275,001 results match your criteria cell-matrix

Fabrication of Hyaluronic Acid-Gold Nanoparticles with Chitosan to Modulate Neural Differentiation of Mesenchymal Stem Cells.

J Chin Med Assoc 2021 Jul 26. Epub 2021 Jul 26.

Neurological Institute Head of Department of Neurosurgery Taichung Veterans General Hospital, Taichung, Taiwan, ROC Department of Physical Therapy, Hung Kuang University, Taichung, Taiwan, ROC Basic Medical Education Center, Central Taiwan University of Science and Technology, Taichung, Taiwan, ROC Graduate Institute of Biomedical Science, China Medical University, Taichung, Taiwan, ROC Department of Occupational Safety and Health, China Medical University, Taichung, Taiwan, ROC Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC Blood Bank, Taichung Veterans General Hospital, Taichung, Taiwan, ROC Translational Medicine Research, China Medical University Hospital, Taichung, Taiwan, ROC.

Background: Chitosan (Chi) is a natural material which has been widely used in neural applications due to possessing better biocompatibility. In this research study, a novel of nanocomposites film based on Chitosan (Chi) with Hyaluronic Acid (HA), combined with varying amounts of gold nanoparticles (AuNPs) was created resulting in pure Chi, Chi-HA, Chi-HA-AuNPs (25 ppm) and Chi-HA-AuNPs (50 ppm).

Methods: This study focused on evaluating their effects on Mesenchymal Stem Cell (MSC) viability, colony formation and biocompatibility. Read More

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The E-Modulus of the Oocyte Is a Non-Destructive Measure of Zona Pellucida Hardening.

Reproduction 2021 Jul 1. Epub 2021 Jul 1.

J Floehr, Helmholtz-Insitute for Biomedical Engineering, Bioninterface Laboratory, RWTH Aachen University Medical Faculty, Aachen, Germany.

After fertilization, the oocyte-specific metalloproteinase ovastacin is released and cleaves the zona pellucida protein 2 (ZP2), making the zona pellucida impermeable to sperm. Before fertilization, the zona remains permeable because previously released ovastacin is inhibited by fetuin-B. Consequently, in the absence of fetuin-B, ZP2 cleavage occurs prematurely and leads to infertility of female fetuin-B deficient mice. Read More

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Spinal Ninjurin2 contributes to the neuropathic pain via NF-κB-mediated neuroinflammation in the spared sciatic nerve injury rats.

Int Immunopharmacol 2021 Jul 25;99:107918. Epub 2021 Jul 25.

Department of Pain Management, the First Affiliated Hospital of Zhengzhou University, 450052 Zhengzhou, China. Electronic address:

Object: Ninjurin2 (nerve injury induced protein 2, NINJ2) is a molecule which mediates cell-to-cell and cell-to-extracellular matrix interactions in the nervous system. Clinical study shows NINJ2 is associated with the development of postherpetic neuralgia. However, it is lack of direct evidence that NINJ2 participated in neuropathic pain. Read More

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Nascent RNA scaffolds contribute to chromosome territory architecture and counter chromatin compaction.

Mol Cell 2021 Jul 20. Epub 2021 Jul 20.

Department of Neurology and Pediatrics, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA. Electronic address:

Nuclear chromosomes transcribe far more RNA than required to encode protein. Here we investigate whether non-coding RNA broadly contributes to cytological-scale chromosome territory architecture. We develop a procedure that depletes soluble proteins, chromatin, and most nuclear RNA from the nucleus but does not delocalize XIST, a known architectural RNA, from an insoluble chromosome "scaffold. Read More

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Complete representation of action space and value in all dorsal striatal pathways.

Cell Rep 2021 Jul;36(4):109437

Department of Neuroscience, Karolinska Institutet, 171 77 Stockholm, Sweden. Electronic address:

The dorsal striatum plays a central role in the selection, execution, and evaluation of actions. An emerging model attributes action selection to the matrix and evaluation to the striosome compartment. Here, we use large-scale cell-type-specific calcium imaging to determine the activity of striatal projection neurons (SPNs) during motor and decision behaviors in the three major outputs of the dorsomedial striatum: Oprm1 striosome versus D1 direct and A2A indirect pathway SPNs. Read More

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Kidney stone proteomics: An update and perspectives.

Expert Rev Proteomics 2021 Jul 28. Epub 2021 Jul 28.

Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Introduction: Main problems of kidney stone disease are its increasing prevalence and high recurrence rate after calculi removal in almost all areas around the globe. Despite enormous efforts in the past, its pathogenic mechanisms remain unclear and need further elucidations. Proteomics has thus become an essential tool to unravel such sophisticated disease mechanisms at cellular, subcellular, molecular, tissue and whole organism levels. Read More

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Deep Penetration of Nanolevel Drugs and Micrometer-Level T Cells Promoted by Nanomotors for Cancer Immunochemotherapy.

J Am Chem Soc 2021 Jul 28. Epub 2021 Jul 28.

The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing University, Nanjing 210008, China.

The ability of nanomotors to promote the deep penetration of themselves and the loaded drugs in diseased tissues has been proposed and confirmed. However, whether such motion behavior of the nanomotors can also promote deep penetration of micrometer-sized immune cells in the diseased microenvironment, which is important for the immunotherapy of some diseases, has not been mentioned. Herein, we construct a nitric oxide (NO)-driven nanomotor that can move in the tumor microenvironment, focusing on its motion behavior and the role of NO, the beneficial product released during movement from this kind of nanomotor, in regulating the infiltration behavior and activity of immune cells. Read More

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4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis.

Prostate 2021 Jul 28. Epub 2021 Jul 28.

Department of Urology, The First Affiliated Hospital of Anhui Medical University, Institute of Urology, and Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China.

Background: Hyaluronan (HA), an extracellular matrix component, accumulates in most chronic inflammatory tissues. Here, we studied the impact of HA on the pathogenesis of chronic prostatitis.

Materials And Methods: First, we sorted demographic characteristics and peripheral blood serum samples from patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) to assess the relationship between the levels of HA in peripheral blood serum and the severity of inflammation in patients. Read More

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Facile Synthesis of Metal Nanoparticle-Loaded Polymer Nanocomposite with Highly Efficient an Optically Enhanced Biocidal and Anticancer agents.

J Biomater Sci Polym Ed 2021 Jul 28:1-15. Epub 2021 Jul 28.

School of Chemical Engineering, Yeungnam University, Gyeongsan, Gyeongbuk-do 38541, Republic of Korea.

In this work, we prepared silver nanoparticles (AgNPs)-loaded poly(2,5-dimethoxyaniline;PDMA) nanocomposite via a simple chemical oxidative method to develop new effective biocidal and anticancer agents systems. In situ UV-vis and fluorescence spectroscopy has been used to monitor the formation of PDMA/Ag is strongly dependent on the concentration of PDMA in the present system. The FTIR and XRD studies exhibit that the rate of polymer formation and oxidation state of PDMA/Ag nanocomposite, which confirmed the AgNPs is strongly binding with the PDMA matrix. Read More

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A Large-Scalable, Surfactant-Free, and Ultrastable Ru-Doped PtCo Oxygen Reduction Catalyst.

Nano Lett 2021 Jul 28. Epub 2021 Jul 28.

State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Developing a large-scale method to produce platinum (Pt)-based electrocatalysts for the oxygen reduction reaction (ORR) is highly desirable to propel the commercialization of the membrane electrode assembly (MEA). Here, we successfully report the large-scale production of surfactant-free ruthenium-doped Pt-cobalt octahedra grown on carbon (Ru-PtCo/C), which display a much higher ORR activity and stability and MEA stability than PtCo/C and Pt/C. Significantly, the in-situ X-ray absorption fine structure result reveals that Ru can drive the reduced Pt atoms to reverse to their initial state after the ORR by transferring a redundant electron from Pt to Ru, preventing the over-reduction of Pt active sites and boosting the chemical stability. Read More

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Blocking MMP-12-modulated epithelial-mesenchymal transition by repurposing penfluridol restrains lung adenocarcinoma metastasis via uPA/uPAR/TGF-β/Akt pathway.

Cell Oncol (Dordr) 2021 Jul 28. Epub 2021 Jul 28.

Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 250 Wu-Hsing Street, 11031, Taipei, Taiwan.

Purpose: Metastasis of lung adenocarcinoma (LADC) is a crucial factor determining patient survival. Repurposing of the antipsychotic agent penfluridol has been found to be effective in the inhibition of growth of various cancers. As yet, however, the anti-metastatic effect of penfluridol on LADC has rarely been investigated. Read More

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MicroRNA-486 promotes a more catabolic phenotype in chondrocyte-like cells by targeting SIRT6 : possible involvement in cartilage degradation in osteoarthritis.

Bone Joint Res 2021 Jul;10(7):459-466

Department of Hand Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, China.

Aims: Osteoarthritis (OA) is characterized by persistent destruction of articular cartilage. It has been found that microRNAs (miRNAs) are closely related to the occurrence and development of OA. The purpose of the present study was to investigate the mechanism of miR-486 in the development and progression of OA. Read More

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Paramyxovirus replication induces the Hexosamine Biosynthetic Pathway and Mesenchymal Transition via the IRE1a-XBP1s arm of the Unfolded Protein Response.

Am J Physiol Lung Cell Mol Physiol 2021 Jul 28. Epub 2021 Jul 28.

Department of Internal Medicine, grid.14003.36University of Wisconsin-Madison, Madison, WI, United States.

The paramyoxviridae, Respiratory Syncytial Virus (RSV) and murine respirovirus are enveloped, negative-sense RNA viruses that are the etiological agents of vertebrate respiratory tract infections (LRTIs). We observe RSV infection in human small airway epithelial cells induces accumulation of glycosylated proteins within the ER, increased Glutamine-Fructose-6-Phosphate Transaminases (GFPT1/2), and accumulation of UDP-N-acetylglucosamine, indicating activation of the hexosamine biosynthetic pathway (HBP). RSV infection induces rapid formation of spliced X-box binding protein 1 (XBP1s) and processing of activating transcription factor 6 (ATF6). Read More

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From pulmonary fibrosis to progressive pulmonary fibrosis - a lethal pathobiological jump.

Am J Physiol Lung Cell Mol Physiol 2021 Jul 28. Epub 2021 Jul 28.

Facultad de Ciencias, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico.

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Rapid postmortem ventilation improves donor lung viability by extending the tolerable warm ischemic time after cardiac death in mice.

Am J Physiol Lung Cell Mol Physiol 2021 Jul 28. Epub 2021 Jul 28.

Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, grid.21925.3dUniversity of Pittsburgh, Pittsburgh, PA, United States.

Uncontrolled donation after cardiac death (uDCD) contributes little to ameliorating donor lung shortage due to rapidly progressive warm ischemia after circulatory arrest. Here, we demonstrated non-hypoxia improves donor lung viability in a novel uDCD lung transplant model undergoing rapid ventilation after cardiac death and compared the evolution of ischemia-reperfusion injury in mice that underwent pulmonary artery ligation (PAL). The tolerable warm ischemia time at 37ºC was initially determined in mice using a modified PAL model. Read More

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Protonation-Deprotonation Switch Controls the Amyloid-like Misfolding of Nucleic-Acid-Binding Domains of TDP-43.

J Phys Chem B 2021 Jul 28. Epub 2021 Jul 28.

Physical and Materials Chemistry Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India.

Nutrient starvation stress acidifies the cytosol and leads to the formation of large protein assemblies and misfolded aggregates. However, how starvation stress is sensed at the molecular level and leads to protein misfolding is poorly understood. TDP-43 is a vital protein, which, under stress-like conditions, associates with stress granule proteins via its functional nucleic-acid-binding domains (TDP-43) and misfolds to form aberrant aggregates. Read More

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Upgrading Hepatic Differentiation and Functions on 3D Printed Silk-Decellularized Liver Hybrid Scaffolds.

ACS Biomater Sci Eng 2021 Jul 28. Epub 2021 Jul 28.

Regenerative Engineering Laboratory, Department of Textile and Fiber Technology, Indian Institute of Technology-Delhi, New Delhi 110016, India.

We developed hybrid liver-specific three-dimensional (3D) printed scaffolds using a solubilized native decellularized liver (DCL) matrix and silk fibroin (SF) and investigated their ability to support functional cultures of hepatic cells. Rat livers were decellularized by perfusing detergents via the portal vein, solubilized using pepsin to form DCL, and characterized. SF blended with gelatin (8% w/v) was optimized with varying percentages of DCL to obtain silk gelatin-DCL bioink (SG-DCL). Read More

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Antibody-Free Mass Spectrometry Identification of Vascular Integrity Markers in Major Trauma.

Neurotrauma Rep 2021 1;2(1):322-329. Epub 2021 Jul 1.

Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Richland, Washington, USA.

Antibody mediated strategies for protein biomarker detection are common, but may limit discovery. We hypothesized that the use of antibody-free proteomics is feasible for detecting protein biomarkers in plasma of patients sustaining major trauma. A subset of subjects with major trauma from a prospective observational trial were analyzed. Read More

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Pathological Q-Waves With Coronary Artery Spasm.

JACC Case Rep 2021 Apr 10;3(4):555-560. Epub 2021 Feb 10.

Department of Cardiology and Nephrology, Mie University Graduate School of Medicine, Tsu, Mie, Japan.

This case highlights the diagnostic challenge associated with myocarditis, especially when accompanied by coronary spasm. Any coronary spasm with hemodynamic instability and/or an inexplicable widespread electrocardiogram should alert the clinician to the possibility of fulminant myocarditis and the necessity of endomyocardial biopsy for treatment decisions. (). Read More

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Cyclic Stiffness Modulation of Cell-Laden Protein-Polymer Hydrogels in Response to User-Specified Stimuli including Light.

Adv Biosyst 2018 Dec 12;2(12). Epub 2018 Oct 12.

Department of Chemical Engineering, University of Washington, 3781 Okanogan Lane NE, Seattle, WA, 98195, USA; Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA, 98105, USA; Institute of Stem Cell & Regenerative Medicine, University of Washington, 850 Republican St., Seattle, WA, 98109, USA; Molecular Engineering & Sciences Institute, University of Washington, 3946 W Stevens Way NE, Seattle, WA, 98195, USA.

Although mechanical signals presented by the extracellular matrix are known to regulate many essential cell functions, the specific effects of these interactions, particularly in response to dynamic and heterogeneous cues, remain largely unknown. Here, we introduce a modular semisynthetic approach to create protein-polymer hydrogel biomaterials that undergo reversible stiffening in response to user-specified inputs. Employing a novel dual-chemoenzymatic modification strategy, we create fusion protein-based gel crosslinkers that exhibit stimuli-dependent intramolecular association. Read More

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December 2018

Glucocorticoid and mineralocorticoid receptor expression in critical illness: A narrative review.

World J Crit Care Med 2021 Jul 9;10(4):102-111. Epub 2021 Jul 9.

1 Department of Critical Care Medicine & Pulmonary Services, School of Medicine, National & Kapodistrian University of Athens, "Evangelismos" Hospital, Athens 10676, Greece.

The glucocorticoid receptor (GCR) and the mineralocorticoid receptor (MR) are members of the steroid receptor superfamily of hormone-dependent transcription factors. The receptors are structurally and functionally related. They are localized in the cytosol and translocate into the nucleus after ligand binding. Read More

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Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional.

Nat Commun 2021 07 27;12(1):4566. Epub 2021 Jul 27.

Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.

The airway epithelium serves as the interface between the host and external environment. In many chronic lung diseases, the airway is the site of substantial remodeling after injury. While, idiopathic pulmonary fibrosis (IPF) has traditionally been considered a disease of the alveolus and lung matrix, the dominant environmental (cigarette smoking) and genetic (gain of function MUC5B promoter variant) risk factor primarily affect the distal airway epithelium. Read More

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Short stature and combined immunodeficiency associated with mutations in RGS10.

Sci Signal 2021 Jul 27;14(693). Epub 2021 Jul 27.

Lung and Vascular Inflammation Section, Laboratory of Allergic Diseases, NIAID/NIH Bethesda, MD 20892, USA.

We report the clinical and molecular phenotype of three siblings from one family, who presented with short stature and immunodeficiency and carried uncharacterized variants in (c.489_491del:p.E163del and c. Read More

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Anti-glomerular basement membrane disease and IgA nephropathy in a patient with previous renal cell carcinoma.

BMJ Case Rep 2021 Jul 27;14(7). Epub 2021 Jul 27.

Department of Anatomical Pathology, Royal North Shore Hospital, Sydney, New South Wales, Australia.

A 66-year-old Asian woman presented with severe kidney injury, microscopic haematuria and subnephrotic range proteinuria with elevated serum anti-glomerular basement membrane (anti-GBM) titre. She had a history of renal cell carcinoma. Renal biopsy revealed dual pathology with immunofluorescence showing 3+ linear glomerular IgG staining and 3+ IgA mesangial staining. Read More

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Sublethal doxorubicin promotes migration and invasion of breast cancer cells: role of Src Family non-receptor tyrosine kinases.

Breast Cancer Res 2021 Jul 27;23(1):76. Epub 2021 Jul 27.

Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794- 8430, USA.

Background: Doxorubicin (Dox) is a widely used chemotherapy, but its effectiveness is limited by dose-dependent side effects. Although lower Dox doses reduce this risk, studies have reported higher recurrence of local disease with no improvement in survival rate in patients receiving low doses of Dox. To effectively mitigate this, a better understanding of the adverse effects of suboptimal Dox doses is needed. Read More

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Chitosan-Calcium-Simvastatin Scaffold as an Inductive Cell-Free Platform.

J Dent Res 2021 Jul 27:220345211024207. Epub 2021 Jul 27.

Department of Physiology and Pathology, University of Estadual Paulista-UNESP, Araraquara School of Dentistry, Araraquara, SP, Brazil.

The development of biomaterials based on the combination of biopolymers with bioactive compounds to develop delivery systems capable of modulating dentin regeneration mediated by resident cells is the goal of current biology-based strategies for regenerative dentistry. In this article, the bioactive potential of a simvastatin (SV)-releasing chitosan-calcium-hydroxide (CH-Ca) scaffold was assessed. After the incorporation of SV into CH-Ca, characterization of the scaffold was performed. Read More

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Nidogen-2 Maintains the Contractile Phenotype of Vascular Smooth Muscle Cells and Prevents Neointima Formation via Bridging Jagged1-Notch3 Signaling.

Circulation 2021 Jul 28. Epub 2021 Jul 28.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China; Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China.

How the extracellular matrix (ECM) microenvironment modulates the contractile phenotype of vascular smooth muscle cells (VSMCs) and confers vascular homeostasis remains elusive. To explore the key ECM proteins in the maintenance of the contractile phenotype of VSMCs, we applied protein-protein interaction (PPI) network analysis to explore novel ECM proteins associated with the VSMC phenotype. By combining and genetic mice vascular injury model, we identified nidogen-2, a basement membrane (BM) glycoprotein, as a key ECM protein for maintenance of vascular smooth muscle cell identity. Read More

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Deregulated glutamate to pro-collagen conversion is associated with adverse outcome in lung cancer and may be targeted by renin-angiotensin-aldosterone system (RAS) inhibition.

Lung Cancer 2021 Jul 16;159:84-95. Epub 2021 Jul 16.

Department of Internal Medicine V (Haematology & Oncology), Medical University of Innsbruck, Innsbruck, Austria. Electronic address:

Background: The tumor-microenvironment (TME) represents an attractive therapeutic target in NSCLC and plays an important role for efficacy of cancer therapeutics. We hypothesized that upregulation of collagen synthesis might be associated with adverse outcome in NSCLC. Literature evidence suggests that renin-angiotensin system inhibitors (RASi) decrease collagen deposition. Read More

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Arabidopsis HIPP proteins regulate endoplasmic reticulum-associated degradation of CKX proteins and cytokinin responses.

Mol Plant 2021 Jul 24. Epub 2021 Jul 24.

Institute of Biology, University of Graz, Schubertstraße 51, 8010 Graz, Austria. Electronic address:

Eukaryotic organisms are equipped with quality-control mechanisms that survey protein folding in the endoplasmic reticulum (ER) and remove non-native proteins by ER-associated degradation (ERAD). Recent research has shown that cytokinin-degrading CKX proteins are subjected to ERAD during plant development. The mechanisms of plant ERAD, including the export of substrate proteins from the ER, are not fully understood and the molecular components involved in the ERAD of CKX are unknown. Read More

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Type V Collagen Regulates the Structure and Biomechanics of TMJ Condylar Cartilage: A Fibrous-Hyaline Hybrid.

Matrix Biol 2021 Jul 24. Epub 2021 Jul 24.

School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, United States. Electronic address:

This study queried the role of type V collagen in the post-natal growth of temporomandibular joint (TMJ) condylar cartilage, a hybrid tissue with a fibrocartilage layer covering a secondary hyaline cartilage layer. Integrating outcomes from histology, immunofluorescence imaging, electron microscopy and atomic force microscopy-based nanomechanical tests, we elucidated the impact of type V collagen reduction on TMJ condylar cartilage growth in the type V collagen haploinsufficiency and inducible knockout mice. Reduction of type V collagen led to significantly thickened collagen fibrils, decreased tissue modulus, reduced cell density and aberrant cell clustering in both the fibrous and hyaline layers. Read More

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