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Effects of different doses of melatonin on rat adipose derived mesenchymal stem cells.

Horm Mol Biol Clin Investig 2021 Aug 4. Epub 2021 Aug 4.

Department of Histology and Embryology, Pamukkale University, School of Medicine, Denizli, Turkey.

Objectives: Stem cell treatment is based on Melatonin which is crucial for lots of pathological and physiological pathways. Our aim is determining the most appropriate dose of melatonin affecting the rat adipose tissue mesenchymal stem cells.

Methods: Stem cells were isolated from male rat adipose tissue. Read More

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Cyclic growth of dermal papilla and regeneration of follicular mesenchymal components during feather cycling.

Development 2021 Sep 3;148(18). Epub 2021 Aug 3.

Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.

How dermis maintains tissue homeostasis in cyclic growth and wounding is a fundamental unsolved question. Here, we study how dermal components of feather follicles undergo physiological (molting) and plucking injury-induced regeneration in chickens. Proliferation analyses reveal quiescent, transient-amplifying (TA) and long-term label-retaining dermal cell (LRDC) states. Read More

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September 2021

Spatial Transcriptomics analysis of uterine gene expression in enhancer of Zeste homolog 2 (Ezh2) conditional knockout mice.

Biol Reprod 2021 Aug 3. Epub 2021 Aug 3.

Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.

Histone proteins undergo various modifications that alter chromatin structure, including addition of methyl groups. Enhancer of homolog 2 (EZH2), is a histone methyltransferase that methylates lysine residue 27, and thereby, suppresses gene expression. EZH2 plays integral role in the uterus and other reproductive organs. Read More

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COVID-19 and the Kidney: Should Nephrologists Care about COVID-19 rather than Maintaining Their Focus on Renal Patients?

Contrib Nephrol 2021 Aug 3;199:1-15. Epub 2021 Aug 3.

Istituto di Ricerche Farmacologiche Mario Negri, IRCCS, Bergamo, Italy.

Clinical Background: The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly spread globally from late 2019, reaching pandemic proportions. Epidemiology: The related disease, COVID-19, exacerbates and progresses due to patients' abnormal inflammatory/immune responses, widespread endothelial damage, and complement-induced blood clotting with microangiopathy. COVID-19 manifests mainly as a respiratory illness. Read More

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YAP1 Overexpression Is Associated with Kidney Dysfunction in Lupus Nephritis.

Pathobiology 2021 Aug 3:1-12. Epub 2021 Aug 3.

Department of Pathophysiology, Guizhou Medical University, Guiyang, China.

Objective: The goal of the present study was to determine the expression of yes-associated protein 1 (YAP1) in renal tissues of mice with lupus nephritis (LN) and elucidate its role in the progression of renal fibrosis.

Methods: C57BL/6 mice and MRL/lpr mice were selected for experimental comparison. Mouse kidney tissues were removed and sectioned for hematoxylin and eosin staining, Masson's trichome staining, Sirius staining, and immunohistochemistry. Read More

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Multigene Combined Detection by RT-qPCR Using Cytological Specimens.

Acta Cytol 2021 Aug 3:1-10. Epub 2021 Aug 3.

Department of Cytology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.

Objective: The aim of the study was to investigate the mutation status of multiple driver genes by RT-qPCR and their significance in advanced lung adenocarcinoma using cytological specimens.

Materials And Methods: 155 cytological specimens that had been diagnosed with lung adenocarcinoma in the Fourth Hospital of Hebei Medical University were selected from April to November 2019. The cytological specimens included serous cavity effusion and fine-needle aspiration biopsies. Read More

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Herbal Nephropathy.

Contrib Nephrol 2021 Aug 3;199:1-12. Epub 2021 Aug 3.

Departamento de Nefrología y Metabolismo Mineral, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.

An estimated one-third of adults in developed countries and more than 80% of the population in many low- and middle-income countries use herbal and traditional medicines to promote health or for the treatment of common diseases. Herbal medicines can cause kidney damage as a result of intrinsic toxicity, adulteration, contamination, replacement, misidentification, mistaken labeling, and unfavorable herb-drug interactions. The kidneys, due to their high blood flow rate, large endothelial surface area, high metabolic activity, active uptake by tubular cells, medullary interstitial concentration, and low urine pH are particularly vulnerable to development of toxic injury in the form of different syndromes like acute kidney injury, nephrolithiasis, chronic interstitial fibrosis, or uroepithelial cancer. Read More

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PD-1 inhibitor combined with apatinib modulate the tumor microenvironment and potentiate anti-tumor effect in mice bearing gastric cancer.

Int Immunopharmacol 2021 Jul 31;99:107929. Epub 2021 Jul 31.

Department of Medical Oncology, Fujian Medical University Union Hospital, Fuzhou, Fujian, 350001, People's Republic of China. Electronic address:

Objective: To explore the effect of programmed death 1 (PD-1) inhibitor combined with apatinib on immune regulation and efficacy of the combined therapy in mice bearing gastric cancer (MBGC), and to provide a research basis for enhancing the benefit of immunotherapy in advanced gastric cancer (AGC).

Methods: MBGC were divided into normal saline group (group NS), apatinib group (group A), PD-1 inhibitors group (group B) and PD-1 inhibitors combined with apatinib group (group C). Tumor inhibition rates were calculated. Read More

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Does Chrysin prevent severe lung damage in Hyperoxia-Induced lung injury Model?

Int Immunopharmacol 2021 Jul 31;99:108033. Epub 2021 Jul 31.

Department of Pediatrics, Inonu University School of Medicine, Malatya, Turkey.

Background: Oxidative stress and inflammation play a critical role in the etiopathogenesis of bronchopulmonary dysplasia (BPD). The aim of this study was to evaluate the preventive effect of Chrysin (CH), an antioxidant, antiinflammatory, antiapoptotic and antifibrotic drug, on hyperoxia-induced lung injury in a neonatal rat model.

Methods: Forty infant rats were divided into four groups labeled the Control, CH, BPD, and BPD + CH. Read More

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HSPA12A improves endothelial integrity to attenuate lung injury during endotoxemia through activating ERKs and Akt-dependent signaling.

Int Immunopharmacol 2021 Jul 31;99:107987. Epub 2021 Jul 31.

Department of Anesthesiology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. Electronic address:

Acute lung injury (ALI) is a critical manifestation of sepsis/septic shock. Disruption of endothelial barrier function is critical for ALI pathogenesis; however, the regulation of endothelial barrier integrity remains largely unclear. Heat shock protein A12A (HSPA12A) is an atypical member of HSP70 family. Read More

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Tracking cell migration by cellular force footprint recorded with a mechano-optical biosensor.

Biosens Bioelectron 2021 Jul 27;193:113533. Epub 2021 Jul 27.

Department of Physics and Astronomy, Iowa State University, Ames, IA, 50011, USA; Molecular, Cellular, and Development Biology Interdepartmental Program, Iowa State University, Ames, IA, 50011, USA. Electronic address:

Conventional cell migration assays require time-lapse imaging of live cells to trace cell migration paths, consequently demanding cumbersome hardware setup and suffering from low data throughput. In this work, we developed an assay named Tracking Cells by Footprint (TCF) based on a mechano-optical biosensor that irreversibly becomes fluorescent when sensing local cell adhesive force. Cell migration paths are visualized and recorded as fluorescent footprints on glass or elastic substrates coated with such biosensor. Read More

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Secondary organic aerosols produced from photochemical oxidation of secondarily evaporated biomass burning organic gases: Chemical composition, toxicity, optical properties, and climate effect.

Environ Int 2021 Jul 30;157:106801. Epub 2021 Jul 30.

Department of Earth and Planetary Sciences, Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address:

Biomass burning (BB) is an important source of primary organic aerosols (POA). These POA contain a significant fraction of semivolatile organic compounds, and can release them into the gas phase during the dilution process in transport. Such evaporated compounds were termed "secondarily evaporated BB organic gases (SBB-OGs)" to distinguish them from the more studied primary emissions. Read More

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Intensified phenol and p-cresol biodegradation for wastewater treatment in countercurrent packed-bed column bioreactors.

Chemosphere 2021 Jul 29;286(Pt 2):131716. Epub 2021 Jul 29.

Department of Chemical Engineering, Laval University, Québec, G1V 0A6, Canada. Electronic address:

An intensified biodegradation process in packed-bed column bioreactors (PBCB) with microbial cells attached to packing/micro-particles surface was proposed and simulated via a dynamic 3D model with continuity, momentum, and species (comprising biomicro-particles) balance equations in liquid/gas, accumulation of biomicro-particles in packed-bed, diffusion and enzymatic reaction within biofilm and liquid film. Phenol and phenol/p-cresol biological removal by Pseudomonas putida was chosen to discuss the enhanced biodegradation efficiency. The biodegradation in single/dual-substrate systems is significantly upgraded as biomass loading on micro-particles surface increases. Read More

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HO-mediated autophagy during ethanol metabolism.

Redox Biol 2021 Jul 24;46:102081. Epub 2021 Jul 24.

Center for Alcohol Research and Salem Medical Center, University of Heidelberg, Heidelberg, Germany. Electronic address:

Background: Alcoholic liver disease (ALD) is the most common liver disease worldwide and its underlying molecular mechanisms are still poorly understood. Moreover, conflicting data have been reported on potentially protective autophagy, the exact role of ethanol-metabolizing enzymes and ROS.

Methods: Expression of LC3B, CYP2E1, and NOX4 was studied in a mouse model of acute ethanol exposure by immunoblotting and immunohistochemistry. Read More

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Increased P2×2 receptors induced by amyloid-β peptide participates in the neurotoxicity in alzheimer's disease.

Biomed Pharmacother 2021 Jul 31;142:111968. Epub 2021 Jul 31.

Laboratorio de Screening de Compuestos Neuroactivos, Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile; Centro de Investigaciones Avanzadas en Biomedicina (CIAB-UdeC), Universidad de Concepción, Concepción, Chile. Electronic address:

Amyloid beta peptide (Aβ) is tightly associated with the physiopathology of Alzheimer's Disease (AD) as one of the most important factors in the evolution of the pathology. In this context, we previously reported that Aβ increases the expression of ionotropic purinergic receptor 2 (P2×2R). However, its role on the cellular and molecular Aβ toxicity is unknown, especially in human brain of AD patients. Read More

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Omega-3 fatty acid rescues ischaemia/perfusion-induced testicular and sperm damage via modulation of lactate transport and xanthine oxidase/uric acid signaling.

Biomed Pharmacother 2021 Jul 31;142:111975. Epub 2021 Jul 31.

Bioresources Development Centre, National Biotechnology Development Agency, Ministry of Science and Technology, Ogbomoso, Oyo State, Nigeria. Electronic address:

This study aimed to explore the potential antioxidant, anti-inflammatory, and anti-apoptotic effects of omega 3 fatty acid (Ω-3) in a rat model of testicular torsion/detorsion (T/D). Under ketamine/xylazine anaesthesia, age-matched adult male Wistar rats of comparable weight underwent sham-operation or testicular torsion by fixing the left testis rotated at 720° for two and half hours. After detorsion, animals were treated with either olive oil as vehicle or Ω-3 subcutaneously for three days. Read More

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Astrocytes constitute the major TNF-α-producing cell population in the infarct cortex in dMCAO rats receiving intravenous MSC infusion.

Biomed Pharmacother 2021 Jul 31;142:111971. Epub 2021 Jul 31.

Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing, China. Electronic address:

Recent studies report that inhibiting TNF-α might be a novel therapeutic strategy for managing brain ischemia. Our previous study reported that mesenchymal stem cell (MSC) transplantation could suppress TNF-α level in both serum and brain. However, the cell type(s) that contribute to the production of TNF-α during ischemia following MSC transplantation has not been well studied. Read More

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Emerging roles for IL-6 family cytokines as positive and negative regulators of ectopic lymphoid structures.

Cytokine 2021 Jul 31;146:155650. Epub 2021 Jul 31.

School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK. Electronic address:

IL-6 family cytokines display broad effects in haematopoietic and non-haematopoietic cells that regulate immune homeostasis, host defence, haematopoiesis, development, reproduction and wound healing. Dysregulation of these activities places this cytokine family as important mediators of autoimmunity, chronic inflammation and cancer. In this regard, ectopic lymphoid structures (ELS) are a pathological hallmark of many tissues affected by chronic disease. Read More

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Effect of bamboo sphere amendment on the organic matter decomposition and humification of food waste composting.

Waste Manag 2021 Jul 30;133:19-27. Epub 2021 Jul 30.

School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; Key Laboratory of Biometallurgy, Ministry of Education, Changsha 410083, China. Electronic address:

The aim of this study is to examine the effect of bamboo sphere on the organic matter decomposition and humification of food waste composting. Food waste composting were carried out on four treatments, namely control (CK), 3% (T1), 6% (T2) and 9% (T3) (w/w) bamboo sphere treatments. Results showed that adding bamboo sphere facilitated the organic matter decomposition and increased the seed germination index. Read More

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Neutrophils aid cellular therapeutics by enhancing glycoengineered stem cell recruitment and retention at sites of inflammation.

Biomaterials 2021 Jul 27;276:121048. Epub 2021 Jul 27.

Division of Cardiovascular Medicine and the Clinical and Translational Research Center, University at Buffalo, State University of New York, Buffalo, NY, 14260, USA; Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY, 14260, USA; Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY, 14260, USA; Department of Medicine, University at Buffalo, State University of New York, Buffalo, NY, 14260, USA. Electronic address:

The efficacy of cell-based therapies relies on targeted payload delivery and enhanced cell retention. In vitro and in vivo studies suggest that the glycoengineering of mesenchymal and cardiosphere-derived cells (CDCs) may enhance such recruitment at sites of injury. We evaluated the role of blood cells in amplifying this recruitment. Read More

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R-loop modulated epigenetic regulation in T helper cells mechanistically associates coronary artery disease and non-small cell lung cancer.

Transl Oncol 2021 Jul 31;14(10):101189. Epub 2021 Jul 31.

Department of Biotechnology, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203, India. Electronic address:

The effect of epigenetics in coronary artery disease and Non-small cell lung cancer (NSCLC) is presently developing as a significant vital participant at various levels from pathophysiology to therapeutics. We would like to find out the conjunction of some regular epigenetic regulations which decides the example of either acetylation/deacetylation or methylation/demethylation on various gene promoters associated with their pathogenesis. Expressions of some of the genes (e. Read More

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A deep learning approach to quantify auditory hair cells.

Hear Res 2021 Jul 22;409:108317. Epub 2021 Jul 22.

Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel 4031, Switzerland; Clinic for Otorhinolaryngology, Head and Neck Surgery, University of Basel Hospital, Petersgraben 4, Basel CH-4031, Switzerland. Electronic address:

Hearing loss affects millions of people worldwide. Yet, there are still no curative therapies for sensorineural hearing loss. Frequent causes of sensorineural hearing loss are due to damage or loss of the sensory hair cells, the spiral ganglion neurons, or the synapses between them. Read More

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A time-resolved transcriptome landscape of the developing mouse ovary.

Biochem Biophys Res Commun 2021 Jul 31;572:57-64. Epub 2021 Jul 31.

Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, China. Electronic address:

The ovarian structure is complex and diverse, including egg cells, granulosa cells and other cell types. Its organ function depends heavily on normal development. Numerous studies have focused on certain gene function changes during ovarian development, but systematic analyses of its molecular changes are extremely rare. Read More

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miR-96-5p is the tumor suppressor in osteosarcoma via targeting SYK.

Biochem Biophys Res Commun 2021 Jul 31;572:49-56. Epub 2021 Jul 31.

Department of Orthopedics, Chengdu Second People's Hospital, Chengdu, 610017, Sichuan, China.

Background: Osteosarcoma is a multiple malignant tumor in adolescents. MicroRNAs (MiRNAs) have been found to express abnormally in OS tissues and are considered as potential targets for OS prognosis and treatment.

Methods: MiR-96-5p and SYK expression in clinical samples, osteoblast and OS cell lines were detected. Read More

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Set7/9 controls proliferation and genotoxic drug resistance of NSCLC cells.

Biochem Biophys Res Commun 2021 Jul 31;572:41-48. Epub 2021 Jul 31.

Institute of Cytology, Russian Academy of Sciences, 194064, St Petersburg, Russian Federation; Moscow Institute of Physics and Technology, Dolgoprudny, 141700, Moscow Region, Russian Federation. Electronic address:

The SET domain containing lysine-specific methyltransferase, Set7/9, covalently attaches methyl moieties to a variety of histone and non-histone substrates. Among the substrates of Set7/9 are: p53, NF-kB, PARP1, E2F1, and other transcription factors that regulate many vital processes in the cell. Through the post-translational regulation of these critical master-regulators Set7/9 is involved in regulation of cell proliferation, cancer progression, and DNA damage response. Read More

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Establishment of two homozygous CRISPR interference (CRISPRi) knock-in human induced pluripotent stem cell (hiPSC) lines for titratable endogenous gene repression.

Stem Cell Res 2021 Jul 27;55:102473. Epub 2021 Jul 27.

Institute of Pharmacology and Toxicology, University Medical Center Goettingen (UMG), Göttingen, Germany; DZHK (German Center for Cardiovascular Research), partner site Göttingen, Germany. Electronic address:

Using nuclease-deficient dead (d)Cas9 without enzymatic activity fused to transcriptional inhibitors (CRISPRi) allows for transcriptional interference and results in a powerful tool for the elucidation of developmental, homeostatic and disease mechanisms. We inserted dCas9KRAB (CRISPRi) cassette into the AAVS1 locus of hiPSC lines, which resulted in homozygous knock-in with an otherwise unaltered genome. Expression of dCas9KRAB protein, pluripotency and the ability to differentiate into all three embryonic germ layers were validated. Read More

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Establishment of a KCNQ1 homozygous knockout human embryonic stem cell line by episomal vector-based CRISPR/Cas9 system.

Stem Cell Res 2021 Jul 22;55:102467. Epub 2021 Jul 22.

Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China. Electronic address:

As a member of the voltage-gated potassium ion channels, KCNQ1 plays an important role in heart physiological functions. Numerous mutations in KCNQ1 were identified as primary causes to hereditary long-QT syndrome. To further study the role of KCNQ1 in human cardiac functions, here we generated a homozygous KCNQ1 knockout human embryonic stem cell line (KCNQ1-KO) using episomal vector-based CRISPR/Cas9 system. Read More

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Comparison of the biological and functional characteristics of mesenchymal stem cells from intrahepatic and identical bone marrow.

Stem Cell Res 2021 Jul 27;55:102477. Epub 2021 Jul 27.

Hepatobiliary Institute, Southwest Hospital, the Army Medical University, No 30 Gaotanyan, ShapingBa Distract, Chongqing 400038, China. Electronic address:

In our privious work, our reseach group characterized a population of hepatic-sourced mesenchymal stem cells (MSCs) called MLpvNG2 cells. In the present study, we compared the biological and functional characteristics of naïve MLpvNG2 cells with identical bone marrow-derived MSCs (niBM-MSCs) using in vitro (conditioned media) and in vivo (a well-set diethylnitrosamine (DEN)-induced liver fibrotic/cirrhotic murine model) procedures. The intrahepatic-sourced mesodermal MLpvNG2 cells exhibited some biological characteristics (e. Read More

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Electrical vagus nerve stimulation as a prophylaxis for SIRS and postoperative ileus.

Auton Neurosci 2021 Jul 29;235:102857. Epub 2021 Jul 29.

Department of General, Visceral, Thoracic and Vascular Surgery, University Hospital of Bonn, Bonn, Germany.

Abdominal surgery results in an activation of immune cells of the bowel wall and a consecutive cytokine and nitric oxide (NO) release leading to an inflammation of the muscularis externa and a bowel paralysis, the so-called postoperative ileus (POI). In addition to the local inflammation, major surgical trauma can also lead to a variable pronounced systemic inflammation up to its maximum variant, the systemic inflammatory response syndrome (SIRS), with hypotension, capillary leak and a breakdown of the intestinal barrier function followed by multi-organ dysfunction (MODS). Until now, neither for SIRS nor for POI, a prophylaxis or an evidence-based treatment exists. Read More

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Role of heparanase 2 (Hpa2) in gastric cancer.

Neoplasia 2021 Jul 31;23(9):966-978. Epub 2021 Jul 31.

Rappaport Faculty of Medicine, Technion Integrated Cancer Center, Technion, Haifa, Israel. Electronic address:

Heparanase is highly implicated in tumor metastasis due to its capacity to cleave heparan sulfate and, consequently, remodel the extracellular matrix underlying epithelial and endothelial cells. In striking contrast, only little attention was given to its close homolog, heparanase 2 (Hpa2), possibly because it lacks heparan sulfate-degrading activity typical of heparanase. We subjected sections of gastric carcinoma to immunostaining and correlated Hpa2 immunoreactivity with clinical records, including tumor grade, stage and patients' status. Read More

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