848 results match your criteria cell landing

Carbon nanohorns as nanocontainers for cisplatin: insight into their interaction with the plasma membranes of normal and breast cancer cells.

Phys Chem Chem Phys 2021 Jul 27. Epub 2021 Jul 27.

Programa de Pós-graduação em Modelagem Computacional (PGMC), Departamento de Ciência da Computação, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora (UFJF), Campus Universitário, Martelos, Juiz de Fora, MG 36036-330, Brazil.

Cisplatin (cddp)-based chemotherapy is one of the most effective therapeutic alternatives for breast cancer treatment, the most common form of cancer, despite the severe side effects related to the high toxicity and low selectivity of cddp. To circumvent these drawbacks, the encapsulation of cddp into oxidized carbon nanohorns (CNHoxs) has been shown as a promising formulation with biocompatibility and low toxicity. However, there is still a lack of studies regarding the behavior of this [email protected] nanovector on the cell membranes. Read More

View Article and Full-Text PDF

Hijacking of the aircraft OK ADN L410 TURBOLET on June 8, 1972 - Forensic Medical Notes.

Soud Lek 2021 ;66(1):11-16

On 8 June 1972, the Czechoslovak OK DNN aircraft carrier L410 Turbolet was delivered to the regular line Marianske Lazne - Prague to the Federal Republic of Germany. About 8 minutes after the launch from Marianske Lazne Airport, the likely head of the group of kidnappers threw the captain of the plane. During the fight with one of the passenger, the head of the group of kidnappers killed the captain of the aircraft by a shot from 7. Read More

View Article and Full-Text PDF

A heuristic model-based approach for compensating wind effects in ski jumping.

J Biomech 2021 Jun 24;125:110585. Epub 2021 Jun 24.

University of Jyväskylä, Faculty of Sport and Health Sciences, Biology of Physical Activity, PO Box 35, 40014 Jyväskylä, Finland.

Wind influences the jump length in ski jumping, which raises questions about the fairness. To counteract the wind problem, the International Ski Federation has introduced a wind compensation system in 2009: time-averaged wind velocity components tangential to the landing slope are obtained from several sites along the landing slope, and these data are used in a linear statistical model for estimating the jump length effect of wind. This is considered in the total score of the ski jump. Read More

View Article and Full-Text PDF

Crash landing of by MSHA pili-assisted braking and anchoring in a viscoelastic environment.

Elife 2021 Jul 2;10. Epub 2021 Jul 2.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.

Mannose-sensitive hemagglutinin (MSHA) pili and flagellum are critical for the surface attachment of , the first step of colonization on host surfaces. However, the cell landing mechanism remains largely unknown, particularly in viscoelastic environments such as the mucus layers of intestines. Here, combining the cysteine-substitution-based labeling method with single-cell tracking techniques, we quantitatively characterized the landing of by directly observing both pili and flagellum of cells in a viscoelastic non-Newtonian solution consisting of 2% Luria-Bertani and 1% methylcellulose (LB+MC). Read More

View Article and Full-Text PDF

Hidrox and Chronic Cystitis: Biochemical Evaluation of Inflammation, Oxidative Stress, and Pain.

Antioxidants (Basel) 2021 Jun 29;10(7). Epub 2021 Jun 29.

Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy.

Interstitial cystitis/painful bladder syndrome (IC/PBS) is a chronic bladder condition characterized by frequent urination, inflammation, oxidative stress, and pain. The aim of the study was to evaluate the anti-inflammatory and antioxidant effects of an oral administration of Hidrox (10 mg/kg) in the bladder and spinal cord in a rodent model of IC/BPS. The chronic animal model of cystitis was induced by repeated intraperitoneal injections of cyclophosphamide (CYP) for five consecutive days. Read More

View Article and Full-Text PDF

Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay.

Int J Environ Res Public Health 2021 06 9;18(12). Epub 2021 Jun 9.

Department of Natural Sciences, University of Maryland Eastern Shore, 1 Backbone Road, Princess Anne, MD 21853, USA.

Contaminants of Emerging Concern (CECs) with estrogenic or estrogenic-like activity have been increasingly detected in aquatic environments and have been an issue of global concern due to their potential negative effects on wildlife and human health. This study used the MCF-7 cell proliferation assay (E-Screen) to assess the estrogenic activity profiles in Maryland Coastal Bays (MCBs), a eutrophic system of estuaries impacted by human activities. Estrogenic activity was observed in all study sites tested. Read More

View Article and Full-Text PDF

Nrf2 Activation Attenuates Acrylamide-Induced Neuropathy in Mice.

Int J Mol Sci 2021 Jun 1;22(11). Epub 2021 Jun 1.

Department of Occupational and Environmental Health, Tokyo University of Science, Noda 278-8510, Japan.

Acrylamide is a well characterized neurotoxicant known to cause neuropathy and encephalopathy in humans and experimental animals. To investigate the role of nuclear factor erythroid 2-related factor 2 (Nrf2) in acrylamide-induced neuropathy, male C57Bl/6JJcl adult mice were exposed to acrylamide at 0, 200 or 300 ppm in drinking water and co-administered with subcutaneous injections of sulforaphane, a known activator of the Nrf2 signaling pathway at 0 or 25 mg/kg body weight daily for 4 weeks. Assessments for neurotoxicity, hepatotoxicity, oxidative stress as well as messenger RNA-expression analysis for Nrf2-antioxidant and pro-inflammatory cytokine genes were conducted. Read More

View Article and Full-Text PDF

Streptococcus dysgalactiae: A pathogen of feral populations of silver carp Hypophthalmichthys molitrix from a fish kill event.

J Aquat Anim Health 2021 Jun 29. Epub 2021 Jun 29.

Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Stoneville, MS, 39762, USA.

In August of 2018, a series of large fish kills occurred on the Mississippi River in North Louisiana involving only silver carp Hypophthalmichthys molitrix. Clinical signs observed in moribund animals included: erratic swimming behavior, such as spiraling and spinning at the surface. A moribund specimen was captured by dip net near the surface at Lake Providence Landing in East Carroll Parish in North Louisiana and submitted for analysis. Read More

View Article and Full-Text PDF

Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins.

ACS Synth Biol 2021 Jul 16;10(7):1715-1727. Epub 2021 Jun 16.

Department of Biological Sciences, KAIST, Daejeon 34141, Republic of Korea.

A platform, based on targeted integration of transgenes using recombinase-mediated cassette exchange (RMCE) coupled with CRISPR/Cas9, is increasingly being used for the development of mammalian cell lines that produce therapeutic proteins, because of reduced clonal variation and predictable transgene expression. However, low efficiency of the RMCE process has hampered its application in multicopy or multisite integration of transgenes. To improve RMCE efficiency, nuclear transport of RMCE components such as site-specific recombinase and donor plasmid was accelerated by incorporation of nuclear localization signal and DNA nuclear-targeting sequence, respectively. Read More

View Article and Full-Text PDF

The red eye.

Stefano Bonini

Eur J Ophthalmol 2021 Jun 12:11206721211024827. Epub 2021 Jun 12.

Department of Ophthalmology, University of Rome Campus BioMedico, Rome, Italy.

The red eye is one of the most common cause encountered in ophthalmic practice but a red eye is not always related to eye diseases, instead, it may be a clinical sign of several systemic diseases which may the alarmin signal of sight-threatening or life-threatening condition. Frequently, GPs, pediatricians, immunologists, and rheumatologists are the first landing of patients with a "red eye." This paper is addressed to non-ophthalmic specialists who may be faced with patients having a red eye. Read More

View Article and Full-Text PDF

Hidrox and Endometriosis: Biochemical Evaluation of Oxidative Stress and Pain.

Antioxidants (Basel) 2021 May 4;10(5). Epub 2021 May 4.

Department of Biomedical, Dental and Morphological and Functional Imaging University of Messina, Via Consolare Valeria, 98125 Messina, Italy.

Endometriosis is a gynecological and painful condition affecting women of reproductive age. It is characterized by dysfunctional endometrium-like implants outside of the uterine cavity. The purpose of this study was to evaluate the effects of Hidrox, an aqueous extract of olive pulp containing hydroxytyrosol, on endometriotic lesions associated with pro-oxidative alterations and pain-like behaviors. Read More

View Article and Full-Text PDF

Two swimming modes in Trachymedusae; bell kinematics and the role of giant axons.

J Exp Biol 2021 05 25;224(10). Epub 2021 May 25.

School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol BS8 1TD, UK.

Although members of the Rhopalonematidae family (Cnidaria, Hydrozoa, Trachymedusae) are known to exhibit unusually powerful jet swimming in addition to their more normal slow swimming behaviour, for the most part, reports are rare and anecdotal. Many species are found globally at depths of 600-2000 m, and so observation and collection depend on using remotely operated submersible vehicles. With a combination of in situ video footage and laboratory measurements, we have quantified kinematic aspects of this dual swimming motion and its electrophysiology. Read More

View Article and Full-Text PDF

Oviposition Response of Monarch Butterfly (Lepidoptera: Nymphalidae) to Imidacloprid-Treated Milkweed.

Environ Entomol 2021 06;50(3):541-549

Department of Natural Resource Ecology and Management, Iowa State University, 339 Science Hall II, 2310 Pammel Drive, Ames, IA 50011, USA.

Monarch butterfly (Danaus plexippus) populations have declined over the last two decades, attributable in part to declines in its larval host plant, milkweed (Asclepias spp.), across its breeding range. Conservation efforts in the United States call for restoration of 1. Read More

View Article and Full-Text PDF

Estimating Contact Rates Between -Exposed Males With Female .

Front Cell Infect Microbiol 2021 29;11:616679. Epub 2021 Apr 29.

Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Laboratorio de Biomedicina Molecular, Ciudad Reynosa, Mexico.

Introduction: Effective control of will reduce the frequency and severity of outbreaks of dengue, chikungunya, and Zika; however, control programs are increasingly threatened by the rapid development of insecticide resistance. Thus, there is an urgent need for novel vector control tools, such as auto-dissemination of the entomopathogenic fungi and The aim of this study was to estimate contact rates of -exposed males with wild female . As a control the contact rates of untreated males with wild females was contrasted. Read More

View Article and Full-Text PDF

Species and population specific gene expression in blood transcriptomes of marine turtles.

BMC Genomics 2021 May 13;22(1):346. Epub 2021 May 13.

Department of Environmental Conservation, University of Massachusetts, Amherst, MA, USA.

Background: Transcriptomic data has demonstrated utility to advance the study of physiological diversity and organisms' responses to environmental stressors. However, a lack of genomic resources and challenges associated with collecting high-quality RNA can limit its application for many wild populations. Minimally invasive blood sampling combined with de novo transcriptomic approaches has great potential to alleviate these barriers. Read More

View Article and Full-Text PDF

AFM force-clamp spectroscopy captures the nanomechanics of the Tad pilus retraction.

Nanoscale Horiz 2021 06;6(6):489-496

Louvain Institute of Biomolecular Science and Technology, UCLouvain, Croix du Sud, 4-5, bte, L7.07.07, Louvain-la-Neuve B-1348, Belgium.

Motorization of bacterial pili is key to generate traction forces to achieve cellular function. The Tad (or Type IVc) pilus from Caulobacter crescentus is a widespread motorized nanomachine crucial for bacterial survival, evolution and virulence. An unusual bifunctional ATPase motor drives Tad pilus retraction, which helps the bacteria to land on target surfaces. Read More

View Article and Full-Text PDF

Hijacking of the aircraft OK ADN L410 TURBOLET on June 8, 1972 - Forensic Medical Notes.

Cesk Patol 2021 ;57(1):11-16

On 8 June 1972, the Czechoslovak OK DNN aircraft carrier L410 Turbolet was delivered to the regular line Marianske Lazne - Prague to the Federal Republic of Germany. About 8 minutes after the launch from Marianske Lazne Airport, the likely head of the group of kidnappers threw the captain of the plane. During the fight with one of the passenger, the head of the group of kidnappers killed the captain of the aircraft by a shot from 7. Read More

View Article and Full-Text PDF

Reproduction Multitasking: The Male Gametophyte.

Annu Rev Plant Biol 2021 06 26;72:581-614. Epub 2021 Apr 26.

Laboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences, 165 02 Prague 6, Czech Republic; email:

The gametophyte represents the sexual phase in the alternation of generations in plants; the other, nonsexual phase is the sporophyte. Here, we review the evolutionary origins of the male gametophyte among land plants and, in particular, its ontogenesis in flowering plants. The highly reduced male gametophyte of angiosperm plants is a two- or three-celled pollen grain. Read More

View Article and Full-Text PDF

Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule.

Biotechnol Prog 2021 Apr 23:e3158. Epub 2021 Apr 23.

Cell Line Development, Biotherapeutics Pharmaceutical Sciences, Pfizer Inc., Andover, Massachusetts, USA.

Site specific integration (SSI) expression systems offer robust means of generating highly productive and stable cell lines for traditional monoclonal antibodies. As complex modalities such as antibody-like molecules comprised of greater than two peptides become more prevalent, greater emphasis needs to be placed on the ability to produce appreciable quantities of the correct product of interest (POI). The ability to screen several transcript stoichiometries could play a large role in ensuring high amounts of the correct POI. Read More

View Article and Full-Text PDF

Genetic ablation of Nrf2 exacerbates neurotoxic effects of acrylamide in mice.

Toxicology 2021 05 16;456:152785. Epub 2021 Apr 16.

Department of Occupational and Environmental Health, Tokyo University of Science, Noda, Japan. Electronic address:

Acrylamide (ACR), a recognized neurotoxicant in humans and experimental animals, is widely used in industry and in food generated through Maillard reaction. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a master regulator of the cellular defense system and activates antioxidants and cytoprotective genes. The exact roles of Nrf2 in environmental electrophile-induced neurotoxicity is poorly understood. Read More

View Article and Full-Text PDF

Strange eyes, stranger brains: exceptional diversity of optic lobe organization in midwater crustaceans.

Proc Biol Sci 2021 04 7;288(1948):20210216. Epub 2021 Apr 7.

Department of Invertebrate Zoology, Smithsonian National Museum of Natural History, Washington, DC 20013, USA.

Nervous systems across Animalia not only share a common blueprint at the biophysical and molecular level, but even between diverse groups of animals the structure and neuronal organization of several brain regions are strikingly conserved. Despite variation in the morphology and complexity of eyes across malacostracan crustaceans, many studies have shown that the organization of malacostracan optic lobes is highly conserved. Here, we report results of divergent evolution to this 'neural ground pattern' discovered in hyperiid amphipods, a relatively small group of holopelagic malacostracan crustaceans that possess an unusually wide diversity of compound eyes. Read More

View Article and Full-Text PDF

Cryo-EM structure of the yeast TREX complex and coordination with the SR-like protein Gbp2.

Elife 2021 03 31;10. Epub 2021 Mar 31.

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, United States.

The evolutionarily conserved TRanscript-EXport (TREX) complex plays central roles during mRNP (messenger ribonucleoprotein) maturation and export from the nucleus to the cytoplasm. In yeast, TREX is composed of the THO sub-complex (Tho2, Hpr1, Tex1, Mft1, and Thp2), the DEAD box ATPase Sub2, and Yra1. Here we present a 3. Read More

View Article and Full-Text PDF

Ancestral developmental potentials in early bony fish contributed to vertebrate water-to-land transition.

Zool Res 2021 03;42(2):135-137

BGI-Shenzhen, Shenzhen, Guangdong 518083, China.

The water-to-land transition was a major step in vertebrate evolution and eventually gave rise to the tetrapods, including amphibians, reptiles, birds, and mammals. The first land invasion of our fish ancestors is considered to have occurred during the late Devonian period ~370 million years ago (Daeschler et al., 2006). Read More

View Article and Full-Text PDF

Selective Uptake of Pelagic Microbial Community Members by Caribbean Reef Corals.

Appl Environ Microbiol 2021 04 13;87(9). Epub 2021 Apr 13.

Monterey Bay Aquarium Research Institute, Moss Landing, California, USA

Coral reefs are possible sinks for microbes; however, the removal mechanisms at play are not well understood. Here, we characterize pelagic microbial groups at the CARMABI reef (Curaçao) and examine microbial consumption by three coral species: , , and Flow cytometry analyses of water samples collected from a depth of 10 m identified 6 microbial groups: , three groups of , photosynthetic eukaryotes, and heterotrophic bacteria. Minimum growth rates (μ) for , all groups, and photosynthetic eukaryotes were 0. Read More

View Article and Full-Text PDF

Will Fly Repellency Using Deltamethrin Reduce Intramammary Infections, Stress and Fatigue Indicators of Dairy Ewes under Intensive Management?

Pathogens 2021 Feb 19;10(2). Epub 2021 Feb 19.

Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

Intramammary infections (IMIs) caused by various pathogens may lead to clinical or subclinical mastitis, challenging the health and welfare status of infected animals and decreasing the quantity and quality of the produced milk. Additionally, the zoonotic potential of some of the pathogens isolated from IMI cases, the emergence of antibiotic resistance due to the extensive antibiotic use for IMI treatment, and the accumulation of antibiotic residues in milk and meat represent significant concerns for public health. Therefore, the investigation of IMI risk factors and the proposal of efficient measures to mitigate their effects on animal health and welfare is crucial. Read More

View Article and Full-Text PDF
February 2021

Development of a targeted integration Chinese hamster ovary host directly targeting either one or two vectors simultaneously to a single locus using the Cre/Lox recombinase-mediated cassette exchange system.

Biotechnol Prog 2021 Mar 5:e3140. Epub 2021 Mar 5.

Department of Cell Culture and Bioprocess Operations, Genentech, Inc., San Francisco, California, USA.

Cell line development (CLD) by random integration (RI) can be labor intensive, inconsistent, and unpredictable due to uncontrolled gene integration after transfection. Unlike RI, targeted integration (TI) based CLD introduces the antibody-expressing cassette to a predetermined site by recombinase-mediated cassette exchange (RMCE). The key to success for the development of a TI host for therapeutic antibody production is to identify a transcriptionally active hotspot that enables highly efficient RMCE and antibody expression with good stability. Read More

View Article and Full-Text PDF

: Application and Limitations of Deep Mutational Scanning for Genomic Missense Variant Function.

Drug Metab Dispos 2021 May 3;49(5):395-404. Epub 2021 Mar 3.

Division of Clinical Pharmacology, Department of Molecular Pharmacology and Experimental Therapeutics (L.Z., M.-F.H., I.M., L.W., R.M.W.), Division of Biomedical Statistics and Informatics, Department of Health Sciences Research (V.S., K.R.K.), and Mayo Clinic Center for Individualized Medicine (L.W., R.M.W.), Mayo Clinic, Rochester, Minnesota

() is an important transmembrane hepatic uptake transporter. Genetic variants in the gene have been associated with altered protein folding, resulting in protein degradation and decreased transporter activity. Next-generation sequencing (NGS) of pharmacogenes is being applied increasingly to associate variation in drug response with genetic sequence variants. Read More

View Article and Full-Text PDF

Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy.

Sci Rep 2021 Mar 2;11(1):4984. Epub 2021 Mar 2.

Department of Biomedical Engineering, Texas A&M University, College Station, USA.

We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma. FLIM can be used to monitor or detect changes in the fluorescence lifetime of metabolic cofactors (e.g. Read More

View Article and Full-Text PDF

A versatile platform for locus-scale genome rewriting and verification.

Proc Natl Acad Sci U S A 2021 Mar;118(10)

Institute for Systems Genetics, NYU Langone Health, New York, NY 10016.

Routine rewriting of loci associated with human traits and diseases would facilitate their functional analysis. However, existing DNA integration approaches are limited in terms of scalability and portability across genomic loci and cellular contexts. We describe Big-IN, a versatile platform for targeted integration of large DNAs into mammalian cells. Read More

View Article and Full-Text PDF

CHOK1SV GS-KO SSI expression system: A combination of the Fer1L4 locus and glutamine synthetase selection.

Biotechnol Prog 2021 Feb 20:e3137. Epub 2021 Feb 20.

R&D Cell Engineering, Lonza Biologics, Little Chesterford, UK.

There are an ever-increasing number of biopharmaceutical candidates in clinical trials fueling an urgent need to streamline the cell line development process. A critical part of the process is the methodology used to generate and screen candidate cell lines compatible with GMP manufacturing processes. The relatively large amount of clone phenotypic variation observed from conventional "random integration" (RI)-based cell line construction is thought to be the result of a combination of the position variegation effect, genome plasticity and clonal variation. Read More

View Article and Full-Text PDF
February 2021