268,904 results match your criteria cell markers


Neural stem cell delivery using brain-derived tissue-specific bioink for recovering from traumatic brain injury.

Biofabrication 2021 Sep 22. Epub 2021 Sep 22.

Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Kyungbuk 790-784, KOREA, Pohang, 37673, Korea (the Republic of).

Traumatic brain injury is one of the leading causes of accidental death and disability. The loss of parts in a severely injured brain induces edema, neuronal apoptosis, and neuroinflammation. Recently, stem cell transplantation demonstrated regenerative efficacy in an injured brain. Read More

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September 2021

Metformin enhances the osteogenesis and angiogenesis of human umbilical cord mesenchymal stem cells for tissue regeneration engineering.

Int J Biochem Cell Biol 2021 Sep 19:106086. Epub 2021 Sep 19.

Daxing Research Institute, University of Science and Technology Beijing. Beijing 100083, China; School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China. Electronic address:

Human umbilical cord mesenchymal stem cells (hUC-MSCs) are a potential clinical material in regenerative medicine applications. Metformin has shown safety and effectiveness as a clinical drug. However, the effect of metformin as a treatment on hUC-MSCs is unclear. Read More

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September 2021

Compromised nuclear envelope integrity drives TREX1-dependent DNA damage and tumor cell invasion.

Cell 2021 Sep 15. Epub 2021 Sep 15.

Institut Curie and Institut Pierre Gilles de Gennes, PSL Research University, CNRS, UMR 144, Paris, France. Electronic address:

Although mutations leading to a compromised nuclear envelope cause diseases such as muscular dystrophies or accelerated aging, the consequences of mechanically induced nuclear envelope ruptures are less known. Here, we show that nuclear envelope ruptures induce DNA damage that promotes senescence in non-transformed cells and induces an invasive phenotype in human breast cancer cells. We find that the endoplasmic reticulum (ER)-associated exonuclease TREX1 translocates into the nucleus after nuclear envelope rupture and is required to induce DNA damage. Read More

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September 2021

A Cre-driven allele-conditioning line to interrogate CD4 conventional T cells.

Immunity 2021 Sep 15. Epub 2021 Sep 15.

Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; Tumor Microenvironment Center, UPMC Hillman Cancer Center, Pittsburgh, PA 15232, USA; Cancer Immunology and Immunotherapy Program, UPMC Hillman Cancer Center, Pittsburgh, PA 15232, USA. Electronic address:

CD4 T cells share common developmental pathways with CD8 T cells, and upon maturation, CD4 T conventional T (Tconv) cells lack phenotypic markers that distinguish these cells from FoxP3 T regulatory cells. We developed a tamoxifen-inducible ThPOK line with Frt sites inserted on either side of the CreERT2-hCD2 cassette, and a Foxp3 strain, expressing Ametrine and FlpO in Foxp3 cells. Breeding these mice resulted in a CD4conv line that allows for the specific manipulation of a gene in CD4 Tconv cells. Read More

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September 2021

Brain endothelial cells-derived extracellular vesicles overexpressing ECRG4 inhibit glioma proliferation through suppressing inflammation and angiogenesis.

J Tissue Eng Regen Med 2021 Sep 22. Epub 2021 Sep 22.

Department of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.

Esophageal cancer related gene-4 (ECRG4) has been shown to be a candidate tumor suppressor in many tumors, but its role in glioma remains poorly understood. This study aimed to explore whether extracellular vesicles (EVs) derived from brain endothelial cells which overexpressed ECRG4 have anti-tumor effect on gliomas in vivo and in vitro, as well as the possible mechanism. A constructed lentivirus expressing the ECRG4 gene was transfected into the hCMEC/D3 cell line. Read More

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September 2021

Coordinated IgG2 and IgE responses as a marker of allergen immunotherapy efficacy.

Allergy 2021 Sep 22. Epub 2021 Sep 22.

Stallergenes Greer, Antony, France.

Background: IgG2 responses are associated with repeated antigen exposure and display highly mutated variable domains. A recent study highlighted a role of IgG2+ memory B cells and allergen-specific IgG2 levels after a 3 consecutive pre-seasonal sublingual allergen immunotherapy (AIT) with grass pollen tablet. Herein, we aim to explore changes in allergen-specific IgG2 in individuals undergoing house dust mite immunotherapy (HDM-AIT) and explore whether the interrelationship with other humoral responses (i. Read More

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September 2021

DNA damage-induced degradation of Sp1 promotes cellular senescence.

Geroscience 2021 Sep 22. Epub 2021 Sep 22.

Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N 15th Street, MS497, Philadelphia, PA, 19102, USA.

Persistent DNA damage (genotoxic stress) triggers signaling cascades that drive cells into apoptosis or senescence to avoid replicating a damaged genome. Sp1 has been found to play a role in double strand break (DSB) repair, and a link between Sp1 and aging has also been established, where Sp1 protein, but not RNA, levels decrease with age. Interestingly, inhibition ATM reverses the age-related degradation of Sp1, suggesting that DNA damage signaling is involved in senescence-related degradation of Sp1. Read More

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September 2021

Comparison of individual and combination treatments with naproxen, prednisolone and hydroxychloroquine to treat Complete Freund's Adjuvant induced arthritis.

Inflammopharmacology 2021 Sep 22. Epub 2021 Sep 22.

Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, P.O. Box 6231, Jeddah, 21442, Saudi Arabia.

Aim of this study was to evaluate and compare the efficacy of anti-arthritic drugs (naproxen, prednisolone, and hydroxychloroquine) alone and in combination. The in vitro anti-arthritic activity was evaluated by stabilization of human erythrocytes (HRBCs) membrane assays. In vivo activity was carried out using Complete Freund's Adjuvant (CFA) induced arthritic model in Wistar rat. Read More

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September 2021

Temporo-spatial distribution of stem cell markers CD146 and p75NTR during odontogenesis in mice.

J Appl Oral Sci 2021 20;29:e20210138. Epub 2021 Sep 20.

Universidade de São Paulo, Faculdade de Odontologia, Departamento de Estomatologia, Disciplina de Patologia Oral e Maxilofacial, Laboratório de Biologia de Células-Tronco em Odontologia LABITRON, São Paulo, SP, Brasil.

Mesenchymal and epithelial stem cells were identified in dental tissues; however, knowledge about the odontogenic stem cells is limited, and there are some questions regarding their temporo-spatial dynamics in tooth development.

Objective: Our study aimed to analyze the expression of the stem cell markers CD146 and p75NTR during the different stages of odontogenesis.

Methodology: The groups consisted of 13. Read More

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September 2021

The Protective Effects of Live and Pasteurized Akkermansia muciniphila and Its Extracellular Vesicles against HFD/CCl4-Induced Liver Injury.

Microbiol Spectr 2021 Sep 22:e0048421. Epub 2021 Sep 22.

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Akkermansia muciniphila, as a member of the gut microbiota, has been proposed as a next-generation probiotic. Liver fibrosis is the main determinant of liver dysfunction and mortality in patients with chronic liver disease. In this study, we aimed to determine the beneficial effects of live and pasteurized A. Read More

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September 2021

From Bench to Bedside: The Evolution of Genomics and Its Implications for the Current and Future Management of Multiple Myeloma.

Cancer J 2021 May-Jun 01;27(3):213-221

From the Perlmutter Cancer Center, NYU Langone Health, New York, NY.

Abstract: The summation of 20 years of biological studies and the comprehensive analysis of more than 1000 multiple myeloma genomes with data linked to clinical outcome has enabled an increased understanding of the pathogenesis of multiple myeloma in the context of normal plasma cell biology. This novel data have facilitated the identification of prognostic markers and targets suitable for therapeutic manipulation. The challenge moving forward is to translate this genetic and biological information into the clinic to improve patient care. Read More

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September 2021

PeacoQC: Peak-Based Selection of High Quality Cytometry Data.

Cytometry A 2021 Sep 22. Epub 2021 Sep 22.

Data Mining and Modeling for Biomedicine Group, VIB Center for Inflammation Research, Ghent, Belgium.

In cytometry analysis, a large number of markers is measured for thousands or millions of cells, resulting in high-dimensional datasets. During the measurement of these samples, erroneous events can occur such as clogs, speed changes, slow uptake of the sample etc. which can influence the downstream analysis and can even lead to false discoveries. Read More

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September 2021

[Therapeutic mechanism of the Mongolian medicine Powder against liver fibrosis based on UHPLC-TOF-MS combined with network pharmacological methods].

Authors:
C Zhang Y Yan X Gao Y Ma

Nan Fang Yi Ke Da Xue Xue Bao 2021 Aug;41(8):1131-1141

Department of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010059, China.

Objective: To elucidate the chemical composition of the Mongolian medicine Powder and explore its key targets, related pathways and its therapeutic mechanism for liver fibrosis.

Methods: UHPLC-TOF-MS was used to analyze the composition of Powder. The therapeutic targets of Powder were screened in Swiss Target Prediction database, and liver fibrosis-related targets were screened in TTD and GeneCards databases to identify the anti-fibrosis targets of Powder by intersection using Venny. Read More

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Short-term consumption of alcohol (vodka) mixed with energy drink (AMED) attenuated alcohol-induced cerebral capillary disturbances and neuroinflammation in adult wild-type mice.

Nutr Neurosci 2021 Sep 22:1-10. Epub 2021 Sep 22.

Curtin Health Innovation Research Institute, Faculty of Health Sciences, Curtin University, Perth, Australia.

The ingestion of combinatory Alcohol Mixed with Energy Drink (AMED) beverages continues to increase markedly, particularly among young adults. Some studies suggest detrimental health effects related to the combination of alcohol with energy drink formulations; however, the consumption of AMED has not been investigated in context of the cerebral microvasculature or neuroinflammation. We hypothesized that cerebral capillary integrity and glial cells are particularly vulnerable to the combination of AMED. Read More

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September 2021

Characterization of Urine Derived Stem Cells from Patients with End-Stage Liver Diseases and Application to Induced Acute and Chronic Liver Injury of Nude Mice Model.

Stem Cells Dev 2021 Sep 22. Epub 2021 Sep 22.

Chongqing Medical University Affiliated Children's Hospital, 159456, Chongqing, China;

Urine-derived stem cells (USCs) are adult stem cells isolated from urine with strong proliferative ability and differentiation potentials. Cell transplantation of USCs could partly repair liver injury. It has been reported that the proliferative ability of bone mesenchymal stem cells in patients with chronic liver failure is significantly lower than in patients without liver disease. Read More

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September 2021

Identification of predictive markers for the generation of well-differentiated human induced pluripotent stem cell-derived kidney organoids.

Stem Cells Dev 2021 Sep 22. Epub 2021 Sep 22.

Erasmus Medical Center, 6993, Erasmus MC Transplantation Institute, Rotterdam, Zuid-Holland, Netherlands;

Human-induced pluripotent stem cell (iPSC) derived kidney organoids have the potential to advance studies to kidney development and disease. However, reproducible generation of kidney organoids is a challenge. A large variability in the percentage of nephron structures and the expression of kidney specific genes was observed among organoids, showing no association with iPSC lines. Read More

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September 2021

Implication of mucus-secreting cells, acidophilic granulocytes and monocytes/macrophages in the resolution of skin inflammation caused by subcutaneous injection of λ/κ-carrageenin to gilthead seabream (Sparus aurata) specimens.

J Fish Dis 2021 Sep 22. Epub 2021 Sep 22.

Immunobiology for Aquaculture group, Department of Cell Biology and Histology. Faculty of Biology, Campus Regional de Excelencia Internacional 'Campus Mare Nostrum', University of Murcia, Murcia, Spain.

To date, the mechanisms of inflammation have been poorly studied in fish of commercial interest, due to the lack of development of appropriate experimental models. The current study evaluated a local inflammation triggered by a polymeric carrageenin mixture (a mucopolysaccharide derived from the red seaweed Chondrus crispus) in the skin of gilthead seabream (Sparus aurata). Fish were injected subcutaneously with phosphate-buffered saline (as control) or λ/κ-carrageenin (1%), and skin samples from the injection sites were collected 1. Read More

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September 2021

Articular cartilage degeneration and bone adaptation due to lack of dystrophin in mice.

J Bone Miner Metab 2021 Sep 22. Epub 2021 Sep 22.

Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Rua Silva Jardim, 136 Lab 328, CEP: 11015-020, Santos, SP, 11060-001, Brazil.

Introduction: Duchenne muscular dystrophy is caused by the absence of dystrophin. This study aimed to investigate femoral morphological characteristics of lack of dystrophin in MDX mice, considering that this model, different from DMD patient, is not influenced by corticosteroids administration and limited ambulation.

Materials And Methods: Proximal femur of male 16-week-old Control and MDX mice were submitted to histological, morphometric (volume density of articular cartilage, compact bone, trabecular bone and bone marrow; articular cartilage layers area; articular cartilage cell area), and immunohistochemistry analysis for RUNX-2, RANK-L, MMP-2, MMP-9, Caspase-3 and KI-67. Read More

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September 2021

Impact of rituximab on COVID-19 outcomes.

Ann Hematol 2021 Sep 22. Epub 2021 Sep 22.

Tisch Cancer Institute, New York, NY, USA.

Rituximab is associated with prolonged B-cell depletion and secondary hypogammaglobulinemia and is associated with a dampened humoral response and increased infectious complications. To describe the potential impact of prior rituximab therapy on clinical outcomes from SARS-CoV-2 infection and development of COVID-19 antibodies, we conducted a retrospective study of adults across the Mount Sinai Health System diagnosed with COVID-19 who received rituximab for any indication from February 2019 to October 2020. Patients' baseline characteristics, markers of disease severity, clinical outcomes, and antibody development were examined. Read More

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September 2021

Differentiation of human induced pluripotent stem cells into testosterone-producing Leydig-like cells.

Endocrinology 2021 Sep 21. Epub 2021 Sep 21.

Division of Advanced Medical Science, Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.

Late-onset hypogonadism (LOH) syndrome due to a partial lack of testosterone, which is mainly secreted by Leydig cells in the testes, decreases the quality of life of older men. Leydig cell transplantation is expected to be a promising alternative to conventional testosterone replacement therapy (TRT) for LOH syndrome. We herein report a simple and robust protocol for directed differentiation of human induced pluripotent stem cells (hiPSCs) into Leydig-like cells by doxycycline-inducible overexpression of NR5A1 and treatment with a combination of 8-bromoadenosine-3',5'-cyclic monophosphate (8-Br-cAMP) and forskolin. Read More

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September 2021

Transgenic reporter analysis of ChIP-Seq-defined enhancers identifies novel target genes for the terminal selector UNC-3/Collier/Ebf.

MicroPubl Biol 2021 14;2021. Epub 2021 Sep 14.

Department of Neurobiology, University of Chicago, Chicago, IL, USA.

Terminal selector-type transcription factors are key regulators of neuronal identity and function (Hobert and Kratsios, 2019; Kratsios and Hobert, 2018). Mechanistically, terminal selectors are thought to act directly through binding at the -regulatory region of genes (termed "terminal identity genes") that encode, among others, neurotransmitter [NT] synthesis proteins, ion channels, neuropeptides, and cell adhesion molecules (Hobert and Kratsios, 2019; Kratsios and Hobert, 2018). Although dozens of terminal selectors have been described thus far for individual neuron types of the nematode (Hobert, 2016), the identification of their target genes has primarily relied on candidate approaches and availability of markers for neuronal terminal identity. Read More

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September 2021

Profiling of Androgen-Dependent Enhancer RNAs Expression in Human Prostate Tumors: Search for Malignancy Transition Markers.

Res Rep Urol 2021 14;13:705-713. Epub 2021 Sep 14.

Research Institute of Innovative Medicine, Tokiwa Foundation, Iwaki, Japan.

Introduction: Although the ability of androgens to promote prostate cancer development has been known for decades, the molecular mechanisms of androgen receptor (AR) signaling in the tumorigenesis remain unclear. Enhancer RNAs (eRNAs) transcribed from strong enhancers, or super-enhancers (SEs), have recently emerged as a novel class of regulatory non-coding RNAs (ncRNAs) that facilitate transcription, including that of androgen target genes, through chromatin looping to position enhancers proximate to the promoters. The aim of this study was to assess androgen-dependent transcription in prostate tumors of eRNAs (designated as KLK3eRNAs) from the SE of the gene encoding the prostate-specific antigen (PSA) protein, a clinical marker of prostate carcinogenesis. Read More

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September 2021

Chronic exposure to tramadol induces cardiac inflammation and endothelial dysfunction in mice.

Sci Rep 2021 Sep 21;11(1):18772. Epub 2021 Sep 21.

Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Assiut, Egypt.

Tramadol is an opioid extensively used to treat moderate to severe pain; however, prolonged therapy is associated with several tissues damage. Chronic use of tramadol was linked to increased hospitalizations due to cardiovascular complications. Limited literature has described the effects of tramadol on the cardiovascular system, so we sought to investigate these actions and elucidate the underlying mechanisms. Read More

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September 2021

Reconstructing aspects of human embryogenesis with pluripotent stem cells.

Nat Commun 2021 Sep 21;12(1):5550. Epub 2021 Sep 21.

Plasticity and Self-Organization Group, Division of Biology and Biological Engineering, Caltech, Pasadena, CA, 91125, USA.

Understanding human development is of fundamental biological and clinical importance. Despite its significance, mechanisms behind human embryogenesis remain largely unknown. Here, we attempt to model human early embryo development with expanded pluripotent stem cells (EPSCs) in 3-dimensions. Read More

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September 2021

DNA methylation-based signature of CD8+ tumor-infiltrating lymphocytes enables evaluation of immune response and prognosis in colorectal cancer.

J Immunother Cancer 2021 Sep;9(9)

Guangdong Institute of Gastroenterology, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

Background: Tumor-infiltrating lymphocytes (TILs), especially CD8+ TILs, can be used for predicting immunotherapy responsiveness and survival outcome. However, the evaluation of CD8+ TILs currently relies on histopathological methodology with high variability. We therefore aimed to develop a DNA methylation signature for CD8+ TILs (CD8+ MeTIL) that could evaluate immune response and prognosis in colorectal cancer (CRC). Read More

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September 2021

Expression and subcellular localization of Discoidin Domain Receptor 1 (DDR1) define prostate cancer aggressiveness.

Cancer Cell Int 2021 Sep 21;21(1):507. Epub 2021 Sep 21.

Department of Pathology, Wayne State University School of Medicine and Karmanos Cancer Institute, Scott Hall #8200, 540 E. Canfield St, Detroit, MI, 48201, USA.

Background: The Discoidin Domain Receptor 1 (DDR1) is one of the two members of a unique family of receptor tyrosine kinase receptors that signal in response to collagen, which has been implicated in cancer progression. Here, we examined the expression of DDR1 in prostate cancer (PCa), and assessed its potential value as a prognostic marker, as a function of grade, stage and other clinicopathologic parameters.

Methods: We investigated the association between the expression level and subcellular localization of DDR1 protein and PCa aggressiveness by immunohistochemistry, using tissue microarrays (TMAs) encompassing 200 cases of PCa with various Gleason scores (GS) and pathologic stages with matched normal tissue, and a highly specific monoclonal antibody. Read More

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September 2021

Preoperative C-reactive protein to albumin ratio predicts anastomotic leakage after esophagectomy for thoracic esophageal cancer: a single-center retrospective cohort study.

BMC Surg 2021 Sep 21;21(1):348. Epub 2021 Sep 21.

Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan.

Background: Postoperative anastomotic leakage (AL) is associated with not only prolonged hospital stay and increased medical costs, but also poor prognosis in esophageal cancer. Several studies have addressed the utility of various inflammation-based and/or nutritional markers as predictors for postoperative complications. However, none have been documented as specific predictors for AL in esophageal cancer. Read More

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September 2021

Non-integrating episomal vectors induced pluripotent stem cell line (SDPHi001-A) from a healthy female individual.

Stem Cell Res 2021 Sep 16;56:102540. Epub 2021 Sep 16.

Department of General Surgery, The 960th Hospital of the PLA Joint Logistics Support Force, Jinan, Shandong 250031, China. Electronic address:

Isolated peripheral blood mononuclear cells (PBMCs) from a healthy female were successfully transformed into induced pluripotent stem cells (iPSCs) by non-integrating episomal vectors containing OCT4, SOX2, c-MYC, KLF4, and BCL-XL. The iPSCs displayed a normal karyotype, expressed pluripotency markers and could differentiate into three germ layers in vitro. This iPSC line may be subsequently used for further researches. Read More

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September 2021

Current understanding of hexavalent chromium [Cr(VI)] neurotoxicity and new perspectives.

Environ Int 2021 Sep 18;158:106877. Epub 2021 Sep 18.

Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40292, USA; Pediatric Research Institute, The Department of Pediatrics, University of Louisville School of Medicine, Louisville, KY 40292, USA.

Hexavalent chromium [Cr(VI)] is a global environmental pollutant that increases risk for several types of cancers and is increasingly being recognized as a neurotoxicant. Traditionally, the brain has been viewed as a largely post-mitotic organ due to its specialized composition of neurons, and consequently, clastogenic effects were not considered in neurotoxicology. Today, we understand the brain is composed of at least eight distinct cell types - most of which continue mitotic activity throughout lifespan. Read More

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September 2021

ATF4-mediated transcriptional regulation protects against β-cell loss during endoplasmic reticulum stress in a mouse model.

Mol Metab 2021 Sep 18:101338. Epub 2021 Sep 18.

Division of Molecular Biology, Institute for Genome Research, Institute of Advanced Medical Sciences, Tokushima University, Tokushima, 770-8503, Japan; Fujii Memorial Institute of Medical Sciences, Institute of Advanced Medical Sciences, Tokushima University, Tokushima, 770-8503, Japan; Department of Molecular Research, Diabetes Therapeutics and Research Center, Institute of Advanced Medical Sciences, Tokushima University, Tokushima, 770-8503, Japan. Electronic address:

Objective: Activating transcription factor 4 (ATF4) is a transcriptional regulator of the unfolded protein response and integrated stress response (ISR) that promote the restoration of normal endoplasmic reticulum (ER) function. Previous reports demonstrated that dysregulation of the ISR showed development of severe diabetes. However, the contribution of ATF4 to pancreatic beta cells remains poorly understood. Read More

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September 2021