine, piazzale Kolbe, 4, 33100, Udine, Italy. Electronic address:

One possibility to prevent prosthetic infections is to produce biomaterials resistant to bacterial colonization by anchoring membrane active antimicrobial peptides (AMPs) onto the implant surface. In this perspective, a deeper understanding of the mode of action of the immobilized peptides should improve the development of AMP-inspired infection-resistant biomaterials. The aim of the present study was to characterize the bactericidal mechanism against Staphylococcus epidermidis of the AMP BMAP27(1-18), immobilized on titanium disks and on a model resin support, by applying viability counts, Field Emission Scanning Electron Microscopy (FE-SEM), and a fluorescence microplate assay with a membrane potential-sensitive dye. Read More

View Article and Full-Text PDF

Direct conjugation of fluorescent quantum dots with E. coli via surface-displayed histidine-containing peptides.

Colloids Surf B Biointerfaces 2021 Apr 1;203:111730. Epub 2021 Apr 1.

DEVCOM Army Research Laboratory, Biotechnology Branch, 2800 Powder Mill Road, Adelphi, MD, 20783, United States.

Biocompatible approaches to labeling bacteria with fluorescent nanoparticles are essential in order to create living bacterial bioconjugates for imaging, biosensors, medicine, and other applications. Herein we report the direct conjugation of carboxyl quantum dots (QDs) with E. coli outer membrane via surface-displayed binding peptides. Read More

View Article and Full-Text PDF

Bremerella alba sp. nov., a novel planctomycete isolated from the surface of the macroalga Fucus spiralis.

Syst Appl Microbiol 2021 Mar 15;44(3):126189. Epub 2021 Mar 15.

Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal; CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal. Electronic address:

A bacterial strain, designated FF15, was isolated from the thallus surface of the macroalga Fucus spiralis sampled on a rocky beach in Porto, Portugal. Based on the 16S rRNA gene sequence, strain FF15 was affiliated to the phylum Planctomycetes. This strain forms white colonies on modified M13 medium and the cells are pear-shaped, can form rosettes, divide by polar budding and are motile. Read More

View Article and Full-Text PDF

Insight the roles of loosely-bound and tightly-bound extracellular polymeric substances on Cu, Zn and Pb biosorption process with Desulfovibrio vulgaris.

J Colloid Interface Sci 2021 Mar 29;596:408-419. Epub 2021 Mar 29.

College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.

The aim of this study is to explore the fate and mechanism of metal cations of biosorption in the Desulfovibrio vulgaris system (including bacterial cells and secreted loosely-bound extracellular polymeric substances (LB-EPS) and tightly-bound extracellular polymeric substances (TB-EPS)). The relative contribution of EPS (TB-EPS and LB-EPS) to the adsorption of three metal cations is much greater than that of bacterial cells, and the adsorption capacity of Pb on EPS (TB-EPS and LB-EPS) is much greater than that of Cu and Zn (Pb > Cu > Zn). The order of absorption capacity was as follows: LB-EPS > TB-EPS > bacterial cells, the adsorption contribution of EPS (including TB-EPS and LB-EPS) to Cu, Zn and Pb accounted for total adsorption capacity was 82%, 83% and 86%, respectively. Read More

View Article and Full-Text PDF

Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway.

Phytomedicine 2021 Mar 27;86:153555. Epub 2021 Mar 27.

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Background: Calcium oxalate (CaOx) crystal deposition and crystal-induced renal tubular epithelial cell injury have been found to fundamentally contribute to the formation of CaOx nephrolithiasis.

Purpose: In the current work, we aim to study the role and mechanism of kaempferol in CaOx crystal kidney deposition and crystal-induced renal injury.

Study Design: Mice models and HK-2 cells were used to investigate the effect of kaempferol in CaOx crystal-induced renal injury and crystal deposition in the kidney and its underlying mechanism by a series of experiments. Read More

View Article and Full-Text PDF

Cannabidiol-dihydroartemisinin conjugates for ameliorating neuroinflammation with reduced cytotoxicity.

Bioorg Med Chem 2021 Apr 2;39:116131. Epub 2021 Apr 2.

Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; Department of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China. Electronic address:

Cannabidiol (CBD) and dihydroartemisinin (DHA) can alleviate neuroinflammatory responses. However, they show cytotoxicity, which severely limits their therapeutic windows. Therefore, there is a great need to develop neuroprotective agents with improved safety. Read More

View Article and Full-Text PDF

EZcount: An all-in-one software for microRNA expression quantification from NGS sequencing data.

Comput Biol Med 2021 Apr 3;133:104352. Epub 2021 Apr 3.

Institute for Informatics and Telematics, CNR, Pisa, 56124, Italy; Department of Computer Science, University of Pisa, Pisa, 56127, Italy. Electronic address:

MicroRNAs (miRNAs) are short endogenous molecules of RNA that influence cell regulation by suppressing genes. Their ubiquity throughout all branches of the tree of life has suggested their central role in many cellular functions. Nowadays, several personalized medicine applications rely on miRNAs as biomarkers for diagnoses, prognoses, and prediction of drug response. Read More

View Article and Full-Text PDF

Synthesis and structure-activity relationship study of novel 3-diethoxyphosphorylfuroquinoline-4,9-diones with potent antitumor efficacy.

Eur J Med Chem 2021 Apr 3;219:113429. Epub 2021 Apr 3.

Institute of Organic Chemistry, Lodz University of Technology, Żeromskiego 116, 90-924, Łódź, Poland. Electronic address:

Herein we report an efficient synthesis of a series of regioisomeric N,O-syn and N,O-anti 3-diethoxyphosphorylfuroquinoline-4,9-diones combining furoquinoline-5,8-dione skeleton, present in several highly cytotoxic compounds, with diethoxyphosphoryl moiety. The cytotoxic activity of the obtained analogs was tested against two human cancer cell lines: promyelocytic leukemia HL-60 and breast cancer adenocarcinoma MCF-7 and for comparison on human umbilical vein endothelial cells HUVEC and mammary gland/breast MCF-10 A cells. Several diethoxyphosphorylfuroquinoline-4,9-diones proved to be highly cytotoxic for cancer cells with IC values even below 0. Read More

View Article and Full-Text PDF

Characterization of CD31 expression in CD4+ and CD8+T cell subpopulations in chronic untreated HIV infection.

Immunol Lett 2021 Apr 11. Epub 2021 Apr 11.

Instituto Nacional de Enfermedades Respiratorias, Centro de Investigación en Enfermedades Infecciosas, México City, Mexico.

Background: The platelet endothelial cell adhesion molecule-1 (PECAM-1) or CD31 has been involved in regulation of T-cell tolerance, activation, survival and homing in mice cells. However, there is limited knowledge about the expression pattern and role of this molecule in human T cells, particularly in conditions of chronic immune activation.

Objectives: We explored CD31 expression in T cell differentiation subsets of individuals with untreated HIV infection and in non-HIV-infected controls. Read More

View Article and Full-Text PDF

Translation of single-cell transcriptomic analysis of uveal melanomas to clinical oncology.

Prog Retin Eye Res 2021 Apr 11:100968. Epub 2021 Apr 11.

University Côte d'Azur, France; Inserm, Biology and Pathologies of Melanocytes, Team1, Equipe Labellisée Ligue 2020 and Equipe Labellisée ARC 2019, Centre Méditerranéen de Médecine Moléculaire, Nice, France. Electronic address:

Uveal melanoma (UM) is an aggressive and deadly neoplasm. In recent decades, great efforts have been made to obtain a more comprehensive understanding of genetics, genomics and molecular changes in UM, enabling the identification of key cellular processes and signalling pathways. Still, there is no effective treatment for the metastatic disease. Read More

View Article and Full-Text PDF

Microvesicles - promising tiny players' of cancer stem cells targeted liver cancer treatments: the interesting interactions and therapeutic aspects.

Pharmacol Res 2021 Apr 11:105609. Epub 2021 Apr 11.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, 210096, China. Electronic address:

Liver cancer is one of the most malignant cancers worldwide with poor prognosis. Intracellular mediators like microvesicles (MVs) and Cancer stem cells (CSCs) are considered as potential candidates in liver cancer progression. CSCs receive stimuli from the tumor microenvironment to initiate tumor formation in which it's secreted MVs play a noteworthy role. Read More

View Article and Full-Text PDF

Liver organoid as a 3D in vitro model for drug validation and toxicity assessment.

Pharmacol Res 2021 Apr 11:105608. Epub 2021 Apr 11.

Gallipoli Medical Research Institute, Greenslopes Private Hospital, Brisbane, QLD, Australia; School of Clinical Medicine, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia. Electronic address:

The past decade has seen many advancements in the development of three-dimensional (3D) in vitro models in pharmaceutical sciences and industry. Specifically, organoids present a self-organising, self-renewing and more physiologically relevant model than conventional two-dimensional (2D) cell cultures. Liver organoids have been developed from a variety of cell sources, including stem cells, cell lines and primary cells. Read More

View Article and Full-Text PDF