5,835,251 results match your criteria cells plga-icg-r848-based


No Detectable Alteration of Inorganic Allogeneic Bone Matrix Colonizing Mesenchymal Cells: A Step Towards Personalized Bone Grafts.

J Bone Metab 2021 May 31;28(2):161-169. Epub 2021 May 31.

Université Clermont Auvergne, CHU Clermont-Ferrand, GECOM, CRB Auvergne, Clermont-Ferrand, France.

Background: During major bone substance loss, secured allogeneic bone matrix (ABM) is normally utilized for bone repair. Here, we propose a method to colonize ABM using autologous mesenchymal cells (MCs) to improve their integration. Moreover, in this study, the consequences of in vitro colonization on MCs have been evaluated. Read More

View Article and Full-Text PDF

Differentiation of Brain Tumor Microvasculature From Normal Vessels Using Optical Coherence Angiography.

Lasers Surg Med 2021 Jun 15. Epub 2021 Jun 15.

Department of Biomedical Engineering, The University of Texas Austin, Austin, Texas, 78712, USA.

Background And Objectives: Despite rapid advances and discoveries in medical imaging, monitoring therapeutic efficacy for malignant gliomas and monitoring tumor vasculature remains problematic. The purpose of this study is to utilize optical coherence angiography for vasculature characterization inside and surrounding brain tumors in a murine xenograft brain tumor model. Features included in our analysis include fractional blood volume, vessel tortuosity, diameter, orientation, and directionality. Read More

View Article and Full-Text PDF

Toward a Liver Cell Atlas: Understanding Liver Biology in Health and Disease at Single-Cell Resolution.

Semin Liver Dis 2021 Jun 15. Epub 2021 Jun 15.

Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland.

Single-cell technologies are revolutionizing our understanding of cellular heterogeneity and functional diversity in health and disease. Here, we review the current knowledge and advances in liver biology using single-cell approaches. We focus on the landscape of the composition and the function of cells in a healthy liver in the context of its spatial organization. Read More

View Article and Full-Text PDF

Interleukin-17 in Liver Disease Pathogenesis.

Semin Liver Dis 2021 Jun 15. Epub 2021 Jun 15.

Department of Surgery, University of California, San Diego, La Jolla, CA.

Interleukin 17A (IL-17A)-producing T helper 17 (Th17) cells were identified as a subset of T helper cells that play a critical role in host defense against bacterial and fungal pathogens. Th17 cells differentiate from Th0 naïve T-cells in response to transforming growth factor β1 (TGF-β1) and IL-6, the cytokines which also drive development of liver fibrosis, require activation of transcription factor retinoic acid receptor-related orphan nuclear receptor gamma (RORγ). IL-17A signals through the ubiquitously expressed receptor IL-17RA. Read More

View Article and Full-Text PDF

Human Neutrophil Elastase Mediates MUC5AC Hypersecretion via the Tumour Necrosis Factor-α Converting Enzyme-Epidermal Growth Factor Receptor Signalling Pathway in vivo.

ORL J Otorhinolaryngol Relat Spec 2021 Jun 15:1-9. Epub 2021 Jun 15.

Department of Otolaryngology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Objectives: The objective of this study is to examine the role of the tumour necrosis factor-α converting enzyme-epidermal growth factor receptor (TACE-EGFR) pathway in human neutrophil elastase (HNE)-induced MUC5AC mucin expression in mice.

Method: Four groups of mice, treated with HNE alone (HNE group), HNE plus TACE inhibitor (HNE + TAPI-2 group), HNE plus EGFR inhibitor (HNE + AG1478 group), and untreated (control group), were used in the experiment. Histopathological changes were monitored by haematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining. Read More

View Article and Full-Text PDF

Long Noncoding RNA GAS5 Targeting miR-221-3p/Cyclin-Dependent Kinase Inhibitor 2B Axis Regulates Follicular Thyroid Carcinoma Cell Cycle and Proliferation.

Pathobiology 2021 Jun 15:1-12. Epub 2021 Jun 15.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, China.

Introduction: Follicular thyroid carcinoma (FTC) is more aggressive than the most common papillary thyroid carcinoma (PTC). However, the current research on FTC is less than PTC. Here, we investigated the effects of long noncoding RNA (lncRNA) GAS5 and miR-221-3p in FTC. Read More

View Article and Full-Text PDF

Cariogenic Potential of Human and Bovine Milk on Enamel Demineralization.

Caries Res 2021 Jun 15:1-8. Epub 2021 Jun 15.

Piracicaba Dental School, UNICAMP, Piracicaba, Brazil.

The higher cariogenicity of human milk when compared with bovine milk is still a debatable subject. Therefore, we evaluated the effect of human or bovine milk exposure on biofilm composition and enamel demineralization using a validated cariogenic biofilm model. Streptococcus mutans UA159 biofilms (n = 8) were grown on human saliva-coated bovine enamel slabs of known surface hardness. Read More

View Article and Full-Text PDF

Indoleamine-2,3-Dioxygenase Activates Wnt/β-Catenin Inducing Kidney Fibrosis after Acute Kidney Injury.

Gerontology 2021 Jun 15:1-9. Epub 2021 Jun 15.

Department of Nephrology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, China.

Introduction: As disorder of tryptophan metabolism is common in CKD, the rate-limiting enzyme of tryptophan, indoleamine-2,3-dioxygenase (IDO), has been reported to be involved in CKD, while the accurate mechanism remains unknown. This study was designed to explore correlations between IDO and kidney fibrosis after ischemia-reperfusion injury (IRI).

Methods: Wild-type (WT) mice and IDO knockout (IDO-/-) mice were divided into the sham group and acute kidney injury (AKI) group. Read More

View Article and Full-Text PDF

Temporal order of mutations influences cancer initiation dynamics.

Phys Biol 2021 Jun 15. Epub 2021 Jun 15.

Department of Chemistry and Rice Quantum Institute, Rice University, 6100 Main Street, USA, Houston, Texas, 77005-1892, UNITED STATES.

Cancer is a set of genetic diseases that are driven by mutations. It was recently discovered that the temporal order of genetic mutations affects the cancer evolution and even the nature of the disease itself. The mechanistic origin of these observations, however, remain not well understood. Read More

View Article and Full-Text PDF

Demystifying the spontaneous phenomena of motor hyperexcitability.

Clin Neurophysiol 2021 May 13;132(8):1830-1844. Epub 2021 May 13.

UK Dementia Research Institute, Department of Basic and Clinical Neuroscience, Maurice Wohl Clinical Neuroscience Institute, Institute of Psychiatry, Psychology and Neuroscience, King's College London, UK; King's College Hospital NHS Foundation Trust, Denmark Hill, London, UK.

Possessing a discrete functional repertoire, the anterior horn cell can be in one of two electrophysiological states: on or off. Usually under tight regulatory control by the central nervous system, a hierarchical network of these specialist neurons ensures muscular strength is coordinated, gradated and adaptable. However, spontaneous activation of these cells and their axons can result in abnormal muscular twitching. Read More

View Article and Full-Text PDF

Lipolytic enzymes and free fatty acids at the endothelial interface.

Atherosclerosis 2021 May 28;329:1-8. Epub 2021 May 28.

Department of Medicine, Center for Human Nutrition, Washington University School of Medicine, Saint Louis, MO, USA. Electronic address:

Lipids released from circulating lipoproteins by intravascular action of lipoprotein lipase (LpL) reach parenchymal cells in tissues with a non-fenestrated endothelium by transfer through or around endothelial cells. The actions of LpL are controlled at multiple sites, its synthesis and release by myocytes and adipocytes, its transit and association with the endothelial cell luminal surface, and finally its activation and inhibition by a number of proteins and by its product non-esterified fatty acids. Multiple pathways mediate endothelial transit of lipids into muscle and adipose tissues. Read More

View Article and Full-Text PDF

Biological effects induced by doses of mammographic screening.

Phys Med 2021 Jun 12;87:90-98. Epub 2021 Jun 12.

Department of Radiological Sciences (LCR), State University of Rio de Janeiro (UERJ), Pavilhão Haroldo Lisboa da Cunha, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro, RJ CEP 20550-900, Brazil.

Purpose: Mammography is the diagnostic imaging practice used in screening to detect early lesions suspected of malignancy. It uses a low energy X-ray beam in which a low dose in the order of 2-3 mGy is delivered to patient breast cells. However, it has been speculated that it could lead to significant cell damage, when compared to conventional X-ray. Read More

View Article and Full-Text PDF

Synthesis and evaluation of trypanocidal activity of derivatives of naturally occurring 2,5-diphenyloxazoles.

Bioorg Med Chem 2021 Jun 12;42:116253. Epub 2021 Jun 12.

Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.

African trypanosomiasis is a zoonotic protozoan disease affecting the nervous system. Various natural products reportedly exhibit trypanocidal activity. Naturally occurring 2,5-diphenyloxazoles present in Oxytropis lanata, and their derivatives, were synthesized. Read More

View Article and Full-Text PDF

Progress in the study of D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) reversing multidrug resistance.

Colloids Surf B Biointerfaces 2021 Jun 8;205:111914. Epub 2021 Jun 8.

Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, PR China. Electronic address:

Currently, multidrug resistance (MDR) is one of the major reasons for failure in clinical cancer chemotherapy. Overexpression of the ATP binding cassette (ABC) transporter P-glycoprotein (P-gp), which significantly increases the efflux of anticancer drugs from tumor cells, enhances MDR. In the past few decades, four generations of P-gp inhibitors have appeared. Read More

View Article and Full-Text PDF

USP22 regulates the formation and function of placental vasculature during the development of fetal growth restriction.

Placenta 2021 Jun 9;111:19-25. Epub 2021 Jun 9.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China. Electronic address:

Introduction: Fetal growth restriction (FGR) is a common obstetric complication that can lead to a variety of adverse perinatal outcomes and is associated with chronic diseases in adulthood. Since ubiquitin-specific protease 22 (USP22) is closely related to cell growth, differentiation and proliferation, we aimed to investigate the role of USP22 in FGR development.

Methods: USP22 expression was detected in the placentas of eight normal and eight pregnant women with FGR. Read More

View Article and Full-Text PDF

Nanonewton scale adhesion force measurements on biotinylated microbeads with a robotic micropipette.

J Colloid Interface Sci 2021 Jun 3;602:291-299. Epub 2021 Jun 3.

ELTE Eötvös Loránd University, Department of Biological Physics, Budapest, Hungary; CellSorter Company for Innovations, Erdőalja út 174, H-1037 Budapest, Hungary. Electronic address:

Binding force between biomolecules has a crucial role in most biological processes. Receptor-ligand interactions transmit physical forces and signals simultaneously. Previously, we employed a robotic micropipette both in live cell and microbead adhesion studies to explore the adhesion force of biomolecules such as cell surface receptors including specific integrins on immune cells. Read More

View Article and Full-Text PDF

Combined toxicity of nano-TiO and Cd to Scenedesmus obliquus: Effects at different concentration ratios.

J Hazard Mater 2021 Jun 9;418:126354. Epub 2021 Jun 9.

Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.

The continuous release of manufactured nanomaterials (MNMs) to environments raised concerns on their combined toxicological risks with co-existing contaminants, since MNMs might severely alter the environmental behavior and fate of the contaminants. In this study, the combined toxicity of nano-sized titanium dioxide (nTiO) and cadmium (Cd) to the green alga Scenedesmus obliquus and the underlying physicochemical mechanisms were investigated for the first time at different concentration ratios of Cd to nTiO to closely mimic the realistic environment scenarios where the concentration ratios of nTiO to other contaminants are constantly changing. Our results suggested that under the co-exposure to different concentration ratios of Cd to nTiO, the co-exposure contaminants exhibited three different combined toxicity modes (antagonistic, partially additive, and synergistic). Read More

View Article and Full-Text PDF

Generation of an induced pluripotent stem cell line (UNCCi002-A) from a healthy donor using a non-integration system to study Cerebral Cavernous Malformation (CCM).

Stem Cell Res 2021 Jun 9;54:102421. Epub 2021 Jun 9.

Human Pluripotent Stem Cell Core, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Department of Genetics at the University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States. Electronic address:

The generation of induced pluripotent stem cells (iPSCs) from healthy individuals is an invaluable resource as reference control in disease modeling and drug discovery. This paper details the reprogramming of peripheral blood mononuclear cells (PBMCs) isolated from a healthy 27 years-old male using non-integration technology. The derived iPSCs displayed typical pluripotent stem cell morphology, the capacity to differentiate into the three germ layers, and normal karyotype. Read More

View Article and Full-Text PDF

Nicotinamide mononucleotide-elicited NAMPT signaling activation aggravated adjuvant-induced arthritis in rats by affecting peripheral immune cells differentiation.

Int Immunopharmacol 2021 Jun 12;98:107856. Epub 2021 Jun 12.

Department of Traditional Chinese Medicine, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu 241000, China; Research Center of Integration of Traditional Chinese and Western Medicine, Wannan Medical College, Wuhu 241000, China; Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College, Wuhu 241000, China. Electronic address:

Supplement of nicotinamide mononucleotide (NMN), the direct precursor of nicotinamide adenine dinucleotide (NAD) has gained prominence due to the significant anti-aging potentials of nicotinamide phosphoribosyltransferas (NAMPT)/NAD signaling. Because over-expression of NAMPT is deeply implicated in inflammatory arthritis, we investigated the effects of NMN supplement on rats with adjuvant-induced arthritis (AIA). Tested rats were given oral treatment of NMN at 200 mg/kg/day for 25 days. Read More

View Article and Full-Text PDF

Development and validation of a novel ferroptosis-related gene signature for predicting prognosis and immune microenvironment in head and neck squamous cell carcinoma.

Int Immunopharmacol 2021 Jun 12;98:107789. Epub 2021 Jun 12.

Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China; Department of Hearing and Speech Science, Xinhua College, Sun Yat-Sen University, Guangzhou, China. Electronic address:

Ferroptosis plays an important role across variable cancer types. However, few studies have focused on the prognostic patterns of ferroptosis-related genes in HNSCC. Cohorts with mRNA expression profiles, as well as corresponding clinical data of HNSCC patients from published studies, were collected and consolidated from public databases. Read More

View Article and Full-Text PDF

Repellent activity of acetylcarvacrol and its effects on salivary gland morphology in unfed Rhipicephalus sanguineus sensu lato ticks (Acari: Ixodidae).

Ticks Tick Borne Dis 2021 Jun 4;12(5):101760. Epub 2021 Jun 4.

Department of Health Sciences, Federal University of Lavras (UFLA), Av. Dr. Sylvio, Menicucci, 1001 Lavras, MG, Brazil. Electronic address:

Rhipicephalus sanguineus sensu lato (s.l.), commonly known as brown dog tick, is a widely distributed tick species that is substantially important for human and veterinary medicine. Read More

View Article and Full-Text PDF

A novel organ-chip system emulates three-dimensional architecture of the human epithelia and the mechanical forces acting on it.

Biomaterials 2021 Jun 6;275:120957. Epub 2021 Jun 6.

Emulate Inc., 27 Drydock Avenue, 5th Floor, Boston, MA, 02210, USA. Electronic address:

Successful translation of in vivo experimental data to human patients is an unmet need and a bottleneck in the development of effective therapeutics. Organ-on-Chip technology aims to address this need by leveraging recent significant advancements in microfabrication and biomaterials, which enable modeling of organs and their functionality. These microengineered chips offer researchers the possibility to recreate critical elements of native tissue architecture such as in vivo relevant tissue-tissue interface, air-liquid interface, and mechanical forces, including mechanical stretch and fluidic shear stress, which are crucial to recapitulate tissue level functions. Read More

View Article and Full-Text PDF

BMP2 immune complexes promote new bone formation by facilitating the direct contact between osteoclasts and osteoblasts.

Biomaterials 2021 May 16;275:120890. Epub 2021 May 16.

Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, 401147, China; Department of Oral Implantology, Stomatological Hospital of Chongqing Medical University, Chongqing, 401147, China. Electronic address:

BMP2 antibody is proposed as a promising replacement for rhBMP2 in bone tissue engineering. Although studies have demonstrated its osteoinductive efficacy, the underlying osteogenic mechanism and adverse reactions of specific BMP2 antibody are not clarified yet, making it difficult to optimize the antibody for future application. By establishing BMP2 immune complexes (BMP2-ICs) ex vivo, we were able to introduce BMP2-ICs directly in vivo and found that BMP2-ICs promoted bone formation while suppressing osteoclastogenesis. Read More

View Article and Full-Text PDF

Tumor progress intercept by intervening in Caveolin-1 related intercellular communication via ROS-sensitive c-Myc targeting therapy.

Biomaterials 2021 Jun 7;275:120958. Epub 2021 Jun 7.

College of Pharmaceutical Science, Zhejiang University, Hangzhou, 310058, China. Electronic address:

Tumor-associated macrophages (TAMs) in the tumor microenvironment (TME) play an important role in the development of tumors by secreting a variety of cytokines or directly communicating with tumor cells, making TAMs-targeted therapeutic strategies very attractive. It has been reported that oncogene c-Myc is related to every aspect of the oncogenic process of tumor cells and the alternative activation of macrophages. Hence, we constructed a glycolipid nanocarrier containing ROS-responsive peroxalate linkages (CSOPOSA) for ROS-triggered release of drugs and further modified it with Ex 26 (Ex 26-CSOPOSA), a selective sphingosine 1-phosphate receptor 1 (S1PR1) antagonist, to achieve the dual-targeted delivery of the c-Myc inhibitor JQ1 via S1PR1, which is overexpressed on both tumor cells and TAMs, thereby inducing apoptosis of tumor cells, and blocking M2 polarization of macrophages. Read More

View Article and Full-Text PDF

Alkaline thermal pretreatment of waste activated sludge for enhanced hydrogen production in microbial electrolysis cells.

J Environ Manage 2021 Jun 12;294:113000. Epub 2021 Jun 12.

State Key Laboratory of Heavy Oil Processing, Beijing Key Lab of Oil & Gas Pollution Control, China University of Petroleum, Beijing, 102249, China; College of Chemical Engineering and Environment, China University of Petroleum, Beijing, 102249, China. Electronic address:

Resource utilization of waste activated sludge (WAS) has become a mainstream development direction. Alkaline thermal pretreatment (TPT) was found to greatly promote the bioaccessibility and biodegradability of the sludge. The organic matter including soluble chemical oxygen demand (SCOD), soluble carbohydrate, soluble protein and volatile fatty acids (VFAs) after low temperature (90 °C) pretreatment was 4. Read More

View Article and Full-Text PDF

Peroxiredomin-4 ameliorates lipotoxicity-induced oxidative stress and apoptosis in diabetic cardiomyopathy.

Biomed Pharmacother 2021 Jun 12;141:111780. Epub 2021 Jun 12.

Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China. Electronic address:

Diabetic cardiomyopathy (DCM), one severe complication in the diabetes, leads to high mortality in the diabetic patients. However, the understanding of molecular mechanisms underlying DCM is far from completion. Herein, we investigated the disease-related differences in the proteomes of DCM based on db/db mice and verified the protective roles of peroxiredoxin-4 (Prdx4) in H9c2 cardiomyocytes treated by palmitic acid (PA). Read More

View Article and Full-Text PDF

Single and double modified salinomycin analogs target stem-like cells in 2D and 3D breast cancer models.

Biomed Pharmacother 2021 Jun 12;141:111815. Epub 2021 Jun 12.

Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205, United States.

Breast cancer remains one of the leading cancers among women. Cancer stem cells (CSCs) are tumor-initiating cells which drive progression, metastasis, and reoccurrence of the disease. CSCs are resistant to conventional chemo- and radio-therapies and their ability to survive such treatment enables tumor reestablishment. Read More

View Article and Full-Text PDF

Novel autoantibodies to the β-cell surface epitopes of ZnT8 in patients progressing to type-1 diabetes.

J Autoimmun 2021 Jun 12;122:102677. Epub 2021 Jun 12.

Department of Physiology, Johns Hopkins School of Medicine, Baltimore, MD, USA. Electronic address:

Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by autoimmune destruction of insulin-producing β-cells in pancreatic islets. Seroconversions to islet autoantibodies (IAbs) precede the disease onset by many years, but the role of humoral autoimmunity in the disease initiation and progression are unclear. In the present study, we identified a new IAb directed to the extracellular epitopes of ZnT8 (ZnT8ec) in newly diagnosed patients with T1D, and demonstrated immunofluorescence staining of the surface of human β-cells by autoantibodies to ZnT8ec (ZnT8ecA). Read More

View Article and Full-Text PDF

Glutamine addiction in virus-infected mammalian cells: A target of the innate immune system?

Med Hypotheses 2021 Jun 2;153:110620. Epub 2021 Jun 2.

Department of Life and Health Sciences, University of Nicosia, Nicosia, Cyprus. Electronic address:

Control of core cell metabolism is a key aspect of the evolutionary conflict between viruses and the host's defence mechanisms. From their side, the invading viruses press the accelerator on their host cell's glycolysis, fatty acid, and glutaminolytic metabolic processes among others. It is also well established that activation of innate immune system responses modulates facets of metabolism such as that of polyamine, cholesterol, tryptophan and many more. Read More

View Article and Full-Text PDF

Synthesis and pharmacological evaluation of pomalidomide derivatives useful for sickle cell disease treatment.

Bioorg Chem 2021 Jun 10;114:105077. Epub 2021 Jun 10.

São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara 14800-903, Brazil. Electronic address:

Fetal hemoglobin (HbF) induction constitutes a valuable and validated approach to treat the symptoms of sickle cell disease (SCD). Here, we synthesized pomalidomide-nitric oxide (NO) donor derivatives (3a-f) and evaluated their suitability as novel HbF inducers. All compounds demonstrated different capacities of releasing NO, ranging 0. Read More

View Article and Full-Text PDF