65 results match your criteria cells hsc2


Wnt/β-catenin signaling pathway participates in the effect of miR-626 on oral squamous cell carcinoma by targeting RASSF4.

J Oral Pathol Med 2021 Jun 14. Epub 2021 Jun 14.

Department of Stomatology, Yantaishan Hospital, Yantai, China.

Background: The role of miR-626 in oral squamous cell carcinoma (OSCC) was investigated by targeting RASSF4.

Methods: The miR-626 and RASSF4 expression was detected in normal oral mucosa or OSCC tissues and OSCC or normal cells. The methylation status of RASSF4 was analyzed using methylation-specific polymerase chain reaction (PCR). Read More

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Gene knockout in highly purified mouse hematopoietic stem cells by CRISPR/Cas9 technology.

J Immunol Methods 2021 Aug 4;495:113070. Epub 2021 May 4.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China. Electronic address:

The CRISPR/Cas9 system has been used for genome editing of human and mouse cells. In this study, we established a protocol for gene knockout (KO) in mouse hematopoietic stem cells (HSCs). HSCs were highly purified from the bone marrow of tamoxifen-treated Cas9-EGFP/Cre-ER transgenic mice, maintained in serum-free polyvinyl alcohol culture with cytokines, lentivirally transduced with sgRNA-Crimson, and transplanted into lethally irradiated mice with competitor cells. Read More

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Micellar Casein and Whey Powder Hold a TGF-β Activity and Regulate ID Genes In Vitro.

Molecules 2021 Jan 19;26(2). Epub 2021 Jan 19.

Department of Oral Biology, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, Austria.

Casein and whey being food supplements have been considered to be used in oral health care products. However, the response of oral cells to micellar casein and whey powder remains unclear. Considering that milk contains the growth factor TGF-β, and lactoperoxidase was recently reported to decrease the expression of inhibitor of DNA-binding (ID) proteins, there is a rationale to assume that casein and whey can also provoke these responses in oral cells. Read More

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January 2021

Effects of an elemental diet, Elental®, may differ between healthy oral cells and oral cancer cells.

Oncol Rep 2021 02 11;45(2):738-751. Epub 2020 Dec 11.

Department of Oral and Maxillofacial Surgery, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755‑8505, Japan.

The effectiveness of an elemental diet (ED), Elental®, against radiotherapy‑ or chemoradiotherapy‑induced oral mucositis was previously reported. However, the administration of additional nutrition or an ED in patients with oral cancer may also provide extra nutrition for cancer cells, which could result in cancer development. At present, it remains unclear whether the beneficial effects of an ED are likely to surpass its potential harmful effects on oral cancer treatment. Read More

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February 2021

A novel TGF-β receptor II mutation (I227T/N236D) promotes aggressive phenotype of oral squamous cell carcinoma via enhanced EGFR signaling.

BMC Cancer 2020 Nov 27;20(1):1163. Epub 2020 Nov 27.

Department of Biochemistry, Research Center for Aging and Geriatrics, Research Institute of Medical Sciences, Chonnam National University Medical School, Hwasun, 58128, Republic of Korea.

Background: Transforming growth factor-β (TGF-β) signaling is a double-edged sword in cancer development and progression. TGF-β signaling plays a tumor suppressive role during the early stages of tumor development but promotes tumor progression in later stages. We have previously identified various mutations of TGF-β receptor II (TβRII) in human oral squamous cell carcinoma (OSCC) samples. Read More

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November 2020

Anti‑EpCAM monoclonal antibody exerts antitumor activity against oral squamous cell carcinomas.

Oncol Rep 2020 Dec 13;44(6):2517-2526. Epub 2020 Oct 13.

Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Aoba‑ku, Sendai, Miyagi 980‑8575, Japan.

The epithelial cell adhesion molecule (EpCAM) is a calcium‑independent, homophilic, intercellular adhesion factor classified as a transmembrane glycoprotein. In addition to cell adhesion, EpCAM also contributes to cell signaling, differentiation, proliferation, and migration. EpCAM is an essential factor in the carcinogenesis of numerous human cancers. Read More

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December 2020

A defucosylated anti‑CD44 monoclonal antibody 5‑mG2a‑f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma.

Oncol Rep 2020 Nov 14;44(5):1949-1960. Epub 2020 Aug 14.

Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Aoba‑ku, Sendai, Miyagi 980‑8575, Japan.

CD44 is widely expressed on the surface of most tissues and all hematopoietic cells, and regulates many genes associated with cell adhesion, migration, proliferation, differentiation, and survival. CD44 has also been studied as a therapeutic target in several cancers. Previously, an anti‑CD44 monoclonal antibody (mAb), C44Mab‑5 (IgG1, kappa) was established by immunizing mice with CD44‑overexpressing Chinese hamster ovary (CHO)-K1 cells. Read More

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November 2020

[Effects of propofol on TGF-β1 induced activation of hepatic stellate cells and its mechanism].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2020 May;36(3):273-278

Department of Anesthesiology, First Hospital Affiliated, University of South China, Hengyang 421001, China.

To observe the effects of propofol on the activation of hepatic stellate cell line HSC2-T6 induced by transforming growth factor-beta 1 (TGF-β1) and explore its possible mechanism. The cells were divided into control group, TGF-β1 group, propofol group, TGF-β1 + propofol group, rapamycin group, TGF-β1 + propofol + rapamycin group. Cells were treated with rapamycin (5 μmol/L) for 1 hour, propofol (100 μmol/L) for 1 hour, then TGF-β1 (5 ng/ml) was added to co-culture for 24 hours. Read More

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Anti‑EGFR monoclonal antibody 134‑mG2a exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma.

Int J Mol Med 2020 Oct 10;46(4):1443-1452. Epub 2020 Aug 10.

Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980‑8575, Japan.

The epidermal growth factor receptor (EGFR), a transmembrane receptor and member of the human epidermal growth factor receptor (HER) family of receptor tyrosine kinases, is a critical mediator of cell growth and differentiation. EGFR forms homo‑ or heterodimers with other HER family members to activate downstream signaling cascades in a number of cancer cells. In a previous study, the authors established an anti‑EGFR monoclonal antibody (mAb), EMab‑134, by immunizing mice with the ectodomain of human EGFR. Read More

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October 2020

Involvement of RNase 7 in the malignant potential of oral squamous cell carcinoma cells.

Oncol Rep 2020 Sep 7;44(3):1216-1223. Epub 2020 Jul 7.

Department of Human Biology and Pathophysiology, Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061‑0293, Japan.

RNase 7 is involved in the innate immunity of the oral epithelium. Variations in the expression levels of RNase 7 have been reported in cutaneous squamous cell carcinoma, but not in oral squamous cell carcinoma (OSCC). The present study investigated the expression levels of RNase 7 in OSCC and its role in the malignant potential of these cells. Read More

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September 2020

Milk lactoperoxidase decreases ID1 and ID3 expression in human oral squamous cell carcinoma cell lines.

Sci Rep 2020 04 3;10(1):5836. Epub 2020 Apr 3.

Department of Oral Biology, Medical University of Vienna, Sensengasse 2a, 1090, Vienna, Austria.

Milk consumption may modify the risk of squamous cell carcinoma. The role of milk to modulate the gene expression in oral squamous cell carcinoma cells has not been investigated so far. Here, HSC2 oral squamous carcinoma cells were exposed to an aqueous fraction of human milk and a whole-genome array was performed. Read More

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Domain structures of mucosa-associated lymphoid tissue lymphoma translocation 1 protein for nuclear localization in oral carcinoma cells and the proliferation inhibition.

Biochem Biophys Res Commun 2020 02 29;522(3):799-804. Epub 2019 Nov 29.

Division of Oral and Maxillofacial Surgery 2, Department of Diagnostic and Therapeutic Sciences, School of Dentistry, Meikai University, Keyakidai 1-1, Sakado, Saitama, 350-0283, Japan. Electronic address:

Mucosa-associated lymphoid tissue lymphoma translocation 1 protein (MALT1) consisting of death domain, Ig-like domains and caspase-like domain is expressed in nucleus of oral carcinoma cells, and loss of the expression closely associates with disease progression and stimulates proliferation of the cells. However, nothing is known about the molecular backgrounds. In this study, eight constructs with different domain constitution of human MALT1 and six constructs were transiently and stably transfected into oral carcinoma cell lines, respectively. Read More

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February 2020

Differential expression of claudin‑4, occludin, SOX2 and proliferating cell nuclear antigen between basaloid squamous cell carcinoma and squamous cell carcinoma.

Mol Med Rep 2019 Aug 24;20(2):1977-1985. Epub 2019 Jun 24.

Department of Pathology, Wakayama Medical University School of Medicine, Wakayama 641‑8509, Japan.

Basaloid squamous cell carcinomas (BSCCs) in oral lesions are extremely rare, and the histology is not well understood. Histologically, they are often similar to conventional squamous cell carcinoma (SCC). The present study was designed with an aim to distinguish BSCC from SCC using claudin‑4, occludin, SRY‑box 2 (SOX2) and proliferating cell nuclear antigen (PCNA) immunoreactivities and staining patterns. Read More

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Mouse acute leukemia develops independent of self-renewal and differentiation potentials in hematopoietic stem and progenitor cells.

Blood Adv 2019 02;3(3):419-431

State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

The cell of origin, defined as the normal cell in which the transformation event first occurs, is poorly identified in leukemia, despite its importance in understanding of leukemogenesis and improving leukemia therapy. Although hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) were used for leukemia models, whether their self-renewal and differentiation potentials influence the initiation and development of leukemia is largely unknown. In this study, the self-renewal and differentiation potentials in 2 distinct types of HSCs (HSC1 [CD150CD41CD34LineageSca-1c-Kit cells] and HSC2 [CD150CD41CD34LineageSca-1c-Kit cells]) and 3 distinct types of HPCs (HPC1 [CD150CD41CD34LineageSca-1c-Kit cells], HPC2 [CD150CD41CD34LineageSca-1c-Kit cells], and HPC3 [CD150CD41CD34LineageSca-1c-Kit cells]) were isolated from adult mouse bone marrow, and examined by competitive repopulation assay. Read More

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February 2019

Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3.

Oncol Rep 2019 Jan 11;41(1):333-340. Epub 2018 Oct 11.

Department of Dental Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 Plus, Chonbuk National University, Jeonju, Jeollabuk 54896, Republic of Korea.

Licochalcone H (LCH) is a chemical compound that is a positional isomer of licochalcone C (LCC), a chalconoid isolated from the root of Glycyrrhiza inflata, which has various pharmacological properties including anti‑inflammatory, antioxidant, antitumor, and anticancer effects. However, the efficacy of LCH on cancer cells has not been investigated. The present study examined the effects of LCH on cell proliferation, induction of apoptosis, and the regulation of matrin 3 (Matr3) protein in oral squamous cell carcinoma (OSCC) cells by Annexin V/propidium iodide (PI) staining and western blot analysis. Read More

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January 2019

The anti-inflammatory effect of milk and dairy products on periodontal cells: an in vitro approach.

Clin Oral Investig 2019 Apr 20;23(4):1959-1966. Epub 2018 Sep 20.

Department of Oral Biology, Medical University of Vienna, Sensengasse 2a, 1190, Vienna, Austria.

Objective: Milk can reduce intestinal tissue damage in colitis models, and protects infants against necrotizing enterocolitis. However, whether milk can decrease inflammation related to peri-implantitis and oral mucosal dehiscence remains unclear. We therefore investigated whether or not milk and fermented by-products have any anti-inflammatory effects on the cells of the oral cavity. Read More

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Treatment with Lactobacillus Retards the Tumor Growth of Head and Neck Squamous Cell Carcinoma Cells Inoculated in Mice.

Tohoku J Exp Med 2018 08;245(4):269-275

Department of Agricultural Chemistry, Tokyo University of Agriculture.

Bacteria have been used for more than a century to treat solid tumors. Because solid tumors generate an anaerobic environment, we evaluated the anti-tumor effect of the obligate anaerobe strain KK378, derived from Lactobacillus casei (L. casei), using mice bearing head and neck cancer. Read More

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Molecular mechanism of inhibitory effects of bovine lactoferrin on the growth of oral squamous cell carcinoma.

PLoS One 2018 30;13(1):e0191683. Epub 2018 Jan 30.

Department of Oral & Maxillofacial Pathobiology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Background: Lactoferrin (LF), a member of the transferrin family, recently has been demonstrated to have anticancer effects on various cancers including oral squamous cell carcinoma (OSCC). However, little is known about the underlying mechanisms of its effects on OSCC. Therefore, we aimed to investigate the mechanism of the suppressive effects of bovine LF (bLF) on the growth of OSCC cells. Read More

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Transcription factor EB influences invasion and migration in oral squamous cell carcinomas.

Oral Dis 2018 Jul 17;24(5):741-748. Epub 2018 Apr 17.

Department of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

Objective: Transcription factor EB (TFEB) is a master regulator of lysosomal biogenesis and plays an important role in various cancers. However, the function of TFEB in oral squamous cell carcinomas has not been examined. The aim of this study was to elucidate the role of TFEB in oral squamous cell carcinomas. Read More

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The crucial role of SEMA3F in suppressing the progression of oral squamous cell carcinoma.

Cell Mol Biol Lett 2017 28;22:32. Epub 2017 Dec 28.

Department of Stomatology, Tianjin First Center Hospital, Tianjin, 300192 People's Republic of China.

Background: Oral squamous cell carcinoma (OSCC) is one of the most common types of malignancy. Semaphorin 3F (SEMA3F) is highly conserved but present at a lower level in various cancers than in healthy tissues. While it has been reported that SEMA3F is involved in cancer cell proliferation, migration and invasion, its function in OSCC remains unknown. Read More

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Roles of VEGF-Flt-1 signaling in malignant behaviors of oral squamous cell carcinoma.

PLoS One 2017 17;12(11):e0187092. Epub 2017 Nov 17.

Department of Oral and Maxillofacial Pathobiology, School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Background: Vascular endothelial growth factor (VEGF) is a highly specific signaling protein for vascular endothelial cells that plays a critical role in tumor growth and invasion through angiogenesis, and may contribute to cell migration and activation of pre-osteoclasts, osteoclasts and some tumor cells.

Objectives: We aimed to clarify the detailed roles of VEGF-Flt-1 signaling in bone invasion of oral squamous cell carcinoma (OSCC) cells.

Results: Forty-two (42) of 54 cases with gingival SCC (77. Read More

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December 2017

Gimeracil enhances the antitumor effect of cisplatin in oral squamous cell carcinoma cells and .

Oncol Lett 2017 Sep 18;14(3):3349-3356. Epub 2017 Jul 18.

Department of Oral and Maxillofacial Surgery, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan.

Gimeracil or 5-chloro-2,4-dihydroxypyridine (CDHP) enhances the antitumor effects of 5-fluorouracil (5-FU) by inhibiting dihydropyrimidine dehydrogenase (DPD), which is involved in the degradation of 5-FU. CDHP, as part of a combination therapy, was also reported to exert a radiosensitizing effect. Therefore, CDHP may have underlying mechanisms of action other than DPD inhibition. Read More

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September 2017

Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.

Int J Oncol 2017 May 23;50(5):1634-1646. Epub 2017 Mar 23.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515, Japan.

The tumor protein D52 (TPD52) protein family includes TPD52, -53, -54 and -55. Several reports have shown important roles for TPD52 and TPD53, and have also suggested the potential involvement of TPD54, in D52-family physiological effects. Therefore, we performed detailed expression analysis of TPD52 family proteins in oral squamous cell carcinoma (OSCC). Read More

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Gimeracil Exerts Radiosensitizing Effects on Oral Squamous Cell Carcinoma Cells In Vitro and In Vivo.

Anticancer Res 2016 11;36(11):5923-5930

Department of Oral and Maxillofacial Surgery Yamaguchi University Graduate School of Medicine, Ube, Japan.

Background/aim: Gimeracil or 5-chloro-2, 4-dihydroxypyridine (CDHP) has been reported to exert radiosensitization effects in cancer cells by suppressing DNA repair pathways. Here, we investigated the antitumor effect of gimeracil and radiation combination therapy against oral squamous cell carcinoma (OSCC).

Material And Methods: The antitumor activity of gimeracil and/or radiation was investigated in HSC2 and/or SAS cells by growth inhibition assays and clonogenic survival assay. Read More

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November 2016

Wnt/Beta-Catenin Signal Inhibitor HC-1 Sensitizes Oral Squamous Cell Carcinoma Cells to 5-Fluorouracil through Reduction of CD44-Positive Population.

Yonago Acta Med 2016 Jun 29;59(2):93-9. Epub 2016 Jun 29.

Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.

Background: Oral squamous cell carcinoma is a prevalent and frequently lethal malignancy worldwide. Existence of treatment-resistant cancer stem cells is considered to be associated with tumor formation, recurrence and metastasis. Wnt/beta-catenin signal is one of the crucial signaling pathways for cancer stem cells. Read More

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Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors.

BMC Cancer 2016 07 27;16:548. Epub 2016 Jul 27.

Department of Oral and Maxillofacial Surgery, Yamaguchi University Graduate School of Medicine, 1-1-1, Minamikogushi, Ube, 755-8505, Japan.

Background: The cancer stem cells (CSCs), a small subpopulation of cells in tumor are responsible for the tumor initiation, growth, recurrence and metastasis of cancer, as well as resistance of cancers to drugs or radiotherapy. CSCs are an important target for the development of novel strategies in cancer treatment. However, CSCs-targeted new anti-cancer drug discovery is currently hindered by the lack of easy and reliable methods for isolating, collecting and maintaining sufficient number of CSCs. Read More

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Clinicopathological implications of vascular endothelial growth factor 165b expression in oral squamous cell carcinoma stroma.

Oncol Rep 2016 Jul 20;36(1):573-81. Epub 2016 May 20.

Division of Craniomaxillofacial Surgery, Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Tokyo 145-8515, Japan.

Vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. VEGF165b was recently isolated as the anti-angiogenic VEGF splice variant. In the present study, we examined the association between VEGF165b expression and clinicopathological characteristics in order to determine how VEGF165b produced from oral squamous cell carcinoma (OSCC) affects the stromal cell biological activity. Read More

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Metformin in combination with 5-fluorouracil suppresses tumor growth by inhibiting the Warburg effect in human oral squamous cell carcinoma.

Int J Oncol 2016 Jul 16;49(1):276-84. Epub 2016 May 16.

Department of Oral and Maxillofacial Surgery, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan.

Cancer cells show enhanced glucose consumption and lactate production even in the presence of abundant oxygen, a phenomenon known as the Warburg effect, which is related to tumor proliferation, progression and drug-resistance in cancers. Hypoxia-inducible factor-1 (HIF-1) and several members of Phosphatidylinositol-4, 5-bisphosphate 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signaling pathway positively contribute to the Warburg effect, whereas AMP activated protein Kinase (AMPK) acts as a negative regulator. Targeting the regulator molecules of Warburg effect might be a useful strategy to effectively kill cancer cells. Read More

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MALT1 Inhibition of Oral Carcinoma Cell Invasion and ERK/MAPK Activation.

J Dent Res 2016 Apr 23;95(4):446-52. Epub 2015 Dec 23.

Department of Biochemistry, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan

The expression of mucosa-associated lymphoid tissue 1 (MALT1) that activates nuclear factor (NF)-κB in lymphocyte lineages is rapidly inactivated in oral carcinoma cells at the invasive front and the patients with worst prognosis. However, its mechanism to accelerate carcinoma progression remains unknown, and this study was carried out to examine the role in invasion. HSC2 oral carcinoma cells stably expressing wild-type MALT1 (wtMALT1) reduced the invasion of basement membrane matrices and collagen gels, and the dominant-negative form (∆MALT1)-expressing cells aggressively invaded into collagen gels. Read More

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Esculetin Induces Apoptosis Through EGFR/PI3K/Akt Signaling Pathway and Nucleophosmin Relocalization.

J Cell Biochem 2016 May 18;117(5):1210-21. Epub 2015 Oct 18.

Department of Dental Pharmacology, School of Dentistry, BK21 Plus, Chonbuk National University, Jeonju, Republic of Korea.

Esculetin, a coumarin compound, has anti-proliferative effects on various types of human cancer cells, but its effect on oral squamous cell carcinoma (OSCC) is unknown. In this study, we determined whether esculetin had anti-proliferative effects on two oral squamous cell lines, HN22, and HSC2. We found that esculetin inhibited cell viability by inducing apoptosis, as evinced by apoptotic cell morphologies, nuclear fragmentation, and the multi-caspase/MMP activity. Read More

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