114,590 results match your criteria cd8 cells


Gene expression profiles for an immunoscore model in bone and soft tissue sarcoma.

Aging (Albany NY) 2021 May 4;13. Epub 2021 May 4.

Department of Orthopedics, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Background: Immune infiltration is a prognostic marker to clinical outcomes in various solid tumors. However, reports that focus on bone and soft tissue sarcoma are rare. The study aimed to analyze and identify how immune components influence prognosis and develop a novel prognostic system for sarcomas. Read More

View Article and Full-Text PDF

Rutaecarpine administration inhibits cancer cell growth in allogenic TRAMP-C1 prostate cancer mice correlating with immune balance in vivo.

Biomed Pharmacother 2021 May 1;139:111648. Epub 2021 May 1.

Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan.

Background: Rutaecarpine (Rut) is a plant alkaloid abundant in Euodia ruticarpa which is a Chinese herbal medicine used for treating various cancers. However, the Rut administration effect on prostate cancer in vivo remains unclear.

Aim: In the present study we established an allogenic TRAMP-C1 prostate cancer mouse model to evaluate the Rut administration effect and mechanism in vivo. Read More

View Article and Full-Text PDF

IL-2Rβγ signalling in lymphocytes promotes systemic inflammation and reduces plasma cholesterol in atherosclerotic mice.

Atherosclerosis 2021 Apr 24;326:1-10. Epub 2021 Apr 24.

Department of Clinical Sciences, Lund University, Skåne University Hospital, 20502, Malmö, Sweden. Electronic address:

Background And Aims: The relationship between inflammation and lipid metabolism is complex and bidirectional. Lymphocyte-driven inflammation has been shown to modulate both atherosclerotic plaque development and cholesterol levels, but the mechanisms are incompletely understood.

Methods: The cardiometabolic effects of IL-2Rβγ signalling in atherosclerotic Apoe mice were investigated by treatment with an agonistic IL-2Rβγ-targeting IL-2/anti-IL-2 complex or a monoclonal anti-CD122 (IL-2Rβ) blocking antibody. Read More

View Article and Full-Text PDF

CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance.

Cell Stem Cell 2021 Apr 28. Epub 2021 Apr 28.

Laboratory of Molecular Signaling, Division of Oral Biology and Medicine, School of Dentistry, Jonsson Comprehensive Cancer Center and Broad Stem Cell Research Center, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address:

Immunosurveillance is a critical mechanism guarding against tumor development and progression. Checkpoint inhibitors have shown significant success in cancer treatment, but expression of key factors such as PD-L1 in putative cancer stem cell (CSC) populations in squamous cell carcinoma has been inconclusive, suggesting that CSCs may have developed other mechanisms to escape immune surveillance. Here we show that CSCs upregulate the immune checkpoint molecule CD276 (B7-H3) to evade host immune responses. Read More

View Article and Full-Text PDF

CD8 T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope cross-react with selective seasonal coronaviruses.

Immunity 2021 Apr 13. Epub 2021 Apr 13.

Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia. Electronic address:

Efforts are being made worldwide to understand the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for the coronavirus disease 2019 (COVID-19) pandemic, including the impact of T cell immunity and cross-recognition with seasonal coronaviruses. Screening of SARS-CoV-2 peptide pools revealed that the nucleocapsid (N) protein induced an immunodominant response in HLA-B7 COVID-19-recovered individuals that was also detectable in unexposed donors. A single N-encoded epitope that was highly conserved across circulating coronaviruses drove this immunodominant response. Read More

View Article and Full-Text PDF

Identification of CDC20 as an immune infiltration-correlated prognostic biomarker in hepatocellular carcinoma.

Invest New Drugs 2021 May 3. Epub 2021 May 3.

Department of Hepatobiliary Surgery, Clinical Medical College, Yangzhou University, 98 West Nantong Rd, 225009, Yangzhou, Jiangsu, China.

Hepatocellular carcinoma (HCC) is a malignancy with a poor prognosis. E3 ubiquitin-protein ligases play essential roles in HCC, such as regulating progression, migration, and metastasis. We aimed to explore a hub E3 ubiquitin-protein ligase gene and verify its association with prognosis and immune cell infiltration in HCC. Read More

View Article and Full-Text PDF

MHC class II tetramers engineered for enhanced binding to CD4 improve detection of antigen-specific T cells.

Nat Biotechnol 2021 May 3. Epub 2021 May 3.

Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN, USA.

The ability to identify T cells that recognize specific peptide antigens bound to major histocompatibility complex (MHC) molecules has enabled enumeration and molecular characterization of the lymphocytes responsible for cell-mediated immunity. Fluorophore-labeled peptide:MHC class I (p:MHCI) tetramers are well-established reagents for identifying antigen-specific CD8 T cells by flow cytometry, but efforts to extend the approach to CD4 T cells have been less successful, perhaps owing to lower binding strength between CD4 and MHC class II (MHCII) molecules. Here we show that p:MHCII tetramers engineered by directed evolution for enhanced CD4 binding outperform conventional tetramers for the detection of cognate T cells. Read More

View Article and Full-Text PDF

USP12 promotes CD4 T cell responses through deubiquitinating and stabilizing BCL10.

Cell Death Differ 2021 May 3. Epub 2021 May 3.

Institute of Molecular Immunology, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.

Deubiquitinases (DUBs) regulate diverse biological processes and represent a novel class of drug targets. However, the biological function of only a small fraction of DUBs, especially in adaptive immune response regulation, is well-defined. In this study, we identified DUB ubiquitin-specific peptidase 12 (USP12) as a critical regulator of CD4 T cell activation. Read More

View Article and Full-Text PDF

Immune cell profiling of COVID-19 patients in the recovery stage by single-cell sequencing.

Cell Discov 2020 May 4;6(1):31. Epub 2020 May 4.

National Center for Liver Cancer, Second Military Medical University, 200438, Shanghai, China.

COVID-19, caused by SARS-CoV-2, has recently affected over 1,200,000 people and killed more than 60,000. The key immune cell subsets change and their states during the course of COVID-19 remain unclear. We sought to comprehensively characterize the transcriptional changes in peripheral blood mononuclear cells during the recovery stage of COVID-19 by single-cell RNA sequencing technique. Read More

View Article and Full-Text PDF

Cross-Sectional Study of the Effects of Job Burnout on Immune Function in 105 Female Oncology Nurses at a Tertiary Oncology Hospital.

Med Sci Monit 2021 May 4;27:e929711. Epub 2021 May 4.

Nursing Department, Guangxi Medical University Affiliated Cancer Hospital, Nanning, Guangxi, China (mainland).

BACKGROUND Nurses who work in hospitals experience a high level of burnout and the relationship between immune variables and burnout syndrome has yet to be elucidated. The aim of the present study was to investigate the effects of job burnout on immune function in female oncology nurses in a tertiary oncology hospital in Guangxi, China. The aspects of the human immune system evaluated were humoral and cellular immunity and complement components 3 (C3) and 4 (C4). Read More

View Article and Full-Text PDF

Cutting Edge: TCR-β Selection Is Required at the CD4CD8 Stage of Human T Cell Development.

J Immunol 2021 May 3. Epub 2021 May 3.

Department of Immunology, University of Toronto, Toronto, Ontario, Canada

T cell development is predicated on the successful rearrangement of the TCR gene loci, which encode for Ag-specific receptors. Recombination-activating gene (RAG) 2 is required for TCR gene rearrangements, which occur during specific stages of T cell development. In this study, we differentiated human pluripotent stem cells with a CRISPR/Cas9-directed deletion of the gene (RAG2-KO) to elucidate the requirement for the TCR β-chain in mediating β-selection during human T cell development. Read More

View Article and Full-Text PDF

Inhibition of CDK4/6 promotes CD8 T-cell memory formation.

Cancer Discov 2021 May 3. Epub 2021 May 3.

Dana-Farber Cancer Institute

CDK4/6 inhibitors are approved to treat breast cancer and are in trials for other malignancies. We examined CDK4/6 inhibition in mouse and human CD8 T cells during early stages of activation. Mice receiving tumor-specific CD8 T cells treated with CDK4/6 inhibitors displayed increased T cell persistence and immunologic memory. Read More

View Article and Full-Text PDF

The genetic variability, phylogeny and functional significance of E6, E7 and LCR in human papillomavirus type 52 isolates in Sichuan, China.

Virol J 2021 May 3;18(1):94. Epub 2021 May 3.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, People's Republic of China.

Background: Variations in human papillomavirus (HPV) E6 and E7 have been shown to be closely related to the persistence of the virus and the occurrence and development of cervical cancer. Long control region (LCR) of HPV has been shown multiple functions on regulating viral transcription. In recent years, there have been reports on E6/E7/LCR of HPV-16 and HPV-58, but there are few studies on HPV-52, especially for LCR. Read More

View Article and Full-Text PDF

An orally available PD-1/PD-L1 blocking peptide OPBP-1-loaded trimethyl chitosan hydrogel for cancer immunotherapy.

J Control Release 2021 Apr 30. Epub 2021 Apr 30.

School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, China; School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

Blockade of the immune checkpoint PD-1/PD-L1 with monoclonal antibodies demonstrated unprecedented clinical efficacies in many cancers. But the orally available low molecular weight inhibitors remain infancy. Compared to small molecules, peptide exhibits better selectivity and fewer side effects, but poor half-life and a big challenge to be orally administrated. Read More

View Article and Full-Text PDF

Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.

PLoS One 2021 3;16(5):e0249366. Epub 2021 May 3.

Embrapa Suínos e Aves, Concórdia, SC, Brazil.

Piglets are highly vulnerable to infections, but colostrum provides them with some protection. The function of colostrum components is unknown, as is if the amount and subsets of leukocytes in colostrum differ between gilts and sows. This study serially characterized leukocyte populations in colostrum for differential leukocyte counts. Read More

View Article and Full-Text PDF

CD8-positive peripheral T cell lymphoma in a patient following long-term nivolumab for advanced lung adenocarcinoma: A case report.

Thorac Cancer 2021 May 3. Epub 2021 May 3.

Second Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan.

A 54-year-old male smoker presented with hemoptysis. Advanced lung adenocarcinoma, cT1cN3M1b, stage IVB, was diagnosed. Enlargement of multiple intraperitoneal and inguinal lymph nodes and peripheral atypical lymphocytosis appeared after 33 cycles of second-line treatment with nivolumab, and a specimen obtained by left inguinal lymph node biopsy showed peripheral T cell lymphoma (PTCL), not otherwise specified. Read More

View Article and Full-Text PDF

Senescence-associated β-galactosidase reveals the abundance of senescent CD8+ T cells in aging humans.

Aging Cell 2021 May 3:e13344. Epub 2021 May 3.

Center for Cell Signaling, Rutgers-New Jersey Medical School, Rutgers Biomedical and Health Sciences, Rutgers University, Newark, New Jersey, USA.

Aging leads to a progressive functional decline of the immune system, rendering the elderly increasingly susceptible to disease and infection. The degree to which immune cell senescence contributes to this decline remains unclear, however, since markers that label immune cells with classical features of cellular senescence accurately and comprehensively have not been identified. Using a second-generation fluorogenic substrate for β-galactosidase and multi-parameter flow cytometry, we demonstrate here that peripheral blood mononuclear cells (PBMCs) isolated from healthy humans increasingly display cells with high senescence-associated β-galactosidase (SA-βGal) activity with advancing donor age. Read More

View Article and Full-Text PDF

Surgical resection of extremely rare primary giant splenic angiomyolipoma: a case report.

Surg Case Rep 2021 May 3;7(1):110. Epub 2021 May 3.

Department of Surgery, Tohoku Rosai Hospital, 4-3-21, Dainohara, Aobaku, Sendai, Miyagi, 981-8563, Japan.

Background: Angiomyolipoma is a benign mesenchymal tumor that develops commonly in the kidney and rarely in other organs. The involvement of the spleen in angiomyolipoma is extremely rare, and only one such case has been reported in the English literature.

Case Presentation: A 27-year-old man presented with adenoid hyperplasia and bilateral palatal tonsillar hyperplasia. Read More

View Article and Full-Text PDF

Identification of TAPBPL as a novel negative regulator of T-cell function.

EMBO Mol Med 2021 May 3:e13404. Epub 2021 May 3.

Department of Allied Health Sciences, University of Connecticut, Storrs, CT, USA.

T cell stimulatory and inhibitory molecules are critical for the regulation of immune responses. In this study, we identify a novel T cell co-inhibitory molecule TAPBPL, whose amino acid sequence shares homology with known B7 family members. TAPBPL protein is expressed on resting and activated T cells, B cells, monocytes, and dendritic cells (DCs), as well as on some tumor tissues. Read More

View Article and Full-Text PDF

Helicobacter pylori induces gastric cancer via down-regulating miR-375 to inhibit dendritic cell maturation.

Helicobacter 2021 May 3:e12813. Epub 2021 May 3.

School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.

Background: Recent studies and clinical samples have demonstrated that Helicobacter pylori could induce the downregulation of miR-375 in the stomach and promote gastric carcinogenesis. However, whether the immune cells are affected by Helicobacter pylori due to the downregulation of miR-375 is unclear.

Materials And Methods: In this study, we constructed an overexpression and knockdown of miR-375 and Helicobacter pylori infection cell models in vitro. Read More

View Article and Full-Text PDF

Analysis of specific antibody and cellular immune response to first-dose measles vaccine Edmonston-Zagreb in 9-month-old infants.

Allergol Immunopathol (Madr) 2021 1;49(3):193-201. Epub 2021 May 1.

Bio Farma, Pasteur Street No. 28, Bandung 40161, West Java, Indonesia.

Background: Measles vaccinations have been suggested to provide immune protection and decreased measles incidence. However, there was a limited study evaluating how the measles vaccine elicits specific immune responses.

Objective: This study aimed to evaluate both humoral and cellular immunity to first-dose measles vaccine Edmonston-Zagreb (EZ) in 9-month-old Indonesian infants. Read More

View Article and Full-Text PDF

T cell plasticity in renal autoimmune disease.

Cell Tissue Res 2021 May 3. Epub 2021 May 3.

I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

The presence of immune cells is a morphological hallmark of rapidly progressive glomerulonephritis, a disease group that includes anti-glomerular basement membrane glomerulonephritis, lupus nephritis, and anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis. The cellular infiltrates include cells from both the innate and the adaptive immune responses. The latter includes CD4 and CD8 T cells. Read More

View Article and Full-Text PDF

Chronic Exposure to Continuous Brightness or Darkness Modulates Immune Responses and Ameliorates the Antioxidant Enzyme System in Male Rats.

Authors:
Amira Moustafa

Front Vet Sci 2021 15;8:621188. Epub 2021 Apr 15.

Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.

Circadian rhythms are considered vital regulators of immune functions. This study aims to elucidate the effects of chronic circadian disruption on immune functions, clock genes expression, and antioxidant enzymes levels in lymphoid tissues. Adult male Sprague-Dawley rats were subjected to a normal light/dark cycle or either continuous light (LL) or continuous dark (DD) for 8 weeks. Read More

View Article and Full-Text PDF

Application of Immune Infiltration Signature and Machine Learning Model in the Differential Diagnosis and Prognosis of Bone-Related Malignancies.

Front Cell Dev Biol 2021 15;9:630355. Epub 2021 Apr 15.

Department of Orthopedics, Musculoskeletal Tumor Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Bone-related malignancies, such as osteosarcoma, Ewing's sarcoma, multiple myeloma, and cancer bone metastases have similar histological context, but they are distinct in origin and biological behavior. We hypothesize that a distinct immune infiltrative microenvironment exists in these four most common malignant bone-associated tumors and can be used for tumor diagnosis and patient prognosis. After sample cleaning, data integration, and batch effect removal, we used 22 publicly available datasets to draw out the tumor immune microenvironment using the ssGSEA algorithm. Read More

View Article and Full-Text PDF

Immune Profile in Patients With COVID-19: Lymphocytes Exhaustion Markers in Relationship to Clinical Outcome.

Front Cell Infect Microbiol 2021 15;11:646688. Epub 2021 Apr 15.

Centre for Primary Immunodeficiencies, Clinic of Pneumology and Phthisiology, Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin University Hospital, Martin, Slovakia.

The velocity of the COVID-19 pandemic spread and the variable severity of the disease course has forced scientists to search for potential predictors of the disease outcome. We examined various immune parameters including the markers of immune cells exhaustion and activation in 21 patients with COVID-19 disease hospitalised in our hospital during the first wave of the COVID-19 pandemic in Slovakia. The results showed significant progressive lymphopenia and depletion of lymphocyte subsets (CD3, CD4, CD8 and CD19) in correlation to the disease severity. Read More

View Article and Full-Text PDF

Combination of Bempegaldesleukin and Anti-CTLA-4 Prevents Metastatic Dissemination After Primary Resection or Radiotherapy in a Preclinical Model of Non-Small Cell Lung Cancer.

Front Oncol 2021 15;11:645352. Epub 2021 Apr 15.

Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, United States.

Surgical resection or hypo-fractionated radiation therapy (RT) in early-stage non-small cell lung cancer (NSCLC) achieves local tumor control, but metastatic relapse remains a challenge. We hypothesized that immunotherapy with anti-CTLA-4 and bempegaldesleukin (BEMPEG; NKTR-214), a CD122-preferential IL2 pathway agonist, after primary tumor RT or resection would reduce metastases in a syngeneic murine NSCLC model. Mice bearing Lewis Lung Carcinoma (LLC) tumors were treated with combinations of BEMPEG, anti-CTLA-4, and primary tumor treatment (surgical resection or RT). Read More

View Article and Full-Text PDF

CHI3L2 Is a Novel Prognostic Biomarker and Correlated With Immune Infiltrates in Gliomas.

Front Oncol 2021 15;11:611038. Epub 2021 Apr 15.

Department of Pathology, Sun Yat-Sen University Cancer Center, Guangzhou, China.

CHI3L2 (Chitinase-3-Like Protein 2) is a member of chitinase-like proteins (CLPs), which belong to the glycoside hydrolase 18 family. Its homologous gene, CHI3L1, has been extensively studied in various tumors and has been shown to be related to immune infiltration in breast cancer and glioblastoma. High CHI3L2 expression was reported to be associated with poor prognosis in breast cancer and renal cell carcinoma. Read More

View Article and Full-Text PDF

CD137: A Member of the TNFR Family - in Psoriasis Skin Lesions in Comparison with Normal Skin Specimens.

Iran J Pathol 2021 20;16(2):103-108. Epub 2020 Dec 20.

Department of Pathology, Faculty of Medical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Background & Objective: CD137 is a member of the TNF-Receptor family. TNF-alpha antagonists have therapeutic effect in active psoriasis. In this study, the relative frequency of CD137 expression was investigated in the inflammatory cells of psoriasis lesions for the first time. Read More

View Article and Full-Text PDF
December 2020

Adapting T Cell Receptor Ligand Discrimination Capability LAT.

Front Immunol 2021 16;12:673196. Epub 2021 Apr 16.

Division of Rheumatology, Rosalind Russell and Ephraim P. Engleman Arthritis Research Center, Department of Medicine, University of California, San Francisco, San Francisco, CA, United States.

Self- and non-self ligand discrimination is a core principle underlying T cell-mediated immunity. Mature αβ T cells can respond to a foreign peptide ligand presented by major histocompatibility complex molecules (pMHCs) on antigen presenting cells, on a background of continuously sensed self-pMHCs. How αβ T cells can properly balance high sensitivity and high specificity to foreign pMHCs, while surrounded by a sea of self-peptide ligands is not well understood. Read More

View Article and Full-Text PDF

Endoplasmic Reticulum-Mitochondria Crosstalk and Beta-Cell Destruction in Type 1 Diabetes.

Front Immunol 2021 16;12:669492. Epub 2021 Apr 16.

Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands.

Beta-cell destruction in type 1 diabetes (T1D) results from the combined effect of inflammation and recurrent autoimmunity. In response to inflammatory signals, beta-cells engage adaptive mechanisms where the endoplasmic reticulum (ER) and mitochondria act in concert to restore cellular homeostasis. In the recent years it has become clear that this adaptive phase may trigger the development of autoimmunity by the generation of autoantigens recognized by autoreactive CD8 T cells. Read More

View Article and Full-Text PDF