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Neurocoagulation from a Mechanistic Point of View in the Central Nervous System.

Semin Thromb Hemost 2022 Jan 20. Epub 2022 Jan 20.

Department of Neurology, The Chaim Sheba Medical Center, Ramat Gan, Israel.

Coagulation mechanisms are critical for maintaining homeostasis in the central nervous system (CNS). Thrombin, an important player of the coagulation cascade, activates protease activator receptors (PARs), members of the G-protein coupled receptor family. PAR1 is located on neurons and glia. Read More

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January 2022

MicroRNAs in Obesity-Associated Disorders: The Role of Exercise Training.

Obes Facts 2022 Jan 20:1-13. Epub 2022 Jan 20.

Laboratory of Biochemistry and Molecular Biology of Exercise, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil.

Obesity is a worldwide epidemic affecting over 13% of the adult population and is defined by an excess of body fat that predisposes comorbidities. It is considered a multifactorial disease in which environmental and genetic factors interact, and it is a risk marker for cardiovascular disease. Lifestyle modifications remain the mainstay of treatment for obesity based on adequate diet and physical exercise. Read More

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January 2022

Threshold of Serum Ferritin to Discriminate against Those at Greater Risk of Platelet Increase during Treatment with Hypoxia-Inducible Factor Prolyl Hydroxylase Domain Inhibitor.

Acta Haematol 2022 Jan 20. Epub 2022 Jan 20.

Introduction: Hypoxia-inducible factor prolyl hydroxylase domain inhibitors (HIF-PHI) are a new treatment for renal anemia. HIF-PHI is believed to increase iron usage to improve availability of iron for erythropoiesis. Therefore, there is concern that HIF-PHI might be prone to iron deficiency and that thrombosis might be induced by increased platelet and transferrin levels due to this iron deficiency. Read More

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January 2022

Immunological significance of survival-related alternative splicing in uveal melanoma.

Aging (Albany NY) 2022 Jan 19;14(undefined). Epub 2022 Jan 19.

Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

Uveal melanoma (UM) is a highly malignant intraocular tumor. The imbalance of alternative splicing (AS) is a landmark of tumor initiation and progression. However, there are few studies of AS in UM. Read More

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January 2022

CDX2 controls genes involved in the metabolism of 5-fluorouracil and is associated with reduced efficacy of chemotherapy in colorectal cancer.

Biomed Pharmacother 2022 Jan 17;147:112630. Epub 2022 Jan 17.

Strasbourg University, INSERM, IRFAC / UMR-S1113, FHU ARRIMAGE, FMTS, 3 avenue Molière, 67200 Strasbourg, France. Electronic address:

Most patients affected with colorectal cancers (CRC) are treated with 5-fluorouracil (5-FU)-based chemotherapy but its efficacy is often hampered by resistance mechanisms linked to tumor heterogeneity. A better understanding of the molecular determinants involved in chemoresistance is critical for precision medicine and therapeutic progress. Caudal type homeobox 2 (CDX2) is a master regulator of intestinal identity and acts as tumor suppressor in the colon. Read More

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January 2022

Tumor-microenvironmental-response Bi-functional molecules for efficient imaging and anti-tumor activity therapy.

Eur J Med Chem 2022 Jan 12;230:114120. Epub 2022 Jan 12.

School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, PR China. Electronic address:

To improve the visualization and potency of anticancer agents, the diagnosis and treatment integration bi-functional molecules were constructed based on active candidate BD7, approved drug Linifanib, and monoclonal antibody Bevacizumab. Commercial available Rhodamine B was inducted to realize imaging-aided diagnosis and target efficiency monitoring for cancer cells. In order to maintain the anticancer activity of drugs, disulfide bond was incorporated as releasable group based on tumor microenviroment. Read More

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January 2022

Harmine-based dual inhibitors targeting histone deacetylase (HDAC) and DNA as a promising strategy for cancer therapy.

Bioorg Chem 2022 Jan 12;120:105604. Epub 2022 Jan 12.

Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

Overexpression of histone deacetylases (HDACs) are observed in different types of cancers, but histone deacetylase inhibitors (HDACIs) have not shown significant efficacy as monotherapy against solid tumors. Recently, studies demonstrated that it is promising to combine HDACIs with DNA damage agents to improve DNA damage level to gain better effect on treating solid tumor. Harmine has been demonstrated to cause DNA damage by intercalating DNA. Read More

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January 2022

Immunotherapeutic HCW9218 augments anti-tumor activity of chemotherapy via NK cell mediated reduction of therapy induced senescent cells.

Mol Ther 2022 Jan 17. Epub 2022 Jan 17.

HCW Biologics Inc., Miramar, FL, 33025 USA. Electronic address:

Therapy induced senescence (TIS) in tumors and TIS cancer cells secrete proinflammatory senescence-associated secretory phenotype (SASP) factors. SASP factors promote TIS cancer cells to re-enter the growth cycle with stemness characteristics, resulting in chemo-resistance and disease relapse. Herein, we showed that the immunotherapeutic HCW9218, comprising TGF-β receptor II and IL-15/IL-15 receptor α domains, enhanced metabolic and cytotoxic activities of immune cells and reduced TIS tumor cells in vivo to improve the efficacy of docetaxel and gemcitabine plus nab-paclitaxel against B16F10 melanoma and SW1990 pancreatic tumors, respectively. Read More

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January 2022

Human olfactory epithelium-derived stem cells ameliorate histopathological deficits and improve behavioral functions in a rat model of cerebellar ataxia.

J Chem Neuroanat 2022 Jan 19;120:102071. Epub 2022 Jan 19.

Brain Mapping Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Cell replacement therapy (CRT) is one of the most effective approaches used to alleviate symptoms of neurodegenerative syndromes such as cerebellar ataxia (CA). Human olfactory epithelium mesenchymal stem cells (OE-MSCs) have been recognized as a promising candidate for CRT, due to their distinctive features including immunomodulatory properties and ease of accessible compared to other types of MSCs. Hence, the main goal of our study was to explore the impacts of OE-MSCs transplantation on behavioral, structural, and histological deficiencies in a rat model of CA. Read More

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January 2022

Targeted delivery of exosomal miR-484 reprograms tumor vasculature for chemotherapy sensitization.

Cancer Lett 2022 Jan 18;530:45-58. Epub 2022 Jan 18.

State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi'an, PR China. Electronic address:

The vascular dysfunction of ovarian cancer (OC) contributes to the chemotherapeutic resistance. In this study, we aimed to explore whether exosome-mediated angiogenesis blocking could improve the chemotherapy sensitivity via vascular normalization. Exosomes were armed with RGD on the surface by fusing Lamp2b. Read More

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January 2022

TRIM21 inhibits the osteogenic differentiation of mesenchymal stem cells by facilitating K48 ubiquitination-mediated degradation of Akt.

Exp Cell Res 2022 Jan 17;412(2):113034. Epub 2022 Jan 17.

Southern Medical University, Guangzhou, Guangdong Province, China; Second Department of Orthopaedics, Zhongshan People's Hospital, Zhongshan, Guangdong Province, China. Electronic address:

Tripartite motif containing 21 (TRIM21) is a member of the TRIM protein family with E3 ubiquitin ligase activity. Recent studies have demonstrated that TRIM21 widely contributes to physiological and pathological processes by ubiquitylating critical proteins in many kinds of cells. Additionally, multiple studies have shown that TRIM21 plays an important role in multiple cell differentiation processes. Read More

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January 2022

Prospect of CAR T-cell therapy in acute myeloid leukemia.

Expert Opin Investig Drugs 2022 Jan 24:1-10. Epub 2022 Jan 24.

Division of Hematology and Oncology, Department of Medicine, Mayo Clinic, Florida, USA.

Introduction: Long-term outcome of patients with acute myeloid leukemia (AML) remains dismal, especially for those with high-risk disease or who are refractory to conventional therapy. CAR T-cell therapy provides unique opportunity to improve outcome by specifically targeting leukemia cells through genetically engineered T cells.

Areas Covered: We summarize the progress of CAR T-cells therapy in AML. Read More

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January 2022

The Invasive Potential of Hepatoma Cells Induced by Radiotherapy is Related to the Activation of Stellate Cells and Could be Inhibited by EGCG Through the TLR4 Signaling Pathway.

Radiat Res 2022 Jan 20. Epub 2022 Jan 20.

Key Laboratory of Endoscopic Technology Research, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, P.R. China.

Post-radiotherapy recurrence and metastasis of liver cancer were thought to arise from the invasion and metastasis of residual hepatocellular carcinoma cells, but it has now been shown to be closely related to the increased metastatic potential of residual liver cancer cells mediated by radiotherapy. The changes of liver microenvironment after radiotherapy also provide a favorable condition for promoting the metastatic potential of hepatocellular carcinoma. Studies have shown that radiation-induced activation of hepatic stellate cells (HSCs) is one of the main changes in the microenvironment of hepatocellular carcinoma. Read More

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January 2022

Optimum dose of Chaetoceros for controlling methanogenesis to improve power production of microbial fuel cell.

Water Sci Technol 2022 Jan;85(1):257-264

Department of Civil Engineering, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.

The marine algae Chaetoceros contains hexadecatrienoic acid, which suppresses methanogen development and improves the coulombic efficiency (CE) of microbial fuel cells (MFC). To inhibit the methanogens, optimum concentration of marine algae should be added to the anaerobic sludge to enhance the performance of MFC. A varying concentration of Chaetoceros ranging from 1 to 20 mg/mL was carried out for pretreatment of an anaerobic-mix consortium to suppress methanogens. Read More

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January 2022

Personalized Multimodal Demarcation of Peritumoral Tissue in Glioma.

JCO Precis Oncol 2020 Nov;4:1128-1140

Mind, Brain Imaging, and Neuroethics, Institute of Mental Health Research, University of Ottawa, Ottawa, Ontario, Canada.

Purpose: Gliomas are life-threatening brain tumors, and the extent of surgical resection is one of the strongest influences on survival rate. However, the proper distinction of infiltrated tissue remains elusive. The aim of this study was to use multimodal analyses to demarcate peritumoral tissue (PT) from tumoral (TT) and healthy tissue (HT). Read More

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November 2020

1,2,4-Triazole-3-Thione Analogues with a 2-Ethylbenzoic Acid at Position 4 as VIM-type Metallo-β-Lactamase Inhibitors.

ChemMedChem 2022 Jan 20. Epub 2022 Jan 20.

Universite de Montpellier, IBMM, Pôle Chimie Balard, Campus CNRS, 34093, Montpellier, FRANCE.

Metallo-β-lactamases (MBLs) are increasingly involved as a major mechanism of resistance to carbapenems in relevant opportunistic Gram-negative pathogens. Unfortunately, clinically efficient MBL inhibitors still represent an unmet medical need .  We previously reported several series of compounds based on the 1,2,4-triazole-3-thione scaffold. Read More

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January 2022

Copper extraction from low-grade chalcopyrite in a bioleaching column assisted by bioelectrochemical system.

Environ Sci Pollut Res Int 2022 Jan 20. Epub 2022 Jan 20.

School of Civil Engineering and Architecture, Wuhan University of Technology, Wuhan, 430000, China.

Low-grade ores, tailings, and solid wastes contain small amounts of valuable heavy metals. Improper disposal of these substances results in the waste of resources and contamination of soil or groundwater. Accordingly, the treatment and recycling of low-grade ores, tailings, and solid wastes attracted much attention recently. Read More

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January 2022

Ginkgolide B inactivates the NLRP3 inflammasome by promoting autophagic degradation to improve learning and memory impairment in Alzheimer's disease.

Metab Brain Dis 2022 Feb 20;37(2):329-341. Epub 2022 Jan 20.

Department of Neurology, Xuzhou Hospital Affiliated to Jiangsu University, Xuzhou, Jiangsu, 221005, People's Republic of China.

The NLR family, pyrin domain containing 3 (NLRP3) inflammasome drives the progression of Alzheimer's disease (AD). Ginkgolide B (GB) is a potential anti-inflammatory compound that controls neuro-inflammation. The aim of this study was to evaluate the effect of GB on the NLRP3 inflammasome in AD. Read More

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February 2022

Assimilatory deficit and energy regulation in young Handroanthus chrysotrichus plants under flooding stress.

J Plant Res 2022 Jan 20. Epub 2022 Jan 20.

Department of Plant Biology, Institute of Biology, State University of Campinas-UNICAMP, Campinas, Brazil.

Flooding negatively influences the growth and development of several plant species. Here, we show that the flood tolerance of young Handroanthus chrysotrichus plants involves growth deficit, carbon assimilation reductions, starch remobilization, and energy regulation. The effect of hypoxia was evaluated in a controlled experiment consisting of plants subjected to normoxia and water-logging, with later recovery. Read More

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January 2022

Use of Fluoro-[18F]-Deoxy-2-D-Glucose Positron Emission Tomography/Computed Tomography to Predict Immunotherapy Treatment Response in Patients With Squamous Cell Oral Cavity Cancers.

JAMA Otolaryngol Head Neck Surg 2022 Jan 20. Epub 2022 Jan 20.

Department of Imaging, Dana-Farber Cancer Institute, Boston, Massachusetts.

Importance: Neoadjuvant immunotherapy is a novel approach with the potential to improve outcomes for patients with oral cavity squamous cell cancer (OCSCC). Adverse events of varying severity are reported with immunotherapy, and a biomarker to predict response would be clinically useful to avoid toxic effects in those unlikely to benefit.

Objective: To correlate changes on fluoro-[18F]-deoxy-2-D-glucose positron emission tomography/computed tomography (FDG-PET/CT) scans with primary tumor pathologic response and immunologic biomarkers in patients with OCSCC receiving neoadjuvant immunotherapy. Read More

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January 2022

High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics.

Lab Chip 2022 Jan 20. Epub 2022 Jan 20.

School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China.

On-chip concentration of rare malignant tumor cells (MTCs) in malignant pleural effusions (MPEs) with a large volume is challenging. Previous microfluidic concentrators suffer from a low concentration factor (CF) and a limited processing throughput. This study describes a low-cost multiplexed microfluidic concentrator that can enable high-throughput (up to 16 mL min) and high CF (over 40-fold for single run) concentration of rare cells from large-volume biofluids (up to hundreds of milliliters). Read More

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January 2022

Microfluidic spinning of fucoxanthin-loaded nanofibers for enhancing antioxidation and clarification of fruit juice.

Food Funct 2022 Jan 20. Epub 2022 Jan 20.

Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Gangjingzi District, Dalian 116034, Liaoning, China.

Fruit juice is one of the most popular drinks, which requires strict processing conditions to ensure its quality, especially to prevent enzymatic browning and turbidity loss. In this work, a new strategy for the preparation of composite nanofibers for juice clarification and anti-browning control was proposed. The strategy used microfluidic spinning to combine Fucoxanthin (Fx), hydroxypropyl-γ-cyclodextrin (HP-γ-CD) and polyvinyl pyrrolidone (PVP) to prepare Fx/HP-γ-CD-PVP (PCF) nanofibers, which not only reflected the excellent antioxidant properties of cyclodextrin-wrapped Fx, but also achieved a more optimized juice clarification agent dosage. Read More

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January 2022

Automated Sample Preparation and Data Collection Workflow for High-Throughput In Vitro Metabolomics.

Metabolites 2022 Jan 8;12(1). Epub 2022 Jan 8.

School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.

Regulatory bodies have started to recognise the value of in vitro screening and metabolomics as two types of new approach methodologies (NAMs) for chemical risk assessments, yet few high-throughput in vitro toxicometabolomics studies have been reported. A significant challenge is to implement automated sample preparation of the low biomass samples typically used for in vitro screening. Building on previous work, we have developed, characterised and demonstrated an automated sample preparation and analysis workflow for in vitro metabolomics of HepaRG cells in 96-well microplates using a Biomek i7 Hybrid Workstation (Beckman Coulter) and Orbitrap Elite (Thermo Scientific) high-resolution nanoelectrospray direct infusion mass spectrometry (nESI-DIMS), across polar metabolites and lipids. Read More

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January 2022

Modelling Metabolic Shifts during Cardiomyocyte Differentiation, Iron Deficiency and Transferrin Rescue Using Human Pluripotent Stem Cells.

Metabolites 2021 Dec 22;12(1). Epub 2021 Dec 22.

Faculty of Medicine and Health Sciences, Norwich Medical School, University of East Anglia, Norwich NR4 7UQ, UK.

Cardiomyocytes rely on specialised metabolism to meet the high energy demand of the heart. During heart development, metabolism matures and shifts from the predominant utilisation of glycolysis and glutamine oxidation towards lactate and fatty acid oxidation. Iron deficiency (ID) leads to cellular metabolism perturbations. Read More

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December 2021

Metabolomics Defines Complex Patterns of Dyslipidaemia in Juvenile-SLE Patients Associated with Inflammation and Potential Cardiovascular Disease Risk.

Metabolites 2021 Dec 21;12(1). Epub 2021 Dec 21.

Centre for Rheumatology Research, Department of Medicine, University College London, London W1CE 6JF, UK.

Cardiovascular disease (CVD) is a leading cause of mortality in patients with juvenile-onset systemic lupus erythematosus (JSLE) associated with atherosclerosis. The interplay between dyslipidaemia and inflammation-mechanisms that drive atherosclerosis-were investigated retrospectively in adolescent JSLE patients using lipoprotein-based serum metabolomics in patients with active and inactive disease, compared to healthy controls (HCs). Data was analysed using machine learning, logistic regression, and linear regression. Read More

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December 2021

Role of Autophagy in Cell Growth under Salinity.

Plants (Basel) 2022 Jan 12;11(2). Epub 2022 Jan 12.

Laboratory of Molecular and Ecological Physiology, Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova 2, 197376 St. Petersburg, Russia.

The microalga (formerly ) is able to accumulate high amounts of the carotenoid astaxanthin in the course of adaptation to stresses like salinity. Technologies aimed at production of natural astaxanthin for commercial purposes often involve salinity stress; however, after a switch to stressful conditions,   experiences massive cell death which negatively influences astaxanthin yield. This study addressed the possibility to improve cell survival in   subjected to salinity via manipulation of the levels of autophagy using AZD8055, a known inhibitor of TOR kinase previously shown to accelerate autophagy in several microalgae. Read More

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January 2022

Cells Lacking the Zinc Vacuolar Transporter Zrt3 Display Improved Ethanol Productivity in Lignocellulosic Hydrolysates.

J Fungi (Basel) 2022 Jan 14;8(1). Epub 2022 Jan 14.

Centre of Molecular and Environmental Biology (CBMA), Department of Biology, Campus de Gualtar, University do Minho, 4710-057 Braga, Portugal.

Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Read More

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January 2022

Impact of Diquafosol Ophthalmic Solution on Tear Film and Dry Eye Symptom in Type 2 Diabetic Dry Eye: A Pilot Study.

J Ocul Pharmacol Ther 2022 Jan 19. Epub 2022 Jan 19.

He Eye Specialist Hospital, Shenyang, China.

Diquafosol ophthalmic solution (DQS) stimulates P2Y2 receptors on the ocular surface, which enhances mucin secretion from goblet cells. Therefore, tear film stability and hydration of the ocular surface can be achieved independent from lacrimal gland function. This prospective, open-label pilot study included 60 eyes of 30 diabetic patients diagnosed with dry eye disease (DED) and were randomly assigned to either DQS ( = 30 eyes) or hyaluronate (HA) group ( = 30 eyes). Read More

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January 2022

Microfluidic Surgery in Single Cells and Multicellular Systems.

Chem Rev 2022 Jan 20. Epub 2022 Jan 20.

Department of Mechanical Engineering, Stanford University, Stanford, California 94305, United States.

Microscale surgery on single cells and small organisms has enabled major advances in fundamental biology and in engineering biological systems. Examples of applications range from wound healing and regeneration studies to the generation of hybridoma to produce monoclonal antibodies. Even today, these surgical operations are often performed manually, but they are labor intensive and lack reproducibility. Read More

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January 2022

Hyperspectral Counting of Multiplexed Nanoparticle Emitters in Single Cells and Organelles.

ACS Nano 2022 Jan 20. Epub 2022 Jan 20.

Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Nanomaterials are the subject of a range of biomedical, commercial, and environmental investigations involving measurements in living cells and tissues. Accurate quantification of nanomaterials, at the tissue, cell, and organelle levels, is often difficult, however, in part due to their inhomogeneity. Here, we propose a method that uses the distinct optical properties of a heterogeneous nanomaterial preparation in order to improve quantification at the single-cell and organelle level. Read More

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January 2022