680,441 results match your criteria cell revealed

Cyclophilin A regulates secretion of tumour-derived extracellular vesicles.

Transl Oncol 2021 May 10;14(8):101112. Epub 2021 May 10.

UCD School of Biomolecular & Biomedical Science, Conway Institute, University College Dublin (UCD), Belfield, Dublin 4, Ireland. Electronic address:

Extracellular Vesicles (EVs) are a heterogenous population of particles that play an important role in cell-cell communication in physiological and pathophysiological situations. In this study we reveal that the peptidyl prolyl isomerase Cyclophilin A (CypA) is enriched in cancer-derived EVs from a range of haematopoietic malignancies. CypA-enriched blood cancer EVs were taken up by normal monocytes independent of EV surface trypsin-sensitive proteins and potently stimulated pro-inflammatory MMP9 and IL-6 secretion. Read More

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Host factor cyclophilin B affects Orf virus replication by interacting with viral ORF058 protein.

Vet Microbiol 2021 May 4;258:109099. Epub 2021 May 4.

Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:

Poxviruses have evolved multiple strategies to modulate host-derived factors to create an optimal environment for viral efficient replication. Our previous study indicated that cyclophilin B (CypB) is a critical factor for ORFV replication in MDBK cells. However, the precise molecular mechanism by which CypB facilitates ORFV replication remains less understood. Read More

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Treatment of fish market wastewater and energy production using halophiles in air cathode microbial fuel cell.

J Environ Manage 2021 May 10;292:112752. Epub 2021 May 10.

Department of Marine Biology, Faculty of Marine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Center of Excellence in Environmental Studies, King Abdulaziz University, Jeddah, 21589, Saudi Arabia. Electronic address:

The present study is aimed to treat the fish market wastewater coupled with electricity production using halophiles in microbial fuel cell (MFC) technology under saline condition (4.6%). Halophilic consortium obtained from desalination plant brine water was used in the lab scale air cathode microbial fuel cell (ACMFC) reactor equipped with carbon brush and carbon cloth as anode and cathode. Read More

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Interactions of core cross-linked poly(2-oxazoline) and poly(2-oxazine) micelles with immune cells in human blood.

Biomaterials 2021 May 4;274:120843. Epub 2021 May 4.

ARC Centre of Excellence in Convergent Bio-Nano Science & Technology, and Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, 3052, Australia; Materials Science and Engineering, Monash University, Clayton, VIC, 3800, Australia. Electronic address:

Water-soluble poly(cyclic imino ether)s (PCIEs) have emerged as promising biocompatible polymers for nanomedicine applications in recent years. Despite their generally accepted stealth properties, there has been no comprehensive evaluation of their interactions with primary immune cells in human blood. Here we present a library of core cross-linked micelles (CCMs) containing various PCIE shells. Read More

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Quaternary ammonium iminofullerenes promote root growth and osmotic-stress tolerance in maize via ROS neutralization and improved energy status.

Plant Physiol Biochem 2021 May 1;164:122-131. Epub 2021 May 1.

NanoAgro Center, College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address:

In the present study, the role of quaternary ammonium iminofullerenes (IFQA) on the root growth of plant seedlings was investigated. The root elongation of Arabidopsis and maize exposed to 20 and 50 mg/L of IFQA was promoted under normal and osmotic stress conditions, respectively. In the meantime, the root active absorption area and adenosine triphosphate content in roots of maize seedlings were enhanced by IFQA treatment, however, the contents of hydrogen peroxide (HO) and malondialdehyde in roots were down-regulated. Read More

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Cadmium disturbs epigenetic modification and induces DNA damage in mouse preimplantation embryos.

Ecotoxicol Environ Saf 2021 May 11;219:112306. Epub 2021 May 11.

College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, PR China. Electronic address:

Cadmium is an environmental pollutant that has extensive deleterious effects on the reproductive system. However, the mechanisms underlying the effects of cadmium on preimplantation embryos are unclear. Here, we used a mouse model to investigate the effects of maternal cadmium (32 mg/l) exposure in drinking water for 2 days on early embryonic development, and studied the mechanisms associated with epigenetic modifications and DNA damage induced by oxidative stress. Read More

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Expression pattern of transcriptional enhanced associate domain family member 1 (Tead1) in developing mouse molar tooth.

Gene Expr Patterns 2021 May 10:119182. Epub 2021 May 10.

Department of Maxillofacial Orthognathics, Division of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.

The Hippo pathway is essential for determining organ size by regulating cell proliferation. Previous reports have shown that impairing this pathway causes abnormal tooth development. However, the precise expression profile of the members of the transcriptional enhanced associate domain family (Tead), which are key transcription factors mediating Yap function, during tooth development is unclear. Read More

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First report of fatal baylisascariasis-induced acute pancreatitis in a giant panda.

Parasitol Int 2021 May 10:102380. Epub 2021 May 10.

Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Sich3uan Academy of Giant Panda, Chengdu, Sichuan, China.

A wild adult male giant panda that was rescued from a nature reserve in Sichuan Province, China, has died. The panda had been in poor physical condition: it was wheezing and had increased serum amylase. A pathological examination was performed in order to determine the cause of death. Read More

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Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC) -derived sensory neurons.

Neurobiol Dis 2021 May 10:105391. Epub 2021 May 10.

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Klinik und Hochschulambulanz für Neurologie, Charitéplatz 1, 10117 Berlin, Germany; Berlin Institute of Health at Charité, Universitätsmedizin Berlin, Anna-Louisa-Karsch Straße 2, 10178 Berlin, Germany; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, NeuroCure Cluster of Excellence, Charitéplatz 1, 10117 Berlin, Germany; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Center for Stroke Research Berlin, Charitéplatz 1, 10117 Berlin, Germany; German Center for Neurodegenerative Diseases (DZNE), partner site Berlin, Germany; German Center for Cardiovascular Research (DZHK), partner site Berlin, Germany.

Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent, potentially irreversible adverse effect of cytotoxic chemotherapy often leading to a reduction or discontinuation of treatment which negatively impacts patients' prognosis. To date, however, neither predictive biomarkers nor preventive treatments for CIPN are available, which is partially due to a lack of suitable experimental models. We therefore aimed to evaluate whether sensory neurons derived from induced pluripotent stem cells (iPSC-DSN) can serve as human disease model system for CIPN. Read More

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Dopamine neuron-specific LRRK2 G2019S effects on gene expression revealed by translatome profiling.

Neurobiol Dis 2021 May 10:105390. Epub 2021 May 10.

Jungers Center for Neurosciences, Department of Neurology, Oregon Health and Science University, Portland, OR, USA. Electronic address:

Leucine-rich repeat kinase 2 (LRRK2) mutations are the most common genetic cause of late-onset autosomal dominant Parkinson's disease. The pathogenic G2019S mutation enhances LRRK2 kinase activity and induces neurodegeneration in C. elegans, Drosophila and rodent models through unclear mechanisms. Read More

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Promising anti-SARS-CoV-2 drugs by effective dual targeting against the viral and host proteases.

Bioorg Med Chem Lett 2021 May 10:128099. Epub 2021 May 10.

Research Institute for Medical and Health sciences, University of Sharjah, P.O. Box 27272, Sharjah, UAE; College of Pharmacy, University of Sharjah, P.O. Box 27272, Sharjah, UAE. Electronic address:

SARS-CoV-2 caused dramatic health, social and economic threats to the globe. With this threat, the expectation of future outbreak, and the shortage of anti-viral drugs, scientists were challenged to develop novel antivirals. The objective of this study is to develop novel anti-SARS-CoV-2 compounds with dual activity by targeting valuable less-mutated enzymes. Read More

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Chinese herbal medicine Feiyanning cooperates with cisplatin to enhance cytotoxicity to non-small-cell lung cancer by inhibiting protective autophagy.

J Ethnopharmacol 2021 May 10:114196. Epub 2021 May 10.

Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, PR China. Electronic address:

Ethnopharmacological Relevance: Feiyanning (FYN), the Chinese herbal medicine (CHM), has been used to manage non-small cell lung cancer (NSCLC) for the past 23 years. Chemotherapeutic drugs can induce autophagy in cancer cells to protect themselves from death. However, FYN can inhibit the protective autophagy in cancer cells. Read More

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Cellular senescence and the senescence-associated secretory phenotype: Potential therapeutic targets for renal fibrosis.

Exp Gerontol 2021 May 10:111403. Epub 2021 May 10.

School of Medicine, Nankai University, Tianjin 300071, China; Department of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Chinese PLA Institute of Nephrology, Beijing Key Laboratory of Kidney Diseases, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China. Electronic address:

Renal fibrosis plays a crucial role in the progression of chronic kidney disease and end-stage renal disease. However, because the aetiology of this pathological process is complex and remains unclear, there is still no effective treatment. Cellular senescence and the senescence-associated secretory phenotype (SASP) have been reported to lead to renal fibrosis. Read More

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Establishment of lncRNA-mRNA network in bovine oocyte between germinal vesicle and metaphase II stage.

Gene 2021 May 10:145716. Epub 2021 May 10.

College of Life Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China. Electronic address:

Long non-coding RNA (lncRNA), a type of non-protein coding transcripts with lengths exceeding 200 nucleotides, is reported to be widely involved in many cellular and developmental processes. However, few roles of lncRNA in oocyte development have been defined. In this study, to uncover the effect of lncRNA during oocyte maturation, bovine germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes underwent RNA sequencing. Read More

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High-throughput fabrication of silk fibroin/hydroxypropyl methylcellulose (SF/HPMC) manofibrous scaffolds for skin tissue engineering.

Int J Biol Macromol 2021 May 10. Epub 2021 May 10.

National Engineering Laboratory for Modern Silk, College of Textile and Engineering, Soochow University, 199 Ren-ai Road, Suzhou 215123, China.

Silk fibroin (SF) is a natural macromolecule material with good biocompatibility, which can be used to prepare a variety of biological materials. In this study, hydroxypropyl methylcellulose (HPMC) was applied to improve the properties of SF nanofibrous scaffolds (NFS) for skin tissue engineering applications. SF/HPMC NFS with varying weight ratios of SF: HPMC were prepared in batches by a modified free surface electrospinning. Read More

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Fabrication of dual layered biocompatible herbal biopatch from biological waste for skin - Tissue regenerative applications.

Int J Biol Macromol 2021 May 10. Epub 2021 May 10.

Department of Biomedical Engineering, K.P.R. Institute of Engineering and Technology, Coimbatore 641048, India.

A dual layered herbal biopolymeric patch (biopatch) with enhanced wound healing efficiency and skin mimicking functions was fabricated for skin-tissue regenerative applications. In this study, hoof keratin (KE) extracted from biological waste and gelatin (GE) was employed for KE-GE biosheet fabrication using a simple casting method. Further, the top layer of the fabricated KE-GE biosheet was coated with bioactive Matricaria recutita (Chamomile flower) extract (CH) with gelatin through an electrospraying method. Read More

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Unlocking the PIP-box: A peptide library reveals interactions that drive high affinity binding to human PCNA.

J Biol Chem 2021 May 10:100773. Epub 2021 May 10.

Institute of Photonics and Advanced Sensing, School of Biological Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia. Electronic address:

The human sliding clamp Proliferating Cell Nuclear Antigen (hPCNA) interacts with over 200 proteins through a conserved binding motif, the PIP-box, to orchestrate DNA replication and repair. It is not clear how changes to the features of a PIP-box modulate protein binding and thus how they fine tune downstream processes. Here we present a systematic study of each position within the PIP-box to reveal how hPCNA-interacting peptides bind with drastically varied affinities. Read More

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Two newly identified Haematococcus strains efficiently accumulated radioactive cesium over higher astaxanthin production.

Environ Res 2021 May 10:111301. Epub 2021 May 10.

Department of Biomedical Engineering, Sogang University, Baekbeom-ro 35, Mapo-gu, Seoul, Republic of Korea; Department of Mechanical Engineering, Sogang University, Baekbeom-ro 35, Mapo-gu, Seoul, Republic of Korea. Electronic address:

In this study, we investigated the morphological, genomic and bioaccumulation characteristics of two isolated Haematococcus strains (namely Goyang and Sogang), which were newly discovered in South Korea. Morphological analysis revealed that the isolated strains were unicellular and bi-flagellated green microalgae that formed thickened walls at the palmelloid or red-cyst phase. Phylogenetic analysis of 18S rRNA and rbcL gDNA sequences demonstrated that both strains were taxonomically related to the genus Haematococcus. Read More

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Kinetics and correlates of the neutralizing antibody response to SARS-CoV-2 infection in humans.

Cell Host Microbe 2021 May 3. Epub 2021 May 3.

Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany; German Center for Infection Research, Partner Site Bonn-Cologne, 50931 Cologne, Germany; Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany. Electronic address:

Understanding antibody-based SARS-CoV-2 immunity is critical for overcoming the COVID-19 pandemic and informing vaccination strategies. We evaluated SARS-CoV-2 antibody dynamics over 10 months in 963 individuals who predominantly experienced mild COVID-19. Investigating 2,146 samples, we initially detected SARS-CoV-2 antibodies in 94. Read More

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Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing.

Cell Stem Cell 2021 May 8. Epub 2021 May 8.

Department of Genetics & Developmental Biology, The Rappaport Faculty of Medicine & Research Institute, Technion Integrated Cancer Center, Technion - Israel Institute of Technology, Haifa, Israel. Electronic address:

The accessibility and transparency of the cornea permit robust stem cell labeling and in vivo cell fate mapping. Limbal epithelial stem cells (LSCs) that renew the cornea are traditionally viewed as rare, slow-cycling cells that follow deterministic rules dictating their self-renewal or differentiation. Here, we combined single-cell RNA sequencing and advanced quantitative lineage tracing for in-depth analysis of the murine limbal epithelium. Read More

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Effects of Cholesterol on the Partitioning of a Drug Molecule in Lipid Bilayers.

J Phys Chem B 2021 May 13. Epub 2021 May 13.

Department of Chemistry and Department of Physics, University of Illinois at Chicago, Chicago, Illinois 60607, United States.

Drug molecules either bind to membrane-bound targets or permeate through cell membranes to reach intracellular targets, and hence, their membrane partition and permeation are of great importance. Here, we studied the effects of cholesterol on the partition of amantadine, an antiflu drug molecule, into 1,2-dimyristoyl--glycero-3-phosphocholine bilayers using molecular dynamics simulations. The membrane partition of amantadine is sensitive to the cholesterol mole fraction (). Read More

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Multivariate meta-analysis reveals global transcriptomic signatures underlying distinct human naive-like pluripotent states.

PLoS One 2021 13;16(5):e0251461. Epub 2021 May 13.

NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America.

The ground or naive pluripotent state of human pluripotent stem cells (hPSCs), which was initially established in mouse embryonic stem cells (mESCs), is an emerging and tentative concept. To verify this vital concept in hPSCs, we performed a multivariate meta-analysis of major hPSC datasets via the combined analytic powers of percentile normalization, principal component analysis (PCA), t-distributed stochastic neighbor embedding (t-SNE), and SC3 consensus clustering. This robust bioinformatics approach has significantly improved the predictive values of our meta-analysis. Read More

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Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.

PLoS One 2021 13;16(5):e0251456. Epub 2021 May 13.

Department of Molecular Cell Biology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

Pbp1, the yeast ortholog of human Ataxin-2, was originally isolated as a poly(A) binding protein (Pab1)-binding protein. Pbp1 regulates the Pan2-Pan3 deadenylase complex, thereby modulating the mRNA stability and translation efficiency. However, the physiological significance of Pbp1 remains unclear since a yeast strain harboring PBP1 deletion grows similarly to wild-type strain on normal glucose-containing medium. Read More

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Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.

PLoS One 2021 13;16(5):e0251464. Epub 2021 May 13.

Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul, Republic of Korea.

Lumbar spinal stenosis (LSS), one of the most commonly reported spinal disorders, can cause loss of sensation and dyskinesia. In currently used animal models of LSS, the spinal cord is covered entirely with a silicone sheet, or block-shaped silicone is inserted directly into the spinal canal after laminectomy. However, the effects of differences between these implant materials have not been studied. Read More

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RACK1 modulates polyglutamine-induced neurodegeneration by promoting ERK degradation in Drosophila.

PLoS Genet 2021 May 13;17(5):e1009558. Epub 2021 May 13.

National Institute of Biological Sciences, Beijing, China.

Polyglutamine diseases are neurodegenerative diseases caused by the expansion of polyglutamine (polyQ) tracts within different proteins. Although multiple pathways have been found to modulate aggregation of the expanded polyQ proteins, the mechanisms by which polyQ tracts induced neuronal cell death remain unknown. We conducted a genome-wide genetic screen to identify genes that suppress polyQ-induced neurodegeneration when mutated. Read More

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Red blood cell phenotyping from 3D confocal images using artificial neural networks.

PLoS Comput Biol 2021 May 13;17(5):e1008934. Epub 2021 May 13.

Department of Experimental Physics, Saarland University, Campus E2.6, Saarbrücken, Germany.

The investigation of cell shapes mostly relies on the manual classification of 2D images, causing a subjective and time consuming evaluation based on a portion of the cell surface. We present a dual-stage neural network architecture for analyzing fine shape details from confocal microscopy recordings in 3D. The system, tested on red blood cells, uses training data from both healthy donors and patients with a congenital blood disease, namely hereditary spherocytosis. Read More

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Integrative methylome-transcriptome analysis unravels cancer cell vulnerabilities in infant MLL-rearranged B-cell acute lymphoblastic leukemia.

J Clin Invest 2021 May 13. Epub 2021 May 13.

Josep Carreras Leukemia Research Institute-Campus Clinic, University of Barcelona, Barcelona, Spain.

B-cell acute lymphoblastic leukemia (B-ALL) is the most common childhood cancer. As predicated by its prenatal origin, infant B-ALL (iB-ALL) shows an exceptionally silent DNA mutational landscape, suggesting that alternative epigenetic mechanisms may substantially contribute to its leukemogenesis. Here, we have integrated genome-wide DNA methylome and transcriptome data from 69 patients with de novo MLL-rearranged (MLLr) and non-MLLr iB-ALL leukemias uniformly treated according to Interfant-99/06 protocol. Read More

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ETV4/5 drive synovial sarcoma through control of the cell cycle and the DUX4 embryonic pathway.

J Clin Invest 2021 May 13. Epub 2021 May 13.

Department of Medicine, Division of Medical Oncology, University of Miami Miller School of Medicine, Miami, United States of America.

Synovial sarcoma is an aggressive malignancy with no effective treatments for patients with metastasis. The synovial sarcoma fusion, SS18-SSX, which recruits the SWI/SNF-BAF chromatin remodeling and polycomb repressive complexes, results in epigenetic activation of FGFR signaling. In genetic FGFR knockout models, culture, and xenograft synovial sarcoma models treated with the FGFR inhibitor BGJ398, we show that FGFR1, FGFR2, and FGFR3 were crucial for tumor growth. Read More

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stem extract confers robust antioxidant and antidiabetic effects against high fructose/streptozotocin induced type 2 diabetes in rats. Exploration of bioactive compounds using UHPLC-ESI-QTOF-MS.

Arch Physiol Biochem 2021 May 13:1-13. Epub 2021 May 13.

International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Thailand.

Diabetes mellitus is the most deadly and most prevalent metabolic disease of contemporary times. This study evaluated the antidiabetic, antioxidant, and pancreato-protective effects of extract (SIE) in high-fructose/streptozotocin-induced type 2 diabetes. SIE (50, 100, and 200 mg/kg) was administered to diabetic rats for 8 weeks, thereafter glycaemic parameters, pancreatic β cell function, lipid profile, hepatorenal function, and antioxidant parameters were evaluated in diabetic rats treated SIE. Read More

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Exogenous HS Protects Colon Cells in Ulcerative Colitis by Inhibiting and Activating Autophagy.

DNA Cell Biol 2021 May 13. Epub 2021 May 13.

Department of Gastroenterology, The Second Affiliated Hospital of Guilin Medical University, Guilin City, P.R. China.

Hydrogen sulfide (HS) has been reported to participate in intestinal mucosal defense and repair. However, the precise regulatory mechanisms of HS in ulcerative colitis (UC) remain unclear. We explored the effects of sodium hydrosulfide (NaHS), a donor of HS, in dextran sulfate sodium (DSS)-induced colitis in rats. Read More

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