1,368,885 results match your criteria cell growth

Hydrogel from acellular porcine adipose tissue promotes survival of adipose tissue transplantation.

Biomed Mater 2021 Apr 19. Epub 2021 Apr 19.

Department of Breast Surgery, Clinical Research Center for Breast, Sichuan University West China Hospital, No.37 Guoxuexiang, Chengdu, Sichuan, China, Chengdu, Sichuan, 610041, CHINA.

Lipofilling is a popular technique for soft tissue augmentation, limited by unpredictable graft survival. This study aimed at exploring the effect of hydrogel from acellular porcine adipose tissue (HAPA) on angiogenesis and survival of adipose tissue used for lipofilling. The effect of HAPA on adipose-derived stem cells (ADSCs) proliferation, adipogenic differentiation, and vascular endothelial growth factor (VEGF) secretion were evaluated in hypoxia and normoxia in vitro. Read More

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The intervertebral disc from embryonic development to disc degeneration: insights into spatial cellular organization.

Spine J 2021 Apr 16. Epub 2021 Apr 16.

Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, D-72072 Tübingen, Germany; Department of Orthopaedic Surgery, University Hospital of Tübingen, D-72076 Tübingen, Germany. Electronic address:

Background Context: Low back pain is commonly attributed to intervertebral disc (IVD) degeneration. IVD resembles articular cartilage in its biochemical and cellular composition in many ways. For articular cartilage, degeneration stage-specific characteristic spatial chondrocyte patterns have recently been described. Read More

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Short- and Long-term Probiotic Effects of Enterococcus hirae Isolated from Fermented Vegetable Wastes on the Growth, Immune Responses, and Disease Resistance of Hybrid Catfish (Clarias gariepinus × Clarias macrocephalus).

Fish Shellfish Immunol 2021 Apr 16. Epub 2021 Apr 16.

Laboratory of Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok, Thailand; Center of Excellence in Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok, Thailand. Electronic address:

This study evaluated the short- and long-term effects of dietary supplementation with Enterococcus hirae strain UPM02 on the growth performance, immunity, and disease resistance of hybrid catfish (Clarias gariepinus × Clarias macrocephalus) against Aeromonas hydrophila infection. In the long-term trial, fingerling fish were fed diets containing 0 (control), 2×10, or 2×10 CFU/g E. hirae UPM02 for 120 days. Read More

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Neurotoxicity of zearalenone's metabolites and beauvericin mycotoxins via apoptosis and cell cycle disruption.

Toxicology 2021 Apr 16:152784. Epub 2021 Apr 16.

Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, València, Spain.

Cell cycle progression and programmed cell death are imposed by pathological stimuli of extrinsic or intrinsic including the exposure to neurotoxins, oxidative stress and DNA damage. All can cause abrupt or delayed cell death, inactivate normal cell survival or cell death networks. Nevertheless, the mechanisms of the neuronal cell death are unresolved. Read More

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Biocompatible Ionic Liquids Assisted Transdermal Co-Delivery of Antigenic Protein and Adjuvant for Cancer Immunotherapy.

Int J Pharm 2021 Apr 16:120582. Epub 2021 Apr 16.

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Advanced Transdermal Drug Delivery System Center, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Division of Biotechnology, Center for Future Chemistry, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address:

Human skin contains numerous antigen-presenting cells that are a potential target for several immune-based therapies, including vaccination and cancer immunotherapy. However, the outermost layer of the skin-the stratum corneum-acts as a major physical barrier against the permeation of antigens that have a molecular weight > 500 Da. In this study, an ionic liquid-assisted delivery system (ILDS) was developed, which enabled the successful transdermal delivery of an antigenic protein, ovalbumin (OVA), with a toll-like receptor agonist, imiquimod, as an adjuvant, to stimulate a specific immune response. Read More

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Genome wide CRISPR screening reveals a role for sialylation in the tumorigenesis and chemoresistance of acute myeloid leukemia cells.

Cancer Lett 2021 Apr 16. Epub 2021 Apr 16.

Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address:

Aberrant activation of cytokine and growth factor signal transduction pathways confers enhanced survival and proliferation properties to acute myeloid leukemia (AML) cells. However, the mechanisms underlying the deregulation of signaling pathways in leukemia cells are unclear. To identify genes capable of independently supporting cytokine-independent growth, we employed a genome-wide CRISPR/Cas9-mediated loss-of-function screen in GM-CSF-dependent human AML TF-1 cells. Read More

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SIRT3 inhibits gallbladder cancer by induction of AKT-dependent ferroptosis and blockade of epithelial-mesenchymal transition.

Cancer Lett 2021 Apr 16. Epub 2021 Apr 16.

Department of Biliary-Pancreatic Surgery, Renji Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, 200127, China; Shanghai Key Laboratory of Biliary Tract Disease Research, Shanghai, 200092, China; State Key Laboratory of Oncogenes and Related Genes, Shanghai, 200127, China; Shanghai Research Center of Biliary Tract Disease, Shanghai, 200092, China. Electronic address:

Dysfunction of Sirtuin 3 (SIRT3), an NAD-dependent histone deacetylase, impairs varied mitochondrial metabolic pathways in human cancer. Here, we explored suppressive activity of SIRT3 in the progression of gallbladder cancer (GBC). Expression levels of SIRT3 in patients with GBC were lower than those in the adjacent normal tissue. Read More

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Optimization of pre- treatments with Propidium Monoazide and PEMAX™ before real-time quantitative PCR for detection and quantification of viable Helicobacter pylori cells.

J Microbiol Methods 2021 Apr 16:106223. Epub 2021 Apr 16.

Biotechnology Department, Universitat Politècnica de València, 46022, Valencia, Spain. Electronic address:

Accurate detection of H. pylori in different environmental and clinical samples is essential for public health strtdudies. Now, a big effort is being made to design PCR methodologies that allow for the detection of viable and viable but non-culturable (VBNC) H. Read More

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Environmental estrogens inhibit the expression of insulin-like growth factor mRNAs in rainbow trout in vitro by altering activation of the JAK-STAT, AKT-PI3K, and ERK signaling pathways.

Gen Comp Endocrinol 2021 Apr 16:113792. Epub 2021 Apr 16.

Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA. Electronic address:

Environmental estrogens (EE) have been found to disrupt a host of developmental, reproductive, metabolic, and osmoregulatory process in a wide-range of animals, particularly those in aquatic ecosystems where such compounds concentrate. Previously, we showed that EE inhibited post-embryonic organismal growth of rainbow trout in vivo, but the precise mechanism(s) through which EE exert their growth inhibiting effects remain unknown. In this study, we used rainbow trout (Oncorhynchus mykiss) as a model to investigate the direct effects of 17β-estradiol (E2), β-sitosterol (βS), and 4-n-nonylphenol (NP) on the synthesis of insulin-like growth factors (IGFs) and to elucidate the mechanism(s) by which EEs exert such effects. Read More

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Synthesis and biological evaluation of new coumarin derivatives as cytotoxic agents.

Arch Pharm (Weinheim) 2021 Apr 19:e2100029. Epub 2021 Apr 19.

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

New coumarin derivatives 9a-f, 10a-e, and 11a-f were synthesized and evaluated for their cytotoxic activity against a human breast cancer cell line (MCF-7). All compounds exhibited good activity in the nanomolar range, using doxorubicin and erlotinib as positive controls. The most active compound 9d with IC of 21 nM was tested against the HCT-116, HepG-2, A549, and SGC-7901 cell lines, with IC values of 0. Read More

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The Prenatal Morphomechanic Impact of Agenesis of the Corpus Callosum on Human Brain Structure and Asymmetry.

Cereb Cortex 2021 Apr 19. Epub 2021 Apr 19.

Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, 1090 Vienna, Austria.

Genetic, molecular, and physical forces together impact brain morphogenesis. The early impact of deficient midline crossing in agenesis of the Corpus Callosum (ACC) on prenatal human brain development and architecture is widely unknown. Here we analyze the changes of brain structure in 46 fetuses with ACC in vivo to identify their deviations from normal development. Read More

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SPRED proteins and their roles in signal transduction, development, and malignancy.

Genes Dev 2020 Nov;34(21-22):1410-1421

Helen Diller Family Comprehensive Cancer, University of California at San Francisco, San Francisco, California 94158, USA.

The roles of SPRED proteins in signaling, development, and cancer are becoming increasingly recognized. SPRED proteins comprise an N-terminal EVH-1 domain, a central c-Kit-binding domain, and C-terminal SROUTY domain. They negatively regulate signaling from tyrosine kinases to the Ras-MAPK pathway. Read More

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November 2020

Childhood Onset Strabismus: A Neurotrophic Factor Hypothesis.

J Binocul Vis Ocul Motil 2021 Apr 19:1-6. Epub 2021 Apr 19.

Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota.

Strabismus is a genetically heterogeneous disorder with complex molecular and neurophysiological causes. Evidence in the literature suggests a strong role for motor innervation in the etiology of strabismus, which connects central neural processes to the peripheral extraocular muscles. Current treatments of strabismus through surgery show that an inherent sensorimotor plasticity in the ocular motor system decreases the effectiveness of treatment, often driving eye alignment back toward its misaligned pre-surgical state by altering extraocular muscle tonus. Read More

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Carbon Arc Lamp Therapy Enhances the Repair Potential of Chronic Soft Tissue Injury in Rats Compared with the Ability of Photobiomodulation Therapy.

Photobiomodul Photomed Laser Surg 2021 Apr 19. Epub 2021 Apr 19.

Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Innovative Technology Research Institute of Tissue Repair and Regeneration, Key Laboratory of Regenerative Medicine, Ministry of Education, Guangzhou, P.R. China.

The effects of photobiomodulation therapy (PBMT) and carbon arc lamp therapy (CALT) on the repair of chronic soft tissue injury were compared. PBMT improves soft tissue repair of chronic injury. However, there has been no research on the effect of CALT. Read More

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The Bisindole Alkaloids Angustilongines M and A from Induce Mitochondrial Apoptosis and G0/G1 Cell Cycle Arrest in HT-29 Cells through Promotion of Tubulin Polymerization.

J Nat Prod 2021 Apr 19. Epub 2021 Apr 19.

Department of Chemistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.

A new linearly fused macroline-sarpagine bisindole, angustilongine M (), was isolated from the methanolic extract of . The structure of the alkaloid was elucidated based on analysis of the spectroscopic data, and its biological activity was evaluated together with another previously reported macroline-akuammiline bisindole from the same plant, angustilongine A (). Compounds and showed pronounced in vitro growth inhibitory activity against a wide panel of human cancer cell lines. Read More

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ALK Rearrangements Characterize 2 Distinct Types of Salivary Gland Carcinomas: Clinicopathologic and Molecular Analysis of 4 Cases and Literature Review.

Am J Surg Pathol 2021 Mar 22. Epub 2021 Mar 22.

*Institute of Pathology ¶Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Comprehensive Cancer Center (CCC) Erlangen-EMN, Erlangen §DERMPATH Muenchen ∥Institute of Pathology, Ludwig Maximilians University, Munich, Germany †Department of Pathology, Charles University, Faculty of Medicine in Plzen ‡Bioptic Laboratory Ltd, Plzen, Czech Republic #Department of Translational Research, University of Pisa, School of Medicine, Pisa, Italy.

The majority of salivary gland carcinomas are characterized by recurrent gene fusions that proved highly valuable diagnostically, but only rarely of therapeutic impact. Most of these fusion-positive carcinomas belong to the low-grade or intermediate-grade biological category. To date, only 5 cases of salivary gland carcinomas carrying an oncogenic ALK fusion have been reported in 4 recent studies, but their phenotypic spectrum and their nosological classification remain uncharacterized. Read More

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Growth hormone directly favors hepatic ketogenesis in persons with prediabetes or type 2 diabetes mellitus treated with empagliflozin.

Endocrine 2021 Apr 19. Epub 2021 Apr 19.

Post-graduation Program of Health Sciences, Londrina State University, Londrina, Brazil.

Purpose: Sodium-glucose cotransporter 2 inhibitors increase glucagon secretion by pancreatic alpha cells and the susceptibility to ketoacidosis. On the other hand, growth hormone (GH) stimulates peripheral lipolysis and provides free fatty acids (FFA) for ketogenesis; however, it remains unresolved whether GH directly impacts hepatic ketogenesis. We aimed to investigate the role of physiologic GH levels in promoting ketogenesis in prediabetic or type 2 diabetic patients under empagliflozin treatment. Read More

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Chemical composition of cell wall changes during developmental stages of galls on Matayba guianensis (Sapindaceae): perspectives obtained by immunocytochemistry analysis.

Naturwissenschaften 2021 Apr 19;108(3):16. Epub 2021 Apr 19.

Instituto de Biologia, Campus Umuarama, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, CEP 38402-020, Brazil.

The development of plant organs depends on cell division, elongation, structural and chemical changes, and reorganization of cell wall components. As phenotype manipulators, galling insects can manipulate the structure and metabolism of host tissues to build the gall. The gall formation depends on the rearrangement of cell wall components to allow cell growth and elongation, key step for the knowledge regarding gall development, and shape acquisition. Read More

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Transforming growth factor β as a possible independent factor in chronic hepatitis B.

Arch Virol 2021 Apr 19. Epub 2021 Apr 19.

Department of Hepatology Division 2, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.

To investigate the association between immune-cell-related cytokines and the development of chronic hepatitis B (CHB), patients with chronic hepatitis B virus (HBV) infection in the immunotolerant (IT) phase (n = 30) or hepatitis B envelope antigen (HBeAg)-positive CHB (n = 250) were enrolled in this study. Serological indicators and plasma cytokine levels were measured at the time of enrollment. The results showed that there were significant differences in the median age of the patients (27 vs. Read More

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Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes.

Arch Microbiol 2021 Apr 19. Epub 2021 Apr 19.

Institute of Nutritional and Food Science, Food Microbiology and Hygiene, University of Bonn, Friedrich-Hirzebruch-Allee 7, 53115, Bonn, Germany.

Listeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to - 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. Read More

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Streptomyces adelaidensis sp. nov., an actinobacterium isolated from the root of Callitris preissii with potential for plant growth-promoting properties.

Arch Microbiol 2021 Apr 19. Epub 2021 Apr 19.

Department of Medical Biotechnology, College of Medicine and Public Health, Flinders University, Adelaide, Australia.

An endophytic actinobacterium, strain CAP261 was isolated from the surface sterilized root of Callitris preissii (Australian native pine tree). As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Streptomyces. This strain was an aerobic actinobacterium with well-developed substrate mycelia with loop spore chains and the spore surfaces are verrucose. Read More

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Spatial and temporal localization of SPIRRIG and WAVE/SCAR reveal roles for these proteins in actin-mediated root hair development.

Plant Cell 2021 Apr 19. Epub 2021 Apr 19.

Noble Research Institute LLC, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73401.

Root hairs are single cell protrusions that enable roots to optimize nutrient and water acquisition. These structures attain their tubular shapes by confining growth to the cell apex, a process called tip growth. The actin cytoskeleton and endomembrane systems are essential for tip growth; however, little is known about how these cellular components coordinate their activities during this process. Read More

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Arabidopsis NF-YCs play dual roles in repressing brassinosteroid biosynthesis and signaling during light-regulated hypocotyl elongation.

Plant Cell 2021 Apr 19. Epub 2021 Apr 19.

Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Innovative Academy of Seed Design, Chinese Academy of Sciences, Guangzhou, China.

Light functions as the primary environmental stimulus and brassinosteroids (BRs) as important endogenous growth regulators throughout the plant lifecycle. Photomorphogenesis involves a series of vital developmental processes that require the suppression of BR-mediated seedling growth, but the mechanism underlying the light-controlled regulation of the BR pathway remain unclear. Here, we reveal that nuclear factor YC proteins (NF-YCs) function as essential repressors of the BR pathway during light-controlled hypocotyl growth in Arabidopsis thaliana. Read More

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Time of day prioritizes the pool of translating mRNAs in response to heat stress.

Plant Cell 2021 Apr 19. Epub 2021 Apr 19.

Department of Botany and Plant Sciences, University of California, Riverside, Riverside, California, USA.

The circadian clock helps organisms to anticipate and coordinate gene regulatory responses to changes in environmental stimuli. Under growth limiting temperatures, time of day modulates the accumulation of polyadenylated mRNAs. In response to heat stress, plants will conserve energy and selectively translate mRNAs. Read More

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Reverse plasticity underlies rapid evolution by clonal selection within populations of fibroblasts propagated on a novel soft substrate.

Mol Biol Evol 2021 Apr 19. Epub 2021 Apr 19.

Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station TX 77843, USA.

Mechanical properties such as substrate stiffness are a ubiquitous feature of a cell's environment. Many types of animal cells exhibit canonical phenotypic plasticity when grown on substrates of differing stiffness, in vitro and in vivo. Whether such plasticity is a multivariate optimum due to hundreds of millions of years of animal evolution, or instead is a compromise between conflicting selective demands, is unknown. Read More

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Improved diet quality is associated with decreased concentrations of inflammatory markers in adults with uncontrolled asthma.

Am J Clin Nutr 2021 Apr 19. Epub 2021 Apr 19.

Department of Medicine, University of Illinois at Chicago, Chicago, IL, USA.

Background: Asthma has become one of the major public health challenges, and recent studies show promising clinical benefits of dietary interventions, such as the Dietary Approaches to Stop Hypertension (DASH) diet.

Objective: The objective of this study was to examine whether changes in diet quality are associated with changes in inflammatory markers important in asthma pathophysiology.

Methods: In this exploratory study in patients with poorly controlled asthma participating in a randomized controlled trial of a DASH intervention study, changes in concentrations of a broad panel of serum proteins (51-plex Luminex assay, Affymetrix) were determined, and their relation to diet quality (DASH score) assessed by combining data of both intervention and usual-care control groups. Read More

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Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells.

J Vis Exp 2021 Apr 5(170). Epub 2021 Apr 5.

Department of Cell and Developmental Biology and the Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus;

The sense of taste is mediated by taste buds on the tongue, which are composed of rapidly renewing taste receptor cells (TRCs). This continual turnover is powered by local progenitor cells and renders taste function prone to disruption by a multitude of medical treatments, which in turn severely impacts the quality of life. Thus, studying this process in the context of drug treatment is vital to understanding if and how taste progenitor function and TRC production are affected. Read More

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Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity.

J Vis Exp 2021 Mar 31(169). Epub 2021 Mar 31.

Department of Chemical and Biomolecular Engineering, Lehigh University;

The cell wall of Gram-negative bacteria consists of an inner (cytoplasmic) and outer membrane (OM), separated by a thin peptidoglycan layer. Throughout growth, the outer membrane can bleb to form spherical outer membrane vesicles (OMVs). These OMVs are involved in numerous cellular functions including cargo delivery to host cells and communication with bacterial cells. Read More

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A novel -pimarane-type diterpenoid from with anti-inflammatory activity.

J Asian Nat Prod Res 2021 Apr 19:1-11. Epub 2021 Apr 19.

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.

A novel -pimarane-type diterpenoid, sigesbeckia J (), along with two known diterpenoids, siegesbeckia acid () and -18-acetoxy-16,17-dihydroxykauran-19-oic acid (), were isolated from the aerial parts of Makino. Their chemical structures were elucidated based on extensive spectroscopic interpretation. The absolute configuration of -pimarane-type diterpenoid () was determined by comparing experimental and calculated ECD spectra. Read More

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Adipose-derived stem cell exosomes facilitate rotator cuff repair by mediating tendon-derived stem cells.

Regen Med 2021 Apr 19. Epub 2021 Apr 19.

Department of Joint Surgery, Shanghai East Hospital, Tongji University, School of Medicine, Shanghai, 200120, PR China.

To evaluate the potential capability of adipose-derived stem cell exosomes (ADSC-exos) on rotator cuff repair by mediating the tendon-derived stem cells (TDSCs) and explored the mechanism. First, we investigated the growth, survival and migration of TDSCs in the presence of ADSC-exos . Using a rat rotator cuff injury model to analyze the ability of the ADSC-exos to promote rotator cuff healing . Read More

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