77 results match your criteria cellobiohydrolase increasing


Using milk vetch (Astragalus sinicus L.) to promote rice straw decomposition by regulating enzyme activity and bacterial community.

Bioresour Technol 2021 Jan 6;319:124215. Epub 2020 Oct 6.

Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:

The present study determined the dynamic changes of enzyme activity and bacterial community in rice straw (RS) and milk vetch (MV) co-decomposing process. Results showed that mixing RS and MV promoted decomposition. The mixture enhanced β-glucosidase and β-cellobiohydrolase activities relative to its monospecific residue during the mid-late stage of decomposition. Read More

View Article and Full-Text PDF
January 2021

Earthworms accelerate rice straw decomposition and maintenance of soil organic carbon dynamics in rice agroecosystems.

PeerJ 2020 17;8:e9870. Epub 2020 Sep 17.

Institute of Eco-Environmental and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Background: To promote straw degradation, we inoculated returned farmland straw with earthworms (). Increasing the number of earthworms may generally alter soil organic carbon (SOC) dynamics and the biological activity of agricultural soils.

Methods: We performed soil mesocosm experiments with and without earthworms to assess the decomposition and microbial mineralization of returned straw and soil enzyme activity across different time periods. Read More

View Article and Full-Text PDF
September 2020

Synergistic Action of a Lytic Polysaccharide Monooxygenase and a Cellobiohydrolase from in Cellulose Saccharification under High-Level Substrate Loading.

Appl Environ Microbiol 2020 11 10;86(23). Epub 2020 Nov 10.

Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India

Lytic polysaccharide monooxygenases (LPMOs) are crucial industrial enzymes required in the biorefinery industry as well as in the natural carbon cycle. These enzymes, known to catalyze the oxidative cleavage of glycosidic bonds, are produced by numerous bacterial and fungal species to assist in the degradation of cellulosic biomass. In this study, we annotated and performed structural analysis of an uncharacterized LPMO from (PfLPMO9) based on computational methods in an attempt to understand the behavior of this enzyme in biomass degradation. Read More

View Article and Full-Text PDF
November 2020

Improved catalytic activity and stability of cellobiohydrolase (Cel6A) from the Aspergillus fumigatus by rational design.

Protein Eng Des Sel 2020 09;33

Department of Biotechnology, National Institute of Technology Durgapur 713209, West Bengal, India.

Cheap production of glucose is the current challenge for the production of cheap bioethanol. Ideal protein engineering approaches are required for improving the efficiency of the members of the cellulase, the enzyme complex involved in the saccharification process of cellulose. An attempt was made to improve the efficiency of the cellobiohydrolase (Cel6A), the important member of the cellulase isolated from Aspergillus fumigatus (AfCel6A). Read More

View Article and Full-Text PDF
September 2020

Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.

J Biol Chem 2020 10 18;295(43):14606-14617. Epub 2020 Aug 18.

Department of Functional Molecular Science, School of Physical Sciences, SOKENDAI (The Graduate University for Advanced Studies), Hayama, Kanagawa, Japan; Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki, Aichi, Japan. Electronic address:

Cellobiohydrolases directly convert crystalline cellulose into cellobiose and are of biotechnological interest to achieve efficient biomass utilization. As a result, much research in the field has focused on identifying cellobiohydrolases that are very fast. Cellobiohydrolase A from the bacterium (CfCel6B) and cellobiohydrolase II from the fungus (TrCel6A) have similar catalytic domains (CDs) and show similar hydrolytic activity. Read More

View Article and Full-Text PDF
October 2020

Improved Production of Majority Cellulases in by Integration of Gene From .

Front Microbiol 2020 14;11:1633. Epub 2020 Jul 14.

National Joint Engineering Research Center of Industrial Microbiology and Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou, China.

Lignocellulose is an abundant waste resource and has been considered as a promising material for production of biofuels or other valuable bio-products. Currently, one of the major bottlenecks in the economic utilization of lignocellulosic materials is the cost-efficiency of converting lignocellulose into soluble sugars for fermentation. One way to address this problem is to seek superior lignocellulose degradation enzymes or further improve current production yields of lignocellulases. Read More

View Article and Full-Text PDF

Conversion of cropland to natural vegetation boosts microbial and enzyme activities in soil.

Sci Total Environ 2020 Nov 8;743:140829. Epub 2020 Jul 8.

Dept. of Soil Science of Temperate Ecosystems, Dept. of Agricultural Soil Science, University of Göttingen 37073, Germany; Agro-Technological Institute, RUDN University, Moscow 117198, Russia.

The trends of enzyme activities and litter chemistry after abandonment of arable soil and succession of natural vegetation were studied in a deciduous forest zone (Moscow region, Russia). The Luvic Phaeozem chronosequence included an arable field, 3 fields with increasing abandonment periods (7, 11, 35 years), and the soil under natural forest as a reference site (never used for cropland). The activities of four hydrolytic enzymes (β-glucosidase, cellobiohydrolase, β-galactosidase, chitinase) in the topsoil (0-5 cm) were compared with chemical functional groups of plant litter identified by C NMR spectra. Read More

View Article and Full-Text PDF
November 2020

[Effects of Biochar Application on Soil Microbial Nutrient Limitations and Carbon Use Efficiency in Lou Soil].

Huan Jing Ke Xue 2020 May;41(5):2425-2433

Agrotechnical Promotion Station in Mianxian Country, Hanzhong 724200, China.

Soil microbial metabolism is vital for nutrient cycling and stability of an ecosystem. To elucidate the long-term effects of biochar application on nutrient limitations and carbon use efficiency (CUE) of soil microbial metabolisms, biochars pyrolyzed at 450℃ from trunks and branches of fruit trees under an oxygen-limited condition were mixed with the top Lou soils (0-20 cm) with application amounts of 0, 20, 40, 60, and 80 t·hm in 2012. Corn-wheat rotation was carried out afterwards for seven years. Read More

View Article and Full-Text PDF

Application of the cbh1 promoter in Mortierella alpina and optimization of induction conditions.

Lett Appl Microbiol 2020 Aug 12;71(2):164-170. Epub 2020 May 12.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.

Mortierella alpina has gained remarkable interest due to its high capacity for arachidonic acid (AA) production and potential for eicosapentaenoic acid (EPA) production recently. However, the development of genetically modified strains is limited by lacking inducible promoters, which can express genes conditionally. Here the inducible promoter of cellobiohydrolase (Pcbh1) was utilized in M. Read More

View Article and Full-Text PDF

Engineering of a highly thermostable endoglucanase from the GH7 family of Bipolaris sorokiniana for higher catalytic efficiency.

Appl Microbiol Biotechnol 2020 May 10;104(9):3935-3945. Epub 2020 Mar 10.

Protein Engineering Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, West Bengal, 741246, India.

In a previous study, we reported an alkaliphilic and thermostable endoglucanase (BsGH7-3) of glycoside hydrolase family 7 (GH7) from the hemibiotrophic plant pathogen Bipolaris sorokiniana. However, the catalytic efficiency of the enzyme was lower than for some other endoglucanases of the GH7 family reported in the literature. To engineer a more active enzyme, we identified conserved residues in the substrate-binding tunnel and on the surface of the protein that could play a role in charge-charge interaction and stabilize the structure. Read More

View Article and Full-Text PDF

Feasibility study of on-site solid-state enzyme production by .

Biotechnol Biofuels 2020 26;13:31. Epub 2020 Feb 26.

Fundamental Technology Center, Honda R&D Co., Ltd., 1-4-1 Chuo, Wako-shi, Saitama, 351-0113 Japan.

Background: The development of biorefinery systems that use lignocellulosic biomass as a renewable carbon source to produce fuels and chemicals is attracting increasing attention. The process cost of enzymatic saccharification of biomass is a major challenge for commercialization. To decrease this cost, researchers have proposed on-site solid-state fermentation (SSF). Read More

View Article and Full-Text PDF
February 2020

Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose.

Biotechnol Biofuels 2020 18;13:10. Epub 2020 Jan 18.

1Biological and Agricultural Engineering, University of California, Davis, One Shields Ave., Davis, CA 95616 USA.

Background: Molecular-scale mechanisms of the enzymatic breakdown of cellulosic biomass into fermentable sugars are still poorly understood, with a need for independent measurements of enzyme kinetic parameters. We measured binding times of cellobiohydrolase Cel7A (Cel7A) on celluloses using wild-type Cel7A (WT), the catalytically deficient mutant Cel7A E212Q (E212Q) and their proteolytically isolated catalytic domains (CD) (WT and E212Q, respectively). The binding time distributions were obtained from time-resolved, super-resolution images of fluorescently labeled enzymes on cellulose obtained with total internal reflection fluorescence microscopy. Read More

View Article and Full-Text PDF
January 2020

Influence of the carbohydrate-binding module on the activity of a fungal AA9 lytic polysaccharide monooxygenase on cellulosic substrates.

Biotechnol Biofuels 2019 3;12:206. Epub 2019 Sep 3.

2Biodiversité et Biotechnologie Fongiques, UMR1163, INRA, Aix Marseille Université, Marseille, France.

Background: Cellulose-active lytic polysaccharide monooxygenases (LPMOs) secreted by filamentous fungi play a key role in the degradation of recalcitrant lignocellulosic biomass. They can occur as multidomain proteins fused to a carbohydrate-binding module (CBM). From a biotech perspective, LPMOs are promising innovative tools for producing nanocelluloses and biofuels, but their direct action on cellulosic substrates is not fully understood. Read More

View Article and Full-Text PDF
September 2019

Validation of leaf enzymes in the detergent and textile industries: launching of a new platform technology.

Plant Biotechnol J 2019 06 23;17(6):1167-1182. Epub 2019 Apr 23.

Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Chemical catalysts are being replaced by biocatalysts in almost all industrial applications due to environmental concerns, thereby increasing their demand. Enzymes used in current industries are produced in microbial systems or plant seeds. We report here five newly launched leaf-enzyme products and their validation with 15 commercial microbial-enzyme products, for detergent or textile industries. Read More

View Article and Full-Text PDF

Effects of soil warming on soil microbial extracellular enzyme activities with different depths in a young Chinese fir (Cunninghamia lanceolata)plantation of subtropics.

Ying Yong Sheng Tai Xue Bao 2019 Mar;30(3):832-840

State Key Laboratory for Subtropical Mountain Ecology, Fujian Normal University, Fuzhou 350007, China.

Extracellular enzyme activitie (EEAs) are a sensitive indicator of microbial function and soil organic matter decomposition in response to climate warming. Up to now, most studies of climate warming and their effects on EEAs have been restricted on the relatively carbon rich topsoil (the upper 20 cm of the soil), whereas little is known about EEAs in subsoil (below 30 cm depth). This study focused on the responses of EEAs to soil warming in a subtropical forest at depths of 0-10 cm, 10-20 cm, 20-40 cm and 40-60 cm. Read More

View Article and Full-Text PDF

Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis.

Bioresour Technol 2019 May 10;280:303-312. Epub 2019 Feb 10.

VTT Technical Research Centre of Finland Ltd, P.O Box 1000, 02044 VTT, Finland. Electronic address:

Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Read More

View Article and Full-Text PDF

Benchmarking hydrolytic potential of cellulase cocktail obtained from mutant strain of IIPC 324 with commercial biofuel enzymes.

3 Biotech 2019 Jan 2;9(1):23. Epub 2019 Jan 2.

1Biotechnology Conversion Area, Biofuels Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248005 India.

In the present study, an attempt was made to benchmark the hydrolytic potential of cellulase cocktail obtained from stable mutant UV-8 of IIPC 324 (NFCCI 4117) with three commercially available cellulases. With two experimental approaches, acid-pretreated sugarcane bagasse was subjected to hydrolysis for 72 h, where all the enzymes were dosed on the basis of common protein or common cellulase activity /g cellulose content. Concentrated fungal enzyme (CFE) of mutant UV-8 resulted in ~ 59% and 55% saccharification of acid-pretreated sugarcane bagasse after 72 h at 55 °C and pH 4. Read More

View Article and Full-Text PDF
January 2019

Binding and hydrolysis properties of engineered cellobiohydrolases and endoglucanases.

Bioresour Technol 2018 Nov 19;267:235-241. Epub 2018 Jun 19.

State Key Laboratory of Microbial Technology, Shandong University, No. 72, Binhai Road, Qingdao 266237, PR China. Electronic address:

Because cellulase was the main enzyme used in bioconversion of lignocellulose, it was a valid way to reduce the hydrolysis cost by increasing the adsorption and hydrolysis efficiency of cellulase. In this study, modified cellobiohydrolases (CBHs) and endoglucanases (EGs) were constructed. Two engineered cellulases CBH-TrCBM and EG-TrCBM well-performed during hydrolysis. Read More

View Article and Full-Text PDF
November 2018

Effects of polystyrene nanoparticles on the microbiota and functional diversity of enzymes in soil.

Environ Sci Eur 2018 4;30(1):11. Epub 2018 May 4.

1Depart. Soil Science, Faculty of Regional and Environmental Science, University of Trier, Behringstraße 21, 54296 Trier, Germany.

Background: The increasing production of nanoplastics and the fragmentation of microplastics into smaller particles suggest a plausible yet unclear hazard in the natural environment, such as soil. We investigated the short-term effects (28 days) of polystyrene nanoparticles (PS-NPs) on the activity and biomass of soil microbiota, and the functional diversity of soil enzymes at environmental relevant low levels in an incubation experiment.

Results: Our results showed a significant decrease in microbial biomass in treatments of 100 and 1000 ng PS-NP g DM throughout the incubation period. Read More

View Article and Full-Text PDF

Silver Toxicity Thresholds for Multiple Soil Microbial Biomarkers.

Environ Sci Technol 2018 08 16;52(15):8745-8755. Epub 2018 Jul 16.

Future Industries Institute , University of South Australia , Mawson Lakes 5095 , Australia.

Material flow analysis shows that soil is a key repository for silver (Ag) from (nano)silver-functionalized consumer products, but the potential effects of Ag toxicity, via Ag release, on soil microbial communities and their ecosystem services remains largely unknown. We examined the responses of multiple microbial biomarkers to increasing Ag doses (nine concentrations, 0-2000 mg kg) in nine different soils representing a wide range of soil properties. Analyses included substrate-induced microbial respiration, nine different soil enzyme activities, and quantification of bacterial 16S-rRNA (SSU) and fungal intergenic spacer (ITS) copies. Read More

View Article and Full-Text PDF

Inhibited enzyme activities in soil macroaggregates contribute to enhanced soil carbon sequestration under afforestation in central China.

Sci Total Environ 2018 Nov 2;640-641:653-661. Epub 2018 Jun 2.

Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, PR China. Electronic address:

The fate of soil organic carbon (SOC) sequestered by afforestation is crucial for the mitigation of the anthropogenic climate change but remains largely unclear. This lack of knowledge is particularly true for SOC turnover driven by enzyme activity. Here we measured hydrolase (including β-glucosidase, α-glucosidase, cellobiohydrolase and xylanase) and oxidase (including polyphenol oxidase and peroxidase) activities in soil aggregates following 30-year afforestation in central China. Read More

View Article and Full-Text PDF
November 2018

Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility.

Biotechnol Biofuels 2018 15;11:41. Epub 2018 Feb 15.

1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.

Background: The high cost of enzymes is one of the key technical barriers that must be overcome to realize the economical production of biofuels and biomaterials from biomass. Supplementation of enzyme cocktails with lytic polysaccharide monooxygenase (LPMO) can increase the efficiency of these cellulase mixtures for biomass conversion. The previous studies have revealed that LPMOs cleave polysaccharide chains by oxidization of the C1 and/or C4 carbons of the monomeric units. Read More

View Article and Full-Text PDF
February 2018

Disruption of zinc finger DNA binding domain in catabolite repressor Mig1 increases growth rate, hyphal branching, and cellulase expression in hypercellulolytic fungus NCIM1228.

Biotechnol Biofuels 2018 25;11:15. Epub 2018 Jan 25.

1Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067 India.

Background: There is an urgent requirement for second-generation bio-based industries for economical yet efficient enzymatic cocktail to convert diverse cellulosic biomass into fermentable sugars. In our previous study, secretome of NCIM1228 showed high commercial potential by exhibiting high biomass hydrolyzing efficiency. To develop NCIM1228 further as an industrial workhorse, one of the major genetic interventions needed is global deregulation of cellulolytic genes to achieve higher enzyme production. Read More

View Article and Full-Text PDF
January 2018

Lipopeptide produced from sp. W112 improves the hydrolysis of lignocellulose by specifically reducing non-productive binding of cellulases with and without CBMs.

Biotechnol Biofuels 2017 14;10:301. Epub 2017 Dec 14.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.

Background: Surfactants have attracted increasing interest for their capability to improve the enzymatic hydrolysis of lignocellulosic biomass. Compared to chemical surfactants, biosurfactants have a broader prospect for industrial applications because they are more environmentally friendly and more effective in some researches. Commercial cellulase preparations are mainly composed of endoglucanases (EGs) and cellobiohydrolases (CBHs) that possess carbohydrate-binding modules (CBMs). Read More

View Article and Full-Text PDF
December 2017

Enhancing Cellulase and Hemicellulase Production in Trichoderma orientalis EU7-22 via Knockout of the creA.

Mol Biotechnol 2018 Jan;60(1):55-61

College of Energy, Xiamen University, Xiamen, 361005, China.

The role of the transcription factor creA-mediating carbon catabolite repression in Trichoderma orientalis EU7-22 was investigated for cellulase and hemicellulase production. The binary vector pUR5750G/creA::hph was constructed to knock out creA by homologous integration, generating the ΔcreA mutant Trichoderma orientalis CF1D. For strain CF1D, the filter paper activities (FPA), endoglucanase activities (CMC), cellobiohydrolase activity(CBH), β-glucosidase activity (BG), xylanase activity (XYN), and extracellular protein concentration were 1. Read More

View Article and Full-Text PDF
January 2018

Microbial decomposition is highly sensitive to leaf litter emersion in a permanent temperate stream.

Sci Total Environ 2018 Apr 1;621:486-496. Epub 2017 Dec 1.

Institute of Aquatic Ecology, University of Girona, Campus Montilivi, 17071 Girona, Spain.

Drought frequency and intensity in some temperate regions are forecasted to increase under the ongoing global change, which might expose permanent streams to intermittence and have severe repercussions on stream communities and ecosystem processes. In this study, we investigated the effect of drought duration on microbial decomposition of Populus nigra leaf litter in a temperate permanent stream (Oliveira, NW Portugal). Specifically, we measured the response of the structural (assemblage composition, bacterial and fungal biomass) and functional (leaf litter decomposition, extracellular enzyme activities (EEA), and fungal sporulation) parameters of fungal and bacterial communities on leaf litter exposed to emersion during different time periods (7, 14 and 21d). Read More

View Article and Full-Text PDF

Production of Recombinant Cellobiohydrolase II in a New Expression System Based on .

Enzyme Res 2017 30;2017:6980565. Epub 2017 Aug 30.

Grupo de Biotecnología Ambiental e Industrial (GBAI), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.

Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of cellobiohydrolase II (CBHII) in a native strain of . Read More

View Article and Full-Text PDF

Understanding the role of residues around the active site tunnel towards generating a glucose-tolerant β-glucosidase from Agrobacterium tumefaciens 5A.

Protein Eng Des Sel 2017 07;30(7):523-530

Protein Engineering Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research, Mohanpur 741246, India.

Most β-glucosidases are subjected to inhibition by the final hydrolysis product glucose resulting in the accumulation of cellobiose and oligosaccharides. This accumulated cellobiose and oligosaccharides further inhibit the activities of endoglucanase and cellobiohydrolases, resulting in the inhibition of cellulose degradation and a more expensive biofuel. To elucidate the mechanism(s) of glucose tolerance, we designed and characterised six mutations of a moderately glucose-tolerant β-glucosidase (H0HC94) from the mesophilic bacterium Agrobacterium tumefaciens 5A. Read More

View Article and Full-Text PDF

[Effects of nitrogen additions on soil hydrolase and oxidase activities in Pinus elliottii plantations.]

Ying Yong Sheng Tai Xue Bao 2016 Nov;27(11):3427-3434

Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China.

We evaluated responses of hydrolase and oxidase activities in a subtropical Pinus elliottii plantation through a nitrogen (N) addition field experiment (dosage level: 0, 40, 120 kg N·hm·a). The results showed that N additions significantly decreased the carbon, nitrogen and phosphorus related hydrolase and oxidase activities. The activities of β-1,4-glucosidase (BG), cellobiohydrolase (CBH), β-1,4-N-acetylglucosaminidase (NAG) and peroxidase (PER) activities were decreased by 16. Read More

View Article and Full-Text PDF
November 2016

Allyl Isothiocyanate Inhibits the Proliferation of Renal Carcinoma Cell Line GRC-1 by Inducing an Imbalance Between Bcl2 and Bax.

Med Sci Monit 2016 Nov 10;22:4283-4288. Epub 2016 Nov 10.

Department of Clinical Laboratory, Chengdu Military General Hospital, Chengdu, Sichuan, China (mainland).

BACKGROUND Because of the insensitivity of renal cell carcinoma (RCC) to both chemotherapy and radiotherapy, surgery remains the primary approach for anticancer treatment. However, patients who do not receive timely diagnoses may not be suitable for surgery, especially in the late phase of tumor development. Thus, the discovery of novel effective treatment is of great importance. Read More

View Article and Full-Text PDF
November 2016