22,576 results match your criteria cells immunohistochemically

Giant Pilomatrical Tumor With Broad Epidermal Components: An Example of Histological Diversity and a Potential Diagnostic Pitfall of Tumors With Pilomatrical Differentiation.

Am J Dermatopathol 2021 Jul 20. Epub 2021 Jul 20.

Departments of Diagnostic Pathology, and Dermatology, Kyoto University Hospital, Kyoto, Japan; Department of Dermatology, Kagawa University Hospital, Kagawa, Japan; and National Hospital Organization Kyoto Medical Center, Kyoto, Japan.

Abstract: The diagnosis of pilomatricoma, the most common matrical tumor, is generally straightforward; however, it exhibits diverse histology associated with various morphological stages and several clinical variants, and matrical differentiation can occur in various neoplastic diseases. A 56-year-old man was admitted to our hospital to resect an 11.0-cm skin tumor on his right shoulder. Read More

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Hypopharyngeal Ulcers in COVID-19: Histopathological and Virological Analyses - A Case Report.

Front Immunol 2021 5;12:676828. Epub 2021 Jul 5.

Department of Neuroscience, Section of Human Anatomy, University of Padova, Padova, Italy.

In coronavirus disease 2019 (COVID-19), ulcerative lesions have been episodically reported in various segments of the gastrointestinal (GI) tract, including the oral cavity, oropharynx, esophagus, stomach and bowel. In this report, we describe an autopsy case of a COVID-19 patient who showed two undiagnosed ulcers at the level of the anterior and posterior walls of the hypopharynx. Molecular testing of viruses involved in pharyngeal ulcers demonstrated the presence of severe acute respiratory syndrome - coronavirus type 2 (SARS-CoV-2) RNA, together with herpes simplex virus 1 DNA. Read More

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Spontaneous granulocytic leukemia in a NOD/Shi- IL-2Rγ mouse.

J Toxicol Pathol 2021 Jul 18;34(3):241-244. Epub 2021 Apr 18.

CMIC Pharma Science Co., Ltd., 10221 Kobuchisawa-cho, Hokuto-shi, Yamanashi 408-0044, Japan.

Here, we report a case of spontaneous granulocytic leukemia in a 51-week-old male NOD/Shi- IL-2R (NOG) mouse. The mouse showed progressive anemia and rough respiratory movement. Macroscopically, the spleen was discolored and enlarged. Read More

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Single-cell image analysis reveals a protective role for microglia in glioblastoma.

Neurooncol Adv 2021 Jan-Dec;3(1):vdab031. Epub 2021 May 4.

Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.

Background: Microglia and tumor-associated macrophages (TAMs) constitute up to half of the total tumor mass of glioblastomas. Despite these myeloid populations being ontogenetically distinct, they have been largely conflated. Recent single-cell transcriptomic studies have identified genes that distinguish microglia from TAMs. Read More

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The Immune Microenvironment of Chordomas: An Immunohistochemical Analysis.

Cancers (Basel) 2021 Jul 2;13(13). Epub 2021 Jul 2.

Pathology Department, University Hospital of Saint-Etienne, 42055 Saint-Etienne, France.

Chordomas are rare sarcomas that are usually treated by surgery and/or radiotherapy since these are chemo-resistant tumors, but immunotherapy could be a possible option for chordoma patients. However, few reports investigating the composition of the chordoma immune microenvironment exist. We immunohistochemically studied 81 chordomas regarding their immune microenvironment factors and compared them with clinicopathological data. Read More

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Serum metallothionein - a potential oncomarker?

Z Horakova I Starek

Bratisl Lek Listy 2021 ;122(8):577-581

Introduction: Metallothionein's (MT) overexpression has been demonstrated immunohistochemically in neoplastic cells of many tumour types. Its elevation above the physiological level has been confirmed in circulation of their hosts. The results of studies dealing with the topic have been summarized to verify if this marker can be applied in the current oncologic practise. Read More

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as a Diagnostic Biomarker for Cervical Squamous Cell Carcinoma.

Genet Test Mol Biomarkers 2021 Jul;25(7):463-470

Department of Gynecology, The Second Hospital of Tianjin Medical University, Tianjin, China.

, a newly discovered member of the immunoglobulin superfamily, exerts antitumor effects by binding to receptor on natural killer cells; it has important clinical implications. Cell surface ectodomain shedding of generates soluble B7-H6 (), which is highly expressed and serves as a valuable biomarker in multiple tumors, but the clinical significance and diagnostic value of in cervical squamous cell carcinoma (CSCC) remains unclear. To assess the expression and diagnostic value of in CSCC. Read More

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Banxia xiexin decoction affects drug sensitivity in gastric cancer cells by regulating MGMT expression via IL‑6/JAK/STAT3‑mediated PD‑L1 activity.

Int J Mol Med 2021 Aug 19;48(2). Epub 2021 Jul 19.

Department of Gastroenterology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China.

Banxia xiexin decoction (BXXX) is a classic preparation used to treat gastrointestinal diseases, and also has certain therapeutic effects on gastrointestinal tumors. BXXX has been reported to regulate the expression of proteins associated with drug resistance and sensitivity in tumors, and thus, the aim of the present study was to investigate the mechanisms of BXXX drug sensitivity in gastric cancer (GC). The expression levels of programmed cell death 1 ligand 1 (PD‑L1), 6‑O‑methylguanine‑DNA methyltransferase (MGMT) and STAT3 were immunohistochemically detected in the cancer and adjacent non‑cancer tissues of patients with GC, and experimentation was conducted using drug‑resistant and ‑sensitive GC cells. Read More

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Ossifying fibromyxoid tumor of the oral cavity: rare case report and long-term follow-up.

Autops Case Rep 2021 8;11:e2020216. Epub 2020 Dec 8.

Universidade Estadual de Campinas (UNICAMP), Faculdade de Odontologia de Piracicaba, Departamento de Diagnóstico Oral, Área de Semiologia e Patologia Oral, Piracicaba, SP, Brasil.

Ossifying fibromyxoid tumor (OFMT) is a rare mesenchymal soft tissue benign neoplasm with an uncertain line of differentiation, which arises most frequently in extremities. The head and neck region involvement is uncommon, with only ten intraoral cases published in the English-language literature. One additional case of OFMT is reported here, including a literature review of intraoral reported cases. Read More

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December 2020

Single Institutional Experience on Orbital Inflammatory Pseudotumor: Diagnostic and Management Challenge.

Balkan Med J 2021 Jul;38(4):239-243

Department of Pathology and Laboratory Medicine, MedStar Georgetown University Hospital, USA.

Aims: Orbital inflammatory pseudotumor is considered a non-neoplastic inflammatory process. The finding of clonality of B or T-cell receptors in cases pathologically diagnosed as orbital inflammatory pseudotumor has unknown clinicopathologic significance. We sought to investigate potential B and T-cell clonality and concomitant diseases in cases pathologically diagnosed as orbital inflammatory pseudotumor. Read More

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Human and rodent red blood cells do not demonstrate xanthine oxidase activity or XO-Catalyzed nitrite reduction to NO.

Free Radic Biol Med 2021 Jul 14. Epub 2021 Jul 14.

West Virginia University Departments of Physiology and Pharmacology, USA. Electronic address:

A number of molybdopterin enzymes, including xanthine oxidoreductase (XOR), aldehyde oxidase (AO), sulfite oxidase (SO), and mitochondrial amidoxime reducing component (mARC), have been identified as nitrate and nitrite reductases. Of these enzymes, XOR has been the most extensively studied and reported to be a substantive source of nitric oxide (NO) under inflammatory/hypoxic conditions that limit the catalytic activity of the canonical NOS pathway. It has also been postulated that XOR nitrite reductase activity extends to red blood cell (RBCs) membranes where it has been immunohistochemically identified. Read More

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Feline pyloric and duodenal adenoma: A histological and immunohistochemical study.

Vet Pathol 2021 Jul 16:3009858211030542. Epub 2021 Jul 16.

12143The University of Tokyo, Tokyo, Japan.

Although pyloric and duodenal adenomas occasionally occur in cats, limited information is currently available on their phenotypes and molecular features. The present study investigated the pathological features of these tumors and the mechanisms underlying their tumorigenesis. Biopsy samples from 8 cats diagnosed with pyloric or duodenal adenomas were examined by histopathology and immunohistochemistry. Read More

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Reproductive tract neoplasia in adult female Asian elephants ().

Vet Pathol 2021 Jul 16:3009858211031843. Epub 2021 Jul 16.

7114San Diego Zoo Global, San Diego, CA, USA.

Recent reports have highlighted a lower-than-expected prevalence of neoplasia in elephants and suggested mechanisms for cancer resistance. But despite infrequent reports in the literature, uterine neoplasia is common in managed Asian elephants (). This study is an archival review of reproductive tract neoplasia in 80 adult female Asian elephant mortalities in managed care facilities in the United States from 1988 to 2019. Read More

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Expression of SOX2 and OCT4 in odontogenic cysts and tumors.

Head Face Med 2021 Jul 14;17(1):29. Epub 2021 Jul 14.

Faculty of Dentistry, Chulalongkorn University, Henri-Dunant Road, Pathumwan, 10330, Bangkok, Thailand.

Background: Aberrant expression of stem cell markers has been observed in several types of neoplasms. This trait attributes to the acquired stem-like property of tumor cells and can impact patient prognosis. The objective of this study was to comparatively analyze the expression and significance of SOX2 and OCT4 in various types of odontogenic cysts and tumors. Read More

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A case of intracranial myxoid mesenchymal tumor with EWSR1:CREM fusion in an adult female: Extensive immunohistochemical evaluation.

Neuropathology 2021 Jul 13. Epub 2021 Jul 13.

Division of Neurosurgery, Department of Brain and Neurosciences, Faculty of Medicine, Tottori University, Tottori, Japan.

Intracranial myxoid mesenchymal tumor (IMMT) is a recently described, extremely rare group of neoplasms characterized by fusions between the female-expressed transcript (FET) family genes and the cAMP response element-binding protein (CREB) family genes. Controversy persists regarding whether the tumor is a myxoid variant of angiomatoid fibrous histiocytoma or a completely distinct clinicopathological entity. Here, we report a case of IMMT arising in the posterior fossa in a 65-year-old woman with a history of breast cancer. Read More

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Primary central nervous system lymphomas with massive intratumoral hemorrhage: Clinical, radiological, pathological, and molecular features of six cases.

Neuropathology 2021 Jul 13. Epub 2021 Jul 13.

Department of Pathology, School of Health Sciences, Fujita Health University, Toyoake, Japan.

Primary central nervous system lymphomas (PCNSLs) rarely exhibit intratumoral hemorrhage. The differential diagnosis of hemorrhagic neoplasms of the central nervous system (CNS) currently includes metastatic carcinomas, melanomas, choriocarcinomas, oligodendrogliomas, and glioblastomas. Here we present the clinical, radiological, pathological, and molecular genetic features of six cases of PCNSL associated with intratumoral hemorrhage. Read More

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Comprehensive immunomolecular profiling of endometrial carcinoma: A tertiary retrospective study.

Gynecol Oncol 2021 Jul 9. Epub 2021 Jul 9.

Department of Human Genetics, University Hospitals Leuven, Leuven, Belgium.

Objective: Combined immunohistochemical and molecular classification using the Proactive Molecular Risk Classifier for Endometrial Cancer (ProMisE) independently predicts prognosis in endometrial carcinoma (EC). As next-generation sequencing (NGS) is entering clinical practice, we evaluated whether more comprehensive immunomolecular profiling (CIMP), including NGS and extended immunohistochemical analysis, could further refine the current ProMisE classification.

Methods: A series of 120 consecutive ECs, classified according to ProMisE, was stained immunohistochemically for CD3, CD8, PD-L1, beta-catenin and L1CAM. Read More

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Combining Multiplex Fluorescence in situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections.

J Vis Exp 2021 Jun 25(172). Epub 2021 Jun 25.

Department of Pharmacology, School of Medical Sciences, University of New South Wales;

Fluorescent in situ hybridization (FISH) is a molecular technique that identifies the presence and spatial distribution of specific RNA transcripts within cells. Neurochemical phenotyping of functionally identified neurons usually requires concurrent labelling with multiple antibodies (targeting protein) using immunohistochemistry (IHC) and optimization of in situ hybridization (targeting RNA), in tandem. A "neurochemical signature" to characterize particular neurons may be achieved however complicating factors include the need to verify FISH and IHC targets before combining the methods, and the limited number of RNAs and proteins that may be targeted simultaneously within the same tissue section. Read More

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Adenomatoid Tumor of the Adrenal Gland: Report of Two Cases and Review of the Literature.

Front Endocrinol (Lausanne) 2021 23;12:692553. Epub 2021 Jun 23.

Department of Pathology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Adenomatoid tumor (AT) is an uncommon benign neoplasm of mesothelial origin, usually occurring in the female and male genital tracts. Extragenital localization such as the adrenal gland is extremely rare. Until now, only 39 cases of adrenal AT have been reported in the English literature. Read More

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Overexpression of claspin promotes docetaxel resistance and is associated with prostate-specific antigen recurrence in prostate cancer.

Cancer Med 2021 Jul 9. Epub 2021 Jul 9.

Department of Molecular Pathology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Although docetaxel (DTX) confers significant survival benefits in patients with castration-resistant prostate cancer (CRPC), resistance to DTX inevitably occurs. Therefore, clarifying the mechanisms of DTX resistance may improve survival in patients with CRPC. Claspin plays a pivotal role in DNA replication stress and damage responses and is an essential regulator for the S-phase checkpoint. Read More

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Evaluation of E-Cadherin and β-Catenin Immunoreactivity for Determining Undifferentiated Cells in Anaplastic Thyroid Carcinoma.

Pathobiology 2021 Jul 8:1-8. Epub 2021 Jul 8.

Department of Surgery, Kuma Hospital, Kobe, Japan.

Introduction: An immunohistochemical study has occasionally been performed to diagnose anaplastic thyroid carcinoma (ATC). However, antibodies to confirm the undifferentiated nature of ATC have not yet been evaluated. The aim of this study was to evaluate E-cadherin and β-catenin expressions in immunoreactivity to determine undifferentiated carcinoma cells in the diagnosis of ATC. Read More

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Effect of low-intensity pulsed ultrasound on orofacial sensory disturbance following inferior alveolar nerve injury: Role of neurotrophin-3 signaling.

Eur J Oral Sci 2021 Jul 8:e12810. Epub 2021 Jul 8.

Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan.

Percutaneous treatment of low-intensity pulsed ultrasound (LIPUS) to the site of inferior alveolar nerve (IAN) transection promotes functional regeneration, but the detailed mechanism is unknown. We examined the involvement of neurotrophin-3 (NT-3), which primarily binds with tropomyosin receptor kinase C (TrkC), in functional transected IAN regeneration following LIPUS treatment in rats. Daily LIPUS treatment to the transected IAN was performed, and the mechanical sensitivity of the facial skin was measured for 14 d. Read More

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Novel detection of the CAMTA1-WWTR1 fusion gene in extra-adrenal myelolipoma-like lesion: a case report.

Virchows Arch 2021 Jul 6. Epub 2021 Jul 6.

Division of Pathology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.

A mediastinal mass was incidentally detected by chest X-ray in a 44-year-old man. Computed tomography findings revealed that the mass was a possible malignancy in the right and middle mediastinum and was removed by surgical resection. Macroscopically, the resected specimen was a well-demarcated yellowish, brownish, and whitish mass. Read More

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The Prognostic Significance of Plakophilin-1 Expression in Esophageal Cancer.

Anticancer Res 2021 Jul;41(7):3401-3407

First Department of Surgery, Dokkyo Medical University, Mibu, Japan.

Background/aim: Plakophilin 1 (PKP1) expression is inversely related to cancer grade. This study aimed to evaluate whether PKP1 is a prognostic marker for esophageal cancer (EC).

Materials And Methods: We tested immunohistochemically for PKP1 in squamous cell carcinoma EC specimens from 99 patients, including cytoplasmic (C), membrane (M), and nuclear (N) cellular areas, and analyzed their relationships with clinicopathological factors. Read More

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TACC3 Promotes Gastric Carcinogenesis by Promoting Epithelial-mesenchymal Transition Through the ERK/Akt/cyclin D1 Signaling Pathway.

Anticancer Res 2021 Jul;41(7):3349-3361

Department of Pathology, Chonnam National University Research Institute of Medical Science, Chonnam National University Medical School and Hwasun Hospital, Jeollanam-do, Republic of Korea;

Background/aim: The present study investigated the oncogenic functions of TACC3 in the progression of gastric cancer (GC).

Materials And Methods: We analysed TACC3 in relation to cell growth, invasion capability, expression of epithelial-mesenchymal transition (EMT)-related markers, and ERK/Akt/cyclin D1 signaling factors. The correlation between the immunohistochemically confirmed expression of TACC3 and clinical factors was also analyzed. Read More

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Mechanobiology of conjunctival epithelial cells exposed to wall shear stresses.

Biomech Model Mechanobiol 2021 Jul 6. Epub 2021 Jul 6.

Sackler Faculty of Medicine, Tel Aviv University, 69978, Tel Aviv, Israel.

The human conjunctival epithelial cells (HCEC) line the inner sides of the eyelids and the anterior part of the sclera. They include goblet cells that secret mucus into the tear film that protects the ocular surface. The conjunctival epithelium is subjected to mechano-physical stimuli due to eyelid movement during blinking, during wiping and rubbing the eyes, and when exposed to wind and air currents. Read More

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SATB2 Immunoexpression in Peripheral Ossifying Fibroma and Peripheral Odontogenic Fibroma.

Head Neck Pathol 2021 Jul 5. Epub 2021 Jul 5.

Department of Diagnostic Sciences, University of Tennessee Health Science Center, College of Dentistry, 875 Union Avenue, Memphis, TN, 38163, USA.

Peripheral ossifying fibromas (POFs) and peripheral odontogenic fibromas (POdFs) appear clinically similar but of different histogenesis. The novel marker SATB2 is involved in regulation of osteoblastic differentiation and phenotype. However, SATB2 expression has not been previously explored in POFs and POdFs. Read More

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Self-Regulation of Cerebral Metabolism and Its Neuroprotective Effect After Hypoxic-Ischemic Injury: Evidence From H-MRS.

Front Neuroanat 2021 17;15:672412. Epub 2021 Jun 17.

Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, China.

H-MRS technology can be used to non-invasively detect the content of cerebral metabolites, to assess the severity of hypoxic-ischemic (HI) injury, and to predict the recovery of compromised neurological function. However, changes to the cerebral self-regulation process after HI are still unclear. This study investigated the changes in cerebral metabolites and the potential relationship with the number of neurons and neural stem/progenitor cells (NSPC) using H-MRS, and finally clarifies the self-regulation of cerebral metabolism and neuroprotection after HI injury. Read More

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An ectopic adrenocortical oncocytic adenoma in the liver highly mimicking hepatocellular carcinoma: case report and literature review.

Diagn Pathol 2021 Jul 4;16(1):58. Epub 2021 Jul 4.

Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100730, Beijing, China.

Background: Ectopic adrenocortical tissue is a lesion usually found incidentally during autopsy or inguinal surgery. Here, we demonstrate an extremely unusual case of intrahepatic adrenocortical adenoma which highly mimicks hepatocellular carcinoma (HCC) and brings challenges for clinicians and pathologists. The diagnostic pitfalls have been discussed in detail to provide clues for guiding differential diagnosis and future treatment. Read More

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Treatment following hysteroscopy and endometrial diagnostic biopsy increases chance for live birth in women with chronic endometritis.

Am J Reprod Immunol 2021 Jul 4:e13482. Epub 2021 Jul 4.

Division of Gynecological Endocrinology and Reproductive Medicine, Bern University Hospital and University of Bern, Bern, Switzerland.

Problem Repeated implantation failure and recurrent pregnancy loss are associated with chronic endometritis, a persistent endometrial inflammation. Its diagnosis and treatment may increase pregnancy and live birth rates. The aim of this study was to assess the effectiveness of endometrial diagnostic biopsy and subsequent antibiotic treatment in cases of chronic endometritis on reproductive outcomes over a long observation period. Read More

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